Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001955

Physical Location & Seq

Genomic Coordinates Reverse (-)

36758275 .. 36763931

Gene Structure 5657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1287700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGAAGAGACATTTGTGCCTTTGCGGGGAATCAAGAATGATCTCAGAGGAAGATTGAAGTGCTACAAGGAAGACTGGACTGGGGGCTTTAAAGCTGGATTCAGGATTTTGGCTCCTACAACCTACATATTTTTTGCTTCTGCAATCCCAGTCATTTCATTTGGTGAACAATTGGATCGAAGTACTGATGGAGTTTTGACAGCAGTGCAAACCTTAGTATCGACGGCAGTTTGTGGGATCATACATTCCATCGTCGGAGGTCAACCCTTGCTGATATTGGGTGTGGCCGAGCCTACTGTAATCATGTACACCTTCATGTTCAATTTTGCCAAAGAACGTAAGGACTTGGGTCCAAAGCTCTTTCTAGCATGGTCCGCATGGGTATGTGTATGGACAGCTGGTTTACTGTTCTTATTGGCAATATTAGGCGCTTGTTCCATAATAAATAGGTTTACAAGAGTGGCTGGGGAGTTGTTTGGTCTACTTATTGCTATGCTCTTCATGCAGCAAGCCATTAAAGGACTTGTGGATGAGTTTCGCTTACCAGAAAGAGAAGACACTAGTTTACTACAATTTATACCTTCATGGAGGTTTGCAAATGGAATGTTTGCTTTGGTGTTGTCATTTGGCCTTCTTCTCACAGCATTAAAAAGCAGGAAAGCAAGGTCATGGCGCTATGGCACTGGTTGGCTAAGAAGCTTTGTAGCAGACTATGGTGTGCCACTTATGGTTCTGGTGTGGACTGGTGTGTCTTACATTCCAACTAGTAGTGTTCCACACGGCATCCCAAGGCGCCTTTTTAGCCCAAATCCATGGTCACCTGGTGCATATGAAAATTGGACTGTCATTAAGGACATGCTCAATGTTCCAGTCCTCTATATCATTGGAGCTTTCATTCCAGCAACGATGATCGCGGTGCTTTACTATTTTGATCACAGTGTAGCATCCCAACTATCTCAGCAGAAAGAGTTCAACTTAAGAAAGCCATCCTCTTACCATTATGACTTGCTTCTTTTGGGGTTTCTGACTTTAATGTGTGGTCTGCTTGGAATTCCTCCATCAAATGGAGTCATCCCACAGTCTCCAATGCATACAAAAAGTTTAGCTACTCTTAAACACCAGTTGCTTCGTAATCGTCTTGTAGCAACAGCACGCACAAGTATGAGAAATAATGCTAGCTTGGGACAACTGTATGGAAATATGCAAGATGCCTATCAACAAATGCAGACTCCTTTGGTTTACCAGGAGGCCTCATCTCGAGGACTGAATGAATTAAAAGAATCAACCATCCAGGCAGCTTCGAGTATGGGAAATTATATTGATGCACCAGTTGATCAGACAGTATTTGATATTGAGAAAGAGATTGATGATCTATTGCCGGTTGAGGTGAAGGAGCAGCGTCTCAGTAACCTGCTTCAAGCGGTATTGGTGGGGGGATGTGTTGCAGCCATGCCTATCCTCAAAAGAATCCCAACTTCAGTCCTTTGGGGATACTTTGCCTTCATGGCCATTGAAAGCTTACCCGGTAACCAATTTTGGGAAAGGATTTTATTGCTCTTTACAGCTCCAAGTAGAAGATTCAAAGTCCTCGAGGATAATCACGCCACTTTTGTAGAAACTGTGCCTTTCAAGTCAATCGCGATGTTCACAGTTTTCCAAACGATGTACCTGCTTGCATGTTTTGGGCTTACTTGGGTTCCAATTGCTGGGGTTATGTTTCCAATGATGATCATGCTCTTGGTTCCTGTGAGGCAATACATACTCCCCAAGTTTTTCAAAGGAGCACACCTTCAGGATTTGGATGCAGCAGAGTACGAAGAGGCTCCGGCTTTAACATATAACCTTGCAACGGAGACAGAGTTGGGAGCTGGAGCTTCCTGCGCAGGGGATGCAGAAATATTAGACGAAGTTATGACCAGAAGCCGAGGTGAGTTTAGGCATGTCAGCAGTCCAAAGATCACAAGCTCTACTTCAACACCAGCAAATGCCCCTGAAATCCTTGGGAGTCCACACAAGACCTTCAGTCCTCGCGTAAGTGAGCTAAGAGGAGAGCGAAGTCCTCTTTCTGGCGGAAGAGGGTCGCACAGTCCGGGGACCCCTGGCTCTTCTTCTATTCTTGGGAAGAGTCCTAGTAACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006873 GO:0006885 GO:0007275 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0022622 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0071944 GO:0098771 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

714

Amino Acids

78.88

Weight (kDa)

8.68

Isoelectric Point (pI)

44.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	8 - 180	2.8e-34	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	201 - 372	4.4e-24	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	461 - 603	1.5e-30	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47160 AT2G47160 AT3G62270
fragaria_vesca	FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_1g26620 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400 FvH4_5g05400
malus_domestica	MD06G1173100.v1.1 MD09G1287700.v1.1 MD14G1179400.v1.1 MD17G1279900.v1.1
prunus_persica	Prupe.3G005600_v2.0.a1 Prupe.5G172600_v2.0.a1
pyrus_communis	pycom06g15420 pycom14g14930 pycom14g14940
rosa_chinensis	RchiOBHm_Chr3g0486761 RchiOBHm_Chr7g0193431
rosa_laevigata	RLG00000004268 RLG00000023062
rosa_multiflora	Rmu_sc0006629.1_g000001 Rmu_sc0012783.1_g000009 Rmu_sc0013001.1_g000011
rosa_roxburghii	Rroxscaffold_3G00262090 Rroxscaffold_6G00395610
rosa_rugosa	Rorug03G0227000 Rorug03G0227100 Rorug07G0009500
rosa_samantha	Rh3AG276900 Rh3BG311800 Rh3CG311000 Rh3DG307500 Rh7AG136100 Rh7BG136500 Rh7CG140000 Rh7DG137800
rosa_wichuraiana	Rw3G024620 Rw7G011610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	748
AbsI	CCTCGAGG	1 cut(s)	1589
Acc16I	TGCGCA	1 cut(s)	1882
Acc36I	ACCTGC	2 cut(s)	1419, 1677
AccB1I	GGYRCC	1 cut(s)	792
AccB7I	CCANNNNNTGG	2 cut(s)	1064, 1706
AccI	GTMKAC	1 cut(s)	481
AccII	CGCG	3 cut(s)	914, 1640, 2031
AciI	CCGC	5 cut(s)	25, 375, 914, 1421, 2070
AclWI	GGATC	2 cut(s)	183, 245
AcoI	YGGCCR	2 cut(s)	285, 1506
AcsI	RAATTY	1 cut(s)	1050
AcuI	CTGAAG	3 cut(s)	1461, 1775, 2005
AcyI	GRCGYC	1 cut(s)	793
AdeI	CACNNNGTG	1 cut(s)	824
AfaI	GTAC	4 cut(s)	184, 308, 1667, 1814
AfiI	CCNNNNNNNGG	7 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 2066
AflII	CTTAAG	1 cut(s)	976
AgsI	TTSAA	9 cut(s)	58, 322, 973, 1418, 1514, 1582, 1630, 1777, 1974
AhdI	GACNNNNNGTC	1 cut(s)	2022
AhlI	ACTAGT	2 cut(s)	560, 764
AjnI	CCWGG	4 cut(s)	820, 1242, 1290, 2098
Alw21I	GWGCWC	1 cut(s)	1786
Alw26I	GTCTC	4 cut(s)	2, 1086, 1406, 1847
AlwI	GGATC	2 cut(s)	183, 245
Ama87I	CYCGRG	2 cut(s)	1257, 1589
AoxI	GGCC	4 cut(s)	285, 628, 1248, 1506
ApeKI	GCWGC	5 cut(s)	505, 1295, 1396, 1445, 1805
ApoI	RAATTY	1 cut(s)	1050
ArsI	GACNNNNNNTTYG	2 cut(s)	800, 832
Asp700I	GAANNNNTTC	1 cut(s)	968
AspLEI	GCGC	4 cut(s)	431, 675, 795, 1883
AspS9I	GGNCC	3 cut(s)	350, 372, 2094
AsuC2I	CCSGG	2 cut(s)	1524, 2091
AsuHPI	GGTGA	4 cut(s)	176, 810, 1399, 1940
AsuNHI	GCTAGC	1 cut(s)	1175
AvaI	CYCGRG	2 cut(s)	1257, 1589
AvaII	GGWCC	3 cut(s)	350, 372, 2094
BaeI	ACNNNNGTAYC	2 cut(s)	201, 234
BalI	TGGCCA	1 cut(s)	1508
BanI	GGYRCC	1 cut(s)	792
BbsI	GAAGAC	2 cut(s)	78, 561
Bbv12I	GWGCWC	1 cut(s)	1786
BbvI	GCAGC	5 cut(s)	517, 1307, 1408, 1457, 1817
BccI	CCATC	5 cut(s)	182, 257, 994, 1066, 1295
BceAI	ACGGC	2 cut(s)	240, 796
BcgI	CGANNNNNNTGC	2 cut(s)	1651, 1685
BciT130I	CCWGG	4 cut(s)	822, 1244, 1292, 2100
BciVI	GTATCC	1 cut(s)	1484
BclI	TGATCA	3 cut(s)	931, 1333, 1728
BcnI	CCSGG	2 cut(s)	1524, 2091
BcoDI	GTCTC	4 cut(s)	2, 1086, 1406, 1847
BcuI	ACTAGT	2 cut(s)	560, 764
BfaI	CTAG	5 cut(s)	365, 561, 765, 1176, 2130
BfoI	RGCGCY	3 cut(s)	432, 676, 796
BfrI	CTTAAG	1 cut(s)	976
BfuAI	ACCTGC	2 cut(s)	1419, 1677
BfuI	GTATCC	1 cut(s)	1484
BglI	GCCNNNNNGGC	1 cut(s)	1505
BisI	GCNGC	5 cut(s)	506, 1296, 1397, 1446, 1806
BlsI	GCNGC	5 cut(s)	507, 1297, 1398, 1447, 1807
BmcAI	AGTACT	1 cut(s)	184
Bme1390I	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 2091, 2100
Bme18I	GGWCC	3 cut(s)	350, 372, 2094
BmeRI	GACNNNNNGTC	1 cut(s)	2022
BmeT110I	CYCGRG	2 cut(s)	1257, 1589
BmgT120I	GGNCC	3 cut(s)	350, 372, 2094
BmiI	GGNNCC	8 cut(s)	114, 351, 794, 1698, 1743, 1824, 2095, 2096
BmrFI	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 2091, 2100
BmrI	ACTGGG	2 cut(s)	90, 143
BmsI	GCATC	6 cut(s)	792, 953, 1198, 1312, 1792, 1879
BmtI	GCTAGC	1 cut(s)	1179
BmuI	ACTGGG	2 cut(s)	90, 143
BoxI	GACNNNNGTC	1 cut(s)	348
BpiI	GAAGAC	2 cut(s)	78, 561
BpmI	CTGGAG	1 cut(s)	1890
BpuMI	CCSGG	2 cut(s)	1524, 2091
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaHI	GRCGYC	1 cut(s)	793
BsaJI	CCNNGG	6 cut(s)	788, 812, 1924, 1999, 2090, 2098
BsaXI	ACNNNNNCTCC	2 cut(s)	1844, 1874
Bsc4I	CCNNNNNNNGG	7 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 2066
Bse118I	RCCGGY	1 cut(s)	1378
Bse1I	ACTGG	8 cut(s)	80, 85, 149, 688, 748, 869, 1120, 1328
Bse8I	GATNNNNATC	1 cut(s)	1212
BseBI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
BseDI	CCNNGG	6 cut(s)	788, 812, 1924, 1999, 2090, 2098
BseGI	GGATG	9 cut(s)	535, 783, 944, 986, 1071, 1287, 1442, 1807, 1894
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	7 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 2066
BseMII	CTCAG	3 cut(s)	58, 971, 1417
BseNI	ACTGG	8 cut(s)	80, 85, 149, 688, 748, 869, 1120, 1328
BseRI	GAGGAG	1 cut(s)	2061
BseXI	GCAGC	5 cut(s)	517, 1307, 1408, 1457, 1817
BseYI	CCCAGC	2 cut(s)	464, 1706
Bsh1236I	CGCG	3 cut(s)	914, 1640, 2031
BshFI	GGCC	4 cut(s)	287, 630, 1250, 1508
BshNI	GGYRCC	1 cut(s)	792
BsiHKAI	GWGCWC	1 cut(s)	1786
BsiHKCI	CYCGRG	2 cut(s)	1257, 1589
BsiSI	CCGG	4 cut(s)	1379, 1524, 1826, 2090
BslFI	GGGAC	2 cut(s)	1197, 2107
BslI	CCNNNNNNNGG	7 cut(s)	26, 1064, 1537, 1706, 1849, 1884, 2066
BsmAI	GTCTC	4 cut(s)	2, 1086, 1406, 1847
BsmBI	CGTCTC	1 cut(s)	1406
BsmFI	GGGAC	2 cut(s)	1197, 2107
BsnI	GGCC	4 cut(s)	287, 630, 1250, 1508
BsoBI	CYCGRG	2 cut(s)	1257, 1589
Bsp1286I	GDGCHC	1 cut(s)	1786
Bsp1407I	TGTACA	1 cut(s)	306
Bsp143I	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
Bsp19I	CCATGG	1 cut(s)	812
Bsp68I	TCGCGA	1 cut(s)	1640
BspACI	CCGC	5 cut(s)	25, 375, 914, 1421, 2070
BspANI	GGCC	4 cut(s)	287, 630, 1250, 1508
BspCNI	CTCAG	3 cut(s)	57, 970, 1416
BspFNI	CGCG	3 cut(s)	914, 1640, 2031
BspLI	GGNNCC	8 cut(s)	114, 351, 794, 1698, 1743, 1824, 2095, 2096
BspMI	ACCTGC	2 cut(s)	1419, 1677
BspOI	GCTAGC	1 cut(s)	1179
BspPI	GGATC	2 cut(s)	183, 245
BspQI	GCTCTTC	2 cut(s)	503, 2110
BspT107I	GGYRCC	1 cut(s)	792
BspTI	CTTAAG	1 cut(s)	976
BsrFI	RCCGGY	1 cut(s)	1378
BsrGI	TGTACA	1 cut(s)	306
BsrI	ACTGG	8 cut(s)	80, 85, 149, 688, 748, 869, 1120, 1328
BssAI	RCCGGY	1 cut(s)	1378
BssECI	CCNNGG	6 cut(s)	788, 812, 1924, 1999, 2090, 2098
BssMI	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
BssNI	GRCGYC	1 cut(s)	793
BssT1I	CCWWGG	3 cut(s)	788, 812, 1999
Bst2UI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
Bst6I	CTCTTC	5 cut(s)	503, 1812, 2068, 2110, 2117
BstACI	GRCGYC	1 cut(s)	793
BstAFI	CTTAAG	1 cut(s)	976
BstAPI	GCANNNNNTGC	1 cut(s)	1889
BstAUI	TGTACA	1 cut(s)	306
BstC8I	GCNNGC	4 cut(s)	510, 1153, 1177, 1674
BstDEI	CTNAG	6 cut(s)	44, 214, 692, 957, 1403, 2042
BstDSI	CCRYGG	1 cut(s)	812
BstEII	GGTNACC	2 cut(s)	816, 1526
BstF5I	GGATG	9 cut(s)	535, 783, 944, 986, 1071, 1287, 1442, 1807, 1894
BstFNI	CGCG	3 cut(s)	914, 1640, 2031
BstH2I	RGCGCY	3 cut(s)	432, 676, 796
BstHHI	GCGC	4 cut(s)	431, 675, 795, 1883
BstKTI	GATC	9 cut(s)	43, 178, 240, 912, 934, 1336, 1372, 1731, 1959
BstMAI	GTCTC	4 cut(s)	2, 1086, 1406, 1847
BstMBI	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
BstMWI	GCNNNNNNNGC	6 cut(s)	374, 502, 801, 1505, 1880, 1889
BstNI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
BstNSI	RCATGY	3 cut(s)	859, 1680, 1943
BstPAI	GACNNNNGTC	1 cut(s)	348
BstPI	GGTNACC	2 cut(s)	816, 1526
BstSCI	CCNGG	6 cut(s)	820, 1242, 1290, 1522, 2089, 2098
BstUI	CGCG	3 cut(s)	914, 1640, 2031
BstV1I	GCAGC	5 cut(s)	517, 1307, 1408, 1457, 1817
BstV2I	GAAGAC	2 cut(s)	78, 561
BstXI	CCANNNNNNTGG	1 cut(s)	813
BsuI	GTATCC	1 cut(s)	1484
BsuRI	GGCC	4 cut(s)	287, 630, 1250, 1508
BtgI	CCRYGG	1 cut(s)	812
BtgZI	GCGATG	1 cut(s)	1655
BtsCI	GGATG	9 cut(s)	535, 783, 944, 986, 1071, 1287, 1442, 1807, 1894
BtsI	GCAGTG	1 cut(s)	210
BtsIMutI	CAGTG	3 cut(s)	210, 681, 943
BtuMI	TCGCGA	1 cut(s)	1640
BveI	ACCTGC	2 cut(s)	1419, 1677
Cac8I	GCNNGC	4 cut(s)	510, 1153, 1177, 1674
CfoI	GCGC	4 cut(s)	431, 675, 795, 1883
Cfr10I	RCCGGY	1 cut(s)	1378
Cfr13I	GGNCC	3 cut(s)	350, 372, 2094
CseI	GACGC	1 cut(s)	1388
CsiI	ACCWGGT	1 cut(s)	820
Csp6I	GTAC	4 cut(s)	183, 307, 1666, 1813
CviQI	GTAC	4 cut(s)	183, 307, 1666, 1813
DdeI	CTNAG	6 cut(s)	44, 214, 692, 957, 1403, 2042
DinI	GGCGCC	1 cut(s)	794
DpnI	GATC	9 cut(s)	42, 177, 239, 911, 933, 1335, 1371, 1730, 1958
DpnII	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
DraI	TTTAAA	1 cut(s)	91
DraIII	CACNNNGTG	1 cut(s)	824
DrdI	GACNNNNNNGTC	1 cut(s)	748
DriI	GACNNNNNGTC	1 cut(s)	2022
DseDI	GACNNNNNNGTC	1 cut(s)	748
EaeI	YGGCCR	2 cut(s)	285, 1506
Eam1104I	CTCTTC	5 cut(s)	503, 1812, 2068, 2110, 2117
Eam1105I	GACNNNNNGTC	1 cut(s)	2022
EarI	CTCTTC	5 cut(s)	503, 1812, 2068, 2110, 2117
EciI	GGCGGA	1 cut(s)	2085
Eco130I	CCWWGG	3 cut(s)	788, 812, 1999
Eco147I	AGGCCT	1 cut(s)	1250
Eco47I	GGWCC	3 cut(s)	350, 372, 2094
Eco57I	CTGAAG	3 cut(s)	1461, 1775, 2005
Eco88I	CYCGRG	2 cut(s)	1257, 1589
Eco91I	GGTNACC	2 cut(s)	816, 1526
EcoO109I	RGGNCCY	1 cut(s)	2094
EcoO65I	GGTNACC	2 cut(s)	816, 1526
EcoRI	GAATTC	1 cut(s)	1050
EcoRII	CCWGG	4 cut(s)	820, 1242, 1290, 2098
EcoT14I	CCWWGG	3 cut(s)	788, 812, 1999
EcoT22I	ATGCAT	1 cut(s)	1092
EgeI	GGCGCC	1 cut(s)	794
EheI	GGCGCC	1 cut(s)	794
ErhI	CCWWGG	3 cut(s)	788, 812, 1999
Esp3I	CGTCTC	1 cut(s)	1406
FalI	AAGNNNNNCTT	2 cut(s)	1954, 1986
FaqI	GGGAC	2 cut(s)	1197, 2107
FauI	CCCGC	1 cut(s)	18
FauNDI	CATATG	1 cut(s)	829
FbaI	TGATCA	3 cut(s)	931, 1333, 1728
FblI	GTMKAC	1 cut(s)	481
Fnu4HI	GCNGC	5 cut(s)	506, 1296, 1397, 1446, 1806
FokI	GGATG	9 cut(s)	542, 770, 931, 973, 1058, 1274, 1449, 1814, 1901
Fsp4HI	GCNGC	5 cut(s)	506, 1296, 1397, 1446, 1806
FspBI	CTAG	5 cut(s)	365, 561, 765, 1176, 2130
FspI	TGCGCA	1 cut(s)	1882
GlaI	GCGC	4 cut(s)	430, 674, 794, 1882
GluI	GCNGC	5 cut(s)	506, 1296, 1397, 1446, 1806
GsaI	CCCAGC	2 cut(s)	468, 1710
GsuI	CTGGAG	1 cut(s)	1890
HaeII	RGCGCY	3 cut(s)	432, 676, 796
HaeIII	GGCC	4 cut(s)	287, 630, 1250, 1508
HapII	CCGG	4 cut(s)	1379, 1524, 1826, 2090
HgaI	GACGC	1 cut(s)	1388
HhaI	GCGC	4 cut(s)	431, 675, 795, 1883
Hin1I	GRCGYC	1 cut(s)	793
Hin6I	GCGC	4 cut(s)	429, 673, 793, 1881
HinP1I	GCGC	4 cut(s)	429, 673, 793, 1881
HincII	GTYRAC	1 cut(s)	263
HindII	GTYRAC	1 cut(s)	263
HindIII	AAGCTT	2 cut(s)	697, 1516
HinfI	GANTC	9 cut(s)	30, 99, 1068, 1228, 1280, 1467, 1578, 2005, 2125
HpaII	CCGG	4 cut(s)	1379, 1524, 1826, 2090
HphI	GGTGA	4 cut(s)	176, 810, 1399, 1940
Hpy188I	TCNGA	4 cut(s)	47, 257, 1026, 1338
Hpy188III	TCNNGA	5 cut(s)	34, 103, 1257, 1639, 1793
Hpy99I	CGWCG	2 cut(s)	226, 257
HpyAV	CCTTC	7 cut(s)	322, 591, 641, 1384, 1510, 1799, 2029
HpyCH4IV	ACGT	1 cut(s)	337
HpyF10VI	GCNNNNNNNGC	6 cut(s)	374, 502, 801, 1505, 1880, 1889
HpyF3I	CTNAG	6 cut(s)	44, 214, 692, 957, 1403, 2042
HpySE526I	ACGT	1 cut(s)	337
Hsp92I	GRCGYC	1 cut(s)	793
HspAI	GCGC	4 cut(s)	429, 673, 793, 1881
KasI	GGCGCC	1 cut(s)	792
KflI	GGGWCCC	1 cut(s)	2094
Ksp22I	TGATCA	3 cut(s)	931, 1333, 1728
Kzo9I	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
LguI	GCTCTTC	2 cut(s)	503, 2110
LmnI	GCTCC	8 cut(s)	118, 887, 1393, 1570, 1781, 1828, 1865, 1871
Lsp1109I	GCAGC	5 cut(s)	517, 1307, 1408, 1457, 1817
LweI	GCATC	6 cut(s)	792, 953, 1198, 1312, 1792, 1879
MabI	ACCWGGT	1 cut(s)	820
MaeI	CTAG	5 cut(s)	365, 561, 765, 1176, 2130
MaeII	ACGT	1 cut(s)	337
MaeIII	GTNAC	4 cut(s)	816, 1406, 1526, 2132
MalI	GATC	9 cut(s)	42, 177, 239, 911, 933, 1335, 1371, 1730, 1958
MboI	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
MfeI	CAATTG	2 cut(s)	170, 1701
MhlI	GDGCHC	1 cut(s)	1786
MlsI	TGGCCA	1 cut(s)	1508
MluCI	AATT	9 cut(s)	170, 322, 572, 835, 1050, 1271, 1312, 1532, 1701
MluNI	TGGCCA	1 cut(s)	1508
Mly113I	GGCGCC	1 cut(s)	793
MlyI	GAGTC	4 cut(s)	1077, 1222, 2014, 2134
MmeI	TCCRAC	2 cut(s)	235, 785
Mox20I	TGGCCA	1 cut(s)	1508
Mph1103I	ATGCAT	1 cut(s)	1092
MroXI	GAANNNNTTC	1 cut(s)	968
MscI	TGGCCA	1 cut(s)	1508
MseI	TTAA	9 cut(s)	90, 516, 647, 849, 977, 1031, 1113, 1274, 1832
MslI	CAYNNNNRTG	3 cut(s)	314, 382, 1160
Msp20I	TGGCCA	1 cut(s)	1508
MspA1I	CMGCKG	1 cut(s)	398
MspCI	CTTAAG	1 cut(s)	976
MspI	CCGG	4 cut(s)	1379, 1524, 1826, 2090
MspR9I	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 2091, 2100
MunI	CAATTG	2 cut(s)	170, 1701
MvaI	CCWGG	4 cut(s)	822, 1244, 1292, 2100
MvnI	CGCG	3 cut(s)	914, 1640, 2031
MwoI	GCNNNNNNNGC	6 cut(s)	374, 502, 801, 1505, 1880, 1889
NarI	GGCGCC	1 cut(s)	793
NciI	CCSGG	2 cut(s)	1524, 2091
NcoI	CCATGG	1 cut(s)	812
NdeI	CATATG	1 cut(s)	829
NdeII	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
NheI	GCTAGC	1 cut(s)	1175
NlaIV	GGNNCC	8 cut(s)	114, 351, 794, 1698, 1743, 1824, 2095, 2096
NmeAIII	GCCGAG	2 cut(s)	313, 1949
NmuCI	GTSAC	1 cut(s)	816
NruI	TCGCGA	1 cut(s)	1640
NsbI	TGCGCA	1 cut(s)	1882
NsiI	ATGCAT	1 cut(s)	1092
NspI	RCATGY	3 cut(s)	859, 1680, 1943
PaeR7I	CTCGAG	2 cut(s)	1257, 1589
PceI	AGGCCT	1 cut(s)	1250
PciSI	GCTCTTC	2 cut(s)	503, 2110
PdmI	GAANNNNTTC	1 cut(s)	968
PfeI	GAWTC	5 cut(s)	30, 99, 1280, 1467, 1578
PflMI	CCANNNNNTGG	2 cut(s)	1064, 1706
PkrI	GCNGC	5 cut(s)	507, 1297, 1398, 1447, 1807
PleI	GAGTC	4 cut(s)	1076, 1222, 2013, 2133
PluTI	GGCGCC	1 cut(s)	796
PpsI	GAGTC	4 cut(s)	1076, 1222, 2013, 2133
PpuMI	RGGWCCY	1 cut(s)	2094
PshAI	GACNNNNGTC	1 cut(s)	348
Psp5II	RGGWCCY	1 cut(s)	2094
Psp6I	CCWGG	4 cut(s)	820, 1242, 1290, 2098
PspEI	GGTNACC	2 cut(s)	816, 1526
PspFI	CCCAGC	2 cut(s)	464, 1706
PspGI	CCWGG	4 cut(s)	820, 1242, 1290, 2098
PspN4I	GGNNCC	8 cut(s)	114, 351, 794, 1698, 1743, 1824, 2095, 2096
PspPI	GGNCC	3 cut(s)	350, 372, 2094
PspPPI	RGGWCCY	1 cut(s)	2094
PspXI	VCTCGAGB	1 cut(s)	1589
PvuII	CAGCTG	1 cut(s)	398
RruI	TCGCGA	1 cut(s)	1640
RsaI	GTAC	4 cut(s)	184, 308, 1667, 1814
RsaNI	GTAC	4 cut(s)	183, 307, 1666, 1813
RseI	CAYNNNNRTG	3 cut(s)	314, 382, 1160
SapI	GCTCTTC	2 cut(s)	503, 2110
SaqAI	TTAA	9 cut(s)	90, 516, 647, 849, 977, 1031, 1113, 1274, 1832
SatI	GCNGC	5 cut(s)	506, 1296, 1397, 1446, 1806
Sau3AI	GATC	9 cut(s)	40, 175, 237, 909, 931, 1333, 1369, 1728, 1956
Sau96I	GGNCC	3 cut(s)	350, 372, 2094
ScaI	AGTACT	1 cut(s)	184
SchI	GAGTC	4 cut(s)	1077, 1222, 2014, 2134
ScrFI	CCNGG	6 cut(s)	822, 1244, 1292, 1524, 2091, 2100
SduI	GDGCHC	1 cut(s)	1786
SexAI	ACCWGGT	1 cut(s)	820
SfaNI	GCATC	6 cut(s)	792, 953, 1198, 1312, 1792, 1879
SfoI	GGCGCC	1 cut(s)	794
Sfr274I	CTCGAG	2 cut(s)	1257, 1589
SinI	GGWCC	3 cut(s)	350, 372, 2094
SlaI	CTCGAG	2 cut(s)	1257, 1589
SmiMI	CAYNNNNRTG	3 cut(s)	314, 382, 1160
SmlI	CTYRAG	3 cut(s)	976, 1257, 1589
SmoI	CTYRAG	3 cut(s)	976, 1257, 1589
SpeI	ACTAGT	2 cut(s)	560, 764
Sse9I	AATT	9 cut(s)	170, 322, 572, 835, 1050, 1271, 1312, 1532, 1701
SseBI	AGGCCT	1 cut(s)	1250
SsiI	CCGC	5 cut(s)	25, 375, 914, 1421, 2070
SspDI	GGCGCC	1 cut(s)	792
SspI	AATATT	2 cut(s)	423, 1899
SspMI	CTAG	5 cut(s)	365, 561, 765, 1176, 2130
StuI	AGGCCT	1 cut(s)	1250
StyD4I	CCNGG	6 cut(s)	820, 1242, 1290, 1522, 2089, 2098
StyI	CCWWGG	3 cut(s)	788, 812, 1999
TaiI	ACGT	1 cut(s)	340
TaqI	TCGA	5 cut(s)	178, 221, 1258, 1301, 1590
TasI	AATT	9 cut(s)	170, 322, 572, 835, 1050, 1271, 1312, 1532, 1701
TatI	WGTACW	2 cut(s)	182, 306
TfiI	GAWTC	5 cut(s)	30, 99, 1280, 1467, 1578
Tru1I	TTAA	9 cut(s)	90, 516, 647, 849, 977, 1031, 1113, 1274, 1832
Tru9I	TTAA	9 cut(s)	90, 516, 647, 849, 977, 1031, 1113, 1274, 1832
TscAI	CASTG	3 cut(s)	210, 688, 943
TseFI	GTSAC	1 cut(s)	816
TseI	GCWGC	5 cut(s)	505, 1295, 1396, 1445, 1805
Tsp45I	GTSAC	1 cut(s)	816
TspDTI	ATGAA	8 cut(s)	147, 304, 490, 573, 846, 883, 1284, 1492
TspGWI	ACGGA	1 cut(s)	1865
TspRI	CASTG	3 cut(s)	210, 688, 943
Van91I	CCANNNNNTGG	2 cut(s)	1064, 1706
Vha464I	CTTAAG	1 cut(s)	976
VpaK11BI	GGWCC	3 cut(s)	350, 372, 2094
XapI	RAATTY	1 cut(s)	1050
XceI	RCATGY	3 cut(s)	859, 1680, 1943
XhoI	CTCGAG	2 cut(s)	1257, 1589
XmiI	GTMKAC	1 cut(s)	481
XmnI	GAANNNNTTC	1 cut(s)	968
XspI	CTAG	5 cut(s)	365, 561, 765, 1176, 2130
ZrmI	AGTACT	1 cut(s)	184
Zsp2I	ATGCAT	1 cut(s)	1092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.