MD09G1260500.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011723
Physical Location & Seq
Reverse (-)
33332256 .. 33333749
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1260500.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGGTGCTCTTGATTATCTTTCAAACTTTTGCACAGTCACCAGCACAAGAAGCAAGCGTAAAGCAATGCAGACAGTTGAGATCAAAGTCAAAATGGACTGTGATGGCTGTGAAAGAAGAGTCAAGCATGCTGTTACCTCTATGAAAGGTGTAAAAACTGTGGAGGTGAACCGGAAACAAAGCCGGGTGACAGTGAGCGGGTATGTAGAACCGAACAAGGTGTTGAAGCGAGTAAAAAGCACAGGCAAGAAGAGAGCTGAGTTTTGGCCCTACATTGATCAACATCTAGTACGCTATCCCTACGCCTCTGGAGTTTACGACTGGCGGGCGCCAAAAGGCTATGTCCGGAATGTGGTTCAAGCTTTTCCGGCGTCTATAAATGCACCTGAGGAGAACATGGTCACCCTCTTCAGTGATGATAATGTCAATGCTTGTTCCATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

149

Amino Acids

16.8

Weight (kDa)

9.67

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 29 - 84 5.6e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccBSI CCGCTC 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 345
AciI CCGC 2 cut(s) 198, 325
AcuI CTGAAG 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 329, 371
AfaI GTAC 1 cut(s) 291
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 3 cut(s) 24, 226, 359
AluBI AGCT 2 cut(s) 257, 362
AluI AGCT 2 cut(s) 257, 362
Alw21I GWGCWC 1 cut(s) 10
Aor13HI TCCGGA 1 cut(s) 345
AoxI GGCC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 4 cut(s) 31, 178, 199, 394
AxyI CCTNAGG 1 cut(s) 387
BanI GGYRCC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 10
BccI CCATC 1 cut(s) 98
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 185
BfaI CTAG 1 cut(s) 287
BfoI RGCGCY 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 282
BsaHI GRCGYC 2 cut(s) 329, 371
BsaWI WCCGGW 2 cut(s) 171, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 326
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 72
Bse8I GATNNNNATC 1 cut(s) 282
BseAI TCCGGA 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 2 cut(s) 249, 378
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 404
BshFI GGCC 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 10
BsiSI CCGG 4 cut(s) 172, 184, 346, 368
BslI CCNNNNNNNGG 1 cut(s) 352
BsnI GGCC 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 10
Bsp13I TCCGGA 1 cut(s) 345
Bsp143I GATC 2 cut(s) 81, 277
BspACI CCGC 2 cut(s) 198, 325
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 2 cut(s) 250, 379
BspEI TCCGGA 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 1 cut(s) 326
BssMI GATC 2 cut(s) 81, 277
BssNI GRCGYC 2 cut(s) 329, 371
Bst4CI ACNGT 5 cut(s) 37, 76, 101, 160, 193
Bst6I CTCTTC 3 cut(s) 112, 245, 413
BstACI GRCGYC 2 cut(s) 329, 371
BstC8I GCNNGC 3 cut(s) 56, 129, 327
BstDEI CTNAG 2 cut(s) 258, 387
BstEII GGTNACC 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 84, 280
BstMBI GATC 2 cut(s) 81, 277
BstMWI GCNNNNNNNGC 2 cut(s) 51, 368
BstNSI RCATGY 1 cut(s) 131
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 387
BsuRI GGCC 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 198, 418
Cac8I GCNNGC 3 cut(s) 56, 129, 327
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 267
CseI GACGC 1 cut(s) 360
Csp6I GTAC 1 cut(s) 290
CviAII CATG 2 cut(s) 128, 397
CviJI RGCY 6 cut(s) 108, 183, 257, 268, 339, 362
CviKI_1 RGCY 6 cut(s) 108, 183, 257, 268, 339, 362
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 2 cut(s) 258, 387
DinI GGCGCC 1 cut(s) 330
DpnI GATC 2 cut(s) 83, 279
DpnII GATC 2 cut(s) 81, 277
Eam1104I CTCTTC 3 cut(s) 112, 245, 413
EarI CTCTTC 3 cut(s) 112, 245, 413
Eco57I CTGAAG 1 cut(s) 394
Eco81I CCTNAGG 1 cut(s) 387
Eco91I GGTNACC 1 cut(s) 400
EcoO65I GGTNACC 1 cut(s) 400
EgeI GGCGCC 1 cut(s) 330
EheI GGCGCC 1 cut(s) 330
FaeI CATG 2 cut(s) 131, 400
FaiI YATR 7 cut(s) 129, 143, 204, 342, 377, 398, 440
FatI CATG 2 cut(s) 127, 396
FauI CCCGC 2 cut(s) 191, 318
FbaI TGATCA 1 cut(s) 277
FspBI CTAG 1 cut(s) 287
GlaI GCGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 1 cut(s) 268
HapII CCGG 4 cut(s) 172, 184, 346, 368
HgaI GACGC 1 cut(s) 360
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 2 cut(s) 329, 371
Hin1II CATG 2 cut(s) 131, 400
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 1 cut(s) 120
HpaII CCGG 4 cut(s) 172, 184, 346, 368
HphI GGTGA 4 cut(s) 31, 178, 199, 394
Hpy166II GTNNAC 2 cut(s) 169, 316
Hpy188III TCNNGA 3 cut(s) 11, 309, 346
Hpy8I GTNNAC 2 cut(s) 169, 316
HpyCH4III ACNGT 5 cut(s) 37, 76, 101, 160, 193
HpyCH4V TGCA 3 cut(s) 33, 70, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 368
HpyF3I CTNAG 2 cut(s) 258, 387
Hsp92I GRCGYC 2 cut(s) 329, 371
Hsp92II CATG 2 cut(s) 131, 400
HspAI GCGC 1 cut(s) 329
KasI GGCGCC 1 cut(s) 328
Kpn2I TCCGGA 1 cut(s) 345
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 2 cut(s) 81, 277
LpnPI CCDG 9 cut(s) 55, 185, 197, 228, 294, 307, 359, 381, 399
MaeI CTAG 1 cut(s) 287
MaeIII GTNAC 4 cut(s) 37, 133, 187, 400
MalI GATC 2 cut(s) 83, 279
MbiI CCGCTC 1 cut(s) 198
MboI GATC 2 cut(s) 81, 277
MboII GAAGA 3 cut(s) 129, 262, 400
MhlI GDGCHC 1 cut(s) 10
Mly113I GGCGCC 1 cut(s) 329
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 5 cut(s) 148, 157, 316, 382, 416
MroI TCCGGA 1 cut(s) 345
MspI CCGG 4 cut(s) 172, 184, 346, 368
MspR9I CCNGG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 51, 368
NarI GGCGCC 1 cut(s) 329
NciI CCSGG 1 cut(s) 185
NdeII GATC 2 cut(s) 81, 277
NlaIII CATG 2 cut(s) 131, 400
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 3 cut(s) 37, 187, 400
NspI RCATGY 1 cut(s) 131
PaeI GCATGC 1 cut(s) 131
PleI GAGTC 1 cut(s) 128
PluTI GGCGCC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 128
PspEI GGTNACC 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 267
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
Sau3AI GATC 2 cut(s) 81, 277
Sau96I GGNCC 1 cut(s) 267
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 10
SetI ASST 7 cut(s) 140, 151, 168, 222, 259, 364, 388
SfoI GGCGCC 1 cut(s) 330
SphI GCATGC 1 cut(s) 131
SsiI CCGC 2 cut(s) 198, 325
SspDI GGCGCC 1 cut(s) 328
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 5 cut(s) 37, 76, 101, 160, 193
TscAI CASTG 2 cut(s) 198, 418
TseFI GTSAC 3 cut(s) 37, 187, 400
Tsp45I GTSAC 3 cut(s) 37, 187, 400
TspDTI ATGAA 1 cut(s) 158
TspRI CASTG 2 cut(s) 198, 418
XceI RCATGY 1 cut(s) 131
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.