Rroxscaffold_2G00117250

Pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018494
Physical Location & Seq
Reverse (-)
44767556 .. 44768257
702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117250.1

Sequence Viewer

Length: 609 bp
ATGGGAGAAGACTTGTTTTCAGTGTCGGATTACAATCCCACAATTGCAAACAAAGTTCTTAACAAGATTTCCTTATGTGACACGTTTACTTTAGCAAACCGAATGGCGGCATATATTGCAGCATTTGACATGCCTGGGATGGAAACTATCCTGAACAGAACGGAGGAAGATTCATACTTCATTGTTTACTGGAAAATCTATTCAATGGCAGCAAGTGGTTATCTAATCGATCCAACTTTAACAAAGGCTCTTTCAATGCTAAAGAAAATGGAGAGAATGATGCCTAAGGGAGGAAATCGTAAAGAGTTTCTTATCACTCTTTATGCAAGTACTGGCCATAAAGAGGAGCTGGATGATATTAAGGAAGCTGAGAGGATATTTGCGGAGTGGGAGTCACAGTGCAAAATGTATGACTTTCGGGTGCTGAACAGGCTTCTTATTGTCTATTGCAAAATGGGTCTTTTGGACAAGGCAGAATCACTTGTAAACGAGGCTGTGGAAGGAAGAATTCCTTATGCCAGCACATGGCATGTGCTAGCAATAGATTTTATGGAGAATAAGCAGATACCCAGGGCAGTTGAGATGTTGAAGAATACTTTCAGTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

202

Amino Acids

23.34

Weight (kDa)

5.36

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018494)

Species Orthologous Gene IDs
malus_domestica MD09G1248900.v1.1
prunus_persica Prupe.3G136700_v2.0.a1
pyrus_communis pycom09g16580
rosa_chinensis RchiOBHm_Chr2g0126311
rosa_laevigata RLG00000018900
rosa_multiflora Rmu_ssc0000264.1_g000039
rosa_roxburghii Rroxscaffold_2G00117250
rosa_samantha Rh2AG319400 Rh2BG327800 Rh2DG347500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 525
AciI CCGC 2 cut(s) 107, 383
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 1 cut(s) 507
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 4 cut(s) 106, 290, 343, 525
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 3 cut(s) 204, 255, 589
AjnI CCWGG 2 cut(s) 133, 569
AluBI AGCT 2 cut(s) 349, 368
AluI AGCT 2 cut(s) 349, 368
AlwI GGATC 1 cut(s) 224
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 2 cut(s) 119, 209
ApoI RAATTY 1 cut(s) 507
Asp700I GAANNNNTTC 1 cut(s) 596
AsuNHI GCTAGC 1 cut(s) 535
AxyI CCTNAGG 1 cut(s) 285
BalI TGGCCA 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 131, 221
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 2 cut(s) 135, 571
BfaI CTAG 1 cut(s) 536
BisI GCNGC 3 cut(s) 108, 120, 210
BlsI GCNGC 3 cut(s) 109, 121, 211
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 2 cut(s) 135, 571
BmrFI CCNGG 2 cut(s) 135, 571
BmsI GCATC 1 cut(s) 270
BmtI GCTAGC 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 134, 569, 570
BsaXI ACNNNNNCTCC 4 cut(s) 377, 383, 407, 413
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 290, 343, 525
Bse1I ACTGG 2 cut(s) 194, 337
Bse21I CCTNAGG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 2 cut(s) 135, 571
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 134, 569, 570
BseGI GGATG 2 cut(s) 144, 358
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 4 cut(s) 106, 290, 343, 525
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 194, 337
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 2 cut(s) 131, 221
BshFI GGCC 1 cut(s) 336
BshVI ATCGAT 1 cut(s) 228
BslI CCNNNNNNNGG 4 cut(s) 106, 290, 343, 525
BsnI GGCC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 229
BspACI CCGC 2 cut(s) 107, 383
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 361
BspDI ATCGAT 1 cut(s) 228
BspOI GCTAGC 1 cut(s) 539
BspPI GGATC 1 cut(s) 224
BsrI ACTGG 2 cut(s) 194, 337
BssECI CCNNGG 3 cut(s) 134, 569, 570
BssMI GATC 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 135, 571
Bst4CI ACNGT 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 520, 537
BstDEI CTNAG 2 cut(s) 285, 369
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 144, 358
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 116, 430
BstNI CCWGG 2 cut(s) 135, 571
BstNSI RCATGY 2 cut(s) 133, 533
BstSCI CCNGG 2 cut(s) 133, 569
BstV1I GCAGC 2 cut(s) 131, 221
BstV2I GAAGAC 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 1 cut(s) 336
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 2 cut(s) 144, 358
BtsIMutI CAGTG 2 cut(s) 27, 404
Cac8I GCNNGC 2 cut(s) 520, 537
ClaI ATCGAT 1 cut(s) 228
Csp6I GTAC 1 cut(s) 330
CviAII CATG 3 cut(s) 130, 525, 530
CviJI RGCY 6 cut(s) 248, 336, 349, 368, 433, 494
CviKI_1 RGCY 6 cut(s) 248, 336, 349, 368, 433, 494
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 285, 369
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 334
Eco81I CCTNAGG 1 cut(s) 285
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 507
EcoRII CCWGG 2 cut(s) 133, 569
FaeI CATG 3 cut(s) 133, 528, 533
FalI AAGNNNNNCTT 6 cut(s) 56, 88, 294, 326, 496, 528
FatI CATG 3 cut(s) 129, 524, 529
Fnu4HI GCNGC 3 cut(s) 108, 120, 210
FokI GGATG 2 cut(s) 151, 365
Fsp4HI GCNGC 3 cut(s) 108, 120, 210
FspBI CTAG 1 cut(s) 536
GluI GCNGC 3 cut(s) 108, 120, 210
HaeIII GGCC 1 cut(s) 336
Hin1II CATG 3 cut(s) 133, 528, 533
HinfI GANTC 3 cut(s) 170, 392, 476
Hpy166II GTNNAC 3 cut(s) 87, 187, 487
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 1 cut(s) 151
Hpy8I GTNNAC 3 cut(s) 87, 187, 487
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 5 cut(s) 47, 119, 326, 402, 450
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 430
HpyF3I CTNAG 2 cut(s) 285, 369
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 3 cut(s) 133, 528, 533
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 2 cut(s) 131, 221
LweI GCATC 1 cut(s) 270
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 77, 393
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 4 cut(s) 20, 179, 516, 601
MfeI CAATTG 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 2 cut(s) 42, 507
MluNI TGGCCA 1 cut(s) 336
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 2 cut(s) 6, 257
MnlI CCTC 5 cut(s) 157, 284, 337, 366, 484
Mox20I TGGCCA 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 596
MscI TGGCCA 1 cut(s) 336
MseI TTAA 3 cut(s) 60, 239, 360
Msp20I TGGCCA 1 cut(s) 336
MspR9I CCNGG 2 cut(s) 135, 571
MunI CAATTG 1 cut(s) 42
MvaI CCWGG 2 cut(s) 135, 571
MwoI GCNNNNNNNGC 2 cut(s) 116, 430
NdeII GATC 1 cut(s) 229
NheI GCTAGC 1 cut(s) 535
NlaIII CATG 3 cut(s) 133, 528, 533
NmuCI GTSAC 2 cut(s) 77, 393
NspI RCATGY 2 cut(s) 133, 533
PasI CCCWGGG 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 2 cut(s) 170, 476
PflMI CCANNNNNTGG 1 cut(s) 525
PkrI GCNGC 3 cut(s) 109, 121, 211
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Psp6I CCWGG 2 cut(s) 133, 569
PspGI CCWGG 2 cut(s) 133, 569
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SaqAI TTAA 3 cut(s) 60, 239, 360
SatI GCNGC 3 cut(s) 108, 120, 210
Sau3AI GATC 1 cut(s) 229
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 135, 571
SetI ASST 3 cut(s) 86, 351, 370
SfaNI GCATC 1 cut(s) 270
Sse9I AATT 2 cut(s) 42, 507
SsiI CCGC 2 cut(s) 107, 383
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 133, 569
TaaI ACNGT 1 cut(s) 399
TaiI ACGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 228
TasI AATT 2 cut(s) 42, 507
TatI WGTACW 1 cut(s) 329
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 170, 476
Tru1I TTAA 3 cut(s) 60, 239, 360
Tru9I TTAA 3 cut(s) 60, 239, 360
TscAI CASTG 2 cut(s) 27, 404
TseFI GTSAC 2 cut(s) 77, 393
TseI GCWGC 2 cut(s) 119, 209
Tsp45I GTSAC 2 cut(s) 77, 393
TspDTI ATGAA 2 cut(s) 162, 169
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 27, 404
Van91I CCANNNNNTGG 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 288
XapI RAATTY 1 cut(s) 507
XceI RCATGY 2 cut(s) 133, 533
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 1 cut(s) 536
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.