Rh2AG518100

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013571
Physical Location & Seq
Forward (+)
74462475 .. 74468586
6112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG518100.1

Sequence Viewer

Length: 3378 bp
ATGTCATCAGTGTCTTTGAACAAGACAAGTCCTTCTAGAAGTAGCTCTTCTAGATCAAAACATGGGGCTCATGTGGTTTCGCAGACTCCCATTGATGCAAAGCTAGATGTAGACTTCAGAGAATCTGAACGGTCGTTTGATTACTCCGCCTCAGTTGACTGTAACATCTCAAGTTCAACTAGCAATGTCCCCTCCGCCGCTGTATCGGCTTACCTCCGGAATATGCAGAGAGGCCGGCTCATTCAGCCATTTGGTTGCTTGATTGCTATTGATGAGCAAAACTTCAGTGTTCTTGCCTACAGTGAAAATGCCCCAGAAATGTTGGACTTGGCCCCCCATACTGTTCCAAATATTGGGCAGCAAGAAGCTTTGACTTTTGGAACAGATGTTCGCACGCTCTTTCAGTCCCCAGGTGCTGCTGCATTGCAGAAAGCAGCTAATCATGGGGAAGTCAATCTTCTCAATCCCATATTGGTGCATTGTAAAACCTCGGGTAAGCCCTTTTATGCAATTCTGCATCGAGTTGATGTTGGCTTAGTAATAGATTTAGAACCAGTAGGTCAAGATGATGTTCCGGTCACTGCTGCGGGGGCATTGAAATCTTATAAGCTTGCAGCCAAAGCCATCTCAAGATTGCAGTCCTTGCCGAGTGGGGATATATCATTGTTATGTGATGTTATAGTGAAGGAAGTTAGTCATTTGACGGGGTATGATCGCACGATGGTGTATAAATTTCATGAAGATGAACATGGGGAAGTTGTTGCTGAGTGCCATAGACCTGACCTGGAACCTTATCTGGGTTTGCACTACCCAGCCACTGATATACCACAGGCTTCAAGATTCCTCTTTATGAAGAACAAAGTTAGGATGATATGTGATTGTTTTGCCCCTTCAGTTAAAGTAATTCAAGACAAAACATTAGCTCAGCCATTGAGTCTTTGTGGATCAGCATTGAGGTCTCCTCATGATTGTCATGCACAGTATATGGCAAACATGGGTTCTGTTGCATCTCTTGTAATGTCTGTGACAATCAATGGTGACGATAGTGACGATGGTGAGATGGAGCATGATCAGCAGAAGGGAAGAAAATTGTGGGGTTTAGTGGTCTGCCATCACACAACTCCTAGATTTGTTCCGTTTCCTTTAAGGTACGCATGTGAGTTTTTGATGCAAGTCTTTGCTGTACAGATCCACAAAGAAGTGGAGTTGGCTGCTCAGTCAAGGGAGAAACATATAATGCAAACACAATCTCTGCTTTGTGACATGCTCCTTAGGGATGCCCCTCTAGGTATTGTTACTCAATCACCTAATGTGATGGATCTTGTTAAGTGTGATGGAGCTGCACTGTATTACAGAAAGAAAGTGTGGTTACTTGGGGTCACCCCATCCGAGGCACAAGTTGGAGATATTGCTGCATGGCTTCTTGAATACCATAGTGAGAGCACAGGCCTAAGTACTGATAGCCTTATGGAAGCTGGCTATCCAGGTGCTTCAGATCTTGGCGATGAAGTTTGTGGAATGGCTGCGATTAGAATTACTTCAATGGACTTCCTGTTTTGGTTTCGGTCCCACACTGCAAAGGAAATCAAGTGGAGCGGTGCAAAGCATGATCCTGATGAGAAGGACGATGGAAGAAAGATGCACCCCAGGTCATCATTCAAGGCTTTTTTGGAGGTAGTTAAGCGACGAAGCATACCATGGGAAGATGTTGAAATGGATGTCATCCATTCTTTACAGCTGATATTGAGAGAATCATTGCAAGACAATATTGTGGATGATTCCAAAATGATTGTGACTGGTCCATCAGTTGATGACAGGATGCAGAGAGTGGATGAATTGCGTGTTGCCACAACTGAAATGGTTCGGCTTATTGAGACAGCTGCAGTTCCCATTTTTGCTGTTGATGCTACTGGTAATATAAATGGGTGGAATAACAAGGCAGCTGAACTAACAGGATTGGCTGTTGAGCAAGCTATTGGTAGGCCATTAGTTGATATTGTGGGGGATGATTCAATTGAAGTGGTGAAGAACATGCTCACATTTGCATTGCAAGGCGTCGAGAGGCAAAATGTTGAAATCAAATTGAAAACATTTGGTCATCAAGAAAATGGCAGTCTTACAATCTTGGTGGTTAATGCATGTTGTAGCCGAGATATAAAGGAAGATGTTGTTGGGGTTTGCTTTGTTGCCCAAGATCTTACAAGAGAAAAGATTGTTAAGGACAAATACACTCGTTTGCTAGGTGATTATGTTGGAATAGTGCAAACACCTTCTGCACTGATTCCTCCAATATTTATGACCGATGAAAACTGTCATTGCTCGGAATGGAATGATGCTATGCAAAATTTGTCTGGTTTGCGAAGGGAAGAAGCTGTTGGTCGAGCGCTCCTTGGGGAAGTCTTCACAGTTAGCAATTTTGGCTGTCGTGTTAAAGATCATGACACATTAACCAAGCTTAGGATACTGCTGAATGGAGTACTTGCAGGCCAGGATGCTTGTAAATTGTTTTTCGGATTTTTTGATCGGCAGGGTAATTACGTTGAAGCATTACTTTCTGCTAACAAGAGAAGTGATGAAGAAGGAAGGATAACTGGGGTATTGTGCTTTATATATGTTGCTAGTCCAGAGCTTCAGTATGCTATGCAGGTGCAAAGAATAGCAGAGCAGGCTGCAGCTGATAGTCTTAGGAAAGTGGCCTATATTCGTCAAGAAATCAAAAAGCCTCTAAGTGGGATTATGCTAATGCAAAATCTAATGGGGTCTTCTAATTTAAGCAAAGAGCAGCAGCAGCTACATAAGAAGAGCACATTGTGTCGGGAACAATTGACCAAGATTGTTGATGACACTGATGTTGAAAGTATGGAGGAGTGCTACATGGAGATGAATTCTGTTGAATTCAACCTCGGTGAAGCTATGGAAGTGGTCATAAATCAAGTTATGATCTTGAGTCGGGAGCGGCAGGTGCAGGTCATGCATGATTCACCAGCAGAGGTGTCAACCATGGTCTTGTATGGAGACAATTTGAGGCTACAACAAGTCATCTCAGATTTCTTGACAAACGCAATCCAGTTCACCCCTTCTTTTGATGGATCAACGGTTGTTCTCACAGCGATTCCAACCAAGGAACGTGTAGGGACTAAAATGCACATTGTTCATGTCGAGTTTCGAATCACTCACCCAGCACCAGGAATGCCAGATTATTTGATTCAAGAGATGTTTCATGACAGCAATCGCGTCTCAAGGGAAGGGCTCGGCTTACACCTCAGCCAAAATCTGGTGAAGATCATGAATGGCAGTGTACAATATCACAGAGGAGCAGAGACATCATCCTTTAGGATCCTCATAGACTTTCCACTGGTTCACCACATTAACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1125

Amino Acids

125.02

Weight (kDa)

5.57

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 70 - 186 1.8e-36 PAS fold
GAF PF01590 220 - 397 4.5e-17 GAF domain
PHY PF00360 413 - 588 1.2e-56 Phytochrome region
PAS PF00989 619 - 732 1.5e-23 PAS fold
PAS_4 PF08448 628 - 737 2.6e-08 PAS fold
PAS PF00989 749 - 870 2.1e-14 PAS fold
HATPase_c PF02518 1005 - 1116 1.1e-12 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35840
fragaria_vesca FvH4_6g40650 FvH4_6g40650 FvH4_6g40650
malus_domestica MD09G1126400.v1.1
prunus_persica Prupe.3G198200_v2.0.a1 Prupe.3G198200_v2.0.a1
pyrus_communis pycom09g05070 pycom17g10740
rosa_chinensis RchiOBHm_Chr2g0156021
rosa_laevigata RLG00000020882
rosa_multiflora Rmu_sc0011922.1_g000002
rosa_rugosa Rorug02G0451300
rosa_samantha Rh2AG518100 Rh2BG529300 Rh2CG502800 Rh2DG538400
rosa_wichuraiana Rw2G042660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AarI CACCTGC 2 cut(s) 2635, 2950
Acc36I ACCTGC 3 cut(s) 2635, 2950, 2956
AccB7I CCANNNNNTGG 2 cut(s) 353, 3274
AccBSI CCGCTC 2 cut(s) 1596, 2956
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 3234
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 6 cut(s) 147, 195, 198, 587, 1596, 2956
AclWI GGATC 7 cut(s) 952, 1183, 1326, 1604, 3097, 3331, 3344
AcsI RAATTY 4 cut(s) 731, 2344, 2884, 2894
AcuI CTGAAG 5 cut(s) 100, 268, 876, 1476, 2615
AcyI GRCGYC 1 cut(s) 2055
AdeI CACNNNGTG 1 cut(s) 2811
AfaI GTAC 5 cut(s) 1151, 1185, 1456, 2478, 3300
AfeI AGCGCT 1 cut(s) 2385
AfiI CCNNNNNNNGG 7 cut(s) 353, 797, 1390, 2457, 2729, 3185, 3274
AflIII ACRYGT 1 cut(s) 3127
AjnI CCWGG 6 cut(s) 409, 783, 1483, 1646, 2487, 3184
AjuI GAANNNNNNNTTGG 4 cut(s) 2154, 2186, 2358, 2390
AleI CACNNNNGTG 1 cut(s) 722
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
Alw21I GWGCWC 2 cut(s) 1445, 2807
Alw26I GTCTC 5 cut(s) 963, 1868, 3009, 3241, 3314
AlwI GGATC 7 cut(s) 952, 1183, 1326, 1604, 3097, 3331, 3344
AlwNI CAGNNNCTG 2 cut(s) 416, 818
Ama87I CYCGRG 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 216
Aor51HI AGCGCT 1 cut(s) 2385
AoxI GGCC 6 cut(s) 232, 330, 1447, 1982, 2485, 2694
ApoI RAATTY 4 cut(s) 731, 2344, 2884, 2894
Asp700I GAANNNNTTC 2 cut(s) 1537, 1860
AspLEI GCGC 1 cut(s) 2386
AspS9I GGNCC 3 cut(s) 331, 1566, 1799
AsuII TTCGAA 1 cut(s) 3166
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 2 cut(s) 1566, 1799
AxyI CCTNAGG 1 cut(s) 1271
BaeI ACNNNNGTAYC 2 cut(s) 1141, 1174
BamHI GGATCC 1 cut(s) 3336
BanII GRGCYC 2 cut(s) 70, 3252
BbsI GAAGAC 2 cut(s) 2392, 2754
Bbv12I GWGCWC 2 cut(s) 1445, 2807
BbvCI CCTCAGC 1 cut(s) 3263
BciT130I CCWGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
BciVI GTATCC 1 cut(s) 2454
BclI TGATCA 1 cut(s) 1069
BcoDI GTCTC 5 cut(s) 963, 1868, 3009, 3241, 3314
BfaI CTAG 8 cut(s) 36, 51, 104, 180, 1125, 1286, 2240, 2619
BfmI CTRYAG 3 cut(s) 298, 1881, 2670
BfoI RGCGCY 1 cut(s) 2387
BfuAI ACCTGC 3 cut(s) 2635, 2950, 2956
BfuI GTATCC 1 cut(s) 2454
BglII AGATCT 2 cut(s) 1495, 2194
BlpI GCTNAGC 1 cut(s) 924
BmcAI AGTACT 2 cut(s) 1456, 2478
Bme1390I CCNGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
Bme18I GGWCC 2 cut(s) 1566, 1799
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 3 cut(s) 331, 1566, 1799
BmiI GGNNCC 4 cut(s) 333, 789, 1568, 3338
BmrFI CCNGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
BmrI ACTGGG 1 cut(s) 2601
BmuI ACTGGG 1 cut(s) 2601
BpiI GAAGAC 2 cut(s) 2392, 2754
BplI GAGNNNNNCTC 4 cut(s) 222, 254, 946, 978
Bpu10I CCTNAGC 2 cut(s) 2456, 3263
Bpu1102I GCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 3166
BpuEI CTTGAG 4 cut(s) 154, 613, 2965, 3223
BsaHI GRCGYC 1 cut(s) 2055
BsaI GGTCTC 1 cut(s) 963
BsaJI CCNNGG 9 cut(s) 409, 489, 1389, 1646, 1697, 2389, 2902, 3000, 3120
BsaWI WCCGGW 2 cut(s) 216, 574
Bsc4I CCNNNNNNNGG 7 cut(s) 353, 797, 1390, 2457, 2729, 3185, 3274
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 6 cut(s) 554, 1801, 1915, 2596, 3067, 3360
Bse21I CCTNAGG 1 cut(s) 1271
Bse3DI GCAATG 5 cut(s) 190, 422, 1754, 2045, 2314
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
BseDI CCNNGG 9 cut(s) 409, 489, 1389, 1646, 1697, 2389, 2902, 3000, 3120
BseLI CCNNNNNNNGG 7 cut(s) 353, 797, 1390, 2457, 2729, 3185, 3274
BseMI GCAATG 5 cut(s) 190, 422, 1754, 2045, 2314
BseMII CTCAG 6 cut(s) 165, 756, 938, 1229, 3057, 3277
BseNI ACTGG 6 cut(s) 554, 1801, 1915, 2596, 3067, 3360
BseRI GAGGAG 3 cut(s) 951, 2879, 3327
BseYI CCCAGC 2 cut(s) 811, 3178
BsgI GTGCAG 3 cut(s) 1326, 2259, 2984
Bsh1236I CGCG 1 cut(s) 3234
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 6 cut(s) 234, 332, 1449, 1984, 2487, 2696
BsiEI CGRYCG 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 1445, 2807
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 3 cut(s) 217, 235, 575
BslFI GGGAC 4 cut(s) 173, 391, 1552, 3148
BslI CCNNNNNNNGG 7 cut(s) 353, 797, 1390, 2457, 2729, 3185, 3274
BsmAI GTCTC 5 cut(s) 963, 1868, 3009, 3241, 3314
BsmBI CGTCTC 1 cut(s) 3241
BsmFI GGGAC 4 cut(s) 173, 391, 1552, 3148
BsmI GAATGC 1 cut(s) 3195
BsnI GGCC 6 cut(s) 234, 332, 1449, 1984, 2487, 2696
Bso31I GGTCTC 1 cut(s) 963
BsoBI CYCGRG 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 3166
Bsp1286I GDGCHC 4 cut(s) 70, 1445, 2807, 3252
Bsp13I TCCGGA 1 cut(s) 216
Bsp1407I TGTACA 2 cut(s) 1183, 3298
Bsp1720I GCTNAGC 1 cut(s) 924
Bsp19I CCATGG 2 cut(s) 1697, 3000
BspACI CCGC 6 cut(s) 147, 195, 198, 587, 1596, 2956
BspANI GGCC 6 cut(s) 234, 332, 1449, 1984, 2487, 2696
BspCNI CTCAG 6 cut(s) 164, 757, 937, 1228, 3056, 3276
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 1 cut(s) 3234
BspHI TCATGA 5 cut(s) 736, 964, 2437, 3220, 3285
BspLI GGNNCC 4 cut(s) 333, 789, 1568, 3338
BspMAI CTGCAG 2 cut(s) 1885, 2674
BspMI ACCTGC 3 cut(s) 2635, 2950, 2956
BspPI GGATC 7 cut(s) 952, 1183, 1326, 1604, 3097, 3331, 3344
BspQI GCTCTTC 2 cut(s) 52, 2795
BspT104I TTCGAA 1 cut(s) 3166
BspTNI GGTCTC 1 cut(s) 963
BsrBI CCGCTC 2 cut(s) 1596, 2956
BsrDI GCAATG 5 cut(s) 190, 422, 1754, 2045, 2314
BsrFI RCCGGY 1 cut(s) 234
BsrGI TGTACA 2 cut(s) 1183, 3298
BsrI ACTGG 6 cut(s) 554, 1801, 1915, 2596, 3067, 3360
BssAI RCCGGY 1 cut(s) 234
BssECI CCNNGG 9 cut(s) 409, 489, 1389, 1646, 1697, 2389, 2902, 3000, 3120
BssNI GRCGYC 1 cut(s) 2055
BssT1I CCWWGG 4 cut(s) 1697, 2389, 3000, 3120
Bst2UI CCWGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
Bst4CI ACNGT 9 cut(s) 132, 161, 302, 343, 981, 1347, 2312, 2407, 3097
Bst6I CTCTTC 2 cut(s) 52, 2795
BstACI GRCGYC 1 cut(s) 2055
BstAPI GCANNNNNTGC 2 cut(s) 643, 2971
BstAUI TGTACA 2 cut(s) 1183, 3298
BstBI TTCGAA 1 cut(s) 3166
BstC8I GCNNGC 7 cut(s) 236, 395, 612, 1477, 1971, 2485, 2667
BstDSI CCRYGG 2 cut(s) 1697, 3000
BstEII GGTNACC 1 cut(s) 1378
BstFNI CGCG 1 cut(s) 3234
BstH2I RGCGCY 1 cut(s) 2387
BstHHI GCGC 1 cut(s) 2386
BstMAI GTCTC 5 cut(s) 963, 1868, 3009, 3241, 3314
BstMCI CGRYCG 1 cut(s) 134
BstNI CCWGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
BstNSI RCATGY 4 cut(s) 1158, 1267, 2035, 2142
BstPI GGTNACC 1 cut(s) 1378
BstSCI CCNGG 6 cut(s) 409, 783, 1483, 1646, 2487, 3184
BstSFI CTRYAG 3 cut(s) 298, 1881, 2670
BstUI CGCG 1 cut(s) 3234
BstV2I GAAGAC 2 cut(s) 2392, 2754
BstX2I RGATCY 5 cut(s) 1188, 1318, 1495, 2194, 3336
BstYI RGATCY 5 cut(s) 1188, 1318, 1495, 2194, 3336
Bsu36I CCTNAGG 1 cut(s) 1271
BsuI GTATCC 1 cut(s) 2454
BsuRI GGCC 6 cut(s) 234, 332, 1449, 1984, 2487, 2696
BtgI CCRYGG 2 cut(s) 1697, 3000
BtgZI GCGATG 1 cut(s) 1518
BtsI GCAGTG 3 cut(s) 579, 1572, 3301
BveI ACCTGC 3 cut(s) 2635, 2950, 2956
Cac8I GCNNGC 7 cut(s) 236, 395, 612, 1477, 1971, 2485, 2667
CaiI CAGNNNCTG 2 cut(s) 416, 818
CciI TCATGA 5 cut(s) 736, 964, 2437, 3220, 3285
CfoI GCGC 1 cut(s) 2386
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 3 cut(s) 331, 1566, 1799
CseI GACGC 2 cut(s) 2044, 3223
Csp6I GTAC 5 cut(s) 1150, 1184, 1455, 2477, 3299
CspCI CAANNNNNGTGG 4 cut(s) 2001, 2036, 2109, 2144
CviQI GTAC 5 cut(s) 1150, 1184, 1455, 2477, 3299
DraIII CACNNNGTG 1 cut(s) 2811
Eam1104I CTCTTC 2 cut(s) 52, 2795
EarI CTCTTC 2 cut(s) 52, 2795
EciI GGCGGA 2 cut(s) 136, 184
Eco130I CCWWGG 4 cut(s) 1697, 2389, 3000, 3120
Eco147I AGGCCT 1 cut(s) 1449
Eco24I GRGCYC 2 cut(s) 70, 3252
Eco31I GGTCTC 1 cut(s) 963
Eco47I GGWCC 2 cut(s) 1566, 1799
Eco47III AGCGCT 1 cut(s) 2385
Eco57I CTGAAG 5 cut(s) 100, 268, 876, 1476, 2615
Eco81I CCTNAGG 1 cut(s) 1271
Eco88I CYCGRG 1 cut(s) 490
Eco91I GGTNACC 1 cut(s) 1378
EcoO65I GGTNACC 1 cut(s) 1378
EcoRI GAATTC 2 cut(s) 2884, 2894
EcoRII CCWGG 6 cut(s) 409, 783, 1483, 1646, 2487, 3184
EcoT14I CCWWGG 4 cut(s) 1697, 2389, 3000, 3120
EcoT22I ATGCAT 2 cut(s) 2140, 2976
EcoT38I GRGCYC 2 cut(s) 70, 3252
ErhI CCWWGG 4 cut(s) 1697, 2389, 3000, 3120
Esp3I CGTCTC 1 cut(s) 3241
FalI AAGNNNNNCTT 6 cut(s) 31, 63, 441, 473, 2535, 2567
FaqI GGGAC 4 cut(s) 173, 391, 1552, 3148
FauI CCCGC 1 cut(s) 580
FbaI TGATCA 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 111
FriOI GRGCYC 2 cut(s) 70, 3252
FspBI CTAG 8 cut(s) 36, 51, 104, 180, 1125, 1286, 2240, 2619
GlaI GCGC 1 cut(s) 2385
GsaI CCCAGC 2 cut(s) 815, 3182
HaeII RGCGCY 1 cut(s) 2387
HaeIII GGCC 6 cut(s) 234, 332, 1449, 1984, 2487, 2696
HapII CCGG 3 cut(s) 217, 235, 575
HgaI GACGC 2 cut(s) 2044, 3223
HhaI GCGC 1 cut(s) 2386
Hin1I GRCGYC 1 cut(s) 2055
Hin6I GCGC 1 cut(s) 2384
HinP1I GCGC 1 cut(s) 2384
HincII GTYRAC 2 cut(s) 157, 2997
HindII GTYRAC 2 cut(s) 157, 2997
HindIII AAGCTT 3 cut(s) 366, 608, 2453
HpaII CCGG 3 cut(s) 217, 235, 575
Hpy166II GTNNAC 6 cut(s) 112, 157, 2997, 3072, 3299, 3361
Hpy188I TCNGA 7 cut(s) 119, 127, 1390, 1495, 2323, 2513, 3046
Hpy8I GTNNAC 6 cut(s) 112, 157, 2997, 3072, 3299, 3361
Hpy99I CGWCG 2 cut(s) 1689, 2060
HpyCH4III ACNGT 9 cut(s) 132, 161, 302, 343, 981, 1347, 2312, 2407, 3097
HpyCH4IV ACGT 2 cut(s) 2538, 3127
HpySE526I ACGT 2 cut(s) 2538, 3127
Hsp92I GRCGYC 1 cut(s) 2055
HspAI GCGC 1 cut(s) 2384
Kpn2I TCCGGA 1 cut(s) 216
KroI GCCGGC 1 cut(s) 234
KroNI GCCGGC 1 cut(s) 236
Ksp22I TGATCA 1 cut(s) 1069
LguI GCTCTTC 2 cut(s) 52, 2795
LmnI GCTCC 7 cut(s) 1063, 1272, 1337, 1593, 2391, 2953, 3314
MaeI CTAG 8 cut(s) 36, 51, 104, 180, 1125, 1286, 2240, 2619
MaeII ACGT 2 cut(s) 2538, 3127
MbiI CCGCTC 2 cut(s) 1596, 2956
MfeI CAATTG 2 cut(s) 2013, 2822
MflI RGATCY 5 cut(s) 1188, 1318, 1495, 2194, 3336
MhlI GDGCHC 4 cut(s) 70, 1445, 2807, 3252
MlyI GAGTC 3 cut(s) 79, 943, 2956
MmeI TCCRAC 4 cut(s) 303, 1381, 2233, 3140
Mph1103I ATGCAT 2 cut(s) 2140, 2976
MroI TCCGGA 1 cut(s) 216
MroNI GCCGGC 1 cut(s) 234
MroXI GAANNNNTTC 2 cut(s) 1537, 1860
MslI CAYNNNNRTG 5 cut(s) 473, 667, 722, 741, 2879
MspA1I CMGCKG 6 cut(s) 200, 1738, 1880, 1943, 2675, 3375
MspI CCGG 3 cut(s) 217, 235, 575
MspR9I CCNGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
MunI CAATTG 2 cut(s) 2013, 2822
Mva1269I GAATGC 1 cut(s) 3195
MvaI CCWGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
MvnI CGCG 1 cut(s) 3234
NaeI GCCGGC 1 cut(s) 236
NcoI CCATGG 2 cut(s) 1697, 3000
NgoMIV GCCGGC 1 cut(s) 234
NlaIV GGNNCC 4 cut(s) 333, 789, 1568, 3338
NmeAIII GCCGAG 3 cut(s) 672, 2174, 3231
NmuCI GTSAC 7 cut(s) 577, 1024, 1037, 1046, 1259, 1378, 1792
NsiI ATGCAT 2 cut(s) 2140, 2976
NspI RCATGY 4 cut(s) 1158, 1267, 2035, 2142
NspV TTCGAA 1 cut(s) 3166
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 5 cut(s) 736, 964, 2437, 3220, 3285
PaqCI CACCTGC 2 cut(s) 2635, 2950
PceI AGGCCT 1 cut(s) 1449
PciSI GCTCTTC 2 cut(s) 52, 2795
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 1 cut(s) 3195
PdiI GCCGGC 1 cut(s) 236
PdmI GAANNNNTTC 2 cut(s) 1537, 1860
PflMI CCANNNNNTGG 2 cut(s) 353, 3274
PleI GAGTC 3 cut(s) 79, 942, 2955
PpsI GAGTC 3 cut(s) 79, 942, 2955
PsiI TTATAA 1 cut(s) 606
Psp6I CCWGG 6 cut(s) 409, 783, 1483, 1646, 2487, 3184
PspEI GGTNACC 1 cut(s) 1378
PspFI CCCAGC 2 cut(s) 811, 3178
PspGI CCWGG 6 cut(s) 409, 783, 1483, 1646, 2487, 3184
PspN4I GGNNCC 4 cut(s) 333, 789, 1568, 3338
PspPI GGNCC 3 cut(s) 331, 1566, 1799
PstI CTGCAG 2 cut(s) 1885, 2674
PstNI CAGNNNCTG 2 cut(s) 416, 818
PsuI RGATCY 5 cut(s) 1188, 1318, 1495, 2194, 3336
PvuII CAGCTG 5 cut(s) 1738, 1880, 1943, 2675, 3375
RsaI GTAC 5 cut(s) 1151, 1185, 1456, 2478, 3300
RsaNI GTAC 5 cut(s) 1150, 1184, 1455, 2477, 3299
RseI CAYNNNNRTG 5 cut(s) 473, 667, 722, 741, 2879
SapI GCTCTTC 2 cut(s) 52, 2795
Sau96I GGNCC 3 cut(s) 331, 1566, 1799
ScaI AGTACT 2 cut(s) 1456, 2478
SchI GAGTC 3 cut(s) 79, 943, 2956
ScrFI CCNGG 6 cut(s) 411, 785, 1485, 1648, 2489, 3186
SduI GDGCHC 4 cut(s) 70, 1445, 2807, 3252
SfcI CTRYAG 3 cut(s) 298, 1881, 2670
SfuI TTCGAA 1 cut(s) 3166
SinI GGWCC 2 cut(s) 1566, 1799
SmiMI CAYNNNNRTG 5 cut(s) 473, 667, 722, 741, 2879
SmlI CTYRAG 4 cut(s) 169, 628, 2944, 3238
SmoI CTYRAG 4 cut(s) 169, 628, 2944, 3238
SseBI AGGCCT 1 cut(s) 1449
SsiI CCGC 6 cut(s) 147, 195, 198, 587, 1596, 2956
SspI AATATT 3 cut(s) 352, 1768, 2292
SspMI CTAG 8 cut(s) 36, 51, 104, 180, 1125, 1286, 2240, 2619
StuI AGGCCT 1 cut(s) 1449
StyD4I CCNGG 6 cut(s) 409, 783, 1483, 1646, 2487, 3184
StyI CCWWGG 4 cut(s) 1697, 2389, 3000, 3120
TaaI ACNGT 9 cut(s) 132, 161, 302, 343, 981, 1347, 2312, 2407, 3097
TaiI ACGT 2 cut(s) 2541, 3130
TaqI TCGA 5 cut(s) 520, 2058, 2380, 3159, 3166
TaqII GACCGA 2 cut(s) 1554, 2315
TatI WGTACW 4 cut(s) 1183, 1454, 2476, 3298
TauI GCSGC 2 cut(s) 200, 2959
TseFI GTSAC 7 cut(s) 577, 1024, 1037, 1046, 1259, 1378, 1792
Tsp45I GTSAC 7 cut(s) 577, 1024, 1037, 1046, 1259, 1378, 1792
TspGWI ACGGA 1 cut(s) 1125
Van91I CCANNNNNTGG 2 cut(s) 353, 3274
VpaK11BI GGWCC 2 cut(s) 1566, 1799
XapI RAATTY 4 cut(s) 731, 2344, 2884, 2894
XbaI TCTAGA 2 cut(s) 35, 50
XceI RCATGY 4 cut(s) 1158, 1267, 2035, 2142
XcmI CCANNNNNNNNNTGG 1 cut(s) 1855
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 2 cut(s) 1537, 1860
XspI CTAG 8 cut(s) 36, 51, 104, 180, 1125, 1286, 2240, 2619
ZrmI AGTACT 2 cut(s) 1456, 2478
Zsp2I ATGCAT 2 cut(s) 2140, 2976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.