Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0001955

Physical Location & Seq

Genomic Coordinates Forward (+)

32680567 .. 32685473

Gene Structure 4907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG311000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGGAGGAGACATTTGTGCCTTTTCGCGGGATCAAGAATGACCTTAGAGGAAGGTTGATGTGCTACAAGCAAGATTGGACTGGTGGCTTCAGAGCAGGATACAGGATTTTGGCTCCCACCACATACATCTTCTTTGCTTCAGCAATCCCGGTCATTTCATTTGGTGAACAGCTGGAGCGAAGTACTGATGGAGTTTTGACAGCAGTGCAAACCTTGGTATCTACGGCTGTCTGTGGAATCATACACTCCATAGTTGGTGGTCAACCCTTGCTGATATTGGGTGTGGCTGAGCCTACTGTAATCATGTACACCTTTATGTTTAATTTTGCCAAGGAGCAAAAAGACTTAGGTCCAAAGCTGTTTCTAGCATGGACAGGATGGGTATGTGTATGGACAGCAGTGTTGCTGTTCTTATTGGCTATTTTGGGTGCTTGTTCCATTATAAACAGATTCACCCGTGTAGCTGGAGAACTGTTTGGCCTCCTTATTGCTATGCTCTTCATGCAGCAATCCATTAAAGGAATTGTGGAGGAGTTTCGCATACCACAAAGAGAAGATACAAGTTTAGCAGAGTTTCTTCCTTCATGGAGGTTCGCAAATGGGATGTTTGCTTTGGTATTGTCATTTGGCCTTCTTCTCACTGCATTAAAAAGTCGGAAAGCAAGGTCTTGGCGCTATGGCTCTGGTTCGCTTAGAGGCTTCATAGCTGATTATGGAGTGCCACTTATGGTTCTAGTCTGGACTGCTGTATCTTACATACCAACTAGTAGTGTTCCGAAAGGAATCCCACGACGGCTCTTTAGTCCAAATCCATGGTCACCTGGTGCATATGAGAATTGGACTGTCATTAAGCAAATGCTCGATGTTCCGGTCCTTTATATCATTGGAGCTTTCATTCCAGCAACAATGATTGCAGTGCTTTATTATTTCGACCACAGTGTAGCATCCCAACTTGCTCAGCAGAAAGAGTTCAATTTGAGAAAGCCATCTTCTTACCATTATGACTTGCTTCTTTTGGGGTTTCTGACCTTAATGTGTGGTCTGCTAGGAATTCCGCCATCAAATGGTGTCATTCCACAGTCACCAATGCATACTAAGAGTTTGGCTACTCTTAAACACCAGTTGCTTCGTAATAAACTTGTAGCAACAGCACGCAAAAGTATGAGAAAGAATGCTAGCTTGGGACAGCTGTATGGAAATATGCAAGAAGCCTATAATCAAATGCAGACTCCTTTGATTTACCAGGGTGCCTCATCTCAGGGACTGAATGAGTTAAAAGAATCAACTATCCAGGCAGCTTCAAGTATGGGAAACTATATTGATGCGCCAGTTGATGATACAGTGTTCGATGTGGAGAAAGAGATTGACGATCTATTACCTGTTGAGGTGAAAGAACAGCGTCTTAGTAACATACTTCAAGCTATACTGGTCGGAGCATGTGTTGCAGCCATGCCTATACTAAAAAAGATCCCAACCTCTGTCCTTTGGGGCTACTTTGCCTTCATGGCGATTGAAAGCTTACCCGGTAACCAATTTTGGGAAAGGATCTTACTGCTCTTTACTGCTCCCAGTAGAAGATTCAAAGTCCTCGAGGAGTACCATGCAACTTTTGTAGAAACTGTGCCTTTCAAGACAATTGTGATGTTTACAATTTTCCAAACTGTATACTTGCTTATATGTTTTGGGCTTACATGGGTTCCAATTGCCGGGGTCATGTTTCCATTGATGATCATGCTGTTGGTTCCTGTGAGACAATACTTTCTGCCCAAGTTTTTCAAAGGAGCTCATCTTCAAGATTTGGATGCAGCAGAGTATGAAGAGGCTCCAGCTTTACCATTTAATCTTGCAGCGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006873 GO:0006885 GO:0007275 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0022622 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0071944 GO:0098771 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

618

Amino Acids

69.15

Weight (kDa)

8.84

Isoelectric Point (pI)

37.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	7 - 180	1.4e-34	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	200 - 372	8.2e-25	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	461 - 603	1.9e-30	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47160 AT2G47160 AT3G62270
fragaria_vesca	FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400
malus_domestica	MD06G1173100.v1.1 MD09G1287700.v1.1 MD14G1179400.v1.1 MD17G1279900.v1.1
prunus_persica	Prupe.3G005600_v2.0.a1 Prupe.5G172600_v2.0.a1
pyrus_communis	pycom06g15420 pycom14g14930 pycom14g14940
rosa_chinensis	RchiOBHm_Chr3g0486761 RchiOBHm_Chr7g0193431
rosa_laevigata	RLG00000004268 RLG00000023062
rosa_multiflora	Rmu_sc0006629.1_g000001 Rmu_sc0012783.1_g000009 Rmu_sc0013001.1_g000011
rosa_roxburghii	Rroxscaffold_3G00262090 Rroxscaffold_6G00395610
rosa_rugosa	Rorug03G0227000 Rorug03G0227100 Rorug07G0009500
rosa_samantha	Rh3AG276900 Rh3BG311800 Rh3CG311000 Rh3DG307500 Rh7AG136100 Rh7BG136500 Rh7CG140000 Rh7DG137800
rosa_wichuraiana	Rw3G024620 Rw7G011610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	443
AbsI	CCTCGAGG	1 cut(s)	1589
AccB1I	GGYRCC	1 cut(s)	1247
AccB7I	CCANNNNNTGG	1 cut(s)	1064
AccI	GTMKAC	1 cut(s)	1665
AccII	CGCG	1 cut(s)	27
AciI	CCGC	3 cut(s)	27, 1055, 1850
AclWI	GGATC	3 cut(s)	38, 1462, 1553
AcsI	RAATTY	1 cut(s)	1050
AcuI	CTGAAG	2 cut(s)	73, 123
AdeI	CACNNNGTG	1 cut(s)	824
AfaI	GTAC	3 cut(s)	184, 308, 1598
AfiI	CCNNNNNNNGG	4 cut(s)	26, 1064, 1537, 1706
AgsI	TTSAA	8 cut(s)	973, 1302, 1418, 1514, 1582, 1630, 1777, 1793
AhlI	ACTAGT	1 cut(s)	764
AjnI	CCWGG	3 cut(s)	820, 1242, 1290
AjuI	GAANNNNNNNTTGG	2 cut(s)	1163, 1195
Alw21I	GWGCWC	1 cut(s)	1786
Alw26I	GTCTC	2 cut(s)	2, 1744
AlwI	GGATC	3 cut(s)	38, 1462, 1553
AlwNI	CAGNNNCTG	1 cut(s)	1264
Ama87I	CYCGRG	1 cut(s)	1589
AoxI	GGCC	2 cut(s)	478, 628
ApeKI	GCWGC	5 cut(s)	505, 1295, 1445, 1805, 1847
ApoI	RAATTY	1 cut(s)	1050
ArsI	GACNNNNNNTTYG	2 cut(s)	800, 832
Asp700I	GAANNNNTTC	1 cut(s)	968
AspLEI	GCGC	2 cut(s)	675, 1327
AspS9I	GGNCC	2 cut(s)	350, 871
AsuC2I	CCSGG	3 cut(s)	149, 1524, 1708
AsuHPI	GGTGA	5 cut(s)	176, 445, 810, 1074, 1399
AsuNHI	GCTAGC	1 cut(s)	1175
AvaI	CYCGRG	1 cut(s)	1589
AvaII	GGWCC	2 cut(s)	350, 871
BaeI	ACNNNNGTAYC	4 cut(s)	201, 234, 732, 765
BanI	GGYRCC	1 cut(s)	1247
BanII	GRGCYC	1 cut(s)	1786
Bbv12I	GWGCWC	1 cut(s)	1786
BbvI	GCAGC	4 cut(s)	517, 1307, 1457, 1817
BccI	CCATC	4 cut(s)	182, 372, 994, 1066
BceAI	ACGGC	2 cut(s)	240, 809
BciT130I	CCWGG	3 cut(s)	822, 1244, 1292
BciVI	GTATCC	1 cut(s)	92
BclI	TGATCA	1 cut(s)	1728
BcnI	CCSGG	3 cut(s)	149, 1524, 1708
BcoDI	GTCTC	2 cut(s)	2, 1744
BcuI	ACTAGT	1 cut(s)	764
BfaI	CTAG	5 cut(s)	365, 734, 765, 1046, 1176
BfoI	RGCGCY	1 cut(s)	676
BfuI	GTATCC	1 cut(s)	92
BglI	GCCNNNNNGGC	1 cut(s)	1505
BisI	GCNGC	5 cut(s)	506, 1296, 1446, 1806, 1848
BlpI	GCTNAGC	2 cut(s)	288, 957
BlsI	GCNGC	5 cut(s)	507, 1297, 1447, 1807, 1849
BmcAI	AGTACT	1 cut(s)	184
Bme1390I	CCNGG	6 cut(s)	149, 822, 1244, 1292, 1524, 1708
Bme18I	GGWCC	2 cut(s)	350, 871
BmeT110I	CYCGRG	1 cut(s)	1589
BmgT120I	GGNCC	2 cut(s)	350, 871
BmiI	GGNNCC	5 cut(s)	114, 1249, 1698, 1743, 1824
BmrFI	CCNGG	6 cut(s)	149, 822, 1244, 1292, 1524, 1708
BmrI	ACTGGG	1 cut(s)	1563
BmsI	GCATC	3 cut(s)	953, 1312, 1792
BmtI	GCTAGC	1 cut(s)	1179
BmuI	ACTGGG	1 cut(s)	1563
BoxI	GACNNNNGTC	1 cut(s)	348
BpmI	CTGGAG	3 cut(s)	194, 486, 1809
Bpu1102I	GCTNAGC	2 cut(s)	288, 957
BpuMI	CCSGG	3 cut(s)	149, 1524, 1708
BsaJI	CCNNGG	5 cut(s)	213, 330, 812, 1243, 1707
BsaWI	WCCGGW	1 cut(s)	868
BsaXI	ACNNNNNCTCC	2 cut(s)	1425, 1455
Bsc4I	CCNNNNNNNGG	4 cut(s)	26, 1064, 1537, 1706
Bse1I	ACTGG	5 cut(s)	85, 1120, 1328, 1431, 1569
BseBI	CCWGG	3 cut(s)	822, 1244, 1292
BseDI	CCNNGG	5 cut(s)	213, 330, 812, 1243, 1707
BseGI	GGATG	4 cut(s)	383, 609, 944, 1807
BseLI	CCNNNNNNNGG	4 cut(s)	26, 1064, 1537, 1706
BseMII	CTCAG	3 cut(s)	279, 971, 1271
BseNI	ACTGG	5 cut(s)	85, 1120, 1328, 1431, 1569
BseRI	GAGGAG	3 cut(s)	20, 545, 1607
BseXI	GCAGC	4 cut(s)	517, 1307, 1457, 1817
Bsh1236I	CGCG	1 cut(s)	27
BshFI	GGCC	2 cut(s)	480, 630
BshNI	GGYRCC	1 cut(s)	1247
BsiHKAI	GWGCWC	1 cut(s)	1786
BsiHKCI	CYCGRG	1 cut(s)	1589
BsiSI	CCGG	4 cut(s)	149, 869, 1524, 1707
BslFI	GGGAC	2 cut(s)	1197, 1275
BslI	CCNNNNNNNGG	4 cut(s)	26, 1064, 1537, 1706
BsmAI	GTCTC	2 cut(s)	2, 1744
BsmFI	GGGAC	2 cut(s)	1197, 1275
BsmI	GAATGC	1 cut(s)	1177
BsnI	GGCC	2 cut(s)	480, 630
BsoBI	CYCGRG	1 cut(s)	1589
Bsp1286I	GDGCHC	1 cut(s)	1786
Bsp1407I	TGTACA	1 cut(s)	306
Bsp143I	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
Bsp1720I	GCTNAGC	2 cut(s)	288, 957
Bsp19I	CCATGG	1 cut(s)	812
BspACI	CCGC	3 cut(s)	27, 1055, 1850
BspANI	GGCC	2 cut(s)	480, 630
BspCNI	CTCAG	3 cut(s)	280, 970, 1270
BspFNI	CGCG	1 cut(s)	27
BspLI	GGNNCC	5 cut(s)	114, 1249, 1698, 1743, 1824
BspOI	GCTAGC	1 cut(s)	1179
BspPI	GGATC	3 cut(s)	38, 1462, 1553
BspQI	GCTCTTC	1 cut(s)	503
BspT107I	GGYRCC	1 cut(s)	1247
BsrGI	TGTACA	1 cut(s)	306
BsrI	ACTGG	5 cut(s)	85, 1120, 1328, 1431, 1569
BssECI	CCNNGG	5 cut(s)	213, 330, 812, 1243, 1707
BssMI	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
BssNAI	GTATAC	1 cut(s)	1666
BssT1I	CCWWGG	3 cut(s)	213, 330, 812
Bst1107I	GTATAC	1 cut(s)	1666
Bst2UI	CCWGG	3 cut(s)	822, 1244, 1292
Bst4CI	ACNGT	8 cut(s)	298, 474, 844, 938, 1080, 1342, 1621, 1663
Bst6I	CTCTTC	2 cut(s)	503, 1812
BstAPI	GCANNNNNTGC	1 cut(s)	1442
BstAUI	TGTACA	1 cut(s)	306
BstC8I	GCNNGC	2 cut(s)	1153, 1177
BstDEI	CTNAG	8 cut(s)	44, 288, 346, 692, 957, 1095, 1257, 1403
BstDSI	CCRYGG	1 cut(s)	812
BstEII	GGTNACC	2 cut(s)	816, 1526
BstF5I	GGATG	4 cut(s)	383, 609, 944, 1807
BstFNI	CGCG	1 cut(s)	27
BstH2I	RGCGCY	1 cut(s)	676
BstHHI	GCGC	2 cut(s)	675, 1327
BstKTI	GATC	5 cut(s)	33, 1372, 1470, 1548, 1731
BstMAI	GTCTC	2 cut(s)	2, 1744
BstMBI	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
BstMWI	GCNNNNNNNGC	3 cut(s)	502, 1442, 1505
BstNI	CCWGG	3 cut(s)	822, 1244, 1292
BstNSI	RCATGY	1 cut(s)	1440
BstPAI	GACNNNNGTC	1 cut(s)	348
BstPI	GGTNACC	2 cut(s)	816, 1526
BstSCI	CCNGG	6 cut(s)	147, 820, 1242, 1290, 1522, 1706
BstUI	CGCG	1 cut(s)	27
BstV1I	GCAGC	4 cut(s)	517, 1307, 1457, 1817
BstX2I	RGATCY	2 cut(s)	1467, 1545
BstXI	CCANNNNNNTGG	1 cut(s)	813
BstYI	RGATCY	2 cut(s)	1467, 1545
BstZ17I	GTATAC	1 cut(s)	1666
BsuI	GTATCC	1 cut(s)	92
BsuRI	GGCC	2 cut(s)	480, 630
BtgI	CCRYGG	1 cut(s)	812
BtsCI	GGATG	4 cut(s)	383, 609, 944, 1807
BtsI	GCAGTG	4 cut(s)	210, 405, 639, 921
BtsIMutI	CAGTG	6 cut(s)	210, 405, 639, 921, 943, 1347
Cac8I	GCNNGC	2 cut(s)	1153, 1177
CaiI	CAGNNNCTG	1 cut(s)	1264
CfoI	GCGC	2 cut(s)	675, 1327
Cfr13I	GGNCC	2 cut(s)	350, 871
CseI	GACGC	1 cut(s)	1388
CsiI	ACCWGGT	1 cut(s)	820
Csp6I	GTAC	3 cut(s)	183, 307, 1597
CviQI	GTAC	3 cut(s)	183, 307, 1597
DdeI	CTNAG	8 cut(s)	44, 288, 346, 692, 957, 1095, 1257, 1403
DpnI	GATC	5 cut(s)	32, 1371, 1469, 1547, 1730
DpnII	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
DraIII	CACNNNGTG	1 cut(s)	824
Eam1104I	CTCTTC	2 cut(s)	503, 1812
EarI	CTCTTC	2 cut(s)	503, 1812
EciI	GGCGGA	1 cut(s)	1044
Ecl136II	GAGCTC	1 cut(s)	1784
Eco130I	CCWWGG	3 cut(s)	213, 330, 812
Eco24I	GRGCYC	1 cut(s)	1786
Eco47I	GGWCC	2 cut(s)	350, 871
Eco53kI	GAGCTC	1 cut(s)	1784
Eco57I	CTGAAG	2 cut(s)	73, 123
Eco88I	CYCGRG	1 cut(s)	1589
Eco91I	GGTNACC	2 cut(s)	816, 1526
EcoICRI	GAGCTC	1 cut(s)	1784
EcoO65I	GGTNACC	2 cut(s)	816, 1526
EcoRI	GAATTC	1 cut(s)	1050
EcoRII	CCWGG	3 cut(s)	820, 1242, 1290
EcoT14I	CCWWGG	3 cut(s)	213, 330, 812
EcoT22I	ATGCAT	1 cut(s)	1092
EcoT38I	GRGCYC	1 cut(s)	1786
ErhI	CCWWGG	3 cut(s)	213, 330, 812
FaqI	GGGAC	2 cut(s)	1197, 1275
FauI	CCCGC	1 cut(s)	20
FauNDI	CATATG	1 cut(s)	829
FbaI	TGATCA	1 cut(s)	1728
FblI	GTMKAC	1 cut(s)	1665
Fnu4HI	GCNGC	5 cut(s)	506, 1296, 1446, 1806, 1848
FokI	GGATG	4 cut(s)	390, 616, 931, 1814
FriOI	GRGCYC	1 cut(s)	1786
Fsp4HI	GCNGC	5 cut(s)	506, 1296, 1446, 1806, 1848
FspBI	CTAG	5 cut(s)	365, 734, 765, 1046, 1176
GlaI	GCGC	2 cut(s)	674, 1326
GluI	GCNGC	5 cut(s)	506, 1296, 1446, 1806, 1848
GsuI	CTGGAG	3 cut(s)	194, 486, 1809
HaeII	RGCGCY	1 cut(s)	676
HaeIII	GGCC	2 cut(s)	480, 630
HapII	CCGG	4 cut(s)	149, 869, 1524, 1707
HgaI	GACGC	1 cut(s)	1388
HhaI	GCGC	2 cut(s)	675, 1327
Hin6I	GCGC	2 cut(s)	673, 1325
HinP1I	GCGC	2 cut(s)	673, 1325
HincII	GTYRAC	1 cut(s)	263
HindII	GTYRAC	1 cut(s)	263
HindIII	AAGCTT	1 cut(s)	1516
HinfI	GANTC	6 cut(s)	237, 450, 783, 1228, 1280, 1578
HpaII	CCGG	4 cut(s)	149, 869, 1524, 1707
HphI	GGTGA	5 cut(s)	176, 445, 810, 1074, 1399
Hpy166II	GTNNAC	5 cut(s)	167, 263, 309, 1647, 1666
Hpy188I	TCNGA	5 cut(s)	92, 657, 777, 1026, 1433
Hpy188III	TCNNGA	4 cut(s)	34, 739, 1630, 1793
Hpy8I	GTNNAC	5 cut(s)	167, 263, 309, 1647, 1666
Hpy99I	CGWCG	1 cut(s)	795
HpyAV	CCTTC	4 cut(s)	45, 591, 641, 1510
HpyCH4III	ACNGT	8 cut(s)	298, 474, 844, 938, 1080, 1342, 1621, 1663
HpyF10VI	GCNNNNNNNGC	3 cut(s)	502, 1442, 1505
HpyF3I	CTNAG	8 cut(s)	44, 288, 346, 692, 957, 1095, 1257, 1403
HspAI	GCGC	2 cut(s)	673, 1325
Ksp22I	TGATCA	1 cut(s)	1728
Kzo9I	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
LguI	GCTCTTC	1 cut(s)	503
LmnI	GCTCC	8 cut(s)	118, 175, 334, 887, 1433, 1570, 1781, 1828
Lsp1109I	GCAGC	4 cut(s)	517, 1307, 1457, 1817
LweI	GCATC	3 cut(s)	953, 1312, 1792
MabI	ACCWGGT	1 cut(s)	820
MaeI	CTAG	5 cut(s)	365, 734, 765, 1046, 1176
MaeIII	GTNAC	4 cut(s)	816, 1080, 1406, 1526
MalI	GATC	5 cut(s)	32, 1371, 1469, 1547, 1730
MboI	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
MboII	GAAGA	9 cut(s)	121, 490, 566, 569, 626, 981, 1587, 1781, 1829
MfeI	CAATTG	2 cut(s)	1635, 1701
MflI	RGATCY	2 cut(s)	1467, 1545
MhlI	GDGCHC	1 cut(s)	1786
MluCI	AATT	9 cut(s)	322, 522, 835, 973, 1050, 1532, 1635, 1650, 1701
MlyI	GAGTC	1 cut(s)	1222
MmeI	TCCRAC	2 cut(s)	635, 1411
Mph1103I	ATGCAT	1 cut(s)	1092
MroXI	GAANNNNTTC	1 cut(s)	968
MseI	TTAA	8 cut(s)	321, 516, 647, 849, 1031, 1113, 1274, 1839
MslI	CAYNNNNRTG	1 cut(s)	314
MspA1I	CMGCKG	3 cut(s)	172, 1189, 1850
MspI	CCGG	4 cut(s)	149, 869, 1524, 1707
MspR9I	CCNGG	6 cut(s)	149, 822, 1244, 1292, 1524, 1708
MunI	CAATTG	2 cut(s)	1635, 1701
Mva1269I	GAATGC	1 cut(s)	1177
MvaI	CCWGG	3 cut(s)	822, 1244, 1292
MvnI	CGCG	1 cut(s)	27
MwoI	GCNNNNNNNGC	3 cut(s)	502, 1442, 1505
NciI	CCSGG	3 cut(s)	149, 1524, 1708
NcoI	CCATGG	1 cut(s)	812
NdeI	CATATG	1 cut(s)	829
NdeII	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
NheI	GCTAGC	1 cut(s)	1175
NlaIV	GGNNCC	5 cut(s)	114, 1249, 1698, 1743, 1824
NmuCI	GTSAC	2 cut(s)	816, 1080
NsiI	ATGCAT	1 cut(s)	1092
NspI	RCATGY	1 cut(s)	1440
PaeR7I	CTCGAG	1 cut(s)	1589
PciSI	GCTCTTC	1 cut(s)	503
PctI	GAATGC	1 cut(s)	1177
PdmI	GAANNNNTTC	1 cut(s)	968
PfeI	GAWTC	5 cut(s)	237, 450, 783, 1280, 1578
PflMI	CCANNNNNTGG	1 cut(s)	1064
PkrI	GCNGC	5 cut(s)	507, 1297, 1447, 1807, 1849
PleI	GAGTC	1 cut(s)	1222
PpsI	GAGTC	1 cut(s)	1222
PshAI	GACNNNNGTC	1 cut(s)	348
PsiI	TTATAA	1 cut(s)	443
Psp124BI	GAGCTC	1 cut(s)	1786
Psp6I	CCWGG	3 cut(s)	820, 1242, 1290
PspEI	GGTNACC	2 cut(s)	816, 1526
PspGI	CCWGG	3 cut(s)	820, 1242, 1290
PspN4I	GGNNCC	5 cut(s)	114, 1249, 1698, 1743, 1824
PspPI	GGNCC	2 cut(s)	350, 871
PspXI	VCTCGAGB	1 cut(s)	1589
PstNI	CAGNNNCTG	1 cut(s)	1264
PsuI	RGATCY	2 cut(s)	1467, 1545
PvuII	CAGCTG	2 cut(s)	172, 1189
RsaI	GTAC	3 cut(s)	184, 308, 1598
RsaNI	GTAC	3 cut(s)	183, 307, 1597
RseI	CAYNNNNRTG	1 cut(s)	314
SacI	GAGCTC	1 cut(s)	1786
SapI	GCTCTTC	1 cut(s)	503
SaqAI	TTAA	8 cut(s)	321, 516, 647, 849, 1031, 1113, 1274, 1839
SatI	GCNGC	5 cut(s)	506, 1296, 1446, 1806, 1848
Sau3AI	GATC	5 cut(s)	30, 1369, 1467, 1545, 1728
Sau96I	GGNCC	2 cut(s)	350, 871
ScaI	AGTACT	1 cut(s)	184
SchI	GAGTC	1 cut(s)	1222
ScrFI	CCNGG	6 cut(s)	149, 822, 1244, 1292, 1524, 1708
SduI	GDGCHC	1 cut(s)	1786
SexAI	ACCWGGT	1 cut(s)	820
SfaNI	GCATC	3 cut(s)	953, 1312, 1792
Sfr274I	CTCGAG	1 cut(s)	1589
SinI	GGWCC	2 cut(s)	350, 871
SlaI	CTCGAG	1 cut(s)	1589
SmiMI	CAYNNNNRTG	1 cut(s)	314
SmlI	CTYRAG	1 cut(s)	1589
SmoI	CTYRAG	1 cut(s)	1589
SpeI	ACTAGT	1 cut(s)	764
Sse9I	AATT	9 cut(s)	322, 522, 835, 973, 1050, 1532, 1635, 1650, 1701
SsiI	CCGC	3 cut(s)	27, 1055, 1850
SspMI	CTAG	5 cut(s)	365, 734, 765, 1046, 1176
SstI	GAGCTC	1 cut(s)	1786
StyD4I	CCNGG	6 cut(s)	147, 820, 1242, 1290, 1522, 1706
StyI	CCWWGG	3 cut(s)	213, 330, 812
TaaI	ACNGT	8 cut(s)	298, 474, 844, 938, 1080, 1342, 1621, 1663
TaqI	TCGA	4 cut(s)	861, 930, 1347, 1590
TasI	AATT	9 cut(s)	322, 522, 835, 973, 1050, 1532, 1635, 1650, 1701
TatI	WGTACW	2 cut(s)	182, 306
TfiI	GAWTC	5 cut(s)	237, 450, 783, 1280, 1578
Tru1I	TTAA	8 cut(s)	321, 516, 647, 849, 1031, 1113, 1274, 1839
Tru9I	TTAA	8 cut(s)	321, 516, 647, 849, 1031, 1113, 1274, 1839
TscAI	CASTG	6 cut(s)	210, 405, 646, 921, 943, 1347
TseFI	GTSAC	2 cut(s)	816, 1080
TseI	GCWGC	5 cut(s)	505, 1295, 1445, 1805, 1847
Tsp45I	GTSAC	2 cut(s)	816, 1080
TspDTI	ATGAA	7 cut(s)	147, 490, 573, 691, 883, 1492, 1830
TspRI	CASTG	6 cut(s)	210, 405, 646, 921, 943, 1347
Van91I	CCANNNNNTGG	1 cut(s)	1064
VpaK11BI	GGWCC	2 cut(s)	350, 871
XapI	RAATTY	1 cut(s)	1050
XceI	RCATGY	1 cut(s)	1440
XhoI	CTCGAG	1 cut(s)	1589
XmiI	GTMKAC	1 cut(s)	1665
XmnI	GAANNNNTTC	1 cut(s)	968
XspI	CTAG	5 cut(s)	365, 734, 765, 1046, 1176
ZrmI	AGTACT	1 cut(s)	184
Zsp2I	ATGCAT	1 cut(s)	1092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.