Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001955

Physical Location & Seq

Genomic Coordinates Reverse (-)

10874845 .. 10878911

Gene Structure 4067 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G011610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGAAGAAACGTTTGTTCCTTTTCGCGGGATCAAAAATGACCTCAAAGGAAGAATTCTTTGCTACAAACAAGATTGGGCTGGTGGTTTACGAGCGGGTATAAGGATCTTGGCCCCAACAACATATGTATTCTTTGCTTCAACAATTCCAGTTATATCTTTTGGGGAGCAGCTGGAAAGAAATACAAATGGAACTTTGACTGCAGTACAGACTCTTGCATCAACTGCAATTTGTGGTGTTATCCATTCAATTGTTGGAGGGCAGCCCCTGCTCATACTAGGGGTGGCTGAACCAACAGTATTGATGTATACATTTATGTTCAACTTTGCTAAGGACCGAAAGGATTTGGGACAGGAACTCTTTTTAGCTTGGACTGGATGGGTATGTGTGTGGACATCCATTTTGCTCTTCTTGCTGGCCATTCTGGGTGCTTGCTCTATAATCAATAGGTTTACGCGTATTGCTGGTGAATTATTTGGTCTTCTTATCGCAATGCTTTTTATGCAGCAGGCCATAAGAGGAGTTGTAGAGGAATTTGGCGTACCTGAAAGAGAAAAGCCAAATCAGACTGCACTCCTACCTTCTTGGAGGTTTGGCAATGGAATGTTTGCATTAGTTCTGTCATTTGGCCTTCTATTTACTGCATTGAGAAGTCGTAAAGCTAGATCGTGGCGTTATGGCACAGGTTGGCTACGTGGATTTATAGCGGATTATGGAGTCCCACTCATGGTTCTTGTATGGACTGCTGTATCTTACATACCGGTTAATGATGTCCCAAAAGGAATCCCAAGACGACTTTTCAGTCCAAATCCATGGTCTCCTGGTGCATACTCAAACTGGACAGTTATCAAGGAGATGATGACTGTTCCTCCTTTATATATAGTTGGAGCATTTATACCAGCAACTATGATTGCTGTGCTCTACTACTTTGATCACAGTGTTGCATCTCAACTTGCCCAACAGAAGGAGTTTAATCTAAAGAAACCAGCTTCATATCACTATGACCTTCTCCTTCTTGGATTTCTGGTCATATTGTGTGGGCTTATTGGCATTCCCCCTGCCAATGGTGTAATTCCACAATCTCCTATGCATACAAAAAGTTTAGCTACTCTAAAACATCAGCTTTTGCGGAATAAGCTTGTAGTAACAGCAAGGAATAGTATACGCAAAAACTCAACTTTGAGTCAATTATACCACAACATGCAAGAAGCATACAATGAAATGCAGACTCCACTAGTCTACCAGCGTCCATCTGCTCTGGGACTAAAAGAGCTGAAAGAATCCACAGTCCAATTAGCCTCAAGTACTGGCTACATTGATGCCCCTGTTGATGAAACCGTTTTTGATGTGGATAAGGATATTGATGATCTTTTACCTATTGAAGTAAAAGAGCAACGCCTCAGCAATGTGCTTCAGGCATTGCTGGCTGGTGGTTGTGTAGCTGCCATGCCTCTTTTGAAGAAAATCCCAACCTCAGTTCTTTGGGGTTATTTTGCTTTTATGGCAATTGAAAGCTTGCCTGGAAACCAATTCTGGGAGAGAATATTGTTTCTTTTCACTGCTCCAAGTCGAAGATACAAGGTGCTGGAGAAGTATCATGGAACCTTTCTTGAGACTGTGCCTTTTAAAACAATTGCGACCTTCACCTTGTTCCAGACAGCTTACTTGCTTCTGTGTTTTGGCATAACATGGATCCCAATTGCCGGGGTGCTCTTCCCGTTGTTGATCATGCTTCTAGTCCCAGTGCGGCAGTATTTGCTCCCTAAGTTTTTTAAAAGGGCTCACCTTCAAGACCTGGATGCTGCAGAATACGAGGAAGCCCCTGCCATTGCTTTCAACATGTCCTTTGAAGATCAGGATGTTCAGGCAAGAATAAATAACATTGATGGTGGGGAGATACTTGATGAAATTATTACAAGAAGCCGCGGGGAGATCCGCCGCACTCAGAGTCCAAAAGTATCAAGTTTGACCCCTACTTCTTCACTAGAAGGTATAAAATCAACTTATAGCCCAAGGTTAACGCAGAGAGTATACAGCCCCCGTTTAAACGAACTGAGAGGAGAAAGAAGCCCCCGACTAAATGTACAAGGTCACGAAACAGAACACACTCCCAGCCCCCGTTTGAATGAACTAAGAGGAGAACAAAGCCCCCGAATGCCTGGTCAGGGAATCGAAACAGTGCAGACTCCCAGCCCTGGACCATCTGTCCTCGGACAAAGCACTCCTGGTTCTTCAAGCTACAAAACTAGGAGTGTGATCCAGGTGGATTATTTGGCAATGGTACTCGAGAGGTCATGTGCTAGGTTCATGAGTAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006873 GO:0006885 GO:0007275 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0022622 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0071944 GO:0098771 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

772

Amino Acids

86.41

Weight (kDa)

9.17

Isoelectric Point (pI)

45.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	7 - 180	1.6e-33	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	202 - 372	3.2e-24	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	460 - 602	1.3e-28	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47160 AT2G47160 AT3G62270
fragaria_vesca	FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400
malus_domestica	MD06G1173100.v1.1 MD09G1287700.v1.1 MD14G1179400.v1.1 MD17G1279900.v1.1
prunus_persica	Prupe.3G005600_v2.0.a1 Prupe.5G172600_v2.0.a1
pyrus_communis	pycom06g15420 pycom14g14930 pycom14g14940
rosa_chinensis	RchiOBHm_Chr3g0486761 RchiOBHm_Chr7g0193431
rosa_laevigata	RLG00000004268 RLG00000023062
rosa_multiflora	Rmu_sc0006629.1_g000001 Rmu_sc0012783.1_g000009 Rmu_sc0013001.1_g000011
rosa_roxburghii	Rroxscaffold_3G00262090 Rroxscaffold_6G00395610
rosa_rugosa	Rorug03G0227000 Rorug03G0227100 Rorug07G0009500
rosa_samantha	Rh3AG276900 Rh3BG311800 Rh3CG311000 Rh3DG307500 Rh7AG136100 Rh7BG136500 Rh7CG140000 Rh7DG137800
rosa_wichuraiana	Rw3G024620 Rw7G011610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	801
AccBSI	CCGCTC	1 cut(s)	95
AccI	GTMKAC	4 cut(s)	308, 1162, 1239, 2031
AccII	CGCG	3 cut(s)	27, 457, 1926
AciI	CCGC	9 cut(s)	27, 95, 707, 1129, 1747, 1924, 1926, 1936, 1939
AclI	AACGTT	1 cut(s)	11
AclWI	GGATC	6 cut(s)	38, 113, 1687, 1700, 1927, 2251
AcoI	YGGCCR	1 cut(s)	417
AcsI	RAATTY	2 cut(s)	54, 533
AcuI	CTGAAG	1 cut(s)	1397
AfaI	GTAC	5 cut(s)	207, 543, 1306, 2085, 2283
AfiI	CCNNNNNNNGG	8 cut(s)	26, 727, 964, 1064, 1534, 1703, 2165, 2195
AflIII	ACRYGT	2 cut(s)	455, 1839
AgeI	ACCGGT	1 cut(s)	760
AhdI	GACNNNNNGTC	1 cut(s)	2204
AhlI	ACTAGT	1 cut(s)	1234
AjnI	CCWGG	7 cut(s)	820, 1519, 1794, 2158, 2194, 2224, 2259
Alw21I	GWGCWC	2 cut(s)	921, 1713
Alw26I	GTCTC	2 cut(s)	822, 1607
AlwI	GGATC	6 cut(s)	38, 113, 1687, 1700, 1927, 2251
AlwNI	CAGNNNCTG	1 cut(s)	268
Ama87I	CYCGRG	1 cut(s)	2285
AoxI	GGCC	4 cut(s)	111, 417, 510, 628
ApeKI	GCWGC	5 cut(s)	169, 262, 505, 1442, 1802
ApoI	RAATTY	2 cut(s)	54, 533
ArsI	GACNNNNNNTTYG	6 cut(s)	385, 417, 800, 832, 2146, 2178
AsiGI	ACCGGT	1 cut(s)	760
AspS9I	GGNCC	3 cut(s)	112, 334, 2198
AsuC2I	CCSGG	1 cut(s)	1705
AsuHPI	GGTGA	3 cut(s)	479, 1636, 1775
AvaI	CYCGRG	1 cut(s)	2285
AvaII	GGWCC	2 cut(s)	334, 2198
BaeI	ACNNNNGTAYC	2 cut(s)	732, 765
BalI	TGGCCA	1 cut(s)	419
BamHI	GGATCC	1 cut(s)	1692
BanII	GRGCYC	1 cut(s)	1783
BbsI	GAAGAC	1 cut(s)	473
Bbv12I	GWGCWC	2 cut(s)	921, 1713
BbvCI	CCTCAGC	1 cut(s)	1400
BbvI	GCAGC	5 cut(s)	181, 274, 517, 1429, 1789
BccI	CCATC	4 cut(s)	372, 1258, 1880, 2209
BciT130I	CCWGG	7 cut(s)	822, 1521, 1796, 2160, 2196, 2226, 2261
BclI	TGATCA	2 cut(s)	931, 1725
BcnI	CCSGG	1 cut(s)	1705
BcoDI	GTCTC	2 cut(s)	822, 1607
BcuI	ACTAGT	1 cut(s)	1234
BfaI	CTAG	7 cut(s)	278, 663, 1235, 1736, 1985, 2247, 2301
BfmI	CTRYAG	2 cut(s)	201, 1803
BisI	GCNGC	8 cut(s)	170, 263, 506, 1443, 1748, 1803, 1924, 1939
BlsI	GCNGC	8 cut(s)	171, 264, 507, 1444, 1749, 1804, 1925, 1940
BmcAI	AGTACT	1 cut(s)	1306
Bme1390I	CCNGG	8 cut(s)	822, 1521, 1705, 1796, 2160, 2196, 2226, 2261
Bme18I	GGWCC	2 cut(s)	334, 2198
BmeRI	GACNNNNNGTC	1 cut(s)	2204
BmeT110I	CYCGRG	1 cut(s)	2285
BmgT120I	GGNCC	3 cut(s)	112, 334, 2198
BmiI	GGNNCC	3 cut(s)	114, 1603, 1694
BmrFI	CCNGG	8 cut(s)	822, 1521, 1705, 1796, 2160, 2196, 2226, 2261
BmrI	ACTGGG	1 cut(s)	1736
BmsI	GCATC	4 cut(s)	227, 953, 1309, 1789
BmuI	ACTGGG	1 cut(s)	1736
BpiI	GAAGAC	1 cut(s)	473
BplI	GAGNNNNNCTC	2 cut(s)	708, 740
BpmI	CTGGAG	1 cut(s)	1607
Bpu10I	CCTNAGC	2 cut(s)	330, 1400
BpuEI	CTTGAG	2 cut(s)	1285, 1631
BpuMI	CCSGG	1 cut(s)	1705
BsaAI	YACGTR	1 cut(s)	695
BsaI	GGTCTC	1 cut(s)	822
BsaJI	CCNNGG	6 cut(s)	812, 1704, 1924, 2012, 2194, 2209
BsaWI	WCCGGW	1 cut(s)	760
BsaXI	ACNNNNNCTCC	2 cut(s)	853, 883
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 727, 964, 1064, 1534, 1703, 2165, 2195
Bse118I	RCCGGY	1 cut(s)	760
Bse1I	ACTGG	5 cut(s)	149, 379, 842, 1312, 1742
Bse3DI	GCAATG	6 cut(s)	498, 604, 1411, 1418, 1827, 2283
BseBI	CCWGG	7 cut(s)	822, 1521, 1796, 2160, 2196, 2226, 2261
BseDI	CCNNGG	6 cut(s)	812, 1704, 1924, 2012, 2194, 2209
BseGI	GGATG	4 cut(s)	383, 395, 1804, 1864
BseLI	CCNNNNNNNGG	8 cut(s)	26, 727, 964, 1064, 1534, 1703, 2165, 2195
BseMI	GCAATG	6 cut(s)	498, 604, 1411, 1418, 1827, 2283
BseMII	CTCAG	4 cut(s)	1414, 1488, 1958, 2045
BseNI	ACTGG	5 cut(s)	149, 379, 842, 1312, 1742
BseRI	GAGGAG	3 cut(s)	534, 2073, 2151
BseXI	GCAGC	5 cut(s)	181, 274, 517, 1429, 1789
BseYI	CCCAGC	2 cut(s)	2111, 2189
BsgI	GTGCAG	2 cut(s)	555, 2201
Bsh1236I	CGCG	3 cut(s)	27, 457, 1926
BshFI	GGCC	4 cut(s)	113, 419, 512, 630
BshTI	ACCGGT	1 cut(s)	760
BsiHKAI	GWGCWC	2 cut(s)	921, 1713
BsiHKCI	CYCGRG	1 cut(s)	2285
BsiSI	CCGG	2 cut(s)	761, 1704
BslFI	GGGAC	5 cut(s)	363, 704, 758, 1275, 1724
BslI	CCNNNNNNNGG	8 cut(s)	26, 727, 964, 1064, 1534, 1703, 2165, 2195
BsmAI	GTCTC	2 cut(s)	822, 1607
BsmFI	GGGAC	5 cut(s)	363, 704, 758, 1275, 1724
BsmI	GAATGC	2 cut(s)	1050, 2160
BsnI	GGCC	4 cut(s)	113, 419, 512, 630
Bso31I	GGTCTC	1 cut(s)	822
BsoBI	CYCGRG	1 cut(s)	2285
Bsp1286I	GDGCHC	3 cut(s)	921, 1713, 1783
Bsp1407I	TGTACA	1 cut(s)	2083
Bsp19I	CCATGG	1 cut(s)	812
BspACI	CCGC	9 cut(s)	27, 95, 707, 1129, 1747, 1924, 1926, 1936, 1939
BspANI	GGCC	4 cut(s)	113, 419, 512, 630
BspCNI	CTCAG	4 cut(s)	1413, 1487, 1957, 2046
BspFNI	CGCG	3 cut(s)	27, 457, 1926
BspHI	TCATGA	1 cut(s)	2307
BspLI	GGNNCC	3 cut(s)	114, 1603, 1694
BspMAI	CTGCAG	2 cut(s)	205, 1807
BspPI	GGATC	6 cut(s)	38, 113, 1687, 1700, 1927, 2251
BspQI	GCTCTTC	2 cut(s)	413, 1718
BspTNI	GGTCTC	1 cut(s)	822
BsrBI	CCGCTC	1 cut(s)	95
BsrDI	GCAATG	6 cut(s)	498, 604, 1411, 1418, 1827, 2283
BsrFI	RCCGGY	1 cut(s)	760
BsrGI	TGTACA	1 cut(s)	2083
BsrI	ACTGG	5 cut(s)	149, 379, 842, 1312, 1742
BssAI	RCCGGY	1 cut(s)	760
BssECI	CCNNGG	6 cut(s)	812, 1704, 1924, 2012, 2194, 2209
BssNAI	GTATAC	3 cut(s)	309, 1163, 2032
BssT1I	CCWWGG	2 cut(s)	812, 2012
Bst1107I	GTATAC	3 cut(s)	309, 1163, 2032
Bst2UI	CCWGG	7 cut(s)	822, 1521, 1796, 2160, 2196, 2226, 2261
Bst4CI	ACNGT	8 cut(s)	298, 844, 865, 938, 1288, 1339, 1618, 2179
Bst6I	CTCTTC	2 cut(s)	413, 1718
BstAPI	GCANNNNNTGC	3 cut(s)	224, 268, 1756
BstAUI	TGTACA	1 cut(s)	2083
BstBAI	YACGTR	1 cut(s)	695
BstC8I	GCNNGC	5 cut(s)	417, 433, 510, 1425, 1517
BstDEI	CTNAG	7 cut(s)	330, 1400, 1474, 1764, 1944, 2054, 2132
BstDSI	CCRYGG	2 cut(s)	812, 1924
BstF5I	GGATG	4 cut(s)	383, 395, 1804, 1864
BstFNI	CGCG	3 cut(s)	27, 457, 1926
BstMAI	GTCTC	2 cut(s)	822, 1607
BstMWI	GCNNNNNNNGC	8 cut(s)	224, 268, 412, 502, 1135, 1424, 1502, 1756
BstNI	CCWGG	7 cut(s)	822, 1521, 1796, 2160, 2196, 2226, 2261
BstNSI	RCATGY	2 cut(s)	1204, 1843
BstSCI	CCNGG	8 cut(s)	820, 1519, 1703, 1794, 2158, 2194, 2224, 2259
BstSFI	CTRYAG	2 cut(s)	201, 1803
BstUI	CGCG	3 cut(s)	27, 457, 1926
BstV1I	GCAGC	5 cut(s)	181, 274, 517, 1429, 1789
BstV2I	GAAGAC	1 cut(s)	473
BstX2I	RGATCY	3 cut(s)	105, 1692, 1932
BstXI	CCANNNNNNTGG	1 cut(s)	813
BstYI	RGATCY	3 cut(s)	105, 1692, 1932
BstZ17I	GTATAC	3 cut(s)	309, 1163, 2032
BsuRI	GGCC	4 cut(s)	113, 419, 512, 630
BtgI	CCRYGG	2 cut(s)	812, 1924
BtsCI	GGATG	4 cut(s)	383, 395, 1804, 1864
BtsI	GCAGTG	1 cut(s)	1557
BtsIMutI	CAGTG	4 cut(s)	943, 1557, 1749, 2184
Cac8I	GCNNGC	5 cut(s)	417, 433, 510, 1425, 1517
CaiI	CAGNNNCTG	1 cut(s)	268
CciI	TCATGA	1 cut(s)	2307
Cfr10I	RCCGGY	1 cut(s)	760
Cfr13I	GGNCC	3 cut(s)	112, 334, 2198
Cfr42I	CCGCGG	1 cut(s)	1927
CseI	GACGC	1 cut(s)	1235
Csp6I	GTAC	5 cut(s)	206, 542, 1305, 2084, 2282
CspAI	ACCGGT	1 cut(s)	760
CviQI	GTAC	5 cut(s)	206, 542, 1305, 2084, 2282
DdeI	CTNAG	7 cut(s)	330, 1400, 1474, 1764, 1944, 2054, 2132
DraI	TTTAAA	3 cut(s)	1627, 1774, 2046
DrdI	GACNNNNNNGTC	1 cut(s)	801
DriI	GACNNNNNGTC	1 cut(s)	2204
DseDI	GACNNNNNNGTC	1 cut(s)	801
EaeI	YGGCCR	1 cut(s)	417
Eam1104I	CTCTTC	2 cut(s)	413, 1718
Eam1105I	GACNNNNNGTC	1 cut(s)	2204
EarI	CTCTTC	2 cut(s)	413, 1718
EciI	GGCGGA	1 cut(s)	1925
Eco130I	CCWWGG	2 cut(s)	812, 2012
Eco24I	GRGCYC	1 cut(s)	1783
Eco31I	GGTCTC	1 cut(s)	822
Eco47I	GGWCC	2 cut(s)	334, 2198
Eco57I	CTGAAG	1 cut(s)	1397
Eco88I	CYCGRG	1 cut(s)	2285
EcoRI	GAATTC	1 cut(s)	54
EcoRII	CCWGG	7 cut(s)	820, 1519, 1794, 2158, 2194, 2224, 2259
EcoT14I	CCWWGG	2 cut(s)	812, 2012
EcoT22I	ATGCAT	1 cut(s)	1092
EcoT38I	GRGCYC	1 cut(s)	1783
ErhI	CCWWGG	2 cut(s)	812, 2012
FaqI	GGGAC	5 cut(s)	363, 704, 758, 1275, 1724
FauI	CCCGC	3 cut(s)	20, 88, 1919
FauNDI	CATATG	1 cut(s)	124
FbaI	TGATCA	2 cut(s)	931, 1725
FblI	GTMKAC	4 cut(s)	308, 1162, 1239, 2031
Fnu4HI	GCNGC	8 cut(s)	170, 263, 506, 1443, 1748, 1803, 1924, 1939
FokI	GGATG	4 cut(s)	382, 390, 1811, 1871
FriOI	GRGCYC	1 cut(s)	1783
Fsp4HI	GCNGC	8 cut(s)	170, 263, 506, 1443, 1748, 1803, 1924, 1939
FspBI	CTAG	7 cut(s)	278, 663, 1235, 1736, 1985, 2247, 2301
GluI	GCNGC	8 cut(s)	170, 263, 506, 1443, 1748, 1803, 1924, 1939
GsaI	CCCAGC	2 cut(s)	2115, 2193
GsuI	CTGGAG	1 cut(s)	1607
HaeIII	GGCC	4 cut(s)	113, 419, 512, 630
HapII	CCGG	2 cut(s)	761, 1704
HgaI	GACGC	1 cut(s)	1235
HincII	GTYRAC	1 cut(s)	2019
HindII	GTYRAC	1 cut(s)	2019
HindIII	AAGCTT	2 cut(s)	1136, 1513
HinfI	GANTC	9 cut(s)	211, 717, 783, 1183, 1228, 1280, 1948, 2169, 2185
HpaI	GTTAAC	1 cut(s)	2019
HpaII	CCGG	2 cut(s)	761, 1704
HphI	GGTGA	3 cut(s)	479, 1636, 1775
Hpy166II	GTNNAC	8 cut(s)	89, 309, 393, 453, 1163, 1240, 2019, 2032
Hpy188I	TCNGA	3 cut(s)	567, 1947, 2213
Hpy188III	TCNNGA	7 cut(s)	1610, 1654, 1790, 1856, 2093, 2287, 2308
Hpy8I	GTNNAC	8 cut(s)	89, 309, 393, 453, 1163, 1240, 2019, 2032
HpyAV	CCTTC	8 cut(s)	591, 641, 958, 1016, 1022, 1651, 1796, 1982
HpyCH4III	ACNGT	8 cut(s)	298, 844, 865, 938, 1288, 1339, 1618, 2179
HpyCH4IV	ACGT	2 cut(s)	11, 694
HpyF10VI	GCNNNNNNNGC	8 cut(s)	224, 268, 412, 502, 1135, 1424, 1502, 1756
HpyF3I	CTNAG	7 cut(s)	330, 1400, 1474, 1764, 1944, 2054, 2132
HpySE526I	ACGT	2 cut(s)	11, 694
Ksp22I	TGATCA	2 cut(s)	931, 1725
KspAI	GTTAAC	1 cut(s)	2019
KspI	CCGCGG	1 cut(s)	1927
LguI	GCTCTTC	2 cut(s)	413, 1718
LmnI	GCTCC	4 cut(s)	166, 887, 1567, 1764
Lsp1109I	GCAGC	5 cut(s)	181, 274, 517, 1429, 1789
LweI	GCATC	4 cut(s)	227, 953, 1309, 1789
MaeI	CTAG	7 cut(s)	278, 663, 1235, 1736, 1985, 2247, 2301
MaeII	ACGT	2 cut(s)	11, 694
MaeIII	GTNAC	3 cut(s)	1144, 2090, 2312
MbiI	CCGCTC	1 cut(s)	95
MfeI	CAATTG	4 cut(s)	249, 1506, 1632, 1698
MflI	RGATCY	3 cut(s)	105, 1692, 1932
MhlI	GDGCHC	3 cut(s)	921, 1713, 1783
MlsI	TGGCCA	1 cut(s)	419
MluI	ACGCGT	1 cut(s)	455
MluNI	TGGCCA	1 cut(s)	419
MlyI	GAGTC	6 cut(s)	205, 726, 1192, 1222, 1957, 2179
MmeI	TCCRAC	2 cut(s)	235, 865
Mox20I	TGGCCA	1 cut(s)	419
Mph1103I	ATGCAT	1 cut(s)	1092
MscI	TGGCCA	1 cut(s)	419
MseI	TTAA	6 cut(s)	765, 972, 1626, 1773, 2018, 2045
Msp20I	TGGCCA	1 cut(s)	419
MspA1I	CMGCKG	2 cut(s)	172, 1926
MspI	CCGG	2 cut(s)	761, 1704
MspR9I	CCNGG	8 cut(s)	822, 1521, 1705, 1796, 2160, 2196, 2226, 2261
MssI	GTTTAAAC	1 cut(s)	2046
MunI	CAATTG	4 cut(s)	249, 1506, 1632, 1698
Mva1269I	GAATGC	2 cut(s)	1050, 2160
MvaI	CCWGG	7 cut(s)	822, 1521, 1796, 2160, 2196, 2226, 2261
MvnI	CGCG	3 cut(s)	27, 457, 1926
MwoI	GCNNNNNNNGC	8 cut(s)	224, 268, 412, 502, 1135, 1424, 1502, 1756
NciI	CCSGG	1 cut(s)	1705
NcoI	CCATGG	1 cut(s)	812
NdeI	CATATG	1 cut(s)	124
NlaIV	GGNNCC	3 cut(s)	114, 1603, 1694
NmuCI	GTSAC	1 cut(s)	2090
NsiI	ATGCAT	1 cut(s)	1092
NspI	RCATGY	2 cut(s)	1204, 1843
PaeR7I	CTCGAG	1 cut(s)	2285
PagI	TCATGA	1 cut(s)	2307
PciI	ACATGT	1 cut(s)	1839
PciSI	GCTCTTC	2 cut(s)	413, 1718
PctI	GAATGC	2 cut(s)	1050, 2160
PfeI	GAWTC	3 cut(s)	783, 1280, 2169
PinAI	ACCGGT	1 cut(s)	760
PkrI	GCNGC	8 cut(s)	171, 264, 507, 1444, 1749, 1804, 1925, 1940
PleI	GAGTC	6 cut(s)	205, 725, 1191, 1222, 1956, 2179
PmeI	GTTTAAAC	1 cut(s)	2046
PpsI	GAGTC	6 cut(s)	205, 725, 1191, 1222, 1956, 2179
Ppu21I	YACGTR	1 cut(s)	695
PscI	ACATGT	1 cut(s)	1839
Psp1406I	AACGTT	1 cut(s)	11
Psp6I	CCWGG	7 cut(s)	820, 1519, 1794, 2158, 2194, 2224, 2259
PspFI	CCCAGC	2 cut(s)	2111, 2189
PspGI	CCWGG	7 cut(s)	820, 1519, 1794, 2158, 2194, 2224, 2259
PspN4I	GGNNCC	3 cut(s)	114, 1603, 1694
PspPI	GGNCC	3 cut(s)	112, 334, 2198
PstI	CTGCAG	2 cut(s)	205, 1807
PstNI	CAGNNNCTG	1 cut(s)	268
PsuI	RGATCY	3 cut(s)	105, 1692, 1932
PvuII	CAGCTG	1 cut(s)	172
RsaI	GTAC	5 cut(s)	207, 543, 1306, 2085, 2283
RsaNI	GTAC	5 cut(s)	206, 542, 1305, 2084, 2282
SacII	CCGCGG	1 cut(s)	1927
SapI	GCTCTTC	2 cut(s)	413, 1718
SaqAI	TTAA	6 cut(s)	765, 972, 1626, 1773, 2018, 2045
SatI	GCNGC	8 cut(s)	170, 263, 506, 1443, 1748, 1803, 1924, 1939
Sau96I	GGNCC	3 cut(s)	112, 334, 2198
ScaI	AGTACT	1 cut(s)	1306
SchI	GAGTC	6 cut(s)	205, 726, 1192, 1222, 1957, 2179
ScrFI	CCNGG	8 cut(s)	822, 1521, 1705, 1796, 2160, 2196, 2226, 2261
SduI	GDGCHC	3 cut(s)	921, 1713, 1783
SfaNI	GCATC	4 cut(s)	227, 953, 1309, 1789
SfcI	CTRYAG	2 cut(s)	201, 1803
Sfr274I	CTCGAG	1 cut(s)	2285
Sfr303I	CCGCGG	1 cut(s)	1927
SgrBI	CCGCGG	1 cut(s)	1927
SinI	GGWCC	2 cut(s)	334, 2198
SlaI	CTCGAG	1 cut(s)	2285
SmlI	CTYRAG	3 cut(s)	1300, 1610, 2285
SmoI	CTYRAG	3 cut(s)	1300, 1610, 2285
SpeI	ACTAGT	1 cut(s)	1234
SsiI	CCGC	9 cut(s)	27, 95, 707, 1129, 1747, 1924, 1926, 1936, 1939
SspI	AATATT	1 cut(s)	1545
SspMI	CTAG	7 cut(s)	278, 663, 1235, 1736, 1985, 2247, 2301
StyD4I	CCNGG	8 cut(s)	820, 1519, 1703, 1794, 2158, 2194, 2224, 2259
StyI	CCWWGG	2 cut(s)	812, 2012
TaaI	ACNGT	8 cut(s)	298, 844, 865, 938, 1288, 1339, 1618, 2179
TaiI	ACGT	2 cut(s)	14, 697
TaqI	TCGA	3 cut(s)	1570, 2172, 2286
TaqII	GACCGA	1 cut(s)	351
TatI	WGTACW	3 cut(s)	205, 1304, 2083
TauI	GCSGC	3 cut(s)	1750, 1926, 1941
TfiI	GAWTC	3 cut(s)	783, 1280, 2169
Tru1I	TTAA	6 cut(s)	765, 972, 1626, 1773, 2018, 2045
Tru9I	TTAA	6 cut(s)	765, 972, 1626, 1773, 2018, 2045
TscAI	CASTG	4 cut(s)	943, 1564, 1749, 2184
TseFI	GTSAC	1 cut(s)	2090
TseI	GCWGC	5 cut(s)	169, 262, 505, 1442, 1802
Tsp45I	GTSAC	1 cut(s)	2090
TspDTI	ATGAA	6 cut(s)	981, 1233, 1347, 1920, 2142, 2296
TspRI	CASTG	4 cut(s)	943, 1564, 1749, 2184
VpaK11BI	GGWCC	2 cut(s)	334, 2198
XapI	RAATTY	2 cut(s)	54, 533
XceI	RCATGY	2 cut(s)	1204, 1843
XcmI	CCANNNNNNNNNTGG	1 cut(s)	251
XhoI	CTCGAG	1 cut(s)	2285
XmiI	GTMKAC	4 cut(s)	308, 1162, 1239, 2031
XspI	CTAG	7 cut(s)	278, 663, 1235, 1736, 1985, 2247, 2301
ZrmI	AGTACT	1 cut(s)	1306
Zsp2I	ATGCAT	1 cut(s)	1092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.