Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009747

Physical Location & Seq

Genomic Coordinates Forward (+)

26465960 .. 26471333

Gene Structure 5374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1223600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGGCGGAAGGCAAAGCAATTAGCTTGGAGGAAATCAAGAAGGAAAATGTCGACCTGGAGCGCATTCCAGTTCAGGAAGTGTTCGAACAATTGAATTGCACAAAAGAGGGTTTGTCAAGCGATGAAGGGCAGAAGAGGCTTCAGATCTTCGGGCCAAACAAGCTCGAAGAAAAGAAGGATAACAAAGTGCTCAAGTTTTTGGGTTTTATGTGGAATCCCCTATCGTGGGTGATGGAGGCTGCTGCAATCATGGCCATTGCCTTGGCAAATGGAGGGGGAAGACCACCAGACTGGCAGGACTTTGTGGGAATTACTGCTCTCTTGATCATCAACTCTACCATAAGCTTTATCGAAGAAAACAATGCAGGAAATGCTGCAGCGGCATTGATGGCGGGTCTTGCACCGAAAACAAAGGTTCTGAGAGATGGAAGATGGAATGAGCTAGAAGCTGCAATCTTGGTACCAGGAGATGTGGTCAGTATTAAGTTGGGAGATATTGTCCCTGCAGATGCTCGTCTCTTGGACGGAGATCCTCTAAAGATCGATCAATCTGCCCTCACTGGTGAATCTCTGCCCGTGACAAGGTACCCTGGCGATGAAGTTTTCTCTGGCTCGACCGTCAAGCAAGGTGAGATTGAGGCAGTTGTAATTGCCACCGGTGTTCATACCTTCTTTGGGAAGGCTGCTCACCTTGTTGATAGCACCAACCAAGTAGGCCACTTCCAAAAGGTTTTGACTGCCATTGGAAACTTCTGCATATGCTCCATTGGCGTGGGAATTGTTATTGAGGTAATAGTCATGTATCCAATTCAGCACCGGGCGTACAGAGATGGAATTGACAATCTCCTGGTGCTTCTCATTGGAGGCATTCCAATTGCTATGCCAACGGTGTTGTCAGTTACGATGGCAATTGGTTCCCACCGCCTCTCGACACAAGGTGCCATCACCAAGAGGATGACAGCCATTGAGGAGATGGCTGGAATGGATGTCCTCTGCAGTGATAAGACTGGAACTCTTACTCTTAACAAGCTTACAGTTGACAAGACCATGGTTGAGGTGTTTGTAAAGGATGTGGACAAGGATGGCCTAATTCTGCTCGGAGCCAGGGCTTCCAGGGTTGAGAATCAGGACGCCATTGACACCTGCATCGTTGGAATGCTGGGAGACCCCAAGGAGGCCAGGGAAGGAATAAATGAGGTCCATTTCTTGCCCTTTAATCCAGTGGAAAAACGTACCGCCATAACATACATCGACCCTGAAGGGAATTGGCATCGGGTTAGCAAAGGTGCACCAGAGCAGATCATTGAGCTTTGTAACCTAAAGGGTGATGTTGAGAAGAAAGCACATGCTATCATTGGCAAGTTTGCTGATCGCGGCCTTCGCTCCCTTGCTGTTGCTAGACAGACCGTGCCGGAAAAAAACAAAGAGAGTGCTGGAACACCATGGGAGTTTGTGGGTCTCTTGCCTCTCTTTGATCCTCCAAGGCATGACAGTGCAGAGACCATTCGCCGAGCCCTTAATCTCGGTGTCAATGTCAAGATGATCACTGGTGATCAGCTTGCCATTGGCAAGGAGACCGGCCGCAGACTTGGCATGGGCACCAACATGTATCCTTCTTCTTCCCTCCTTGGTGACACAAAGGACGAGTCGATCGCTAGCCTCCCTATTGATGAGCTTATCGAAAAGGCTGATGGCTTTGCTGGTGTCTTCCCAGAGCATAAGTATGAAATTGTCAAGAGGCTGCAAGACAGGAAGCATATTTGTGGGATGACTGGAGATGGTGTGAATGATGCCCCAGCTCTAAAGAGGGCAGACATTGGAATTGCAGTGGATGATGCAACTGATGCAGCTCGCAGCGCATCCGACATTGTCCTGACAGAACCAGGGTTGTCTGTGATCATCAGTGCTGTGTTGACGAGCAGAGCCATCTTCCAGAGGATGAAGAATTACACCATTTATGCCGTTTCCATAACAATCCGTGTTGTGCTAGGTTTTATGCTCCTTGCTCTCATCTGGAAGTTTGATTTCTCGCCTTTCATGGTTCTGGTTATCGCCATACTCAATGACGGAACAATCATGACTATTTCTAAAGATAGGGTGAAGCCATCTCCTCTTCCAGACTCATGGAAGCTAAAGGAGATCTTCGCGACTGGCGTAGTCCTTGGCACCTACATGGCTGTCATGACTGTTGTTTTCTTCTGGGCGGCTCATGGCTCTGACTTTTTCACGGAAAAATTTGGGGTAAGATCTATCAGGGGAAACTTTCCTGAGCTTACAGCAGCTATCTACCTTCAAGTGAGTATTATTAGTCAGGCACTTATCTTTGTTACTCGATCGCGAAGCTGGTCTTATGTTGAACGCCCCGGTTTCTTTCTCTTGGGAGCCTTTGTCATAGCACAGCTGATTGCTACCATAATTGCTGTTTATGCTAACTGGGGCTTTGCAAGAATCCATGGCATTGGATGGGGATGGGCAGGAGTTATCTGGCTCTACAGCCTTATCACCTACGTACCATTGGACATTCTCAAGTTCATCATTCGATACGTGTTGAGCGGCAAGGCCTGGGATAATGTACTTGAGAGAAAGACTGCTTTCACAACAAAGAAGGATTACGGAAAGGGAGAGAGGGAGGCGCAATGGGCTACCGCTCAGCGAACCCTCCACGGTCTGCAGCCTCCTGAGACATCTGAGCTCTTTAATGACAAGAACAACTACAGAGAGTTGTCTGAAATCGCTGAACAGGCCAAGAGGCGAGCTGAAGTTGCTAGGTTGAGGGAGCTTCACACACTCAAGGGGCACGTCGAGTCGGTGGTGAAGCTTAAAGGACTAGACATTGACACCATTCAACAACACTACACTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008553 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031090 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

955

Amino Acids

104.84

Weight (kDa)

6.17

Isoelectric Point (pI)

32.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	21 - 84	1.8e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	134 - 232	9.8e-30	P-type ATPase actuator domain
Hydrolase	PF00702	328 - 605	1.2e-13	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009747)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80660 AT1G80660 AT1G80660 AT1G80660
fragaria_vesca	FvH4_6g28890
malus_domestica	MD09G1223600.v1.1
prunus_persica	Prupe.3G095900_v2.0.a1 Prupe.3G095900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0133471
rosa_laevigata	RLG00000019336
rosa_multiflora	Rmu_sc0004887.1_g000004 Rmu_sc0007236.1_g000003
rosa_rugosa	Rorug02G0311900 Rorug02G0311900 Rorug02G0311900 Rorug02G0312000
rosa_samantha	Rh2AG364500 Rh2BG370200 Rh2CG347800 Rh2DG387200
rosa_wichuraiana	Rw2G029710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1151
Acc36I	ACCTGC	1 cut(s)	1151
Acc65I	GGTACC	2 cut(s)	460, 585
AccB1I	GGYRCC	5 cut(s)	460, 585, 938, 1598, 2165
AccBSI	CCGCTC	2 cut(s)	2553, 2648
AccI	GTMKAC	1 cut(s)	51
AccII	CGCG	3 cut(s)	1374, 2147, 2338
AclWI	GGATC	2 cut(s)	524, 1469
AcoI	YGGCCR	2 cut(s)	252, 1579
AcsI	RAATTY	1 cut(s)	2234
AcuI	CTGAAG	3 cut(s)	125, 1278, 2778
AcyI	GRCGYC	1 cut(s)	1131
AdeI	CACNNNGTG	1 cut(s)	938
AfaI	GTAC	6 cut(s)	462, 587, 824, 1234, 2511, 2574
AfiI	CCNNNNNNNGG	5 cut(s)	225, 226, 582, 675, 1174
AflIII	ACRYGT	2 cut(s)	1605, 2544
AgeI	ACCGGT	1 cut(s)	656
AgsI	TTSAA	4 cut(s)	94, 2294, 2357, 2846
AjiI	CACGTC	1 cut(s)	2800
AjnI	CCWGG	9 cut(s)	54, 463, 589, 846, 1103, 1112, 1178, 1882, 2561
Alw21I	GWGCWC	3 cut(s)	192, 1291, 2694
Alw26I	GTCTC	6 cut(s)	521, 1158, 1463, 1493, 1568, 2675
Alw44I	GTGCAC	1 cut(s)	1287
AlwI	GGATC	2 cut(s)	524, 1469
AoxI	GGCC	9 cut(s)	152, 252, 715, 1084, 1176, 1375, 1579, 2559, 2742
ApaLI	GTGCAC	1 cut(s)	1287
ApoI	RAATTY	1 cut(s)	2234
ArsI	GACNNNNNNTTYG	2 cut(s)	1631, 1663
AsiGI	ACCGGT	1 cut(s)	656
Asp718I	GGTACC	2 cut(s)	460, 585
AspLEI	GCGC	3 cut(s)	63, 1859, 2635
AspS9I	GGNCC	2 cut(s)	152, 1198
AsuC2I	CCSGG	2 cut(s)	818, 2364
AsuII	TTCGAA	1 cut(s)	84
AsuNHI	GCTAGC	1 cut(s)	1655
AvaII	GGWCC	1 cut(s)	1198
BaeGI	GKGCMC	3 cut(s)	1291, 1601, 2799
BaeI	ACNNNNGTAYC	2 cut(s)	2493, 2526
BalI	TGGCCA	1 cut(s)	254
BanI	GGYRCC	5 cut(s)	460, 585, 938, 1598, 2165
BanII	GRGCYC	2 cut(s)	1516, 2694
BbsI	GAAGAC	2 cut(s)	286, 1699
Bbv12I	GWGCWC	3 cut(s)	192, 1291, 2694
BceAI	ACGGC	1 cut(s)	1946
BcgI	CGANNNNNNTGC	2 cut(s)	533, 567
BciT130I	CCWGG	9 cut(s)	56, 465, 591, 848, 1105, 1114, 1180, 1884, 2563
BciVI	GTATCC	2 cut(s)	813, 1620
BclI	TGATCA	4 cut(s)	324, 1542, 1552, 1896
BcnI	CCSGG	2 cut(s)	818, 2364
BcoDI	GTCTC	6 cut(s)	521, 1158, 1463, 1493, 1568, 2675
BfaI	CTAG	6 cut(s)	443, 1398, 1656, 1988, 2766, 2828
BfmI	CTRYAG	6 cut(s)	375, 504, 994, 2491, 2669, 2713
BfuAI	ACCTGC	1 cut(s)	1151
BfuI	GTATCC	2 cut(s)	813, 1620
BglII	AGATCT	3 cut(s)	144, 2139, 2246
BlpI	GCTNAGC	1 cut(s)	2649
Bme18I	GGWCC	1 cut(s)	1198
BmgBI	CACGTC	1 cut(s)	2800
BmgT120I	GGNCC	2 cut(s)	152, 1198
BmiI	GGNNCC	8 cut(s)	462, 587, 916, 940, 1102, 1600, 2167, 2383
BmrI	ACTGGG	1 cut(s)	2443
BmsI	GCATC	7 cut(s)	499, 1155, 1279, 1780, 1825, 1834, 1868
BmtI	GCTAGC	1 cut(s)	1659
BmuI	ACTGGG	1 cut(s)	2443
BpiI	GAAGAC	2 cut(s)	286, 1699
BpmI	CTGGAG	2 cut(s)	77, 1794
Bpu10I	CCTNAGC	1 cut(s)	2268
Bpu1102I	GCTNAGC	1 cut(s)	2649
Bpu14I	TTCGAA	1 cut(s)	84
BpuEI	CTTGAG	4 cut(s)	176, 2510, 2597, 2774
BpuMI	CCSGG	2 cut(s)	818, 2364
Bsa29I	ATCGAT	1 cut(s)	543
BsaAI	YACGTR	2 cut(s)	2509, 2545
BsaHI	GRCGYC	1 cut(s)	1131
BsaI	GGTCTC	4 cut(s)	1158, 1463, 1493, 1568
BsaWI	WCCGGW	1 cut(s)	656
BsaXI	ACNNNNNCTCC	8 cut(s)	459, 483, 489, 513, 1767, 1797, 2373, 2403
Bsc4I	CCNNNNNNNGG	5 cut(s)	225, 226, 582, 675, 1174
Bse118I	RCCGGY	2 cut(s)	656, 1577
Bse1I	ACTGG	9 cut(s)	68, 296, 565, 1012, 1220, 1552, 1777, 2155, 2438
Bse3DI	GCAATG	2 cut(s)	255, 2642
BseBI	CCWGG	9 cut(s)	56, 465, 591, 848, 1105, 1114, 1180, 1884, 2563
BseCI	ATCGAT	1 cut(s)	543
BseLI	CCNNNNNNNGG	5 cut(s)	225, 226, 582, 675, 1174
BseMI	GCAATG	2 cut(s)	255, 2642
BseMII	CTCAG	5 cut(s)	410, 2259, 2663, 2670, 2679
BseNI	ACTGG	9 cut(s)	68, 296, 565, 1012, 1220, 1552, 1777, 2155, 2438
BseRI	GAGGAG	2 cut(s)	983, 2100
BseSI	GKGCMC	3 cut(s)	1291, 1601, 2799
BseX3I	CGGCCG	1 cut(s)	1579
BseYI	CCCAGC	2 cut(s)	1159, 1795
BsgI	GTGCAG	1 cut(s)	1515
Bsh1236I	CGCG	3 cut(s)	1374, 2147, 2338
Bsh1285I	CGRYCG	4 cut(s)	618, 1582, 1653, 2336
BshFI	GGCC	9 cut(s)	154, 254, 717, 1086, 1178, 1377, 1581, 2561, 2744
BshNI	GGYRCC	5 cut(s)	460, 585, 938, 1598, 2165
BshTI	ACCGGT	1 cut(s)	656
BshVI	ATCGAT	1 cut(s)	543
BsiEI	CGRYCG	4 cut(s)	618, 1582, 1653, 2336
BsiHKAI	GWGCWC	3 cut(s)	192, 1291, 2694
BsiSI	CCGG	5 cut(s)	657, 817, 1412, 1578, 2364
BslFI	GGGAC	1 cut(s)	485
BslI	CCNNNNNNNGG	5 cut(s)	225, 226, 582, 675, 1174
BsmAI	GTCTC	6 cut(s)	521, 1158, 1463, 1493, 1568, 2675
BsmBI	CGTCTC	1 cut(s)	521
BsmFI	GGGAC	1 cut(s)	485
BsmI	GAATGC	3 cut(s)	63, 867, 1161
BsnI	GGCC	9 cut(s)	154, 254, 717, 1086, 1178, 1377, 1581, 2561, 2744
Bso31I	GGTCTC	4 cut(s)	1158, 1463, 1493, 1568
Bsp119I	TTCGAA	1 cut(s)	84
Bsp1286I	GDGCHC	6 cut(s)	192, 1291, 1516, 1601, 2694, 2799
Bsp1720I	GCTNAGC	1 cut(s)	2649
Bsp19I	CCATGG	3 cut(s)	1047, 1442, 2452
Bsp68I	TCGCGA	2 cut(s)	2147, 2338
BspANI	GGCC	9 cut(s)	154, 254, 717, 1086, 1178, 1377, 1581, 2561, 2744
BspCNI	CTCAG	5 cut(s)	411, 2260, 2662, 2671, 2680
BspDI	ATCGAT	1 cut(s)	543
BspFNI	CGCG	3 cut(s)	1374, 2147, 2338
BspHI	TCATGA	2 cut(s)	2076, 2181
BspLI	GGNNCC	8 cut(s)	462, 587, 916, 940, 1102, 1600, 2167, 2383
BspMAI	CTGCAG	4 cut(s)	379, 508, 998, 2673
BspMI	ACCTGC	1 cut(s)	1151
BspOI	GCTAGC	1 cut(s)	1659
BspPI	GGATC	2 cut(s)	524, 1469
BspT104I	TTCGAA	1 cut(s)	84
BspT107I	GGYRCC	5 cut(s)	460, 585, 938, 1598, 2165
BspTNI	GGTCTC	4 cut(s)	1158, 1463, 1493, 1568
BsrBI	CCGCTC	2 cut(s)	2553, 2648
BsrDI	GCAATG	2 cut(s)	255, 2642
BsrFI	RCCGGY	2 cut(s)	656, 1577
BsrI	ACTGG	9 cut(s)	68, 296, 565, 1012, 1220, 1552, 1777, 2155, 2438
BssAI	RCCGGY	2 cut(s)	656, 1577
BssNI	GRCGYC	1 cut(s)	1131
BssT1I	CCWWGG	8 cut(s)	261, 1047, 1170, 1442, 1481, 1627, 2161, 2452
Bst2UI	CCWGG	9 cut(s)	56, 465, 591, 848, 1105, 1114, 1180, 1884, 2563
Bst4CI	ACNGT	8 cut(s)	619, 889, 1036, 1408, 1493, 2188, 2666, 2860
Bst6I	CTCTTC	2 cut(s)	128, 2118
BstACI	GRCGYC	1 cut(s)	1131
BstAPI	GCANNNNNTGC	2 cut(s)	371, 1844
BstBAI	YACGTR	2 cut(s)	2509, 2545
BstBI	TTCGAA	1 cut(s)	84
BstC8I	GCNNGC	4 cut(s)	1560, 1657, 1852, 2754
BstDEI	CTNAG	5 cut(s)	419, 2268, 2649, 2679, 2688
BstDSI	CCRYGG	4 cut(s)	1047, 1442, 2452, 2662
BstFNI	CGCG	3 cut(s)	1374, 2147, 2338
BstHHI	GCGC	3 cut(s)	63, 1859, 2635
BstMAI	GTCTC	6 cut(s)	521, 1158, 1463, 1493, 1568, 2675
BstMCI	CGRYCG	4 cut(s)	618, 1582, 1653, 2336
BstNI	CCWGG	9 cut(s)	56, 465, 591, 848, 1105, 1114, 1180, 1884, 2563
BstNSI	RCATGY	2 cut(s)	1349, 1609
BstSFI	CTRYAG	6 cut(s)	375, 504, 994, 2491, 2669, 2713
BstSLI	GKGCMC	3 cut(s)	1291, 1601, 2799
BstSNI	TACGTA	1 cut(s)	2509
BstUI	CGCG	3 cut(s)	1374, 2147, 2338
BstV2I	GAAGAC	2 cut(s)	286, 1699
BstX2I	RGATCY	4 cut(s)	144, 529, 2139, 2246
BstXI	CCANNNNNNTGG	5 cut(s)	262, 291, 772, 2124, 2459
BstYI	RGATCY	4 cut(s)	144, 529, 2139, 2246
BstZI	CGGCCG	1 cut(s)	1579
Bsu15I	ATCGAT	1 cut(s)	543
BsuI	GTATCC	2 cut(s)	813, 1620
BsuRI	GGCC	9 cut(s)	154, 254, 717, 1086, 1178, 1377, 1581, 2561, 2744
BsuTUI	ATCGAT	1 cut(s)	543
BtgI	CCRYGG	4 cut(s)	1047, 1442, 2452, 2662
BtgZI	GCGATG	2 cut(s)	135, 609
BtrI	CACGTC	1 cut(s)	2800
BtsI	GCAGTG	2 cut(s)	1003, 1833
BtsIMutI	CAGTG	8 cut(s)	558, 1003, 1227, 1498, 1545, 1833, 1909, 2856
BtuMI	TCGCGA	2 cut(s)	2147, 2338
BveI	ACCTGC	1 cut(s)	1151
Cac8I	GCNNGC	4 cut(s)	1560, 1657, 1852, 2754
CciI	TCATGA	2 cut(s)	2076, 2181
CfoI	GCGC	3 cut(s)	63, 1859, 2635
Cfr10I	RCCGGY	2 cut(s)	656, 1577
Cfr13I	GGNCC	2 cut(s)	152, 1198
ClaI	ATCGAT	1 cut(s)	543
CseI	GACGC	1 cut(s)	1139
Csp6I	GTAC	6 cut(s)	461, 586, 823, 1233, 2510, 2573
CspAI	ACCGGT	1 cut(s)	656
CviQI	GTAC	6 cut(s)	461, 586, 823, 1233, 2510, 2573
DdeI	CTNAG	5 cut(s)	419, 2268, 2649, 2679, 2688
DraIII	CACNNNGTG	1 cut(s)	938
EaeI	YGGCCR	2 cut(s)	252, 1579
EagI	CGGCCG	1 cut(s)	1579
Eam1104I	CTCTTC	2 cut(s)	128, 2118
EarI	CTCTTC	2 cut(s)	128, 2118
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	2692
EclXI	CGGCCG	1 cut(s)	1579
Eco105I	TACGTA	1 cut(s)	2509
Eco130I	CCWWGG	8 cut(s)	261, 1047, 1170, 1442, 1481, 1627, 2161, 2452
Eco147I	AGGCCT	1 cut(s)	2561
Eco24I	GRGCYC	2 cut(s)	1516, 2694
Eco31I	GGTCTC	4 cut(s)	1158, 1463, 1493, 1568
Eco47I	GGWCC	1 cut(s)	1198
Eco52I	CGGCCG	1 cut(s)	1579
Eco53kI	GAGCTC	1 cut(s)	2692
Eco57I	CTGAAG	3 cut(s)	125, 1278, 2778
EcoICRI	GAGCTC	1 cut(s)	2692
EcoRII	CCWGG	9 cut(s)	54, 463, 589, 846, 1103, 1112, 1178, 1882, 2561
EcoT14I	CCWWGG	8 cut(s)	261, 1047, 1170, 1442, 1481, 1627, 2161, 2452
EcoT38I	GRGCYC	2 cut(s)	1516, 2694
ErhI	CCWWGG	8 cut(s)	261, 1047, 1170, 1442, 1481, 1627, 2161, 2452
Esp3I	CGTCTC	1 cut(s)	521
FalI	AAGNNNNNCTT	4 cut(s)	2126, 2158, 2332, 2364
FaqI	GGGAC	1 cut(s)	485
FauI	CCCGC	1 cut(s)	385
FauNDI	CATATG	1 cut(s)	758
FbaI	TGATCA	4 cut(s)	324, 1542, 1552, 1896
FblI	GTMKAC	1 cut(s)	51
FriOI	GRGCYC	2 cut(s)	1516, 2694
FspBI	CTAG	6 cut(s)	443, 1398, 1656, 1988, 2766, 2828
GlaI	GCGC	3 cut(s)	62, 1858, 2634
GsaI	CCCAGC	2 cut(s)	1163, 1799
GsuI	CTGGAG	2 cut(s)	77, 1794
HaeIII	GGCC	9 cut(s)	154, 254, 717, 1086, 1178, 1377, 1581, 2561, 2744
HapII	CCGG	5 cut(s)	657, 817, 1412, 1578, 2364
HgaI	GACGC	1 cut(s)	1139
HhaI	GCGC	3 cut(s)	63, 1859, 2635
Hin1I	GRCGYC	1 cut(s)	1131
Hin6I	GCGC	3 cut(s)	61, 1857, 2633
HinP1I	GCGC	3 cut(s)	61, 1857, 2633
HincII	GTYRAC	3 cut(s)	52, 1039, 1914
HindII	GTYRAC	3 cut(s)	52, 1039, 1914
HindIII	AAGCTT	3 cut(s)	343, 1028, 2816
HinfI	GANTC	7 cut(s)	214, 566, 1123, 1646, 2120, 2448, 2804
HpaII	CCGG	5 cut(s)	657, 817, 1412, 1578, 2364
Hpy166II	GTNNAC	5 cut(s)	52, 1039, 1075, 1289, 1914
Hpy188I	TCNGA	7 cut(s)	144, 420, 1100, 1864, 2218, 2689, 2728
Hpy8I	GTNNAC	5 cut(s)	52, 1039, 1075, 1289, 1914
Hpy99I	CGWCG	1 cut(s)	2804
HpyCH4III	ACNGT	8 cut(s)	619, 889, 1036, 1408, 1493, 2188, 2666, 2860
HpyCH4IV	ACGT	4 cut(s)	1231, 2508, 2544, 2799
HpyF3I	CTNAG	5 cut(s)	419, 2268, 2649, 2679, 2688
HpySE526I	ACGT	4 cut(s)	1231, 2508, 2544, 2799
Hsp92I	GRCGYC	1 cut(s)	1131
HspAI	GCGC	3 cut(s)	61, 1857, 2633
KpnI	GGTACC	2 cut(s)	464, 589
Ksp22I	TGATCA	4 cut(s)	324, 1542, 1552, 1896
LmnI	GCTCC	7 cut(s)	58, 767, 1100, 1388, 2004, 2381, 2776
LweI	GCATC	7 cut(s)	499, 1155, 1279, 1780, 1825, 1834, 1868
MaeI	CTAG	6 cut(s)	443, 1398, 1656, 1988, 2766, 2828
MaeII	ACGT	4 cut(s)	1231, 2508, 2544, 2799
MaeIII	GTNAC	5 cut(s)	577, 898, 1313, 1631, 2326
MbiI	CCGCTC	2 cut(s)	2553, 2648
MfeI	CAATTG	3 cut(s)	89, 873, 909
MflI	RGATCY	4 cut(s)	144, 529, 2139, 2246
MhlI	GDGCHC	6 cut(s)	192, 1291, 1516, 1601, 2694, 2799
MlsI	TGGCCA	1 cut(s)	254
MluNI	TGGCCA	1 cut(s)	254
MlyI	GAGTC	3 cut(s)	1655, 2114, 2813
MmeI	TCCRAC	2 cut(s)	1132, 1887
Mox20I	TGGCCA	1 cut(s)	254
MscI	TGGCCA	1 cut(s)	254
MseI	TTAA	7 cut(s)	483, 1023, 1215, 1518, 2697, 2820, 2863
MslI	CAYNNNNRTG	6 cut(s)	770, 1491, 1604, 1722, 1761, 2171
Msp20I	TGGCCA	1 cut(s)	254
MspA1I	CMGCKG	2 cut(s)	380, 2401
MspI	CCGG	5 cut(s)	657, 817, 1412, 1578, 2364
MunI	CAATTG	3 cut(s)	89, 873, 909
Mva1269I	GAATGC	3 cut(s)	63, 867, 1161
MvaI	CCWGG	9 cut(s)	56, 465, 591, 848, 1105, 1114, 1180, 1884, 2563
MvnI	CGCG	3 cut(s)	1374, 2147, 2338
NciI	CCSGG	2 cut(s)	818, 2364
NcoI	CCATGG	3 cut(s)	1047, 1442, 2452
NdeI	CATATG	1 cut(s)	758
NheI	GCTAGC	1 cut(s)	1655
NlaIV	GGNNCC	8 cut(s)	462, 587, 916, 940, 1102, 1600, 2167, 2383
NmeAIII	GCCGAG	1 cut(s)	1535
NmuCI	GTSAC	2 cut(s)	577, 1631
NruI	TCGCGA	2 cut(s)	2147, 2338
NspI	RCATGY	2 cut(s)	1349, 1609
NspV	TTCGAA	1 cut(s)	84
PagI	TCATGA	2 cut(s)	2076, 2181
PaqCI	CACCTGC	1 cut(s)	1151
PceI	AGGCCT	1 cut(s)	2561
PciI	ACATGT	1 cut(s)	1605
PcsI	WCGNNNNNNNCGW	1 cut(s)	2151
PctI	GAATGC	3 cut(s)	63, 867, 1161
PfeI	GAWTC	4 cut(s)	214, 566, 1123, 2448
PflFI	GACNNNGTC	1 cut(s)	1868
PinAI	ACCGGT	1 cut(s)	656
Ple19I	CGATCG	2 cut(s)	1653, 2336
PleI	GAGTC	3 cut(s)	1654, 2114, 2812
PpsI	GAGTC	3 cut(s)	1654, 2114, 2812
Ppu21I	YACGTR	2 cut(s)	2509, 2545
PscI	ACATGT	1 cut(s)	1605
Psp124BI	GAGCTC	1 cut(s)	2694
Psp6I	CCWGG	9 cut(s)	54, 463, 589, 846, 1103, 1112, 1178, 1882, 2561
PspFI	CCCAGC	2 cut(s)	1159, 1795
PspGI	CCWGG	9 cut(s)	54, 463, 589, 846, 1103, 1112, 1178, 1882, 2561
PspN4I	GGNNCC	8 cut(s)	462, 587, 916, 940, 1102, 1600, 2167, 2383
PspPI	GGNCC	2 cut(s)	152, 1198
PstI	CTGCAG	4 cut(s)	379, 508, 998, 2673
PsuI	RGATCY	4 cut(s)	144, 529, 2139, 2246
PsyI	GACNNNGTC	1 cut(s)	1868
PvuI	CGATCG	2 cut(s)	1653, 2336
PvuII	CAGCTG	1 cut(s)	2401
RruI	TCGCGA	2 cut(s)	2147, 2338
RsaI	GTAC	6 cut(s)	462, 587, 824, 1234, 2511, 2574
RsaNI	GTAC	6 cut(s)	461, 586, 823, 1233, 2510, 2573
RseI	CAYNNNNRTG	6 cut(s)	770, 1491, 1604, 1722, 1761, 2171
SacI	GAGCTC	1 cut(s)	2694
SalI	GTCGAC	1 cut(s)	50
SaqAI	TTAA	7 cut(s)	483, 1023, 1215, 1518, 2697, 2820, 2863
Sau96I	GGNCC	2 cut(s)	152, 1198
SchI	GAGTC	3 cut(s)	1655, 2114, 2813
SduI	GDGCHC	6 cut(s)	192, 1291, 1516, 1601, 2694, 2799
SfaNI	GCATC	7 cut(s)	499, 1155, 1279, 1780, 1825, 1834, 1868
SfcI	CTRYAG	6 cut(s)	375, 504, 994, 2491, 2669, 2713
SfuI	TTCGAA	1 cut(s)	84
SgrAI	CRCCGGYG	1 cut(s)	656
SinI	GGWCC	1 cut(s)	1198
SmiMI	CAYNNNNRTG	6 cut(s)	770, 1491, 1604, 1722, 1761, 2171
SmlI	CTYRAG	4 cut(s)	191, 2525, 2576, 2789
SmoI	CTYRAG	4 cut(s)	191, 2525, 2576, 2789
SnaBI	TACGTA	1 cut(s)	2509
SseBI	AGGCCT	1 cut(s)	2561
SspMI	CTAG	6 cut(s)	443, 1398, 1656, 1988, 2766, 2828
SstI	GAGCTC	1 cut(s)	2694
StuI	AGGCCT	1 cut(s)	2561
StyI	CCWWGG	8 cut(s)	261, 1047, 1170, 1442, 1481, 1627, 2161, 2452
TaaI	ACNGT	8 cut(s)	619, 889, 1036, 1408, 1493, 2188, 2666, 2860
TaiI	ACGT	4 cut(s)	1234, 2511, 2547, 2802
TatI	WGTACW	1 cut(s)	2572
TauI	GCSGC	5 cut(s)	383, 1377, 1584, 2207, 2556
TfiI	GAWTC	4 cut(s)	214, 566, 1123, 2448
Tru1I	TTAA	7 cut(s)	483, 1023, 1215, 1518, 2697, 2820, 2863
Tru9I	TTAA	7 cut(s)	483, 1023, 1215, 1518, 2697, 2820, 2863
TscAI	CASTG	8 cut(s)	565, 1003, 1227, 1498, 1552, 1833, 1909, 2863
TseFI	GTSAC	2 cut(s)	577, 1631
Tsp45I	GTSAC	2 cut(s)	577, 1631
TspDTI	ATGAA	7 cut(s)	138, 612, 653, 1740, 1955, 2026, 2521
TspGWI	ACGGA	5 cut(s)	540, 1967, 2082, 2243, 2628
TspRI	CASTG	8 cut(s)	565, 1003, 1227, 1498, 1552, 1833, 1909, 2863
Tth111I	GACNNNGTC	1 cut(s)	1868
VneI	GTGCAC	1 cut(s)	1287
VpaK11BI	GGWCC	1 cut(s)	1198
XapI	RAATTY	1 cut(s)	2234
XceI	RCATGY	2 cut(s)	1349, 1609
XcmI	CCANNNNNNNNNTGG	1 cut(s)	970
XmiI	GTMKAC	1 cut(s)	51
XspI	CTAG	6 cut(s)	443, 1398, 1656, 1988, 2766, 2828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.