pycom09g15530

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004364
Physical Location & Seq
Forward (+)
15420131 .. 15431055
10925 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g15530.9

Sequence Viewer

Length: 2262 bp
ATGGAGGAGACACGAGATGATACAGGGCCAGCAGAGCAAAGGCCTCCAAATGCTTCATGGTGGCCTTCAGATTTCGTGGACAAATTTGGATCTGTTTCTTTGGGATCCCAAGAAGAATCATTGAGTAGTACTGAATCACCTAGAAGTTCAGATCAAGATGTGTTGTCATTCCAGAAGGCATCACAAGTTGTATGGCAAACTGGAATGCTTTTAGAACCTATTCCCAATGGTTTCTATTCTGTCATTCCGGAGAAAAGGCTCAAGGAGATCTTTGATGATATTCCTACTCTTGATGAGCTTCATGCTCTTGAGGCAGAGGGTTTGAAAGCCGATGTCATACTTGTAGATACTGCAAAAGACAAAAAGCTGTCCATGCTGAAGCAACTTATTGTGGCTCTGGTGAAAGGATTGAACTCAAATCCAGCTGTAATGATAAAAAAGATTGCTGGATTGGTTTCCGATTTTTATAAGAGGCCAAATGCAGAAAGTCCAGCAAAAGCTGCACTTGAAGAAACCTCCCACATGTCTGAGAATCGAGGAGTTCAACTTTTGGGGCAAATAAAACATGGTTCATGCCGTCCTAGAGCAATCTTATTCAAAGTTCTGGCAGACACCGTAGGTCTTGAAAGTAGGCTAATGGTGGGATTGCCTATTGATGGAGCCGTTGAGTCTGTAGATTCATATAAGCATATGTCGGTGGTAGTTGTATTGAACTCTGTTGAATTATTGGTTGACCTGATGCGGTTTCCTGGTAAGCTGTTACCCAGATCGACCAAGGCAATTTTTTTGACCCATATTTCTGCAGCTGGGGAGAGTGATAGTGCAGAGAATGACTCGTGTGATTCACCACTAGAACCGAACAGTCCTCTATATGGATTCTCAGAGAGAGTGGATCCTGACAGTGCTGAAAAAGATGATAGCCCTCAGTTCCGAGGTAGATTTGATGTGCCCTCGAATGCACAAGGTCCCTCATTACGAAATATGATGTTGCGACCAATTACTTCCATGGAAGGAAAACTGAGTTTATCACATAGTGAACCCAACATTGCAACTACATTTTGGCGGCGTAGTCGGAGGACGGCCATTGCTGAACAGCGGACTGCTAGTTCAAGGTCAGATGGTGCATCAACATCAGAAGCTCGCAGATTAAGAAGAAGAAGCATGAGTATTACTCCAGAGATTGGTGATGATATCGTAAGGGCTGTACGAGCGATGAATGAAACATTGAAGCAGAATCGACTTTTGAGAGAGCAGGGTGAGGATGACAGATCTGGTCTTCAGAAAAATGATCAAATCAGTTCTCAAAAAGCAATGTCATTACCTTCATCTCCTCATCAGTACAGAAGTCAAACGTCTGATAGAATTGGACCTTCAGAGTTCACGACATATGATGAATTGGTCCTAACATGGAACAGAATTCTGGAGTCCGCCACATTTGATAATAAGCCTCTATTGCCTTACCAGGAATGGAATATTGATTTCTCAGAATTGACTGTTGGAACTCGTGTTGGAATAGGGTTCTTTGGAGAAGTCTTTCGAGGCGTTTGGAATGGAACAGATGTCGCAGTAAAGGTTTTCTTAGAGCAAGACCTAACTGCTGAAAACATGGAAGACTTCTGCAATGAGATATCCATTCTTAGTCGCCTTCGGCATCCTAATGTTATTCTATTTCTGGGTGCATGCACAAAGCCTCCACGCTTATCAATGGTTACAGAATACATGGAGATGCGATCTTTGTATTATTTGATTCATTTGAGTGGCCAGAAGAAGAAGCTTAGCTGGCGAAGGAGACTAAAAATGTTGCGTGACATATGCAGGGGGTTGATGTGCATGCACCGGATGAAGATAGTTCATCGTGATCTAAAAAGTGCAAATTGTCTCGTGAATAAGCACTGGACTGTCAAGATCTGCGACTTTGGACTCTCGAGAATAATGTCCGAGACAAACATGAAGGACTCGTCGTCTGCAGGAACTCCGGAATGGATGGCACCTGAGCTTATACGAAATGAACCCTTCACCGAAAAATGTGATATTTTCAGCCTTGGGGTGTTAATGTGGGAGCTCTGCACTCTAAATAGACCGTGGGAAGGCGTTCCACCAGAGAGGGTAGTTTACGCTGTCGCGAATGAAGGTTCCCGGTTGGAGATCCCTGAAGGTCCGCTAGGCAGGCTTATTTCAGATTGTTGGACGGAACCGCAGGATCGACCGAGCTGCGAGGAGATACTCTCCCGCCTGTTAGACTGCGAGTACTCACTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 ko04013 ko04064 ko04140 ko04152 ko04214 ko04310 ko04380 ko04520 ko04620 ko04621 ko04622 ko04624 ko04657 ko04660 ko04668 ko05140 ko05145 ko05162 ko05168 ko05169 ko05418 map04010 map04013 map04064 map04140 map04152 map04214 map04310 map04380 map04520 map04620 map04621 map04622 map04624 map04657 map04660 map04668 map05140 map05145 map05162 map05168 map05169 map05418
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

754

Amino Acids

84.75

Weight (kDa)

5.7

Isoelectric Point (pI)

59.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 1987
AccB7I CCANNNNNTGG 1 cut(s) 1181
AccII CGCG 1 cut(s) 2123
AccIII TCCGGA 2 cut(s) 247, 1975
AciI CCGC 7 cut(s) 742, 1063, 1096, 1428, 2159, 2195, 2230
AclWI GGATC 7 cut(s) 97, 99, 112, 887, 900, 2140, 2208
AcoI YGGCCR 2 cut(s) 1080, 1759
AcsI RAATTY 2 cut(s) 83, 1416
AcuI CTGAAG 5 cut(s) 51, 398, 1262, 1356, 2172
AdeI CACNNNGTG 1 cut(s) 1034
AfaI GTAC 4 cut(s) 130, 1206, 1340, 2249
AfiI CCNNNNNNNGG 4 cut(s) 656, 872, 1181, 2087
AflIII ACRYGT 1 cut(s) 522
AhdI GACNNNNNGTC 1 cut(s) 1960
AjnI CCWGG 2 cut(s) 748, 1461
AjuI GAANNNNNNNTTGG 2 cut(s) 1479, 1511
AloI GAACNNNNNNTCC 2 cut(s) 1090, 1122
Alw21I GWGCWC 1 cut(s) 2064
Alw26I GTCTC 4 cut(s) 2, 1783, 1883, 1934
AlwI GGATC 7 cut(s) 97, 99, 112, 887, 900, 2140, 2208
Ama87I CYCGRG 1 cut(s) 1924
Aor13HI TCCGGA 2 cut(s) 247, 1975
AoxI GGCC 6 cut(s) 26, 41, 62, 473, 1080, 1759
ApeKI GCWGC 3 cut(s) 500, 803, 2211
ApoI RAATTY 2 cut(s) 83, 1416
Asp700I GAANNNNTTC 3 cut(s) 219, 1533, 2091
AspS9I GGNCC 5 cut(s) 26, 965, 1367, 1399, 2156
AsuC2I CCSGG 1 cut(s) 2137
AsuHPI GGTGA 6 cut(s) 129, 412, 837, 1196, 1268, 2008
AvaI CYCGRG 1 cut(s) 1924
AvaII GGWCC 4 cut(s) 965, 1367, 1399, 2156
BaeGI GKGCMC 1 cut(s) 951
BalI TGGCCA 1 cut(s) 1761
BamHI GGATCC 2 cut(s) 104, 892
BanI GGYRCC 1 cut(s) 1987
BanII GRGCYC 1 cut(s) 2064
BauI CACGAG 4 cut(s) 12, 835, 1503, 1880
BbsI GAAGAC 2 cut(s) 1268, 1617
Bbv12I GWGCWC 1 cut(s) 2064
BbvI GCAGC 3 cut(s) 487, 815, 2198
BccI CCATC 3 cut(s) 650, 1112, 1978
BceAI ACGGC 3 cut(s) 561, 647, 1095
BcgI CGANNNNNNTGC 2 cut(s) 2193, 2227
BciT130I CCWGG 2 cut(s) 750, 1463
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 1 cut(s) 2137
BcoDI GTCTC 4 cut(s) 2, 1783, 1883, 1934
BfaI CTAG 5 cut(s) 141, 582, 851, 1104, 2162
BfmI CTRYAG 3 cut(s) 672, 801, 1965
BglII AGATCT 3 cut(s) 267, 1268, 1905
BisI GCNGC 4 cut(s) 501, 804, 1064, 2212
BlpI GCTNAGC 1 cut(s) 1775
BlsI GCNGC 4 cut(s) 502, 805, 1065, 2213
BmcAI AGTACT 2 cut(s) 130, 2249
Bme1390I CCNGG 3 cut(s) 750, 1463, 2137
Bme18I GGWCC 4 cut(s) 965, 1367, 1399, 2156
BmeRI GACNNNNNGTC 1 cut(s) 1960
BmeT110I CYCGRG 1 cut(s) 1924
BmgT120I GGNCC 5 cut(s) 26, 965, 1367, 1399, 2156
BmiI GGNNCC 7 cut(s) 106, 661, 894, 967, 1989, 2134, 2193
BmrFI CCNGG 3 cut(s) 750, 1463, 2137
BmsI GCATC 5 cut(s) 188, 729, 1133, 1660, 1716
BpiI GAAGAC 2 cut(s) 1268, 1617
BplI GAGNNNNNCTC 6 cut(s) 818, 850, 1156, 1188, 2210, 2242
BpmI CTGGAG 2 cut(s) 1158, 1442
Bpu10I CCTNAGC 1 cut(s) 1992
Bpu1102I GCTNAGC 1 cut(s) 1775
BpuEI CTTGAG 2 cut(s) 245, 329
BpuMI CCSGG 1 cut(s) 2137
BsaJI CCNNGG 5 cut(s) 774, 931, 1005, 2041, 2081
BsaWI WCCGGW 3 cut(s) 247, 1836, 1975
BsaXI ACNNNNNCTCC 2 cut(s) 1675, 1705
Bsc4I CCNNNNNNNGG 4 cut(s) 656, 872, 1181, 2087
Bse1I ACTGG 2 cut(s) 205, 1898
Bse3DI GCAATG 4 cut(s) 1044, 1083, 1317, 1627
BseAI TCCGGA 2 cut(s) 247, 1975
BseBI CCWGG 2 cut(s) 750, 1463
BseDI CCNNGG 5 cut(s) 774, 931, 1005, 2041, 2081
BseGI GGATG 4 cut(s) 1267, 1651, 1845, 1989
BseLI CCNNNNNNNGG 4 cut(s) 656, 872, 1181, 2087
BseMI GCAATG 4 cut(s) 1044, 1083, 1317, 1627
BseMII CTCAG 6 cut(s) 519, 894, 938, 1010, 1497, 1983
BseNI ACTGG 2 cut(s) 205, 1898
BseRI GAGGAG 4 cut(s) 20, 552, 1320, 2231
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 3 cut(s) 487, 815, 2198
BseYI CCCAGC 1 cut(s) 806
BsgI GTGCAG 3 cut(s) 486, 843, 2050
Bsh1236I CGCG 1 cut(s) 2123
Bsh1285I CGRYCG 1 cut(s) 2207
BshFI GGCC 6 cut(s) 28, 43, 64, 475, 1082, 1761
BshNI GGYRCC 1 cut(s) 1987
BsiEI CGRYCG 1 cut(s) 2207
BsiHKAI GWGCWC 1 cut(s) 2064
BsiHKCI CYCGRG 1 cut(s) 1924
BsiSI CCGG 4 cut(s) 248, 1837, 1976, 2137
BslFI GGGAC 1 cut(s) 951
BslI CCNNNNNNNGG 4 cut(s) 656, 872, 1181, 2087
BsmAI GTCTC 4 cut(s) 2, 1783, 1883, 1934
BsmFI GGGAC 1 cut(s) 951
BsmI GAATGC 2 cut(s) 210, 961
BsnI GGCC 6 cut(s) 28, 43, 64, 475, 1082, 1761
BsoBI CYCGRG 1 cut(s) 1924
Bsp1286I GDGCHC 2 cut(s) 951, 2064
Bsp13I TCCGGA 2 cut(s) 247, 1975
Bsp1720I GCTNAGC 1 cut(s) 1775
Bsp19I CCATGG 1 cut(s) 1005
Bsp68I TCGCGA 1 cut(s) 2123
BspACI CCGC 7 cut(s) 742, 1063, 1096, 1428, 2159, 2195, 2230
BspANI GGCC 6 cut(s) 28, 43, 64, 475, 1082, 1761
BspCNI CTCAG 6 cut(s) 520, 893, 937, 1011, 1496, 1984
BspEI TCCGGA 2 cut(s) 247, 1975
BspFNI CGCG 1 cut(s) 2123
BspLI GGNNCC 7 cut(s) 106, 661, 894, 967, 1989, 2134, 2193
BspMAI CTGCAG 2 cut(s) 805, 1969
BspPI GGATC 7 cut(s) 97, 99, 112, 887, 900, 2140, 2208
BspT107I GGYRCC 1 cut(s) 1987
BsrDI GCAATG 4 cut(s) 1044, 1083, 1317, 1627
BsrI ACTGG 2 cut(s) 205, 1898
BssECI CCNNGG 5 cut(s) 774, 931, 1005, 2041, 2081
BssSI CACGAG 4 cut(s) 12, 835, 1503, 1880
BssT1I CCWWGG 3 cut(s) 774, 1005, 2041
Bst2BI CACGAG 4 cut(s) 12, 835, 1503, 1880
Bst2UI CCWGG 2 cut(s) 750, 1463
Bst4CI ACNGT 6 cut(s) 616, 863, 902, 1495, 1900, 2082
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 6 cut(s) 30, 1141, 1681, 1781, 1832, 2168
BstDEI CTNAG 9 cut(s) 528, 880, 924, 1019, 1483, 1579, 1637, 1775, 1992
BstDSI CCRYGG 2 cut(s) 1005, 2081
BstF5I GGATG 4 cut(s) 1267, 1651, 1845, 1989
BstFNI CGCG 1 cut(s) 2123
BstMAI GTCTC 4 cut(s) 2, 1783, 1883, 1934
BstMCI CGRYCG 1 cut(s) 2207
BstMWI GCNNNNNNNGC 8 cut(s) 34, 311, 373, 500, 1208, 1453, 1780, 2167
BstNI CCWGG 2 cut(s) 750, 1463
BstNSI RCATGY 3 cut(s) 526, 1683, 1834
BstSCI CCNGG 3 cut(s) 748, 1461, 2135
BstSFI CTRYAG 3 cut(s) 672, 801, 1965
BstSLI GKGCMC 1 cut(s) 951
BstUI CGCG 1 cut(s) 2123
BstV1I GCAGC 3 cut(s) 487, 815, 2198
BstV2I GAAGAC 2 cut(s) 1268, 1617
BstX2I RGATCY 7 cut(s) 89, 104, 267, 892, 1268, 1905, 2145
BstYI RGATCY 7 cut(s) 89, 104, 267, 892, 1268, 1905, 2145
BsuRI GGCC 6 cut(s) 28, 43, 64, 475, 1082, 1761
BtgI CCRYGG 2 cut(s) 1005, 2081
BtgZI GCGATG 1 cut(s) 1226
BtsCI GGATG 4 cut(s) 1267, 1651, 1845, 1989
BtsIMutI CAGTG 2 cut(s) 907, 1891
BtuMI TCGCGA 1 cut(s) 2123
Cac8I GCNNGC 6 cut(s) 30, 1141, 1681, 1781, 1832, 2168
Cfr13I GGNCC 5 cut(s) 26, 965, 1367, 1399, 2156
Csp6I GTAC 4 cut(s) 129, 1205, 1339, 2248
CviQI GTAC 4 cut(s) 129, 1205, 1339, 2248
DdeI CTNAG 9 cut(s) 528, 880, 924, 1019, 1483, 1579, 1637, 1775, 1992
DraIII CACNNNGTG 1 cut(s) 1034
DriI GACNNNNNGTC 1 cut(s) 1960
EaeI YGGCCR 2 cut(s) 1080, 1759
Eam1105I GACNNNNNGTC 1 cut(s) 1960
EciI GGCGGA 1 cut(s) 1417
Ecl136II GAGCTC 1 cut(s) 2062
Eco130I CCWWGG 3 cut(s) 774, 1005, 2041
Eco147I AGGCCT 1 cut(s) 43
Eco24I GRGCYC 1 cut(s) 2064
Eco32I GATATC 2 cut(s) 1192, 1629
Eco47I GGWCC 4 cut(s) 965, 1367, 1399, 2156
Eco53kI GAGCTC 1 cut(s) 2062
Eco57I CTGAAG 5 cut(s) 51, 398, 1262, 1356, 2172
Eco88I CYCGRG 1 cut(s) 1924
EcoICRI GAGCTC 1 cut(s) 2062
EcoO109I RGGNCCY 1 cut(s) 965
EcoRI GAATTC 1 cut(s) 1416
EcoRII CCWGG 2 cut(s) 748, 1461
EcoRV GATATC 2 cut(s) 1192, 1629
EcoT14I CCWWGG 3 cut(s) 774, 1005, 2041
EcoT38I GRGCYC 1 cut(s) 2064
ErhI CCWWGG 3 cut(s) 774, 1005, 2041
FalI AAGNNNNNCTT 6 cut(s) 254, 286, 489, 521, 1562, 1594
FaqI GGGAC 1 cut(s) 951
FauI CCCGC 1 cut(s) 2237
FauNDI CATATG 3 cut(s) 690, 1387, 1811
FbaI TGATCA 1 cut(s) 1288
Fnu4HI GCNGC 4 cut(s) 501, 804, 1064, 2212
FokI GGATG 4 cut(s) 1274, 1638, 1852, 1996
FriOI GRGCYC 1 cut(s) 2064
Fsp4HI GCNGC 4 cut(s) 501, 804, 1064, 2212
FspBI CTAG 5 cut(s) 141, 582, 851, 1104, 2162
GluI GCNGC 4 cut(s) 501, 804, 1064, 2212
GsaI CCCAGC 1 cut(s) 810
GsuI CTGGAG 2 cut(s) 1158, 1442
HaeIII GGCC 6 cut(s) 28, 43, 64, 475, 1082, 1761
HapII CCGG 4 cut(s) 248, 1837, 1976, 2137
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 1772
HpaII CCGG 4 cut(s) 248, 1837, 1976, 2137
HphI GGTGA 6 cut(s) 129, 412, 837, 1196, 1268, 2008
Hpy166II GTNNAC 5 cut(s) 79, 733, 1037, 1380, 2113
Hpy8I GTNNAC 5 cut(s) 79, 733, 1037, 1380, 2113
Hpy99I CGWCG 1 cut(s) 1963
HpyCH4III ACNGT 6 cut(s) 616, 863, 902, 1495, 1900, 2082
HpyCH4IV ACGT 1 cut(s) 1352
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 311, 373, 500, 1208, 1453, 1780, 2167
HpyF3I CTNAG 9 cut(s) 528, 880, 924, 1019, 1483, 1579, 1637, 1775, 1992
HpySE526I ACGT 1 cut(s) 1352
Kpn2I TCCGGA 2 cut(s) 247, 1975
Ksp22I TGATCA 1 cut(s) 1288
LmnI GCTCC 2 cut(s) 659, 2059
Lsp1109I GCAGC 3 cut(s) 487, 815, 2198
LweI GCATC 5 cut(s) 188, 729, 1133, 1660, 1716
MaeI CTAG 5 cut(s) 141, 582, 851, 1104, 2162
MaeII ACGT 1 cut(s) 1352
MaeIII GTNAC 3 cut(s) 759, 1708, 1805
MboII GAAGA 9 cut(s) 125, 521, 1164, 1167, 1268, 1622, 1777, 1780, 1855
MflI RGATCY 7 cut(s) 89, 104, 267, 892, 1268, 1905, 2145
MhlI GDGCHC 2 cut(s) 951, 2064
MlsI TGGCCA 1 cut(s) 1761
MluCI AATT 9 cut(s) 83, 722, 780, 996, 1362, 1394, 1416, 1487, 1873
MluNI TGGCCA 1 cut(s) 1761
MlyI GAGTC 5 cut(s) 677, 827, 1433, 1914, 1949
MmeI TCCRAC 5 cut(s) 1052, 1477, 1489, 2121, 2165
Mox20I TGGCCA 1 cut(s) 1761
MroI TCCGGA 2 cut(s) 247, 1975
MroXI GAANNNNTTC 3 cut(s) 219, 1533, 2091
MscI TGGCCA 1 cut(s) 1761
MseI TTAA 2 cut(s) 1148, 2051
MslI CAYNNNNRTG 3 cut(s) 1656, 1724, 1755
Msp20I TGGCCA 1 cut(s) 1761
MspA1I CMGCKG 3 cut(s) 425, 806, 1096
MspI CCGG 4 cut(s) 248, 1837, 1976, 2137
MspR9I CCNGG 3 cut(s) 750, 1463, 2137
Mva1269I GAATGC 2 cut(s) 210, 961
MvaI CCWGG 2 cut(s) 750, 1463
MvnI CGCG 1 cut(s) 2123
MwoI GCNNNNNNNGC 8 cut(s) 34, 311, 373, 500, 1208, 1453, 1780, 2167
NciI CCSGG 1 cut(s) 2137
NcoI CCATGG 1 cut(s) 1005
NdeI CATATG 3 cut(s) 690, 1387, 1811
NlaIV GGNNCC 7 cut(s) 106, 661, 894, 967, 1989, 2134, 2193
NmuCI GTSAC 1 cut(s) 1805
NruI TCGCGA 1 cut(s) 2123
NspI RCATGY 3 cut(s) 526, 1683, 1834
PaeI GCATGC 2 cut(s) 1683, 1834
PaeR7I CTCGAG 1 cut(s) 1924
PceI AGGCCT 1 cut(s) 43
PciI ACATGT 1 cut(s) 522
PctI GAATGC 2 cut(s) 210, 961
PdmI GAANNNNTTC 3 cut(s) 219, 1533, 2091
PfeI GAWTC 8 cut(s) 116, 134, 532, 677, 842, 876, 1234, 1747
PflMI CCANNNNNTGG 1 cut(s) 1181
PkrI GCNGC 4 cut(s) 502, 805, 1065, 2213
PleI GAGTC 5 cut(s) 676, 827, 1432, 1914, 1949
PpsI GAGTC 5 cut(s) 676, 827, 1432, 1914, 1949
PpuMI RGGWCCY 1 cut(s) 965
PscI ACATGT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 2064
Psp5II RGGWCCY 1 cut(s) 965
Psp6I CCWGG 2 cut(s) 748, 1461
PspFI CCCAGC 1 cut(s) 806
PspGI CCWGG 2 cut(s) 748, 1461
PspN4I GGNNCC 7 cut(s) 106, 661, 894, 967, 1989, 2134, 2193
PspPI GGNCC 5 cut(s) 26, 965, 1367, 1399, 2156
PspPPI RGGWCCY 1 cut(s) 965
PstI CTGCAG 2 cut(s) 805, 1969
PsuI RGATCY 7 cut(s) 89, 104, 267, 892, 1268, 1905, 2145
PvuII CAGCTG 2 cut(s) 425, 806
RruI TCGCGA 1 cut(s) 2123
RsaI GTAC 4 cut(s) 130, 1206, 1340, 2249
RsaNI GTAC 4 cut(s) 129, 1205, 1339, 2248
RseI CAYNNNNRTG 3 cut(s) 1656, 1724, 1755
SacI GAGCTC 1 cut(s) 2064
SaqAI TTAA 2 cut(s) 1148, 2051
SatI GCNGC 4 cut(s) 501, 804, 1064, 2212
Sau96I GGNCC 5 cut(s) 26, 965, 1367, 1399, 2156
ScaI AGTACT 2 cut(s) 130, 2249
SchI GAGTC 5 cut(s) 677, 827, 1433, 1914, 1949
ScrFI CCNGG 3 cut(s) 750, 1463, 2137
SduI GDGCHC 2 cut(s) 951, 2064
SfaNI GCATC 5 cut(s) 188, 729, 1133, 1660, 1716
SfcI CTRYAG 3 cut(s) 672, 801, 1965
Sfr274I CTCGAG 1 cut(s) 1924
SinI GGWCC 4 cut(s) 965, 1367, 1399, 2156
SlaI CTCGAG 1 cut(s) 1924
SmiMI CAYNNNNRTG 3 cut(s) 1656, 1724, 1755
SmlI CTYRAG 3 cut(s) 260, 308, 1924
SmoI CTYRAG 3 cut(s) 260, 308, 1924
SphI GCATGC 2 cut(s) 1683, 1834
Sse9I AATT 9 cut(s) 83, 722, 780, 996, 1362, 1394, 1416, 1487, 1873
SseBI AGGCCT 1 cut(s) 43
SsiI CCGC 7 cut(s) 742, 1063, 1096, 1428, 2159, 2195, 2230
SspI AATATT 1 cut(s) 1474
SspMI CTAG 5 cut(s) 141, 582, 851, 1104, 2162
SstI GAGCTC 1 cut(s) 2064
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 3 cut(s) 748, 1461, 2135
StyI CCWWGG 3 cut(s) 774, 1005, 2041
TaaI ACNGT 6 cut(s) 616, 863, 902, 1495, 1900, 2082
TaiI ACGT 1 cut(s) 1355
TaqI TCGA 7 cut(s) 535, 770, 953, 1237, 1537, 1925, 2203
TaqII GACCGA 1 cut(s) 2221
TasI AATT 9 cut(s) 83, 722, 780, 996, 1362, 1394, 1416, 1487, 1873
TatI WGTACW 3 cut(s) 128, 1338, 2247
TauI GCSGC 1 cut(s) 1066
TfiI GAWTC 8 cut(s) 116, 134, 532, 677, 842, 876, 1234, 1747
Tru1I TTAA 2 cut(s) 1148, 2051
Tru9I TTAA 2 cut(s) 1148, 2051
TscAI CASTG 2 cut(s) 907, 1898
TseFI GTSAC 1 cut(s) 1805
TseI GCWGC 3 cut(s) 500, 803, 2211
Tsp45I GTSAC 1 cut(s) 1805
TspGWI ACGGA 1 cut(s) 2204
TspRI CASTG 2 cut(s) 907, 1898
Van91I CCANNNNNTGG 1 cut(s) 1181
VpaK11BI GGWCC 4 cut(s) 965, 1367, 1399, 2156
XapI RAATTY 2 cut(s) 83, 1416
XceI RCATGY 3 cut(s) 526, 1683, 1834
XcmI CCANNNNNNNNNTGG 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 1924
XmnI GAANNNNTTC 3 cut(s) 219, 1533, 2091
XspI CTAG 5 cut(s) 141, 582, 851, 1104, 2162
ZrmI AGTACT 2 cut(s) 130, 2249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.