Rorug07G0009500

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001955
Physical Location & Seq
Forward (+)
785326 .. 788722
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0009500.1

Sequence Viewer

Length: 1284 bp
ATGGCGTCTCTTAGCTTGACTCAGATCAGAGGCAACAACCCAGTTGCCGAGTTTGCCAGAACTCGTTCTCCTCAGTTGGGTTTCCCTCTTCGGTCTAGCAGAGTTGGGTTTCGGGTGTTTGCGTCAGAGAGCAGCAAGGGCCCAGAGCCTGATCTGAGCGTTGATGTAAATGGGTTGCACATGCCCAACCCTTTCGTTATCGGGTCGGGTCCACCCGGTACTAACTACACCGTCATGAAAAGGGCCTTTGATGAAGGCTGGGGTGCTGTGATTGCCAAAACTGTGTCGCTAGACGCAGCAAAAGTGAAGAATGTCACTCCACGATATGCAAAGCTACGTGCTGATGCAAATGGCTCAGCCAAAGGGCAGATTATTGGGTGGGAGAATATAGAACTTATAAGTGATCGACCTCTTGAAACAATGTTGAAAGAATTCAAGCAATTGAAGGAAGAGTATCCGGACAGGATTCTGATTGCTTCAATCATGGAGGAATATAACAAAGCTGGCTGGGAGGAACTTATTGATCGTGTGGAGCAAACTGGAGTTGATGCCTTTGAAATCAACTTCTCCTGTCCTCATGGTATGCCGGAGCGCAAGATGGGTGCTGCTGTTGGGCAAGACTGTGTACTGCTGGAAGAAGTCTGTGGATGGATCAATGCCAAAGCTACAATTCCGGTTTGGGCAAAGATGACTCCTAACGTCACTGACATTACACAGCCAGCTAGAGTGGCTCTTAGCTCAGGATGTGAGGGAGTATCTGCTATTAACACGATCATGAGTGTAATGGGAATCAATCTGACTACCTTACGGCCTGAGCCTTGTGTGGAGGGATACTCGACTCCTGGGGGCTATTCTTCCAAGGCAGTTCATCCTATTGCACTTGCAAAAGTGATGAGTATCGCAAAAATGATGAAATCAGAATTTGCTAATAAAGATCTTTCACTTTCTGGGATCGGTGGTGTTGAGACCGGTGGTGATGCTGCTGAATTTATCCTTCTGGGAGCAAATTCTGTTCAGGTCTGTACTGGGGTTATGATGCATGGGTACGGCGTTGTGAAGAAACTCTGTTCTGAGCTAAAGGATTTCATGAGACAGCACAATTTCTCATCTATAGAAGATTTCAGAGGGGCTTCCCTCGAGTATTTTACAACGCATACTGATTTAGTACGCAGACAGCAAGAAGCCATCCTACAAAGAAAAGCTGTGAAGAGAGGGTTGCAGTCCGACAAAGATTGGACAGGAGATGGTTTCGTTAAAGAGACCGAGAGCATGGTCTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

427

Amino Acids

46.55

Weight (kDa)

6.16

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHO_dh PF01180 52 - 363 8.4e-35 Dihydroorotate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 398
AccIII TCCGGA 1 cut(s) 457
AclWI GGATC 2 cut(s) 659, 959
AcsI RAATTY 4 cut(s) 431, 920, 986, 1006
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 220, 627, 1024, 1046, 1167
AfiI CCNNNNNNNGG 1 cut(s) 77
AgeI ACCGGT 1 cut(s) 968
AgsI TTSAA 6 cut(s) 416, 427, 436, 445, 480, 557
AjnI CCWGG 1 cut(s) 841
AloI GAACNNNNNNTCC 2 cut(s) 52, 84
AluBI AGCT 8 cut(s) 15, 334, 503, 665, 722, 738, 1075, 1202
AluI AGCT 8 cut(s) 15, 334, 503, 665, 722, 738, 1075, 1202
Alw26I GTCTC 5 cut(s) 12, 959, 1084, 1253, 1279
AlwI GGATC 2 cut(s) 659, 959
AlwNI CAGNNNCTG 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 1136
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 3 cut(s) 139, 243, 809
ApaI GGGCCC 1 cut(s) 143
ApeKI GCWGC 4 cut(s) 132, 296, 605, 980
ApoI RAATTY 4 cut(s) 431, 920, 986, 1006
AsiGI ACCGGT 1 cut(s) 968
Asp700I GAANNNNTTC 2 cut(s) 64, 431
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 4 cut(s) 139, 140, 209, 243
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 986
AvaI CYCGRG 1 cut(s) 1136
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 2 cut(s) 1173, 1205
BbvI GCAGC 4 cut(s) 144, 308, 592, 967
BccI CCATC 4 cut(s) 592, 642, 1193, 1238
BceAI ACGGC 2 cut(s) 824, 1063
BciT130I CCWGG 1 cut(s) 843
BciVI GTATCC 2 cut(s) 465, 824
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 5 cut(s) 12, 959, 1084, 1253, 1279
BfaI CTAG 3 cut(s) 96, 290, 723
BfmI CTRYAG 1 cut(s) 1110
BfuI GTATCC 2 cut(s) 465, 824
BglII AGATCT 1 cut(s) 934
BisI GCNGC 4 cut(s) 133, 297, 606, 981
BlpI GCTNAGC 1 cut(s) 355
BlsI GCNGC 4 cut(s) 134, 298, 607, 982
Bme1390I CCNGG 2 cut(s) 216, 843
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 1136
BmgT120I GGNCC 4 cut(s) 139, 140, 209, 243
BmiI GGNNCC 2 cut(s) 141, 210
BmrFI CCNGG 2 cut(s) 216, 843
BmrI ACTGGG 2 cut(s) 35, 1035
BmsI GCATC 4 cut(s) 334, 538, 967, 1026
BmuI ACTGGG 2 cut(s) 35, 1035
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 2 cut(s) 739, 813
Bpu1102I GCTNAGC 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 216
BsaAI YACGTR 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 896
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 959, 1253, 1279
BsaJI CCNNGG 2 cut(s) 842, 858
BsaWI WCCGGW 3 cut(s) 457, 673, 968
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 3 cut(s) 41, 544, 1030
Bse8I GATNNNNATC 1 cut(s) 896
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 2 cut(s) 842, 858
BseGI GGATG 4 cut(s) 653, 749, 868, 1185
BseJI GATNNNNATC 1 cut(s) 896
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 7 cut(s) 35, 86, 146, 369, 753, 804, 1062
BseNI ACTGG 3 cut(s) 41, 544, 1030
BseRI GAGGAG 1 cut(s) 60
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 4 cut(s) 144, 308, 592, 967
BseYI CCCAGC 2 cut(s) 258, 507
BshFI GGCC 3 cut(s) 141, 245, 811
BshTI ACCGGT 1 cut(s) 968
BsiHKCI CYCGRG 1 cut(s) 1136
BsiSI CCGG 5 cut(s) 216, 458, 587, 674, 969
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 5 cut(s) 12, 959, 1084, 1253, 1279
BsmBI CGTCTC 1 cut(s) 12
BsnI GGCC 3 cut(s) 141, 245, 811
Bso31I GGTCTC 3 cut(s) 959, 1253, 1279
BsoBI CYCGRG 1 cut(s) 1136
Bsp120I GGGCCC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 143
Bsp13I TCCGGA 1 cut(s) 457
Bsp143I GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
Bsp1720I GCTNAGC 1 cut(s) 355
BspANI GGCC 3 cut(s) 141, 245, 811
BspCNI CTCAG 7 cut(s) 34, 85, 147, 368, 752, 805, 1063
BspEI TCCGGA 1 cut(s) 457
BspHI TCATGA 3 cut(s) 234, 774, 1086
BspLI GGNNCC 2 cut(s) 141, 210
BspPI GGATC 2 cut(s) 659, 959
BspTNI GGTCTC 3 cut(s) 959, 1253, 1279
BsrFI RCCGGY 1 cut(s) 968
BsrI ACTGG 3 cut(s) 41, 544, 1030
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 2 cut(s) 842, 858
BssMI GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 858
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 232, 283, 623
Bst6I CTCTTC 3 cut(s) 93, 444, 1202
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 505, 720
BstDEI CTNAG 9 cut(s) 11, 21, 72, 155, 355, 734, 739, 813, 1071
BstF5I GGATG 4 cut(s) 653, 749, 868, 1185
BstHHI GCGC 1 cut(s) 594
BstKTI GATC 8 cut(s) 27, 154, 406, 526, 654, 774, 937, 954
BstMAI GTCTC 5 cut(s) 12, 959, 1084, 1253, 1279
BstMBI GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
BstMWI GCNNNNNNNGC 4 cut(s) 53, 138, 272, 728
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 2 cut(s) 214, 841
BstSFI CTRYAG 1 cut(s) 1110
BstSLI GKGCMC 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 144, 308, 592, 967
BstX2I RGATCY 1 cut(s) 934
BstYI RGATCY 1 cut(s) 934
BsuI GTATCC 2 cut(s) 465, 824
BsuRI GGCC 3 cut(s) 141, 245, 811
BtsCI GGATG 4 cut(s) 653, 749, 868, 1185
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 2 cut(s) 505, 720
CaiI CAGNNNCTG 1 cut(s) 149
CciI TCATGA 3 cut(s) 234, 774, 1086
CfoI GCGC 1 cut(s) 594
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 4 cut(s) 139, 140, 209, 243
CseI GACGC 2 cut(s) 111, 302
Csp6I GTAC 5 cut(s) 219, 626, 1023, 1045, 1166
CspAI ACCGGT 1 cut(s) 968
CviAII CATG 8 cut(s) 181, 235, 484, 578, 775, 1040, 1087, 1270
CviQI GTAC 5 cut(s) 219, 626, 1023, 1045, 1166
DdeI CTNAG 9 cut(s) 11, 21, 72, 155, 355, 734, 739, 813, 1071
DpnI GATC 8 cut(s) 26, 153, 405, 525, 653, 773, 936, 953
DpnII GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
Eam1104I CTCTTC 3 cut(s) 93, 444, 1202
EarI CTCTTC 3 cut(s) 93, 444, 1202
Eco130I CCWWGG 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 3 cut(s) 959, 1253, 1279
Eco47I GGWCC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 1136
EcoO109I RGGNCCY 2 cut(s) 139, 243
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 1 cut(s) 1041
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 858
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 8 cut(s) 184, 238, 487, 581, 778, 1043, 1090, 1273
FatI CATG 8 cut(s) 180, 234, 483, 577, 774, 1039, 1086, 1269
Fnu4HI GCNGC 4 cut(s) 133, 297, 606, 981
FokI GGATG 4 cut(s) 660, 756, 855, 1172
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 4 cut(s) 133, 297, 606, 981
FspBI CTAG 3 cut(s) 96, 290, 723
GlaI GCGC 1 cut(s) 593
GluI GCNGC 4 cut(s) 133, 297, 606, 981
GsaI CCCAGC 2 cut(s) 262, 511
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 3 cut(s) 141, 245, 811
HapII CCGG 5 cut(s) 216, 458, 587, 674, 969
HgaI GACGC 2 cut(s) 111, 302
HhaI GCGC 1 cut(s) 594
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 184, 238, 487, 581, 778, 1043, 1090, 1273
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HinfI GANTC 5 cut(s) 19, 466, 691, 789, 838
HpaII CCGG 5 cut(s) 216, 458, 587, 674, 969
HphI GGTGA 1 cut(s) 986
Hpy166II GTNNAC 2 cut(s) 212, 626
Hpy188III TCNNGA 6 cut(s) 235, 413, 458, 741, 775, 1087
Hpy8I GTNNAC 2 cut(s) 212, 626
HpyAV CCTTC 3 cut(s) 248, 439, 1004
HpyCH4III ACNGT 3 cut(s) 232, 283, 623
HpyCH4IV ACGT 2 cut(s) 337, 699
HpyCH4V TGCA 7 cut(s) 178, 329, 347, 878, 884, 1039, 1219
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 138, 272, 728
HpyF3I CTNAG 9 cut(s) 11, 21, 72, 155, 355, 734, 739, 813, 1071
HpySE526I ACGT 2 cut(s) 337, 699
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 184, 238, 487, 581, 778, 1043, 1090, 1273
HspAI GCGC 1 cut(s) 592
Kpn2I TCCGGA 1 cut(s) 457
Kzo9I GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
LmnI GCTCC 3 cut(s) 532, 589, 1001
Lsp1109I GCAGC 4 cut(s) 144, 308, 592, 967
LweI GCATC 4 cut(s) 334, 538, 967, 1026
MaeI CTAG 3 cut(s) 96, 290, 723
MaeII ACGT 2 cut(s) 337, 699
MaeIII GTNAC 2 cut(s) 313, 700
MalI GATC 8 cut(s) 26, 153, 405, 525, 653, 773, 936, 953
MboI GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
MboII GAAGA 8 cut(s) 80, 319, 461, 647, 846, 1069, 1127, 1219
MfeI CAATTG 1 cut(s) 440
MflI RGATCY 1 cut(s) 934
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 8 cut(s) 431, 440, 669, 920, 986, 1006, 1099, 1279
MlyI GAGTC 3 cut(s) 13, 685, 832
MmeI TCCRAC 1 cut(s) 1248
Mph1103I ATGCAT 1 cut(s) 1041
MroI TCCGGA 1 cut(s) 457
MroXI GAANNNNTTC 2 cut(s) 64, 431
MseI TTAA 3 cut(s) 765, 1254, 1282
MslI CAYNNNNRTG 2 cut(s) 233, 773
MspI CCGG 5 cut(s) 216, 458, 587, 674, 969
MspR9I CCNGG 2 cut(s) 216, 843
MunI CAATTG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 4 cut(s) 53, 138, 272, 728
NciI CCSGG 1 cut(s) 216
NdeII GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
NlaIII CATG 8 cut(s) 184, 238, 487, 581, 778, 1043, 1090, 1273
NlaIV GGNNCC 2 cut(s) 141, 210
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 2 cut(s) 313, 700
NsiI ATGCAT 1 cut(s) 1041
NspI RCATGY 1 cut(s) 184
PaeR7I CTCGAG 1 cut(s) 1136
PagI TCATGA 3 cut(s) 234, 774, 1086
PdmI GAANNNNTTC 2 cut(s) 64, 431
PfeI GAWTC 2 cut(s) 466, 789
PinAI ACCGGT 1 cut(s) 968
PkrI GCNGC 4 cut(s) 134, 298, 607, 982
PleI GAGTC 3 cut(s) 13, 685, 832
PpsI GAGTC 3 cut(s) 13, 685, 832
Ppu21I YACGTR 1 cut(s) 338
PsiI TTATAA 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 841
PspFI CCCAGC 2 cut(s) 258, 507
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 2 cut(s) 141, 210
PspOMI GGGCCC 1 cut(s) 139
PspPI GGNCC 4 cut(s) 139, 140, 209, 243
PspXI VCTCGAGB 1 cut(s) 1136
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 1 cut(s) 934
RsaI GTAC 5 cut(s) 220, 627, 1024, 1046, 1167
RsaNI GTAC 5 cut(s) 219, 626, 1023, 1045, 1166
RseI CAYNNNNRTG 2 cut(s) 233, 773
SaqAI TTAA 3 cut(s) 765, 1254, 1282
SatI GCNGC 4 cut(s) 133, 297, 606, 981
Sau3AI GATC 8 cut(s) 24, 151, 403, 523, 651, 771, 934, 951
Sau96I GGNCC 4 cut(s) 139, 140, 209, 243
SchI GAGTC 3 cut(s) 13, 685, 832
ScrFI CCNGG 2 cut(s) 216, 843
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 4 cut(s) 334, 538, 967, 1026
SfcI CTRYAG 1 cut(s) 1110
Sfr274I CTCGAG 1 cut(s) 1136
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 1136
SmiMI CAYNNNNRTG 2 cut(s) 233, 773
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
Sse9I AATT 8 cut(s) 431, 440, 669, 920, 986, 1006, 1099, 1279
SspMI CTAG 3 cut(s) 96, 290, 723
StyD4I CCNGG 2 cut(s) 214, 841
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 3 cut(s) 232, 283, 623
TaiI ACGT 2 cut(s) 340, 702
TaqI TCGA 3 cut(s) 406, 836, 1137
TaqII GACCGA 2 cut(s) 81, 1277
TasI AATT 8 cut(s) 431, 440, 669, 920, 986, 1006, 1099, 1279
TatI WGTACW 2 cut(s) 625, 1022
TfiI GAWTC 2 cut(s) 466, 789
Tru1I TTAA 3 cut(s) 765, 1254, 1282
Tru9I TTAA 3 cut(s) 765, 1254, 1282
TscAI CASTG 1 cut(s) 709
TseFI GTSAC 2 cut(s) 313, 700
TseI GCWGC 4 cut(s) 132, 296, 605, 980
Tsp45I GTSAC 2 cut(s) 313, 700
TspDTI ATGAA 5 cut(s) 251, 267, 857, 926, 1075
TspRI CASTG 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 4 cut(s) 431, 920, 986, 1006
XceI RCATGY 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 1136
XmnI GAANNNNTTC 2 cut(s) 64, 431
XspI CTAG 3 cut(s) 96, 290, 723
Zsp2I ATGCAT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.