Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003814

Physical Location & Seq

Genomic Coordinates Forward (+)

14435068 .. 14440341

Gene Structure 5274 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1171100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGAAGCAGGCGGAGCTCACGGCGCTGCTCTGCCTGATATGGGCGGCGACTCTCCTCTACGGCGAGATGTTCGCCTACTGGAGGCCCTTTCTCTCGTCCTGCTCGTGGCCGCACCTCAATCGCACTTCTTCCTCCACAGCCGACCCCGATTATGTCAAGGTGGCGGTCATCACCGACCCGCAGCTCATGGACCGGACCTCCCTCCCTCTTCCGCCCAAATCACTCGCTCTCGAACTGGCGCAGTTCTTCACCGACCTGTACATGCGCAGAGCCTTTCATCAATCTGTACTTCCTCTCCGGCCCGATGTTATTTTCTTCTTAGGTGACTATTTCGATGGCGGTCCTTATCTTTTGGATGAAGAGTGGAGCGAATCATTGAGCCGTCTCAGACATATTTTTTCACTGAGGTTGCCAGATAGATATTTAAATATCCCAGTTTACTATCTTCCTGGAAACCATGACATTGGCTATGGAAGTCTCCACTCTCGTAAGGCACAGGCAATTAGACGATATGAAAAGGAATTTGGTAGCAGAAACTATCGATTTACAGTTGGAAAAGTGGAGTTCATTGCCATTGATGCTCAAACTATTGATGGAAATCCTCAAGGAGTTCTTGCTTCCTCTACACGTGATTTTATCAAGAATGTCTCCATGGATGTTCAAGTGTCTCCAAGGGTTTTATTGACCCATATTCCATTGTATCGACGGGATTGGACAGATTGTGGCCCCAACCGTAACTCAGAAATTATCAACCAGCGGATTCTGCGATCTGCTGACGGTCAAGAAGTATTGTATCAGAATTACATTACAGAGGAATCATCAAACTACTTATTGGATTCAATCAAACCGGTACTTGTTTTATCCGGTCATGATCATGATCAGTGCCATGTGATCCATAAGTCGAAAAAGGGGCCTGTAGGGGAGTACACTGTAGGGACAGTGAGTTGGCAGCAGGGAAACTTGTATCCATCTTTCATGTTATTGTCTACCAGTAATTTTGTACCTTCAAATGCATCCAGTTCTGAAGAGGCAATCTTAACTCATTTGTGCTTTCTTCCTATGCAAACACACATTTATTTATGGTATCTCTCACTATTTGTTTTTACCCTTGTTTCCCTCCTGCTTTGGCCATCTGGTGGTGTGAGTGTTTGGCATCATTGTAGTGATTTTATGGGGATTGTAAAACAAGTAATCAGTAGCATCAGAAGTAGAACAAAAGAGAAAATTGAAGATCTAAACTGTGAATATGAGATGATTTGGGATGCAGAAGGATCAATGCATCTTGTCAAGAAAGCCTTGGGCACTCCTATTGCATGTTCAAGCGAGAGAAACTCAGTTGAAAGAGGTAATGCTGTGATGCGGCCCACAGCTAGAAAGAATACTTCCCAGGACATAGAGGCTTCTGTGAATGGAGACACTGAAGCCGATTCTGTGGTTGATCCTATGGCAAAATTACTGCCTAGAGCAAGCAGATCATGGACAAAGACGATGATCAGAAGATTGGTACGGACACTTCGAATGGTCATGATTATCGCTGCAGTAAATATACCCCTCTATGTGATGTTACTTTTCAAGGATTGGATTGACCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016192 GO:0019538 GO:0019637 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0071704 GO:0090304 GO:0090407 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos

Protein Analysis

531

Amino Acids

60.45

Weight (kDa)

6.95

Isoelectric Point (pI)

44.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Metallophos	PF00149	53 - 293	5.9e-13	Calcineurin-like phosphoesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53710 AT1G53710
fragaria_vesca	FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250 FvH4_6g36250
malus_domestica	MD09G1171100.v1.1 MD17G1156100.v1.1
prunus_persica	Prupe.3G029600_v2.0.a1
pyrus_communis	pycom09g08860 pycom17g14900 pycom17g14910
rosa_chinensis	RchiOBHm_Chr2g0148251
rosa_laevigata	RLG00000020329
rosa_multiflora	Rmu_co8220218.1_g000001 Rmu_sc0001983.1_g000004 Rmu_sc0003937.1_g000004 Rmu_sc0029680.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098970
rosa_rugosa	Rorug02G0405000 Rorug02G0405000 Rorug05G0121000
rosa_samantha	Rh2AG463600 Rh2BG476700 Rh2CG450400 Rh2DG485400 Rh5AG221700
rosa_wichuraiana	Rw2G037790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	266
AccB7I	CCANNNNNTGG	1 cut(s)	1136
AccI	GTMKAC	1 cut(s)	986
AciI	CCGC	9 cut(s)	11, 44, 110, 164, 179, 212, 339, 757, 1360
AclWI	GGATC	3 cut(s)	886, 1279, 1433
AcoI	YGGCCR	2 cut(s)	107, 1127
AcsI	RAATTY	1 cut(s)	521
AcuI	CTGAAG	2 cut(s)	1044, 1440
AcvI	CACGTG	1 cut(s)	629
AfaI	GTAC	6 cut(s)	260, 288, 852, 926, 1002, 1506
AfiI	CCNNNNNNNGG	4 cut(s)	40, 81, 105, 1136
AflIII	ACRYGT	1 cut(s)	626
AgeI	ACCGGT	1 cut(s)	847
AgsI	TTSAA	7 cut(s)	662, 840, 1008, 1229, 1320, 1340, 1573
AjnI	CCWGG	2 cut(s)	448, 1386
AjuI	GAANNNNNNNTTGG	2 cut(s)	507, 539
AluBI	AGCT	3 cut(s)	16, 184, 1370
AluI	AGCT	3 cut(s)	16, 184, 1370
Alw21I	GWGCWC	1 cut(s)	18
Alw26I	GTCTC	5 cut(s)	389, 482, 652, 672, 1407
AlwI	GGATC	3 cut(s)	886, 1279, 1433
AoxI	GGCC	7 cut(s)	83, 107, 299, 724, 911, 1127, 1361
ApeKI	GCWGC	4 cut(s)	25, 181, 949, 1535
ApoI	RAATTY	1 cut(s)	521
AsiGI	ACCGGT	1 cut(s)	847
AspLEI	GCGC	3 cut(s)	25, 241, 267
AspS9I	GGNCC	8 cut(s)	84, 190, 195, 300, 341, 725, 911, 1362
AsuHPI	GGTGA	3 cut(s)	163, 241, 335
AsuII	TTCGAA	1 cut(s)	1516
AvaII	GGWCC	3 cut(s)	190, 195, 341
BaeGI	GKGCMC	1 cut(s)	1304
BalI	TGGCCA	1 cut(s)	1129
BanII	GRGCYC	1 cut(s)	18
BarI	GAAGNNNNNNTAC	2 cut(s)	1497, 1529
BauI	CACGAG	1 cut(s)	103
BbrPI	CACGTG	1 cut(s)	629
Bbv12I	GWGCWC	1 cut(s)	18
BbvI	GCAGC	4 cut(s)	12, 193, 961, 1522
BccI	CCATC	4 cut(s)	329, 587, 976, 1138
BceAI	ACGGC	3 cut(s)	36, 76, 366
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	2 cut(s)	450, 1388
BciVI	GTATCC	1 cut(s)	975
BclI	TGATCA	3 cut(s)	871, 877, 1491
BcoDI	GTCTC	5 cut(s)	389, 482, 652, 672, 1407
BfaI	CTAG	2 cut(s)	1371, 1461
BfmI	CTRYAG	3 cut(s)	915, 930, 1536
BfoI	RGCGCY	1 cut(s)	26
BfuI	GTATCC	1 cut(s)	975
BglII	AGATCT	1 cut(s)	1231
BisI	GCNGC	7 cut(s)	26, 45, 110, 182, 950, 1361, 1536
BlsI	GCNGC	7 cut(s)	27, 46, 111, 183, 951, 1362, 1537
Bme1390I	CCNGG	2 cut(s)	450, 1388
Bme18I	GGWCC	3 cut(s)	190, 195, 341
BmgT120I	GGNCC	8 cut(s)	84, 190, 195, 300, 341, 725, 911, 1362
BmiI	GGNNCC	2 cut(s)	727, 912
BmrFI	CCNGG	2 cut(s)	450, 1388
BmrI	ACTGGG	1 cut(s)	428
BmsI	GCATC	7 cut(s)	568, 1022, 1162, 1209, 1252, 1288, 1347
BmuI	ACTGGG	1 cut(s)	428
BplI	GAGNNNNNCTC	2 cut(s)	1316, 1348
BpmI	CTGGAG	1 cut(s)	100
Bpu14I	TTCGAA	1 cut(s)	1516
BpuEI	CTTGAG	1 cut(s)	588
Bsa29I	ATCGAT	1 cut(s)	541
BsaAI	YACGTR	1 cut(s)	629
BsaBI	GATNNNNATC	2 cut(s)	597, 876
BsaJI	CCNNGG	4 cut(s)	651, 671, 1296, 1386
BsaWI	WCCGGW	3 cut(s)	192, 847, 863
BsaXI	ACNNNNNCTCC	4 cut(s)	182, 212, 279, 309
Bsc4I	CCNNNNNNNGG	4 cut(s)	40, 81, 105, 1136
Bse118I	RCCGGY	1 cut(s)	847
Bse1I	ACTGG	5 cut(s)	83, 240, 434, 990, 1017
Bse3DI	GCAATG	1 cut(s)	567
Bse8I	GATNNNNATC	2 cut(s)	597, 876
BseBI	CCWGG	2 cut(s)	450, 1388
BseCI	ATCGAT	1 cut(s)	541
BseDI	CCNNGG	4 cut(s)	651, 671, 1296, 1386
BseGI	GGATG	4 cut(s)	361, 661, 1013, 1267
BseJI	GATNNNNATC	2 cut(s)	597, 876
BseLI	CCNNNNNNNGG	4 cut(s)	40, 81, 105, 1136
BseMI	GCAATG	1 cut(s)	567
BseMII	CTCAG	4 cut(s)	395, 400, 753, 1347
BseNI	ACTGG	5 cut(s)	83, 240, 434, 990, 1017
BseRI	GAGGAG	1 cut(s)	44
BseSI	GKGCMC	1 cut(s)	1304
BseXI	GCAGC	4 cut(s)	12, 193, 961, 1522
BshFI	GGCC	7 cut(s)	85, 109, 301, 726, 913, 1129, 1363
BshTI	ACCGGT	1 cut(s)	847
BshVI	ATCGAT	1 cut(s)	541
BsiHKAI	GWGCWC	1 cut(s)	18
BsiSI	CCGG	4 cut(s)	193, 298, 848, 864
BslFI	GGGAC	1 cut(s)	949
BslI	CCNNNNNNNGG	4 cut(s)	40, 81, 105, 1136
BsmAI	GTCTC	5 cut(s)	389, 482, 652, 672, 1407
BsmBI	CGTCTC	1 cut(s)	389
BsmFI	GGGAC	1 cut(s)	949
BsnI	GGCC	7 cut(s)	85, 109, 301, 726, 913, 1129, 1363
Bsp119I	TTCGAA	1 cut(s)	1516
Bsp1286I	GDGCHC	2 cut(s)	18, 1304
Bsp1407I	TGTACA	1 cut(s)	258
Bsp143I	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
Bsp19I	CCATGG	1 cut(s)	651
BspACI	CCGC	9 cut(s)	11, 44, 110, 164, 179, 212, 339, 757, 1360
BspANI	GGCC	7 cut(s)	85, 109, 301, 726, 913, 1129, 1363
BspCNI	CTCAG	4 cut(s)	396, 399, 752, 1346
BspDI	ATCGAT	1 cut(s)	541
BspHI	TCATGA	3 cut(s)	868, 874, 1524
BspLI	GGNNCC	2 cut(s)	727, 912
BspMAI	CTGCAG	1 cut(s)	1540
BspPI	GGATC	3 cut(s)	886, 1279, 1433
BspT104I	TTCGAA	1 cut(s)	1516
BsrDI	GCAATG	1 cut(s)	567
BsrFI	RCCGGY	1 cut(s)	847
BsrGI	TGTACA	1 cut(s)	258
BsrI	ACTGG	5 cut(s)	83, 240, 434, 990, 1017
BssAI	RCCGGY	1 cut(s)	847
BssECI	CCNNGG	4 cut(s)	651, 671, 1296, 1386
BssMI	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
BssSI	CACGAG	1 cut(s)	103
BssT1I	CCWWGG	3 cut(s)	651, 671, 1296
Bst2BI	CACGAG	1 cut(s)	103
Bst2UI	CCWGG	2 cut(s)	450, 1388
Bst4CI	ACNGT	6 cut(s)	550, 734, 779, 931, 940, 1241
Bst6I	CTCTTC	3 cut(s)	213, 354, 1020
BstAUI	TGTACA	1 cut(s)	258
BstBAI	YACGTR	1 cut(s)	629
BstBI	TTCGAA	1 cut(s)	1516
BstC8I	GCNNGC	2 cut(s)	9, 1468
BstDEI	CTNAG	5 cut(s)	319, 386, 404, 739, 1333
BstDSI	CCRYGG	1 cut(s)	651
BstENI	CCTNNNNNAGG	1 cut(s)	79
BstF5I	GGATG	4 cut(s)	361, 661, 1013, 1267
BstH2I	RGCGCY	1 cut(s)	26
BstHHI	GCGC	3 cut(s)	25, 241, 267
BstKTI	GATC	9 cut(s)	770, 874, 880, 894, 1234, 1274, 1441, 1475, 1494
BstMAI	GTCTC	5 cut(s)	389, 482, 652, 672, 1407
BstMBI	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
BstMWI	GCNNNNNNNGC	4 cut(s)	13, 22, 578, 763
BstNI	CCWGG	2 cut(s)	450, 1388
BstNSI	RCATGY	2 cut(s)	265, 1317
BstSCI	CCNGG	2 cut(s)	448, 1386
BstSFI	CTRYAG	3 cut(s)	915, 930, 1536
BstSLI	GKGCMC	1 cut(s)	1304
BstV1I	GCAGC	4 cut(s)	12, 193, 961, 1522
BstX2I	RGATCY	1 cut(s)	1231
BstXI	CCANNNNNNTGG	1 cut(s)	464
BstYI	RGATCY	1 cut(s)	1231
Bsu15I	ATCGAT	1 cut(s)	541
BsuI	GTATCC	1 cut(s)	975
BsuRI	GGCC	7 cut(s)	85, 109, 301, 726, 913, 1129, 1363
BsuTUI	ATCGAT	1 cut(s)	541
BtgI	CCRYGG	1 cut(s)	651
BtsCI	GGATG	4 cut(s)	361, 661, 1013, 1267
BtsIMutI	CAGTG	5 cut(s)	401, 887, 927, 945, 1416
Cac8I	GCNNGC	2 cut(s)	9, 1468
CciI	TCATGA	3 cut(s)	868, 874, 1524
CfoI	GCGC	3 cut(s)	25, 241, 267
Cfr10I	RCCGGY	1 cut(s)	847
Cfr13I	GGNCC	8 cut(s)	84, 190, 195, 300, 341, 725, 911, 1362
ClaI	ATCGAT	1 cut(s)	541
Csp6I	GTAC	6 cut(s)	259, 287, 851, 925, 1001, 1505
CspAI	ACCGGT	1 cut(s)	847
CviQI	GTAC	6 cut(s)	259, 287, 851, 925, 1001, 1505
DdeI	CTNAG	5 cut(s)	319, 386, 404, 739, 1333
DpnI	GATC	9 cut(s)	769, 873, 879, 893, 1233, 1273, 1440, 1474, 1493
DpnII	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
DraI	TTTAAA	1 cut(s)	426
EaeI	YGGCCR	2 cut(s)	107, 1127
Eam1104I	CTCTTC	3 cut(s)	213, 354, 1020
EarI	CTCTTC	3 cut(s)	213, 354, 1020
EciI	GGCGGA	2 cut(s)	26, 201
Ecl136II	GAGCTC	1 cut(s)	16
Eco130I	CCWWGG	3 cut(s)	651, 671, 1296
Eco24I	GRGCYC	1 cut(s)	18
Eco47I	GGWCC	3 cut(s)	190, 195, 341
Eco53kI	GAGCTC	1 cut(s)	16
Eco57I	CTGAAG	2 cut(s)	1044, 1440
Eco72I	CACGTG	1 cut(s)	629
EcoICRI	GAGCTC	1 cut(s)	16
EcoNI	CCTNNNNNAGG	1 cut(s)	79
EcoO109I	RGGNCCY	2 cut(s)	84, 911
EcoRII	CCWGG	2 cut(s)	448, 1386
EcoT14I	CCWWGG	3 cut(s)	651, 671, 1296
EcoT22I	ATGCAT	2 cut(s)	1015, 1281
EcoT38I	GRGCYC	1 cut(s)	18
ErhI	CCWWGG	3 cut(s)	651, 671, 1296
Esp3I	CGTCTC	1 cut(s)	389
FalI	AAGNNNNNCTT	4 cut(s)	597, 629, 1280, 1312
FaqI	GGGAC	1 cut(s)	949
FauI	CCCGC	1 cut(s)	186
FbaI	TGATCA	3 cut(s)	871, 877, 1491
FblI	GTMKAC	1 cut(s)	986
Fnu4HI	GCNGC	7 cut(s)	26, 45, 110, 182, 950, 1361, 1536
FokI	GGATG	4 cut(s)	368, 668, 1000, 1274
FriOI	GRGCYC	1 cut(s)	18
Fsp4HI	GCNGC	7 cut(s)	26, 45, 110, 182, 950, 1361, 1536
FspBI	CTAG	2 cut(s)	1371, 1461
FspI	TGCGCA	1 cut(s)	266
GlaI	GCGC	3 cut(s)	24, 240, 266
GluI	GCNGC	7 cut(s)	26, 45, 110, 182, 950, 1361, 1536
GsuI	CTGGAG	1 cut(s)	100
HaeII	RGCGCY	1 cut(s)	26
HaeIII	GGCC	7 cut(s)	85, 109, 301, 726, 913, 1129, 1363
HapII	CCGG	4 cut(s)	193, 298, 848, 864
HhaI	GCGC	3 cut(s)	25, 241, 267
Hin6I	GCGC	3 cut(s)	23, 239, 265
HinP1I	GCGC	3 cut(s)	23, 239, 265
HinfI	GANTC	6 cut(s)	49, 371, 760, 815, 836, 1427
HpaII	CCGG	4 cut(s)	193, 298, 848, 864
HphI	GGTGA	3 cut(s)	163, 241, 335
Hpy166II	GTNNAC	3 cut(s)	439, 927, 987
Hpy188I	TCNGA	6 cut(s)	389, 742, 798, 1024, 1205, 1496
Hpy188III	TCNNGA	7 cut(s)	230, 640, 782, 869, 875, 1288, 1525
Hpy8I	GTNNAC	3 cut(s)	439, 927, 987
Hpy99I	CGWCG	1 cut(s)	708
HpyAV	CCTTC	2 cut(s)	1014, 1262
HpyCH4III	ACNGT	6 cut(s)	550, 734, 779, 931, 940, 1241
HpyCH4IV	ACGT	1 cut(s)	628
HpyCH4V	TGCA	6 cut(s)	1013, 1063, 1265, 1279, 1313, 1538
HpyF10VI	GCNNNNNNNGC	4 cut(s)	13, 22, 578, 763
HpyF3I	CTNAG	5 cut(s)	319, 386, 404, 739, 1333
HpySE526I	ACGT	1 cut(s)	628
HspAI	GCGC	3 cut(s)	23, 239, 265
Ksp22I	TGATCA	3 cut(s)	871, 877, 1491
Kzo9I	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
LmnI	GCTCC	2 cut(s)	13, 366
Lsp1109I	GCAGC	4 cut(s)	12, 193, 961, 1522
LweI	GCATC	7 cut(s)	568, 1022, 1162, 1209, 1252, 1288, 1347
MaeI	CTAG	2 cut(s)	1371, 1461
MaeII	ACGT	1 cut(s)	628
MaeIII	GTNAC	3 cut(s)	323, 734, 1563
MalI	GATC	9 cut(s)	769, 873, 879, 893, 1233, 1273, 1440, 1474, 1493
MboI	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
MflI	RGATCY	1 cut(s)	1231
MhlI	GDGCHC	2 cut(s)	18, 1304
MlsI	TGGCCA	1 cut(s)	1129
MluCI	AATT	7 cut(s)	501, 521, 744, 799, 994, 1224, 1451
MluNI	TGGCCA	1 cut(s)	1129
MlyI	GAGTC	1 cut(s)	43
MmeI	TCCRAC	1 cut(s)	532
Mox20I	TGGCCA	1 cut(s)	1129
Mph1103I	ATGCAT	2 cut(s)	1015, 1281
MscI	TGGCCA	1 cut(s)	1129
MseI	TTAA	2 cut(s)	425, 1037
MslI	CAYNNNNRTG	2 cut(s)	873, 1161
Msp20I	TGGCCA	1 cut(s)	1129
MspA1I	CMGCKG	1 cut(s)	757
MspI	CCGG	4 cut(s)	193, 298, 848, 864
MspR9I	CCNGG	2 cut(s)	450, 1388
MvaI	CCWGG	2 cut(s)	450, 1388
MwoI	GCNNNNNNNGC	4 cut(s)	13, 22, 578, 763
NcoI	CCATGG	1 cut(s)	651
NdeII	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
NlaIV	GGNNCC	2 cut(s)	727, 912
NmuCI	GTSAC	1 cut(s)	323
NsbI	TGCGCA	1 cut(s)	266
NsiI	ATGCAT	2 cut(s)	1015, 1281
NspI	RCATGY	2 cut(s)	265, 1317
NspV	TTCGAA	1 cut(s)	1516
PagI	TCATGA	3 cut(s)	868, 874, 1524
PcsI	WCGNNNNNNNCGW	2 cut(s)	101, 1513
PfeI	GAWTC	5 cut(s)	371, 760, 815, 836, 1427
PflMI	CCANNNNNTGG	1 cut(s)	1136
PfoI	TCCNGGA	1 cut(s)	448
PinAI	ACCGGT	1 cut(s)	847
PkrI	GCNGC	7 cut(s)	27, 46, 111, 183, 951, 1362, 1537
PleI	GAGTC	1 cut(s)	43
PmaCI	CACGTG	1 cut(s)	629
PmlI	CACGTG	1 cut(s)	629
PpsI	GAGTC	1 cut(s)	43
Ppu21I	YACGTR	1 cut(s)	629
Psp124BI	GAGCTC	1 cut(s)	18
Psp6I	CCWGG	2 cut(s)	448, 1386
PspCI	CACGTG	1 cut(s)	629
PspGI	CCWGG	2 cut(s)	448, 1386
PspN4I	GGNNCC	2 cut(s)	727, 912
PspPI	GGNCC	8 cut(s)	84, 190, 195, 300, 341, 725, 911, 1362
PstI	CTGCAG	1 cut(s)	1540
PsuI	RGATCY	1 cut(s)	1231
RsaI	GTAC	6 cut(s)	260, 288, 852, 926, 1002, 1506
RsaNI	GTAC	6 cut(s)	259, 287, 851, 925, 1001, 1505
RseI	CAYNNNNRTG	2 cut(s)	873, 1161
SacI	GAGCTC	1 cut(s)	18
SaqAI	TTAA	2 cut(s)	425, 1037
SatI	GCNGC	7 cut(s)	26, 45, 110, 182, 950, 1361, 1536
Sau3AI	GATC	9 cut(s)	767, 871, 877, 891, 1231, 1271, 1438, 1472, 1491
Sau96I	GGNCC	8 cut(s)	84, 190, 195, 300, 341, 725, 911, 1362
SchI	GAGTC	1 cut(s)	43
ScrFI	CCNGG	2 cut(s)	450, 1388
SduI	GDGCHC	2 cut(s)	18, 1304
SfaNI	GCATC	7 cut(s)	568, 1022, 1162, 1209, 1252, 1288, 1347
SfcI	CTRYAG	3 cut(s)	915, 930, 1536
SfuI	TTCGAA	1 cut(s)	1516
SinI	GGWCC	3 cut(s)	190, 195, 341
SmiI	ATTTAAAT	1 cut(s)	426
SmiMI	CAYNNNNRTG	2 cut(s)	873, 1161
SmlI	CTYRAG	1 cut(s)	603
SmoI	CTYRAG	1 cut(s)	603
Sse9I	AATT	7 cut(s)	501, 521, 744, 799, 994, 1224, 1451
SsiI	CCGC	9 cut(s)	11, 44, 110, 164, 179, 212, 339, 757, 1360
SspMI	CTAG	2 cut(s)	1371, 1461
SstI	GAGCTC	1 cut(s)	18
StyD4I	CCNGG	2 cut(s)	448, 1386
StyI	CCWWGG	3 cut(s)	651, 671, 1296
SwaI	ATTTAAAT	1 cut(s)	426
TaaI	ACNGT	6 cut(s)	550, 734, 779, 931, 940, 1241
TaiI	ACGT	1 cut(s)	631
TaqI	TCGA	6 cut(s)	231, 333, 541, 703, 902, 1516
TasI	AATT	7 cut(s)	501, 521, 744, 799, 994, 1224, 1451
TatI	WGTACW	3 cut(s)	258, 286, 924
TauI	GCSGC	3 cut(s)	47, 112, 1363
TfiI	GAWTC	5 cut(s)	371, 760, 815, 836, 1427
Tru1I	TTAA	2 cut(s)	425, 1037
Tru9I	TTAA	2 cut(s)	425, 1037
TscAI	CASTG	5 cut(s)	408, 887, 934, 945, 1423
TseFI	GTSAC	1 cut(s)	323
TseI	GCWGC	4 cut(s)	25, 181, 949, 1535
Tsp45I	GTSAC	1 cut(s)	323
TspDTI	ATGAA	6 cut(s)	17, 266, 372, 528, 556, 964
TspGWI	ACGGA	1 cut(s)	1522
TspRI	CASTG	5 cut(s)	408, 887, 934, 945, 1423
Van91I	CCANNNNNTGG	1 cut(s)	1136
VpaK11BI	GGWCC	3 cut(s)	190, 195, 341
XagI	CCTNNNNNAGG	1 cut(s)	79
XapI	RAATTY	1 cut(s)	521
XceI	RCATGY	2 cut(s)	265, 1317
XmiI	GTMKAC	1 cut(s)	986
XspI	CTAG	2 cut(s)	1371, 1461
Zsp2I	ATGCAT	2 cut(s)	1015, 1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.