Prupe.3G136700_v2.0.a1

Pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0018494
Physical Location & Seq
Forward (+)
14402856 .. 14405871
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G136700.1

Sequence Viewer

Length: 1545 bp
ATGAAGCTTAGTTGTATGAGCAAGCTCATATCCTTTTCCCTTCAAAACCCTAGCAGAAATTATGGTGCTTTACCTTCACTCCGCCCTTCTAAAATCCCAAACCCACCTTCTCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATCAACTACTCTCCACGATCGCATCAAGGTCATCAGAGACCCAAAAGCCTCGGTTCTACCAGTGCTCGAACAGTGGGTCACTGAAGGTGGAGCTGTTGAGAAGCAAGAGCTTCAGTCACTGGTTCGACTCTTGAAGGACTTCAGACGCTTCAATCACGCTCTTGAGATATCCCAGTGGATGACGGATCGCAGGTATTTTGATTTATCACCAAGTGATGCTGCGGCTAGATTGAACTTAATCCACAGAGTGCATGGACTAGAGCATGCAGAAAAGTACTTCAACAATTTGTCGAAGAATTTAAAATCAGTTAATGCATATGGTGCTCTTCTCAGCATTTATGTGCAAGAGCATTCTGTTGAGAAAGCAGAAGCCACTATGCAGAAAATGAAAAAGATGGGTATGGCAAAGACATCATTCCCTTACAATATGTTGATCAATCTTTACTCTCAAAATGGAGAACATGAGAAGATTAACATACTGATGCAAGAAATGGAAGAGAATGGAATTCCTCTCGACAAGTACACTTTGAGAAATCGAATGATTGCATACATTGCAGCATCTGACATGCCTGGGATGGAAACTATCCTGAATAGGATGGAGGAAGATTCAAACTTCATTGTTGACTGGAAAATATATTCAATGGCAGCAAGTGGGTATCTAAAAGTCGGGATGATAAAAAAGGCTTGTTCAATGCTGAAAATGATGGAGAGAATGATGCCTCTCCAAGGGAGAAAATCATTAGAATTTCTTATCACTCTTTATGCAAATACTGGCCATAAAGGGGAGCTATACAGAGTTTGGAATACATACAAGCAATCAAATGAACCAATGGATGTACCATATGGTTGCATGATCTCATCTTTGGCAAAGCTTGATGATATTGAGGGGGCTGAGAGGATATTTGAGGAGTGGGAGTCACGATGCACAGAGTATTATGACTTTCGGGTGTTGAACAGGCTTCTTGTTGCGTATTGCAAAAAGGGTCTTTTTGATAAAGCAGAATCAGCTGTAAAGAAGGCTGTGGAAGGAAGAGTCCCATATGCTAGCACATGGAATGCGTTAGCAATAGGTTATACGGAAAGTAAGCAAATGCCTAAAGCAATTGAAACATTGAAGAAGGCACTGTCAGTTGATAGGCGGGGCTGGGTGCCTGATTCCAGTACTCTGACAGCATGTTTGGATTATTTGGAAGGGCAAGGAGATTTTGAAGGAATTGAAGAGATCATAAGTTTACTAAAGAACTTGGGTCCTTTGTCCAGGGATTTGTATCAGAGACTGCTGAGGGCTAGTGCTGCATCTGGAAAATCAGTTTCTATAATTCTTGATCAGATGAAAGTGGATGGTTTTACTGCTGATGAAGAAGCATACAAGTTTCTTGAAACAGGGCCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

515

Amino Acids

58.18

Weight (kDa)

8.46

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018494)

Species Orthologous Gene IDs
malus_domestica MD09G1248900.v1.1
prunus_persica Prupe.3G136700_v2.0.a1
pyrus_communis pycom09g16580
rosa_chinensis RchiOBHm_Chr2g0126311
rosa_laevigata RLG00000018900
rosa_multiflora Rmu_ssc0000264.1_g000039
rosa_roxburghii Rroxscaffold_2G00117250
rosa_samantha Rh2AG319400 Rh2BG327800 Rh2DG347500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 330
AccB1I GGYRCC 1 cut(s) 1297
AciI CCGC 3 cut(s) 82, 371, 1288
AclWI GGATC 1 cut(s) 342
AcoI YGGCCR 1 cut(s) 922
AcsI RAATTY 3 cut(s) 445, 654, 893
AcuI CTGAAG 3 cut(s) 245, 252, 274
AdeI CACNNNGTG 2 cut(s) 362, 397
AfaI GTAC 4 cut(s) 425, 671, 987, 1312
AfiI CCNNNNNNNGG 2 cut(s) 875, 931
AjnI CCWGG 2 cut(s) 718, 1406
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
AluBI AGCT 7 cut(s) 7, 25, 242, 259, 937, 1021, 1157
AluI AGCT 7 cut(s) 7, 25, 242, 259, 937, 1021, 1157
Alw21I GWGCWC 2 cut(s) 216, 475
Alw26I GTCTC 2 cut(s) 180, 1417
AlwI GGATC 1 cut(s) 342
AlwNI CAGNNNCTG 3 cut(s) 268, 710, 1426
AoxI GGCC 2 cut(s) 922, 1535
ApeKI GCWGC 4 cut(s) 368, 704, 794, 1442
ApoI RAATTY 3 cut(s) 445, 654, 893
ArsI GACNNNNNNTTYG 4 cut(s) 210, 242, 1309, 1341
Asp700I GAANNNNTTC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 1397, 1535
AsuHPI GGTGA 1 cut(s) 348
AsuNHI GCTAGC 1 cut(s) 1193
AvaII GGWCC 1 cut(s) 1397
BalI TGGCCA 1 cut(s) 924
BanI GGYRCC 1 cut(s) 1297
Bbv12I GWGCWC 2 cut(s) 216, 475
BbvCI CCTCAGC 1 cut(s) 1430
BbvI GCAGC 4 cut(s) 355, 716, 806, 1429
BccI CCATC 5 cut(s) 538, 718, 739, 847, 1484
BciT130I CCWGG 2 cut(s) 720, 1408
BclI TGATCA 2 cut(s) 582, 1474
BcoDI GTCTC 2 cut(s) 180, 1417
BfaI CTAG 5 cut(s) 51, 375, 407, 1194, 1437
BfuAI ACCTGC 1 cut(s) 330
BisI GCNGC 5 cut(s) 369, 372, 705, 795, 1443
BlsI GCNGC 5 cut(s) 370, 373, 706, 796, 1444
BmcAI AGTACT 2 cut(s) 425, 1312
Bme1390I CCNGG 2 cut(s) 720, 1408
Bme18I GGWCC 1 cut(s) 1397
BmgT120I GGNCC 2 cut(s) 1397, 1535
BmiI GGNNCC 2 cut(s) 1299, 1398
BmrFI CCNGG 2 cut(s) 720, 1408
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 7 cut(s) 180, 355, 621, 716, 855, 1061, 1454
BmtI GCTAGC 1 cut(s) 1197
BmuI ACTGGG 1 cut(s) 316
Bpu10I CCTNAGC 1 cut(s) 1430
BpuEI CTTGAG 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 1416
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 198, 719, 874, 1407
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1049, 1079
Bsc4I CCNNNNNNNGG 2 cut(s) 875, 931
Bse1I ACTGG 6 cut(s) 209, 273, 322, 779, 925, 1308
Bse3DI GCAATG 1 cut(s) 699
Bse8I GATNNNNATC 1 cut(s) 1416
BseBI CCWGG 2 cut(s) 720, 1408
BseDI CCNNGG 4 cut(s) 198, 719, 874, 1407
BseGI GGATG 6 cut(s) 333, 729, 750, 825, 988, 1495
BseJI GATNNNNATC 1 cut(s) 1416
BseLI CCNNNNNNNGG 2 cut(s) 875, 931
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 3 cut(s) 493, 1032, 1421
BseNI ACTGG 6 cut(s) 209, 273, 322, 779, 925, 1308
BseRI GAGGAG 1 cut(s) 1070
BseXI GCAGC 4 cut(s) 355, 716, 806, 1429
BseYI CCCAGC 1 cut(s) 1293
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 924, 1537
BshNI GGYRCC 1 cut(s) 1297
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 216, 475
BslFI GGGAC 1 cut(s) 1169
BslI CCNNNNNNNGG 2 cut(s) 875, 931
BsmAI GTCTC 2 cut(s) 180, 1417
BsmFI GGGAC 1 cut(s) 1169
BsmI GAATGC 2 cut(s) 499, 1210
BsnI GGCC 2 cut(s) 924, 1537
Bso31I GGTCTC 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 216, 475
Bsp143I GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
BspACI CCGC 3 cut(s) 82, 371, 1288
BspANI GGCC 2 cut(s) 924, 1537
BspCNI CTCAG 3 cut(s) 492, 1033, 1422
BspLI GGNNCC 2 cut(s) 1299, 1398
BspMI ACCTGC 1 cut(s) 330
BspOI GCTAGC 1 cut(s) 1197
BspPI GGATC 1 cut(s) 342
BspQI GCTCTTC 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 1297
BspTNI GGTCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 699
BsrI ACTGG 6 cut(s) 209, 273, 322, 779, 925, 1308
BssECI CCNNGG 4 cut(s) 198, 719, 874, 1407
BssMI GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
BssT1I CCWWGG 1 cut(s) 874
Bst2UI CCWGG 2 cut(s) 720, 1408
Bst4CI ACNGT 2 cut(s) 222, 1275
Bst6I CTCTTC 4 cut(s) 480, 639, 1174, 1362
BstAPI GCANNNNNTGC 2 cut(s) 470, 701
BstC8I GCNNGC 3 cut(s) 23, 414, 1195
BstDEI CTNAG 4 cut(s) 8, 479, 1041, 1430
BstENI CCTNNNNNAGG 1 cut(s) 873
BstF5I GGATG 6 cut(s) 333, 729, 750, 825, 988, 1495
BstKTI GATC 6 cut(s) 169, 337, 585, 1005, 1374, 1477
BstMAI GTCTC 2 cut(s) 180, 1417
BstMBI GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 4 cut(s) 470, 701, 1154, 1442
BstNI CCWGG 2 cut(s) 720, 1408
BstNSI RCATGY 3 cut(s) 416, 718, 1326
BstSCI CCNGG 2 cut(s) 718, 1406
BstV1I GCAGC 4 cut(s) 355, 716, 806, 1429
BsuRI GGCC 2 cut(s) 924, 1537
BtsCI GGATG 6 cut(s) 333, 729, 750, 825, 988, 1495
BtsIMutI CAGTG 6 cut(s) 216, 227, 228, 266, 329, 1271
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 3 cut(s) 23, 414, 1195
CaiI CAGNNNCTG 3 cut(s) 268, 710, 1426
Cfr13I GGNCC 2 cut(s) 1397, 1535
CseI GACGC 1 cut(s) 303
Csp6I GTAC 4 cut(s) 424, 670, 986, 1311
CviAII CATG 7 cut(s) 401, 413, 611, 715, 1000, 1200, 1323
CviQI GTAC 4 cut(s) 424, 670, 986, 1311
DdeI CTNAG 4 cut(s) 8, 479, 1041, 1430
DpnI GATC 6 cut(s) 168, 336, 584, 1004, 1373, 1476
DpnII GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
DraI TTTAAA 1 cut(s) 450
DraIII CACNNNGTG 2 cut(s) 362, 397
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 4 cut(s) 480, 639, 1174, 1362
EarI CTCTTC 4 cut(s) 480, 639, 1174, 1362
EciI GGCGGA 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 874
Eco31I GGTCTC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 1397
Eco57I CTGAAG 3 cut(s) 245, 252, 274
EcoNI CCTNNNNNAGG 1 cut(s) 873
EcoO109I RGGNCCY 1 cut(s) 1397
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 2 cut(s) 718, 1406
EcoRV GATATC 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 874
EcoT22I ATGCAT 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 874
FaeI CATG 7 cut(s) 404, 416, 614, 718, 1003, 1203, 1326
FaqI GGGAC 1 cut(s) 1169
FatI CATG 7 cut(s) 400, 412, 610, 714, 999, 1199, 1322
FauI CCCGC 1 cut(s) 1281
FauNDI CATATG 3 cut(s) 466, 991, 1189
FbaI TGATCA 2 cut(s) 582, 1474
Fnu4HI GCNGC 5 cut(s) 369, 372, 705, 795, 1443
FokI GGATG 6 cut(s) 340, 736, 757, 832, 995, 1502
Fsp4HI GCNGC 5 cut(s) 369, 372, 705, 795, 1443
FspBI CTAG 5 cut(s) 51, 375, 407, 1194, 1437
GluI GCNGC 5 cut(s) 369, 372, 705, 795, 1443
GsaI CCCAGC 1 cut(s) 1297
HaeIII GGCC 2 cut(s) 924, 1537
HgaI GACGC 1 cut(s) 303
Hin1II CATG 7 cut(s) 404, 416, 614, 718, 1003, 1203, 1326
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 2 cut(s) 5, 1019
HinfI GANTC 6 cut(s) 276, 755, 1064, 1151, 1182, 1304
HphI GGTGA 1 cut(s) 348
Hpy166II GTNNAC 3 cut(s) 672, 772, 1382
Hpy188I TCNGA 6 cut(s) 185, 293, 712, 1317, 1422, 1479
Hpy188III TCNNGA 9 cut(s) 280, 311, 662, 736, 817, 1068, 1449, 1472, 1526
Hpy8I GTNNAC 3 cut(s) 672, 772, 1382
HpyCH4III ACNGT 2 cut(s) 222, 1275
HpyF10VI GCNNNNNNNGC 4 cut(s) 470, 701, 1154, 1442
HpyF3I CTNAG 4 cut(s) 8, 479, 1041, 1430
Hsp92II CATG 7 cut(s) 404, 416, 614, 718, 1003, 1203, 1326
Ksp22I TGATCA 2 cut(s) 582, 1474
Kzo9I GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 2 cut(s) 239, 934
Lsp1109I GCAGC 4 cut(s) 355, 716, 806, 1429
LweI GCATC 7 cut(s) 180, 355, 621, 716, 855, 1061, 1454
MaeI CTAG 5 cut(s) 51, 375, 407, 1194, 1437
MaeIII GTNAC 3 cut(s) 226, 264, 1065
MalI GATC 6 cut(s) 168, 336, 584, 1004, 1373, 1476
MboI GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
MfeI CAATTG 1 cut(s) 1251
MhlI GDGCHC 2 cut(s) 216, 475
MlsI TGGCCA 1 cut(s) 924
MluCI AATT 8 cut(s) 58, 433, 445, 654, 893, 1251, 1362, 1467
MluNI TGGCCA 1 cut(s) 924
MlyI GAGTC 3 cut(s) 270, 1073, 1191
MnlI CCTC 8 cut(s) 208, 669, 742, 879, 1027, 1038, 1048, 1425
Mox20I TGGCCA 1 cut(s) 924
Mph1103I ATGCAT 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 287
MscI TGGCCA 1 cut(s) 924
MseI TTAA 4 cut(s) 386, 449, 459, 621
MslI CAYNNNNRTG 2 cut(s) 488, 629
Msp20I TGGCCA 1 cut(s) 924
MspA1I CMGCKG 1 cut(s) 1157
MspR9I CCNGG 2 cut(s) 720, 1408
MunI CAATTG 1 cut(s) 1251
Mva1269I GAATGC 2 cut(s) 499, 1210
MvaI CCWGG 2 cut(s) 720, 1408
MwoI GCNNNNNNNGC 4 cut(s) 470, 701, 1154, 1442
NdeI CATATG 3 cut(s) 466, 991, 1189
NdeII GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
NheI GCTAGC 1 cut(s) 1193
NlaIII CATG 7 cut(s) 404, 416, 614, 718, 1003, 1203, 1326
NlaIV GGNNCC 2 cut(s) 1299, 1398
NmuCI GTSAC 3 cut(s) 226, 264, 1065
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 3 cut(s) 416, 718, 1326
PaeI GCATGC 1 cut(s) 416
PciSI GCTCTTC 1 cut(s) 480
PctI GAATGC 2 cut(s) 499, 1210
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 3 cut(s) 755, 1151, 1304
PkrI GCNGC 5 cut(s) 370, 373, 706, 796, 1444
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 3 cut(s) 270, 1072, 1190
PpsI GAGTC 3 cut(s) 270, 1072, 1190
PpuMI RGGWCCY 1 cut(s) 1397
Psp5II RGGWCCY 1 cut(s) 1397
Psp6I CCWGG 2 cut(s) 718, 1406
PspFI CCCAGC 1 cut(s) 1293
PspGI CCWGG 2 cut(s) 718, 1406
PspN4I GGNNCC 2 cut(s) 1299, 1398
PspPI GGNCC 2 cut(s) 1397, 1535
PspPPI RGGWCCY 1 cut(s) 1397
PstNI CAGNNNCTG 3 cut(s) 268, 710, 1426
PvuI CGATCG 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 4 cut(s) 425, 671, 987, 1312
RsaNI GTAC 4 cut(s) 424, 670, 986, 1311
RseI CAYNNNNRTG 2 cut(s) 488, 629
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 4 cut(s) 386, 449, 459, 621
SatI GCNGC 5 cut(s) 369, 372, 705, 795, 1443
Sau3AI GATC 6 cut(s) 166, 334, 582, 1002, 1371, 1474
Sau96I GGNCC 2 cut(s) 1397, 1535
ScaI AGTACT 2 cut(s) 425, 1312
SchI GAGTC 3 cut(s) 270, 1073, 1191
ScrFI CCNGG 2 cut(s) 720, 1408
SduI GDGCHC 2 cut(s) 216, 475
SfaNI GCATC 7 cut(s) 180, 355, 621, 716, 855, 1061, 1454
SinI GGWCC 1 cut(s) 1397
SmiMI CAYNNNNRTG 2 cut(s) 488, 629
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SphI GCATGC 1 cut(s) 416
Sse9I AATT 8 cut(s) 58, 433, 445, 654, 893, 1251, 1362, 1467
SsiI CCGC 3 cut(s) 82, 371, 1288
SspMI CTAG 5 cut(s) 51, 375, 407, 1194, 1437
StyD4I CCNGG 2 cut(s) 718, 1406
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 2 cut(s) 222, 1275
TaqI TCGA 5 cut(s) 216, 274, 440, 663, 685
TasI AATT 8 cut(s) 58, 433, 445, 654, 893, 1251, 1362, 1467
TatI WGTACW 3 cut(s) 423, 669, 1310
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 755, 1151, 1304
Tru1I TTAA 4 cut(s) 386, 449, 459, 621
Tru9I TTAA 4 cut(s) 386, 449, 459, 621
TscAI CASTG 6 cut(s) 216, 227, 235, 273, 329, 1278
TseFI GTSAC 3 cut(s) 226, 264, 1065
TseI GCWGC 4 cut(s) 368, 704, 794, 1442
Tsp45I GTSAC 3 cut(s) 226, 264, 1065
TspDTI ATGAA 7 cut(s) 17, 138, 551, 754, 987, 1496, 1521
TspGWI ACGGA 2 cut(s) 347, 1241
TspRI CASTG 6 cut(s) 216, 227, 235, 273, 329, 1278
VpaK11BI GGWCC 1 cut(s) 1397
XagI CCTNNNNNAGG 1 cut(s) 873
XapI RAATTY 3 cut(s) 445, 654, 893
XceI RCATGY 3 cut(s) 416, 718, 1326
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 287
XspI CTAG 5 cut(s) 51, 375, 407, 1194, 1437
ZrmI AGTACT 2 cut(s) 425, 1312
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.