Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002937

Physical Location & Seq

Genomic Coordinates Forward (+)

20892817 .. 20897749

Gene Structure 4933 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1213000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGAGTGTGGTGGGGTTTGATTTTGGCAATGAGAGCGGCATTGTGGCCGTAGCCAGGCAGAGGGGTATTGACGTTGTGCTCAATGATGAGTCCAAACGTGAAACTCCGGCCCTTGTTTGTTTCGGTGACAAGCAGCGGTTCATTGGGACAGCTGGGGCTGCTTCCGCATTGATGAACCCCAAGAACACAATTTCCCAGATTAAACGCTTGATTGGTAAGCAATTCTCTGATCCTGTGTTGCAGAGGGATATCAAGTCCTTGCCTTTTGCTGTTTCTGAAGGGCCTGATGGATATCCATTGATTCATGCCCGTTATTTGGGAGAAGCAAAGACATTTACACCTACCCAAGTTCTTGGAATGCTGTTTTCTGATCTGAAAATCATAGCCGAGAAGAATCTCAATGCAGCTGTTGTCGATTGCTGTATTGGGATTCCAGTGTATTTTACTGATCTCCAACGAAGAGCTGTTATGGATGCAGCAAAAATTGCTGGATTGAACCCTCTTCGCTTGTTCCATGAAACTACAGCAACTGCTTTGGCTTATGGTATTTACAAGACGGATTTACCAGAAAATGACCAATTGAATGTTGCCTTTGTTGATGTTGGGCATGCTAGCATGCAAGTTTGCATTGCTGGTTTCAAGAAAGGGCAGCTGAAAATATTGGCTCATTCCTTTGACCAGTCTTTGGGAGGTAGGGACTTTGATGAAGTACTGTTCCACCACTTTGCAGCAAAATTCAAGGAAGAGTACAAAATTGATGTTTTACAGAATGCAAGGGCTTGCCTTCGCCTTCGTACCGCTTGTGAGAAGTTGAAGAAGATGCTTAGTGCAAATCCTGTGGTACCTTTGAATATAGAGTGTTTAATGGAAGAGAAGGATGTTAGGGGAATTATTAAGCGGGATGAATTTGAGCAGATTAGTATTCCTATTTTGGAACGTGTGAAGGGACCTTTGGAGAAGGCCCTTCTTGATGCACAGCTTTCAATAGAGGACATTCACACAGTTGAGGTTGTCGGTTCAGGCTCTCGTGTTCCTGCTATAATCAAGATATTGACAGATTTCTTCAAAAAGGAGCCTAGGCGAACTATGAATGCAAGTGAGTGCGTTGCCAGAGGTTGTGCTTTGCAATGTGCAATTCTTAGTCCAACATTCAAAGTTAGAGAGTTTCAGGTTAATGAGAGCTTCCCATTTTCAATTTCCTTGTCCTGGAAAGGTTCTGGTCCAGACGCTCAGAACGGAGCAGCTGAGAACAACCAAAATACCCTTGTTTTCCCCAAGGGAAATCCTATCCCAAGTATCAAGGCCCTTACATTTTTCAGATCGGGCACTTTTTCAGTTGATGTGCAGTATGCTGATGTTAGTGATTTGCAGGCACCTGCAAAAATCAGCACATATACGATTGGTCCTTTCCAGTCTGTTAAAAGTGAGCGAGCAAAACTAAAGGTCAAAGCTCGCCTAAATCTGCATGGGATTGTGTCTGTAGACTCAGCAACTCTTTTGGAAGAAGAAGAAATTGAGGTTCCTGTCACAAAAGAGCAAACAAAGGAGGCTGCTAAGATGGAGACTGATGAGGCACCCAGTGATGTGGCTCCCCCAAGTACCAAAGAGACTGATGTGAGCATGCAAGATGCTAAGGACACTGCTGATGCTCCAGGTGCTGAAAATGTTGTTCCCGAGTCCGGGGAAAAGCCTGTGCAAATGGAAACTGATGCGAAGGCTGATGCTAAAAAGATAAAGGTAAGGAAAACAAACATTCCTGTGACAGAGTTAGTTTATGGAGGGATGCCACCAAATGAAGTGCAGAAGGCAATTGAGAAGGAGTTTGAAATGGCCTTACAAGACCGTGTTATGGAAGAAACAAAAGACAAGAAAAATGCTGTAGAGGCATACGTCTATGACATGAGAAACAAGCTCAATGACAAGTATCATGAATTTGTCACTGAATCTGAGAGGGAAGCGTTTATTGCTAGACTTCAGGAGGTGGAGGACTGGCTGTATGAAGATGGTGAAGACGAAACCAAAGGAGTTTACATTGCCAAACTTGAGGAGCTGAAGAAGCAAGGTGACCCCATTGAAGAGCGAGTCAAGGAGCATGCGGAGAGGGGAACAGTGATTGATCAACTTGCTTACTGTGTCAATAGTTACAGAGAAGCTGCAGCATCAAATGATGCAAAATTTGAACACATTGATCTGGCCGATAAACAGAAGGTATTGAACGAGTGTGTTGAAGCCGAGGCCTGGTTAAGAGAGAAAAAGCAGCAACAGGATCTGCTCCCCAAATATGCCAACCCAGTACTCTTATCAGCTGATGTGAGACGGAAGGCTGAAGCAGTTGACAGGTTCTGCAGACCAATAATGACGAAACCCAAACCAGCACCAGCCAAGCCAGCTGCTCCTGAAACTCCACCAACCCCACCTCCTCAGGGAAGTGAGCAACCTCAGGGCGGAGATGCAGATGCCAATCCCGGCCCCATGGAGAATCCTGCTGATGGCGACAATGAGGCCCCACAGGCTACTACAGAACCAATGGAAACCGACAAGCCAGAAGCTCCACAAAGTTCTGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0008144 GO:0008150 GO:0009266 GO:0009408 GO:0009506 GO:0009628 GO:0010035 GO:0010038 GO:0016020 GO:0017076 GO:0030054 GO:0030312 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0055044 GO:0071944 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04530 ko04612 map04530 map04612

Pfam Domains

Protein Families

HSP70 MreB_Mbl

Protein Analysis

856

Amino Acids

94.38

Weight (kDa)

5.17

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	3 - 695	2.9e-155	Hsp70 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79920 AT1G79920 AT1G79920 AT1G79920 AT1G79930 AT1G79930
fragaria_vesca	FvH4_6g31170 FvH4_6g31170 FvH4_6g31170 FvH4_6g31170
malus_domestica	MD09G1212600.v1.1 MD09G1213000.v1.1 MD09G1213700.v1.1
prunus_persica	Prupe.3G071300_v2.0.a1 Prupe.3G071300_v2.0.a1 Prupe.3G071300_v2.0.a1
pyrus_communis	pycom09g13020 pycom17g20100
rosa_chinensis	RchiOBHm_Chr2g0137851 RchiOBHm_Chr2g0137861
rosa_laevigata	RLG00000019681 RLG00000019684
rosa_multiflora	Rmu_sc0002170.1_g000008 Rmu_sc0002170.1_g000011 Rmu_sc0007548.1_g000005
rosa_roxburghii	Rroxscaffold_2G00106730 Rroxscaffold_2G00106760
rosa_rugosa	Rorug02G0347100 Rorug02G0347100 Rorug02G0347200 Rorug02G0347300
rosa_samantha	Rh2AG393500 Rh2AG393700 Rh2BG401000 Rh2CG380200 Rh2CG380400 Rh2DG416000
rosa_wichuraiana	Rw2G032230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1384
Acc36I	ACCTGC	1 cut(s)	1384
Acc65I	GGTACC	1 cut(s)	841
AccB1I	GGYRCC	3 cut(s)	841, 1372, 1573
AccB7I	CCANNNNNTGG	1 cut(s)	685
AccBSI	CCGCTC	1 cut(s)	36
AccI	GTMKAC	1 cut(s)	1482
AciI	CCGC	7 cut(s)	36, 136, 165, 798, 898, 2096, 2445
AclWI	GGATC	2 cut(s)	224, 2274
AcoI	YGGCCR	2 cut(s)	45, 2193
AcsI	RAATTY	4 cut(s)	734, 905, 1931, 2174
AcuI	CTGAAG	4 cut(s)	297, 1958, 2072, 2346
AdeI	CACNNNGTG	1 cut(s)	1580
AfaI	GTAC	6 cut(s)	711, 749, 796, 843, 1600, 2295
AflIII	ACRYGT	1 cut(s)	937
AjnI	CCWGG	4 cut(s)	53, 1205, 1651, 2237
AjuI	GAANNNNNNNTTGG	2 cut(s)	935, 967
AloI	GAACNNNNNNTCC	2 cut(s)	176, 208
Alw21I	GWGCWC	1 cut(s)	81
Alw26I	GTCTC	3 cut(s)	1556, 1601, 2308
AlwI	GGATC	2 cut(s)	224, 2274
AlwNI	CAGNNNCTG	4 cut(s)	530, 1658, 2269, 2343
Ama87I	CYCGRG	1 cut(s)	1673
ApoI	RAATTY	4 cut(s)	734, 905, 1931, 2174
Asp718I	GGTACC	1 cut(s)	841
AspA2I	CCTAGG	1 cut(s)	1076
AspS9I	GGNCC	9 cut(s)	109, 281, 947, 961, 1220, 1303, 1403, 2468, 2503
AsuC2I	CCSGG	2 cut(s)	1681, 2466
AsuHPI	GGTGA	3 cut(s)	137, 2018, 2075
AsuNHI	GCTAGC	1 cut(s)	611
AvaI	CYCGRG	1 cut(s)	1673
AvaII	GGWCC	3 cut(s)	947, 1220, 1403
AvrII	CCTAGG	1 cut(s)	1076
AxyI	CCTNAGG	2 cut(s)	2421, 2439
BaeGI	GKGCMC	1 cut(s)	1328
BanI	GGYRCC	3 cut(s)	841, 1372, 1573
BauI	CACGAG	1 cut(s)	1026
BbsI	GAAGAC	1 cut(s)	2016
Bbv12I	GWGCWC	1 cut(s)	81
BccI	CCATC	4 cut(s)	281, 1552, 1997, 2483
BceAI	ACGGC	1 cut(s)	32
BciT130I	CCWGG	4 cut(s)	55, 1207, 1653, 2239
BclI	TGATCA	1 cut(s)	2116
BcnI	CCSGG	2 cut(s)	1681, 2466
BcoDI	GTCTC	3 cut(s)	1556, 1601, 2308
BfaI	CTAG	3 cut(s)	612, 1077, 1968
BfmI	CTRYAG	6 cut(s)	522, 1479, 1878, 2154, 2344, 2517
BfuAI	ACCTGC	1 cut(s)	1384
BglI	GCCNNNNNGGC	1 cut(s)	2510
BlnI	CCTAGG	1 cut(s)	1076
BmcAI	AGTACT	2 cut(s)	711, 2295
Bme1390I	CCNGG	6 cut(s)	55, 1207, 1653, 1681, 2239, 2466
Bme18I	GGWCC	3 cut(s)	947, 1220, 1403
BmeT110I	CYCGRG	1 cut(s)	1673
BmgT120I	GGNCC	9 cut(s)	109, 281, 947, 961, 1220, 1303, 1403, 2468, 2503
BmiI	GGNNCC	9 cut(s)	843, 948, 1074, 1374, 1521, 1575, 1590, 2470, 2505
BmrFI	CCNGG	6 cut(s)	55, 1207, 1653, 1681, 2239, 2466
BmrI	ACTGGG	2 cut(s)	1572, 2285
BmtI	GCTAGC	1 cut(s)	615
BmuI	ACTGGG	2 cut(s)	1572, 2285
BpiI	GAAGAC	1 cut(s)	2016
BpmI	CTGGAG	1 cut(s)	1635
Bpu10I	CCTNAGC	1 cut(s)	1632
BpuEI	CTTGAG	1 cut(s)	2063
BpuMI	CCSGG	2 cut(s)	1681, 2466
BsaBI	GATNNNNATC	2 cut(s)	291, 2460
BsaJI	CCNNGG	5 cut(s)	1076, 1275, 1680, 2232, 2472
BsaXI	ACNNNNNCTCC	2 cut(s)	2401, 2431
Bse1I	ACTGG	6 cut(s)	434, 679, 1411, 1578, 1994, 2291
Bse21I	CCTNAGG	2 cut(s)	2421, 2439
Bse3DI	GCAATG	4 cut(s)	34, 627, 1133, 2031
Bse8I	GATNNNNATC	2 cut(s)	291, 2460
BseBI	CCWGG	4 cut(s)	55, 1207, 1653, 2239
BseDI	CCNNGG	5 cut(s)	1076, 1275, 1680, 2232, 2472
BseGI	GGATG	4 cut(s)	478, 883, 907, 1788
BseJI	GATNNNNATC	2 cut(s)	291, 2460
BseMI	GCAATG	4 cut(s)	34, 627, 1133, 2031
BseMII	CTCAG	6 cut(s)	1236, 1244, 1500, 1938, 2435, 2453
BseNI	ACTGG	6 cut(s)	434, 679, 1411, 1578, 1994, 2291
BseRI	GAGGAG	2 cut(s)	2060, 2409
BseSI	GKGCMC	1 cut(s)	1328
BseYI	CCCAGC	1 cut(s)	152
BsgI	GTGCAG	2 cut(s)	1364, 1820
BshNI	GGYRCC	3 cut(s)	841, 1372, 1573
BsiHKAI	GWGCWC	1 cut(s)	81
BsiHKCI	CYCGRG	1 cut(s)	1673
BsiSI	CCGG	3 cut(s)	107, 1680, 2466
BslFI	GGGAC	3 cut(s)	160, 710, 960
BsmAI	GTCTC	3 cut(s)	1556, 1601, 2308
BsmBI	CGTCTC	1 cut(s)	2308
BsmFI	GGGAC	3 cut(s)	160, 710, 960
BsmI	GAATGC	3 cut(s)	363, 775, 1096
BsoBI	CYCGRG	1 cut(s)	1673
Bsp1286I	GDGCHC	2 cut(s)	81, 1328
Bsp143I	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
Bsp19I	CCATGG	1 cut(s)	2472
BspACI	CCGC	7 cut(s)	36, 136, 165, 798, 898, 2096, 2445
BspCNI	CTCAG	6 cut(s)	1237, 1243, 1499, 1939, 2434, 2452
BspHI	TCATGA	1 cut(s)	1927
BspLI	GGNNCC	9 cut(s)	843, 948, 1074, 1374, 1521, 1575, 1590, 2470, 2505
BspMAI	CTGCAG	2 cut(s)	2158, 2348
BspMI	ACCTGC	1 cut(s)	1384
BspOI	GCTAGC	1 cut(s)	615
BspPI	GGATC	2 cut(s)	224, 2274
BspQI	GCTCTTC	2 cut(s)	454, 2070
BspT107I	GGYRCC	3 cut(s)	841, 1372, 1573
BsrBI	CCGCTC	1 cut(s)	36
BsrDI	GCAATG	4 cut(s)	34, 627, 1133, 2031
BsrI	ACTGG	6 cut(s)	434, 679, 1411, 1578, 1994, 2291
BssECI	CCNNGG	5 cut(s)	1076, 1275, 1680, 2232, 2472
BssMI	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
BssSI	CACGAG	1 cut(s)	1026
BssT1I	CCWWGG	3 cut(s)	1076, 1275, 2472
Bst2BI	CACGAG	1 cut(s)	1026
Bst2UI	CCWGG	4 cut(s)	55, 1207, 1653, 2239
Bst4CI	ACNGT	5 cut(s)	714, 1003, 1844, 2110, 2132
Bst6I	CTCTTC	5 cut(s)	454, 507, 738, 864, 2070
BstAPI	GCANNNNNTGC	1 cut(s)	485
BstDSI	CCRYGG	1 cut(s)	2472
BstEII	GGTNACC	1 cut(s)	2063
BstF5I	GGATG	4 cut(s)	478, 883, 907, 1788
BstKTI	GATC	7 cut(s)	232, 373, 451, 1322, 2119, 2191, 2269
BstMAI	GTCTC	3 cut(s)	1556, 1601, 2308
BstMBI	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
BstNI	CCWGG	4 cut(s)	55, 1207, 1653, 2239
BstNSI	RCATGY	4 cut(s)	611, 619, 1624, 2096
BstPI	GGTNACC	1 cut(s)	2063
BstSCI	CCNGG	6 cut(s)	53, 1205, 1651, 1679, 2237, 2464
BstSFI	CTRYAG	6 cut(s)	522, 1479, 1878, 2154, 2344, 2517
BstSLI	GKGCMC	1 cut(s)	1328
BstV2I	GAAGAC	1 cut(s)	2016
BstX2I	RGATCY	1 cut(s)	2266
BstXI	CCANNNNNNTGG	2 cut(s)	353, 1585
BstYI	RGATCY	1 cut(s)	2266
Bsu36I	CCTNAGG	2 cut(s)	2421, 2439
BtgI	CCRYGG	1 cut(s)	2472
BtsCI	GGATG	4 cut(s)	478, 883, 907, 1788
BtsI	GCAGTG	1 cut(s)	1638
BtsIMutI	CAGTG	5 cut(s)	441, 1585, 1638, 1938, 2115
BveI	ACCTGC	1 cut(s)	1384
CaiI	CAGNNNCTG	4 cut(s)	530, 1658, 2269, 2343
CciI	TCATGA	1 cut(s)	1927
Cfr13I	GGNCC	9 cut(s)	109, 281, 947, 961, 1220, 1303, 1403, 2468, 2503
CseI	GACGC	1 cut(s)	1235
Csp6I	GTAC	6 cut(s)	710, 748, 795, 842, 1599, 2294
CspCI	CAANNNNNGTGG	2 cut(s)	819, 854
CviQI	GTAC	6 cut(s)	710, 748, 795, 842, 1599, 2294
DpnI	GATC	7 cut(s)	231, 372, 450, 1321, 2118, 2190, 2268
DpnII	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
DraIII	CACNNNGTG	1 cut(s)	1580
EaeI	YGGCCR	2 cut(s)	45, 2193
Eam1104I	CTCTTC	5 cut(s)	454, 507, 738, 864, 2070
EarI	CTCTTC	5 cut(s)	454, 507, 738, 864, 2070
EciI	GGCGGA	1 cut(s)	2460
Eco130I	CCWWGG	3 cut(s)	1076, 1275, 2472
Eco147I	AGGCCT	1 cut(s)	2237
Eco32I	GATATC	2 cut(s)	250, 293
Eco47I	GGWCC	3 cut(s)	947, 1220, 1403
Eco57I	CTGAAG	4 cut(s)	297, 1958, 2072, 2346
Eco81I	CCTNAGG	2 cut(s)	2421, 2439
Eco88I	CYCGRG	1 cut(s)	1673
Eco91I	GGTNACC	1 cut(s)	2063
EcoO109I	RGGNCCY	5 cut(s)	281, 947, 961, 1303, 2503
EcoO65I	GGTNACC	1 cut(s)	2063
EcoRII	CCWGG	4 cut(s)	53, 1205, 1651, 2237
EcoRV	GATATC	2 cut(s)	250, 293
EcoT14I	CCWWGG	3 cut(s)	1076, 1275, 2472
ErhI	CCWWGG	3 cut(s)	1076, 1275, 2472
Esp3I	CGTCTC	1 cut(s)	2308
FaqI	GGGAC	3 cut(s)	160, 710, 960
FauI	CCCGC	1 cut(s)	891
FbaI	TGATCA	1 cut(s)	2116
FblI	GTMKAC	1 cut(s)	1482
FokI	GGATG	4 cut(s)	485, 890, 914, 1795
FspBI	CTAG	3 cut(s)	612, 1077, 1968
GsaI	CCCAGC	1 cut(s)	156
GsuI	CTGGAG	1 cut(s)	1635
HapII	CCGG	3 cut(s)	107, 1680, 2466
HgaI	GACGC	1 cut(s)	1235
HincII	GTYRAC	1 cut(s)	2335
HindII	GTYRAC	1 cut(s)	2335
HinfI	GANTC	9 cut(s)	89, 301, 394, 430, 1484, 1676, 1943, 2082, 2479
HpaII	CCGG	3 cut(s)	107, 1680, 2466
HphI	GGTGA	3 cut(s)	137, 2018, 2075
Hpy166II	GTNNAC	3 cut(s)	1483, 2029, 2335
Hpy188I	TCNGA	7 cut(s)	229, 277, 370, 375, 1233, 1319, 1948
Hpy188III	TCNNGA	8 cut(s)	640, 968, 1045, 1223, 1673, 1928, 1976, 2396
Hpy8I	GTNNAC	3 cut(s)	1483, 2029, 2335
HpyCH4III	ACNGT	5 cut(s)	714, 1003, 1844, 2110, 2132
HpyCH4IV	ACGT	4 cut(s)	72, 97, 937, 1890
HpySE526I	ACGT	4 cut(s)	72, 97, 937, 1890
KpnI	GGTACC	1 cut(s)	845
Ksp22I	TGATCA	1 cut(s)	2116
Kzo9I	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
LguI	GCTCTTC	2 cut(s)	454, 2070
LmnI	GCTCC	9 cut(s)	1072, 1238, 1594, 1654, 2047, 2089, 2277, 2398, 2554
MaeI	CTAG	3 cut(s)	612, 1077, 1968
MaeII	ACGT	4 cut(s)	72, 97, 937, 1890
MaeIII	GTNAC	6 cut(s)	125, 1525, 1759, 1936, 2063, 2141
MalI	GATC	7 cut(s)	231, 372, 450, 1321, 2118, 2190, 2268
MbiI	CCGCTC	1 cut(s)	36
MboI	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
MfeI	CAATTG	2 cut(s)	578, 1809
MflI	RGATCY	1 cut(s)	2266
MhlI	GDGCHC	2 cut(s)	81, 1328
MlyI	GAGTC	4 cut(s)	98, 1478, 1685, 2091
MmeI	TCCRAC	2 cut(s)	478, 1169
MseI	TTAA	6 cut(s)	201, 863, 894, 1173, 1419, 2243
MslI	CAYNNNNRTG	1 cut(s)	1757
MspA1I	CMGCKG	7 cut(s)	136, 152, 407, 652, 1244, 2306, 2390
MspI	CCGG	3 cut(s)	107, 1680, 2466
MspR9I	CCNGG	6 cut(s)	55, 1207, 1653, 1681, 2239, 2466
MunI	CAATTG	2 cut(s)	578, 1809
Mva1269I	GAATGC	3 cut(s)	363, 775, 1096
MvaI	CCWGG	4 cut(s)	55, 1207, 1653, 2239
NciI	CCSGG	2 cut(s)	1681, 2466
NcoI	CCATGG	1 cut(s)	2472
NdeII	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
NheI	GCTAGC	1 cut(s)	611
NlaIV	GGNNCC	9 cut(s)	843, 948, 1074, 1374, 1521, 1575, 1590, 2470, 2505
NmeAIII	GCCGAG	2 cut(s)	412, 2257
NmuCI	GTSAC	5 cut(s)	125, 1525, 1759, 1936, 2063
NspI	RCATGY	4 cut(s)	611, 619, 1624, 2096
PaeI	GCATGC	4 cut(s)	611, 619, 1624, 2096
PagI	TCATGA	1 cut(s)	1927
PaqCI	CACCTGC	1 cut(s)	1384
PceI	AGGCCT	1 cut(s)	2237
PciSI	GCTCTTC	2 cut(s)	454, 2070
PctI	GAATGC	3 cut(s)	363, 775, 1096
PfeI	GAWTC	5 cut(s)	301, 394, 430, 1943, 2479
PflMI	CCANNNNNTGG	1 cut(s)	685
PfoI	TCCNGGA	1 cut(s)	1205
PleI	GAGTC	4 cut(s)	97, 1478, 1684, 2090
PpsI	GAGTC	4 cut(s)	97, 1478, 1684, 2090
PpuMI	RGGWCCY	1 cut(s)	947
Psp5II	RGGWCCY	1 cut(s)	947
Psp6I	CCWGG	4 cut(s)	53, 1205, 1651, 2237
PspEI	GGTNACC	1 cut(s)	2063
PspFI	CCCAGC	1 cut(s)	152
PspGI	CCWGG	4 cut(s)	53, 1205, 1651, 2237
PspN4I	GGNNCC	9 cut(s)	843, 948, 1074, 1374, 1521, 1575, 1590, 2470, 2505
PspPI	GGNCC	9 cut(s)	109, 281, 947, 961, 1220, 1303, 1403, 2468, 2503
PspPPI	RGGWCCY	1 cut(s)	947
PstI	CTGCAG	2 cut(s)	2158, 2348
PstNI	CAGNNNCTG	4 cut(s)	530, 1658, 2269, 2343
PsuI	RGATCY	1 cut(s)	2266
PvuII	CAGCTG	6 cut(s)	152, 407, 652, 1244, 2306, 2390
RsaI	GTAC	6 cut(s)	711, 749, 796, 843, 1600, 2295
RsaNI	GTAC	6 cut(s)	710, 748, 795, 842, 1599, 2294
RseI	CAYNNNNRTG	1 cut(s)	1757
SapI	GCTCTTC	2 cut(s)	454, 2070
SaqAI	TTAA	6 cut(s)	201, 863, 894, 1173, 1419, 2243
Sau3AI	GATC	7 cut(s)	229, 370, 448, 1319, 2116, 2188, 2266
Sau96I	GGNCC	9 cut(s)	109, 281, 947, 961, 1220, 1303, 1403, 2468, 2503
ScaI	AGTACT	2 cut(s)	711, 2295
SchI	GAGTC	4 cut(s)	98, 1478, 1685, 2091
ScrFI	CCNGG	6 cut(s)	55, 1207, 1653, 1681, 2239, 2466
SduI	GDGCHC	2 cut(s)	81, 1328
SfcI	CTRYAG	6 cut(s)	522, 1479, 1878, 2154, 2344, 2517
SinI	GGWCC	3 cut(s)	947, 1220, 1403
SmiMI	CAYNNNNRTG	1 cut(s)	1757
SmlI	CTYRAG	1 cut(s)	2042
SmoI	CTYRAG	1 cut(s)	2042
SphI	GCATGC	4 cut(s)	611, 619, 1624, 2096
SseBI	AGGCCT	1 cut(s)	2237
SsiI	CCGC	7 cut(s)	36, 136, 165, 798, 898, 2096, 2445
SspI	AATATT	1 cut(s)	660
SspMI	CTAG	3 cut(s)	612, 1077, 1968
StuI	AGGCCT	1 cut(s)	2237
StyD4I	CCNGG	6 cut(s)	53, 1205, 1651, 1679, 2237, 2464
StyI	CCWWGG	3 cut(s)	1076, 1275, 2472
TaaI	ACNGT	5 cut(s)	714, 1003, 1844, 2110, 2132
TaiI	ACGT	4 cut(s)	75, 100, 940, 1893
TaqI	TCGA	1 cut(s)	414
TatI	WGTACW	3 cut(s)	709, 747, 2293
TauI	GCSGC	1 cut(s)	39
TfiI	GAWTC	5 cut(s)	301, 394, 430, 1943, 2479
Tru1I	TTAA	6 cut(s)	201, 863, 894, 1173, 1419, 2243
Tru9I	TTAA	6 cut(s)	201, 863, 894, 1173, 1419, 2243
TscAI	CASTG	5 cut(s)	441, 1585, 1645, 1945, 2115
TseFI	GTSAC	5 cut(s)	125, 1525, 1759, 1936, 2063
Tsp45I	GTSAC	5 cut(s)	125, 1525, 1759, 1936, 2063
TspGWI	ACGGA	3 cut(s)	572, 1251, 2332
TspRI	CASTG	5 cut(s)	441, 1585, 1645, 1945, 2115
Van91I	CCANNNNNTGG	1 cut(s)	685
VpaK11BI	GGWCC	3 cut(s)	947, 1220, 1403
XapI	RAATTY	4 cut(s)	734, 905, 1931, 2174
XceI	RCATGY	4 cut(s)	611, 619, 1624, 2096
XmaJI	CCTAGG	1 cut(s)	1076
XmiI	GTMKAC	1 cut(s)	1482
XspI	CTAG	3 cut(s)	612, 1077, 1968
ZrmI	AGTACT	2 cut(s)	711, 2295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.