Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001955

Physical Location & Seq

Genomic Coordinates Reverse (-)

27204004 .. 27209504

Gene Structure 5501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1179400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGGAAGAAACATTTGTTCCCTTTCGCGGGATAAAGAATGACCTCAAGGGGAGACTTCTGTGCTACAAACAGGATTGGACTGGTGGTTTACGCGCCGGAATCAGGATCTTGGCGCCAACAACATACATATTCTTTGCTTCAGCAATTCCTGTTATCTCATTTGGGGAACAGCTCGAAAGAAATACAAATGGAAGCTTGACTGCAGTACAGACTCTTGCATCCACATCACTTTGTGGCATAATCCACTCGATTGTTGGAGGCCAGCCCCTGCTTATACTAGGGGTGGCTGAGCCAACGGTATTGATGTATACATTTATGTTCAACTTTGCCAAGGACCGGAAGGATTTGGGGCAGAAACTCTTCTTAGCCTGGACTGGATGGGTATGTATGTGGACAGCCCTTTTGCTCTTCTTGCTGGCGGTTCTGGGTGCATGCTCTATTATCAATAGGTTTACGCGTGTTGCTGGTGAATTGTTTGGTCTGCTAATTGCAATGCTTTTTATGCAGCAGGCCATAAAGGGACTCGTGGAGGAATTTGGCATACCCCAGAGAGAAAATCCCAATCAGACTGCACTGCAACCTTCTTGGCGGTTTGGCAATGGAATGTTTGCGCTAGTTCTGTCATTTGGCCTTCTTTTAACTGCATTGAGAAGCCGTAAAGCTAGATCCTGGCGTTATGGCACAGGTTGGCTACGGGGATTAATTGCGGATTATGGAGTTCCATTTATGGTGCTTGTTTGGACTGCGGCCTCTTACATACCAGTTAATGATGTCCCTAGAGGAATCCCAAGACGACTGTTCAGTCCAAACCCATGGTCTCCTGGTGCATACTCAAATTGGACAGTTATCAAGGAGATGATGAATGTACCTCCATTATATATAGTTGGAGCATTTATACCAGCAACTATGATCGCTGTGCTCTACTACTTTGATCACAGTGTTGCATCTCAACTTGCCCAACAGAAAGAATTTAATCTGAAGAAACCGGCTTCATATCATTATGACCTTCTCCTTCTGGGTTTTCTGGTCATGTTATGTGGGCTTATTGGCATTCCCCCTTCCAATGGAGTAATCCCACAATCTCCTATGCATACAAAAAGCTTAGCTACTCTAAAACATCAGCTTTTGCGGAATAAGCTTGCATCAACAGCTCGAAGTAGTATACGCAAAAATTCAAGTTTGAGTGAATTATACCAGAGCATGAAAGATGCATACAACGGAATGCAGACTCCACTAGTCTACCAACTTCCATCTGCTTTGGGACTAAAAGAGCTGAAAGAATCCACACTCCAATTGGCCTCTAGTGCTGGCTACATCGATGCCCCTGTTGATGAGACTGTTTTTGATGTGGATAAGGATATTGACGATCTTCTGCCCATTGAAGTAAAAGAGCAACGTCTCAGCAATCTGCTTCAATCATTGATGGCTGGTTGTTGTGTAGGTGCTATGCCTGTTTTGAAAAAAATCCCAACATCAGTTCTTTGGGGTTATTTTGCTTTCATGGCGATTGAAAGCTTGCCTGGAAATCAATTTTGGGAGAGAATATTGTTTCTTTTCACTGCTCCAAGTCGAAGATACAAGGTGCTGGAGAAGAATCATGGAACCTTTCTTGAGACAGTGCCTTTCAAAACAATTGTGACCTTCACATTGTTCCAGACGGCTTACTTGCTTATGTGTTTTGGCTTAACATGGATCCCGATTGCTGGGGTGCTATTCCCATTGTTGATCATGCTTCTAGTCCCAGTGCGGCAGTATTTTCTCCCTAAGTTTTTCAAAGGTGCTCACCTTCAAGACCTGGATGCTGCAGAATATGAGGAAGCCCCTTCCATCACTTTCAACCTGCCCCTTGAAGATCTGGATCTTCAATCAAGAACCACTAACATAGACGGTGGGGAGATGCTTGATGAAATTATCACAAGAAGCCGCGGTGAGATCCGCCACACTCATAGTTCAAAAACATCAAGTTTGAACCCTACTCCGCTAGAAGGTAGCCCAAGGATGTCACAGATAGTGCACTGCCCCCGTTTAAATGAACTGAGAGGAGAAAGAAGCCCCCGACTGAGTGCACAGGGACTTGAAACAACACAGGCTTCCAGCCCTGGGCCATCTACCCTCGGACAAATCTCTCGTGGTTCTTCAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006873 GO:0006885 GO:0007275 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0022622 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0071944 GO:0098771 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

715

Amino Acids

79.45

Weight (kDa)

9.06

Isoelectric Point (pI)

41.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	7 - 180	5.1e-35	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	202 - 373	3.9e-23	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	460 - 602	1.9e-28	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47160 AT2G47160 AT3G62270
fragaria_vesca	FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400
malus_domestica	MD06G1173100.v1.1 MD09G1287700.v1.1 MD14G1179400.v1.1 MD17G1279900.v1.1
prunus_persica	Prupe.3G005600_v2.0.a1 Prupe.5G172600_v2.0.a1
pyrus_communis	pycom06g15420 pycom14g14930 pycom14g14940
rosa_chinensis	RchiOBHm_Chr3g0486761 RchiOBHm_Chr7g0193431
rosa_laevigata	RLG00000004268 RLG00000023062
rosa_multiflora	Rmu_sc0006629.1_g000001 Rmu_sc0012783.1_g000009 Rmu_sc0013001.1_g000011
rosa_roxburghii	Rroxscaffold_3G00262090 Rroxscaffold_6G00395610
rosa_rugosa	Rorug03G0227000 Rorug03G0227100 Rorug07G0009500
rosa_samantha	Rh3AG276900 Rh3BG311800 Rh3CG311000 Rh3DG307500 Rh7AG136100 Rh7BG136500 Rh7CG140000 Rh7DG137800
rosa_wichuraiana	Rw3G024620 Rw7G011610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	801
Acc36I	ACCTGC	1 cut(s)	1848
AccB1I	GGYRCC	1 cut(s)	112
AccI	GTMKAC	3 cut(s)	308, 1162, 1239
AccII	CGCG	4 cut(s)	27, 93, 457, 1926
AclWI	GGATC	6 cut(s)	113, 660, 1687, 1700, 1866, 1927
AcsI	RAATTY	3 cut(s)	533, 968, 1171
AcuI	CTGAAG	2 cut(s)	123, 998
AcyI	GRCGYC	1 cut(s)	113
AdeI	CACNNNGTG	1 cut(s)	233
AfaI	GTAC	2 cut(s)	207, 867
AfiI	CCNNNNNNNGG	8 cut(s)	26, 27, 102, 336, 1064, 1703, 1985, 2100
AflIII	ACRYGT	1 cut(s)	455
AhlI	ACTAGT	1 cut(s)	1234
AjnI	CCWGG	6 cut(s)	368, 668, 820, 1519, 1794, 2098
AjuI	GAANNNNNNNTTGG	2 cut(s)	1516, 1548
Alw21I	GWGCWC	4 cut(s)	921, 1783, 2016, 2068
Alw26I	GTCTC	5 cut(s)	46, 822, 1328, 1403, 1607
Alw44I	GTGCAC	2 cut(s)	2012, 2064
AlwI	GGATC	6 cut(s)	113, 660, 1687, 1700, 1866, 1927
AlwNI	CAGNNNCTG	1 cut(s)	268
AoxI	GGCC	6 cut(s)	259, 510, 628, 747, 1296, 2102
ApaLI	GTGCAC	2 cut(s)	2012, 2064
ApeKI	GCWGC	2 cut(s)	505, 1802
ApoI	RAATTY	3 cut(s)	533, 968, 1171
ArsI	GACNNNNNNTTYG	4 cut(s)	385, 417, 800, 832
AseI	ATTAAT	1 cut(s)	701
Asp700I	GAANNNNTTC	1 cut(s)	359
AspLEI	GCGC	3 cut(s)	95, 115, 613
AspS9I	GGNCC	2 cut(s)	334, 2102
AsuHPI	GGTGA	3 cut(s)	479, 1775, 1940
AvaII	GGWCC	1 cut(s)	334
BaeGI	GKGCMC	2 cut(s)	2016, 2068
BamHI	GGATCC	1 cut(s)	1692
BanI	GGYRCC	1 cut(s)	112
BauI	CACGAG	2 cut(s)	524, 2127
Bbv12I	GWGCWC	4 cut(s)	921, 1783, 2016, 2068
BbvI	GCAGC	2 cut(s)	517, 1789
BccI	CCATC	5 cut(s)	372, 1258, 1417, 1835, 2113
BceAI	ACGGC	2 cut(s)	639, 1674
BciT130I	CCWGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
BclI	TGATCA	2 cut(s)	931, 1725
BcoDI	GTCTC	5 cut(s)	46, 822, 1328, 1403, 1607
BcuI	ACTAGT	1 cut(s)	1234
BfaI	CTAG	8 cut(s)	278, 614, 663, 777, 1235, 1302, 1736, 1982
BfmI	CTRYAG	2 cut(s)	201, 1803
BfoI	RGCGCY	1 cut(s)	116
BfuAI	ACCTGC	1 cut(s)	1848
BglII	AGATCT	1 cut(s)	1852
BisI	GCNGC	5 cut(s)	506, 747, 1748, 1803, 1924
BlpI	GCTNAGC	2 cut(s)	288, 1102
BlsI	GCNGC	5 cut(s)	507, 748, 1749, 1804, 1925
Bme1390I	CCNGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
Bme18I	GGWCC	1 cut(s)	334
BmgT120I	GGNCC	2 cut(s)	334, 2102
BmiI	GGNNCC	3 cut(s)	114, 1603, 1694
BmrFI	CCNGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
BmrI	ACTGGG	1 cut(s)	1736
BmsI	GCATC	7 cut(s)	227, 953, 1151, 1198, 1309, 1789, 1887
BmuI	ACTGGG	1 cut(s)	1736
BpmI	CTGGAG	1 cut(s)	1607
Bpu1102I	GCTNAGC	2 cut(s)	288, 1102
BpuEI	CTTGAG	2 cut(s)	29, 1631
Bsa29I	ATCGAT	1 cut(s)	1317
BsaBI	GATNNNNATC	1 cut(s)	1857
BsaHI	GRCGYC	1 cut(s)	113
BsaI	GGTCTC	1 cut(s)	822
BsaJI	CCNNGG	7 cut(s)	330, 812, 1924, 1994, 2098, 2099, 2113
BsaWI	WCCGGW	1 cut(s)	336
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 27, 102, 336, 1064, 1703, 1985, 2100
Bse118I	RCCGGY	1 cut(s)	985
Bse1I	ACTGG	4 cut(s)	85, 379, 761, 1742
Bse3DI	GCAATG	2 cut(s)	498, 604
Bse8I	GATNNNNATC	1 cut(s)	1857
BseBI	CCWGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
BseCI	ATCGAT	1 cut(s)	1317
BseDI	CCNNGG	7 cut(s)	330, 812, 1924, 1994, 2098, 2099, 2113
BseGI	GGATG	4 cut(s)	218, 383, 1804, 2004
BseJI	GATNNNNATC	1 cut(s)	1857
BseLI	CCNNNNNNNGG	8 cut(s)	26, 27, 102, 336, 1064, 1703, 1985, 2100
BseMI	GCAATG	2 cut(s)	498, 604
BseMII	CTCAG	4 cut(s)	279, 1414, 2027, 2051
BseNI	ACTGG	4 cut(s)	85, 379, 761, 1742
BseRI	GAGGAG	1 cut(s)	2055
BseSI	GKGCMC	2 cut(s)	2016, 2068
BseXI	GCAGC	2 cut(s)	517, 1789
BseYI	CCCAGC	1 cut(s)	1703
BsgI	GTGCAG	1 cut(s)	555
Bsh1236I	CGCG	4 cut(s)	27, 93, 457, 1926
BshFI	GGCC	6 cut(s)	261, 512, 630, 749, 1298, 2104
BshNI	GGYRCC	1 cut(s)	112
BshVI	ATCGAT	1 cut(s)	1317
BsiHKAI	GWGCWC	4 cut(s)	921, 1783, 2016, 2068
BsiSI	CCGG	3 cut(s)	96, 337, 986
BslFI	GGGAC	5 cut(s)	534, 758, 1275, 1724, 2085
BslI	CCNNNNNNNGG	8 cut(s)	26, 27, 102, 336, 1064, 1703, 1985, 2100
BsmAI	GTCTC	5 cut(s)	46, 822, 1328, 1403, 1607
BsmBI	CGTCTC	1 cut(s)	1403
BsmFI	GGGAC	5 cut(s)	534, 758, 1275, 1724, 2085
BsmI	GAATGC	2 cut(s)	1050, 1227
BsnI	GGCC	6 cut(s)	261, 512, 630, 749, 1298, 2104
Bso31I	GGTCTC	1 cut(s)	822
Bsp1286I	GDGCHC	4 cut(s)	921, 1783, 2016, 2068
Bsp1720I	GCTNAGC	2 cut(s)	288, 1102
Bsp19I	CCATGG	1 cut(s)	812
BspANI	GGCC	6 cut(s)	261, 512, 630, 749, 1298, 2104
BspCNI	CTCAG	4 cut(s)	280, 1413, 2028, 2052
BspDI	ATCGAT	1 cut(s)	1317
BspFNI	CGCG	4 cut(s)	27, 93, 457, 1926
BspLI	GGNNCC	3 cut(s)	114, 1603, 1694
BspMAI	CTGCAG	2 cut(s)	205, 1807
BspMI	ACCTGC	1 cut(s)	1848
BspPI	GGATC	6 cut(s)	113, 660, 1687, 1700, 1866, 1927
BspQI	GCTCTTC	1 cut(s)	413
BspT107I	GGYRCC	1 cut(s)	112
BspTNI	GGTCTC	1 cut(s)	822
BsrDI	GCAATG	2 cut(s)	498, 604
BsrFI	RCCGGY	1 cut(s)	985
BsrI	ACTGG	4 cut(s)	85, 379, 761, 1742
BssAI	RCCGGY	1 cut(s)	985
BssECI	CCNNGG	7 cut(s)	330, 812, 1924, 1994, 2098, 2099, 2113
BssNAI	GTATAC	2 cut(s)	309, 1163
BssNI	GRCGYC	1 cut(s)	113
BssSI	CACGAG	2 cut(s)	524, 2127
BssT1I	CCWWGG	3 cut(s)	330, 812, 1994
Bst1107I	GTATAC	2 cut(s)	309, 1163
Bst2BI	CACGAG	2 cut(s)	524, 2127
Bst2UI	CCWGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
Bst4CI	ACNGT	7 cut(s)	298, 798, 844, 938, 1339, 1618, 1889
Bst6I	CTCTTC	2 cut(s)	365, 413
BstACI	GRCGYC	1 cut(s)	113
BstC8I	GCNNGC	7 cut(s)	263, 417, 433, 510, 1140, 1309, 1517
BstDEI	CTNAG	7 cut(s)	288, 364, 1102, 1400, 1764, 2036, 2060
BstDSI	CCRYGG	2 cut(s)	812, 1924
BstF5I	GGATG	4 cut(s)	218, 383, 1804, 2004
BstFNI	CGCG	4 cut(s)	27, 93, 457, 1926
BstH2I	RGCGCY	1 cut(s)	116
BstHHI	GCGC	3 cut(s)	95, 115, 613
BstMAI	GTCTC	5 cut(s)	46, 822, 1328, 1403, 1607
BstMWI	GCNNNNNNNGC	6 cut(s)	412, 502, 1135, 1148, 1304, 1502
BstNI	CCWGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
BstNSI	RCATGY	1 cut(s)	435
BstSCI	CCNGG	6 cut(s)	368, 668, 820, 1519, 1794, 2098
BstSFI	CTRYAG	2 cut(s)	201, 1803
BstSLI	GKGCMC	2 cut(s)	2016, 2068
BstUI	CGCG	4 cut(s)	27, 93, 457, 1926
BstV1I	GCAGC	2 cut(s)	517, 1789
BstX2I	RGATCY	6 cut(s)	105, 665, 1692, 1852, 1858, 1932
BstXI	CCANNNNNNTGG	1 cut(s)	813
BstYI	RGATCY	6 cut(s)	105, 665, 1692, 1852, 1858, 1932
BstZ17I	GTATAC	2 cut(s)	309, 1163
Bsu15I	ATCGAT	1 cut(s)	1317
BsuRI	GGCC	6 cut(s)	261, 512, 630, 749, 1298, 2104
BsuTUI	ATCGAT	1 cut(s)	1317
BtgI	CCRYGG	2 cut(s)	812, 1924
BtsCI	GGATG	4 cut(s)	218, 383, 1804, 2004
BtsI	GCAGTG	3 cut(s)	572, 1557, 2014
BtsIMutI	CAGTG	6 cut(s)	572, 943, 1557, 1623, 1749, 2014
BveI	ACCTGC	1 cut(s)	1848
Cac8I	GCNNGC	7 cut(s)	263, 417, 433, 510, 1140, 1309, 1517
CaiI	CAGNNNCTG	1 cut(s)	268
CfoI	GCGC	3 cut(s)	95, 115, 613
Cfr10I	RCCGGY	1 cut(s)	985
Cfr13I	GGNCC	2 cut(s)	334, 2102
Cfr42I	CCGCGG	1 cut(s)	1927
ClaI	ATCGAT	1 cut(s)	1317
Csp6I	GTAC	2 cut(s)	206, 866
CviAII	CATG	8 cut(s)	432, 813, 1030, 1201, 1501, 1598, 1689, 1729
CviQI	GTAC	2 cut(s)	206, 866
DdeI	CTNAG	7 cut(s)	288, 364, 1102, 1400, 1764, 2036, 2060
DinI	GGCGCC	1 cut(s)	114
DraI	TTTAAA	1 cut(s)	2028
DraIII	CACNNNGTG	1 cut(s)	233
DrdI	GACNNNNNNGTC	1 cut(s)	801
DseDI	GACNNNNNNGTC	1 cut(s)	801
Eam1104I	CTCTTC	2 cut(s)	365, 413
EarI	CTCTTC	2 cut(s)	365, 413
EciI	GGCGGA	1 cut(s)	1925
Eco130I	CCWWGG	3 cut(s)	330, 812, 1994
Eco31I	GGTCTC	1 cut(s)	822
Eco47I	GGWCC	1 cut(s)	334
Eco57I	CTGAAG	2 cut(s)	123, 998
EcoRII	CCWGG	6 cut(s)	368, 668, 820, 1519, 1794, 2098
EcoT14I	CCWWGG	3 cut(s)	330, 812, 1994
EcoT22I	ATGCAT	2 cut(s)	1092, 1213
EgeI	GGCGCC	1 cut(s)	114
EheI	GGCGCC	1 cut(s)	114
ErhI	CCWWGG	3 cut(s)	330, 812, 1994
Esp3I	CGTCTC	1 cut(s)	1403
FaeI	CATG	8 cut(s)	435, 816, 1033, 1204, 1504, 1601, 1692, 1732
FaqI	GGGAC	5 cut(s)	534, 758, 1275, 1724, 2085
FatI	CATG	8 cut(s)	431, 812, 1029, 1200, 1500, 1597, 1688, 1728
FauI	CCCGC	1 cut(s)	20
FbaI	TGATCA	2 cut(s)	931, 1725
FblI	GTMKAC	3 cut(s)	308, 1162, 1239
Fnu4HI	GCNGC	5 cut(s)	506, 747, 1748, 1803, 1924
FokI	GGATG	4 cut(s)	205, 390, 1811, 2011
Fsp4HI	GCNGC	5 cut(s)	506, 747, 1748, 1803, 1924
FspBI	CTAG	8 cut(s)	278, 614, 663, 777, 1235, 1302, 1736, 1982
GlaI	GCGC	3 cut(s)	94, 114, 612
GluI	GCNGC	5 cut(s)	506, 747, 1748, 1803, 1924
GsaI	CCCAGC	1 cut(s)	1707
GsuI	CTGGAG	1 cut(s)	1607
HaeII	RGCGCY	1 cut(s)	116
HaeIII	GGCC	6 cut(s)	261, 512, 630, 749, 1298, 2104
HapII	CCGG	3 cut(s)	96, 337, 986
HhaI	GCGC	3 cut(s)	95, 115, 613
Hin1I	GRCGYC	1 cut(s)	113
Hin1II	CATG	8 cut(s)	435, 816, 1033, 1204, 1504, 1601, 1692, 1732
Hin6I	GCGC	3 cut(s)	93, 113, 611
HinP1I	GCGC	3 cut(s)	93, 113, 611
HindIII	AAGCTT	4 cut(s)	193, 1099, 1136, 1513
HinfI	GANTC	7 cut(s)	99, 211, 522, 783, 1228, 1280, 1594
HpaII	CCGG	3 cut(s)	96, 337, 986
HphI	GGTGA	3 cut(s)	479, 1775, 1940
Hpy166II	GTNNAC	8 cut(s)	89, 309, 393, 453, 1163, 1240, 2014, 2066
Hpy188I	TCNGA	3 cut(s)	567, 978, 2117
Hpy188III	TCNNGA	7 cut(s)	103, 1610, 1654, 1696, 1790, 1856, 1869
Hpy8I	GTNNAC	8 cut(s)	89, 309, 393, 453, 1163, 1240, 2014, 2066
HpyCH4III	ACNGT	7 cut(s)	298, 798, 844, 938, 1339, 1618, 1889
HpyCH4IV	ACGT	1 cut(s)	1396
HpyF10VI	GCNNNNNNNGC	6 cut(s)	412, 502, 1135, 1148, 1304, 1502
HpyF3I	CTNAG	7 cut(s)	288, 364, 1102, 1400, 1764, 2036, 2060
HpySE526I	ACGT	1 cut(s)	1396
Hsp92I	GRCGYC	1 cut(s)	113
Hsp92II	CATG	8 cut(s)	435, 816, 1033, 1204, 1504, 1601, 1692, 1732
HspAI	GCGC	3 cut(s)	93, 113, 611
KasI	GGCGCC	1 cut(s)	112
Ksp22I	TGATCA	2 cut(s)	931, 1725
KspI	CCGCGG	1 cut(s)	1927
LguI	GCTCTTC	1 cut(s)	413
LmnI	GCTCC	2 cut(s)	887, 1567
Lsp1109I	GCAGC	2 cut(s)	517, 1789
LweI	GCATC	7 cut(s)	227, 953, 1151, 1198, 1309, 1789, 1887
MaeI	CTAG	8 cut(s)	278, 614, 663, 777, 1235, 1302, 1736, 1982
MaeII	ACGT	1 cut(s)	1396
MaeIII	GTNAC	2 cut(s)	1636, 2001
MfeI	CAATTG	2 cut(s)	1292, 1632
MflI	RGATCY	6 cut(s)	105, 665, 1692, 1852, 1858, 1932
MhlI	GDGCHC	4 cut(s)	921, 1783, 2016, 2068
MluI	ACGCGT	1 cut(s)	455
Mly113I	GGCGCC	1 cut(s)	113
MlyI	GAGTC	3 cut(s)	205, 516, 1222
MmeI	TCCRAC	2 cut(s)	235, 865
Mph1103I	ATGCAT	2 cut(s)	1092, 1213
MroXI	GAANNNNTTC	1 cut(s)	359
MseI	TTAA	6 cut(s)	638, 701, 765, 972, 1685, 2027
MspA1I	CMGCKG	1 cut(s)	1926
MspI	CCGG	3 cut(s)	96, 337, 986
MspR9I	CCNGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
MunI	CAATTG	2 cut(s)	1292, 1632
Mva1269I	GAATGC	2 cut(s)	1050, 1227
MvaI	CCWGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
MvnI	CGCG	4 cut(s)	27, 93, 457, 1926
MwoI	GCNNNNNNNGC	6 cut(s)	412, 502, 1135, 1148, 1304, 1502
NarI	GGCGCC	1 cut(s)	113
NcoI	CCATGG	1 cut(s)	812
NlaIII	CATG	8 cut(s)	435, 816, 1033, 1204, 1504, 1601, 1692, 1732
NlaIV	GGNNCC	3 cut(s)	114, 1603, 1694
NmuCI	GTSAC	2 cut(s)	1636, 2001
NsiI	ATGCAT	2 cut(s)	1092, 1213
NspI	RCATGY	1 cut(s)	435
PaeI	GCATGC	1 cut(s)	435
PasI	CCCWGGG	1 cut(s)	2099
PciSI	GCTCTTC	1 cut(s)	413
PctI	GAATGC	2 cut(s)	1050, 1227
PdmI	GAANNNNTTC	1 cut(s)	359
PfeI	GAWTC	4 cut(s)	99, 783, 1280, 1594
PkrI	GCNGC	5 cut(s)	507, 748, 1749, 1804, 1925
PleI	GAGTC	3 cut(s)	205, 516, 1222
PluTI	GGCGCC	1 cut(s)	116
PpsI	GAGTC	3 cut(s)	205, 516, 1222
PshBI	ATTAAT	1 cut(s)	701
Psp6I	CCWGG	6 cut(s)	368, 668, 820, 1519, 1794, 2098
PspFI	CCCAGC	1 cut(s)	1703
PspGI	CCWGG	6 cut(s)	368, 668, 820, 1519, 1794, 2098
PspN4I	GGNNCC	3 cut(s)	114, 1603, 1694
PspPI	GGNCC	2 cut(s)	334, 2102
PstI	CTGCAG	2 cut(s)	205, 1807
PstNI	CAGNNNCTG	1 cut(s)	268
PsuI	RGATCY	6 cut(s)	105, 665, 1692, 1852, 1858, 1932
RsaI	GTAC	2 cut(s)	207, 867
RsaNI	GTAC	2 cut(s)	206, 866
SacII	CCGCGG	1 cut(s)	1927
SapI	GCTCTTC	1 cut(s)	413
SaqAI	TTAA	6 cut(s)	638, 701, 765, 972, 1685, 2027
SatI	GCNGC	5 cut(s)	506, 747, 1748, 1803, 1924
Sau96I	GGNCC	2 cut(s)	334, 2102
SchI	GAGTC	3 cut(s)	205, 516, 1222
ScrFI	CCNGG	6 cut(s)	370, 670, 822, 1521, 1796, 2100
SduI	GDGCHC	4 cut(s)	921, 1783, 2016, 2068
SfaNI	GCATC	7 cut(s)	227, 953, 1151, 1198, 1309, 1789, 1887
SfcI	CTRYAG	2 cut(s)	201, 1803
SfoI	GGCGCC	1 cut(s)	114
Sfr303I	CCGCGG	1 cut(s)	1927
SgrBI	CCGCGG	1 cut(s)	1927
SinI	GGWCC	1 cut(s)	334
SmlI	CTYRAG	2 cut(s)	44, 1610
SmoI	CTYRAG	2 cut(s)	44, 1610
SpeI	ACTAGT	1 cut(s)	1234
SphI	GCATGC	1 cut(s)	435
SspDI	GGCGCC	1 cut(s)	112
SspI	AATATT	1 cut(s)	1545
SspMI	CTAG	8 cut(s)	278, 614, 663, 777, 1235, 1302, 1736, 1982
StyD4I	CCNGG	6 cut(s)	368, 668, 820, 1519, 1794, 2098
StyI	CCWWGG	3 cut(s)	330, 812, 1994
TaaI	ACNGT	7 cut(s)	298, 798, 844, 938, 1339, 1618, 1889
TaiI	ACGT	1 cut(s)	1399
TaqI	TCGA	5 cut(s)	174, 248, 1153, 1317, 1570
TatI	WGTACW	1 cut(s)	205
TauI	GCSGC	3 cut(s)	749, 1750, 1926
TfiI	GAWTC	4 cut(s)	99, 783, 1280, 1594
Tru1I	TTAA	6 cut(s)	638, 701, 765, 972, 1685, 2027
Tru9I	TTAA	6 cut(s)	638, 701, 765, 972, 1685, 2027
TscAI	CASTG	6 cut(s)	579, 943, 1564, 1623, 1749, 2021
TseFI	GTSAC	2 cut(s)	1636, 2001
TseI	GCWGC	2 cut(s)	505, 1802
Tsp45I	GTSAC	2 cut(s)	1636, 2001
TspDTI	ATGAA	6 cut(s)	875, 981, 1217, 1489, 1920, 2046
TspGWI	ACGGA	1 cut(s)	1233
TspRI	CASTG	6 cut(s)	579, 943, 1564, 1623, 1749, 2021
VneI	GTGCAC	2 cut(s)	2012, 2064
VpaK11BI	GGWCC	1 cut(s)	334
VspI	ATTAAT	1 cut(s)	701
XapI	RAATTY	3 cut(s)	533, 968, 1171
XceI	RCATGY	1 cut(s)	435
XcmI	CCANNNNNNNNNTGG	2 cut(s)	251, 1291
XmiI	GTMKAC	3 cut(s)	308, 1162, 1239
XmnI	GAANNNNTTC	1 cut(s)	359
XspI	CTAG	8 cut(s)	278, 614, 663, 777, 1235, 1302, 1736, 1982
Zsp2I	ATGCAT	2 cut(s)	1092, 1213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.