Prupe.3G117600_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0004364
Physical Location & Seq
Forward (+)
10047224 .. 10062479
15256 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G117600.2

Sequence Viewer

Length: 2373 bp
ATGGAGGAGACACGAGATGATGCAGGGCCCGCAGAGCAAGGGCCTCCAAATGCTTCATGGTGGCCTTCAGATTTCGTGGACAAATTTGGATCTGTTTCTTTGGGTTCCCATGAAGAAACCTTGAGTAATACCGAATCACCTAGAAGTCCGTACCAAGATGTGTTGTCATCTCAGACAGCATCACAAGTTCTATGGCAAAGTGGAATGCTTTTAGAACCGATTCCCAATGGTTTCTATTCTGTCATTCCGGATAAAAGGCTCAAGGAGATCTTTGATGATATTCCTACTCTTGATGAACTTCATGCAATTGAGGCAGAGGGTGTGAAAGCTGATGTCATTCTTGTAGATAGTTCAAAAGACAAAAAGTTGTCCATGCTGAAGCAACTGATTGTGGCACTAGTGAAAGGATTGAACTCAAATCCAGCTGCAATGATAAAAAAGATTGCTGGACTGGTTTCAGATTTTTATAAGCGGCCACCTGTGGAAAGTCCAGCAAAAGCTGCACTAGAAGAAACGTCCCACATGTTTGATAATCGAGGAGTCCAACTTTTGGGGCAAATAAAGCATGGTTCATGCCGTCCTAGAGCAATCCTGTTCAAAGTTCTAGCAGATACCGTAGGTCTTGAAAGTAGGCTAGTGGTGGGTCTTTCTATTGATGGGGCTGTTGAGTGTGTAGATTCATATAAGCATATGTCTGTGATAGTTGTGTTGAACTCTGTTGAACTATTGGTCGACCTAATGCGGATCCCCGGCAAGCTGTTACCCCGATCAACCAAGGCAATCTTTATGACCCATATTTCTGCAGCAGGGGAGAGTGATTCTGCGGAAAATGATTCCTGTGATTCACCACTAGAACCAAACAGTCCCCTATATGGATTTTCAGAGAGAATGGATCCTGACAGTGCTGAAAGAGATGAGAGTCTTCAGTTCCGAGGGAGATTTGATGTGCCCTCGAATGTACAAGGTCCTTCATTGCGAAATATGATGTTGCGACCTATCACTTCCATTGAAAGAAAACTGAGTTTATCACATAGTGAACCCAACATTGCAACTTCATTTTGGCGCCGTAGTCGAAGAAAGGCCATTGCTGAACAGCGGACTGCTAGTTCAAGTCCAGAGCATCCTTCATTTCGAGCACGTGGGCGGTCCATGCTCAGTGGTGATAGGAAATCTTTTAGAGATTATGCTGATGACATGCCTACCTCAAGCTACAGGTCAGATGGTACATCAACATCAGAAGCTCGTAGATTAAGAAGAAGAAGCATTAGTATTACTCCAGAGATTGGTGATGATATCGTAAGGGCTGTACGAGCGATGAATGAAACATTGAAGCAAAATCGTCTTTTGAGAGAGCAGGGTGATGATGACAGAAACATTACTTCTGGTCTTCAGAAAAATGACCAAATCAGTTCTCAAAAAGCAATATCGTTACCTTCATCACCACATCAATATAGGAGTCAAACGTCTGAGAGAATTGGACCTTCTGAATTCACGAGAAATGATGAATTGGTCTTAACATGGAACAGAATTCTTGAGTCCGCCACGTGTGGTAATAAACCTCTATTGCCTTATCAGGAATGGAATATTGATTTCTCAGAATTGACTGTTGGAACTCGTGTTGGAATTGGGTTCTTTGGAGAAGTCTTTCGAGGTGTTTGGAATGGAACAGATGTTGCAATAAAGGTTTTCTTAGAGCAAGATCTAACTACTGAAAACATGGAAGACTTCTGCAATGAGATATCCATTCTTAGCCGTCTTCGGCATCCAAATGTTATTTTATTTCTAGGTGCATGCACAAAGCCTCCACACTTATCAATGGTTACAGAATACATGGAGATGGGATCCTTGTATTATTTGATTCATTTAAGTGGCCAGAAGAAGAAGCTTAGCTGGCGAAGGAGACTAAAAATGTTGCGTGACATATGCAGGGGGTTGATGTGCATGCACCGGATGAAGATAGTTCACCGCGATTTAAAAAGTGCAAACTGTCTCGTGAATAAACATTGGACTGTCAAGATCTGTGATTTTGGGCTCTCAAGAATAATGAACGAGACAAACATGAAGGACTCCTCGTCTGCCGGAACTCCAGAATGGATGGCACCTGAACTTATTCGAAATGAACCCTTCACCGAAAAATGTGATATTTTCAGCCTCGGGGTGATAATGTGGGAGCTCTGCACTCTAAATAGACCGTGGGAAGGTGTTCCACCAGAGAGGGTAGTTTATGCTGTTGCCAATGAGGGTTCCCGATTGGAAATTCCGGACGGTCCACTGGGCAGGCTTATTTCGGATTGTTGGACAGAACCGGATGATCGGCCAAGCTGTGAGGAGATACTCTCCCGCTTGTTAGACTGCGAATACTCACTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 ko04013 ko04064 ko04140 ko04152 ko04214 ko04310 ko04380 ko04520 ko04620 ko04621 ko04622 ko04624 ko04657 ko04660 ko04668 ko05140 ko05145 ko05162 ko05168 ko05169 ko05418 map04010 map04013 map04064 map04140 map04152 map04214 map04310 map04380 map04520 map04620 map04621 map04622 map04624 map04657 map04660 map04668 map05140 map05145 map05162 map05168 map05169 map05418
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

791

Amino Acids

88.87

Weight (kDa)

5.89

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AccB1I GGYRCC 2 cut(s) 1062, 2098
AccB7I CCANNNNNTGG 2 cut(s) 550, 1283
AccI GTMKAC 1 cut(s) 732
AccII CGCG 1 cut(s) 1968
AccIII TCCGGA 2 cut(s) 247, 2260
AciI CCGC 9 cut(s) 30, 472, 742, 824, 1096, 1144, 1539, 1966, 2341
AclWI GGATC 7 cut(s) 97, 739, 752, 887, 900, 1836, 1849
AcoI YGGCCR 3 cut(s) 473, 1870, 2315
AcsI RAATTY 4 cut(s) 83, 1487, 1527, 2256
AcuI CTGAAG 4 cut(s) 51, 398, 908, 1373
AcvI CACGTG 2 cut(s) 1139, 1545
AcyI GRCGYC 1 cut(s) 1063
AdeI CACNNNGTG 1 cut(s) 1034
AfaI GTAC 4 cut(s) 152, 960, 1225, 1308
AfiI CCNNNNNNNGG 4 cut(s) 550, 872, 1283, 2198
AflIII ACRYGT 2 cut(s) 522, 1544
AgsI TTSAA 9 cut(s) 354, 412, 598, 626, 712, 722, 1010, 1110, 1330
AhdI GACNNNNNGTC 1 cut(s) 2071
AhlI ACTAGT 1 cut(s) 397
AjuI GAANNNNNNNTTGG 2 cut(s) 1590, 1622
AloI GAACNNNNNNTCC 2 cut(s) 1090, 1122
Alw21I GWGCWC 2 cut(s) 1138, 2175
Alw26I GTCTC 4 cut(s) 2, 1894, 1994, 2045
AlwI GGATC 7 cut(s) 97, 739, 752, 887, 900, 1836, 1849
Ama87I CYCGRG 1 cut(s) 2153
Aor13HI TCCGGA 2 cut(s) 247, 2260
AoxI GGCC 7 cut(s) 26, 41, 62, 473, 1080, 1870, 2315
ApaI GGGCCC 1 cut(s) 30
ApeKI GCWGC 3 cut(s) 425, 500, 803
ApoI RAATTY 4 cut(s) 83, 1487, 1527, 2256
Asp700I GAANNNNTTC 4 cut(s) 219, 1644, 2109, 2202
AspLEI GCGC 1 cut(s) 1065
AspS9I GGNCC 7 cut(s) 26, 27, 41, 965, 1146, 1478, 2267
AsuC2I CCSGG 1 cut(s) 750
AsuHPI GGTGA 9 cut(s) 129, 837, 1172, 1298, 1370, 1431, 1955, 2119, 2170
AsuII TTCGAA 1 cut(s) 2113
AvaI CYCGRG 1 cut(s) 2153
AvaII GGWCC 4 cut(s) 965, 1146, 1478, 2267
BaeGI GKGCMC 2 cut(s) 30, 951
BalI TGGCCA 1 cut(s) 1872
BamHI GGATCC 3 cut(s) 744, 892, 1841
BanI GGYRCC 2 cut(s) 1062, 2098
BanII GRGCYC 3 cut(s) 30, 2034, 2175
BauI CACGAG 4 cut(s) 12, 1492, 1614, 1991
BbrPI CACGTG 2 cut(s) 1139, 1545
BbsI GAAGAC 4 cut(s) 914, 1379, 1728, 1748
Bbv12I GWGCWC 2 cut(s) 1138, 2175
BbvI GCAGC 3 cut(s) 412, 487, 815
BccI CCATC 4 cut(s) 650, 1214, 1831, 2089
BceAI ACGGC 3 cut(s) 561, 1050, 1737
BcnI CCSGG 1 cut(s) 750
BcoDI GTCTC 4 cut(s) 2, 1894, 1994, 2045
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 9 cut(s) 141, 398, 506, 582, 605, 635, 851, 1104, 1784
BfmI CTRYAG 2 cut(s) 801, 1210
BfoI RGCGCY 1 cut(s) 1066
BglII AGATCT 3 cut(s) 267, 1699, 2016
BisI GCNGC 4 cut(s) 426, 473, 501, 804
BlpI GCTNAGC 1 cut(s) 1886
BlsI GCNGC 4 cut(s) 427, 474, 502, 805
Bme1390I CCNGG 1 cut(s) 750
Bme18I GGWCC 4 cut(s) 965, 1146, 1478, 2267
BmeRI GACNNNNNGTC 1 cut(s) 2071
BmeT110I CYCGRG 1 cut(s) 2153
BmgT120I GGNCC 7 cut(s) 26, 27, 41, 965, 1146, 1478, 2267
BmiI GGNNCC 8 cut(s) 28, 106, 746, 894, 1064, 1843, 2100, 2245
BmrFI CCNGG 1 cut(s) 750
BmrI ACTGGG 1 cut(s) 2282
BmsI GCATC 4 cut(s) 10, 188, 1129, 1771
BmuI ACTGGG 1 cut(s) 2282
BpiI GAAGAC 4 cut(s) 914, 1379, 1728, 1748
BplI GAGNNNNNCTC 2 cut(s) 2321, 2353
BpmI CTGGAG 2 cut(s) 1260, 2070
Bpu1102I GCTNAGC 1 cut(s) 1886
Bpu14I TTCGAA 1 cut(s) 2113
BpuEI CTTGAG 5 cut(s) 142, 245, 1189, 1553, 2020
BpuMI CCSGG 1 cut(s) 750
BsaAI YACGTR 2 cut(s) 1139, 1545
BsaHI GRCGYC 1 cut(s) 1063
BsaJI CCNNGG 5 cut(s) 748, 774, 931, 2152, 2192
BsaWI WCCGGW 4 cut(s) 247, 1947, 2260, 2305
BsaXI ACNNNNNCTCC 2 cut(s) 1786, 1816
Bsc4I CCNNNNNNNGG 4 cut(s) 550, 872, 1283, 2198
Bse1I ACTGG 2 cut(s) 456, 2277
Bse3DI GCAATG 5 cut(s) 435, 971, 1044, 1083, 1738
BseAI TCCGGA 2 cut(s) 247, 2260
BseDI CCNNGG 5 cut(s) 748, 774, 931, 2152, 2192
BseGI GGATG 5 cut(s) 1120, 1762, 1956, 2100, 2314
BseLI CCNNNNNNNGG 4 cut(s) 550, 872, 1283, 2198
BseMI GCAATG 5 cut(s) 435, 971, 1044, 1083, 1738
BseMII CTCAG 5 cut(s) 185, 1010, 1168, 1458, 1608
BseNI ACTGG 2 cut(s) 456, 2277
BseRI GAGGAG 4 cut(s) 20, 552, 2059, 2342
BseSI GKGCMC 2 cut(s) 30, 951
BseXI GCAGC 3 cut(s) 412, 487, 815
BsgI GTGCAG 2 cut(s) 486, 2161
Bsh1236I CGCG 1 cut(s) 1968
BshFI GGCC 7 cut(s) 28, 43, 64, 475, 1082, 1872, 2317
BshNI GGYRCC 2 cut(s) 1062, 2098
BsiHKAI GWGCWC 2 cut(s) 1138, 2175
BsiHKCI CYCGRG 1 cut(s) 2153
BsiSI CCGG 6 cut(s) 248, 750, 1948, 2079, 2261, 2306
BslFI GGGAC 2 cut(s) 502, 849
BslI CCNNNNNNNGG 4 cut(s) 550, 872, 1283, 2198
BsmAI GTCTC 4 cut(s) 2, 1894, 1994, 2045
BsmFI GGGAC 2 cut(s) 502, 849
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 7 cut(s) 28, 43, 64, 475, 1082, 1872, 2317
BsoBI CYCGRG 1 cut(s) 2153
Bsp119I TTCGAA 1 cut(s) 2113
Bsp120I GGGCCC 1 cut(s) 26
Bsp1286I GDGCHC 5 cut(s) 30, 951, 1138, 2034, 2175
Bsp13I TCCGGA 2 cut(s) 247, 2260
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
Bsp1720I GCTNAGC 1 cut(s) 1886
BspACI CCGC 9 cut(s) 30, 472, 742, 824, 1096, 1144, 1539, 1966, 2341
BspANI GGCC 7 cut(s) 28, 43, 64, 475, 1082, 1872, 2317
BspCNI CTCAG 5 cut(s) 184, 1011, 1167, 1459, 1607
BspEI TCCGGA 2 cut(s) 247, 2260
BspFNI CGCG 1 cut(s) 1968
BspLI GGNNCC 8 cut(s) 28, 106, 746, 894, 1064, 1843, 2100, 2245
BspMAI CTGCAG 1 cut(s) 805
BspPI GGATC 7 cut(s) 97, 739, 752, 887, 900, 1836, 1849
BspT104I TTCGAA 1 cut(s) 2113
BspT107I GGYRCC 2 cut(s) 1062, 2098
BsrDI GCAATG 5 cut(s) 435, 971, 1044, 1083, 1738
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 2 cut(s) 456, 2277
BssECI CCNNGG 5 cut(s) 748, 774, 931, 2152, 2192
BssMI GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
BssNI GRCGYC 1 cut(s) 1063
BssSI CACGAG 4 cut(s) 12, 1492, 1614, 1991
BssT1I CCWWGG 1 cut(s) 774
Bst2BI CACGAG 4 cut(s) 12, 1492, 1614, 1991
Bst4CI ACNGT 8 cut(s) 616, 863, 902, 1606, 1988, 2011, 2193, 2267
BstACI GRCGYC 1 cut(s) 1063
BstAPI GCANNNNNTGC 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 958
BstBAI YACGTR 2 cut(s) 1139, 1545
BstBI TTCGAA 1 cut(s) 2113
BstC8I GCNNGC 6 cut(s) 30, 755, 1792, 1892, 1943, 2279
BstDEI CTNAG 8 cut(s) 171, 1019, 1154, 1467, 1594, 1690, 1748, 1886
BstDSI CCRYGG 1 cut(s) 2192
BstF5I GGATG 5 cut(s) 1120, 1762, 1956, 2100, 2314
BstFNI CGCG 1 cut(s) 1968
BstH2I RGCGCY 1 cut(s) 1066
BstHHI GCGC 1 cut(s) 1065
BstKTI GATC 9 cut(s) 92, 270, 747, 770, 895, 1702, 1844, 2019, 2314
BstMAI GTCTC 4 cut(s) 2, 1894, 1994, 2045
BstMBI GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
BstMWI GCNNNNNNNGC 8 cut(s) 29, 34, 311, 500, 562, 1150, 1310, 1891
BstNSI RCATGY 4 cut(s) 526, 1198, 1794, 1945
BstSCI CCNGG 1 cut(s) 748
BstSFI CTRYAG 2 cut(s) 801, 1210
BstSLI GKGCMC 2 cut(s) 30, 951
BstUI CGCG 1 cut(s) 1968
BstV1I GCAGC 3 cut(s) 412, 487, 815
BstV2I GAAGAC 4 cut(s) 914, 1379, 1728, 1748
BstX2I RGATCY 7 cut(s) 89, 267, 744, 892, 1699, 1841, 2016
BstYI RGATCY 7 cut(s) 89, 267, 744, 892, 1699, 1841, 2016
BsuRI GGCC 7 cut(s) 28, 43, 64, 475, 1082, 1872, 2317
BtgI CCRYGG 1 cut(s) 2192
BtgZI GCGATG 1 cut(s) 1328
BtsCI GGATG 5 cut(s) 1120, 1762, 1956, 2100, 2314
BtsIMutI CAGTG 3 cut(s) 907, 1162, 2270
Cac8I GCNNGC 6 cut(s) 30, 755, 1792, 1892, 1943, 2279
CfoI GCGC 1 cut(s) 1065
Cfr13I GGNCC 7 cut(s) 26, 27, 41, 965, 1146, 1478, 2267
Csp6I GTAC 4 cut(s) 151, 959, 1224, 1307
CviQI GTAC 4 cut(s) 151, 959, 1224, 1307
DdeI CTNAG 8 cut(s) 171, 1019, 1154, 1467, 1594, 1690, 1748, 1886
DinI GGCGCC 1 cut(s) 1064
DpnI GATC 9 cut(s) 91, 269, 746, 769, 894, 1701, 1843, 2018, 2313
DpnII GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
DraI TTTAAA 1 cut(s) 1974
DraIII CACNNNGTG 1 cut(s) 1034
DriI GACNNNNNGTC 1 cut(s) 2071
EaeI YGGCCR 3 cut(s) 473, 1870, 2315
Eam1105I GACNNNNNGTC 1 cut(s) 2071
EciI GGCGGA 1 cut(s) 1528
Ecl136II GAGCTC 1 cut(s) 2173
Eco130I CCWWGG 1 cut(s) 774
Eco24I GRGCYC 3 cut(s) 30, 2034, 2175
Eco32I GATATC 2 cut(s) 1294, 1740
Eco47I GGWCC 4 cut(s) 965, 1146, 1478, 2267
Eco53kI GAGCTC 1 cut(s) 2173
Eco57I CTGAAG 4 cut(s) 51, 398, 908, 1373
Eco72I CACGTG 2 cut(s) 1139, 1545
Eco88I CYCGRG 1 cut(s) 2153
EcoICRI GAGCTC 1 cut(s) 2173
EcoO109I RGGNCCY 3 cut(s) 26, 41, 965
EcoRI GAATTC 2 cut(s) 1487, 1527
EcoRV GATATC 2 cut(s) 1294, 1740
EcoT14I CCWWGG 1 cut(s) 774
EcoT38I GRGCYC 3 cut(s) 30, 2034, 2175
EgeI GGCGCC 1 cut(s) 1064
EheI GGCGCC 1 cut(s) 1064
ErhI CCWWGG 1 cut(s) 774
FalI AAGNNNNNCTT 6 cut(s) 254, 286, 767, 799, 1673, 1705
FaqI GGGAC 2 cut(s) 502, 849
FauI CCCGC 2 cut(s) 37, 2348
FauNDI CATATG 2 cut(s) 690, 1922
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 4 cut(s) 426, 473, 501, 804
FokI GGATG 5 cut(s) 1107, 1749, 1963, 2107, 2321
FriOI GRGCYC 3 cut(s) 30, 2034, 2175
Fsp4HI GCNGC 4 cut(s) 426, 473, 501, 804
FspBI CTAG 9 cut(s) 141, 398, 506, 582, 605, 635, 851, 1104, 1784
GlaI GCGC 1 cut(s) 1064
GluI GCNGC 4 cut(s) 426, 473, 501, 804
GsuI CTGGAG 2 cut(s) 1260, 2070
HaeII RGCGCY 1 cut(s) 1066
HaeIII GGCC 7 cut(s) 28, 43, 64, 475, 1082, 1872, 2317
HapII CCGG 6 cut(s) 248, 750, 1948, 2079, 2261, 2306
HhaI GCGC 1 cut(s) 1065
Hin1I GRCGYC 1 cut(s) 1063
Hin6I GCGC 1 cut(s) 1063
HinP1I GCGC 1 cut(s) 1063
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 1883
HpaII CCGG 6 cut(s) 248, 750, 1948, 2079, 2261, 2306
HphI GGTGA 9 cut(s) 129, 837, 1172, 1298, 1370, 1431, 1955, 2119, 2170
Hpy166II GTNNAC 5 cut(s) 79, 733, 1037, 1963, 2270
Hpy8I GTNNAC 5 cut(s) 79, 733, 1037, 1963, 2270
HpyAV CCTTC 9 cut(s) 75, 978, 1134, 1443, 1491, 1890, 2056, 2134, 2192
HpyCH4III ACNGT 8 cut(s) 616, 863, 902, 1606, 1988, 2011, 2193, 2267
HpyCH4IV ACGT 4 cut(s) 515, 1138, 1463, 1544
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 34, 311, 500, 562, 1150, 1310, 1891
HpyF3I CTNAG 8 cut(s) 171, 1019, 1154, 1467, 1594, 1690, 1748, 1886
HpySE526I ACGT 4 cut(s) 515, 1138, 1463, 1544
Hsp92I GRCGYC 1 cut(s) 1063
HspAI GCGC 1 cut(s) 1063
KasI GGCGCC 1 cut(s) 1062
Kpn2I TCCGGA 2 cut(s) 247, 2260
Kzo9I GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
LmnI GCTCC 1 cut(s) 2170
Lsp1109I GCAGC 3 cut(s) 412, 487, 815
LweI GCATC 4 cut(s) 10, 188, 1129, 1771
MaeI CTAG 9 cut(s) 141, 398, 506, 582, 605, 635, 851, 1104, 1784
MaeII ACGT 4 cut(s) 515, 1138, 1463, 1544
MaeIII GTNAC 4 cut(s) 759, 1428, 1819, 1916
MalI GATC 9 cut(s) 91, 269, 746, 769, 894, 1701, 1843, 2018, 2313
MboI GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 7 cut(s) 89, 267, 744, 892, 1699, 1841, 2016
MhlI GDGCHC 5 cut(s) 30, 951, 1138, 2034, 2175
MlsI TGGCCA 1 cut(s) 1872
MluCI AATT 9 cut(s) 83, 306, 1473, 1487, 1505, 1527, 1598, 1623, 2256
MluNI TGGCCA 1 cut(s) 1872
Mly113I GGCGCC 1 cut(s) 1063
MlyI GAGTC 5 cut(s) 549, 928, 1465, 1544, 2060
MmeI TCCRAC 4 cut(s) 568, 1588, 1600, 2276
Mox20I TGGCCA 1 cut(s) 1872
MroI TCCGGA 2 cut(s) 247, 2260
MroXI GAANNNNTTC 4 cut(s) 219, 1644, 2109, 2202
MscI TGGCCA 1 cut(s) 1872
MseI TTAA 4 cut(s) 1250, 1514, 1865, 1973
MslI CAYNNNNRTG 3 cut(s) 1767, 1835, 1866
Msp20I TGGCCA 1 cut(s) 1872
MspA1I CMGCKG 2 cut(s) 425, 1096
MspI CCGG 6 cut(s) 248, 750, 1948, 2079, 2261, 2306
MspR9I CCNGG 1 cut(s) 750
MunI CAATTG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 210
MvnI CGCG 1 cut(s) 1968
MwoI GCNNNNNNNGC 8 cut(s) 29, 34, 311, 500, 562, 1150, 1310, 1891
NarI GGCGCC 1 cut(s) 1063
NciI CCSGG 1 cut(s) 750
NdeI CATATG 2 cut(s) 690, 1922
NdeII GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
NlaIV GGNNCC 8 cut(s) 28, 106, 746, 894, 1064, 1843, 2100, 2245
NmuCI GTSAC 1 cut(s) 1916
NspI RCATGY 4 cut(s) 526, 1198, 1794, 1945
NspV TTCGAA 1 cut(s) 2113
PaeI GCATGC 2 cut(s) 1794, 1945
PciI ACATGT 1 cut(s) 522
PctI GAATGC 1 cut(s) 210
PdmI GAANNNNTTC 4 cut(s) 219, 1644, 2109, 2202
PfeI GAWTC 7 cut(s) 134, 220, 677, 818, 833, 842, 1858
PflMI CCANNNNNTGG 2 cut(s) 550, 1283
PkrI GCNGC 4 cut(s) 427, 474, 502, 805
PleI GAGTC 5 cut(s) 548, 927, 1464, 1543, 2060
PluTI GGCGCC 1 cut(s) 1066
PmaCI CACGTG 2 cut(s) 1139, 1545
PmlI CACGTG 2 cut(s) 1139, 1545
PpsI GAGTC 5 cut(s) 548, 927, 1464, 1543, 2060
Ppu21I YACGTR 2 cut(s) 1139, 1545
PpuMI RGGWCCY 1 cut(s) 965
PscI ACATGT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 2175
Psp5II RGGWCCY 1 cut(s) 965
PspCI CACGTG 2 cut(s) 1139, 1545
PspN4I GGNNCC 8 cut(s) 28, 106, 746, 894, 1064, 1843, 2100, 2245
PspOMI GGGCCC 1 cut(s) 26
PspPI GGNCC 7 cut(s) 26, 27, 41, 965, 1146, 1478, 2267
PspPPI RGGWCCY 1 cut(s) 965
PstI CTGCAG 1 cut(s) 805
PsuI RGATCY 7 cut(s) 89, 267, 744, 892, 1699, 1841, 2016
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 4 cut(s) 152, 960, 1225, 1308
RsaNI GTAC 4 cut(s) 151, 959, 1224, 1307
RseI CAYNNNNRTG 3 cut(s) 1767, 1835, 1866
SacI GAGCTC 1 cut(s) 2175
SalI GTCGAC 1 cut(s) 731
SaqAI TTAA 4 cut(s) 1250, 1514, 1865, 1973
SatI GCNGC 4 cut(s) 426, 473, 501, 804
Sau3AI GATC 9 cut(s) 89, 267, 744, 767, 892, 1699, 1841, 2016, 2311
Sau96I GGNCC 7 cut(s) 26, 27, 41, 965, 1146, 1478, 2267
SchI GAGTC 5 cut(s) 549, 928, 1465, 1544, 2060
ScrFI CCNGG 1 cut(s) 750
SduI GDGCHC 5 cut(s) 30, 951, 1138, 2034, 2175
SfaNI GCATC 4 cut(s) 10, 188, 1129, 1771
SfcI CTRYAG 2 cut(s) 801, 1210
SfoI GGCGCC 1 cut(s) 1064
SfuI TTCGAA 1 cut(s) 2113
SinI GGWCC 4 cut(s) 965, 1146, 1478, 2267
SmiMI CAYNNNNRTG 3 cut(s) 1767, 1835, 1866
SmlI CTYRAG 5 cut(s) 121, 260, 1204, 1532, 2035
SmoI CTYRAG 5 cut(s) 121, 260, 1204, 1532, 2035
SpeI ACTAGT 1 cut(s) 397
SphI GCATGC 2 cut(s) 1794, 1945
Sse9I AATT 9 cut(s) 83, 306, 1473, 1487, 1505, 1527, 1598, 1623, 2256
SsiI CCGC 9 cut(s) 30, 472, 742, 824, 1096, 1144, 1539, 1966, 2341
SspDI GGCGCC 1 cut(s) 1062
SspI AATATT 1 cut(s) 1585
SspMI CTAG 9 cut(s) 141, 398, 506, 582, 605, 635, 851, 1104, 1784
SstI GAGCTC 1 cut(s) 2175
StyD4I CCNGG 1 cut(s) 748
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 8 cut(s) 616, 863, 902, 1606, 1988, 2011, 2193, 2267
TaiI ACGT 4 cut(s) 518, 1141, 1466, 1547
TaqI TCGA 7 cut(s) 535, 732, 953, 1072, 1132, 1648, 2113
TasI AATT 9 cut(s) 83, 306, 1473, 1487, 1505, 1527, 1598, 1623, 2256
TatI WGTACW 1 cut(s) 958
TauI GCSGC 1 cut(s) 475
TfiI GAWTC 7 cut(s) 134, 220, 677, 818, 833, 842, 1858
Tru1I TTAA 4 cut(s) 1250, 1514, 1865, 1973
Tru9I TTAA 4 cut(s) 1250, 1514, 1865, 1973
TscAI CASTG 3 cut(s) 907, 1162, 2277
TseFI GTSAC 1 cut(s) 1916
TseI GCWGC 3 cut(s) 425, 500, 803
Tsp45I GTSAC 1 cut(s) 1916
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 3 cut(s) 907, 1162, 2277
Van91I CCANNNNNTGG 2 cut(s) 550, 1283
VpaK11BI GGWCC 4 cut(s) 965, 1146, 1478, 2267
XapI RAATTY 4 cut(s) 83, 1487, 1527, 2256
XceI RCATGY 4 cut(s) 526, 1198, 1794, 1945
XcmI CCANNNNNNNNNTGG 1 cut(s) 54
XmiI GTMKAC 1 cut(s) 732
XmnI GAANNNNTTC 4 cut(s) 219, 1644, 2109, 2202
XspI CTAG 9 cut(s) 141, 398, 506, 582, 605, 635, 851, 1104, 1784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.