Rw2G042660

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013571
Physical Location & Seq
Forward (+)
68998875 .. 69004248
5374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042660.1

Sequence Viewer

Length: 3369 bp
ATGTCATCAGTGTCTTTGAACAAGACAAGTCCTTCTAAAAGTAGCTCTTCTAGATCAAAACATGGGGCTCATGTGGTTTCGCAGACACCCATTGATGCAAAGCTAGATGTAGACTTCAGAGAATCTGAACGGTCTTTTGATTACTCCGCCTCAGTTGACTGTAATATCTCAAGTTCAACTAGCAATGTCCTCTCCGCCACCGTATCAGCTTACCTCCGGAATATGCAGAGAGGCCGGCTCATTCAACCATTTGGTTGCTTGATTGCTATTGATGAGCAAAGCTTCAGTGTTCTTGCCTACAGTGAAAATGGCCCAGAAATGTTGGACTTGGCCCCCCATACTGTTCCAAATATTGGGCAGCAAGAAGCTTTGACTTTTGGAACAGATGTCGGCACGCTCTTTCAGTCCCCAGGTGCTGCTGCATTGCAGAAAGCAGCTAATCATGTGGAAGTTAATCTTCTCAATCCCATATTGGTGCATTGTAAAACCTCGGGTAAGCCCTTTTATGCAATTCTGCATCGAGTTGATGTTGGCTTAGTAATAGATTTAGAACCAGTAGGTCAAGATGATGTTCCGGTCACTGCTGCGGGGGCATTGAAATCTTATAAGCTTGCTGCCAAAGCCATCTCAAGATTGCAGTCCTTGCCAAGTGGGGATATATCATTGTTATGTGATGTTATAGTGAAGGAAGTTAGTGATTTGACGGGGTATGATCGCACGATGGTGTATAAATTTCATGAAGATGAACATGGGGAAGTTGTTGCTGAGTGCCATAGACCCGACCTGGAACCTTATCTGGGTTTGCACTACCCAGCCACTGATATACCACAGGCTTCAAGATTCCTCTTTATGAAGAACAAAGTTAGGATGATATGTGATTGTTTTGCCCCTTCAGTTAAAGTAATTCAAGACAAAACATTAGCTCAGCCATTGAGTCTTTGTGGATCAGCATTGAGGTCTCCTCATGATTGTCATGCACAGTATATGGCAAACATGGGTTCTGTTGCATCTCTTGTAATGTCTGTGACAATCAATGGCGACGATGGTGAGATGGAGCATGATCAGCAGAAGGGAAGAAAATTGTGGGGTTTAGTGGTCTGCCATCACACAACTCCTAGATTTGTTCCGTTTCCTTTAAGGTACGCATGTGAGTTTTTGATGCAAGTCTTTGCTGTACAGATCCACAAAGAAGTGGAGTTGGCTGCTCAGTCAAGGGAGAAACATATAATGCAAACACAATCTCTGCTTTGTGACATGCTCCTTAGGGATGCCCCTCTAGGTATTGTTACTAAATCACCTAATGTGATGGATCTTGTTAAGTGTGATGGAGCTGCACTGTATTACAGAAAGAAAGTGTGGTTACTTGGGGTCACCCCATCCGAGGCACAAGTTGGAGATATTGCTGCTTGGCTTCTTGAATACCATAGTGAGAGCACAGGCCTAAGTACTGATAGCCTTATGGAAGCTGGCTATCCAGGTGCTTCAGATCTTGGCGATGAAGTTTGTGGAATGGCTGCGATTAGAATTACTTCAATGGACTTCCTGTTTTGGTTTCGGTCCCACACCGCAAAGGAAATCAAGTGGAGTGGTGCAAAGCATGATCCTGATGAGAAGGACGATGGAAGAAAGATGCACCCCAGGTCATCATTCAAGGCTTTTTTGGAGGTAGTTAAGCGACGAAGCATACCATGGGAAGATGTTGAAATGGATGTCATCCATTCGTTACAGCTGATATTGAGAGAATCATTGCAAGGCAATATTGTGGATGATTCCAAAATGATTGTGACTGGTCCATCAGTTGATGACAGGATGCAGAGAGTGGATGAATTGCGTGTTGCCACAACTGAAATGGTTCGGCTTATTGAGACAGCTGCAGTTCCCATTTTTGCTGTTGATGCTACTGGTAATATAAATGGGTGGAATAACAAGGCAGCTGAACTAACAGGATTGGCTGTTGAGCAAGCTATTGGTAGGCCACTAGTTGATATTGTGGGGGATGATTCAATTGAAGTGGTGAAGAACATGCTCTCATTTGCATTGCAAGGTGTTGAGAAGCAAAATGTTGAAATCAAATTGAAAACATTCGGTCATCAAGAAAATGGCAGTCTTACAATCTTGGTGGTTAATGCATGTTGTAGCCGAGATATAAAGGAAGATGTTGTTGGGGTTTGCTTTGTTGCCCAGGATCTTACAGGAGAAAAGATTGTTAAGGACAAATACACTCGTTTGCTAGGTGATTATGTTGGAATAGTGCAAACCCCTTCTGCACTGATTCCTCCAATATTTATGATTGATGAAAACTGTCATTGCTCGGAATGGAATGATGCTATGCAAAATCTGTCTGGTTTGCGAAGGGAAGAAGCTGTTGGTCGAGCACTACTTGGGGAAGTCTTCACAGTTAGCAATTTTGGCTGTCGTGTTAAAGATCATGACACATTAACCAAGCTTAGGATACTGCTGAATGGAGTACTTGCAGGCCAGGATGCTTGTAAATTGTTTTTTGGATTTTTTGATCTGCAGGGTAATTACGTTGAAGCATTACTTTCTGCTAACAAGAGAAGTGATGAAGAAGGAAGGATAACTGGGGTTTTGTGCTTTATATATGTTGCTAGTCCAGAGCTTCAGTATGCTATGCAGGTGCAAAGAATAGCAGAGCAGGCTGCAGCTGATAGTCTTAGGAAAGTGGCCTATATTCGTCAAGAAATCAAAAAGCCTCTAAGTGGGATTATGCTAATGCAAAATCTAATGGGGTCTTCTAATTTAAGCAAAGAGCAGCAGCAGCTACATAAGAAGAGCACATTGTGTCGGGAACAATTGACCAAGATTGTTGATGACACTGATGTTGAAAGTATGGAGGAGTGCTACATGGAGATGAATTCTGTTGAATTCAACCTCGGTGAAGCTATGGAAGTTGTCATAAATCAAGTTATGATCTTGAGTCGGGAGCGGCAGGTGCAGGTCATCCATGATTCACCAGCTGAGGTGTCAACCATGGTCTTGTATGGAGACAATTTGAGGCTACAACAAGTCATCTCAGATTTCTTGACAAACGCAATCCAGTTCACCCCTTCTTTTGATGGATCAACGGTTGTTCTCACAGCGATTCCAACCAAGGAACGTATAGGGACTAAAATGCACATTGTTCATGTCGAGTTTCGAATCACTCACCCAGCACCAGGAATGCCAGATTATTTGATTCAAGAGATGTTTCATGACAGCAATCGCGTCTCAAGGGAAGGGCTCGGCTTACACCTCAGCCAAAATCTGGCGAAGATCATGAATGGCAGTGTACAATATCACAGAGGAGCAGAGACATCATCCTTTAGAATCCTCATAGACTTTCCACTGGTTCACCACATTAACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1122

Amino Acids

124.34

Weight (kDa)

5.54

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 70 - 186 9.9e-35 PAS fold
GAF PF01590 220 - 394 3.7e-17 GAF domain
PHY PF00360 410 - 585 2.5e-56 Phytochrome region
PAS PF00989 616 - 729 4.9e-23 PAS fold
PAS_4 PF08448 625 - 733 1.8e-07 PAS fold
PAS PF00989 746 - 867 1.8e-13 PAS fold
HATPase_c PF02518 1002 - 1113 2.1e-12 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35840
fragaria_vesca FvH4_6g40650 FvH4_6g40650 FvH4_6g40650
malus_domestica MD09G1126400.v1.1
prunus_persica Prupe.3G198200_v2.0.a1 Prupe.3G198200_v2.0.a1
pyrus_communis pycom09g05070 pycom17g10740
rosa_chinensis RchiOBHm_Chr2g0156021
rosa_laevigata RLG00000020882
rosa_multiflora Rmu_sc0011922.1_g000002
rosa_rugosa Rorug02G0451300
rosa_samantha Rh2AG518100 Rh2BG529300 Rh2CG502800 Rh2DG538400
rosa_wichuraiana Rw2G042660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AarI CACCTGC 2 cut(s) 2626, 2941
Acc36I ACCTGC 3 cut(s) 2626, 2941, 2947
AccB7I CCANNNNNTGG 2 cut(s) 353, 3265
AccBSI CCGCTC 1 cut(s) 2947
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 3225
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 5 cut(s) 147, 195, 587, 1566, 2947
AclWI GGATC 6 cut(s) 952, 1174, 1317, 1595, 2193, 3088
AcsI RAATTY 3 cut(s) 731, 2875, 2885
AcuI CTGAAG 5 cut(s) 100, 268, 876, 1467, 2606
AdeI CACNNNGTG 1 cut(s) 2802
AfaI GTAC 5 cut(s) 1142, 1176, 1447, 2469, 3291
AfiI CCNNNNNNNGG 7 cut(s) 353, 797, 1381, 2448, 2720, 3176, 3265
AhlI ACTAGT 1 cut(s) 1978
AjnI CCWGG 7 cut(s) 409, 783, 1474, 1637, 2181, 2478, 3175
AjuI GAANNNNNNNTTGG 4 cut(s) 2145, 2177, 2349, 2381
AleI CACNNNNGTG 1 cut(s) 722
Alw21I GWGCWC 3 cut(s) 1436, 2377, 2798
Alw26I GTCTC 5 cut(s) 963, 1859, 3000, 3232, 3305
AlwI GGATC 6 cut(s) 952, 1174, 1317, 1595, 2193, 3088
AlwNI CAGNNNCTG 2 cut(s) 416, 818
Ama87I CYCGRG 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 7 cut(s) 232, 310, 330, 1438, 1973, 2476, 2685
ApoI RAATTY 3 cut(s) 731, 2875, 2885
Asp700I GAANNNNTTC 3 cut(s) 1528, 1851, 2081
AspS9I GGNCC 4 cut(s) 311, 331, 1557, 1790
AsuII TTCGAA 1 cut(s) 3157
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 2 cut(s) 1557, 1790
AxyI CCTNAGG 1 cut(s) 1262
BaeI ACNNNNGTAYC 2 cut(s) 1132, 1165
BanII GRGCYC 2 cut(s) 70, 3243
BbsI GAAGAC 2 cut(s) 2383, 2745
Bbv12I GWGCWC 3 cut(s) 1436, 2377, 2798
BbvCI CCTCAGC 2 cut(s) 2979, 3254
BciT130I CCWGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
BciVI GTATCC 1 cut(s) 2445
BclI TGATCA 1 cut(s) 1060
BcoDI GTCTC 5 cut(s) 963, 1859, 3000, 3232, 3305
BcuI ACTAGT 1 cut(s) 1978
BfaI CTAG 8 cut(s) 51, 104, 180, 1116, 1277, 1979, 2231, 2610
BfmI CTRYAG 4 cut(s) 298, 1872, 2516, 2661
BfuAI ACCTGC 3 cut(s) 2626, 2941, 2947
BfuI GTATCC 1 cut(s) 2445
BglII AGATCT 1 cut(s) 1486
BlpI GCTNAGC 1 cut(s) 924
BmcAI AGTACT 2 cut(s) 1447, 2469
Bme1390I CCNGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
Bme18I GGWCC 2 cut(s) 1557, 1790
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 4 cut(s) 311, 331, 1557, 1790
BmiI GGNNCC 3 cut(s) 333, 789, 1559
BmrFI CCNGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
BmrI ACTGGG 1 cut(s) 2592
BmuI ACTGGG 1 cut(s) 2592
BpiI GAAGAC 2 cut(s) 2383, 2745
BplI GAGNNNNNCTC 4 cut(s) 222, 254, 946, 978
Bpu10I CCTNAGC 3 cut(s) 2447, 2979, 3254
Bpu1102I GCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 3157
BpuEI CTTGAG 4 cut(s) 154, 613, 2956, 3214
BsaI GGTCTC 1 cut(s) 963
BsaJI CCNNGG 9 cut(s) 409, 489, 1380, 1637, 1688, 2181, 2893, 2991, 3111
BsaWI WCCGGW 2 cut(s) 216, 574
Bsc4I CCNNNNNNNGG 7 cut(s) 353, 797, 1381, 2448, 2720, 3176, 3265
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 6 cut(s) 554, 1792, 1906, 2587, 3058, 3351
Bse21I CCTNAGG 1 cut(s) 1262
Bse3DI GCAATG 5 cut(s) 190, 422, 1745, 2036, 2305
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
BseDI CCNNGG 9 cut(s) 409, 489, 1380, 1637, 1688, 2181, 2893, 2991, 3111
BseLI CCNNNNNNNGG 7 cut(s) 353, 797, 1381, 2448, 2720, 3176, 3265
BseMI GCAATG 5 cut(s) 190, 422, 1745, 2036, 2305
BseMII CTCAG 7 cut(s) 165, 756, 938, 1220, 2970, 3048, 3268
BseNI ACTGG 6 cut(s) 554, 1792, 1906, 2587, 3058, 3351
BseRI GAGGAG 3 cut(s) 951, 2870, 3318
BseYI CCCAGC 2 cut(s) 811, 3169
BsgI GTGCAG 3 cut(s) 1317, 2250, 2975
Bsh1236I CGCG 1 cut(s) 3225
BshFI GGCC 7 cut(s) 234, 312, 332, 1440, 1975, 2478, 2687
BsiHKAI GWGCWC 3 cut(s) 1436, 2377, 2798
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 3 cut(s) 217, 235, 575
BslFI GGGAC 3 cut(s) 391, 1543, 3139
BslI CCNNNNNNNGG 7 cut(s) 353, 797, 1381, 2448, 2720, 3176, 3265
BsmAI GTCTC 5 cut(s) 963, 1859, 3000, 3232, 3305
BsmBI CGTCTC 1 cut(s) 3232
BsmFI GGGAC 3 cut(s) 391, 1543, 3139
BsmI GAATGC 1 cut(s) 3186
BsnI GGCC 7 cut(s) 234, 312, 332, 1440, 1975, 2478, 2687
Bso31I GGTCTC 1 cut(s) 963
BsoBI CYCGRG 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 3157
Bsp1286I GDGCHC 5 cut(s) 70, 1436, 2377, 2798, 3243
Bsp13I TCCGGA 1 cut(s) 216
Bsp1407I TGTACA 2 cut(s) 1174, 3289
Bsp1720I GCTNAGC 1 cut(s) 924
Bsp19I CCATGG 2 cut(s) 1688, 2991
BspACI CCGC 5 cut(s) 147, 195, 587, 1566, 2947
BspANI GGCC 7 cut(s) 234, 312, 332, 1440, 1975, 2478, 2687
BspCNI CTCAG 7 cut(s) 164, 757, 937, 1219, 2971, 3047, 3267
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 1 cut(s) 3225
BspHI TCATGA 5 cut(s) 736, 964, 2428, 3211, 3276
BspLI GGNNCC 3 cut(s) 333, 789, 1559
BspMAI CTGCAG 3 cut(s) 1876, 2520, 2665
BspMI ACCTGC 3 cut(s) 2626, 2941, 2947
BspPI GGATC 6 cut(s) 952, 1174, 1317, 1595, 2193, 3088
BspQI GCTCTTC 2 cut(s) 52, 2786
BspT104I TTCGAA 1 cut(s) 3157
BspTNI GGTCTC 1 cut(s) 963
BsrBI CCGCTC 1 cut(s) 2947
BsrDI GCAATG 5 cut(s) 190, 422, 1745, 2036, 2305
BsrFI RCCGGY 1 cut(s) 234
BsrGI TGTACA 2 cut(s) 1174, 3289
BsrI ACTGG 6 cut(s) 554, 1792, 1906, 2587, 3058, 3351
BssAI RCCGGY 1 cut(s) 234
BssECI CCNNGG 9 cut(s) 409, 489, 1380, 1637, 1688, 2181, 2893, 2991, 3111
BssT1I CCWWGG 3 cut(s) 1688, 2991, 3111
Bst2UI CCWGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
Bst6I CTCTTC 2 cut(s) 52, 2786
BstAPI GCANNNNNTGC 1 cut(s) 643
BstAUI TGTACA 2 cut(s) 1174, 3289
BstBI TTCGAA 1 cut(s) 3157
BstC8I GCNNGC 7 cut(s) 236, 395, 612, 1468, 1962, 2476, 2658
BstDSI CCRYGG 2 cut(s) 1688, 2991
BstEII GGTNACC 1 cut(s) 1369
BstFNI CGCG 1 cut(s) 3225
BstMAI GTCTC 5 cut(s) 963, 1859, 3000, 3232, 3305
BstMWI GCNNNNNNNGC 9 cut(s) 590, 620, 643, 1063, 1895, 2409, 2657, 2779, 2953
BstNI CCWGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
BstNSI RCATGY 4 cut(s) 1149, 1258, 2026, 2133
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 7 cut(s) 409, 783, 1474, 1637, 2181, 2478, 3175
BstSFI CTRYAG 4 cut(s) 298, 1872, 2516, 2661
BstUI CGCG 1 cut(s) 3225
BstV2I GAAGAC 2 cut(s) 2383, 2745
BstX2I RGATCY 4 cut(s) 1179, 1309, 1486, 2185
BstYI RGATCY 4 cut(s) 1179, 1309, 1486, 2185
Bsu36I CCTNAGG 1 cut(s) 1262
BsuI GTATCC 1 cut(s) 2445
BsuRI GGCC 7 cut(s) 234, 312, 332, 1440, 1975, 2478, 2687
BtgI CCRYGG 2 cut(s) 1688, 2991
BtgZI GCGATG 1 cut(s) 1509
BtsI GCAGTG 2 cut(s) 579, 3292
BveI ACCTGC 3 cut(s) 2626, 2941, 2947
Cac8I GCNNGC 7 cut(s) 236, 395, 612, 1468, 1962, 2476, 2658
CaiI CAGNNNCTG 2 cut(s) 416, 818
CciI TCATGA 5 cut(s) 736, 964, 2428, 3211, 3276
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 4 cut(s) 311, 331, 1557, 1790
CseI GACGC 1 cut(s) 3214
Csp6I GTAC 5 cut(s) 1141, 1175, 1446, 2468, 3290
CspCI CAANNNNNGTGG 6 cut(s) 1567, 1602, 1992, 2027, 2100, 2135
CviQI GTAC 5 cut(s) 1141, 1175, 1446, 2468, 3290
DraIII CACNNNGTG 1 cut(s) 2802
Eam1104I CTCTTC 2 cut(s) 52, 2786
EarI CTCTTC 2 cut(s) 52, 2786
EciI GGCGGA 2 cut(s) 136, 184
Eco130I CCWWGG 3 cut(s) 1688, 2991, 3111
Eco147I AGGCCT 1 cut(s) 1440
Eco24I GRGCYC 2 cut(s) 70, 3243
Eco31I GGTCTC 1 cut(s) 963
Eco47I GGWCC 2 cut(s) 1557, 1790
Eco57I CTGAAG 5 cut(s) 100, 268, 876, 1467, 2606
Eco81I CCTNAGG 1 cut(s) 1262
Eco88I CYCGRG 1 cut(s) 490
Eco91I GGTNACC 1 cut(s) 1369
EcoO65I GGTNACC 1 cut(s) 1369
EcoRI GAATTC 2 cut(s) 2875, 2885
EcoRII CCWGG 7 cut(s) 409, 783, 1474, 1637, 2181, 2478, 3175
EcoT14I CCWWGG 3 cut(s) 1688, 2991, 3111
EcoT22I ATGCAT 1 cut(s) 2131
EcoT38I GRGCYC 2 cut(s) 70, 3243
ErhI CCWWGG 3 cut(s) 1688, 2991, 3111
Esp3I CGTCTC 1 cut(s) 3232
FalI AAGNNNNNCTT 6 cut(s) 31, 63, 441, 473, 2526, 2558
FaqI GGGAC 3 cut(s) 391, 1543, 3139
FauI CCCGC 1 cut(s) 580
FbaI TGATCA 1 cut(s) 1060
FblI GTMKAC 1 cut(s) 111
FriOI GRGCYC 2 cut(s) 70, 3243
FspBI CTAG 8 cut(s) 51, 104, 180, 1116, 1277, 1979, 2231, 2610
GsaI CCCAGC 2 cut(s) 815, 3173
HaeIII GGCC 7 cut(s) 234, 312, 332, 1440, 1975, 2478, 2687
HapII CCGG 3 cut(s) 217, 235, 575
HgaI GACGC 1 cut(s) 3214
HincII GTYRAC 2 cut(s) 157, 2988
HindII GTYRAC 2 cut(s) 157, 2988
HindIII AAGCTT 4 cut(s) 280, 366, 608, 2444
HpaII CCGG 3 cut(s) 217, 235, 575
Hpy166II GTNNAC 6 cut(s) 112, 157, 2988, 3063, 3290, 3352
Hpy188I TCNGA 6 cut(s) 119, 127, 1381, 1486, 2314, 3037
Hpy8I GTNNAC 6 cut(s) 112, 157, 2988, 3063, 3290, 3352
Hpy99I CGWCG 2 cut(s) 1043, 1680
HpyCH4IV ACGT 2 cut(s) 2529, 3118
HpyF10VI GCNNNNNNNGC 9 cut(s) 590, 620, 643, 1063, 1895, 2409, 2657, 2779, 2953
HpySE526I ACGT 2 cut(s) 2529, 3118
Kpn2I TCCGGA 1 cut(s) 216
KroI GCCGGC 1 cut(s) 234
KroNI GCCGGC 1 cut(s) 236
Ksp22I TGATCA 1 cut(s) 1060
LguI GCTCTTC 2 cut(s) 52, 2786
LmnI GCTCC 5 cut(s) 1054, 1263, 1328, 2944, 3305
MaeI CTAG 8 cut(s) 51, 104, 180, 1116, 1277, 1979, 2231, 2610
MaeII ACGT 2 cut(s) 2529, 3118
MaeIII GTNAC 8 cut(s) 577, 1024, 1250, 1285, 1359, 1369, 1722, 1783
MbiI CCGCTC 1 cut(s) 2947
MfeI CAATTG 2 cut(s) 2004, 2813
MflI RGATCY 4 cut(s) 1179, 1309, 1486, 2185
MhlI GDGCHC 5 cut(s) 70, 1436, 2377, 2798, 3243
MlyI GAGTC 2 cut(s) 943, 2947
MmeI TCCRAC 4 cut(s) 303, 1372, 2224, 3131
Mph1103I ATGCAT 1 cut(s) 2131
MroI TCCGGA 1 cut(s) 216
MroNI GCCGGC 1 cut(s) 234
MroXI GAANNNNTTC 3 cut(s) 1528, 1851, 2081
MslI CAYNNNNRTG 5 cut(s) 473, 667, 722, 741, 2870
MspA1I CMGCKG 6 cut(s) 1729, 1871, 1934, 2666, 2978, 3366
MspI CCGG 3 cut(s) 217, 235, 575
MspR9I CCNGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
MunI CAATTG 2 cut(s) 2004, 2813
Mva1269I GAATGC 1 cut(s) 3186
MvaI CCWGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
MvnI CGCG 1 cut(s) 3225
MwoI GCNNNNNNNGC 9 cut(s) 590, 620, 643, 1063, 1895, 2409, 2657, 2779, 2953
NaeI GCCGGC 1 cut(s) 236
NcoI CCATGG 2 cut(s) 1688, 2991
NgoMIV GCCGGC 1 cut(s) 234
NlaIV GGNNCC 3 cut(s) 333, 789, 1559
NmeAIII GCCGAG 2 cut(s) 2165, 3222
NmuCI GTSAC 5 cut(s) 577, 1024, 1250, 1369, 1783
NsiI ATGCAT 1 cut(s) 2131
NspI RCATGY 4 cut(s) 1149, 1258, 2026, 2133
NspV TTCGAA 1 cut(s) 3157
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 5 cut(s) 736, 964, 2428, 3211, 3276
PaqCI CACCTGC 2 cut(s) 2626, 2941
PceI AGGCCT 1 cut(s) 1440
PciSI GCTCTTC 2 cut(s) 52, 2786
PctI GAATGC 1 cut(s) 3186
PdiI GCCGGC 1 cut(s) 236
PdmI GAANNNNTTC 3 cut(s) 1528, 1851, 2081
PflMI CCANNNNNTGG 2 cut(s) 353, 3265
PleI GAGTC 2 cut(s) 942, 2946
PpsI GAGTC 2 cut(s) 942, 2946
PsiI TTATAA 1 cut(s) 606
Psp6I CCWGG 7 cut(s) 409, 783, 1474, 1637, 2181, 2478, 3175
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 2 cut(s) 811, 3169
PspGI CCWGG 7 cut(s) 409, 783, 1474, 1637, 2181, 2478, 3175
PspN4I GGNNCC 3 cut(s) 333, 789, 1559
PspPI GGNCC 4 cut(s) 311, 331, 1557, 1790
PstI CTGCAG 3 cut(s) 1876, 2520, 2665
PstNI CAGNNNCTG 2 cut(s) 416, 818
PsuI RGATCY 4 cut(s) 1179, 1309, 1486, 2185
PvuII CAGCTG 6 cut(s) 1729, 1871, 1934, 2666, 2978, 3366
RsaI GTAC 5 cut(s) 1142, 1176, 1447, 2469, 3291
RsaNI GTAC 5 cut(s) 1141, 1175, 1446, 2468, 3290
RseI CAYNNNNRTG 5 cut(s) 473, 667, 722, 741, 2870
SapI GCTCTTC 2 cut(s) 52, 2786
Sau96I GGNCC 4 cut(s) 311, 331, 1557, 1790
ScaI AGTACT 2 cut(s) 1447, 2469
SchI GAGTC 2 cut(s) 943, 2947
ScrFI CCNGG 7 cut(s) 411, 785, 1476, 1639, 2183, 2480, 3177
SduI GDGCHC 5 cut(s) 70, 1436, 2377, 2798, 3243
SfcI CTRYAG 4 cut(s) 298, 1872, 2516, 2661
SfuI TTCGAA 1 cut(s) 3157
SinI GGWCC 2 cut(s) 1557, 1790
SmiMI CAYNNNNRTG 5 cut(s) 473, 667, 722, 741, 2870
SmlI CTYRAG 4 cut(s) 169, 628, 2935, 3229
SmoI CTYRAG 4 cut(s) 169, 628, 2935, 3229
SpeI ACTAGT 1 cut(s) 1978
SseBI AGGCCT 1 cut(s) 1440
SsiI CCGC 5 cut(s) 147, 195, 587, 1566, 2947
SspI AATATT 3 cut(s) 352, 1759, 2283
SspMI CTAG 8 cut(s) 51, 104, 180, 1116, 1277, 1979, 2231, 2610
StuI AGGCCT 1 cut(s) 1440
StyD4I CCNGG 7 cut(s) 409, 783, 1474, 1637, 2181, 2478, 3175
StyI CCWWGG 3 cut(s) 1688, 2991, 3111
TaiI ACGT 2 cut(s) 2532, 3121
TaqI TCGA 4 cut(s) 520, 2371, 3150, 3157
TaqII GACCGA 2 cut(s) 1545, 2075
TatI WGTACW 4 cut(s) 1174, 1445, 2467, 3289
TauI GCSGC 1 cut(s) 2950
TseFI GTSAC 5 cut(s) 577, 1024, 1250, 1369, 1783
Tsp45I GTSAC 5 cut(s) 577, 1024, 1250, 1369, 1783
TspGWI ACGGA 1 cut(s) 1116
Van91I CCANNNNNTGG 2 cut(s) 353, 3265
VpaK11BI GGWCC 2 cut(s) 1557, 1790
XapI RAATTY 3 cut(s) 731, 2875, 2885
XbaI TCTAGA 1 cut(s) 50
XceI RCATGY 4 cut(s) 1149, 1258, 2026, 2133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1846
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 3 cut(s) 1528, 1851, 2081
XspI CTAG 8 cut(s) 51, 104, 180, 1116, 1277, 1979, 2231, 2610
ZrmI AGTACT 2 cut(s) 1447, 2469
Zsp2I ATGCAT 1 cut(s) 2131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.