MD09G1236700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004364
Physical Location & Seq
Forward (+)
29895392 .. 29913230
17839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1236700.v1.1.491

Sequence Viewer

Length: 2364 bp
ATGAAGGAGACACGAGATGATACAGGGCCAGCAGAGCAAAAGCCTCCAAATGCTTCATGGTGGCCTTCAGATTTCGTGGACAAATTTGGATCTGTTTCTTTGGGATCCCAAGAAGAATCATTGAGTAGTACTGAATCACCTAGAAGTTCGGATCAAGATGTGTTGTCGTCCCAGAAGGCATCACAAGTTGTATGGCAAACTGGAATGCTTTTAGAACCGATTCCCAATGGTTTCTATTTTGTCATTCCGGAGAAAAGGCTCAAGGAGATCTTTGATGATATTCCTACTCTTGATGAGCTTCATGCTCTTGAGGCAGAGGGTTTGAGAGCCAATGTCATTCTTGTAGATACTGCAAAGGACAAAAAGCTGTCCATGCTGAAGCAACTTATTGTGGCTCTGGTGAAAGGATTGAACTCATATCCAGCTGAAATGATAAAAAAGATTGCTGGATTGGTTTCCGATTTTTATAAGAGGCCAAATGCAGAAAGTCCAGCAAAAGCTGCACTTGAAGAAACCTCCCACATGTTTGAGAATCGAGGAGTTCAACTTTTGGGGCAAATAAAACATGGTTCATGCCGTCCTAGAGCAATCTTATTCAAAGTTCTGGCGGACACCGTAGGTCTTGAAAGTAGGCTAATGGTGGGATTGCCTATTGATGGAGTCGTTGAGTCTGCAGATTCATATAAGCATATGTCGGTGGTAGTTGTATTGAACTCTGTTGAATTATTGGTTGACCTGATGCGGTTTCCTGGTAAGCTGTTACCCAGATCAACCAAGGCAATTTTTTTGACCCATATTTCTGCAGCCGGGGAGAGTGATAGTGCAGAGAATGACTCGTGCGATTCACCACTAGAACCGAACAGTCCTCTATATGGATTCTCAGAGAGAGTGGATCCTGACAGTGCTGAAAAAGATGATAGCCCTCAGTTCCGAGGTAGATTTGATGTGCCCTCGAATGCACAAGGTCCCTCATTACAAAATATGATGTTGCGATCAATTACTTCCATGGAAAGAAAACTGAGTTTATCACATAGTGAACCCAACATTGCAACTACATTTTGTCGGCGTAGTCGGAGGACGGCCATTGCTGAACAGCGGACTGCTAGTTCAAGTCCAGAGCATCCTTCATTCCGAGCACGTGGGCGGTCCATGCTCAGTGGTGATAGGAAATCTTTTAGAGATTATGCTGATGACATGCCTACCTCAAGCTACAGGTCAGATGGTGCATCAACATCAGAAGCTCGCAGATTAAGAAGAAGAAGCATGAGTATTACTCCAGAGATTGGTGATGATATCGTAAGGGCTGTGCGAGCAATGAATGAAACATTGAAGCAGAATCGACTTTTGAGAGAGCAGGGTGAGGATGACAGATCTGCTCTTCAGAAAAATGACCAAATCAGTTCTCAAAAAGCAATGTCATTACCTTCATCTCCACATCAGTACAGAAGTCAAACTTCTGATAGAATTGGACCTTCAGAGTTCACGAGATATGATGAATTGGTCCTAACATGGAACAGAATTCTGGAGTCCGCCACATTTGATAATAAGCCTCTATTGCCTTACCAGGAATGGAATATTGATTTCTCAGAATTGACTGTTGGAACTCGTGTTGGAATAGGGTTCTTTGGAGAAGTCTTTCGAGGCGTTTGGAATGGAACAGATGTCGCAATAAAGGTTTTCTTAGAGCAAGACCTAACTGCTGAAAACATGGAAGACTTCTGCAATGAGATATCCATTCTTAGTCGCCTTCGGCATCCTAATGTTATTCTATTTCTGGGTGCATGCACAAAGCCTCCACGCTTATCAATGGTTACAGAATACATGGAGATGGGATCTTTGTATTATTTGATTCATTTGAGTGGCCAGAAGAAGAAGCTTAGCTGGCGAAGGAGACTAAAAATGTTGCGTGACATATGCAGGGGGTTGATGTGCATGCACCGGATGAAGATAGTTCATCGTGATCTAAAAAGTGCAAATTGTCTCGTGAATAAGCACTGGACTGTCAAGATCTGTGACTTTGGACTCTCGAGAATAATGTCCGAGACAAACATGAAGGACTCATCGTCTGCAGGAACTCCGGAATGGATGGCACCTGAGCTTATACGAAATGAACCCTTCACTGAAAAATGTGATATTTTCAGCCTTGGGGTTTTAATGTGGGAGCTCTGCACTCTAAATAGACCGTGGGAAGGCGTTCCACCAGAGAGGGTAGTTTACGCTGTCGCGAATGAAGGTTCCCGGTTGGAGATCCCAGAAGGTCCCCTAGGCAGGCTTATTTCAGATTGTTGGACGGAACCGCAGGATCGACCGAGCTGCGAGGAGATACTCTCCCGCTTGTTAGGCTGCGAGTACTCACTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 ko04013 ko04064 ko04140 ko04152 ko04214 ko04310 ko04380 ko04520 ko04620 ko04621 ko04622 ko04624 ko04657 ko04660 ko04668 ko05140 ko05145 ko05162 ko05168 ko05169 ko05418 map04010 map04013 map04064 map04140 map04152 map04214 map04310 map04380 map04520 map04620 map04621 map04622 map04624 map04657 map04660 map04668 map05140 map05145 map05162 map05168 map05169 map05418
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

788

Amino Acids

88.68

Weight (kDa)

6.03

Isoelectric Point (pI)

58.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 57 - 253 1.2e-51 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 532 - 779 6.5e-63 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 533 - 777 3.9e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 2089
AccB7I CCANNNNNTGG 1 cut(s) 1283
AccII CGCG 1 cut(s) 2225
AccIII TCCGGA 2 cut(s) 247, 2077
AciI CCGC 7 cut(s) 608, 742, 1096, 1144, 1530, 2297, 2332
AclWI GGATC 9 cut(s) 97, 99, 112, 159, 887, 900, 1840, 2242, 2310
AcoI YGGCCR 2 cut(s) 1080, 1861
AcsI RAATTY 2 cut(s) 83, 1518
AcuI CTGAAG 4 cut(s) 51, 398, 1364, 1458
AcvI CACGTG 1 cut(s) 1139
AdeI CACNNNGTG 1 cut(s) 1034
AfaI GTAC 3 cut(s) 130, 1442, 2351
AfiI CCNNNNNNNGG 5 cut(s) 656, 872, 1283, 2189, 2268
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 9 cut(s) 412, 509, 545, 598, 626, 712, 722, 1110, 1330
AhdI GACNNNNNGTC 1 cut(s) 2062
AjnI CCWGG 2 cut(s) 748, 1563
AjuI GAANNNNNNNTTGG 2 cut(s) 1581, 1613
AloI GAACNNNNNNTCC 2 cut(s) 1090, 1122
Alw21I GWGCWC 2 cut(s) 1138, 2166
Alw26I GTCTC 4 cut(s) 2, 1885, 1985, 2036
AlwI GGATC 9 cut(s) 97, 99, 112, 159, 887, 900, 1840, 2242, 2310
Ama87I CYCGRG 1 cut(s) 2026
Aor13HI TCCGGA 2 cut(s) 247, 2077
AoxI GGCC 5 cut(s) 26, 62, 473, 1080, 1861
ApeKI GCWGC 4 cut(s) 500, 803, 2313, 2343
ApoI RAATTY 2 cut(s) 83, 1518
Asp700I GAANNNNTTC 3 cut(s) 219, 1635, 2193
AspA2I CCTAGG 1 cut(s) 2263
AspS9I GGNCC 6 cut(s) 26, 965, 1146, 1469, 1501, 2258
AsuC2I CCSGG 2 cut(s) 808, 2239
AsuHPI GGTGA 6 cut(s) 129, 412, 837, 1172, 1298, 1370
AvaI CYCGRG 1 cut(s) 2026
AvaII GGWCC 5 cut(s) 965, 1146, 1469, 1501, 2258
AvrII CCTAGG 1 cut(s) 2263
BaeGI GKGCMC 1 cut(s) 951
BalI TGGCCA 1 cut(s) 1863
BamHI GGATCC 2 cut(s) 104, 892
BanI GGYRCC 1 cut(s) 2089
BanII GRGCYC 1 cut(s) 2166
BauI CACGAG 5 cut(s) 12, 835, 1483, 1605, 1982
BbrPI CACGTG 1 cut(s) 1139
BbsI GAAGAC 1 cut(s) 1719
Bbv12I GWGCWC 2 cut(s) 1138, 2166
BbvI GCAGC 4 cut(s) 487, 815, 2300, 2330
BccI CCATC 4 cut(s) 650, 1214, 1822, 2080
BceAI ACGGC 2 cut(s) 561, 1095
BcgI CGANNNNNNTGC 2 cut(s) 2295, 2329
BciT130I CCWGG 2 cut(s) 750, 1565
BcnI CCSGG 2 cut(s) 808, 2239
BcoDI GTCTC 4 cut(s) 2, 1885, 1985, 2036
BfaI CTAG 5 cut(s) 141, 582, 851, 1104, 2264
BfmI CTRYAG 4 cut(s) 672, 801, 1210, 2067
BglII AGATCT 3 cut(s) 267, 1370, 2007
BisI GCNGC 4 cut(s) 501, 804, 2314, 2344
BlnI CCTAGG 1 cut(s) 2263
BlpI GCTNAGC 1 cut(s) 1877
BlsI GCNGC 4 cut(s) 502, 805, 2315, 2345
BmcAI AGTACT 2 cut(s) 130, 2351
Bme1390I CCNGG 4 cut(s) 750, 808, 1565, 2239
Bme18I GGWCC 5 cut(s) 965, 1146, 1469, 1501, 2258
BmeRI GACNNNNNGTC 1 cut(s) 2062
BmeT110I CYCGRG 1 cut(s) 2026
BmgT120I GGNCC 6 cut(s) 26, 965, 1146, 1469, 1501, 2258
BmiI GGNNCC 7 cut(s) 106, 894, 967, 2091, 2236, 2260, 2295
BmrFI CCNGG 4 cut(s) 750, 808, 1565, 2239
BmsI GCATC 5 cut(s) 188, 729, 1129, 1235, 1762
BpiI GAAGAC 1 cut(s) 1719
BplI GAGNNNNNCTC 6 cut(s) 818, 850, 1258, 1290, 2312, 2344
BpmI CTGGAG 2 cut(s) 1260, 1544
Bpu10I CCTNAGC 1 cut(s) 2094
Bpu1102I GCTNAGC 1 cut(s) 1877
BpuEI CTTGAG 3 cut(s) 245, 329, 1189
BpuMI CCSGG 2 cut(s) 808, 2239
BsaAI YACGTR 1 cut(s) 1139
BsaJI CCNNGG 7 cut(s) 774, 807, 931, 1005, 2143, 2183, 2263
BsaWI WCCGGW 3 cut(s) 247, 1938, 2077
BsaXI ACNNNNNCTCC 2 cut(s) 1777, 1807
Bsc4I CCNNNNNNNGG 5 cut(s) 656, 872, 1283, 2189, 2268
Bse1I ACTGG 2 cut(s) 205, 2000
Bse3DI GCAATG 5 cut(s) 1044, 1083, 1320, 1419, 1729
BseAI TCCGGA 2 cut(s) 247, 2077
BseBI CCWGG 2 cut(s) 750, 1565
BseDI CCNNGG 7 cut(s) 774, 807, 931, 1005, 2143, 2183, 2263
BseGI GGATG 5 cut(s) 1120, 1369, 1753, 1947, 2091
BseLI CCNNNNNNNGG 5 cut(s) 656, 872, 1283, 2189, 2268
BseMI GCAATG 5 cut(s) 1044, 1083, 1320, 1419, 1729
BseMII CTCAG 6 cut(s) 894, 938, 1010, 1168, 1599, 2085
BseNI ACTGG 2 cut(s) 205, 2000
BseRI GAGGAG 2 cut(s) 552, 2333
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 4 cut(s) 487, 815, 2300, 2330
BsgI GTGCAG 3 cut(s) 486, 843, 2152
Bsh1236I CGCG 1 cut(s) 2225
Bsh1285I CGRYCG 1 cut(s) 2309
BshFI GGCC 5 cut(s) 28, 64, 475, 1082, 1863
BshNI GGYRCC 1 cut(s) 2089
BsiEI CGRYCG 1 cut(s) 2309
BsiHKAI GWGCWC 2 cut(s) 1138, 2166
BsiHKCI CYCGRG 1 cut(s) 2026
BsiSI CCGG 5 cut(s) 248, 807, 1939, 2078, 2239
BslFI GGGAC 3 cut(s) 154, 951, 2244
BslI CCNNNNNNNGG 5 cut(s) 656, 872, 1283, 2189, 2268
BsmAI GTCTC 4 cut(s) 2, 1885, 1985, 2036
BsmFI GGGAC 3 cut(s) 154, 951, 2244
BsmI GAATGC 2 cut(s) 210, 961
BsnI GGCC 5 cut(s) 28, 64, 475, 1082, 1863
BsoBI CYCGRG 1 cut(s) 2026
Bsp1286I GDGCHC 3 cut(s) 951, 1138, 2166
Bsp13I TCCGGA 2 cut(s) 247, 2077
Bsp1720I GCTNAGC 1 cut(s) 1877
Bsp19I CCATGG 1 cut(s) 1005
Bsp68I TCGCGA 1 cut(s) 2225
BspACI CCGC 7 cut(s) 608, 742, 1096, 1144, 1530, 2297, 2332
BspANI GGCC 5 cut(s) 28, 64, 475, 1082, 1863
BspCNI CTCAG 6 cut(s) 893, 937, 1011, 1167, 1598, 2086
BspEI TCCGGA 2 cut(s) 247, 2077
BspFNI CGCG 1 cut(s) 2225
BspLI GGNNCC 7 cut(s) 106, 894, 967, 2091, 2236, 2260, 2295
BspMAI CTGCAG 3 cut(s) 676, 805, 2071
BspPI GGATC 9 cut(s) 97, 99, 112, 159, 887, 900, 1840, 2242, 2310
BspQI GCTCTTC 1 cut(s) 1383
BspT107I GGYRCC 1 cut(s) 2089
BsrDI GCAATG 5 cut(s) 1044, 1083, 1320, 1419, 1729
BsrI ACTGG 2 cut(s) 205, 2000
BssECI CCNNGG 7 cut(s) 774, 807, 931, 1005, 2143, 2183, 2263
BssSI CACGAG 5 cut(s) 12, 835, 1483, 1605, 1982
BssT1I CCWWGG 4 cut(s) 774, 1005, 2143, 2263
Bst2BI CACGAG 5 cut(s) 12, 835, 1483, 1605, 1982
Bst2UI CCWGG 2 cut(s) 750, 1565
Bst4CI ACNGT 6 cut(s) 616, 863, 902, 1597, 2002, 2184
Bst6I CTCTTC 1 cut(s) 1383
BstAPI GCANNNNNTGC 1 cut(s) 500
BstBAI YACGTR 1 cut(s) 1139
BstC8I GCNNGC 7 cut(s) 30, 1243, 1311, 1783, 1883, 1934, 2270
BstDEI CTNAG 9 cut(s) 880, 924, 1019, 1154, 1585, 1681, 1739, 1877, 2094
BstDSI CCRYGG 2 cut(s) 1005, 2183
BstF5I GGATG 5 cut(s) 1120, 1369, 1753, 1947, 2091
BstFNI CGCG 1 cut(s) 2225
BstMAI GTCTC 4 cut(s) 2, 1885, 1985, 2036
BstMCI CGRYCG 1 cut(s) 2309
BstMWI GCNNNNNNNGC 9 cut(s) 34, 311, 373, 500, 1150, 1310, 1555, 1882, 2340
BstNI CCWGG 2 cut(s) 750, 1565
BstNSI RCATGY 4 cut(s) 526, 1198, 1785, 1936
BstSCI CCNGG 4 cut(s) 748, 806, 1563, 2237
BstSFI CTRYAG 4 cut(s) 672, 801, 1210, 2067
BstSLI GKGCMC 1 cut(s) 951
BstUI CGCG 1 cut(s) 2225
BstV1I GCAGC 4 cut(s) 487, 815, 2300, 2330
BstV2I GAAGAC 1 cut(s) 1719
BstX2I RGATCY 8 cut(s) 89, 104, 267, 892, 1370, 1832, 2007, 2247
BstYI RGATCY 8 cut(s) 89, 104, 267, 892, 1370, 1832, 2007, 2247
BsuRI GGCC 5 cut(s) 28, 64, 475, 1082, 1863
BtgI CCRYGG 2 cut(s) 1005, 2183
BtsCI GGATG 5 cut(s) 1120, 1369, 1753, 1947, 2091
BtsIMutI CAGTG 4 cut(s) 907, 1162, 1993, 2118
BtuMI TCGCGA 1 cut(s) 2225
Cac8I GCNNGC 7 cut(s) 30, 1243, 1311, 1783, 1883, 1934, 2270
Cfr13I GGNCC 6 cut(s) 26, 965, 1146, 1469, 1501, 2258
Csp6I GTAC 3 cut(s) 129, 1441, 2350
CviQI GTAC 3 cut(s) 129, 1441, 2350
DdeI CTNAG 9 cut(s) 880, 924, 1019, 1154, 1585, 1681, 1739, 1877, 2094
DraIII CACNNNGTG 1 cut(s) 1034
DriI GACNNNNNGTC 1 cut(s) 2062
EaeI YGGCCR 2 cut(s) 1080, 1861
Eam1104I CTCTTC 1 cut(s) 1383
Eam1105I GACNNNNNGTC 1 cut(s) 2062
EarI CTCTTC 1 cut(s) 1383
EciI GGCGGA 2 cut(s) 623, 1519
Ecl136II GAGCTC 1 cut(s) 2164
Eco130I CCWWGG 4 cut(s) 774, 1005, 2143, 2263
Eco24I GRGCYC 1 cut(s) 2166
Eco32I GATATC 2 cut(s) 1294, 1731
Eco47I GGWCC 5 cut(s) 965, 1146, 1469, 1501, 2258
Eco53kI GAGCTC 1 cut(s) 2164
Eco57I CTGAAG 4 cut(s) 51, 398, 1364, 1458
Eco72I CACGTG 1 cut(s) 1139
Eco88I CYCGRG 1 cut(s) 2026
EcoICRI GAGCTC 1 cut(s) 2164
EcoO109I RGGNCCY 2 cut(s) 965, 2258
EcoRI GAATTC 1 cut(s) 1518
EcoRII CCWGG 2 cut(s) 748, 1563
EcoRV GATATC 2 cut(s) 1294, 1731
EcoT14I CCWWGG 4 cut(s) 774, 1005, 2143, 2263
EcoT38I GRGCYC 1 cut(s) 2166
ErhI CCWWGG 4 cut(s) 774, 1005, 2143, 2263
FalI AAGNNNNNCTT 8 cut(s) 254, 286, 489, 521, 1438, 1470, 1664, 1696
FaqI GGGAC 3 cut(s) 154, 951, 2244
FauI CCCGC 1 cut(s) 2339
FauNDI CATATG 2 cut(s) 690, 1913
Fnu4HI GCNGC 4 cut(s) 501, 804, 2314, 2344
FokI GGATG 5 cut(s) 1107, 1376, 1740, 1954, 2098
FriOI GRGCYC 1 cut(s) 2166
Fsp4HI GCNGC 4 cut(s) 501, 804, 2314, 2344
FspBI CTAG 5 cut(s) 141, 582, 851, 1104, 2264
GluI GCNGC 4 cut(s) 501, 804, 2314, 2344
GsuI CTGGAG 2 cut(s) 1260, 1544
HaeIII GGCC 5 cut(s) 28, 64, 475, 1082, 1863
HapII CCGG 5 cut(s) 248, 807, 1939, 2078, 2239
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 1874
HpaII CCGG 5 cut(s) 248, 807, 1939, 2078, 2239
HphI GGTGA 6 cut(s) 129, 412, 837, 1172, 1298, 1370
Hpy166II GTNNAC 5 cut(s) 79, 733, 1037, 1482, 2215
Hpy8I GTNNAC 5 cut(s) 79, 733, 1037, 1482, 2215
HpyCH4III ACNGT 6 cut(s) 616, 863, 902, 1597, 2002, 2184
HpyCH4IV ACGT 1 cut(s) 1138
HpyF10VI GCNNNNNNNGC 9 cut(s) 34, 311, 373, 500, 1150, 1310, 1555, 1882, 2340
HpyF3I CTNAG 9 cut(s) 880, 924, 1019, 1154, 1585, 1681, 1739, 1877, 2094
HpySE526I ACGT 1 cut(s) 1138
Kpn2I TCCGGA 2 cut(s) 247, 2077
LguI GCTCTTC 1 cut(s) 1383
LmnI GCTCC 1 cut(s) 2161
Lsp1109I GCAGC 4 cut(s) 487, 815, 2300, 2330
LweI GCATC 5 cut(s) 188, 729, 1129, 1235, 1762
MaeI CTAG 5 cut(s) 141, 582, 851, 1104, 2264
MaeII ACGT 1 cut(s) 1138
MaeIII GTNAC 4 cut(s) 759, 1810, 1907, 2012
MboII GAAGA 9 cut(s) 125, 521, 1266, 1269, 1370, 1724, 1879, 1882, 1957
MflI RGATCY 8 cut(s) 89, 104, 267, 892, 1370, 1832, 2007, 2247
MhlI GDGCHC 3 cut(s) 951, 1138, 2166
MlsI TGGCCA 1 cut(s) 1863
MluCI AATT 9 cut(s) 83, 722, 780, 996, 1464, 1496, 1518, 1589, 1975
MluNI TGGCCA 1 cut(s) 1863
MlyI GAGTC 6 cut(s) 669, 677, 827, 1535, 2016, 2051
MmeI TCCRAC 5 cut(s) 1052, 1579, 1591, 2223, 2267
Mox20I TGGCCA 1 cut(s) 1863
MroI TCCGGA 2 cut(s) 247, 2077
MroXI GAANNNNTTC 3 cut(s) 219, 1635, 2193
MscI TGGCCA 1 cut(s) 1863
MseI TTAA 2 cut(s) 1250, 2153
MslI CAYNNNNRTG 3 cut(s) 1758, 1826, 1857
Msp20I TGGCCA 1 cut(s) 1863
MspA1I CMGCKG 2 cut(s) 425, 1096
MspI CCGG 5 cut(s) 248, 807, 1939, 2078, 2239
MspR9I CCNGG 4 cut(s) 750, 808, 1565, 2239
Mva1269I GAATGC 2 cut(s) 210, 961
MvaI CCWGG 2 cut(s) 750, 1565
MvnI CGCG 1 cut(s) 2225
MwoI GCNNNNNNNGC 9 cut(s) 34, 311, 373, 500, 1150, 1310, 1555, 1882, 2340
NciI CCSGG 2 cut(s) 808, 2239
NcoI CCATGG 1 cut(s) 1005
NdeI CATATG 2 cut(s) 690, 1913
NlaIV GGNNCC 7 cut(s) 106, 894, 967, 2091, 2236, 2260, 2295
NmuCI GTSAC 2 cut(s) 1907, 2012
NruI TCGCGA 1 cut(s) 2225
NspI RCATGY 4 cut(s) 526, 1198, 1785, 1936
PaeI GCATGC 2 cut(s) 1785, 1936
PaeR7I CTCGAG 1 cut(s) 2026
PciI ACATGT 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 1383
PctI GAATGC 2 cut(s) 210, 961
PdmI GAANNNNTTC 3 cut(s) 219, 1635, 2193
PfeI GAWTC 9 cut(s) 116, 134, 220, 532, 677, 842, 876, 1336, 1849
PflMI CCANNNNNTGG 1 cut(s) 1283
PkrI GCNGC 4 cut(s) 502, 805, 2315, 2345
PleI GAGTC 6 cut(s) 668, 676, 827, 1534, 2016, 2051
PmaCI CACGTG 1 cut(s) 1139
PmlI CACGTG 1 cut(s) 1139
PpsI GAGTC 6 cut(s) 668, 676, 827, 1534, 2016, 2051
Ppu21I YACGTR 1 cut(s) 1139
PpuMI RGGWCCY 2 cut(s) 965, 2258
PscI ACATGT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 2166
Psp5II RGGWCCY 2 cut(s) 965, 2258
Psp6I CCWGG 2 cut(s) 748, 1563
PspCI CACGTG 1 cut(s) 1139
PspGI CCWGG 2 cut(s) 748, 1563
PspN4I GGNNCC 7 cut(s) 106, 894, 967, 2091, 2236, 2260, 2295
PspPI GGNCC 6 cut(s) 26, 965, 1146, 1469, 1501, 2258
PspPPI RGGWCCY 2 cut(s) 965, 2258
PstI CTGCAG 3 cut(s) 676, 805, 2071
PsuI RGATCY 8 cut(s) 89, 104, 267, 892, 1370, 1832, 2007, 2247
PvuII CAGCTG 1 cut(s) 425
RruI TCGCGA 1 cut(s) 2225
RsaI GTAC 3 cut(s) 130, 1442, 2351
RsaNI GTAC 3 cut(s) 129, 1441, 2350
RseI CAYNNNNRTG 3 cut(s) 1758, 1826, 1857
SacI GAGCTC 1 cut(s) 2166
SapI GCTCTTC 1 cut(s) 1383
SaqAI TTAA 2 cut(s) 1250, 2153
SatI GCNGC 4 cut(s) 501, 804, 2314, 2344
Sau96I GGNCC 6 cut(s) 26, 965, 1146, 1469, 1501, 2258
ScaI AGTACT 2 cut(s) 130, 2351
SchI GAGTC 6 cut(s) 669, 677, 827, 1535, 2016, 2051
ScrFI CCNGG 4 cut(s) 750, 808, 1565, 2239
SduI GDGCHC 3 cut(s) 951, 1138, 2166
SfaNI GCATC 5 cut(s) 188, 729, 1129, 1235, 1762
SfcI CTRYAG 4 cut(s) 672, 801, 1210, 2067
Sfr274I CTCGAG 1 cut(s) 2026
SinI GGWCC 5 cut(s) 965, 1146, 1469, 1501, 2258
SlaI CTCGAG 1 cut(s) 2026
SmiMI CAYNNNNRTG 3 cut(s) 1758, 1826, 1857
SmlI CTYRAG 4 cut(s) 260, 308, 1204, 2026
SmoI CTYRAG 4 cut(s) 260, 308, 1204, 2026
SphI GCATGC 2 cut(s) 1785, 1936
Sse9I AATT 9 cut(s) 83, 722, 780, 996, 1464, 1496, 1518, 1589, 1975
SsiI CCGC 7 cut(s) 608, 742, 1096, 1144, 1530, 2297, 2332
SspI AATATT 1 cut(s) 1576
SspMI CTAG 5 cut(s) 141, 582, 851, 1104, 2264
SstI GAGCTC 1 cut(s) 2166
StyD4I CCNGG 4 cut(s) 748, 806, 1563, 2237
StyI CCWWGG 4 cut(s) 774, 1005, 2143, 2263
TaaI ACNGT 6 cut(s) 616, 863, 902, 1597, 2002, 2184
TaiI ACGT 1 cut(s) 1141
TaqI TCGA 6 cut(s) 535, 953, 1339, 1639, 2027, 2305
TaqII GACCGA 1 cut(s) 2323
TasI AATT 9 cut(s) 83, 722, 780, 996, 1464, 1496, 1518, 1589, 1975
TatI WGTACW 3 cut(s) 128, 1440, 2349
TfiI GAWTC 9 cut(s) 116, 134, 220, 532, 677, 842, 876, 1336, 1849
Tru1I TTAA 2 cut(s) 1250, 2153
Tru9I TTAA 2 cut(s) 1250, 2153
TscAI CASTG 4 cut(s) 907, 1162, 2000, 2125
TseFI GTSAC 2 cut(s) 1907, 2012
TseI GCWGC 4 cut(s) 500, 803, 2313, 2343
Tsp45I GTSAC 2 cut(s) 1907, 2012
TspGWI ACGGA 1 cut(s) 2306
TspRI CASTG 4 cut(s) 907, 1162, 2000, 2125
Van91I CCANNNNNTGG 1 cut(s) 1283
VpaK11BI GGWCC 5 cut(s) 965, 1146, 1469, 1501, 2258
XapI RAATTY 2 cut(s) 83, 1518
XceI RCATGY 4 cut(s) 526, 1198, 1785, 1936
XcmI CCANNNNNNNNNTGG 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 2026
XmaJI CCTAGG 1 cut(s) 2263
XmnI GAANNNNTTC 3 cut(s) 219, 1635, 2193
XspI CTAG 5 cut(s) 141, 582, 851, 1104, 2264
ZrmI AGTACT 2 cut(s) 130, 2351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.