Rorug03G0227000

Protein of unknown function (DUF952)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001955
Physical Location & Seq
Reverse (-)
20871891 .. 20874459
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0227000.1

Sequence Viewer

Length: 708 bp
ATGGAACAAGACCTCCTCCCCAATTCAATCTCCGGCCACCAAAACGACGCCGTAGAGGACGATGACGACGCACCGACTCTAAGCCTTCAAGCCTTAGAAGCTCTCAAGGAGTTCCTCACCGAGCAAAGCCAGTCCTCAAACGACACCGCCGCCGAAGCAGACTCGGCGCCGGCTTTGATCTCGGAGGACTGGCGGATGAGCCAGTTCTGGTACGACCACCACACGGCCCAAACCCTAGCCGAAGAGGTCCTCACTCTCTGCAAATCGGACTCCACTCGAGTCGCCTGCGTCGCTTGTCCCTCACTCTACGCCTATCTCAAGAGTATTGATTCTCATGTGTCTGTACAACTGCTGGAGTATGATAGAAGGTTTGAGCAATACGGGAGTGATTTCACATTTTATGATTACAACCAACCAGAAGACTTGCCTTTGGACCTGAAGCATGGTTTTGATATTGTAGTTGCTGATCCTCCTTATCTGAGTCAAGAGTGCTTGGAGAAAGTCGCTAAAACTGTTTCGTTTCTTGCACGATCAGAAAAACCTTACTTGCTCCTCCTCACAGGAGAGGTGCAGAAAGAGAGAGCAGCTGAGCTCATGGGATTGCTTCCATGTGGTTTTAGGCCTCTGCACTCCAGCAAACTAGGAAATGAGTTTCGGCTGTTCACAAGTTATGATCCTGGGACTAGATTAGGAGGGTGGGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF952

Protein Analysis

235

Amino Acids

26.44

Weight (kDa)

4.42

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 62 - 225 7.1e-56 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AciI CCGC 3 cut(s) 147, 150, 193
AclWI GGATC 2 cut(s) 461, 668
AcoI YGGCCR 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 458
AcyI GRCGYC 2 cut(s) 48, 167
AfaI GTAC 2 cut(s) 212, 345
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 2 cut(s) 27, 89
AjnI CCWGG 1 cut(s) 676
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 3 cut(s) 101, 587, 592
AluI AGCT 3 cut(s) 101, 587, 592
Alw21I GWGCWC 1 cut(s) 594
AlwI GGATC 2 cut(s) 461, 668
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 3 cut(s) 34, 225, 620
ApeKI GCWGC 1 cut(s) 584
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 3 cut(s) 226, 247, 433
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 2 cut(s) 247, 433
BanI GGYRCC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 1 cut(s) 596
BceAI ACGGC 2 cut(s) 35, 240
BcgI CGANNNNNNTGC 2 cut(s) 267, 301
BciT130I CCWGG 1 cut(s) 678
BfaI CTAG 3 cut(s) 236, 641, 684
BfoI RGCGCY 1 cut(s) 170
BisI GCNGC 2 cut(s) 150, 585
BlpI GCTNAGC 1 cut(s) 588
BlsI GCNGC 2 cut(s) 151, 586
Bme1390I CCNGG 1 cut(s) 678
Bme18I GGWCC 2 cut(s) 247, 433
BmeT110I CYCGRG 1 cut(s) 276
BmgT120I GGNCC 3 cut(s) 226, 247, 433
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 2 cut(s) 374, 616
Bpu1102I GCTNAGC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 89, 302
BsaHI GRCGYC 2 cut(s) 48, 167
BsaJI CCNNGG 1 cut(s) 677
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 130, 194, 202
BseBI CCWGG 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 677
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 2 cut(s) 470, 579
BseNI ACTGG 3 cut(s) 130, 194, 202
BseRI GAGGAG 3 cut(s) 5, 542, 545
BseXI GCAGC 1 cut(s) 596
BsgI GTGCAG 2 cut(s) 590, 611
BshFI GGCC 3 cut(s) 36, 227, 622
BshNI GGYRCC 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 2 cut(s) 33, 170
BslFI GGGAC 2 cut(s) 282, 694
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 282, 694
BsnI GGCC 3 cut(s) 36, 227, 622
BsoBI CYCGRG 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 594
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 4 cut(s) 177, 466, 530, 673
Bsp1720I GCTNAGC 1 cut(s) 588
BspACI CCGC 3 cut(s) 147, 150, 193
BspANI GGCC 3 cut(s) 36, 227, 622
BspCNI CTCAG 2 cut(s) 471, 580
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 2 cut(s) 461, 668
BspT107I GGYRCC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 3 cut(s) 130, 194, 202
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 677
BssMI GATC 4 cut(s) 177, 466, 530, 673
BssNI GRCGYC 2 cut(s) 48, 167
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 1 cut(s) 237
BstACI GRCGYC 2 cut(s) 48, 167
BstAUI TGTACA 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 171, 286
BstDEI CTNAG 4 cut(s) 80, 94, 479, 588
BstF5I GGATG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 4 cut(s) 180, 469, 533, 676
BstMBI GATC 4 cut(s) 177, 466, 530, 673
BstMWI GCNNNNNNNGC 4 cut(s) 98, 155, 164, 290
BstNI CCWGG 1 cut(s) 678
BstSCI CCNGG 1 cut(s) 676
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 1 cut(s) 426
BsuRI GGCC 3 cut(s) 36, 227, 622
BtsCI GGATG 1 cut(s) 201
Cac8I GCNNGC 2 cut(s) 171, 286
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 226, 247, 433
CseI GACGC 3 cut(s) 56, 77, 277
Csp6I GTAC 2 cut(s) 211, 344
CviAII CATG 4 cut(s) 335, 443, 595, 609
CviQI GTAC 2 cut(s) 211, 344
DdeI CTNAG 4 cut(s) 80, 94, 479, 588
DinI GGCGCC 1 cut(s) 168
DpnI GATC 4 cut(s) 179, 468, 532, 675
DpnII GATC 4 cut(s) 177, 466, 530, 673
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
EciI GGCGGA 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 592
Eco147I AGGCCT 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 594
Eco47I GGWCC 2 cut(s) 247, 433
Eco53kI GAGCTC 1 cut(s) 592
Eco57I CTGAAG 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 592
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 594
EgeI GGCGCC 1 cut(s) 168
EheI GGCGCC 1 cut(s) 168
FaeI CATG 4 cut(s) 338, 446, 598, 612
FaiI YATR 7 cut(s) 336, 360, 402, 444, 596, 610, 672
FaqI GGGAC 2 cut(s) 282, 694
FatI CATG 4 cut(s) 334, 442, 594, 608
Fnu4HI GCNGC 2 cut(s) 150, 585
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 2 cut(s) 150, 585
FspBI CTAG 3 cut(s) 236, 641, 684
GlaI GCGC 1 cut(s) 168
GluI GCNGC 2 cut(s) 150, 585
GsuI CTGGAG 2 cut(s) 374, 616
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 3 cut(s) 36, 227, 622
HapII CCGG 2 cut(s) 33, 170
HgaI GACGC 3 cut(s) 56, 77, 277
HhaI GCGC 1 cut(s) 169
Hin1I GRCGYC 2 cut(s) 48, 167
Hin1II CATG 4 cut(s) 338, 446, 598, 612
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 6 cut(s) 76, 161, 269, 279, 329, 481
HpaII CCGG 2 cut(s) 33, 170
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 663
Hpy188I TCNGA 4 cut(s) 184, 268, 480, 535
Hpy188III TCNNGA 2 cut(s) 319, 485
Hpy8I GTNNAC 1 cut(s) 663
Hpy99I CGWCG 3 cut(s) 50, 71, 293
HpyAV CCTTC 2 cut(s) 95, 360
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4V TGCA 4 cut(s) 261, 527, 571, 628
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 155, 164, 290
HpyF3I CTNAG 4 cut(s) 80, 94, 479, 588
Hsp92I GRCGYC 2 cut(s) 48, 167
Hsp92II CATG 4 cut(s) 338, 446, 598, 612
HspAI GCGC 1 cut(s) 167
KasI GGCGCC 1 cut(s) 166
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Kzo9I GATC 4 cut(s) 177, 466, 530, 673
LmnI GCTCC 1 cut(s) 555
Lsp1109I GCAGC 1 cut(s) 596
MaeI CTAG 3 cut(s) 236, 641, 684
MalI GATC 4 cut(s) 179, 468, 532, 675
MboI GATC 4 cut(s) 177, 466, 530, 673
MboII GAAGA 2 cut(s) 254, 431
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 2 cut(s) 22, 703
Mly113I GGCGCC 1 cut(s) 167
MlyI GAGTC 5 cut(s) 70, 155, 263, 288, 490
MroNI GCCGGC 1 cut(s) 169
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 2 cut(s) 33, 170
MspR9I CCNGG 1 cut(s) 678
MvaI CCWGG 1 cut(s) 678
MwoI GCNNNNNNNGC 4 cut(s) 98, 155, 164, 290
NaeI GCCGGC 1 cut(s) 171
NarI GGCGCC 1 cut(s) 167
NdeII GATC 4 cut(s) 177, 466, 530, 673
NgoMIV GCCGGC 1 cut(s) 169
NlaIII CATG 4 cut(s) 338, 446, 598, 612
NlaIV GGNNCC 1 cut(s) 168
NmeAIII GCCGAG 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 276
PceI AGGCCT 1 cut(s) 622
PdiI GCCGGC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 151, 586
PleI GAGTC 5 cut(s) 70, 155, 263, 287, 489
PluTI GGCGCC 1 cut(s) 170
PpsI GAGTC 5 cut(s) 70, 155, 263, 287, 489
PpuMI RGGWCCY 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 676
PspGI CCWGG 1 cut(s) 676
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 3 cut(s) 226, 247, 433
PspPPI RGGWCCY 1 cut(s) 247
PspXI VCTCGAGB 1 cut(s) 276
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 2 cut(s) 212, 345
RsaNI GTAC 2 cut(s) 211, 344
SacI GAGCTC 1 cut(s) 594
SatI GCNGC 2 cut(s) 150, 585
Sau3AI GATC 4 cut(s) 177, 466, 530, 673
Sau96I GGNCC 3 cut(s) 226, 247, 433
SchI GAGTC 5 cut(s) 70, 155, 263, 288, 490
ScrFI CCNGG 1 cut(s) 678
SduI GDGCHC 1 cut(s) 594
SetI ASST 9 cut(s) 15, 103, 249, 371, 438, 544, 570, 589, 594
SfoI GGCGCC 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 276
SinI GGWCC 2 cut(s) 247, 433
SlaI CTCGAG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 104, 276, 317
SmoI CTYRAG 3 cut(s) 104, 276, 317
Sse9I AATT 2 cut(s) 22, 703
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 3 cut(s) 147, 150, 193
SspDI GGCGCC 1 cut(s) 166
SspMI CTAG 3 cut(s) 236, 641, 684
SstI GAGCTC 1 cut(s) 594
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 1 cut(s) 676
TaaI ACNGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 277
TasI AATT 2 cut(s) 22, 703
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 329
TseI GCWGC 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 247, 433
XhoI CTCGAG 1 cut(s) 276
XspI CTAG 3 cut(s) 236, 641, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.