Rh2BG529300

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013571
Physical Location & Seq
Forward (+)
73871592 .. 73877556
5965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG529300.1

Sequence Viewer

Length: 3162 bp
ATGTCATCAGTGTCTTTGAACAAGACAAGTCCTTCTAAAAGTAGCTCTTCTAGATCAAAACATGGGGCTCATGTGGTTTCGCAGACACCCATTGATGCAAAGCTAGATGTAGACTTCAGAGAATCTGAACGGTCTTTTGATTACTCCGCCTCAGTTGACTGTAATATCTCAAGTTCAACTAGCAATGTCCTCTCCGCCACCGTATCAGCTTACCTCCGGAATATGCAGAGAGGCCGGCTCATTCAACCATTTGGTTGCTTGATTGCTATTGATGAGCAAAGCTTCAGTGTTCTTGCCTACAGTGAAAATGCCCCAGAAATGTTGGACTTGGCCCCCCATACTGTTCCAAATATTGGGCAGCAAGAAGCTTTGACTTTTGGAACAGATGTCGGCACGCTCTTTCAGTCCCAAGGGGCTACTGCATTGCAGAAAGCAGCTAATCATGTTGAAGTTAATCTTCTCAATCCCATATTGGTGCATTGTAAAACCTCGGGTAAGCCCTTTTATGCAATTCTGCATCGAGTTGATGTTGGCTTAGTAATAGATTTAGAACCAGTAGGTCAAGATGATGTTCCGGTCACTGCTGTGGGGGCATTGAAATCTTATAAGCTTGCTGCCAAAGCCATCTCAAGATTGCAGTCCTTGCCAAGTGGGGATATATCATTGTTATGTGATGTTATAGTGAAGGAAGTTAGTGATTTGACGGGGTATGATCGCACGATGGTGTATAAATTTCATGAAGATGAACATGGGGAAGTTGTTGCTGAGTGCCATAGACCCGACCTGGAACCTTATCTGGGTTTGCACTACCCAGCCACTGATATACCACAGGCTTCAAGATTCCTTTTTATGAAGAACAAAGTTAGGATGATATGTGATTGTTTTGCCCCTTCAGTTAAAGTAATTCAAGACAAAACATTAGCTCAGCCATTGAGTCTTTGTGGATCAGCATTGAGGTCTCCTCATGATTGTCATGCACAGTATATGGCAAACATGGGTTCTGTTGCATCTCTTGTAATGTCTGTGACAATCAATGGCGACGATGGTGAGATGGAGCATGATCAGCAGAAGGGAAGAAAATTGTGGGGTTTAGTGGTCTGCCATCACACAACTCCTAGATTTGTTCCGTTTCCTTTAAGGTACGCATGTGAGTTTTTGATGCAAGTCTTTGCTGTACAGATCCACAAAGAAGTGGAGTTGGCTGCCCAGTCAAGGGAGAAACATATAATGCAAACACAATCTCTGCTTTGTGACATGCTCCTTAGGGATGCCCCTCTAGGTATTGTTACTCAATCACCTAATGTGATGGATCTTGTTAAGTGTGATGGAGCTGCACTGTATTACAGAAAGAAAGTGTGGTTACTTGGGGTCACCCCATCCGAGGCACAAGTTGGAGATATTGCTGCATGGCTTCTTGAATACCATAGTGAGAGCACAGGCCTAAGTACTGATAGCCTTATGGAAGCTGGCTATCCAGGTGCTTCAGATCTTGGTGATGAAGTTTGTGGAATGGCTGCGATTAGAATTACTTCAATGGACTTCCTGTTTTGGTTTCGGTCCCACACTGCAAAGGAAATCAAGTGGAGCGGTGCAAAGCATGATCCTGATGAGAAGGACGATGGAAGAAAGATGCACCCCAGGTCATCATTCAAGGCTTTTTTGGAGGTAGTTAAGCGACGAAGCATACCATGGGAAGATGTTGAAATGGATGTCATCCATTCTTTACAGCTGATATTGAGAGAATCATTGCAAGACAATATTGTAGATGATTCCAAAATGATTGTGACTGGTCCATCAGTTGATGACAGGATGCAGAGAGTGGATGAATTGCGTGTTGCCACAACTGAAATGGTTCGGCTTATTGAGACAGCTGCAGTTCCCATTTTTGCTGTTGATGCTACTGGTAATATAAATGGGTGGAATAACAAGGCAGCTGAACTAACAGGATTGGCTGTTGAGCAAGCTATTGGTAGGCCGTTAGTTGATATTGTGGGGGATGATTCAATTGAAGTGGTGAAGAACATGCTCTCATTTGCATTGCAAGGTGTTGAGAAGCAAAATGTTGAAATCAAATTGAAAACATTCGGTCATCAAGAAAATGGCAGTCTTACAATCTTGGTGGTTAATGCATGTTGTAGCCGAGATATAAAGGAAGATGTTGTTGGGGTTTGCTTTGTTGCCCAGGATCTTACAGGAGAAAAGATTGTTAAGGACAAATACACTCGTTTGCTAGGTGATTATGTTGGAATAGTGCAAACCCCTTCTGCACTGATTCCTCCAATATTTATGATTGATGAAAACTGTCATTGCTCGGAATGGAATGATGCTATGCAAAATCTGTCTGGTTTGCGAAGGGAAGAAGCTGTTGGTCAAGCACTACTTGGGGAAGTCTTCACAGTTAGCAATTTTGGCTGTCGTGTTAAAGATCATGACACATTAACCAAGCTTAGGATACTGCTGAATGGAGTACTTGCAGGCCAGGATGCTTGTAAATTGTTTTTTGGATTTTTTGATCTGCAGGGTAATTACGTTGAAGCATTACTTTCTGCTAACAAGAGAAGTGATGAAGAAGGAAGGATAACTGGGGTTTTGTGCTTTATATATGTTGCTAGTCCAGAGCTTCAGTATGCTATGCAGGTGCAAAGAATAGCAGAGCAGGCTGCAGCTGATAGTCTTAGGAAAGTGGCCTATATTCGTCAAGAAATCAAAAAGCCTCTAAGTGGGATTATGCTAATGCAAAATCTAATGGGGTCTTCTAATTTAAGCAAAGAGCAGCAGCAGCTACATAAGAAGAGCACATTGTGTCGGGAACAATTGACCAAGATTGTTGATGACACTGATGTTGAAAGTATGGAGGAGTGCTACATGGAGATGAATTCTGTTGAATTCAACCTCGGTGAAGCTCTGGAAGTTGTCATAAATCAAGTTATGATCTTGAGTCGGGAGCGGCAGGTGCAGGTCATCCATGATTCACCAGCTGAGGTGTCAACCATGGTCTTGTATGGAGACAATTTGAGGCTACAACAAGTCATCTCAGATTTCTTGACAAACGCAATCCAGTTCACCCCGGCTTTTGATGGATCAACGGTTGTTCTAACAGCGATTCCAACCAAGGAACGTGTAGGGACTAAAATGCACATTGTTCATGTCGAGTTTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1053

Amino Acids

116.64

Weight (kDa)

5.33

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 70 - 186 5.5e-36 PAS fold
GAF PF01590 220 - 394 3.3e-17 GAF domain
PHY PF00360 410 - 585 1.1e-56 Phytochrome region
PAS PF00989 616 - 729 4.5e-23 PAS fold
PAS_4 PF08448 625 - 733 1.7e-07 PAS fold
PAS PF00989 746 - 867 9.7e-14 PAS fold
HisKA PF00512 895 - 952 9.8e-06 His Kinase A (phospho-acceptor) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35840
fragaria_vesca FvH4_6g40650 FvH4_6g40650 FvH4_6g40650
malus_domestica MD09G1126400.v1.1
prunus_persica Prupe.3G198200_v2.0.a1 Prupe.3G198200_v2.0.a1
pyrus_communis pycom09g05070 pycom17g10740
rosa_chinensis RchiOBHm_Chr2g0156021
rosa_laevigata RLG00000020882
rosa_multiflora Rmu_sc0011922.1_g000002
rosa_rugosa Rorug02G0451300
rosa_samantha Rh2AG518100 Rh2BG529300 Rh2CG502800 Rh2DG538400
rosa_wichuraiana Rw2G042660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AarI CACCTGC 2 cut(s) 2626, 2941
Acc36I ACCTGC 3 cut(s) 2626, 2941, 2947
AccB7I CCANNNNNTGG 1 cut(s) 353
AccBSI CCGCTC 2 cut(s) 1587, 2947
AccI GTMKAC 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 4 cut(s) 147, 195, 1587, 2947
AclWI GGATC 6 cut(s) 952, 1174, 1317, 1595, 2193, 3088
AcsI RAATTY 3 cut(s) 731, 2875, 2885
AcuI CTGAAG 5 cut(s) 100, 268, 876, 1467, 2606
AdeI CACNNNGTG 1 cut(s) 2802
AfaI GTAC 4 cut(s) 1142, 1176, 1447, 2469
AfiI CCNNNNNNNGG 7 cut(s) 353, 797, 1212, 1213, 1381, 2448, 2720
AflIII ACRYGT 1 cut(s) 3118
AjnI CCWGG 5 cut(s) 783, 1474, 1637, 2181, 2478
AjuI GAANNNNNNNTTGG 4 cut(s) 2145, 2177, 2349, 2381
AleI CACNNNNGTG 2 cut(s) 584, 722
Alw21I GWGCWC 2 cut(s) 1436, 2798
Alw26I GTCTC 3 cut(s) 963, 1859, 3000
AlwI GGATC 6 cut(s) 952, 1174, 1317, 1595, 2193, 3088
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 6 cut(s) 232, 330, 1438, 1973, 2476, 2685
ApoI RAATTY 3 cut(s) 731, 2875, 2885
Asp700I GAANNNNTTC 3 cut(s) 1528, 1851, 2081
AspS9I GGNCC 3 cut(s) 331, 1557, 1790
AsuC2I CCSGG 1 cut(s) 3068
AsuHPI GGTGA 9 cut(s) 1058, 1287, 1363, 1505, 2026, 2246, 2909, 2964, 3055
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 2 cut(s) 1557, 1790
AxyI CCTNAGG 1 cut(s) 1262
BaeI ACNNNNGTAYC 2 cut(s) 1132, 1165
BanII GRGCYC 1 cut(s) 70
BbsI GAAGAC 2 cut(s) 2383, 2745
Bbv12I GWGCWC 2 cut(s) 1436, 2798
BbvCI CCTCAGC 1 cut(s) 2979
BceAI ACGGC 1 cut(s) 1960
BciT130I CCWGG 5 cut(s) 785, 1476, 1639, 2183, 2480
BciVI GTATCC 1 cut(s) 2445
BclI TGATCA 1 cut(s) 1060
BcnI CCSGG 1 cut(s) 3068
BcoDI GTCTC 3 cut(s) 963, 1859, 3000
BfaI CTAG 7 cut(s) 51, 104, 180, 1116, 1277, 2231, 2610
BfmI CTRYAG 4 cut(s) 298, 1872, 2516, 2661
BfuAI ACCTGC 3 cut(s) 2626, 2941, 2947
BfuI GTATCC 1 cut(s) 2445
BglII AGATCT 1 cut(s) 1486
BlpI GCTNAGC 1 cut(s) 924
BmcAI AGTACT 2 cut(s) 1447, 2469
Bme1390I CCNGG 6 cut(s) 785, 1476, 1639, 2183, 2480, 3068
Bme18I GGWCC 2 cut(s) 1557, 1790
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 3 cut(s) 331, 1557, 1790
BmiI GGNNCC 3 cut(s) 333, 789, 1559
BmrFI CCNGG 6 cut(s) 785, 1476, 1639, 2183, 2480, 3068
BmrI ACTGGG 2 cut(s) 1201, 2592
BmuI ACTGGG 2 cut(s) 1201, 2592
BpiI GAAGAC 2 cut(s) 2383, 2745
BplI GAGNNNNNCTC 4 cut(s) 222, 254, 946, 978
Bpu10I CCTNAGC 2 cut(s) 2447, 2979
Bpu1102I GCTNAGC 1 cut(s) 924
BpuEI CTTGAG 3 cut(s) 154, 613, 2956
BpuMI CCSGG 1 cut(s) 3068
BsaI GGTCTC 1 cut(s) 963
BsaWI WCCGGW 2 cut(s) 216, 574
Bsc4I CCNNNNNNNGG 7 cut(s) 353, 797, 1212, 1213, 1381, 2448, 2720
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 6 cut(s) 554, 1207, 1792, 1906, 2587, 3058
Bse21I CCTNAGG 1 cut(s) 1262
Bse3DI GCAATG 5 cut(s) 190, 422, 1745, 2036, 2305
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 5 cut(s) 785, 1476, 1639, 2183, 2480
BseLI CCNNNNNNNGG 7 cut(s) 353, 797, 1212, 1213, 1381, 2448, 2720
BseMI GCAATG 5 cut(s) 190, 422, 1745, 2036, 2305
BseMII CTCAG 5 cut(s) 165, 756, 938, 2970, 3048
BseNI ACTGG 6 cut(s) 554, 1207, 1792, 1906, 2587, 3058
BseRI GAGGAG 2 cut(s) 951, 2870
BseYI CCCAGC 1 cut(s) 811
BsgI GTGCAG 3 cut(s) 1317, 2250, 2975
BshFI GGCC 6 cut(s) 234, 332, 1440, 1975, 2478, 2687
BsiHKAI GWGCWC 2 cut(s) 1436, 2798
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 4 cut(s) 217, 235, 575, 3068
BslFI GGGAC 3 cut(s) 391, 1543, 3139
BslI CCNNNNNNNGG 7 cut(s) 353, 797, 1212, 1213, 1381, 2448, 2720
BsmAI GTCTC 3 cut(s) 963, 1859, 3000
BsmFI GGGAC 3 cut(s) 391, 1543, 3139
BsnI GGCC 6 cut(s) 234, 332, 1440, 1975, 2478, 2687
Bso31I GGTCTC 1 cut(s) 963
BsoBI CYCGRG 1 cut(s) 490
Bsp1286I GDGCHC 3 cut(s) 70, 1436, 2798
Bsp13I TCCGGA 1 cut(s) 216
Bsp1407I TGTACA 1 cut(s) 1174
Bsp1720I GCTNAGC 1 cut(s) 924
Bsp19I CCATGG 2 cut(s) 1688, 2991
BspACI CCGC 4 cut(s) 147, 195, 1587, 2947
BspANI GGCC 6 cut(s) 234, 332, 1440, 1975, 2478, 2687
BspCNI CTCAG 5 cut(s) 164, 757, 937, 2971, 3047
BspEI TCCGGA 1 cut(s) 216
BspHI TCATGA 3 cut(s) 736, 964, 2428
BspLI GGNNCC 3 cut(s) 333, 789, 1559
BspMAI CTGCAG 3 cut(s) 1876, 2520, 2665
BspMI ACCTGC 3 cut(s) 2626, 2941, 2947
BspPI GGATC 6 cut(s) 952, 1174, 1317, 1595, 2193, 3088
BspQI GCTCTTC 2 cut(s) 52, 2786
BspTNI GGTCTC 1 cut(s) 963
BsrBI CCGCTC 2 cut(s) 1587, 2947
BsrDI GCAATG 5 cut(s) 190, 422, 1745, 2036, 2305
BsrFI RCCGGY 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 1174
BsrI ACTGG 6 cut(s) 554, 1207, 1792, 1906, 2587, 3058
BssAI RCCGGY 1 cut(s) 234
BssT1I CCWWGG 4 cut(s) 409, 1688, 2991, 3111
Bst2UI CCWGG 5 cut(s) 785, 1476, 1639, 2183, 2480
Bst6I CTCTTC 2 cut(s) 52, 2786
BstAPI GCANNNNNTGC 1 cut(s) 643
BstAUI TGTACA 1 cut(s) 1174
BstC8I GCNNGC 7 cut(s) 236, 395, 612, 1468, 1962, 2476, 2658
BstDSI CCRYGG 2 cut(s) 1688, 2991
BstEII GGTNACC 1 cut(s) 1369
BstMAI GTCTC 3 cut(s) 963, 1859, 3000
BstMWI GCNNNNNNNGC 9 cut(s) 590, 620, 643, 1063, 1895, 2409, 2657, 2779, 2953
BstNI CCWGG 5 cut(s) 785, 1476, 1639, 2183, 2480
BstNSI RCATGY 4 cut(s) 1149, 1258, 2026, 2133
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 6 cut(s) 783, 1474, 1637, 2181, 2478, 3066
BstSFI CTRYAG 4 cut(s) 298, 1872, 2516, 2661
BstV2I GAAGAC 2 cut(s) 2383, 2745
BstX2I RGATCY 4 cut(s) 1179, 1309, 1486, 2185
BstYI RGATCY 4 cut(s) 1179, 1309, 1486, 2185
Bsu36I CCTNAGG 1 cut(s) 1262
BsuI GTATCC 1 cut(s) 2445
BsuRI GGCC 6 cut(s) 234, 332, 1440, 1975, 2478, 2687
BtgI CCRYGG 2 cut(s) 1688, 2991
BtsI GCAGTG 2 cut(s) 579, 1563
BtsIMutI CAGTG 9 cut(s) 15, 292, 307, 579, 816, 1334, 1563, 2267, 2835
BveI ACCTGC 3 cut(s) 2626, 2941, 2947
Cac8I GCNNGC 7 cut(s) 236, 395, 612, 1468, 1962, 2476, 2658
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 3 cut(s) 736, 964, 2428
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 3 cut(s) 331, 1557, 1790
Csp6I GTAC 4 cut(s) 1141, 1175, 1446, 2468
CspCI CAANNNNNGTGG 4 cut(s) 1992, 2027, 2100, 2135
CviQI GTAC 4 cut(s) 1141, 1175, 1446, 2468
DraIII CACNNNGTG 1 cut(s) 2802
Eam1104I CTCTTC 2 cut(s) 52, 2786
EarI CTCTTC 2 cut(s) 52, 2786
EciI GGCGGA 2 cut(s) 136, 184
Eco130I CCWWGG 4 cut(s) 409, 1688, 2991, 3111
Eco147I AGGCCT 1 cut(s) 1440
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 963
Eco47I GGWCC 2 cut(s) 1557, 1790
Eco57I CTGAAG 5 cut(s) 100, 268, 876, 1467, 2606
Eco81I CCTNAGG 1 cut(s) 1262
Eco88I CYCGRG 1 cut(s) 490
Eco91I GGTNACC 1 cut(s) 1369
EcoO65I GGTNACC 1 cut(s) 1369
EcoRI GAATTC 2 cut(s) 2875, 2885
EcoRII CCWGG 5 cut(s) 783, 1474, 1637, 2181, 2478
EcoT14I CCWWGG 4 cut(s) 409, 1688, 2991, 3111
EcoT22I ATGCAT 1 cut(s) 2131
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 4 cut(s) 409, 1688, 2991, 3111
FalI AAGNNNNNCTT 8 cut(s) 31, 63, 441, 473, 2364, 2396, 2526, 2558
FaqI GGGAC 3 cut(s) 391, 1543, 3139
FbaI TGATCA 1 cut(s) 1060
FblI GTMKAC 1 cut(s) 111
FriOI GRGCYC 1 cut(s) 70
FspBI CTAG 7 cut(s) 51, 104, 180, 1116, 1277, 2231, 2610
GsaI CCCAGC 1 cut(s) 815
HaeIII GGCC 6 cut(s) 234, 332, 1440, 1975, 2478, 2687
HapII CCGG 4 cut(s) 217, 235, 575, 3068
HincII GTYRAC 2 cut(s) 157, 2988
HindII GTYRAC 2 cut(s) 157, 2988
HindIII AAGCTT 4 cut(s) 280, 366, 608, 2444
HpaII CCGG 4 cut(s) 217, 235, 575, 3068
HphI GGTGA 9 cut(s) 1058, 1287, 1363, 1505, 2026, 2246, 2909, 2964, 3055
Hpy166II GTNNAC 4 cut(s) 112, 157, 2988, 3063
Hpy188I TCNGA 6 cut(s) 119, 127, 1381, 1486, 2314, 3037
Hpy8I GTNNAC 4 cut(s) 112, 157, 2988, 3063
Hpy99I CGWCG 2 cut(s) 1043, 1680
HpyAV CCTTC 9 cut(s) 42, 679, 900, 1063, 1606, 2271, 2346, 2564, 2568
HpyCH4IV ACGT 2 cut(s) 2529, 3118
HpyF10VI GCNNNNNNNGC 9 cut(s) 590, 620, 643, 1063, 1895, 2409, 2657, 2779, 2953
HpySE526I ACGT 2 cut(s) 2529, 3118
Kpn2I TCCGGA 1 cut(s) 216
KroI GCCGGC 1 cut(s) 234
KroNI GCCGGC 1 cut(s) 236
Ksp22I TGATCA 1 cut(s) 1060
LguI GCTCTTC 2 cut(s) 52, 2786
LmnI GCTCC 5 cut(s) 1054, 1263, 1328, 1584, 2944
MaeI CTAG 7 cut(s) 51, 104, 180, 1116, 1277, 2231, 2610
MaeII ACGT 2 cut(s) 2529, 3118
MaeIII GTNAC 7 cut(s) 577, 1024, 1250, 1285, 1359, 1369, 1783
MbiI CCGCTC 2 cut(s) 1587, 2947
MfeI CAATTG 2 cut(s) 2004, 2813
MflI RGATCY 4 cut(s) 1179, 1309, 1486, 2185
MhlI GDGCHC 3 cut(s) 70, 1436, 2798
MlyI GAGTC 2 cut(s) 943, 2947
MmeI TCCRAC 4 cut(s) 303, 1372, 2224, 3131
Mph1103I ATGCAT 1 cut(s) 2131
MroI TCCGGA 1 cut(s) 216
MroNI GCCGGC 1 cut(s) 234
MroXI GAANNNNTTC 3 cut(s) 1528, 1851, 2081
MslI CAYNNNNRTG 6 cut(s) 473, 584, 667, 722, 741, 2870
MspA1I CMGCKG 5 cut(s) 1729, 1871, 1934, 2666, 2978
MspI CCGG 4 cut(s) 217, 235, 575, 3068
MspR9I CCNGG 6 cut(s) 785, 1476, 1639, 2183, 2480, 3068
MunI CAATTG 2 cut(s) 2004, 2813
MvaI CCWGG 5 cut(s) 785, 1476, 1639, 2183, 2480
MwoI GCNNNNNNNGC 9 cut(s) 590, 620, 643, 1063, 1895, 2409, 2657, 2779, 2953
NaeI GCCGGC 1 cut(s) 236
NciI CCSGG 1 cut(s) 3068
NcoI CCATGG 2 cut(s) 1688, 2991
NgoMIV GCCGGC 1 cut(s) 234
NlaIV GGNNCC 3 cut(s) 333, 789, 1559
NmeAIII GCCGAG 1 cut(s) 2165
NmuCI GTSAC 5 cut(s) 577, 1024, 1250, 1369, 1783
NsiI ATGCAT 1 cut(s) 2131
NspI RCATGY 4 cut(s) 1149, 1258, 2026, 2133
OliI CACNNNNGTG 2 cut(s) 584, 722
PagI TCATGA 3 cut(s) 736, 964, 2428
PaqCI CACCTGC 2 cut(s) 2626, 2941
PceI AGGCCT 1 cut(s) 1440
PciSI GCTCTTC 2 cut(s) 52, 2786
PdiI GCCGGC 1 cut(s) 236
PdmI GAANNNNTTC 3 cut(s) 1528, 1851, 2081
PfeI GAWTC 8 cut(s) 122, 840, 1742, 1769, 2000, 2272, 2969, 3103
PflMI CCANNNNNTGG 1 cut(s) 353
PleI GAGTC 2 cut(s) 942, 2946
PpsI GAGTC 2 cut(s) 942, 2946
PsiI TTATAA 1 cut(s) 606
Psp6I CCWGG 5 cut(s) 783, 1474, 1637, 2181, 2478
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 1 cut(s) 811
PspGI CCWGG 5 cut(s) 783, 1474, 1637, 2181, 2478
PspN4I GGNNCC 3 cut(s) 333, 789, 1559
PspPI GGNCC 3 cut(s) 331, 1557, 1790
PstI CTGCAG 3 cut(s) 1876, 2520, 2665
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 4 cut(s) 1179, 1309, 1486, 2185
PvuII CAGCTG 5 cut(s) 1729, 1871, 1934, 2666, 2978
RsaI GTAC 4 cut(s) 1142, 1176, 1447, 2469
RsaNI GTAC 4 cut(s) 1141, 1175, 1446, 2468
RseI CAYNNNNRTG 6 cut(s) 473, 584, 667, 722, 741, 2870
SapI GCTCTTC 2 cut(s) 52, 2786
Sau96I GGNCC 3 cut(s) 331, 1557, 1790
ScaI AGTACT 2 cut(s) 1447, 2469
SchI GAGTC 2 cut(s) 943, 2947
ScrFI CCNGG 6 cut(s) 785, 1476, 1639, 2183, 2480, 3068
SduI GDGCHC 3 cut(s) 70, 1436, 2798
SfcI CTRYAG 4 cut(s) 298, 1872, 2516, 2661
SinI GGWCC 2 cut(s) 1557, 1790
SmiMI CAYNNNNRTG 6 cut(s) 473, 584, 667, 722, 741, 2870
SmlI CTYRAG 3 cut(s) 169, 628, 2935
SmoI CTYRAG 3 cut(s) 169, 628, 2935
SseBI AGGCCT 1 cut(s) 1440
SsiI CCGC 4 cut(s) 147, 195, 1587, 2947
SspI AATATT 3 cut(s) 352, 1759, 2283
SspMI CTAG 7 cut(s) 51, 104, 180, 1116, 1277, 2231, 2610
StuI AGGCCT 1 cut(s) 1440
StyD4I CCNGG 6 cut(s) 783, 1474, 1637, 2181, 2478, 3066
StyI CCWWGG 4 cut(s) 409, 1688, 2991, 3111
TaiI ACGT 2 cut(s) 2532, 3121
TaqI TCGA 2 cut(s) 520, 3150
TaqII GACCGA 2 cut(s) 1545, 2075
TatI WGTACW 3 cut(s) 1174, 1445, 2467
TauI GCSGC 1 cut(s) 2950
TfiI GAWTC 8 cut(s) 122, 840, 1742, 1769, 2000, 2272, 2969, 3103
TscAI CASTG 9 cut(s) 15, 292, 307, 586, 823, 1341, 1570, 2274, 2842
TseFI GTSAC 5 cut(s) 577, 1024, 1250, 1369, 1783
Tsp45I GTSAC 5 cut(s) 577, 1024, 1250, 1369, 1783
TspGWI ACGGA 1 cut(s) 1116
TspRI CASTG 9 cut(s) 15, 292, 307, 586, 823, 1341, 1570, 2274, 2842
Van91I CCANNNNNTGG 1 cut(s) 353
VpaK11BI GGWCC 2 cut(s) 1557, 1790
XapI RAATTY 3 cut(s) 731, 2875, 2885
XbaI TCTAGA 1 cut(s) 50
XceI RCATGY 4 cut(s) 1149, 1258, 2026, 2133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1846
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 3 cut(s) 1528, 1851, 2081
XspI CTAG 7 cut(s) 51, 104, 180, 1116, 1277, 2231, 2610
ZrmI AGTACT 2 cut(s) 1447, 2469
Zsp2I ATGCAT 1 cut(s) 2131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.