Rw2G043090

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012328
Physical Location & Seq
Forward (+)
69447032 .. 69449369
2338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043090.1

Sequence Viewer

Length: 2058 bp
ATGGAAGTTGCAAGCCCACTAAAAGATAGTTCTTCTGTATCATCAAGCCCTTTCTCTTCTCCAAATGTTGGAGCCTTGCTCAAGATTAAGATCATTTCATGGACTCAAGAGACTGGTTTGCCTGTTTCTGTTAGTGTTCGAGTTGGCGGTAAGACCTTTAACCTTCACAAGTACCCAATATTTTCAAAAAGTGGGTATTTCAAGAAAAGGATGAATGAATCAGCTGAGGTTGAGCTACCACCAGACTTCCCTGGAGGGCCAGAGACCTTTGAGATGATTGCATTGTTTATCTATGGCTCCTCCACATTGATTGACCCTTTCAATGTGGTAGCCCTGAGATGTGCAGCTGAGTTTCTAGAAATGACAGAAGAATACTCTTCTGGCAACCTCTGCGAGCGCTTTGATCTCTACTTAAACCAAGTGGTCATGCAGAGTTGGAATGACACCTTAATAGTACTCCAAAAGTGCCAAACTTTGCTTCCATGGTCCGAAGACTTGTTAATCGTGAGCCGCTGCATTGAGTGCCTAGCATTCATGGCTTGTATGGAGATTCTTGATCCAGAGAGAAGGCGTGACACACCGGTACTCACACTGGACACATTGAGCACCCGAAATTGGAGCTGTGAAATGGGGAAGGAGATTATGAGTCAGGACCTCTGGATCAAGGATCTCATTGCTTTACCATTTGGATTCTTCAAGAGGATAATAGGGTCCTTGAGAAGACAAGGTATGAAAGAAAAGTATTTGAGCCCCATTATTGTTTTCTATACTAACAAATGGGTACTTTCAAAGAAGACAATCCAATTTTGGGAGAACTCTGGTCACAGAATTAGGGATGATCATAAAGTTTCAGTTATCCTCCAAGGTGTTCTTGATTTGCTTCCCAAGGGGGAGAAGGCTAGTCGAGCAATCCCCGTTGGGTTTTACTTTGCAATTCTCTCTACATCCCTTGAAGTAGGTTTGAAAGGTGATAGTAAGGCAAAGTTGGAAGAGCAGATTGTATCTCAACTGCACTTTGCCCAACTGGAAGACTTTATCTTTCCAAAAAGTGGGACTGAGTCAATATCTTCTAGCATGGAGCTGGCTACAATGGAGAGCATAATTTCAACATATGTATCAGCTAATTTGGACAGGGATCATAGCCTTTCTGCAGGCAACTCAATTGTTGCAGAACTGTGGGATGCATATCTTTCTCACATAGCTACTGATCCAAACATGCAGCCTAAAAGATTCATGGAACTCATCGAAAGAGTACCGATATCCTACAGACACAATCATGACAAACTCTACAGGGCAATGAACACCTTCTTGCAGGCACACGCAACTGTCTGCCAAGAAGAGAAGTGGGCAGTGTGCAAGTACCTCAACTGCCAAAAACTGTCACAAGAGGCATGCATTGAGGCTGTTCAAAATGAGTTGATGCCGTTGCGTTTGATTGTTCAAGCACTTTTCGTGCAGCAACTCAGCACCCACCAAGCTTTCAAAGAATGCTCAGATTCATTCCGATATGTGCACTGTGGAGAGTTTTCTGGGAATCTCTCAAGCACTAGATGCCCAAACTCCAAAAGCCAGAATCTAAGGGATAGTCCCAGTCCATACACAGATGGAGATGAGCCAGCTGGTAGCAGACCCTTGAGCTTTTTGCTACAGAAGGACCTGGTAAATCAGAGACCTGAGCTGTCAAGGAAAGAATATGAGTCAACAAGCTTCAGAATTCAGAATCTTGAACAAGAGCTCGTGTCCTTGAAGAGGAGTCTCCAATGGCAGAGCATTTCAAAGAAAAAAGAGCCAGTTTTAACTGCAGCACCGAGCACGAAATCATATGGTAAGGATAGTAGATCACTGAGCAAAAAGGGAAACCCACTTGGACAGACAACAGGTTGCATTGGCTCTGTGAATTTTGCTTCCCAAAGAAGGTATGCCAGTCGACTACTGAAGGTCCTTTGCCGAATTTCCTTGTTTGGAAGTAGAAAGCTAAAGAGAAAACCAGGTGCCCCCAGCCCATGGACTAAGCCACAGCAGCAGAATACTCATCAGAATAATATGTATGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

685

Amino Acids

77.57

Weight (kDa)

8.67

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 218 - 465 4.5e-65 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012328)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48130
fragaria_vesca FvH4_6g41250 FvH4_6g41250 FvH4_6g41250
malus_domestica MD09G1121300.v1.1 MD17G1112300.v1.1
prunus_persica Prupe.3G202400_v2.0.a1
pyrus_communis pycom09g04570 pycom17g10400
rosa_chinensis RchiOBHm_Chr2g0156801
rosa_laevigata RLG00000020943
rosa_multiflora Rmu_co8389443.1_g000001
rosa_roxburghii Rroxscaffold_2G00092710
rosa_rugosa Rorug02G0456300
rosa_samantha Rh2AG521900 Rh2BG535300 Rh2CG506800 Rh2DG544300
rosa_wichuraiana Rw2G043090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1991
AccB7I CCANNNNNTGG 3 cut(s) 68, 1049, 2004
AccI GTMKAC 1 cut(s) 1927
AciI CCGC 2 cut(s) 147, 511
AclWI GGATC 5 cut(s) 551, 668, 675, 1143, 1202
AcsI RAATTY 3 cut(s) 1713, 1897, 1950
AcuI CTGAAG 2 cut(s) 1693, 1955
AfaI GTAC 6 cut(s) 173, 456, 585, 783, 1254, 1361
AfeI AGCGCT 1 cut(s) 398
AfiI CCNNNNNNNGG 7 cut(s) 68, 615, 808, 1049, 1749, 1914, 2004
AgeI ACCGGT 1 cut(s) 580
AjnI CCWGG 3 cut(s) 250, 1656, 1987
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
Alw21I GWGCWC 4 cut(s) 608, 1515, 1737, 1814
Alw26I GTCTC 4 cut(s) 104, 257, 1663, 1760
Alw44I GTGCAC 1 cut(s) 1511
AlwI GGATC 5 cut(s) 551, 668, 675, 1143, 1202
Aor51HI AGCGCT 1 cut(s) 398
AoxI GGCC 1 cut(s) 257
ApaLI GTGCAC 1 cut(s) 1511
ApeKI GCWGC 6 cut(s) 344, 513, 1219, 1456, 1802, 2020
ApoI RAATTY 3 cut(s) 1713, 1897, 1950
AsiGI ACCGGT 1 cut(s) 580
Asp700I GAANNNNTTC 1 cut(s) 1304
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 6 cut(s) 257, 486, 652, 711, 1654, 1939
AsuHPI GGTGA 1 cut(s) 980
AvaII GGWCC 5 cut(s) 486, 652, 711, 1654, 1939
BaeGI GKGCMC 2 cut(s) 1515, 1996
BaeI ACNNNNGTAYC 2 cut(s) 1098, 1131
BanI GGYRCC 1 cut(s) 1991
BanII GRGCYC 2 cut(s) 752, 1737
BauI CACGAG 1 cut(s) 1736
BbsI GAAGAC 4 cut(s) 498, 727, 800, 1035
Bbv12I GWGCWC 4 cut(s) 608, 1515, 1737, 1814
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 6 cut(s) 356, 500, 1231, 1468, 1814, 2032
BccI CCATC 1 cut(s) 1598
BceAI ACGGC 1 cut(s) 1408
BciT130I CCWGG 3 cut(s) 252, 1658, 1989
BclI TGATCA 1 cut(s) 838
BcoDI GTCTC 4 cut(s) 104, 257, 1663, 1760
BfaI CTAG 5 cut(s) 356, 527, 900, 1071, 1548
BfmI CTRYAG 5 cut(s) 1149, 1264, 1288, 1646, 1800
BfoI RGCGCY 1 cut(s) 400
BisI GCNGC 7 cut(s) 345, 511, 514, 1220, 1457, 1803, 2021
BlsI GCNGC 7 cut(s) 346, 512, 515, 1221, 1458, 1804, 2022
BmcAI AGTACT 1 cut(s) 456
Bme1390I CCNGG 3 cut(s) 252, 1658, 1989
Bme18I GGWCC 5 cut(s) 486, 652, 711, 1654, 1939
BmgT120I GGNCC 6 cut(s) 257, 486, 652, 711, 1654, 1939
BmiI GGNNCC 4 cut(s) 73, 298, 712, 1993
BmrFI CCNGG 3 cut(s) 252, 1658, 1989
BmrI ACTGGG 1 cut(s) 1584
BmsI GCATC 3 cut(s) 1171, 1410, 1541
BmuI ACTGGG 1 cut(s) 1584
BpiI GAAGAC 4 cut(s) 498, 727, 800, 1035
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 2 cut(s) 225, 1674
BpuEI CTTGAG 5 cut(s) 65, 90, 736, 1525, 1654
BsaBI GATNNNNATC 2 cut(s) 89, 1185
BsaI GGTCTC 2 cut(s) 257, 1663
BsaJI CCNNGG 5 cut(s) 250, 482, 862, 885, 2003
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 615, 808, 1049, 1749, 1914, 2004
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 6 cut(s) 118, 597, 1029, 1590, 1790, 1923
Bse3DI GCAATG 2 cut(s) 672, 1302
Bse8I GATNNNNATC 2 cut(s) 89, 1185
BseBI CCWGG 3 cut(s) 252, 1658, 1989
BseDI CCNNGG 5 cut(s) 250, 482, 862, 885, 2003
BseGI GGATG 4 cut(s) 216, 841, 944, 1186
BseJI GATNNNNATC 2 cut(s) 89, 1185
BseLI CCNNNNNNNGG 7 cut(s) 68, 615, 808, 1049, 1749, 1914, 2004
BseMI GCAATG 2 cut(s) 672, 1302
BseMII CTCAG 8 cut(s) 216, 326, 339, 1047, 1477, 1506, 1665, 1835
BseNI ACTGG 6 cut(s) 118, 597, 1029, 1590, 1790, 1923
BseRI GAGGAG 2 cut(s) 289, 1765
BseSI GKGCMC 2 cut(s) 1515, 1996
BseXI GCAGC 6 cut(s) 356, 500, 1231, 1468, 1814, 2032
BseYI CCCAGC 1 cut(s) 1997
BsgI GTGCAG 3 cut(s) 363, 995, 1475
BshFI GGCC 1 cut(s) 259
BshNI GGYRCC 1 cut(s) 1991
BshTI ACCGGT 1 cut(s) 580
BsiHKAI GWGCWC 4 cut(s) 608, 1515, 1737, 1814
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 2 cut(s) 1066, 1572
BslI CCNNNNNNNGG 7 cut(s) 68, 615, 808, 1049, 1749, 1914, 2004
BsmAI GTCTC 4 cut(s) 104, 257, 1663, 1760
BsmFI GGGAC 2 cut(s) 1066, 1572
BsmI GAATGC 2 cut(s) 530, 1493
BsnI GGCC 1 cut(s) 259
Bso31I GGTCTC 2 cut(s) 257, 1663
Bsp1286I GDGCHC 6 cut(s) 608, 752, 1515, 1737, 1814, 1996
Bsp143I GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
Bsp19I CCATGG 2 cut(s) 482, 2003
BspACI CCGC 2 cut(s) 147, 511
BspANI GGCC 1 cut(s) 259
BspCNI CTCAG 8 cut(s) 217, 327, 340, 1048, 1476, 1505, 1666, 1836
BspHI TCATGA 1 cut(s) 1276
BspLI GGNNCC 4 cut(s) 73, 298, 712, 1993
BspMAI CTGCAG 2 cut(s) 1153, 1804
BspPI GGATC 5 cut(s) 551, 668, 675, 1143, 1202
BspQI GCTCTTC 1 cut(s) 984
BspT107I GGYRCC 1 cut(s) 1991
BspTNI GGTCTC 2 cut(s) 257, 1663
BsrDI GCAATG 2 cut(s) 672, 1302
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 6 cut(s) 118, 597, 1029, 1590, 1790, 1923
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 5 cut(s) 250, 482, 862, 885, 2003
BssMI GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
BssSI CACGAG 1 cut(s) 1736
BssT1I CCWWGG 4 cut(s) 482, 862, 885, 2003
Bst2BI CACGAG 1 cut(s) 1736
Bst2UI CCWGG 3 cut(s) 252, 1658, 1989
Bst4CI ACNGT 4 cut(s) 1176, 1327, 1380, 1517
Bst6I CTCTTC 5 cut(s) 61, 382, 984, 1332, 1742
BstAPI GCANNNNNTGC 3 cut(s) 390, 522, 1551
BstC8I GCNNGC 7 cut(s) 13, 395, 1083, 1153, 1314, 1393, 1617
BstDSI CCRYGG 2 cut(s) 482, 2003
BstENI CCTNNNNNAGG 1 cut(s) 1747
BstF5I GGATG 4 cut(s) 216, 841, 944, 1186
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 9 cut(s) 93, 406, 559, 663, 670, 841, 1138, 1210, 1841
BstMAI GTCTC 4 cut(s) 104, 257, 1663, 1760
BstMBI GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
BstMWI GCNNNNNNNGC 6 cut(s) 390, 522, 536, 905, 1551, 2020
BstNI CCWGG 3 cut(s) 252, 1658, 1989
BstNSI RCATGY 2 cut(s) 1219, 1395
BstSCI CCNGG 3 cut(s) 250, 1656, 1987
BstSFI CTRYAG 5 cut(s) 1149, 1264, 1288, 1646, 1800
BstSLI GKGCMC 2 cut(s) 1515, 1996
BstV1I GCAGC 6 cut(s) 356, 500, 1231, 1468, 1814, 2032
BstV2I GAAGAC 4 cut(s) 498, 727, 800, 1035
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 1 cut(s) 259
BtgI CCRYGG 2 cut(s) 482, 2003
BtsCI GGATG 4 cut(s) 216, 841, 944, 1186
BtsI GCAGTG 1 cut(s) 1356
BtsIMutI CAGTG 4 cut(s) 590, 1356, 1513, 1841
Cac8I GCNNGC 7 cut(s) 13, 395, 1083, 1153, 1314, 1393, 1617
CciI TCATGA 1 cut(s) 1276
CfoI GCGC 1 cut(s) 399
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 6 cut(s) 257, 486, 652, 711, 1654, 1939
CsiI ACCWGGT 2 cut(s) 1656, 1987
Csp6I GTAC 6 cut(s) 172, 455, 584, 782, 1253, 1360
CspAI ACCGGT 1 cut(s) 580
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviQI GTAC 6 cut(s) 172, 455, 584, 782, 1253, 1360
DpnI GATC 9 cut(s) 92, 405, 558, 662, 669, 840, 1137, 1209, 1840
DpnII GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
Eam1104I CTCTTC 5 cut(s) 61, 382, 984, 1332, 1742
EarI CTCTTC 5 cut(s) 61, 382, 984, 1332, 1742
Ecl136II GAGCTC 1 cut(s) 1735
Eco130I CCWWGG 4 cut(s) 482, 862, 885, 2003
Eco24I GRGCYC 2 cut(s) 752, 1737
Eco31I GGTCTC 2 cut(s) 257, 1663
Eco32I GATATC 1 cut(s) 1260
Eco47I GGWCC 5 cut(s) 486, 652, 711, 1654, 1939
Eco47III AGCGCT 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 1735
Eco57I CTGAAG 2 cut(s) 1693, 1955
EcoICRI GAGCTC 1 cut(s) 1735
EcoNI CCTNNNNNAGG 1 cut(s) 1747
EcoO109I RGGNCCY 4 cut(s) 652, 711, 1654, 1939
EcoRI GAATTC 1 cut(s) 1713
EcoRII CCWGG 3 cut(s) 250, 1656, 1987
EcoRV GATATC 1 cut(s) 1260
EcoT14I CCWWGG 4 cut(s) 482, 862, 885, 2003
EcoT22I ATGCAT 2 cut(s) 1186, 1397
EcoT38I GRGCYC 2 cut(s) 752, 1737
ErhI CCWWGG 4 cut(s) 482, 862, 885, 2003
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FaqI GGGAC 2 cut(s) 1066, 1572
FauNDI CATATG 2 cut(s) 1111, 1822
FbaI TGATCA 1 cut(s) 838
FblI GTMKAC 1 cut(s) 1927
Fnu4HI GCNGC 7 cut(s) 345, 511, 514, 1220, 1457, 1803, 2021
FokI GGATG 4 cut(s) 223, 848, 931, 1193
FriOI GRGCYC 2 cut(s) 752, 1737
Fsp4HI GCNGC 7 cut(s) 345, 511, 514, 1220, 1457, 1803, 2021
FspBI CTAG 5 cut(s) 356, 527, 900, 1071, 1548
GlaI GCGC 1 cut(s) 398
GluI GCNGC 7 cut(s) 345, 511, 514, 1220, 1457, 1803, 2021
GsaI CCCAGC 1 cut(s) 2001
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 1 cut(s) 259
HapII CCGG 1 cut(s) 581
HhaI GCGC 1 cut(s) 399
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 2 cut(s) 1701, 1928
HindII GTYRAC 2 cut(s) 1701, 1928
HindIII AAGCTT 2 cut(s) 1476, 1705
HpaII CCGG 1 cut(s) 581
HphI GGTGA 1 cut(s) 980
Hpy166II GTNNAC 3 cut(s) 1513, 1701, 1928
Hpy188I TCNGA 7 cut(s) 490, 1495, 1505, 1668, 1712, 1719, 2037
Hpy8I GTNNAC 3 cut(s) 1513, 1701, 1928
HpyAV CCTTC 8 cut(s) 173, 561, 628, 889, 1315, 1645, 1908, 1930
HpyCH4III ACNGT 4 cut(s) 1176, 1327, 1380, 1517
HpyF10VI GCNNNNNNNGC 6 cut(s) 390, 522, 536, 905, 1551, 2020
HspAI GCGC 1 cut(s) 397
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 4 cut(s) 71, 302, 618, 1078
Lsp1109I GCAGC 6 cut(s) 356, 500, 1231, 1468, 1814, 2032
LweI GCATC 3 cut(s) 1171, 1410, 1541
MabI ACCWGGT 2 cut(s) 1656, 1987
MaeI CTAG 5 cut(s) 356, 527, 900, 1071, 1548
MaeIII GTNAC 3 cut(s) 572, 821, 1380
MalI GATC 9 cut(s) 92, 405, 558, 662, 669, 840, 1137, 1209, 1840
MboI GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 6 cut(s) 608, 752, 1515, 1737, 1814, 1996
MlyI GAGTC 5 cut(s) 97, 655, 1067, 1706, 1762
MmeI TCCRAC 3 cut(s) 49, 416, 966
Mph1103I ATGCAT 2 cut(s) 1186, 1397
MroXI GAANNNNTTC 1 cut(s) 1304
MseI TTAA 6 cut(s) 87, 159, 413, 449, 500, 1796
MslI CAYNNNNRTG 1 cut(s) 1275
MspA1I CMGCKG 4 cut(s) 224, 347, 513, 1619
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 3 cut(s) 252, 1658, 1989
MunI CAATTG 1 cut(s) 1161
Mva1269I GAATGC 2 cut(s) 530, 1493
MvaI CCWGG 3 cut(s) 252, 1658, 1989
MwoI GCNNNNNNNGC 6 cut(s) 390, 522, 536, 905, 1551, 2020
NcoI CCATGG 2 cut(s) 482, 2003
NdeI CATATG 2 cut(s) 1111, 1822
NdeII GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
NlaIV GGNNCC 4 cut(s) 73, 298, 712, 1993
NmuCI GTSAC 3 cut(s) 572, 821, 1380
NsiI ATGCAT 2 cut(s) 1186, 1397
NspI RCATGY 2 cut(s) 1219, 1395
PaeI GCATGC 1 cut(s) 1395
PagI TCATGA 1 cut(s) 1276
PciSI GCTCTTC 1 cut(s) 984
PctI GAATGC 2 cut(s) 530, 1493
PdmI GAANNNNTTC 1 cut(s) 1304
PfeI GAWTC 8 cut(s) 218, 550, 690, 1230, 1496, 1534, 1573, 1720
PflFI GACNNNGTC 1 cut(s) 1057
PflMI CCANNNNNTGG 3 cut(s) 68, 1049, 2004
PinAI ACCGGT 1 cut(s) 580
PkrI GCNGC 7 cut(s) 346, 512, 515, 1221, 1458, 1804, 2022
PleI GAGTC 5 cut(s) 97, 654, 1066, 1705, 1761
PpsI GAGTC 5 cut(s) 97, 654, 1066, 1705, 1761
PpuMI RGGWCCY 4 cut(s) 652, 711, 1654, 1939
Psp124BI GAGCTC 1 cut(s) 1737
Psp5II RGGWCCY 4 cut(s) 652, 711, 1654, 1939
Psp6I CCWGG 3 cut(s) 250, 1656, 1987
PspFI CCCAGC 1 cut(s) 1997
PspGI CCWGG 3 cut(s) 250, 1656, 1987
PspN4I GGNNCC 4 cut(s) 73, 298, 712, 1993
PspPI GGNCC 6 cut(s) 257, 486, 652, 711, 1654, 1939
PspPPI RGGWCCY 4 cut(s) 652, 711, 1654, 1939
PstI CTGCAG 2 cut(s) 1153, 1804
PsuI RGATCY 1 cut(s) 667
PsyI GACNNNGTC 1 cut(s) 1057
PvuII CAGCTG 3 cut(s) 224, 347, 1619
RsaI GTAC 6 cut(s) 173, 456, 585, 783, 1254, 1361
RsaNI GTAC 6 cut(s) 172, 455, 584, 782, 1253, 1360
RseI CAYNNNNRTG 1 cut(s) 1275
SacI GAGCTC 1 cut(s) 1737
SalI GTCGAC 1 cut(s) 1926
SapI GCTCTTC 1 cut(s) 984
SaqAI TTAA 6 cut(s) 87, 159, 413, 449, 500, 1796
SatI GCNGC 7 cut(s) 345, 511, 514, 1220, 1457, 1803, 2021
Sau3AI GATC 9 cut(s) 90, 403, 556, 660, 667, 838, 1135, 1207, 1838
Sau96I GGNCC 6 cut(s) 257, 486, 652, 711, 1654, 1939
ScaI AGTACT 1 cut(s) 456
SchI GAGTC 5 cut(s) 97, 655, 1067, 1706, 1762
ScrFI CCNGG 3 cut(s) 252, 1658, 1989
SduI GDGCHC 6 cut(s) 608, 752, 1515, 1737, 1814, 1996
SexAI ACCWGGT 2 cut(s) 1656, 1987
SfaNI GCATC 3 cut(s) 1171, 1410, 1541
SfcI CTRYAG 5 cut(s) 1149, 1264, 1288, 1646, 1800
SinI GGWCC 5 cut(s) 486, 652, 711, 1654, 1939
SmiMI CAYNNNNRTG 1 cut(s) 1275
SmlI CTYRAG 5 cut(s) 80, 105, 715, 1540, 1633
SmoI CTYRAG 5 cut(s) 80, 105, 715, 1540, 1633
SphI GCATGC 1 cut(s) 1395
SsiI CCGC 2 cut(s) 147, 511
SspI AATATT 1 cut(s) 180
SspMI CTAG 5 cut(s) 356, 527, 900, 1071, 1548
SstI GAGCTC 1 cut(s) 1737
StyD4I CCNGG 3 cut(s) 250, 1656, 1987
StyI CCWWGG 4 cut(s) 482, 862, 885, 2003
TaaI ACNGT 4 cut(s) 1176, 1327, 1380, 1517
TaqI TCGA 4 cut(s) 139, 904, 1245, 1927
TatI WGTACW 1 cut(s) 454
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 8 cut(s) 218, 550, 690, 1230, 1496, 1534, 1573, 1720
Tru1I TTAA 6 cut(s) 87, 159, 413, 449, 500, 1796
Tru9I TTAA 6 cut(s) 87, 159, 413, 449, 500, 1796
TscAI CASTG 4 cut(s) 597, 1356, 1520, 1848
TseFI GTSAC 3 cut(s) 572, 821, 1380
TseI GCWGC 6 cut(s) 344, 513, 1219, 1456, 1802, 2020
Tsp45I GTSAC 3 cut(s) 572, 821, 1380
TspDTI ATGAA 8 cut(s) 87, 227, 231, 523, 746, 1222, 1313, 1488
TspRI CASTG 4 cut(s) 597, 1356, 1520, 1848
Tth111I GACNNNGTC 1 cut(s) 1057
Van91I CCANNNNNTGG 3 cut(s) 68, 1049, 2004
VneI GTGCAC 1 cut(s) 1511
VpaK11BI GGWCC 5 cut(s) 486, 652, 711, 1654, 1939
XagI CCTNNNNNAGG 1 cut(s) 1747
XapI RAATTY 3 cut(s) 1713, 1897, 1950
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 2 cut(s) 1219, 1395
XmiI GTMKAC 1 cut(s) 1927
XmnI GAANNNNTTC 1 cut(s) 1304
XspI CTAG 5 cut(s) 356, 527, 900, 1071, 1548
ZrmI AGTACT 1 cut(s) 456
Zsp2I ATGCAT 2 cut(s) 1186, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.