Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001955

Physical Location & Seq

Genomic Coordinates Reverse (-)

34025362 .. 34030887

Gene Structure 5526 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1279900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGAAGAGACATTTGTGCCATTGCAGGGAATCAAGAATGATCTCAGAGGAAGATTGAAGTGCTACAAGGAAGACTGGACTGGGGGCTTTAAAGCTGGATTCAGGATTTTGGCTCCTACCACCTACATATTTTTTGCTTCTGCAATCCCAGTCATTTCATTTGGTGAACAACTGGAGCGAAGTACTGGTGGAGTTTTGACAGCAGTGCAAACCTTAGTATCGACGGCAGTTTGTGGGATCATACACTCCATCGTCGGAGGTCAACCCTTGCTGATATTGGGTGTGGCCGAGCCTACTGTAATCATGTACACCTTCATGTTCAATTTTGCCAAAGAACGTACAGACTTGGGTCCAAAGCTCTTTCTAGCATGGTCCGCATGGGTATGTGTATGGACAGCTGGGTTACTGTTCTTATTTGCTATATTAGGTGCTTGTTCCATAATAAATAGGTTTACAAGAGTGGCTGGGGAGTTGTTTGGTCTGCTAATCGCCATGCTTTTCATGCAGCAAGCCATTAAAGGAATTGTGGATGAGTTTCGCTTACCAGAAGGAAAAGATACTAGCCTACTACAATTTATACCTTCATGGAGGTTTGCAAATGGAATGTTTGCTTTGGTGTTGTCATTTGGCCTTCTTCTCACTGCATTAAAAAGCAGGAAAGCAAGGTCATGGCGCTATGGCACTGGTTGGCTAAGAAGCTTTGTAGCAGACTATGGTGTGCCACTTATGGTTCTGGTGTGGACTGGTGTGTCTTACATTCCAACTAGTAGTGTTCCACATGGCATCCCAAGGCGCCTTTTAAGCCCAAATCCATGGTCGCCTGGCGCATATGACAATTGGACTGTCATTAAGGACATGCTCAATGTTCCGGTCCTCTATATCATCGGAGCTTTCATTCCAGCAACGATGATCTCAGTGCTTTACTATTTTGATCACAGTGTAGCATCCCAACTATCTCAGCAGAAAGAGTTCAATTTAAGAAAGCCATCTTCTTACCATTATGACTTGCTTCTTTTGGGGTTTCTGACTTTAATGTGTGGTCTGCTTGGAATTCCTCCGTCAAATGGAGTTATCCCACAGTCTCCAATGCATACAAAGAGTTTAGCTACTCTTAAACACCAGTTGCTTCGTAATCGACTTGTAGCAACAGCACGCACAAGTATGAGAAATAATTCTAGCTTGGGACAACTGTATGGAAATATGCAAGATGCCTATCGACAAATGCAGACGCCTTTGGTTTACCAGGAGGCCTCATCTCGAGGACTGAATGAATTAAAAGAATCAACCATCCAGGGAGCTTCAAGTATGGGAAATTATATTGATGCACCAGTTGATGAGACAGTATTTGATATTGAGAAAGAGATTGATGATTTATTGCCGGTTGAGGTGAAGGAGCAGCGTCTCAGCAACCTGCTTCAAGCGGTATTGGTGGGGGGATGTGTTGCAGCCATGCCTATCCTCAAAAGGATCCCTACTTCAGTCCTTTGGGGATACTTTGCCTTCATGGCCATTGAAAGCTTACCCGGTAACCAATTTTGGGAACGGATCTGTTTGCTCTTTACAGCTCCAAGTAGAAGATTCAAAGTCCTCGAGGATAATCACGCCACTTTTGTAGAAACTGTGCCTTTCAAGTCAATCGCGATGTTCACAGTTTTCCAAACTCTGTACTTGCTTGTATGTTTTTTGCTTACTTGGGTTCCAATTGCTGGGGTTATGTTTCCAATGATGATCATGCTCTTGGTTCCTGTGAGGCAATACATACTCCCCAAGTTTTTCAAAGGAGCACACCTTCAGGACTTGGATGCAGCAGAGTACGAAGAGGCTCCGGCTTTAACATATAACCTAGCAACGGAGACAGAGCTGGGAGCTGGAGCTTCCTGCGCAGGGGATGCAGAAATATTAGACGAAGTTATGACCAGAAGCCGGGGTGAGTTTAGGCATGTCAGCAGTCCAAAGATCACAAGCTCTACTTCAACACCGGCAAATGCCTCTGAAATCCTTGCGAGTCCACACCCGACCTTCAGTCCTCGCGTAAGTGGGCTAAGAGGAGAGCGAAGTCCTCATTCTGGCGGAAGAGGGTCACACAGTCCAAGGACCCCTGGCTCTTCTTCTATGCTTGGGAAGAGTCCTAGTAACAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006873 GO:0006885 GO:0007275 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0022622 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0071944 GO:0098771 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

714

Amino Acids

78.79

Weight (kDa)

8.78

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	8 - 179	3.4e-33	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	201 - 372	3.2e-24	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	461 - 603	4.1e-31	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47160 AT2G47160 AT3G62270
fragaria_vesca	FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400
malus_domestica	MD06G1173100.v1.1 MD09G1287700.v1.1 MD14G1179400.v1.1 MD17G1279900.v1.1
prunus_persica	Prupe.3G005600_v2.0.a1 Prupe.5G172600_v2.0.a1
pyrus_communis	pycom06g15420 pycom14g14930 pycom14g14940
rosa_chinensis	RchiOBHm_Chr3g0486761 RchiOBHm_Chr7g0193431
rosa_laevigata	RLG00000004268 RLG00000023062
rosa_multiflora	Rmu_sc0006629.1_g000001 Rmu_sc0012783.1_g000009 Rmu_sc0013001.1_g000011
rosa_roxburghii	Rroxscaffold_3G00262090 Rroxscaffold_6G00395610
rosa_rugosa	Rorug03G0227000 Rorug03G0227100 Rorug07G0009500
rosa_samantha	Rh3AG276900 Rh3BG311800 Rh3CG311000 Rh3DG307500 Rh7AG136100 Rh7BG136500 Rh7CG140000 Rh7DG137800
rosa_wichuraiana	Rw3G024620 Rw7G011610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	748
AbsI	CCTCGAGG	1 cut(s)	1589
Acc16I	TGCGCA	1 cut(s)	1882
Acc36I	ACCTGC	1 cut(s)	1419
AccB1I	GGYRCC	1 cut(s)	792
AccB7I	CCANNNNNTGG	1 cut(s)	1706
AccII	CGCG	2 cut(s)	1640, 2031
AciI	CCGC	3 cut(s)	375, 1421, 2070
AclWI	GGATC	4 cut(s)	245, 1462, 1475, 1553
AcoI	YGGCCR	2 cut(s)	285, 1506
AcsI	RAATTY	1 cut(s)	1050
AcuI	CTGAAG	3 cut(s)	1461, 1775, 2005
AcyI	GRCGYC	2 cut(s)	793, 1229
AfaI	GTAC	5 cut(s)	184, 308, 340, 1667, 1814
AfiI	CCNNNNNNNGG	8 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 1923, 2066
AhdI	GACNNNNNGTC	1 cut(s)	2022
AhlI	ACTAGT	1 cut(s)	764
AjnI	CCWGG	4 cut(s)	820, 1242, 1290, 2098
Alw21I	GWGCWC	1 cut(s)	1786
Alw26I	GTCTC	5 cut(s)	2, 1086, 1331, 1406, 1847
AlwI	GGATC	4 cut(s)	245, 1462, 1475, 1553
Ama87I	CYCGRG	2 cut(s)	1257, 1589
AoxI	GGCC	4 cut(s)	285, 628, 1248, 1506
ApeKI	GCWGC	4 cut(s)	505, 1396, 1445, 1805
ApoI	RAATTY	1 cut(s)	1050
ArsI	GACNNNNNNTTYG	2 cut(s)	800, 832
Asp700I	GAANNNNTTC	2 cut(s)	968, 1171
AspLEI	GCGC	4 cut(s)	675, 795, 827, 1883
AspS9I	GGNCC	4 cut(s)	350, 372, 871, 2094
AsuC2I	CCSGG	2 cut(s)	1524, 1925
AsuHPI	GGTGA	3 cut(s)	176, 1399, 1940
AvaI	CYCGRG	2 cut(s)	1257, 1589
AvaII	GGWCC	4 cut(s)	350, 372, 871, 2094
BaeI	ACNNNNGTAYC	2 cut(s)	201, 234
BalI	TGGCCA	1 cut(s)	1508
BamHI	GGATCC	1 cut(s)	1467
BanI	GGYRCC	1 cut(s)	792
BbsI	GAAGAC	1 cut(s)	78
Bbv12I	GWGCWC	1 cut(s)	1786
BbvI	GCAGC	4 cut(s)	517, 1408, 1457, 1817
BccI	CCATC	3 cut(s)	257, 994, 1295
BceAI	ACGGC	1 cut(s)	240
BciT130I	CCWGG	4 cut(s)	822, 1244, 1292, 2100
BciVI	GTATCC	1 cut(s)	1484
BclI	TGATCA	2 cut(s)	931, 1728
BcnI	CCSGG	2 cut(s)	1524, 1925
BcoDI	GTCTC	5 cut(s)	2, 1086, 1331, 1406, 1847
BcuI	ACTAGT	1 cut(s)	764
BfaI	CTAG	6 cut(s)	365, 561, 765, 1176, 1844, 2130
BfoI	RGCGCY	2 cut(s)	676, 796
BfuAI	ACCTGC	1 cut(s)	1419
BfuI	GTATCC	1 cut(s)	1484
BglI	GCCNNNNNGGC	1 cut(s)	1505
BisI	GCNGC	4 cut(s)	506, 1397, 1446, 1806
BlsI	GCNGC	4 cut(s)	507, 1398, 1447, 1807
BmcAI	AGTACT	1 cut(s)	184
Bme1390I	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 1925, 2100
Bme18I	GGWCC	4 cut(s)	350, 372, 871, 2094
BmeRI	GACNNNNNGTC	1 cut(s)	2022
BmeT110I	CYCGRG	2 cut(s)	1257, 1589
BmgT120I	GGNCC	4 cut(s)	350, 372, 871, 2094
BmiI	GGNNCC	8 cut(s)	114, 351, 794, 1469, 1698, 1743, 1824, 2096
BmrFI	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 1925, 2100
BmrI	ACTGGG	2 cut(s)	90, 143
BmsI	GCATC	6 cut(s)	792, 953, 1198, 1312, 1792, 1879
BmuI	ACTGGG	2 cut(s)	90, 143
BoxI	GACNNNNGTC	1 cut(s)	348
BpiI	GAAGAC	1 cut(s)	78
BpmI	CTGGAG	2 cut(s)	194, 1890
BpuMI	CCSGG	2 cut(s)	1524, 1925
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaHI	GRCGYC	2 cut(s)	793, 1229
BsaJI	CCNNGG	6 cut(s)	788, 812, 1291, 1924, 2090, 2098
BsaWI	WCCGGW	1 cut(s)	868
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 1923, 2066
Bse118I	RCCGGY	2 cut(s)	1378, 1978
Bse1I	ACTGG	9 cut(s)	80, 85, 149, 177, 190, 688, 748, 1120, 1328
Bse3DI	GCAATG	1 cut(s)	20
Bse8I	GATNNNNATC	1 cut(s)	1212
BseBI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
BseDI	CCNNGG	6 cut(s)	788, 812, 1291, 1924, 2090, 2098
BseGI	GGATG	7 cut(s)	535, 783, 944, 1287, 1442, 1807, 1894
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	8 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 1923, 2066
BseMI	GCAATG	1 cut(s)	20
BseMII	CTCAG	4 cut(s)	58, 927, 971, 1417
BseNI	ACTGG	9 cut(s)	80, 85, 149, 177, 190, 688, 748, 1120, 1328
BseRI	GAGGAG	1 cut(s)	2061
BseXI	GCAGC	4 cut(s)	517, 1408, 1457, 1817
BseYI	CCCAGC	4 cut(s)	398, 464, 1706, 1861
Bsh1236I	CGCG	2 cut(s)	1640, 2031
BshFI	GGCC	4 cut(s)	287, 630, 1250, 1508
BshNI	GGYRCC	1 cut(s)	792
BsiHKAI	GWGCWC	1 cut(s)	1786
BsiHKCI	CYCGRG	2 cut(s)	1257, 1589
BsiSI	CCGG	6 cut(s)	869, 1379, 1524, 1826, 1924, 1979
BslFI	GGGAC	1 cut(s)	1197
BslI	CCNNNNNNNGG	8 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 1923, 2066
BsmAI	GTCTC	5 cut(s)	2, 1086, 1331, 1406, 1847
BsmBI	CGTCTC	1 cut(s)	1406
BsmFI	GGGAC	1 cut(s)	1197
BsnI	GGCC	4 cut(s)	287, 630, 1250, 1508
BsoBI	CYCGRG	2 cut(s)	1257, 1589
Bsp1286I	GDGCHC	1 cut(s)	1786
Bsp1407I	TGTACA	1 cut(s)	306
Bsp143I	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
Bsp19I	CCATGG	1 cut(s)	812
Bsp68I	TCGCGA	1 cut(s)	1640
BspACI	CCGC	3 cut(s)	375, 1421, 2070
BspANI	GGCC	4 cut(s)	287, 630, 1250, 1508
BspCNI	CTCAG	4 cut(s)	57, 926, 970, 1416
BspFNI	CGCG	2 cut(s)	1640, 2031
BspLI	GGNNCC	8 cut(s)	114, 351, 794, 1469, 1698, 1743, 1824, 2096
BspMI	ACCTGC	1 cut(s)	1419
BspPI	GGATC	4 cut(s)	245, 1462, 1475, 1553
BspQI	GCTCTTC	1 cut(s)	2110
BspT107I	GGYRCC	1 cut(s)	792
BsrDI	GCAATG	1 cut(s)	20
BsrFI	RCCGGY	2 cut(s)	1378, 1978
BsrGI	TGTACA	1 cut(s)	306
BsrI	ACTGG	9 cut(s)	80, 85, 149, 177, 190, 688, 748, 1120, 1328
BssAI	RCCGGY	2 cut(s)	1378, 1978
BssECI	CCNNGG	6 cut(s)	788, 812, 1291, 1924, 2090, 2098
BssMI	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
BssNI	GRCGYC	2 cut(s)	793, 1229
BssT1I	CCWWGG	3 cut(s)	788, 812, 2090
Bst2UI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
Bst6I	CTCTTC	4 cut(s)	1812, 2068, 2110, 2117
BstACI	GRCGYC	2 cut(s)	793, 1229
BstAPI	GCANNNNNTGC	1 cut(s)	1889
BstAUI	TGTACA	1 cut(s)	306
BstC8I	GCNNGC	2 cut(s)	510, 1153
BstDEI	CTNAG	7 cut(s)	44, 214, 692, 913, 957, 1403, 2042
BstDSI	CCRYGG	1 cut(s)	812
BstEII	GGTNACC	1 cut(s)	1526
BstF5I	GGATG	7 cut(s)	535, 783, 944, 1287, 1442, 1807, 1894
BstFNI	CGCG	2 cut(s)	1640, 2031
BstH2I	RGCGCY	2 cut(s)	676, 796
BstHHI	GCGC	4 cut(s)	675, 795, 827, 1883
BstKTI	GATC	8 cut(s)	43, 240, 912, 934, 1470, 1548, 1731, 1959
BstMAI	GTCTC	5 cut(s)	2, 1086, 1331, 1406, 1847
BstMBI	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
BstMWI	GCNNNNNNNGC	6 cut(s)	374, 502, 801, 1505, 1880, 1889
BstNI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
BstNSI	RCATGY	2 cut(s)	859, 1943
BstPAI	GACNNNNGTC	1 cut(s)	348
BstPI	GGTNACC	1 cut(s)	1526
BstSCI	CCNGG	6 cut(s)	820, 1242, 1290, 1522, 1923, 2098
BstUI	CGCG	2 cut(s)	1640, 2031
BstV1I	GCAGC	4 cut(s)	517, 1408, 1457, 1817
BstV2I	GAAGAC	1 cut(s)	78
BstX2I	RGATCY	2 cut(s)	1467, 1545
BstXI	CCANNNNNNTGG	1 cut(s)	813
BstYI	RGATCY	2 cut(s)	1467, 1545
BsuI	GTATCC	1 cut(s)	1484
BsuRI	GGCC	4 cut(s)	287, 630, 1250, 1508
BtgI	CCRYGG	1 cut(s)	812
BtgZI	GCGATG	1 cut(s)	1655
BtsCI	GGATG	7 cut(s)	535, 783, 944, 1287, 1442, 1807, 1894
BtsI	GCAGTG	2 cut(s)	210, 639
BtsIMutI	CAGTG	5 cut(s)	210, 639, 681, 921, 943
BtuMI	TCGCGA	1 cut(s)	1640
BveI	ACCTGC	1 cut(s)	1419
Cac8I	GCNNGC	2 cut(s)	510, 1153
CfoI	GCGC	4 cut(s)	675, 795, 827, 1883
Cfr10I	RCCGGY	2 cut(s)	1378, 1978
Cfr13I	GGNCC	4 cut(s)	350, 372, 871, 2094
CseI	GACGC	2 cut(s)	1237, 1388
Csp6I	GTAC	5 cut(s)	183, 307, 339, 1666, 1813
CviQI	GTAC	5 cut(s)	183, 307, 339, 1666, 1813
DdeI	CTNAG	7 cut(s)	44, 214, 692, 913, 957, 1403, 2042
DinI	GGCGCC	1 cut(s)	794
DpnI	GATC	8 cut(s)	42, 239, 911, 933, 1469, 1547, 1730, 1958
DpnII	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
DraI	TTTAAA	1 cut(s)	91
DrdI	GACNNNNNNGTC	1 cut(s)	748
DriI	GACNNNNNGTC	1 cut(s)	2022
DseDI	GACNNNNNNGTC	1 cut(s)	748
EaeI	YGGCCR	2 cut(s)	285, 1506
Eam1104I	CTCTTC	4 cut(s)	1812, 2068, 2110, 2117
Eam1105I	GACNNNNNGTC	1 cut(s)	2022
EarI	CTCTTC	4 cut(s)	1812, 2068, 2110, 2117
EciI	GGCGGA	1 cut(s)	2085
Eco130I	CCWWGG	3 cut(s)	788, 812, 2090
Eco147I	AGGCCT	1 cut(s)	1250
Eco47I	GGWCC	4 cut(s)	350, 372, 871, 2094
Eco57I	CTGAAG	3 cut(s)	1461, 1775, 2005
Eco88I	CYCGRG	2 cut(s)	1257, 1589
Eco91I	GGTNACC	1 cut(s)	1526
EcoO109I	RGGNCCY	1 cut(s)	2094
EcoO65I	GGTNACC	1 cut(s)	1526
EcoRI	GAATTC	1 cut(s)	1050
EcoRII	CCWGG	4 cut(s)	820, 1242, 1290, 2098
EcoT14I	CCWWGG	3 cut(s)	788, 812, 2090
EcoT22I	ATGCAT	1 cut(s)	1092
EgeI	GGCGCC	1 cut(s)	794
EheI	GGCGCC	1 cut(s)	794
ErhI	CCWWGG	3 cut(s)	788, 812, 2090
Esp3I	CGTCTC	1 cut(s)	1406
FalI	AAGNNNNNCTT	2 cut(s)	1954, 1986
FaqI	GGGAC	1 cut(s)	1197
FauNDI	CATATG	1 cut(s)	829
FbaI	TGATCA	2 cut(s)	931, 1728
Fnu4HI	GCNGC	4 cut(s)	506, 1397, 1446, 1806
FokI	GGATG	7 cut(s)	542, 770, 931, 1274, 1449, 1814, 1901
Fsp4HI	GCNGC	4 cut(s)	506, 1397, 1446, 1806
FspBI	CTAG	6 cut(s)	365, 561, 765, 1176, 1844, 2130
FspI	TGCGCA	1 cut(s)	1882
GlaI	GCGC	4 cut(s)	674, 794, 826, 1882
GluI	GCNGC	4 cut(s)	506, 1397, 1446, 1806
GsaI	CCCAGC	4 cut(s)	402, 468, 1710, 1865
GsuI	CTGGAG	2 cut(s)	194, 1890
HaeII	RGCGCY	2 cut(s)	676, 796
HaeIII	GGCC	4 cut(s)	287, 630, 1250, 1508
HapII	CCGG	6 cut(s)	869, 1379, 1524, 1826, 1924, 1979
HgaI	GACGC	2 cut(s)	1237, 1388
HhaI	GCGC	4 cut(s)	675, 795, 827, 1883
Hin1I	GRCGYC	2 cut(s)	793, 1229
Hin6I	GCGC	4 cut(s)	673, 793, 825, 1881
HinP1I	GCGC	4 cut(s)	673, 793, 825, 1881
HincII	GTYRAC	1 cut(s)	263
HindII	GTYRAC	1 cut(s)	263
HindIII	AAGCTT	2 cut(s)	697, 1516
HinfI	GANTC	6 cut(s)	30, 99, 1280, 1578, 2005, 2125
HpaII	CCGG	6 cut(s)	869, 1379, 1524, 1826, 1924, 1979
HphI	GGTGA	3 cut(s)	176, 1399, 1940
Hpy166II	GTNNAC	8 cut(s)	167, 263, 309, 453, 741, 1240, 1647, 2009
Hpy188I	TCNGA	5 cut(s)	47, 257, 887, 1026, 1993
Hpy188III	TCNNGA	5 cut(s)	34, 103, 1257, 1639, 1793
Hpy8I	GTNNAC	8 cut(s)	167, 263, 309, 453, 741, 1240, 1647, 2009
Hpy99I	CGWCG	2 cut(s)	226, 257
HpyAV	CCTTC	8 cut(s)	322, 542, 591, 641, 1384, 1510, 1799, 2029
HpyCH4IV	ACGT	1 cut(s)	337
HpyF10VI	GCNNNNNNNGC	6 cut(s)	374, 502, 801, 1505, 1880, 1889
HpyF3I	CTNAG	7 cut(s)	44, 214, 692, 913, 957, 1403, 2042
HpySE526I	ACGT	1 cut(s)	337
Hsp92I	GRCGYC	2 cut(s)	793, 1229
HspAI	GCGC	4 cut(s)	673, 793, 825, 1881
KasI	GGCGCC	1 cut(s)	792
Ksp22I	TGATCA	2 cut(s)	931, 1728
Kzo9I	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
LguI	GCTCTTC	1 cut(s)	2110
Lsp1109I	GCAGC	4 cut(s)	517, 1408, 1457, 1817
LweI	GCATC	6 cut(s)	792, 953, 1198, 1312, 1792, 1879
MaeI	CTAG	6 cut(s)	365, 561, 765, 1176, 1844, 2130
MaeII	ACGT	1 cut(s)	337
MaeIII	GTNAC	4 cut(s)	402, 1526, 2079, 2132
MalI	GATC	8 cut(s)	42, 239, 911, 933, 1469, 1547, 1730, 1958
MboI	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
MfeI	CAATTG	2 cut(s)	835, 1701
MflI	RGATCY	2 cut(s)	1467, 1545
MhlI	GDGCHC	1 cut(s)	1786
MlsI	TGGCCA	1 cut(s)	1508
MluNI	TGGCCA	1 cut(s)	1508
Mly113I	GGCGCC	1 cut(s)	793
MlyI	GAGTC	2 cut(s)	2014, 2134
MmeI	TCCRAC	2 cut(s)	235, 785
Mox20I	TGGCCA	1 cut(s)	1508
Mph1103I	ATGCAT	1 cut(s)	1092
MroXI	GAANNNNTTC	2 cut(s)	968, 1171
MscI	TGGCCA	1 cut(s)	1508
MslI	CAYNNNNRTG	3 cut(s)	314, 382, 1160
Msp20I	TGGCCA	1 cut(s)	1508
MspA1I	CMGCKG	1 cut(s)	398
MspI	CCGG	6 cut(s)	869, 1379, 1524, 1826, 1924, 1979
MspR9I	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 1925, 2100
MunI	CAATTG	2 cut(s)	835, 1701
MvaI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
MvnI	CGCG	2 cut(s)	1640, 2031
MwoI	GCNNNNNNNGC	6 cut(s)	374, 502, 801, 1505, 1880, 1889
NarI	GGCGCC	1 cut(s)	793
NciI	CCSGG	2 cut(s)	1524, 1925
NcoI	CCATGG	1 cut(s)	812
NdeI	CATATG	1 cut(s)	829
NdeII	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
NlaIV	GGNNCC	8 cut(s)	114, 351, 794, 1469, 1698, 1743, 1824, 2096
NmeAIII	GCCGAG	1 cut(s)	313
NmuCI	GTSAC	1 cut(s)	2079
NruI	TCGCGA	1 cut(s)	1640
NsbI	TGCGCA	1 cut(s)	1882
NsiI	ATGCAT	1 cut(s)	1092
NspI	RCATGY	2 cut(s)	859, 1943
PaeR7I	CTCGAG	2 cut(s)	1257, 1589
PceI	AGGCCT	1 cut(s)	1250
PciSI	GCTCTTC	1 cut(s)	2110
PdmI	GAANNNNTTC	2 cut(s)	968, 1171
PfeI	GAWTC	4 cut(s)	30, 99, 1280, 1578
PflMI	CCANNNNNTGG	1 cut(s)	1706
PkrI	GCNGC	4 cut(s)	507, 1398, 1447, 1807
PleI	GAGTC	2 cut(s)	2013, 2133
PluTI	GGCGCC	1 cut(s)	796
PpsI	GAGTC	2 cut(s)	2013, 2133
PpuMI	RGGWCCY	1 cut(s)	2094
PshAI	GACNNNNGTC	1 cut(s)	348
Psp5II	RGGWCCY	1 cut(s)	2094
Psp6I	CCWGG	4 cut(s)	820, 1242, 1290, 2098
PspEI	GGTNACC	1 cut(s)	1526
PspFI	CCCAGC	4 cut(s)	398, 464, 1706, 1861
PspGI	CCWGG	4 cut(s)	820, 1242, 1290, 2098
PspN4I	GGNNCC	8 cut(s)	114, 351, 794, 1469, 1698, 1743, 1824, 2096
PspPI	GGNCC	4 cut(s)	350, 372, 871, 2094
PspPPI	RGGWCCY	1 cut(s)	2094
PspXI	VCTCGAGB	1 cut(s)	1589
PsuI	RGATCY	2 cut(s)	1467, 1545
PvuII	CAGCTG	1 cut(s)	398
RruI	TCGCGA	1 cut(s)	1640
RsaI	GTAC	5 cut(s)	184, 308, 340, 1667, 1814
RsaNI	GTAC	5 cut(s)	183, 307, 339, 1666, 1813
RseI	CAYNNNNRTG	3 cut(s)	314, 382, 1160
SapI	GCTCTTC	1 cut(s)	2110
SatI	GCNGC	4 cut(s)	506, 1397, 1446, 1806
Sau3AI	GATC	8 cut(s)	40, 237, 909, 931, 1467, 1545, 1728, 1956
Sau96I	GGNCC	4 cut(s)	350, 372, 871, 2094
ScaI	AGTACT	1 cut(s)	184
SchI	GAGTC	2 cut(s)	2014, 2134
ScrFI	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 1925, 2100
SduI	GDGCHC	1 cut(s)	1786
SfaNI	GCATC	6 cut(s)	792, 953, 1198, 1312, 1792, 1879
SfoI	GGCGCC	1 cut(s)	794
Sfr274I	CTCGAG	2 cut(s)	1257, 1589
SinI	GGWCC	4 cut(s)	350, 372, 871, 2094
SlaI	CTCGAG	2 cut(s)	1257, 1589
SmiMI	CAYNNNNRTG	3 cut(s)	314, 382, 1160
SmlI	CTYRAG	2 cut(s)	1257, 1589
SmoI	CTYRAG	2 cut(s)	1257, 1589
SpeI	ACTAGT	1 cut(s)	764
SseBI	AGGCCT	1 cut(s)	1250
SsiI	CCGC	3 cut(s)	375, 1421, 2070
SspDI	GGCGCC	1 cut(s)	792
SspI	AATATT	1 cut(s)	1899
SspMI	CTAG	6 cut(s)	365, 561, 765, 1176, 1844, 2130
StuI	AGGCCT	1 cut(s)	1250
StyD4I	CCNGG	6 cut(s)	820, 1242, 1290, 1522, 1923, 2098
StyI	CCWWGG	3 cut(s)	788, 812, 2090
TaiI	ACGT	1 cut(s)	340
TaqI	TCGA	5 cut(s)	221, 1135, 1216, 1258, 1590
TatI	WGTACW	3 cut(s)	182, 306, 1665
TfiI	GAWTC	4 cut(s)	30, 99, 1280, 1578
TscAI	CASTG	5 cut(s)	210, 646, 688, 921, 943
TseFI	GTSAC	1 cut(s)	2079
TseI	GCWGC	4 cut(s)	505, 1396, 1445, 1805
Tsp45I	GTSAC	1 cut(s)	2079
TspDTI	ATGAA	7 cut(s)	147, 304, 490, 573, 883, 1284, 1492
TspGWI	ACGGA	3 cut(s)	1047, 1558, 1865
TspRI	CASTG	5 cut(s)	210, 646, 688, 921, 943
Van91I	CCANNNNNTGG	1 cut(s)	1706
VpaK11BI	GGWCC	4 cut(s)	350, 372, 871, 2094
XapI	RAATTY	1 cut(s)	1050
XceI	RCATGY	2 cut(s)	859, 1943
XhoI	CTCGAG	2 cut(s)	1257, 1589
XmnI	GAANNNNTTC	2 cut(s)	968, 1171
XspI	CTAG	6 cut(s)	365, 561, 765, 1176, 1844, 2130
ZrmI	AGTACT	1 cut(s)	184
Zsp2I	ATGCAT	1 cut(s)	1092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.