MD09G1126400.v1.1

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013571
Physical Location & Seq
Reverse (-)
9761004 .. 9766933
5930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1126400.v1.1.491

Sequence Viewer

Length: 3387 bp
ATGGAGGGTAGAGAGATGTCGTCGGCGTCTTTGAACAAGATGGATAGTTCTAGAAGCAGCTCTAGTCAATCAAAACCTGGGGTTCATTTATTTTCGCAGACGCCCGTTGATGCGAAGCTTGACATAGACTTTCGAGAATCTCAACAGTTTTTTGATTACTCTGCTTCAGTTGACTGCAATATCTCATCTTCCACTAGCAAAATCCCCTCCAGCACTGTATCTGCTTATCTCCGAAGCATGCAGCGAGGAAGCCTTATTCAACCATTTGGTTGCTTGATTGCTGTTGATGAGAAAAACTTCACTGTTCTTGCCTATAGTGAAAATGCCCCAGAAATGTTGGACTTGGCCCCTCATGCTGTTCCAAATATCGAGCAGCAAGAAGCTTTGACTTTTGGAGCGGATGTGCAGACACTCTTTCAGTCCTCAGGTGCTGCTGCATTGCACAAAGCAGCCCAGTTTGGGGAAGTTAATCTTCTCAACCCCATATTGGTGCATTCTAAGACCTCCGGTAAGCCCTTTTATGCAATTCTACATCGAGTTGACGTTGGTTTAGTAATAGATTTGGAACCAGTAAGTCCAGCTGATGTTCCAGTGACTGCTGCGGGAGCATTGAAATCTTATAAGCTTGCAGCCAAAGCCATCTCAAAATTGCAGTCCTTGCCAAGTGGGGATATATCATTGTTATGTGATGTTATAGTTAAGGAAGTTAGTGATTTGACGGGTTATGATCGCATAATGGTGTATAAATTTCATGAAGATGAACACGGGGAAGTTATCGCTGAGTGCCATCAACCTGACCTGGAACCTTATCTGGGTTTTCATTTCCCAGCCACGGATATACCTCATGCTTCTAGATTCTTATTTATGAGGAACAAGGTTAGGATGATATGTGATTGTTTAGCCCCTCCAGTTAAAGTAATCCAGGACAAGAAATTAGCTCAACCATTGAGTCTTTGTGGATCAACATTGAGATCTCCTCATGGTTGCCATGCACTGTATATGAAAAGTATGTCTTCCATTGCATCTCTTGTGATGTCTGTTACAATCAATGATGATGTTGATGAGATGGACAGTGATCAGCGGAAGGGAAGAAGAAAATTATGGGGCTTAGTGGTTTGCCATCACACAAGCCCTAGATTTGTTCCATTCCCTTTAAGATATGCCTGTGAGTTCTTGATACAAGTTTTTGGTGTACAGATCAGCAAAGAAGTGGAGCTGGCTGCTCAGTCGAGGGAGAAACATATATTAAAAATACAGACTCTGCTCTGTGACATGCTTATGAGGGATTCCCCTGTAGGGATTGTTACTCAATCGCCTAATGTGATGGACCTTGTTAAGTGTGATGGAGCTGCATTATATTACAGGAAGAAATTATGGTTGCTTGGCGTTACCCCGACAGAAGCACAAATTGAGGATATTGCTGAATGGCTTCTCAAGTACCATAGTGGGAGCACAGGCTTAACTACTGATAGCCTTATGGAGGCTGGTTATCTAGGTGCTTCAGCCCTTGGCGATGAAGTTTGCGGAATGGCTGCTATTAAGATTACCTCAAAGGATTTCCTTTTTTGGTTTCGGTCCCACACCGCAAAGGAAATCAAATGGGGTGGAGCAAAACATGATCCTGATGACAAGGATGATGGAAGAAAAATGCATCCAAGGTCATCATTCAAGGCTTTCCTGGAGGTGGTTAAGCGACGAAGCGTGCCTTGGGAAGATATTGAAATGGATGTCATCCATTCTTTGCAGCTAATACTGAGAGGATCATTACCTGACGAGCCTGTGGATGATTCCAAAGTGATTGTGAAGGCTCCATCAGTTGATGATAGGATACAGAGAGTGGATGAACTGCGCATTGTCACAAATGAAATGGTTCGACTTATTGAGACAGCTGTAGTTCCCATTTTTGCTGTTGATGCCTCCGGTACTATAAATGGGTGGAATACAAAAGCATCTGAGCTAACAGGGTTAGCTGTTGAGCAAGCTATTGGTATGCCGTTAGTTGATATTGTTGGAGATGATTCAATTGAAGTGGTGAAGGACATGCTCTCATTCGCATTGCAAGGTGTGGAGAAGAAAAATGTTGAAATCAAACTGAAAACATTTGGCCATCAAGAAAGTAACAGTTTTGTAATCTTGGTGGTCAATTCATGTTGTAGCCGAGATATAAAGGAAGATGTAGTTGGGGTTTGCTTTGTAAGCCAAGATCTTACCAAAGAAAAACTGGGTATGGACAAGTATACCCGTTTGCTAGGTGATTATGTTGGGATTGTTCGAAGCCCATCTGCACTGATTCCTCCTATATTTATGACTGATGAGAATTTTCGTTGCTTGGAGTGGAATGACGCTATGCAAAAATTGTCTGGCTTGAGAAGGGAAGAAGCAGTTGATCGGATGCTCATTGGGGAAGTTTTCACTGTGAAAAATTTTGGTTGTCGTGTTAAGGGTCATGACACTTTAACCAAGCTTAGCATTCTACTAAATGGGGTAATAACAGGCGAGAATGTTAGTAAATTGCATTTTGGGTTTTACGATCAGCAGGGTAATTATGTTGAAGCATTACTTTCTGCCAACAAACGAATTGATGCATTGGGAAAGATAACTGGGGTTTTGTTCTTTTTACATGTGGCTAGTCCAGAGCTTATGTATGCTATGCAGATGCAGAGAGCATCAGAGCAGGCTGCAGCTGATAGTATTAAGAAACTGGCATATGTTCGTCAAGAAATCAAAAAGCCTCTAAGTGGGATTATGCTTATGCATAATCTGATGGGGTCTTCTGATTTAAATGAAGAGCAGAAGCAGTTACTTAGGAAGAGAAGATTGTGTCAGGACCAATTGGCCAAGATTGTTGATGACACTGATCTCGAAAGTATCGAGGAATGCTACATGGAATTAAATTCTAGTGAATTTAACCTTGGGGAAACTGTGGAGGTTGTCATATATCAAGTTATGGTTTTGAGTCAGGAGCGGCAAGTGAAGGTCATCCATGACTCACCAGCCGAGGTGTCATCCATGCTCTTGTATGGAGACAATTTGAGGCTACAACAAATTCTGTCTGATTTCTTGACAAACACACTCCTTTTCACCCCTGCATCGGAAGGATTGTCCATTGGGCTCAGAGTAACCCCAAAGAAGGAACGTATAGGCATGAAAATGCACATTGTTCATCTTGAGTTCCGTATCACTCATCCAGCACCAGGAATTCCAGACGATCTAATTCAAGAGATGTTTCACAACAGCCATCGAGTCTCAAAGGAAGGGCTAGGCCTACAAATGAGCAAGAATCTGGTGAAGATCATGAACGGCACTGTATGTTATCAGAGAGAAGCAGAGAGAGCAGCCTTTATAATACTCATAGAATTTCCGCTGGTTCACCAGATTGGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1129

Amino Acids

125.64

Weight (kDa)

5.82

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 76 - 191 2.2e-36 PAS fold
GAF PF01590 225 - 403 3.4e-14 GAF domain
PHY PF00360 417 - 588 5.6e-55 Phytochrome region
PAS PF00989 622 - 735 1.1e-19 PAS fold
PAS PF00989 754 - 874 1.1e-13 PAS fold
HisKA PF00512 901 - 959 1.3e-08 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1008 - 1121 1e-11 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35840
fragaria_vesca FvH4_6g40650 FvH4_6g40650 FvH4_6g40650
malus_domestica MD09G1126400.v1.1
prunus_persica Prupe.3G198200_v2.0.a1 Prupe.3G198200_v2.0.a1
pyrus_communis pycom09g05070 pycom17g10740
rosa_chinensis RchiOBHm_Chr2g0156021
rosa_laevigata RLG00000020882
rosa_multiflora Rmu_sc0011922.1_g000002
rosa_rugosa Rorug02G0451300
rosa_samantha Rh2AG518100 Rh2BG529300 Rh2CG502800 Rh2DG538400
rosa_wichuraiana Rw2G042660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 621, 3344
Acc16I TGCGCA 1 cut(s) 1850
AccBSI CCGCTC 2 cut(s) 398, 2965
AccI GTMKAC 1 cut(s) 2237
AciI CCGC 7 cut(s) 398, 602, 1081, 1524, 1584, 2965, 3362
AclWI GGATC 3 cut(s) 967, 1613, 1768
AcoI YGGCCR 2 cut(s) 2104, 2835
AcsI RAATTY 8 cut(s) 746, 2317, 2422, 2893, 2903, 3045, 3198, 3356
AcuI CTGAAG 2 cut(s) 150, 1485
AcyI GRCGYC 2 cut(s) 26, 101
AfaI GTAC 3 cut(s) 1194, 1439, 1924
AflIII ACRYGT 1 cut(s) 2620
AjnI CCWGG 5 cut(s) 76, 798, 921, 1677, 3193
AjuI GAANNNNNNNTTGG 6 cut(s) 1690, 1722, 2163, 2195, 2895, 2927
Alw21I GWGCWC 1 cut(s) 1454
Alw26I GTCTC 3 cut(s) 1877, 3018, 3250
AlwI GGATC 3 cut(s) 967, 1613, 1768
AlwNI CAGNNNCTG 3 cut(s) 431, 596, 1261
AoxI GGCC 4 cut(s) 345, 2104, 2835, 3262
ApoI RAATTY 8 cut(s) 746, 2317, 2422, 2893, 2903, 3045, 3198, 3356
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp700I GAANNNNTTC 3 cut(s) 296, 1428, 1869
AspLEI GCGC 1 cut(s) 1851
AspS9I GGNCC 4 cut(s) 346, 1327, 1575, 2827
AsuHPI GGTGA 6 cut(s) 2044, 2264, 2982, 3073, 3298, 3362
AsuII TTCGAA 1 cut(s) 2272
AvaII GGWCC 3 cut(s) 1327, 1575, 2827
AxyI CCTNAGG 1 cut(s) 424
BalI TGGCCA 2 cut(s) 2106, 2837
BanII GRGCYC 1 cut(s) 3114
BbsI GAAGAC 2 cut(s) 1005, 2763
Bbv12I GWGCWC 1 cut(s) 1454
BceAI ACGGC 2 cut(s) 1978, 3316
BciT130I CCWGG 5 cut(s) 78, 800, 923, 1679, 3195
BciVI GTATCC 1 cut(s) 1821
BclI TGATCA 1 cut(s) 1075
BcoDI GTCTC 3 cut(s) 1877, 3018, 3250
BfmI CTRYAG 4 cut(s) 313, 1293, 1890, 2679
BfuI GTATCC 1 cut(s) 1821
BglII AGATCT 2 cut(s) 971, 2203
BlpI GCTNAGC 1 cut(s) 2465
Bme1390I CCNGG 5 cut(s) 78, 800, 923, 1679, 3195
Bme18I GGWCC 3 cut(s) 1327, 1575, 2827
BmgT120I GGNCC 4 cut(s) 346, 1327, 1575, 2827
BmiI GGNNCC 5 cut(s) 348, 567, 804, 1577, 1809
BmrFI CCNGG 5 cut(s) 78, 800, 923, 1679, 3195
BmrI ACTGGG 3 cut(s) 448, 2231, 2610
BmuI ACTGGG 3 cut(s) 448, 2231, 2610
BpiI GAAGAC 2 cut(s) 1005, 2763
BplI GAGNNNNNCTC 2 cut(s) 961, 993
BpmI CTGGAG 3 cut(s) 193, 891, 1700
Bpu1102I GCTNAGC 1 cut(s) 2465
Bpu14I TTCGAA 1 cut(s) 2272
BpuEI CTTGAG 3 cut(s) 1418, 2386, 3188
BsaHI GRCGYC 2 cut(s) 26, 101
BsaJI CCNNGG 7 cut(s) 77, 831, 1507, 1655, 1706, 2911, 2997
BsaWI WCCGGW 2 cut(s) 506, 1919
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bse1I ACTGG 7 cut(s) 454, 569, 590, 908, 2226, 2605, 2706
Bse21I CCTNAGG 1 cut(s) 424
Bse3DI GCAATG 3 cut(s) 437, 1017, 2054
BseBI CCWGG 5 cut(s) 78, 800, 923, 1679, 3195
BseDI CCNNGG 7 cut(s) 77, 831, 1507, 1655, 1706, 2911, 2997
BseMI GCAATG 3 cut(s) 437, 1017, 2054
BseMII CTCAG 6 cut(s) 438, 771, 1238, 1745, 1944, 3127
BseNI ACTGG 7 cut(s) 454, 569, 590, 908, 2226, 2605, 2706
BseRI GAGGAG 1 cut(s) 966
BseYI CCCAGC 1 cut(s) 826
BsgI GTGCAG 2 cut(s) 425, 2268
BshFI GGCC 4 cut(s) 347, 2106, 2837, 3264
BsiHKAI GWGCWC 1 cut(s) 1454
BsiSI CCGG 2 cut(s) 507, 1920
BslFI GGGAC 1 cut(s) 1561
BsmAI GTCTC 3 cut(s) 1877, 3018, 3250
BsmFI GGGAC 1 cut(s) 1561
BsmI GAATGC 3 cut(s) 493, 2469, 2882
BsnI GGCC 4 cut(s) 347, 2106, 2837, 3264
Bsp119I TTCGAA 1 cut(s) 2272
Bsp1286I GDGCHC 2 cut(s) 1454, 3114
Bsp1407I TGTACA 1 cut(s) 1192
Bsp1720I GCTNAGC 1 cut(s) 2465
BspACI CCGC 7 cut(s) 398, 602, 1081, 1524, 1584, 2965, 3362
BspANI GGCC 4 cut(s) 347, 2106, 2837, 3264
BspCNI CTCAG 6 cut(s) 437, 772, 1237, 1746, 1945, 3126
BspHI TCATGA 3 cut(s) 751, 2446, 3294
BspLI GGNNCC 5 cut(s) 348, 567, 804, 1577, 1809
BspMAI CTGCAG 1 cut(s) 2683
BspPI GGATC 3 cut(s) 967, 1613, 1768
BspQI GCTCTTC 1 cut(s) 2781
BspT104I TTCGAA 1 cut(s) 2272
BsrBI CCGCTC 2 cut(s) 398, 2965
BsrDI GCAATG 3 cut(s) 437, 1017, 2054
BsrGI TGTACA 1 cut(s) 1192
BsrI ACTGG 7 cut(s) 454, 569, 590, 908, 2226, 2605, 2706
BssECI CCNNGG 7 cut(s) 77, 831, 1507, 1655, 1706, 2911, 2997
BssNAI GTATAC 1 cut(s) 2238
BssNI GRCGYC 2 cut(s) 26, 101
BssT1I CCWWGG 4 cut(s) 1507, 1655, 1706, 2911
Bst1107I GTATAC 1 cut(s) 2238
Bst2UI CCWGG 5 cut(s) 78, 800, 923, 1679, 3195
Bst4CI ACNGT 9 cut(s) 147, 217, 304, 996, 1073, 2123, 2416, 2923, 3307
Bst6I CTCTTC 2 cut(s) 2781, 2804
BstACI GRCGYC 2 cut(s) 26, 101
BstAPI GCANNNNNTGC 1 cut(s) 658
BstAUI TGTACA 1 cut(s) 1192
BstBI TTCGAA 1 cut(s) 2272
BstC8I GCNNGC 6 cut(s) 239, 627, 1218, 1703, 1980, 2676
BstDSI CCRYGG 1 cut(s) 831
BstENI CCTNNNNNAGG 1 cut(s) 1478
BstHHI GCGC 1 cut(s) 1851
BstMAI GTCTC 3 cut(s) 1877, 3018, 3250
BstMWI GCNNNNNNNGC 7 cut(s) 353, 605, 635, 658, 1913, 2196, 3332
BstNI CCWGG 5 cut(s) 78, 800, 923, 1679, 3195
BstNSI RCATGY 4 cut(s) 241, 1276, 2044, 2624
BstSCI CCNGG 5 cut(s) 76, 798, 921, 1677, 3193
BstSFI CTRYAG 4 cut(s) 313, 1293, 1890, 2679
BstV2I GAAGAC 2 cut(s) 1005, 2763
BstX2I RGATCY 2 cut(s) 971, 2203
BstYI RGATCY 2 cut(s) 971, 2203
BstZ17I GTATAC 1 cut(s) 2238
Bsu36I CCTNAGG 1 cut(s) 424
BsuI GTATCC 1 cut(s) 1821
BsuRI GGCC 4 cut(s) 347, 2106, 2837, 3264
BtgI CCRYGG 1 cut(s) 831
BtgZI GCGATG 1 cut(s) 1527
BtsIMutI CAGTG 9 cut(s) 213, 300, 597, 992, 1078, 2285, 2412, 2853, 3303
Cac8I GCNNGC 6 cut(s) 239, 627, 1218, 1703, 1980, 2676
CaiI CAGNNNCTG 3 cut(s) 431, 596, 1261
CciI TCATGA 3 cut(s) 751, 2446, 3294
CfoI GCGC 1 cut(s) 1851
Cfr13I GGNCC 4 cut(s) 346, 1327, 1575, 2827
CseI GACGC 3 cut(s) 15, 109, 2351
Csp6I GTAC 3 cut(s) 1193, 1438, 1923
CspCI CAANNNNNGTGG 4 cut(s) 1585, 1620, 2010, 2045
CviQI GTAC 3 cut(s) 1193, 1438, 1923
DraI TTTAAA 1 cut(s) 2781
EaeI YGGCCR 2 cut(s) 2104, 2835
Eam1104I CTCTTC 2 cut(s) 2781, 2804
EarI CTCTTC 2 cut(s) 2781, 2804
Eco130I CCWWGG 4 cut(s) 1507, 1655, 1706, 2911
Eco147I AGGCCT 1 cut(s) 3264
Eco24I GRGCYC 1 cut(s) 3114
Eco47I GGWCC 3 cut(s) 1327, 1575, 2827
Eco57I CTGAAG 2 cut(s) 150, 1485
Eco81I CCTNAGG 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 1478
EcoRI GAATTC 1 cut(s) 3198
EcoRII CCWGG 5 cut(s) 76, 798, 921, 1677, 3193
EcoT14I CCWWGG 4 cut(s) 1507, 1655, 1706, 2911
EcoT22I ATGCAT 3 cut(s) 1653, 2587, 2757
EcoT38I GRGCYC 1 cut(s) 3114
ErhI CCWWGG 4 cut(s) 1507, 1655, 1706, 2911
FalI AAGNNNNNCTT 8 cut(s) 456, 488, 997, 1029, 1690, 1722, 2544, 2576
FaqI GGGAC 1 cut(s) 1561
FauI CCCGC 1 cut(s) 595
FauNDI CATATG 1 cut(s) 2707
FbaI TGATCA 1 cut(s) 1075
FblI GTMKAC 1 cut(s) 2237
FriOI GRGCYC 1 cut(s) 3114
FspI TGCGCA 1 cut(s) 1850
GlaI GCGC 1 cut(s) 1850
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 3 cut(s) 193, 891, 1700
HaeIII GGCC 4 cut(s) 347, 2106, 2837, 3264
HapII CCGG 2 cut(s) 507, 1920
HgaI GACGC 3 cut(s) 15, 109, 2351
HhaI GCGC 1 cut(s) 1851
Hin1I GRCGYC 2 cut(s) 26, 101
Hin6I GCGC 1 cut(s) 1849
HinP1I GCGC 1 cut(s) 1849
HincII GTYRAC 2 cut(s) 172, 541
HindII GTYRAC 2 cut(s) 172, 541
HindIII AAGCTT 4 cut(s) 116, 381, 623, 2462
HpaII CCGG 2 cut(s) 507, 1920
HphI GGTGA 6 cut(s) 2044, 2264, 2982, 3073, 3298, 3362
Hpy166II GTNNAC 5 cut(s) 172, 541, 1193, 2238, 3370
Hpy8I GTNNAC 5 cut(s) 172, 541, 1193, 2238, 3370
Hpy99I CGWCG 2 cut(s) 25, 1698
HpyAV CCTTC 8 cut(s) 1078, 1798, 2029, 2364, 2968, 3089, 3124, 3248
HpyCH4III ACNGT 9 cut(s) 147, 217, 304, 996, 1073, 2123, 2416, 2923, 3307
HpyCH4IV ACGT 2 cut(s) 543, 3136
HpyF10VI GCNNNNNNNGC 7 cut(s) 353, 605, 635, 658, 1913, 2196, 3332
HpySE526I ACGT 2 cut(s) 543, 3136
Hsp92I GRCGYC 2 cut(s) 26, 101
HspAI GCGC 1 cut(s) 1849
Ksp22I TGATCA 1 cut(s) 1075
LguI GCTCTTC 1 cut(s) 2781
LmnI GCTCC 8 cut(s) 395, 605, 1213, 1346, 1449, 1607, 1813, 2962
MaeII ACGT 2 cut(s) 543, 3136
MaeIII GTNAC 9 cut(s) 592, 1039, 1268, 1303, 1387, 1855, 2117, 2799, 3118
MbiI CCGCTC 2 cut(s) 398, 2965
MfeI CAATTG 2 cut(s) 2022, 2831
MflI RGATCY 2 cut(s) 971, 2203
MhlI GDGCHC 2 cut(s) 1454, 3114
MlsI TGGCCA 2 cut(s) 2106, 2837
MluNI TGGCCA 2 cut(s) 2106, 2837
MlyI GAGTC 5 cut(s) 958, 1252, 2965, 2981, 3252
MmeI TCCRAC 2 cut(s) 318, 1990
Mox20I TGGCCA 2 cut(s) 2106, 2837
Mph1103I ATGCAT 3 cut(s) 1653, 2587, 2757
MroXI GAANNNNTTC 3 cut(s) 296, 1428, 1869
MscI TGGCCA 2 cut(s) 2106, 2837
MslI CAYNNNNRTG 6 cut(s) 488, 682, 737, 756, 1277, 3149
Msp20I TGGCCA 2 cut(s) 2106, 2837
MspA1I CMGCKG 5 cut(s) 581, 1081, 1889, 2684, 3364
MspI CCGG 2 cut(s) 507, 1920
MspR9I CCNGG 5 cut(s) 78, 800, 923, 1679, 3195
MunI CAATTG 2 cut(s) 2022, 2831
Mva1269I GAATGC 3 cut(s) 493, 2469, 2882
MvaI CCWGG 5 cut(s) 78, 800, 923, 1679, 3195
MwoI GCNNNNNNNGC 7 cut(s) 353, 605, 635, 658, 1913, 2196, 3332
NdeI CATATG 1 cut(s) 2707
NlaIV GGNNCC 5 cut(s) 348, 567, 804, 1577, 1809
NmeAIII GCCGAG 2 cut(s) 2183, 3022
NmuCI GTSAC 3 cut(s) 592, 1268, 1855
NsbI TGCGCA 1 cut(s) 1850
NsiI ATGCAT 3 cut(s) 1653, 2587, 2757
NspI RCATGY 4 cut(s) 241, 1276, 2044, 2624
NspV TTCGAA 1 cut(s) 2272
PaeI GCATGC 1 cut(s) 241
PagI TCATGA 3 cut(s) 751, 2446, 3294
PceI AGGCCT 1 cut(s) 3264
PciI ACATGT 1 cut(s) 2620
PciSI GCTCTTC 1 cut(s) 2781
PcsI WCGNNNNNNNCGW 1 cut(s) 2868
PctI GAATGC 3 cut(s) 493, 2469, 2882
PdmI GAANNNNTTC 3 cut(s) 296, 1428, 1869
PfeI GAWTC 7 cut(s) 137, 855, 1286, 1787, 2018, 2290, 3280
PfoI TCCNGGA 2 cut(s) 921, 1677
PleI GAGTC 5 cut(s) 957, 1252, 2964, 2981, 3251
PpsI GAGTC 5 cut(s) 957, 1252, 2964, 2981, 3251
PscI ACATGT 1 cut(s) 2620
PsiI TTATAA 2 cut(s) 621, 3344
Psp6I CCWGG 5 cut(s) 76, 798, 921, 1677, 3193
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 5 cut(s) 76, 798, 921, 1677, 3193
PspN4I GGNNCC 5 cut(s) 348, 567, 804, 1577, 1809
PspPI GGNCC 4 cut(s) 346, 1327, 1575, 2827
PstI CTGCAG 1 cut(s) 2683
PstNI CAGNNNCTG 3 cut(s) 431, 596, 1261
PsuI RGATCY 2 cut(s) 971, 2203
PvuII CAGCTG 3 cut(s) 581, 1889, 2684
RsaI GTAC 3 cut(s) 1194, 1439, 1924
RsaNI GTAC 3 cut(s) 1193, 1438, 1923
RseI CAYNNNNRTG 6 cut(s) 488, 682, 737, 756, 1277, 3149
SapI GCTCTTC 1 cut(s) 2781
Sau96I GGNCC 4 cut(s) 346, 1327, 1575, 2827
SchI GAGTC 5 cut(s) 958, 1252, 2965, 2981, 3252
ScrFI CCNGG 5 cut(s) 78, 800, 923, 1679, 3195
SduI GDGCHC 2 cut(s) 1454, 3114
SfcI CTRYAG 4 cut(s) 313, 1293, 1890, 2679
SfuI TTCGAA 1 cut(s) 2272
SinI GGWCC 3 cut(s) 1327, 1575, 2827
SmiI ATTTAAAT 1 cut(s) 2781
SmiMI CAYNNNNRTG 6 cut(s) 488, 682, 737, 756, 1277, 3149
SmlI CTYRAG 3 cut(s) 1433, 2365, 3167
SmoI CTYRAG 3 cut(s) 1433, 2365, 3167
SphI GCATGC 1 cut(s) 241
SseBI AGGCCT 1 cut(s) 3264
SsiI CCGC 7 cut(s) 398, 602, 1081, 1524, 1584, 2965, 3362
StuI AGGCCT 1 cut(s) 3264
StyD4I CCNGG 5 cut(s) 76, 798, 921, 1677, 3193
StyI CCWWGG 4 cut(s) 1507, 1655, 1706, 2911
SwaI ATTTAAAT 1 cut(s) 2781
TaaI ACNGT 9 cut(s) 147, 217, 304, 996, 1073, 2123, 2416, 2923, 3307
TaiI ACGT 2 cut(s) 546, 3139
TaqI TCGA 9 cut(s) 133, 369, 535, 1229, 1873, 2272, 2862, 2871, 3241
TaqII GACCGA 1 cut(s) 1563
TatI WGTACW 1 cut(s) 1192
TauI GCSGC 1 cut(s) 2968
TfiI GAWTC 7 cut(s) 137, 855, 1286, 1787, 2018, 2290, 3280
TscAI CASTG 9 cut(s) 220, 307, 597, 999, 1078, 2292, 2419, 2860, 3310
TseFI GTSAC 3 cut(s) 592, 1268, 1855
Tsp45I GTSAC 3 cut(s) 592, 1268, 1855
TspGWI ACGGA 2 cut(s) 848, 3164
TspRI CASTG 9 cut(s) 220, 307, 597, 999, 1078, 2292, 2419, 2860, 3310
VpaK11BI GGWCC 3 cut(s) 1327, 1575, 2827
XagI CCTNNNNNAGG 1 cut(s) 1478
XapI RAATTY 8 cut(s) 746, 2317, 2422, 2893, 2903, 3045, 3198, 3356
XbaI TCTAGA 2 cut(s) 50, 851
XceI RCATGY 4 cut(s) 241, 1276, 2044, 2624
XcmI CCANNNNNNNNNTGG 1 cut(s) 2218
XmiI GTMKAC 1 cut(s) 2237
XmnI GAANNNNTTC 3 cut(s) 296, 1428, 1869
Zsp2I ATGCAT 3 cut(s) 1653, 2587, 2757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.