MD09G1287800.v1.1

Protein of unknown function (DUF952)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015370
Physical Location & Seq
Reverse (-)
36776838 .. 36779537
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1287800.v1.1.491

Sequence Viewer

Length: 462 bp
ATGCAGATGGTGGAAGACTTTCAATCTCCAGACGCCAGAGCAATGGCTGCAGGTGGAGGTGGAGGTGGAGGTGGAGGTGAAGGTGAGGGGAAGGAAGAATATGTGTACAGAATCAGCACTGCTGTGGAGTGGGGAGAGTTGCAGAGCTCTGGCTCTATTTTTGGTGGAGAGCTTGACAAGAAGTCTGGGTTCATTCATCTGAGCAACCTCAACCAGGTAAAGTCGACATTGCAGAATTTTTTCGTGAATACTAAGGTGGATCTTTACTTGCTTCAAATTGACACTAATAAGCTAGGTGAAGGGTTAGTATATGAAGTTGTTGACGGTTCCAATAGCTTCCCTCATTTCTATGGTCCATCTCGAAGTTTCATTCCTCTTCCTCTTGATGCCGTTACAAAAGCAGAGAAGCTGCATCTGTCAGGTGGCCAATTCAGTTGCAGTATACTGGAACGAGCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF952

Protein Analysis

154

Amino Acids

16.4

Weight (kDa)

4.88

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF952 PF06108 38 - 133 4.2e-24 Protein of unknown function (DUF952)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015370)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01897
fragaria_vesca FvH4_1g26600
malus_domestica MD09G1287800.v1.1 MD17G1280000.v1.1
prunus_persica Prupe.3G005400_v2.0.a1
pyrus_communis pycom17g27800
rosa_chinensis RchiOBHm_Chr3g0486741
rosa_laevigata RLG00000023065
rosa_multiflora Rmu_sc0000261.1_g000016
rosa_roxburghii Rroxscaffold_6G00395640
rosa_rugosa Rorug03G0227000 Rorug03G0227000
rosa_samantha Rh3BG311500 Rh3CG310800 Rh3DG307300
rosa_wichuraiana Rw3G024610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 41
AccI GTMKAC 2 cut(s) 224, 442
AciI CCGC 1 cut(s) 456
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 1 cut(s) 235
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 2 cut(s) 23, 275
AhdI GACNNNNNGTC 1 cut(s) 181
AjnI CCWGG 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 5 cut(s) 147, 172, 292, 336, 409
AluI AGCT 5 cut(s) 147, 172, 292, 336, 409
Alw21I GWGCWC 1 cut(s) 149
AlwI GGATC 1 cut(s) 267
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 47, 409
ApoI RAATTY 1 cut(s) 235
Asp700I GAANNNNTTC 2 cut(s) 18, 239
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 89, 95, 308
AvaII GGWCC 1 cut(s) 353
BalI TGGCCA 1 cut(s) 426
BanII GRGCYC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 34, 396
BccI CCATC 1 cut(s) 364
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 215
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 48, 410, 456
BlsI GCNGC 3 cut(s) 49, 411, 457
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 353
BmeRI GACNNNNNGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 2 cut(s) 376, 421
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 450
Bse3DI GCAATG 2 cut(s) 48, 227
BseBI CCWGG 1 cut(s) 215
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 2 cut(s) 48, 227
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 450
BseXI GCAGC 2 cut(s) 34, 396
BshFI GGCC 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 214
BsnI GGCC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 149
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 1 cut(s) 456
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 328
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 41
BspPI GGATC 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 48, 227
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 450
BssMI GATC 1 cut(s) 259
BssNAI GTATAC 1 cut(s) 443
BssNI GRCGYC 1 cut(s) 33
Bst1107I GTATAC 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 200, 252
BstENI CCTNNNNNAGG 1 cut(s) 212
BstKTI GATC 1 cut(s) 262
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 34, 396
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 259
BstZ17I GTATAC 1 cut(s) 443
BsuRI GGCC 1 cut(s) 426
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
BveI ACCTGC 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 353
CseI GACGC 1 cut(s) 41
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 1 cut(s) 106
CviJI RGCY 9 cut(s) 47, 147, 153, 172, 292, 336, 409, 426, 455
CviKI_1 RGCY 9 cut(s) 47, 147, 153, 172, 292, 336, 409, 426, 455
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 200, 252
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
DriI GACNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 381
Eam1105I GACNNNNNGTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 147
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 149
FaiI YATR 5 cut(s) 102, 310, 312, 351, 443
FblI GTMKAC 2 cut(s) 224, 442
Fnu4HI GCNGC 3 cut(s) 48, 410, 456
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 3 cut(s) 48, 410, 456
FspBI CTAG 1 cut(s) 293
GluI GCNGC 3 cut(s) 48, 410, 456
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 1 cut(s) 426
HgaI GACGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 33
HincII GTYRAC 2 cut(s) 225, 322
HindII GTYRAC 2 cut(s) 225, 322
HinfI GANTC 1 cut(s) 111
HphI GGTGA 3 cut(s) 89, 95, 308
Hpy166II GTNNAC 4 cut(s) 106, 225, 322, 443
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 4 cut(s) 29, 244, 360, 383
Hpy8I GTNNAC 4 cut(s) 106, 225, 322, 443
HpyAV CCTTC 3 cut(s) 74, 85, 293
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4V TGCA 6 cut(s) 4, 50, 142, 232, 412, 438
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 2 cut(s) 200, 252
Hsp92I GRCGYC 1 cut(s) 33
Kzo9I GATC 1 cut(s) 259
LpnPI CCDG 9 cut(s) 36, 42, 49, 135, 171, 200, 227, 405, 431
Lsp1109I GCAGC 2 cut(s) 34, 396
LweI GCATC 2 cut(s) 376, 421
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 1 cut(s) 293
MaeIII GTNAC 1 cut(s) 391
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 3 cut(s) 26, 107, 368
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 149
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 3 cut(s) 235, 276, 428
MluNI TGGCCA 1 cut(s) 426
MnlI CCTC 9 cut(s) 50, 56, 62, 68, 79, 218, 351, 384, 390
Mox20I TGGCCA 1 cut(s) 426
MroXI GAANNNNTTC 2 cut(s) 18, 239
MscI TGGCCA 1 cut(s) 426
MseI TTAA 1 cut(s) 460
MslI CAYNNNNRTG 2 cut(s) 122, 348
Msp20I TGGCCA 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 47
NdeII GATC 1 cut(s) 259
NlaIV GGNNCC 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 122
PaqCI CACCTGC 1 cut(s) 41
PdmI GAANNNNTTC 2 cut(s) 18, 239
PfeI GAWTC 1 cut(s) 111
PkrI GCNGC 3 cut(s) 49, 411, 457
Psp124BI GAGCTC 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 1 cut(s) 353
PstI CTGCAG 1 cut(s) 52
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
RseI CAYNNNNRTG 2 cut(s) 122, 348
SacI GAGCTC 1 cut(s) 149
SalI GTCGAC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 460
SatI GCNGC 3 cut(s) 48, 410, 456
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 1 cut(s) 149
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 2 cut(s) 376, 421
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 2 cut(s) 122, 348
Sse9I AATT 3 cut(s) 235, 276, 428
SsiI CCGC 1 cut(s) 456
SspMI CTAG 1 cut(s) 293
SstI GAGCTC 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 224, 361
TasI AATT 3 cut(s) 235, 276, 428
TatI WGTACW 1 cut(s) 105
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 1 cut(s) 460
Tru9I TTAA 1 cut(s) 460
TscAI CASTG 1 cut(s) 124
TseI GCWGC 2 cut(s) 47, 409
TspDTI ATGAA 4 cut(s) 181, 185, 327, 358
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 353
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 1 cut(s) 235
XmiI GTMKAC 2 cut(s) 224, 442
XmnI GAANNNNTTC 2 cut(s) 18, 239
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.