Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009747

Physical Location & Seq

Genomic Coordinates Forward (+)

48164554 .. 48169358

Gene Structure 4805 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G029710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGGCAGAAGGCAAAGGGATTAGCTTGGAAGAAATCAAGAAGGAGAATGTTGACCTTGAGCGAATTCCAGTTCAGGAAGTGTTTGAGCAGTTGCAATGCACAAAAGAGGGGTTGTCAAGTGATGAAGGGCAGAAGAGGATTCAAGTCTTTGGCCCAAACAAGCTCGAGGAAAAAAAGGAAAACAAGATTCTGAAGTTCTTGGGTTTTATGTGGAATCCCCTATCATGGGTTATGGAAATTGCAGCTATCATGGCCATTGCCTTAGCAAATGGAGGGGGAAGGCCACCGGACTGGCAAGACTTTGTGGGAATTACTTCACTGTTATTCATCAACTCCACCATCAGCTTTATCGAAGAAAACAATGCAGGCAATGCTGCAGAAGCTCTTATGGCTGGTCTTGCCCCAAAAACCAAGGTTCTAAGAGATGGAAAATGGTCTGAGCTAGAGGCTGCAATCTTGGTACCGGGAGATGTAGTCAGCATCAAGTTGGGAGACATTGTCCCAGCTGATGCGCGTCTCTTGGAGGGAGATCCCCTCAAGATAGATCAGTCTGCCCTCACCGGTGAGTCTTTGCCTGTGACAAGGTTCCCCGGGGATGAAGTGTTCTCGGGTTCCACTGTCAAGCAAGGAGAAATTGAGGCAGTAGTCATTGCCACAGGGGTTCACACCTTCTTTGGTAAGGCTGCTCACCTGGTTGACAGCACCAACAATGTAGGCCACTTCCAAAAGGTTTTGACAGCTATTGGAAACTTCTGCATATGCTCTATTGCCATAGGAATGGTAATAGAACTAGTGGTTATGTATCCAATACAGCGTAGGAAGTACAGGGATGGGATTGACAATCTCTTGGTGCTTCTCATTGGAGGGATTCCGATTGCTATGCCAACAGTGTTGTCAGTTACCATGGCTATTGGTTCTCACCGCCTGTCCACACAAGGCGCTATCACAAAGAGGATGACAGCCATTGAGGAGATGGCTGGCATGGATGTCCTATGCAGTGATAAGACTGGGACCCTGACTCTCAACAAGCTTACAGTAGACAAGACCATGGTTGAGGTGTTTGTAAAGGATGTTGACAAGGATGGTCTCATTTTGCTCGGCGCCAGGGCTTCGAGGGTTGAGAATCAGGACGCTATTGATACATGTATTGTTGGAATGTTGGGCGACCCCAAGGAGGCCAGGGAAGGTATAACTGAGGTCCACTTTTTGCCCTTTAATCCAGTTGAAAAGCGTACAGCCATAACATACATTGATGCTGATGGCAATTGGCACCGAGTTAGCAAAGGTGCACCGGAGCAGATCATTGACCTCTGCGAGCTAAAAGACGATGCAAGGAAGAAAGCACATGCAATTATTGACAAGTTTGCAGATCGCGGTCTCCGTTCCCTTGCTGTTGCTAGACAGACTGTGCCAGAAAAAAACAAAGAGAGTGCTGGAACACCATGGCAGTTTGTAGGCCTCTTGCCTCTCTTTGATCCTCCCAGGCATGACAGTGCAGAGACCATTCGACGTGCCCTTCATCTTGGTGTTAATGTTAAGATGATCACTGGTGACCAACTTGCCATTGGTAAGGAAACTGGCCGCAGACTCGGCATGGGAACCAACATGTATCCTTCTTCTTCCCTCCTTGGTGAACACAAGGACGAGTCTGTTGCTTCCCTCCCTATTGATGAGCTTATTGAAAAGGCTGATGGCTTTGCTGGTGTCTTCCCAGAGCACAAATATGAAATTGTGAAGCGGCTGCAAGACAGGAAGCATATTTGTGGGATGACTGGAGATGGTGTGAATGATGCCCCAGCGCTAAAGAAGGCAGACATTGGTATTGCAGTTGATGATGCAACGGACGCTGCTCGGAGCGCCTCTGACATTGTCCTAACCGAACCAGGATTGTCTGTGATTATCAGTGCTGTGCTGACAAGCAGAGCCATTTTCCAGAGGATGAAGAACTACACCATCTATGCTGTTTCCATCACAATTCGTGTCGTCCTAGGATTTTTGCTCCTTGCTCTTATCTGGAAGTTTGACTTCTCACCTTTCATGGTTCTCATTATTGCCATACTTAATGATGGAACAATCATGACTATTTCAAAGGATAGGGTGAAGCCATCTCCTCTTCCAGACTCATGGAAGCTAAAGGAGATCTTTGCCACTGGTATCGTCCTTGGCACCTACATGGCTGTCATGACTGTTGTTTTCTTCTGGGCTGCTAATGATTCTGACTTTTTCTCAGACAAGTTTGGTGTAAGATCTATCAGAGGCAGCGATAAGGAGCTTACGGCAGCTGTCTACCTTCAAGTTAGTATTATCAGTCAGGCACTTATCTTTGTCACTCGATCCCGAAGCTGGTCTTTTGTAGAACGCCCCGGTGCCATGCTCATGATTGCCTTTTGCATAGCACAGCTGCTTGCTACAATTCTCGCTGTTTATGCGAATTGGGGCTTTGCAAGAATCAAAGGTATGGGATGGGGATGGGCAGGTGTTATCTGGCTCTACAGCATCATCACCTACATACCATTGGATATTCTCAAGTTCATAATTCGATATGCATTGAGTGGCAAGGCCTGGGATACTGTACTTGAGAATAAGACTGCCTTCACAACAAAGAAGGATTATGGAAAGGGAGAGAGGGAAGCACAATGGGCAACAGCTCAACGAACCCTGCATGGGCTGCAGCCTCCTGAAACATCTGAACTCTTCAATGACAAGAACAACTACCGAGAGCTGTCTGAGATCGCCGAACAGGCCAAGAGGCGAGCTGAAGTTGCTAGGTTGAGGGAGCTTCATACACTCAAGGGGCACGTGGAATCTGTTGTCAAACTCAAAGGACTTGATATCGACACCATTCAGCAACACTACACTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008553 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031090 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

954

Amino Acids

105.03

Weight (kDa)

6.14

Isoelectric Point (pI)

32.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	21 - 84	2.1e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	134 - 232	5.1e-30	P-type ATPase actuator domain
Hydrolase	PF00702	328 - 605	5.3e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	390 - 496	8.7e-06	P-type ATPase, cytoplasmic domain N

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009747)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80660 AT1G80660 AT1G80660 AT1G80660
fragaria_vesca	FvH4_6g28890
malus_domestica	MD09G1223600.v1.1
prunus_persica	Prupe.3G095900_v2.0.a1 Prupe.3G095900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0133471
rosa_laevigata	RLG00000019336
rosa_multiflora	Rmu_sc0004887.1_g000004 Rmu_sc0007236.1_g000003
rosa_rugosa	Rorug02G0311900 Rorug02G0311900 Rorug02G0311900 Rorug02G0312000
rosa_samantha	Rh2AG364500 Rh2BG370200 Rh2CG347800 Rh2DG387200
rosa_wichuraiana	Rw2G029710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2465
Acc36I	ACCTGC	1 cut(s)	2465
Acc65I	GGTACC	1 cut(s)	460
AccB1I	GGYRCC	5 cut(s)	460, 1100, 1269, 2165, 2366
AccI	GTMKAC	2 cut(s)	1038, 2286
AccII	CGCG	2 cut(s)	514, 1374
AciI	CCGC	4 cut(s)	922, 1374, 1582, 1738
AclWI	GGATC	3 cut(s)	524, 1469, 2328
AcoI	YGGCCR	2 cut(s)	252, 1579
AcsI	RAATTY	1 cut(s)	63
AcuI	CTGAAG	2 cut(s)	212, 2778
AcvI	CACGTG	1 cut(s)	2800
AcyI	GRCGYC	1 cut(s)	1101
AfaI	GTAC	4 cut(s)	462, 824, 1234, 2574
AfeI	AGCGCT	1 cut(s)	1800
AfiI	CCNNNNNNNGG	5 cut(s)	225, 226, 1174, 2343, 2664
AflIII	ACRYGT	2 cut(s)	1142, 1605
AgeI	ACCGGT	1 cut(s)	560
AgsI	TTSAA	6 cut(s)	143, 1226, 1682, 2088, 2294, 2698
AhlI	ACTAGT	1 cut(s)	790
AjiI	CACGTC	1 cut(s)	1511
AjnI	CCWGG	6 cut(s)	690, 1103, 1178, 1481, 1882, 2561
AloI	GAACNNNNNNTCC	2 cut(s)	587, 619
Alw21I	GWGCWC	2 cut(s)	1291, 1719
Alw26I	GTCTC	5 cut(s)	486, 521, 1091, 1382, 1493
Alw44I	GTGCAC	1 cut(s)	1287
AlwI	GGATC	3 cut(s)	524, 1469, 2328
Ama87I	CYCGRG	3 cut(s)	164, 590, 607
Aor51HI	AGCGCT	1 cut(s)	1800
AoxI	GGCC	9 cut(s)	151, 252, 281, 715, 1176, 1456, 1579, 2559, 2742
ApaLI	GTGCAC	1 cut(s)	1287
ApoI	RAATTY	1 cut(s)	63
AsiGI	ACCGGT	1 cut(s)	560
Asp700I	GAANNNNTTC	1 cut(s)	313
Asp718I	GGTACC	1 cut(s)	460
AspA2I	CCTAGG	1 cut(s)	1987
AspLEI	GCGC	5 cut(s)	514, 941, 1103, 1801, 1859
AspS9I	GGNCC	3 cut(s)	152, 1011, 1198
AsuC2I	CCSGG	4 cut(s)	465, 591, 592, 2364
AsuHPI	GGTGA	9 cut(s)	550, 575, 680, 911, 1562, 1643, 2022, 2110, 2494
AvaI	CYCGRG	3 cut(s)	164, 590, 607
AvaII	GGWCC	2 cut(s)	1011, 1198
AvrII	CCTAGG	1 cut(s)	1987
BaeGI	GKGCMC	3 cut(s)	1291, 1516, 2799
BalI	TGGCCA	1 cut(s)	254
BanI	GGYRCC	5 cut(s)	460, 1100, 1269, 2165, 2366
BbrPI	CACGTG	1 cut(s)	2800
BbsI	GAAGAC	1 cut(s)	1699
Bbv12I	GWGCWC	2 cut(s)	1291, 1719
BceAI	ACGGC	1 cut(s)	2292
BcgI	CGANNNNNNTGC	4 cut(s)	862, 896, 1634, 1668
BciT130I	CCWGG	6 cut(s)	692, 1105, 1180, 1483, 1884, 2563
BciVI	GTATCC	3 cut(s)	813, 1620, 2560
BclI	TGATCA	1 cut(s)	1542
BcnI	CCSGG	4 cut(s)	465, 591, 592, 2364
BcoDI	GTCTC	5 cut(s)	486, 521, 1091, 1382, 1493
BcuI	ACTAGT	1 cut(s)	790
BfaI	CTAG	5 cut(s)	443, 791, 1398, 1988, 2766
BfmI	CTRYAG	3 cut(s)	375, 2491, 2669
BfoI	RGCGCY	4 cut(s)	942, 1104, 1802, 1860
BfuAI	ACCTGC	1 cut(s)	2465
BfuI	GTATCC	3 cut(s)	813, 1620, 2560
BglI	GCCNNNNNGGC	1 cut(s)	2741
BglII	AGATCT	2 cut(s)	2139, 2246
BlnI	CCTAGG	1 cut(s)	1987
Bme18I	GGWCC	2 cut(s)	1011, 1198
BmeT110I	CYCGRG	3 cut(s)	164, 590, 607
BmgBI	CACGTC	1 cut(s)	1511
BmgT120I	GGNCC	3 cut(s)	152, 1011, 1198
BmrI	ACTGGG	1 cut(s)	1017
BmsI	GCATC	7 cut(s)	489, 499, 1243, 1318, 1780, 1825, 2505
BmuI	ACTGGG	1 cut(s)	1017
BpiI	GAAGAC	1 cut(s)	1699
BplI	GAGNNNNNCTC	2 cut(s)	519, 551
BpmI	CTGGAG	1 cut(s)	1794
Bpu10I	CCTNAGC	1 cut(s)	262
BpuEI	CTTGAG	5 cut(s)	77, 521, 2510, 2597, 2774
BpuMI	CCSGG	4 cut(s)	465, 591, 592, 2364
BsaAI	YACGTR	1 cut(s)	2800
BsaHI	GRCGYC	1 cut(s)	1101
BsaI	GGTCTC	3 cut(s)	1091, 1382, 1493
BsaWI	WCCGGW	3 cut(s)	286, 560, 1291
BsaXI	ACNNNNNCTCC	6 cut(s)	459, 483, 489, 513, 1767, 1797
Bsc4I	CCNNNNNNNGG	5 cut(s)	225, 226, 1174, 2343, 2664
Bse118I	RCCGGY	1 cut(s)	560
Bse1I	ACTGG	8 cut(s)	68, 296, 1012, 1220, 1552, 1582, 1777, 2155
Bse3DI	GCAATG	4 cut(s)	101, 255, 376, 648
BseBI	CCWGG	6 cut(s)	692, 1105, 1180, 1483, 1884, 2563
BseLI	CCNNNNNNNGG	5 cut(s)	225, 226, 1174, 2343, 2664
BseMI	GCAATG	4 cut(s)	101, 255, 376, 648
BseMII	CTCAG	4 cut(s)	429, 1185, 2241, 2718
BseNI	ACTGG	8 cut(s)	68, 296, 1012, 1220, 1552, 1582, 1777, 2155
BseRI	GAGGAG	2 cut(s)	983, 2100
BseSI	GKGCMC	3 cut(s)	1291, 1516, 2799
BseYI	CCCAGC	2 cut(s)	502, 1795
BsgI	GTGCAG	1 cut(s)	1515
Bsh1236I	CGCG	2 cut(s)	514, 1374
BshFI	GGCC	9 cut(s)	153, 254, 283, 717, 1178, 1458, 1581, 2561, 2744
BshNI	GGYRCC	5 cut(s)	460, 1100, 1269, 2165, 2366
BshTI	ACCGGT	1 cut(s)	560
BsiHKAI	GWGCWC	2 cut(s)	1291, 1719
BsiHKCI	CYCGRG	3 cut(s)	164, 590, 607
BsiSI	CCGG	6 cut(s)	287, 464, 561, 591, 1292, 2364
BslFI	GGGAC	2 cut(s)	485, 1024
BslI	CCNNNNNNNGG	5 cut(s)	225, 226, 1174, 2343, 2664
BsmAI	GTCTC	5 cut(s)	486, 521, 1091, 1382, 1493
BsmBI	CGTCTC	1 cut(s)	521
BsmFI	GGGAC	2 cut(s)	485, 1024
BsnI	GGCC	9 cut(s)	153, 254, 283, 717, 1178, 1458, 1581, 2561, 2744
Bso31I	GGTCTC	3 cut(s)	1091, 1382, 1493
BsoBI	CYCGRG	3 cut(s)	164, 590, 607
Bsp1286I	GDGCHC	4 cut(s)	1291, 1516, 1719, 2799
Bsp19I	CCATGG	3 cut(s)	903, 1047, 1442
BspACI	CCGC	4 cut(s)	922, 1374, 1582, 1738
BspANI	GGCC	9 cut(s)	153, 254, 283, 717, 1178, 1458, 1581, 2561, 2744
BspCNI	CTCAG	4 cut(s)	430, 1186, 2240, 2719
BspFNI	CGCG	2 cut(s)	514, 1374
BspHI	TCATGA	3 cut(s)	2076, 2181, 2376
BspMAI	CTGCAG	2 cut(s)	379, 2673
BspMI	ACCTGC	1 cut(s)	2465
BspPI	GGATC	3 cut(s)	524, 1469, 2328
BspT107I	GGYRCC	5 cut(s)	460, 1100, 1269, 2165, 2366
BspTNI	GGTCTC	3 cut(s)	1091, 1382, 1493
BsrDI	GCAATG	4 cut(s)	101, 255, 376, 648
BsrFI	RCCGGY	1 cut(s)	560
BsrI	ACTGG	8 cut(s)	68, 296, 1012, 1220, 1552, 1582, 1777, 2155
BssAI	RCCGGY	1 cut(s)	560
BssNI	GRCGYC	1 cut(s)	1101
BssT1I	CCWWGG	8 cut(s)	411, 903, 1047, 1170, 1442, 1627, 1987, 2161
Bst2UI	CCWGG	6 cut(s)	692, 1105, 1180, 1483, 1884, 2563
Bst4CI	ACNGT	9 cut(s)	321, 619, 889, 1036, 1408, 1493, 2188, 2572, 2860
Bst6I	CTCTTC	3 cut(s)	128, 2118, 2699
BstACI	GRCGYC	1 cut(s)	1101
BstAPI	GCANNNNNTGC	2 cut(s)	371, 2668
BstBAI	YACGTR	1 cut(s)	2800
BstC8I	GCNNGC	5 cut(s)	367, 979, 1316, 2406, 2754
BstDEI	CTNAG	6 cut(s)	262, 419, 438, 1194, 2227, 2727
BstDSI	CCRYGG	3 cut(s)	903, 1047, 1442
BstEII	GGTNACC	1 cut(s)	1550
BstFNI	CGCG	2 cut(s)	514, 1374
BstH2I	RGCGCY	4 cut(s)	942, 1104, 1802, 1860
BstHHI	GCGC	5 cut(s)	514, 941, 1103, 1801, 1859
BstMAI	GTCTC	5 cut(s)	486, 521, 1091, 1382, 1493
BstNI	CCWGG	6 cut(s)	692, 1105, 1180, 1483, 1884, 2563
BstNSI	RCATGY	3 cut(s)	1146, 1349, 1609
BstPI	GGTNACC	1 cut(s)	1550
BstSFI	CTRYAG	3 cut(s)	375, 2491, 2669
BstSLI	GKGCMC	3 cut(s)	1291, 1516, 2799
BstUI	CGCG	2 cut(s)	514, 1374
BstV2I	GAAGAC	1 cut(s)	1699
BstX2I	RGATCY	3 cut(s)	529, 2139, 2246
BstXI	CCANNNNNNTGG	3 cut(s)	291, 778, 2124
BstYI	RGATCY	3 cut(s)	529, 2139, 2246
BsuI	GTATCC	3 cut(s)	813, 1620, 2560
BsuRI	GGCC	9 cut(s)	153, 254, 283, 717, 1178, 1458, 1581, 2561, 2744
BtgI	CCRYGG	3 cut(s)	903, 1047, 1442
BtrI	CACGTC	1 cut(s)	1511
BtsI	GCAGTG	1 cut(s)	1003
BtsIMutI	CAGTG	9 cut(s)	317, 615, 894, 1003, 1498, 1545, 1909, 2148, 2856
BveI	ACCTGC	1 cut(s)	2465
Cac8I	GCNNGC	5 cut(s)	367, 979, 1316, 2406, 2754
CciI	TCATGA	3 cut(s)	2076, 2181, 2376
CfoI	GCGC	5 cut(s)	514, 941, 1103, 1801, 1859
Cfr10I	RCCGGY	1 cut(s)	560
Cfr13I	GGNCC	3 cut(s)	152, 1011, 1198
Cfr9I	CCCGGG	1 cut(s)	590
CseI	GACGC	3 cut(s)	503, 1139, 1853
CsiI	ACCWGGT	1 cut(s)	690
Csp6I	GTAC	4 cut(s)	461, 823, 1233, 2573
CspAI	ACCGGT	1 cut(s)	560
CviQI	GTAC	4 cut(s)	461, 823, 1233, 2573
DdeI	CTNAG	6 cut(s)	262, 419, 438, 1194, 2227, 2727
DinI	GGCGCC	1 cut(s)	1102
EaeI	YGGCCR	2 cut(s)	252, 1579
Eam1104I	CTCTTC	3 cut(s)	128, 2118, 2699
EarI	CTCTTC	3 cut(s)	128, 2118, 2699
Eco130I	CCWWGG	8 cut(s)	411, 903, 1047, 1170, 1442, 1627, 1987, 2161
Eco147I	AGGCCT	2 cut(s)	1458, 2561
Eco31I	GGTCTC	3 cut(s)	1091, 1382, 1493
Eco32I	GATATC	1 cut(s)	2833
Eco47I	GGWCC	2 cut(s)	1011, 1198
Eco47III	AGCGCT	1 cut(s)	1800
Eco57I	CTGAAG	2 cut(s)	212, 2778
Eco72I	CACGTG	1 cut(s)	2800
Eco88I	CYCGRG	3 cut(s)	164, 590, 607
Eco91I	GGTNACC	1 cut(s)	1550
EcoO109I	RGGNCCY	1 cut(s)	1011
EcoO65I	GGTNACC	1 cut(s)	1550
EcoRI	GAATTC	1 cut(s)	63
EcoRII	CCWGG	6 cut(s)	690, 1103, 1178, 1481, 1882, 2561
EcoRV	GATATC	1 cut(s)	2833
EcoT14I	CCWWGG	8 cut(s)	411, 903, 1047, 1170, 1442, 1627, 1987, 2161
EcoT22I	ATGCAT	1 cut(s)	2548
EgeI	GGCGCC	1 cut(s)	1102
EheI	GGCGCC	1 cut(s)	1102
ErhI	CCWWGG	8 cut(s)	411, 903, 1047, 1170, 1442, 1627, 1987, 2161
Esp3I	CGTCTC	1 cut(s)	521
FalI	AAGNNNNNCTT	8 cut(s)	2009, 2041, 2126, 2158, 2332, 2364, 2576, 2608
FaqI	GGGAC	2 cut(s)	485, 1024
FauNDI	CATATG	1 cut(s)	758
FbaI	TGATCA	1 cut(s)	1542
FblI	GTMKAC	2 cut(s)	1038, 2286
FspBI	CTAG	5 cut(s)	443, 791, 1398, 1988, 2766
GlaI	GCGC	5 cut(s)	513, 940, 1102, 1800, 1858
GsaI	CCCAGC	2 cut(s)	506, 1799
GsuI	CTGGAG	1 cut(s)	1794
HaeII	RGCGCY	4 cut(s)	942, 1104, 1802, 1860
HaeIII	GGCC	9 cut(s)	153, 254, 283, 717, 1178, 1458, 1581, 2561, 2744
HapII	CCGG	6 cut(s)	287, 464, 561, 591, 1292, 2364
HgaI	GACGC	3 cut(s)	503, 1139, 1853
HhaI	GCGC	5 cut(s)	514, 941, 1103, 1801, 1859
Hin1I	GRCGYC	1 cut(s)	1101
Hin6I	GCGC	5 cut(s)	512, 939, 1101, 1799, 1857
HinP1I	GCGC	5 cut(s)	512, 939, 1101, 1799, 1857
HincII	GTYRAC	3 cut(s)	52, 697, 1075
HindII	GTYRAC	3 cut(s)	52, 697, 1075
HindIII	AAGCTT	1 cut(s)	1028
HpaII	CCGG	6 cut(s)	287, 464, 561, 591, 1292, 2364
HphI	GGTGA	9 cut(s)	550, 575, 680, 911, 1562, 1643, 2022, 2110, 2494
Hpy99I	CGWCG	1 cut(s)	1512
HpyCH4III	ACNGT	9 cut(s)	321, 619, 889, 1036, 1408, 1493, 2188, 2572, 2860
HpyCH4IV	ACGT	2 cut(s)	1510, 2799
HpyF3I	CTNAG	6 cut(s)	262, 419, 438, 1194, 2227, 2727
HpySE526I	ACGT	2 cut(s)	1510, 2799
Hsp92I	GRCGYC	1 cut(s)	1101
HspAI	GCGC	5 cut(s)	512, 939, 1101, 1799, 1857
KasI	GGCGCC	1 cut(s)	1100
KflI	GGGWCCC	1 cut(s)	1011
KpnI	GGTACC	1 cut(s)	464
Ksp22I	TGATCA	1 cut(s)	1542
LmnI	GCTCC	5 cut(s)	1294, 1854, 2004, 2269, 2776
LweI	GCATC	7 cut(s)	489, 499, 1243, 1318, 1780, 1825, 2505
MabI	ACCWGGT	1 cut(s)	690
MaeI	CTAG	5 cut(s)	443, 791, 1398, 1988, 2766
MaeII	ACGT	2 cut(s)	1510, 2799
MaeIII	GTNAC	4 cut(s)	577, 898, 1550, 2326
MfeI	CAATTG	1 cut(s)	1264
MflI	RGATCY	3 cut(s)	529, 2139, 2246
MhlI	GDGCHC	4 cut(s)	1291, 1516, 1719, 2799
MlsI	TGGCCA	1 cut(s)	254
MluNI	TGGCCA	1 cut(s)	254
Mly113I	GGCGCC	1 cut(s)	1101
MlyI	GAGTC	5 cut(s)	575, 1012, 1581, 1655, 2114
MmeI	TCCRAC	1 cut(s)	1132
Mox20I	TGGCCA	1 cut(s)	254
Mph1103I	ATGCAT	1 cut(s)	2548
MroXI	GAANNNNTTC	1 cut(s)	313
MscI	TGGCCA	1 cut(s)	254
MseI	TTAA	4 cut(s)	1215, 1530, 1536, 2061
MslI	CAYNNNNRTG	7 cut(s)	776, 1491, 1524, 1722, 1761, 2171, 2375
Msp20I	TGGCCA	1 cut(s)	254
MspA1I	CMGCKG	3 cut(s)	506, 2282, 2401
MspI	CCGG	6 cut(s)	287, 464, 561, 591, 1292, 2364
MunI	CAATTG	1 cut(s)	1264
MvaI	CCWGG	6 cut(s)	692, 1105, 1180, 1483, 1884, 2563
MvnI	CGCG	2 cut(s)	514, 1374
NarI	GGCGCC	1 cut(s)	1101
NciI	CCSGG	4 cut(s)	465, 591, 592, 2364
NcoI	CCATGG	3 cut(s)	903, 1047, 1442
NdeI	CATATG	1 cut(s)	758
NmeAIII	GCCGAG	2 cut(s)	1077, 1569
NmuCI	GTSAC	3 cut(s)	577, 1550, 2326
NsiI	ATGCAT	1 cut(s)	2548
NspI	RCATGY	3 cut(s)	1146, 1349, 1609
PaeR7I	CTCGAG	1 cut(s)	164
PagI	TCATGA	3 cut(s)	2076, 2181, 2376
PaqCI	CACCTGC	1 cut(s)	2465
PceI	AGGCCT	2 cut(s)	1458, 2561
PciI	ACATGT	2 cut(s)	1142, 1605
PcsI	WCGNNNNNNNCGW	1 cut(s)	1378
PdmI	GAANNNNTTC	1 cut(s)	313
PfeI	GAWTC	8 cut(s)	139, 187, 214, 868, 1123, 2213, 2448, 2804
PflFI	GACNNNGTC	2 cut(s)	497, 1868
PinAI	ACCGGT	1 cut(s)	560
PleI	GAGTC	5 cut(s)	574, 1012, 1581, 1654, 2114
PluTI	GGCGCC	1 cut(s)	1104
PmaCI	CACGTG	1 cut(s)	2800
PmlI	CACGTG	1 cut(s)	2800
PpsI	GAGTC	5 cut(s)	574, 1012, 1581, 1654, 2114
Ppu21I	YACGTR	1 cut(s)	2800
PpuMI	RGGWCCY	1 cut(s)	1011
PscI	ACATGT	2 cut(s)	1142, 1605
Psp5II	RGGWCCY	1 cut(s)	1011
Psp6I	CCWGG	6 cut(s)	690, 1103, 1178, 1481, 1882, 2561
PspCI	CACGTG	1 cut(s)	2800
PspEI	GGTNACC	1 cut(s)	1550
PspFI	CCCAGC	2 cut(s)	502, 1795
PspGI	CCWGG	6 cut(s)	690, 1103, 1178, 1481, 1882, 2561
PspPI	GGNCC	3 cut(s)	152, 1011, 1198
PspPPI	RGGWCCY	1 cut(s)	1011
PspXI	VCTCGAGB	1 cut(s)	164
PstI	CTGCAG	2 cut(s)	379, 2673
PsuI	RGATCY	3 cut(s)	529, 2139, 2246
PsyI	GACNNNGTC	2 cut(s)	497, 1868
PvuII	CAGCTG	3 cut(s)	506, 2282, 2401
RsaI	GTAC	4 cut(s)	462, 824, 1234, 2574
RsaNI	GTAC	4 cut(s)	461, 823, 1233, 2573
RseI	CAYNNNNRTG	7 cut(s)	776, 1491, 1524, 1722, 1761, 2171, 2375
SaqAI	TTAA	4 cut(s)	1215, 1530, 1536, 2061
Sau96I	GGNCC	3 cut(s)	152, 1011, 1198
SchI	GAGTC	5 cut(s)	575, 1012, 1581, 1655, 2114
SduI	GDGCHC	4 cut(s)	1291, 1516, 1719, 2799
SexAI	ACCWGGT	1 cut(s)	690
SfaNI	GCATC	7 cut(s)	489, 499, 1243, 1318, 1780, 1825, 2505
SfcI	CTRYAG	3 cut(s)	375, 2491, 2669
SfoI	GGCGCC	1 cut(s)	1102
Sfr274I	CTCGAG	1 cut(s)	164
SgrAI	CRCCGGYG	1 cut(s)	560
SinI	GGWCC	2 cut(s)	1011, 1198
SlaI	CTCGAG	1 cut(s)	164
SmaI	CCCGGG	1 cut(s)	592
SmiMI	CAYNNNNRTG	7 cut(s)	776, 1491, 1524, 1722, 1761, 2171, 2375
SmlI	CTYRAG	6 cut(s)	56, 164, 536, 2525, 2576, 2789
SmoI	CTYRAG	6 cut(s)	56, 164, 536, 2525, 2576, 2789
SpeI	ACTAGT	1 cut(s)	790
SseBI	AGGCCT	2 cut(s)	1458, 2561
SsiI	CCGC	4 cut(s)	922, 1374, 1582, 1738
SspDI	GGCGCC	1 cut(s)	1100
SspMI	CTAG	5 cut(s)	443, 791, 1398, 1988, 2766
StuI	AGGCCT	2 cut(s)	1458, 2561
StyI	CCWWGG	8 cut(s)	411, 903, 1047, 1170, 1442, 1627, 1987, 2161
TaaI	ACNGT	9 cut(s)	321, 619, 889, 1036, 1408, 1493, 2188, 2572, 2860
TaiI	ACGT	2 cut(s)	1513, 2802
TaqI	TCGA	7 cut(s)	165, 351, 1112, 1507, 2332, 2539, 2835
TatI	WGTACW	2 cut(s)	822, 2572
TauI	GCSGC	2 cut(s)	1584, 1741
TfiI	GAWTC	8 cut(s)	139, 187, 214, 868, 1123, 2213, 2448, 2804
Tru1I	TTAA	4 cut(s)	1215, 1530, 1536, 2061
Tru9I	TTAA	4 cut(s)	1215, 1530, 1536, 2061
TscAI	CASTG	9 cut(s)	324, 622, 894, 1003, 1498, 1552, 1909, 2155, 2863
TseFI	GTSAC	3 cut(s)	577, 1550, 2326
Tsp45I	GTSAC	3 cut(s)	577, 1550, 2326
TspDTI	ATGAA	9 cut(s)	138, 316, 612, 1508, 1740, 1955, 2026, 2521, 2771
TspGWI	ACGGA	2 cut(s)	1370, 1856
TspMI	CCCGGG	1 cut(s)	590
TspRI	CASTG	9 cut(s)	324, 622, 894, 1003, 1498, 1552, 1909, 2155, 2863
Tth111I	GACNNNGTC	2 cut(s)	497, 1868
VneI	GTGCAC	1 cut(s)	1287
VpaK11BI	GGWCC	2 cut(s)	1011, 1198
XapI	RAATTY	1 cut(s)	63
XceI	RCATGY	3 cut(s)	1146, 1349, 1609
XcmI	CCANNNNNNNNNTGG	2 cut(s)	970, 1562
XhoI	CTCGAG	1 cut(s)	164
XmaI	CCCGGG	1 cut(s)	590
XmaJI	CCTAGG	1 cut(s)	1987
XmiI	GTMKAC	2 cut(s)	1038, 2286
XmnI	GAANNNNTTC	1 cut(s)	313
XspI	CTAG	5 cut(s)	443, 791, 1398, 1988, 2766
Zsp2I	ATGCAT	1 cut(s)	2548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.