Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0016627

Physical Location & Seq

Genomic Coordinates Forward (+)

30827679 .. 30829053

Gene Structure 1375 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1241000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGATGTTAGAGCGTGCTAGCTTAACATTTTTCATGGTGTGCATTTTGTGCATATCATCTACAGCATGTGCCCAAGAGTACAAGCCTCTAGAGGGTGACCCCAAAGCCATGAGGCACAGATATCAAAGGTGGCAGGACAAATATGGCCGAAAATATAAGAGTGAAGAGGAAAAGGCATACCGTTTTGGGATATACAAATCCAACCTTGAGTTTGTTGATTTCATAAATTCTCAAAATCTTTCATACAAACTCAGCGACAACAAGTTTGCAGATATCACAAACATAGAATTCACAACAAACTACATGGGTTTCCAAACTAGGAGAGATCTAAAGATGAGGTTCAGCTATGACAAGGAAGAAGATTTGCCGACTACAGTGGATTGGAGAAAGCGTGGTGCGGTAACTCCAATCAAGGAGCAAGGCAAATGTGGAAGTTGTTGGGCTTTCTCAGCCGTAGCAGCTGTTGAAGGAATTAACAAAATCAAAACTGGAAACTTGGTGTCACTATCAGAGCAAGAACTTGTGGACTGCGATGTCAACTCTGGGAACCAAGGCTGTAGTGGTGGATTCATGGAGAAAGCGTTTAGTTTCATAAAAGATAACGGACTCTCCACCGAAGAAGATTATCCCTACAAAGGATTACAGGGTACCTGTGACGAGGATAAATTGAAAAGACGTGCTGTGAACATAAGTGGCTATGAAAGAATACCTGTCAATAACGAGAAAAGCCTACAAGCCGCAGTTGCTCGCCAACCTGTCTCTGTTGCAATCGATGCTGGCAGTTATGGTTTTCAACTCTATTCTTCAGGTATCTTCACTGGTTATTGTGGAAAGAATCTCAACCATGGAGTCACCGCAGTAGGGTATGGAGAAGATAGTGGTAAAAAGTACTGGATTGTGAAGAATTCCTGGGGTCTTGACTGGGGTGAATCTGGTTATGTAAGAATAACACGCAACTCGGCTGATAAGGAAGGTACTTGCGGCATTGCCATGCAGGCTAGCTACCCTGTTCAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

340

Amino Acids

38.11

Weight (kDa)

7.92

Isoelectric Point (pI)

33.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	41 - 97	2.9e-16	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	122 - 337	8.8e-84	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016627)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06260
fragaria_vesca	FvH4_7g08420
malus_domestica	MD09G1241000.v1.1
prunus_persica	Prupe.3G125700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c20g0500241
rosa_laevigata	RLG00000028996
rosa_multiflora	Rmu_sc0010800.1_g000009
rosa_roxburghii	Rroxscaffold_4G00311040
rosa_rugosa	Rorug01G0165300 Rorug06G0126100
rosa_samantha	Rh1AG180400 Rh1BG148600 Rh1CG167300
rosa_wichuraiana	Rw1G014990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	533
Acc65I	GGTACC	1 cut(s)	647
AccB1I	GGYRCC	1 cut(s)	647
AciI	CCGC	4 cut(s)	398, 738, 855, 981
AcoI	YGGCCR	1 cut(s)	145
AcsI	RAATTY	3 cut(s)	226, 287, 904
AcuI	CTGAAG	1 cut(s)	789
AfaI	GTAC	4 cut(s)	80, 649, 890, 976
AfiI	CCNNNNNNNGG	4 cut(s)	92, 635, 861, 1012
AgsI	TTSAA	3 cut(s)	467, 670, 794
AjiI	CACGTC	1 cut(s)	677
AjnI	CCWGG	1 cut(s)	908
AluBI	AGCT	4 cut(s)	21, 345, 461, 1002
AluI	AGCT	4 cut(s)	21, 345, 461, 1002
Alw26I	GTCTC	1 cut(s)	763
AoxI	GGCC	1 cut(s)	145
ApeKI	GCWGC	1 cut(s)	458
ApoI	RAATTY	3 cut(s)	226, 287, 904
ArsI	GACNNNNNNTTYG	2 cut(s)	248, 280
Asp718I	GGTACC	1 cut(s)	647
AsuHPI	GGTGA	3 cut(s)	107, 844, 938
AsuNHI	GCTAGC	2 cut(s)	17, 998
BaeGI	GKGCMC	1 cut(s)	73
BanI	GGYRCC	1 cut(s)	647
BbvI	GCAGC	1 cut(s)	470
BceAI	ACGGC	1 cut(s)	437
BciT130I	CCWGG	1 cut(s)	910
BcoDI	GTCTC	1 cut(s)	763
BfaI	CTAG	4 cut(s)	18, 89, 318, 999
BfmI	CTRYAG	3 cut(s)	60, 372, 556
BglI	GCCNNNNNGGC	1 cut(s)	995
BglII	AGATCT	1 cut(s)	325
BisI	GCNGC	3 cut(s)	459, 738, 982
BlsI	GCNGC	3 cut(s)	460, 739, 983
BmcAI	AGTACT	1 cut(s)	890
Bme1390I	CCNGG	1 cut(s)	910
BmgBI	CACGTC	1 cut(s)	677
BmiI	GGNNCC	2 cut(s)	548, 649
BmrFI	CCNGG	1 cut(s)	910
BmrI	ACTGGG	1 cut(s)	931
BmsI	GCATC	1 cut(s)	763
BmtI	GCTAGC	2 cut(s)	21, 1002
BmuI	ACTGGG	1 cut(s)	931
BpuEI	CTTGAG	1 cut(s)	227
Bsa29I	ATCGAT	1 cut(s)	771
BsaJI	CCNNGG	3 cut(s)	550, 844, 909
BsaXI	ACNNNNNCTCC	6 cut(s)	376, 406, 566, 593, 596, 623
Bsc4I	CCNNNNNNNGG	4 cut(s)	92, 635, 861, 1012
Bse1I	ACTGG	4 cut(s)	493, 823, 896, 926
Bse3DI	GCAATG	1 cut(s)	984
BseBI	CCWGG	1 cut(s)	910
BseCI	ATCGAT	1 cut(s)	771
BseDI	CCNNGG	3 cut(s)	550, 844, 909
BseLI	CCNNNNNNNGG	4 cut(s)	92, 635, 861, 1012
BseMI	GCAATG	1 cut(s)	984
BseMII	CTCAG	2 cut(s)	265, 462
BseNI	ACTGG	4 cut(s)	493, 823, 896, 926
BseSI	GKGCMC	1 cut(s)	73
BseXI	GCAGC	1 cut(s)	470
BshFI	GGCC	1 cut(s)	147
BshNI	GGYRCC	1 cut(s)	647
BshVI	ATCGAT	1 cut(s)	771
BslI	CCNNNNNNNGG	4 cut(s)	92, 635, 861, 1012
BsmAI	GTCTC	1 cut(s)	763
BsnI	GGCC	1 cut(s)	147
Bsp1286I	GDGCHC	1 cut(s)	73
Bsp143I	GATC	1 cut(s)	325
Bsp19I	CCATGG	1 cut(s)	844
BspACI	CCGC	4 cut(s)	398, 738, 855, 981
BspANI	GGCC	1 cut(s)	147
BspCNI	CTCAG	2 cut(s)	264, 461
BspDI	ATCGAT	1 cut(s)	771
BspLI	GGNNCC	2 cut(s)	548, 649
BspOI	GCTAGC	2 cut(s)	21, 1002
BspT107I	GGYRCC	1 cut(s)	647
BsrDI	GCAATG	1 cut(s)	984
BsrI	ACTGG	4 cut(s)	493, 823, 896, 926
BssECI	CCNNGG	3 cut(s)	550, 844, 909
BssMI	GATC	1 cut(s)	325
BssT1I	CCWWGG	2 cut(s)	550, 844
Bst2UI	CCWGG	1 cut(s)	910
Bst4CI	ACNGT	2 cut(s)	182, 376
Bst6I	CTCTTC	1 cut(s)	159
BstAPI	GCANNNNNTGC	2 cut(s)	48, 773
BstC8I	GCNNGC	6 cut(s)	15, 19, 748, 778, 996, 1000
BstDEI	CTNAG	2 cut(s)	251, 448
BstDSI	CCRYGG	1 cut(s)	844
BstEII	GGTNACC	1 cut(s)	95
BstKTI	GATC	1 cut(s)	328
BstMAI	GTCTC	1 cut(s)	763
BstMBI	GATC	1 cut(s)	325
BstMWI	GCNNNNNNNGC	6 cut(s)	48, 449, 458, 743, 773, 995
BstNI	CCWGG	1 cut(s)	910
BstNSI	RCATGY	1 cut(s)	69
BstPI	GGTNACC	1 cut(s)	95
BstSCI	CCNGG	1 cut(s)	908
BstSFI	CTRYAG	3 cut(s)	60, 372, 556
BstSLI	GKGCMC	1 cut(s)	73
BstV1I	GCAGC	1 cut(s)	470
BstX2I	RGATCY	1 cut(s)	325
BstYI	RGATCY	1 cut(s)	325
Bsu15I	ATCGAT	1 cut(s)	771
BsuRI	GGCC	1 cut(s)	147
BsuTUI	ATCGAT	1 cut(s)	771
BtgI	CCRYGG	1 cut(s)	844
BtgZI	GCGATG	1 cut(s)	546
BtrI	CACGTC	1 cut(s)	677
BtsIMutI	CAGTG	2 cut(s)	381, 816
Cac8I	GCNNGC	6 cut(s)	15, 19, 748, 778, 996, 1000
ClaI	ATCGAT	1 cut(s)	771
Csp6I	GTAC	4 cut(s)	79, 648, 889, 975
CviAII	CATG	7 cut(s)	34, 66, 109, 304, 571, 845, 991
CviQI	GTAC	4 cut(s)	79, 648, 889, 975
DdeI	CTNAG	2 cut(s)	251, 448
DpnI	GATC	1 cut(s)	327
DpnII	GATC	1 cut(s)	325
DrdI	GACNNNNNNGTC	1 cut(s)	533
DseDI	GACNNNNNNGTC	1 cut(s)	533
EaeI	YGGCCR	1 cut(s)	145
Eam1104I	CTCTTC	1 cut(s)	159
EarI	CTCTTC	1 cut(s)	159
Eco130I	CCWWGG	2 cut(s)	550, 844
Eco32I	GATATC	2 cut(s)	122, 274
Eco57I	CTGAAG	1 cut(s)	789
Eco91I	GGTNACC	1 cut(s)	95
EcoO65I	GGTNACC	1 cut(s)	95
EcoRI	GAATTC	2 cut(s)	287, 904
EcoRII	CCWGG	1 cut(s)	908
EcoRV	GATATC	2 cut(s)	122, 274
EcoT14I	CCWWGG	2 cut(s)	550, 844
ErhI	CCWWGG	2 cut(s)	550, 844
FaeI	CATG	7 cut(s)	37, 69, 112, 307, 574, 848, 994
FatI	CATG	7 cut(s)	33, 65, 108, 303, 570, 844, 990
Fnu4HI	GCNGC	3 cut(s)	459, 738, 982
Fsp4HI	GCNGC	3 cut(s)	459, 738, 982
FspBI	CTAG	4 cut(s)	18, 89, 318, 999
GluI	GCNGC	3 cut(s)	459, 738, 982
HaeIII	GGCC	1 cut(s)	147
Hin1II	CATG	7 cut(s)	37, 69, 112, 307, 574, 848, 994
HincII	GTYRAC	1 cut(s)	538
HindII	GTYRAC	1 cut(s)	538
HinfI	GANTC	5 cut(s)	567, 606, 835, 849, 929
HphI	GGTGA	3 cut(s)	107, 844, 938
Hpy166II	GTNNAC	3 cut(s)	526, 538, 685
Hpy188I	TCNGA	1 cut(s)	511
Hpy188III	TCNNGA	2 cut(s)	89, 917
Hpy8I	GTNNAC	3 cut(s)	526, 538, 685
HpyAV	CCTTC	2 cut(s)	461, 965
HpyCH4III	ACNGT	2 cut(s)	182, 376
HpyCH4IV	ACGT	1 cut(s)	676
HpyCH4V	TGCA	5 cut(s)	42, 51, 269, 767, 994
HpyF10VI	GCNNNNNNNGC	6 cut(s)	48, 449, 458, 743, 773, 995
HpyF3I	CTNAG	2 cut(s)	251, 448
HpySE526I	ACGT	1 cut(s)	676
Hsp92II	CATG	7 cut(s)	37, 69, 112, 307, 574, 848, 994
KpnI	GGTACC	1 cut(s)	651
Kzo9I	GATC	1 cut(s)	325
LmnI	GCTCC	1 cut(s)	415
Lsp1109I	GCAGC	1 cut(s)	470
LweI	GCATC	1 cut(s)	763
MaeI	CTAG	4 cut(s)	18, 89, 318, 999
MaeII	ACGT	1 cut(s)	676
MaeIII	GTNAC	5 cut(s)	95, 400, 501, 653, 850
MalI	GATC	1 cut(s)	327
MboI	GATC	1 cut(s)	325
MboII	GAAGA	9 cut(s)	176, 368, 371, 629, 632, 795, 805, 884, 913
MflI	RGATCY	1 cut(s)	325
MhlI	GDGCHC	1 cut(s)	73
MluCI	AATT	5 cut(s)	226, 287, 471, 665, 904
MlyI	GAGTC	2 cut(s)	600, 858
MmeI	TCCRAC	1 cut(s)	225
MnlI	CCTC	6 cut(s)	85, 96, 105, 160, 330, 652
MseI	TTAA	2 cut(s)	23, 474
MslI	CAYNNNNRTG	1 cut(s)	989
MspA1I	CMGCKG	1 cut(s)	461
MspR9I	CCNGG	1 cut(s)	910
MvaI	CCWGG	1 cut(s)	910
MwoI	GCNNNNNNNGC	6 cut(s)	48, 449, 458, 743, 773, 995
NcoI	CCATGG	1 cut(s)	844
NdeII	GATC	1 cut(s)	325
NheI	GCTAGC	2 cut(s)	17, 998
NlaIII	CATG	7 cut(s)	37, 69, 112, 307, 574, 848, 994
NlaIV	GGNNCC	2 cut(s)	548, 649
NmeAIII	GCCGAG	1 cut(s)	938
NmuCI	GTSAC	4 cut(s)	95, 501, 653, 850
NspI	RCATGY	1 cut(s)	69
PfeI	GAWTC	3 cut(s)	567, 835, 929
PkrI	GCNGC	3 cut(s)	460, 739, 983
PleI	GAGTC	2 cut(s)	600, 857
PpsI	GAGTC	2 cut(s)	600, 857
Psp6I	CCWGG	1 cut(s)	908
PspEI	GGTNACC	1 cut(s)	95
PspGI	CCWGG	1 cut(s)	908
PspN4I	GGNNCC	2 cut(s)	548, 649
PsuI	RGATCY	1 cut(s)	325
PvuII	CAGCTG	1 cut(s)	461
RsaI	GTAC	4 cut(s)	80, 649, 890, 976
RsaNI	GTAC	4 cut(s)	79, 648, 889, 975
RseI	CAYNNNNRTG	1 cut(s)	989
SaqAI	TTAA	2 cut(s)	23, 474
SatI	GCNGC	3 cut(s)	459, 738, 982
Sau3AI	GATC	1 cut(s)	325
ScaI	AGTACT	1 cut(s)	890
SchI	GAGTC	2 cut(s)	600, 858
ScrFI	CCNGG	1 cut(s)	910
SduI	GDGCHC	1 cut(s)	73
SfaNI	GCATC	1 cut(s)	763
SfcI	CTRYAG	3 cut(s)	60, 372, 556
SmiMI	CAYNNNNRTG	1 cut(s)	989
SmlI	CTYRAG	1 cut(s)	206
SmoI	CTYRAG	1 cut(s)	206
Sse9I	AATT	5 cut(s)	226, 287, 471, 665, 904
SsiI	CCGC	4 cut(s)	398, 738, 855, 981
SspMI	CTAG	4 cut(s)	18, 89, 318, 999
StyD4I	CCNGG	1 cut(s)	908
StyI	CCWWGG	2 cut(s)	550, 844
TaaI	ACNGT	2 cut(s)	182, 376
TaiI	ACGT	1 cut(s)	679
TaqI	TCGA	1 cut(s)	771
TasI	AATT	5 cut(s)	226, 287, 471, 665, 904
TatI	WGTACW	2 cut(s)	78, 888
TauI	GCSGC	2 cut(s)	740, 984
TfiI	GAWTC	3 cut(s)	567, 835, 929
Tru1I	TTAA	2 cut(s)	23, 474
Tru9I	TTAA	2 cut(s)	23, 474
TscAI	CASTG	2 cut(s)	381, 823
TseFI	GTSAC	4 cut(s)	95, 501, 653, 850
TseI	GCWGC	1 cut(s)	458
Tsp45I	GTSAC	4 cut(s)	95, 501, 653, 850
TspDTI	ATGAA	6 cut(s)	22, 211, 231, 559, 580, 714
TspGWI	ACGGA	1 cut(s)	618
TspRI	CASTG	2 cut(s)	381, 823
XapI	RAATTY	3 cut(s)	226, 287, 904
XbaI	TCTAGA	1 cut(s)	88
XceI	RCATGY	1 cut(s)	69
XcmI	CCANNNNNNNNNTGG	1 cut(s)	557
XspI	CTAG	4 cut(s)	18, 89, 318, 999
ZrmI	AGTACT	1 cut(s)	890

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.