MD09G1248900.v1.1

Pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0018494
Physical Location & Seq
Reverse (-)
31829417 .. 31832125
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1248900.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGAAGCTTAATTGCATGAGCAAGCTCATCTCCTTCTCCCTTCAAAACCCTCGAAAGAATTACGCTCTTTTCAATTCAATCCGCCCTTCTTCTTCTTCGTCTTCTTCTTCGCCTTCGTGGTCGAATTCTCTCCATGACCGCATAAAGGTCATCAGAGACCCAAAAGCCTCAGTTCTGCCGGTGCTGGAACAATGGGTCAGTGAGGGTCAGGCTGTTGAGAAGCAGCAGCTTCAGTCGCTCGTTCGGCTCTTGAAGGACTTTAGACGCTTCAATCACGCTCTTGAGATATCCCAATGGATGACGGATCGTAGGTATTTTGATTTATCTCCAAGTGATGCTGCTGCTAGATTGAACTTAATCCACAGAGTTCATGGACTAGAGCATGCAGAAAATTACTTCAACAATCTGTCAAAAAGTTTAAAATCGCTTAACGCATATGGCGCCCTTCTATGCATTTATGTGCAAGAGCGGTCTGTTGAGAAAGCAGAAGCGACCATGCAGAAGATGAAGAAGATGGGTATGGCAAAAACCTCATTCCCTTACAACATGTTGATCAATCTTTACTCCCAAAATGGACAATATGAGAAGATCAACATACTGATGCAAGAAATGGAAGAGAATGGAATTCCAATTGACAAGTACACGTTGAGAAATCGAATGATGGCATATATTGCAGCATCTGACATGCCTGGGATGGAAGCTATCCTAAATAGGATGGAGGAAGATCCAAACTTAATCGTCGACTGGAAAATATATTCAATGGCAGCAAATGGGTATCTAAAAGTCGGGTTAACAGAGAAGGCTATTTCAATGCTGAAGATGCTGGAGGGATTGATGCCTCTTCAAGGGAAGAAATCAGTAGAATTTCTTCTGACTCTTTATGCAAGTACTGGCAATAAAGAGGAGCTCTACAGAGTTTGGGATACGTACAAGCCATCGAATGAACCAGTGGATGTACCATATGGCTGCATGATCTCATCTCTGGCGAAGTTGGATGATATTGAGGGGGCTGAAGGGATATTTGAGGAATGGGAGTCACAATGCAAAATATACGACTTTCGGGTGCTGAACAGGCTTCTTGTTGCCTATTGCAAAAGGGGTCTTTTTGATAAGGCAGAATCAGTTGTAAACAAGGCAGTAGAAGGAAGAATCCCTTATGCCAGCACATGGAATGTGTTAGCAATAGGTTATACAGAGAAGCAGCAAATGCCTAAAGCAGTTGAAATGTTGAAGAAGGCACTCTCAGTGGGTAGGCGAGGCTGGGTGCCGCATTCCCCAACTTTGACGGCCTGTTTGGATTATTTGGAAGGGCAGGGAGATATTGAAGGAATTGAAGAGATCATAAGTTTACTAAAAAACTTGGGTCCTTTGTCCGAGGATTTGTATCACAGACTTCTGAGGGCTAGTGTTGCAGCTGGAAAATCAGTTGCTATAATTCTTGATCAGATGAAAGTGGATGGGTTTACTGCTGATGAAGAACCATATAAGGTCATTGAAACAAGCCTAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

504

Amino Acids

57.09

Weight (kDa)

7.05

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 180 - 215 1.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018494)

Species Orthologous Gene IDs
malus_domestica MD09G1248900.v1.1
prunus_persica Prupe.3G136700_v2.0.a1
pyrus_communis pycom09g16580
rosa_chinensis RchiOBHm_Chr2g0126311
rosa_laevigata RLG00000018900
rosa_multiflora Rmu_ssc0000264.1_g000039
rosa_roxburghii Rroxscaffold_2G00117250
rosa_samantha Rh2AG319400 Rh2BG327800 Rh2DG347500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 440, 1264
AccB7I CCANNNNNTGG 1 cut(s) 1167
AccBSI CCGCTC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 741
AciI CCGC 4 cut(s) 82, 139, 469, 1268
AclWI GGATC 2 cut(s) 312, 719
AcsI RAATTY 3 cut(s) 124, 624, 863
AcuI CTGAAG 3 cut(s) 215, 836, 1032
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 4 cut(s) 641, 889, 929, 957
AfiI CCNNNNNNNGG 3 cut(s) 145, 845, 1167
AflIII ACRYGT 2 cut(s) 546, 642
AjnI CCWGG 1 cut(s) 688
AluBI AGCT 6 cut(s) 7, 25, 229, 701, 907, 1415
AluI AGCT 6 cut(s) 7, 25, 229, 701, 907, 1415
Alw21I GWGCWC 1 cut(s) 909
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 2 cut(s) 312, 719
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 1 cut(s) 1287
ApeKI GCWGC 9 cut(s) 223, 226, 338, 341, 674, 764, 966, 1201, 1412
ApoI RAATTY 3 cut(s) 124, 624, 863
ArsI GACNNNNNNTTYG 2 cut(s) 1276, 1308
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 1 cut(s) 1364
AvaII GGWCC 1 cut(s) 1364
BanI GGYRCC 2 cut(s) 440, 1264
BanII GRGCYC 1 cut(s) 909
BarI GAAGNNNNNNTAC 2 cut(s) 852, 884
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 909
BbvI GCAGC 9 cut(s) 235, 238, 325, 328, 686, 776, 953, 1213, 1424
BccI CCATC 6 cut(s) 508, 655, 688, 709, 943, 1451
BceAI ACGGC 1 cut(s) 1302
BciT130I CCWGG 1 cut(s) 690
BciVI GTATCC 1 cut(s) 916
BclI TGATCA 2 cut(s) 552, 1441
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 3 cut(s) 345, 377, 1404
BfmI CTRYAG 1 cut(s) 910
BfoI RGCGCY 1 cut(s) 444
BfuI GTATCC 1 cut(s) 916
BmcAI AGTACT 1 cut(s) 889
Bme1390I CCNGG 1 cut(s) 690
Bme18I GGWCC 1 cut(s) 1364
BmgT120I GGNCC 1 cut(s) 1364
BmiI GGNNCC 3 cut(s) 442, 1266, 1365
BmrFI CCNGG 1 cut(s) 690
BmsI GCATC 5 cut(s) 325, 591, 686, 810, 825
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 845
BpuEI CTTGAG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 927
BsaBI GATNNNNATC 1 cut(s) 1383
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 689, 1374
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 845, 1167
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 3 cut(s) 749, 895, 947
Bse8I GATNNNNATC 1 cut(s) 1383
BseBI CCWGG 1 cut(s) 690
BseDI CCNNGG 2 cut(s) 689, 1374
BseGI GGATG 6 cut(s) 303, 699, 720, 958, 1000, 1462
BseJI GATNNNNATC 1 cut(s) 1383
BseLI CCNNNNNNNGG 3 cut(s) 145, 845, 1167
BseMII CTCAG 3 cut(s) 183, 1257, 1388
BseNI ACTGG 3 cut(s) 749, 895, 947
BseRI GAGGAG 1 cut(s) 917
BseXI GCAGC 9 cut(s) 235, 238, 325, 328, 686, 776, 953, 1213, 1424
BseYI CCCAGC 1 cut(s) 1260
BshFI GGCC 1 cut(s) 1289
BshNI GGYRCC 2 cut(s) 440, 1264
BsiHKAI GWGCWC 1 cut(s) 909
BsiSI CCGG 1 cut(s) 179
BslI CCNNNNNNNGG 3 cut(s) 145, 845, 1167
BsmAI GTCTC 1 cut(s) 150
BsmI GAATGC 1 cut(s) 1270
BsnI GGCC 1 cut(s) 1289
Bso31I GGTCTC 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 909
Bsp143I GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
BspACI CCGC 4 cut(s) 82, 139, 469, 1268
BspANI GGCC 1 cut(s) 1289
BspCNI CTCAG 3 cut(s) 182, 1256, 1389
BspLI GGNNCC 3 cut(s) 442, 1266, 1365
BspPI GGATC 2 cut(s) 312, 719
BspT107I GGYRCC 2 cut(s) 440, 1264
BspTNI GGTCTC 1 cut(s) 150
BsrBI CCGCTC 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 3 cut(s) 749, 895, 947
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 689, 1374
BssMI GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
BssNI GRCGYC 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 690
Bst6I CTCTTC 3 cut(s) 609, 846, 1329
BstACI GRCGYC 1 cut(s) 441
BstAPI GCANNNNNTGC 2 cut(s) 671, 1207
BstBAI YACGTR 1 cut(s) 927
BstC8I GCNNGC 3 cut(s) 23, 384, 1162
BstDEI CTNAG 4 cut(s) 169, 1243, 1397, 1505
BstENI CCTNNNNNAGG 1 cut(s) 843
BstF5I GGATG 6 cut(s) 303, 699, 720, 958, 1000, 1462
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 7 cut(s) 307, 555, 591, 727, 975, 1341, 1444
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
BstMWI GCNNNNNNNGC 8 cut(s) 235, 244, 440, 671, 820, 1072, 1207, 1409
BstNI CCWGG 1 cut(s) 690
BstNSI RCATGY 3 cut(s) 386, 550, 688
BstSCI CCNGG 1 cut(s) 688
BstSFI CTRYAG 1 cut(s) 910
BstSNI TACGTA 1 cut(s) 927
BstV1I GCAGC 9 cut(s) 235, 238, 325, 328, 686, 776, 953, 1213, 1424
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 724
BstYI RGATCY 1 cut(s) 724
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 1 cut(s) 1289
BtsCI GGATG 6 cut(s) 303, 699, 720, 958, 1000, 1462
BtsIMutI CAGTG 3 cut(s) 205, 954, 1251
Cac8I GCNNGC 3 cut(s) 23, 384, 1162
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 1 cut(s) 443
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 1364
CseI GACGC 1 cut(s) 273
Csp6I GTAC 4 cut(s) 640, 888, 928, 956
CviAII CATG 9 cut(s) 16, 134, 371, 383, 496, 547, 685, 970, 1167
CviQI GTAC 4 cut(s) 640, 888, 928, 956
DdeI CTNAG 4 cut(s) 169, 1243, 1397, 1505
DinI GGCGCC 1 cut(s) 442
DpnI GATC 7 cut(s) 306, 554, 590, 726, 974, 1340, 1443
DpnII GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
DraI TTTAAA 1 cut(s) 420
Eam1104I CTCTTC 3 cut(s) 609, 846, 1329
EarI CTCTTC 3 cut(s) 609, 846, 1329
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 907
Eco105I TACGTA 1 cut(s) 927
Eco24I GRGCYC 1 cut(s) 909
Eco31I GGTCTC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 1364
Eco53kI GAGCTC 1 cut(s) 907
Eco57I CTGAAG 3 cut(s) 215, 836, 1032
EcoICRI GAGCTC 1 cut(s) 907
EcoNI CCTNNNNNAGG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 1364
EcoRI GAATTC 2 cut(s) 124, 624
EcoRII CCWGG 1 cut(s) 688
EcoRV GATATC 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 909
EgeI GGCGCC 1 cut(s) 442
EheI GGCGCC 1 cut(s) 442
FaeI CATG 9 cut(s) 19, 137, 374, 386, 499, 550, 688, 973, 1170
FalI AAGNNNNNCTT 2 cut(s) 1138, 1170
FatI CATG 9 cut(s) 15, 133, 370, 382, 495, 546, 684, 969, 1166
FauNDI CATATG 2 cut(s) 436, 961
FbaI TGATCA 2 cut(s) 552, 1441
FblI GTMKAC 1 cut(s) 741
FokI GGATG 6 cut(s) 310, 706, 727, 965, 1007, 1469
FriOI GRGCYC 1 cut(s) 909
FspBI CTAG 3 cut(s) 345, 377, 1404
GlaI GCGC 1 cut(s) 442
GsaI CCCAGC 1 cut(s) 1264
GsuI CTGGAG 1 cut(s) 845
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 1 cut(s) 1289
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 273
HhaI GCGC 1 cut(s) 443
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 9 cut(s) 19, 137, 374, 386, 499, 550, 688, 973, 1170
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HincII GTYRAC 2 cut(s) 742, 792
HindII GTYRAC 2 cut(s) 742, 792
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 4 cut(s) 874, 1034, 1118, 1149
HpaI GTTAAC 1 cut(s) 792
HpaII CCGG 1 cut(s) 179
Hpy166II GTNNAC 6 cut(s) 642, 742, 792, 1129, 1349, 1464
Hpy188I TCNGA 6 cut(s) 155, 682, 873, 1375, 1398, 1446
Hpy188III TCNNGA 3 cut(s) 250, 281, 1439
Hpy8I GTNNAC 6 cut(s) 642, 742, 792, 1129, 1349, 1464
Hpy99I CGWCG 1 cut(s) 743
HpyCH4IV ACGT 2 cut(s) 644, 926
HpyF10VI GCNNNNNNNGC 8 cut(s) 235, 244, 440, 671, 820, 1072, 1207, 1409
HpyF3I CTNAG 4 cut(s) 169, 1243, 1397, 1505
HpySE526I ACGT 2 cut(s) 644, 926
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 9 cut(s) 19, 137, 374, 386, 499, 550, 688, 973, 1170
HspAI GCGC 1 cut(s) 441
KasI GGCGCC 1 cut(s) 440
Ksp22I TGATCA 2 cut(s) 552, 1441
KspAI GTTAAC 1 cut(s) 792
Kzo9I GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
LmnI GCTCC 1 cut(s) 904
Lsp1109I GCAGC 9 cut(s) 235, 238, 325, 328, 686, 776, 953, 1213, 1424
LweI GCATC 5 cut(s) 325, 591, 686, 810, 825
MaeI CTAG 3 cut(s) 345, 377, 1404
MaeII ACGT 2 cut(s) 644, 926
MaeIII GTNAC 1 cut(s) 1035
MalI GATC 7 cut(s) 306, 554, 590, 726, 974, 1340, 1443
MbiI CCGCTC 1 cut(s) 469
MboI GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 1 cut(s) 909
Mly113I GGCGCC 1 cut(s) 441
MlyI GAGTC 2 cut(s) 868, 1043
MmeI TCCRAC 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 455
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 6 cut(s) 9, 356, 419, 429, 734, 791
MslI CAYNNNNRTG 2 cut(s) 458, 599
MspA1I CMGCKG 1 cut(s) 1415
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 690
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 1270
MvaI CCWGG 1 cut(s) 690
MwoI GCNNNNNNNGC 8 cut(s) 235, 244, 440, 671, 820, 1072, 1207, 1409
NarI GGCGCC 1 cut(s) 441
NdeI CATATG 2 cut(s) 436, 961
NdeII GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
NlaIII CATG 9 cut(s) 19, 137, 374, 386, 499, 550, 688, 973, 1170
NlaIV GGNNCC 3 cut(s) 442, 1266, 1365
NmuCI GTSAC 1 cut(s) 1035
NsiI ATGCAT 1 cut(s) 455
NspI RCATGY 3 cut(s) 386, 550, 688
PaeI GCATGC 1 cut(s) 386
PciI ACATGT 1 cut(s) 546
PctI GAATGC 1 cut(s) 1270
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 2 cut(s) 1118, 1149
PflMI CCANNNNNTGG 1 cut(s) 1167
PleI GAGTC 2 cut(s) 868, 1042
PluTI GGCGCC 1 cut(s) 444
PpsI GAGTC 2 cut(s) 868, 1042
Ppu21I YACGTR 1 cut(s) 927
PpuMI RGGWCCY 1 cut(s) 1364
PscI ACATGT 1 cut(s) 546
Psp124BI GAGCTC 1 cut(s) 909
Psp5II RGGWCCY 1 cut(s) 1364
Psp6I CCWGG 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 1260
PspGI CCWGG 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 442, 1266, 1365
PspPI GGNCC 1 cut(s) 1364
PspPPI RGGWCCY 1 cut(s) 1364
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 724
PvuII CAGCTG 1 cut(s) 1415
RsaI GTAC 4 cut(s) 641, 889, 929, 957
RsaNI GTAC 4 cut(s) 640, 888, 928, 956
RseI CAYNNNNRTG 2 cut(s) 458, 599
SacI GAGCTC 1 cut(s) 909
SalI GTCGAC 1 cut(s) 740
SaqAI TTAA 6 cut(s) 9, 356, 419, 429, 734, 791
Sau3AI GATC 7 cut(s) 304, 552, 588, 724, 972, 1338, 1441
Sau96I GGNCC 1 cut(s) 1364
ScaI AGTACT 1 cut(s) 889
SchI GAGTC 2 cut(s) 868, 1043
ScrFI CCNGG 1 cut(s) 690
SduI GDGCHC 1 cut(s) 909
SfaNI GCATC 5 cut(s) 325, 591, 686, 810, 825
SfcI CTRYAG 1 cut(s) 910
SfoI GGCGCC 1 cut(s) 442
SinI GGWCC 1 cut(s) 1364
SmiMI CAYNNNNRTG 2 cut(s) 458, 599
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SnaBI TACGTA 1 cut(s) 927
SphI GCATGC 1 cut(s) 386
SsiI CCGC 4 cut(s) 82, 139, 469, 1268
SspDI GGCGCC 1 cut(s) 440
SspMI CTAG 3 cut(s) 345, 377, 1404
SstI GAGCTC 1 cut(s) 909
StyD4I CCNGG 1 cut(s) 688
TaiI ACGT 2 cut(s) 647, 929
TaqI TCGA 5 cut(s) 52, 122, 655, 741, 938
TatI WGTACW 2 cut(s) 639, 887
TauI GCSGC 1 cut(s) 1270
TfiI GAWTC 2 cut(s) 1118, 1149
Tru1I TTAA 6 cut(s) 9, 356, 419, 429, 734, 791
Tru9I TTAA 6 cut(s) 9, 356, 419, 429, 734, 791
TscAI CASTG 3 cut(s) 205, 954, 1251
TseFI GTSAC 1 cut(s) 1035
TseI GCWGC 9 cut(s) 223, 226, 338, 341, 674, 764, 966, 1201, 1412
Tsp45I GTSAC 1 cut(s) 1035
TspDTI ATGAA 6 cut(s) 17, 359, 521, 957, 1463, 1488
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 3 cut(s) 205, 954, 1251
Van91I CCANNNNNTGG 1 cut(s) 1167
VpaK11BI GGWCC 1 cut(s) 1364
XagI CCTNNNNNAGG 1 cut(s) 843
XapI RAATTY 3 cut(s) 124, 624, 863
XceI RCATGY 3 cut(s) 386, 550, 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 741
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 3 cut(s) 345, 377, 1404
ZrmI AGTACT 1 cut(s) 889
Zsp2I ATGCAT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.