MD09G1248000.v1.1

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015837
Physical Location & Seq
Reverse (-)
31792699 .. 31794505
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1248000.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGCGTTTTCATTTCATCCCTTTTTCTTCTTCTTGTTCCTTGTATTGGGTTTTGCCTCCTCCACCACCTTCCTCTCTTATGATATCTTCGAGTCAGGTGGATCATCCACCGGAGGACGTGCCCTTCTTCAGGCCAAAAAAAATTGTCCTGTGAACTTTGAGAATCAGAACTACACAATCATTACAAGCAAGTGCAAAGGACCAAACTACCCAGCAAAGAGCTGCTGTGATGCTTTGAAGGATTTTGCTTGTCCTTTTACAGAAGAAATCAATGACTTGAAAAATGATTGTGCTTCAACCATGTTCAGCTACATAAACATCTACGGGAAGTACCCTCCCGGTCTGTTTGCGTCTCAATGCCGCGAAGGGAAAGAAGGTCTCGCATGTCCCGCTGAGGCACCGAAATCTGATCAGAAGAGCAGCAGAGGTCACATTTCAGCTACTCACTCCATCATGCTAGCACTAACTGCTGCCTTGGTTGTATTACCATCCTTCCATAGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.42

Weight (kDa)

8.1

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LLG1 PF26578 51 - 127 1.9e-40 LLG1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20700
fragaria_vesca FvH4_3g38900
malus_domestica MD09G1248000.v1.1 MD17G1240900.v1.1
prunus_persica Prupe.3G137300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0070141
rosa_laevigata RLG00000036115
rosa_multiflora Rmu_sc0002109.1_g000010
rosa_roxburghii Rroxscaffold_1G00010980
rosa_rugosa Rorug05G0403000
rosa_samantha Rh5AG459100 Rh5BG477700 Rh5CG501700 Rh5DG489700
rosa_wichuraiana Rw5G042830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AccII CGCG 1 cut(s) 363
AciI CCGC 2 cut(s) 361, 390
AclWI GGATC 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 46, 130, 498
AgsI TTSAA 3 cut(s) 238, 280, 297
AjiI CACGTC 1 cut(s) 119
AluBI AGCT 3 cut(s) 222, 309, 440
AluI AGCT 3 cut(s) 222, 309, 440
Alw26I GTCTC 2 cut(s) 357, 383
AlwI GGATC 1 cut(s) 109
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 222, 420, 470
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 339
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 397
BbvCI CCTCAGC 1 cut(s) 393
BbvI GCAGC 3 cut(s) 209, 432, 457
BccI CCATC 2 cut(s) 458, 496
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 357, 383
BfaI CTAG 1 cut(s) 458
BisI GCNGC 4 cut(s) 223, 361, 421, 471
BlsI GCNGC 4 cut(s) 224, 362, 422, 472
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 1 cut(s) 220
BmtI GCTAGC 1 cut(s) 461
Bpu10I CCTNAGC 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 130, 498
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 3 cut(s) 16, 104, 488
BseLI CCNNNNNNNGG 3 cut(s) 46, 130, 498
BseMII CTCAG 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 3 cut(s) 209, 432, 457
BseYI CCCAGC 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 134
BshNI GGYRCC 1 cut(s) 397
BsiSI CCGG 2 cut(s) 111, 339
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 3 cut(s) 46, 130, 498
BsmAI GTCTC 2 cut(s) 357, 383
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 372
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 2 cut(s) 101, 409
BspACI CCGC 2 cut(s) 361, 390
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 363
BspLI GGNNCC 1 cut(s) 399
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 2 cut(s) 101, 409
BssT1I CCWWGG 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 1 cut(s) 393
BstENI CCTNNNNNAGG 2 cut(s) 128, 496
BstF5I GGATG 3 cut(s) 16, 104, 488
BstFNI CGCG 1 cut(s) 363
BstKTI GATC 2 cut(s) 104, 412
BstMAI GTCTC 2 cut(s) 357, 383
BstMBI GATC 2 cut(s) 101, 409
BstMWI GCNNNNNNNGC 2 cut(s) 389, 467
BstNSI RCATGY 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 124
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 3 cut(s) 209, 432, 457
BsuRI GGCC 1 cut(s) 134
BtrI CACGTC 1 cut(s) 119
BtsCI GGATG 3 cut(s) 16, 104, 488
Cac8I GCNNGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 200
CseI GACGC 1 cut(s) 339
Csp6I GTAC 1 cut(s) 331
CviAII CATG 3 cut(s) 301, 384, 454
CviJI RGCY 4 cut(s) 134, 222, 309, 440
CviKI_1 RGCY 4 cut(s) 134, 222, 309, 440
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 1 cut(s) 393
DpnI GATC 2 cut(s) 103, 411
DpnII GATC 2 cut(s) 101, 409
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 383
Eco32I GATATC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 113
EcoNI CCTNNNNNAGG 2 cut(s) 128, 496
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 3 cut(s) 304, 387, 457
FaiI YATR 6 cut(s) 81, 302, 314, 385, 455, 498
FaqI GGGAC 1 cut(s) 372
FatI CATG 3 cut(s) 300, 383, 453
FauI CCCGC 1 cut(s) 397
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 223, 361, 421, 471
FokI GGATG 3 cut(s) 3, 91, 475
Fsp4HI GCNGC 4 cut(s) 223, 361, 421, 471
FspBI CTAG 1 cut(s) 458
GluI GCNGC 4 cut(s) 223, 361, 421, 471
GsaI CCCAGC 1 cut(s) 215
HaeIII GGCC 1 cut(s) 134
HapII CCGG 2 cut(s) 111, 339
HgaI GACGC 1 cut(s) 339
Hin1II CATG 3 cut(s) 304, 387, 457
HinfI GANTC 2 cut(s) 92, 163
HpaII CCGG 2 cut(s) 111, 339
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 4 cut(s) 168, 409, 414, 506
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 6 cut(s) 79, 134, 232, 359, 368, 502
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 467
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 3 cut(s) 304, 387, 457
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 2 cut(s) 101, 409
LguI GCTCTTC 1 cut(s) 410
LpnPI CCDG 6 cut(s) 81, 116, 124, 162, 225, 352
Lsp1109I GCAGC 3 cut(s) 209, 432, 457
LweI GCATC 1 cut(s) 220
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 103, 411
MboI GATC 2 cut(s) 101, 409
MboII GAAGA 6 cut(s) 19, 22, 79, 119, 275, 427
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 1 cut(s) 142
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 7 cut(s) 67, 70, 83, 107, 345, 388, 419
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 2 cut(s) 111, 339
MspR9I CCNGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 389, 467
NciI CCSGG 1 cut(s) 339
NdeII GATC 2 cut(s) 101, 409
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 3 cut(s) 304, 387, 457
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 1 cut(s) 387
PciSI GCTCTTC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 4 cut(s) 224, 362, 422, 472
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 1 cut(s) 200
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SapI GCTCTTC 1 cut(s) 410
SatI GCNGC 4 cut(s) 223, 361, 421, 471
Sau3AI GATC 2 cut(s) 101, 409
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 124
SetI ASST 9 cut(s) 71, 100, 121, 224, 311, 379, 430, 442, 503
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 1 cut(s) 200
Sse9I AATT 1 cut(s) 142
SsiI CCGC 2 cut(s) 361, 390
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 474
TaiI ACGT 1 cut(s) 121
TaqI TCGA 1 cut(s) 90
TasI AATT 1 cut(s) 142
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 1 cut(s) 163
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 3 cut(s) 222, 420, 470
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 5
VpaK11BI GGWCC 1 cut(s) 200
XagI CCTNNNNNAGG 2 cut(s) 128, 496
XceI RCATGY 1 cut(s) 387
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.