Prupe.3G137300_v2.0.a1

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015837
Physical Location & Seq
Forward (+)
14497659 .. 14499806
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G137300.1

Sequence Viewer

Length: 498 bp
ATGTCGTCTGCTTCGAATCACGGTCTTCATCTCTTCTTCTTCTTCTTCCTCTTCTTGGGCTTCGCCTCCTGCACCACCTTCCTTTCCAATGATATTTTCCAGCCTGGTGGATCCATTGGACGCTCCCTTCTTCAGGCCAAACAAAATTGTCCTGTGAACTTTGAGAATCAGAACTACACAATCATCACAAGCAAGTGCAAAGGACCCAACTACCCAGCCAAGAGCTGCTGCGACGCCTTGAAGGACTTTGCCTGCCCTTTTGCAGATGCTATTAATGACTTGAAAAATGATTGTGCTACAACCATGTTCAGCTACATAAACATTTATGGGAAGTACCCTCCTGGCCTGTTTGCGTCTCAATGTCGCGAAGGGAAAGAAGGTCTCGCATGCCCCGCTGAGGCACCGAAACCTCAGAAGAGTAGAGGTCAGATAGCAGCTACTCACTCCATCATACTAGCACTCACTGCAGTCCTAGTATTATCATTCAATATGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

17.99

Weight (kDa)

8.37

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20700
fragaria_vesca FvH4_3g38900
malus_domestica MD09G1248000.v1.1 MD17G1240900.v1.1
prunus_persica Prupe.3G137300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0070141
rosa_laevigata RLG00000036115
rosa_multiflora Rmu_sc0002109.1_g000010
rosa_roxburghii Rroxscaffold_1G00010980
rosa_rugosa Rorug05G0403000
rosa_samantha Rh5AG459100 Rh5BG477700 Rh5CG501700 Rh5DG489700
rosa_wichuraiana Rw5G042830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 400
AccII CGCG 1 cut(s) 366
AciI CCGC 1 cut(s) 393
AclWI GGATC 2 cut(s) 105, 118
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 3 cut(s) 55, 133, 397
AgsI TTSAA 3 cut(s) 241, 283, 487
AjnI CCWGG 2 cut(s) 103, 340
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 3 cut(s) 225, 312, 437
AluI AGCT 3 cut(s) 225, 312, 437
Alw26I GTCTC 2 cut(s) 360, 386
AlwI GGATC 2 cut(s) 105, 118
AoxI GGCC 2 cut(s) 135, 343
ApeKI GCWGC 3 cut(s) 225, 228, 434
AseI ATTAAT 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 203
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 1 cut(s) 203
BamHI GGATCC 1 cut(s) 110
BanI GGYRCC 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 3 cut(s) 212, 215, 446
BccI CCATC 1 cut(s) 455
BciT130I CCWGG 2 cut(s) 105, 342
BcoDI GTCTC 2 cut(s) 360, 386
BfaI CTAG 2 cut(s) 455, 473
BfmI CTRYAG 1 cut(s) 465
BisI GCNGC 3 cut(s) 226, 229, 435
BlsI GCNGC 3 cut(s) 227, 230, 436
Bme1390I CCNGG 2 cut(s) 105, 342
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 3 cut(s) 112, 205, 402
BmrFI CCNGG 2 cut(s) 105, 342
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 386
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 133, 397
BseBI CCWGG 2 cut(s) 105, 342
BseLI CCNNNNNNNGG 3 cut(s) 55, 133, 397
BseMII CTCAG 2 cut(s) 387, 425
BseXI GCAGC 3 cut(s) 212, 215, 446
BseYI CCCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 137, 345
BshNI GGYRCC 1 cut(s) 400
BslI CCNNNNNNNGG 3 cut(s) 55, 133, 397
BsmAI GTCTC 2 cut(s) 360, 386
BsmBI CGTCTC 1 cut(s) 360
BsnI GGCC 2 cut(s) 137, 345
Bso31I GGTCTC 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 14
Bsp143I GATC 1 cut(s) 110
Bsp68I TCGCGA 1 cut(s) 366
BspACI CCGC 1 cut(s) 393
BspANI GGCC 2 cut(s) 137, 345
BspCNI CTCAG 2 cut(s) 388, 424
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 3 cut(s) 112, 205, 402
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 2 cut(s) 105, 118
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 386
BssMI GATC 1 cut(s) 110
BssNI GRCGYC 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 105, 342
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 3 cut(s) 38, 56, 410
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 253, 388
BstDEI CTNAG 2 cut(s) 396, 411
BstENI CCTNNNNNAGG 1 cut(s) 131
BstFNI CGCG 1 cut(s) 366
BstKTI GATC 1 cut(s) 113
BstMAI GTCTC 2 cut(s) 360, 386
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 2 cut(s) 392, 464
BstNI CCWGG 2 cut(s) 105, 342
BstNSI RCATGY 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 103, 340
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 3 cut(s) 212, 215, 446
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 110
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 110
BsuRI GGCC 2 cut(s) 137, 345
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 462
BtuMI TCGCGA 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 253, 388
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 3 cut(s) 129, 242, 342
Csp6I GTAC 1 cut(s) 334
CviAII CATG 2 cut(s) 304, 387
CviJI RGCY 8 cut(s) 60, 103, 137, 218, 225, 312, 345, 437
CviKI_1 RGCY 8 cut(s) 60, 103, 137, 218, 225, 312, 345, 437
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 396, 411
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
Eam1104I CTCTTC 3 cut(s) 38, 56, 410
EarI CTCTTC 3 cut(s) 38, 56, 410
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 103, 340
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 2 cut(s) 307, 390
FaiI YATR 6 cut(s) 305, 317, 327, 388, 452, 491
FatI CATG 2 cut(s) 303, 386
FauI CCCGC 1 cut(s) 400
Fnu4HI GCNGC 3 cut(s) 226, 229, 435
Fsp4HI GCNGC 3 cut(s) 226, 229, 435
FspBI CTAG 2 cut(s) 455, 473
GluI GCNGC 3 cut(s) 226, 229, 435
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 2 cut(s) 137, 345
HgaI GACGC 3 cut(s) 129, 242, 342
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 2 cut(s) 307, 390
HinfI GANTC 2 cut(s) 16, 166
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 4 cut(s) 171, 414, 429, 497
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 5 cut(s) 88, 137, 235, 362, 371
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4V TGCA 4 cut(s) 72, 198, 263, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 464
HpyF3I CTNAG 2 cut(s) 396, 411
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 2 cut(s) 307, 390
Kzo9I GATC 1 cut(s) 110
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 3 cut(s) 212, 215, 446
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 455, 473
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MboII GAAGA 9 cut(s) 17, 25, 28, 31, 34, 37, 43, 122, 427
MflI RGATCY 1 cut(s) 110
MluCI AATT 1 cut(s) 145
MnlI CCTC 6 cut(s) 59, 76, 348, 391, 416, 420
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 105, 342
MvaI CCWGG 2 cut(s) 105, 342
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 2 cut(s) 392, 464
NdeII GATC 1 cut(s) 110
NlaIII CATG 2 cut(s) 307, 390
NlaIV GGNNCC 3 cut(s) 112, 205, 402
NruI TCGCGA 1 cut(s) 366
NspI RCATGY 1 cut(s) 390
NspV TTCGAA 1 cut(s) 14
PaeI GCATGC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PfeI GAWTC 2 cut(s) 16, 166
PkrI GCNGC 3 cut(s) 227, 230, 436
PpuMI RGGWCCY 1 cut(s) 203
PshBI ATTAAT 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 103, 340
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 2 cut(s) 103, 340
PspN4I GGNNCC 3 cut(s) 112, 205, 402
PspPI GGNCC 1 cut(s) 203
PspPPI RGGWCCY 1 cut(s) 203
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 110
RruI TCGCGA 1 cut(s) 366
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 3 cut(s) 226, 229, 435
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 105, 342
SetI ASST 7 cut(s) 80, 227, 314, 382, 412, 427, 439
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 1 cut(s) 203
SphI GCATGC 1 cut(s) 390
Sse9I AATT 1 cut(s) 145
SsiI CCGC 1 cut(s) 393
SspMI CTAG 2 cut(s) 455, 473
StyD4I CCNGG 2 cut(s) 103, 340
TaaI ACNGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 14
TasI AATT 1 cut(s) 145
TfiI GAWTC 2 cut(s) 16, 166
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 469
TseI GCWGC 3 cut(s) 225, 228, 434
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 203
VspI ATTAAT 1 cut(s) 273
XagI CCTNNNNNAGG 1 cut(s) 131
XceI RCATGY 1 cut(s) 390
XspI CTAG 2 cut(s) 455, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.