Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0013521

Physical Location & Seq

Genomic Coordinates Forward (+)

1227623 .. 1229167

Gene Structure 1545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1008400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGTACTTTACCCAATTTGTATTGAATTATGCATGGGCAGCAATTCCAAACGGCAATTGCAACAACATATGCATGGGCGGCAATTCTCTGTTAAACCTTCTTCTCTGCGACAAAGGCCATCTGACCAAACCCTCAAATCCCGCCACCGCCAAAGCCGTTACCACCACCATTCTCAACCACCTCGACTCCGGCCGTCTCCGAAAAGCCGTCTCAATCCTCTTTTATGCCCCATTCCCCTTCCATTTTTCTCTATACGCTCGACTCTTCCAGCTTTGTTCCTCCACCGGCAGCCGTGCCATTGTCGAGGCCCGCAAGGTCGAGTCCCATTTGTTTACATTCTCCCCCACCCCGCCGGTGTTTCTAGTGAACCGTGCCATCGAGGCCTTTGCAAAATGCGGGTCTTTGGGGGATGCGAGGGAGCTGTTCGAGGAAATGCCTCAGAGAGATGGGGGATCTTGGAATGCTTTGATAACGGCGTGCTCACAAAGTGGGTCTCCTGAGGAAGCCTTTCGCTTGTTTGTGAAAATGAGTAGGTCAGGTTTTTTGCCGAATGAGATTACGTTTGCTAGTGTTCTTGGCTCCTGTGCTGCTGTTTTGGCGCTTTCGCTTTCGAGGCAAGTTCATGGACTAATTGTCAAGTATGGTTTTGGCGGGAATGTGGTTATAGGGAGTTCACTTGTTGACATATACGGGAAATGCGTGATTATGGATGATGCCTGTAAAATATTTGACGAGATTAAGAACCCGAATGACATTTCATGGAACATAATCGTGAGGCGGTATCTGGAGATGGGTGAAGGAGAGGAGGCAATAGTTATGTTTTTCCAGATGTTTAAGGCAGCTGTAAGGCCACTTAAGTTTACCTTTTCTAATGCTCTTGTTGCTTGTTCGAGTATTTCTGCACTTGATGAGGGGATACAGATTCATGGAGTTGCAATTAAAATGGGGTTCGATGACGATGAGATTGTTTCAAGCTCCCTTATCGATATGTATGCTAAGTGTGGGGATTTAGAGAGTGCCAGTGCAATTTTTGACCAGCCCAATTCAAAAGATGTGATTTCTTGGACTTCAATTGTTTCTGGGTATGCAATGAGTGGACAGACATGGAAGGCCAGAGAGCTCTTCGATGAGATGCCCGAACGCAATGTAATATCTTGGAATGCAATGCTGGCCGGGTATACTCGCTACTCCCAGTGGGAAGAGGCAGTGGATTTTCTATTTTTGATGCGGAACACAACCAATAATATTGATCAGGTCACTCTTGGGTTGATCCTCAAGGTGTGTGCTGGCCTTTTGGATGTGGAATTGGGGAAACAGGTGCATGGATTCATATACCGGCACGGTTTCTCTTCCAGTATGTTTGTTGGCAATGGCCTTCTTCACATGTATAGTAAATGTGGGAAAATGAGAAGTGCCAAAACAGTCTGGTTTCACCAAATTAGTCACTGGCGGGATAGAGTTTCTTCAAATGCTTTGTTGAGTAGCTATGCTCGTCGTGGCCGGAGTGAACTAGCTATGACAATTTTCTGCGGGATGCAATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

515

Amino Acids

57.05

Weight (kDa)

7.52

Isoelectric Point (pI)

44.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	152 - 196	1.4e-10	PPR repeat family
PPR	PF01535	152 - 181	1.2e-07	PPR repeat
PPR	PF01535	353 - 383	9.9e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26540
fragaria_vesca	FvH4_4g16500 FvH4_4g16500 FvH4_4g16500 FvH4_4g16500
malus_domestica	MD10G1008400.v1.1
prunus_persica	Prupe.8G008000_v2.0.a1
pyrus_communis	pycom10g00510
rosa_chinensis	RchiOBHm_Chr4g0415151
rosa_laevigata	RLG00000008092
rosa_multiflora	Rmu_sc0003748.1_g000028
rosa_roxburghii	Rroxscaffold_5G00358960
rosa_rugosa	Rorug04G0132500
rosa_samantha	Rh4AG192500 Rh4BG190700 Rh4CG203400 Rh4DG190400
rosa_wichuraiana	Rw4G016370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1178
AclWI	GGATC	2 cut(s)	460, 1264
AcoI	YGGCCR	3 cut(s)	190, 1170, 1498
AdeI	CACNNNGTG	1 cut(s)	488
AfaI	GTAC	1 cut(s)	5
AflII	CTTAAG	1 cut(s)	854
AflIII	ACRYGT	1 cut(s)	1383
AgsI	TTSAA	5 cut(s)	25, 972, 1047, 1071, 1467
AhdI	GACNNNNNGTC	1 cut(s)	632
AluBI	AGCT	7 cut(s)	271, 421, 842, 975, 1120, 1485, 1514
AluI	AGCT	7 cut(s)	271, 421, 842, 975, 1120, 1485, 1514
Alw21I	GWGCWC	2 cut(s)	482, 1122
Alw26I	GTCTC	3 cut(s)	200, 214, 498
AlwI	GGATC	2 cut(s)	460, 1264
ApeKI	GCWGC	4 cut(s)	38, 288, 587, 839
ArsI	GACNNNNNNTTYG	2 cut(s)	383, 415
Asp700I	GAANNNNTTC	1 cut(s)	507
AspLEI	GCGC	1 cut(s)	601
AspS9I	GGNCC	1 cut(s)	307
AsuC2I	CCSGG	1 cut(s)	1174
AsuHPI	GGTGA	2 cut(s)	806, 1424
AxyI	CCTNAGG	1 cut(s)	498
BanII	GRGCYC	1 cut(s)	1122
Bbv12I	GWGCWC	2 cut(s)	482, 1122
BbvI	GCAGC	4 cut(s)	50, 300, 574, 851
BccI	CCATC	4 cut(s)	126, 383, 440, 784
BceAI	ACGGC	6 cut(s)	67, 140, 177, 191, 276, 489
BcgI	CGANNNNNNTGC	4 cut(s)	368, 402, 974, 1008
BciVI	GTATCC	1 cut(s)	909
BclI	TGATCA	1 cut(s)	1249
BcnI	CCSGG	1 cut(s)	1174
BcoDI	GTCTC	3 cut(s)	200, 214, 498
BfaI	CTAG	3 cut(s)	362, 567, 1511
BfoI	RGCGCY	1 cut(s)	602
BfrI	CTTAAG	1 cut(s)	854
BfuI	GTATCC	1 cut(s)	909
BglI	GCCNNNNNGGC	1 cut(s)	380
BisI	GCNGC	5 cut(s)	39, 79, 289, 588, 840
BlsI	GCNGC	5 cut(s)	40, 80, 290, 589, 841
Bme1390I	CCNGG	1 cut(s)	1174
BmeRI	GACNNNNNGTC	1 cut(s)	632
BmgT120I	GGNCC	1 cut(s)	307
BmiI	GGNNCC	1 cut(s)	580
BmrFI	CCNGG	1 cut(s)	1174
BmrI	ACTGGG	1 cut(s)	1186
BmsI	GCATC	5 cut(s)	400, 703, 1122, 1215, 1524
BmuI	ACTGGG	1 cut(s)	1186
BpmI	CTGGAG	1 cut(s)	806
BpuEI	CTTGAG	1 cut(s)	1259
BpuMI	CCSGG	1 cut(s)	1174
Bsa29I	ATCGAT	1 cut(s)	984
BsaI	GGTCTC	1 cut(s)	498
BsaXI	ACNNNNNCTCC	2 cut(s)	170, 200
Bse118I	RCCGGY	3 cut(s)	284, 352, 1335
Bse1I	ACTGG	4 cut(s)	1020, 1192, 1353, 1451
Bse21I	CCTNAGG	1 cut(s)	498
Bse3DI	GCAATG	5 cut(s)	1095, 1150, 1170, 1375, 1544
BseCI	ATCGAT	1 cut(s)	984
BseGI	GGATG	4 cut(s)	415, 715, 1303, 1539
BseMI	GCAATG	5 cut(s)	1095, 1150, 1170, 1375, 1544
BseMII	CTCAG	2 cut(s)	452, 489
BseNI	ACTGG	4 cut(s)	1020, 1192, 1353, 1451
BseRI	GAGGAG	1 cut(s)	818
BseX3I	CGGCCG	1 cut(s)	190
BseXI	GCAGC	4 cut(s)	50, 300, 574, 851
BsgI	GTGCAG	1 cut(s)	885
Bsh1285I	CGRYCG	1 cut(s)	193
BshVI	ATCGAT	1 cut(s)	984
BsiEI	CGRYCG	1 cut(s)	193
BsiHKAI	GWGCWC	2 cut(s)	482, 1122
BsiSI	CCGG	6 cut(s)	189, 285, 353, 1173, 1336, 1501
BslFI	GGGAC	1 cut(s)	307
BsmAI	GTCTC	3 cut(s)	200, 214, 498
BsmBI	CGTCTC	2 cut(s)	200, 214
BsmFI	GGGAC	1 cut(s)	307
BsmI	GAATGC	2 cut(s)	466, 1165
Bso31I	GGTCTC	1 cut(s)	498
Bsp1286I	GDGCHC	2 cut(s)	482, 1122
Bsp143I	GATC	3 cut(s)	452, 1249, 1269
BspCNI	CTCAG	2 cut(s)	451, 490
BspDI	ATCGAT	1 cut(s)	984
BspLI	GGNNCC	1 cut(s)	580
BspPI	GGATC	2 cut(s)	460, 1264
BspQI	GCTCTTC	1 cut(s)	1127
BspTI	CTTAAG	1 cut(s)	854
BspTNI	GGTCTC	1 cut(s)	498
BsrDI	GCAATG	5 cut(s)	1095, 1150, 1170, 1375, 1544
BsrFI	RCCGGY	3 cut(s)	284, 352, 1335
BsrI	ACTGG	4 cut(s)	1020, 1192, 1353, 1451
BssAI	RCCGGY	3 cut(s)	284, 352, 1335
BssMI	GATC	3 cut(s)	452, 1249, 1269
BssNAI	GTATAC	1 cut(s)	1179
Bst1107I	GTATAC	1 cut(s)	1179
Bst4CI	ACNGT	3 cut(s)	371, 1343, 1423
Bst6I	CTCTTC	4 cut(s)	269, 1127, 1194, 1354
BstAFI	CTTAAG	1 cut(s)	854
BstC8I	GCNNGC	4 cut(s)	310, 478, 1170, 1288
BstDEI	CTNAG	3 cut(s)	438, 498, 996
BstF5I	GGATG	4 cut(s)	415, 715, 1303, 1539
BstH2I	RGCGCY	1 cut(s)	602
BstHHI	GCGC	1 cut(s)	601
BstKTI	GATC	3 cut(s)	455, 1252, 1272
BstMAI	GTCTC	3 cut(s)	200, 214, 498
BstMBI	GATC	3 cut(s)	452, 1249, 1269
BstMCI	CGRYCG	1 cut(s)	193
BstMWI	GCNNNNNNNGC	8 cut(s)	38, 78, 114, 380, 596, 613, 881, 1169
BstNSI	RCATGY	1 cut(s)	1387
BstSCI	CCNGG	1 cut(s)	1172
BstV1I	GCAGC	4 cut(s)	50, 300, 574, 851
BstX2I	RGATCY	1 cut(s)	452
BstYI	RGATCY	1 cut(s)	452
BstZ17I	GTATAC	1 cut(s)	1179
BstZI	CGGCCG	1 cut(s)	190
Bsu15I	ATCGAT	1 cut(s)	984
Bsu36I	CCTNAGG	1 cut(s)	498
BsuI	GTATCC	1 cut(s)	909
BsuTUI	ATCGAT	1 cut(s)	984
BtsCI	GGATG	4 cut(s)	415, 715, 1303, 1539
BtsI	GCAGTG	1 cut(s)	1212
BtsIMutI	CAGTG	4 cut(s)	1027, 1199, 1212, 1444
Cac8I	GCNNGC	4 cut(s)	310, 478, 1170, 1288
CfoI	GCGC	1 cut(s)	601
Cfr10I	RCCGGY	3 cut(s)	284, 352, 1335
Cfr13I	GGNCC	1 cut(s)	307
ClaI	ATCGAT	1 cut(s)	984
Csp6I	GTAC	1 cut(s)	4
CviAII	CATG	8 cut(s)	33, 73, 623, 759, 926, 1104, 1322, 1384
CviQI	GTAC	1 cut(s)	4
DdeI	CTNAG	3 cut(s)	438, 498, 996
DpnI	GATC	3 cut(s)	454, 1251, 1271
DpnII	GATC	3 cut(s)	452, 1249, 1269
DraIII	CACNNNGTG	1 cut(s)	488
DriI	GACNNNNNGTC	1 cut(s)	632
EaeI	YGGCCR	3 cut(s)	190, 1170, 1498
EagI	CGGCCG	1 cut(s)	190
Eam1104I	CTCTTC	4 cut(s)	269, 1127, 1194, 1354
Eam1105I	GACNNNNNGTC	1 cut(s)	632
EarI	CTCTTC	4 cut(s)	269, 1127, 1194, 1354
Ecl136II	GAGCTC	1 cut(s)	1120
EclXI	CGGCCG	1 cut(s)	190
Eco147I	AGGCCT	1 cut(s)	383
Eco24I	GRGCYC	1 cut(s)	1122
Eco31I	GGTCTC	1 cut(s)	498
Eco52I	CGGCCG	1 cut(s)	190
Eco53kI	GAGCTC	1 cut(s)	1120
Eco81I	CCTNAGG	1 cut(s)	498
EcoICRI	GAGCTC	1 cut(s)	1120
EcoT22I	ATGCAT	2 cut(s)	34, 74
EcoT38I	GRGCYC	1 cut(s)	1122
Esp3I	CGTCTC	2 cut(s)	200, 214
FaeI	CATG	8 cut(s)	36, 76, 626, 762, 929, 1107, 1325, 1387
FalI	AAGNNNNNCTT	2 cut(s)	848, 880
FaqI	GGGAC	1 cut(s)	307
FatI	CATG	8 cut(s)	32, 72, 622, 758, 925, 1103, 1321, 1383
FauI	CCCGC	7 cut(s)	148, 317, 357, 389, 644, 1443, 1523
FauNDI	CATATG	1 cut(s)	68
FbaI	TGATCA	1 cut(s)	1249
FblI	GTMKAC	1 cut(s)	1178
Fnu4HI	GCNGC	5 cut(s)	39, 79, 289, 588, 840
FokI	GGATG	3 cut(s)	422, 722, 1310
FriOI	GRGCYC	1 cut(s)	1122
Fsp4HI	GCNGC	5 cut(s)	39, 79, 289, 588, 840
FspBI	CTAG	3 cut(s)	362, 567, 1511
GlaI	GCGC	1 cut(s)	600
GluI	GCNGC	5 cut(s)	39, 79, 289, 588, 840
GsuI	CTGGAG	1 cut(s)	806
HaeII	RGCGCY	1 cut(s)	602
HapII	CCGG	6 cut(s)	189, 285, 353, 1173, 1336, 1501
HhaI	GCGC	1 cut(s)	601
Hin1II	CATG	8 cut(s)	36, 76, 626, 762, 929, 1107, 1325, 1387
Hin6I	GCGC	1 cut(s)	599
HinP1I	GCGC	1 cut(s)	599
HincII	GTYRAC	1 cut(s)	682
HindII	GTYRAC	1 cut(s)	682
HinfI	GANTC	5 cut(s)	185, 261, 320, 922, 1326
HpaII	CCGG	6 cut(s)	189, 285, 353, 1173, 1336, 1501
HphI	GGTGA	2 cut(s)	806, 1424
Hpy166II	GTNNAC	8 cut(s)	333, 367, 674, 682, 861, 1097, 1179, 1508
Hpy188I	TCNGA	3 cut(s)	123, 200, 441
Hpy188III	TCNNGA	4 cut(s)	497, 772, 785, 826
Hpy8I	GTNNAC	8 cut(s)	333, 367, 674, 682, 861, 1097, 1179, 1508
Hpy99I	CGWCG	1 cut(s)	1497
HpyAV	CCTTC	5 cut(s)	107, 247, 791, 1102, 1385
HpyCH4III	ACNGT	3 cut(s)	371, 1343, 1423
HpyCH4IV	ACGT	1 cut(s)	560
HpyF10VI	GCNNNNNNNGC	8 cut(s)	38, 78, 114, 380, 596, 613, 881, 1169
HpyF3I	CTNAG	3 cut(s)	438, 498, 996
HpySE526I	ACGT	1 cut(s)	560
Hsp92II	CATG	8 cut(s)	36, 76, 626, 762, 929, 1107, 1325, 1387
HspAI	GCGC	1 cut(s)	599
Ksp22I	TGATCA	1 cut(s)	1249
Kzo9I	GATC	3 cut(s)	452, 1249, 1269
LguI	GCTCTTC	1 cut(s)	1127
LmnI	GCTCC	3 cut(s)	418, 584, 980
Lsp1109I	GCAGC	4 cut(s)	50, 300, 574, 851
LweI	GCATC	5 cut(s)	400, 703, 1122, 1215, 1524
MaeI	CTAG	3 cut(s)	362, 567, 1511
MaeII	ACGT	1 cut(s)	560
MaeIII	GTNAC	3 cut(s)	157, 1255, 1442
MalI	GATC	3 cut(s)	454, 1251, 1271
MboI	GATC	3 cut(s)	452, 1249, 1269
MboII	GAAGA	7 cut(s)	92, 256, 1114, 1211, 1341, 1370, 1455
MfeI	CAATTG	2 cut(s)	55, 1071
MflI	RGATCY	1 cut(s)	452
MhlI	GDGCHC	2 cut(s)	482, 1122
MlyI	GAGTC	3 cut(s)	179, 255, 329
Mph1103I	ATGCAT	2 cut(s)	34, 74
MroXI	GAANNNNTTC	1 cut(s)	507
MseI	TTAA	5 cut(s)	92, 738, 834, 855, 939
MslI	CAYNNNNRTG	2 cut(s)	71, 770
MspA1I	CMGCKG	1 cut(s)	842
MspCI	CTTAAG	1 cut(s)	854
MspI	CCGG	6 cut(s)	189, 285, 353, 1173, 1336, 1501
MspR9I	CCNGG	1 cut(s)	1174
MunI	CAATTG	2 cut(s)	55, 1071
Mva1269I	GAATGC	2 cut(s)	466, 1165
MwoI	GCNNNNNNNGC	8 cut(s)	38, 78, 114, 380, 596, 613, 881, 1169
NciI	CCSGG	1 cut(s)	1174
NdeI	CATATG	1 cut(s)	68
NdeII	GATC	3 cut(s)	452, 1249, 1269
NlaIII	CATG	8 cut(s)	36, 76, 626, 762, 929, 1107, 1325, 1387
NlaIV	GGNNCC	1 cut(s)	580
NmuCI	GTSAC	2 cut(s)	1255, 1442
NsiI	ATGCAT	2 cut(s)	34, 74
NspI	RCATGY	1 cut(s)	1387
PceI	AGGCCT	1 cut(s)	383
PciI	ACATGT	1 cut(s)	1383
PciSI	GCTCTTC	1 cut(s)	1127
PcsI	WCGNNNNNNNCGW	1 cut(s)	696
PctI	GAATGC	2 cut(s)	466, 1165
PdmI	GAANNNNTTC	1 cut(s)	507
PfeI	GAWTC	2 cut(s)	922, 1326
PkrI	GCNGC	5 cut(s)	40, 80, 290, 589, 841
PleI	GAGTC	3 cut(s)	179, 255, 328
PpsI	GAGTC	3 cut(s)	179, 255, 328
PscI	ACATGT	1 cut(s)	1383
Psp124BI	GAGCTC	1 cut(s)	1122
PspN4I	GGNNCC	1 cut(s)	580
PspPI	GGNCC	1 cut(s)	307
PsuI	RGATCY	1 cut(s)	452
PvuII	CAGCTG	1 cut(s)	842
RsaI	GTAC	1 cut(s)	5
RsaNI	GTAC	1 cut(s)	4
RseI	CAYNNNNRTG	2 cut(s)	71, 770
SacI	GAGCTC	1 cut(s)	1122
SapI	GCTCTTC	1 cut(s)	1127
SaqAI	TTAA	5 cut(s)	92, 738, 834, 855, 939
SatI	GCNGC	5 cut(s)	39, 79, 289, 588, 840
Sau3AI	GATC	3 cut(s)	452, 1249, 1269
Sau96I	GGNCC	1 cut(s)	307
SchI	GAGTC	3 cut(s)	179, 255, 329
ScrFI	CCNGG	1 cut(s)	1174
SduI	GDGCHC	2 cut(s)	482, 1122
SfaNI	GCATC	5 cut(s)	400, 703, 1122, 1215, 1524
SgrAI	CRCCGGYG	1 cut(s)	352
SmiMI	CAYNNNNRTG	2 cut(s)	71, 770
SmlI	CTYRAG	2 cut(s)	854, 1274
SmoI	CTYRAG	2 cut(s)	854, 1274
SseBI	AGGCCT	1 cut(s)	383
SspI	AATATT	2 cut(s)	726, 1246
SspMI	CTAG	3 cut(s)	362, 567, 1511
SstI	GAGCTC	1 cut(s)	1122
StuI	AGGCCT	1 cut(s)	383
StyD4I	CCNGG	1 cut(s)	1172
TaaI	ACNGT	3 cut(s)	371, 1343, 1423
TaiI	ACGT	1 cut(s)	563
TatI	WGTACW	1 cut(s)	3
TauI	GCSGC	1 cut(s)	81
TfiI	GAWTC	2 cut(s)	922, 1326
Tru1I	TTAA	5 cut(s)	92, 738, 834, 855, 939
Tru9I	TTAA	5 cut(s)	92, 738, 834, 855, 939
TscAI	CASTG	4 cut(s)	1027, 1199, 1212, 1451
TseFI	GTSAC	2 cut(s)	1255, 1442
TseI	GCWGC	4 cut(s)	38, 288, 587, 839
Tsp45I	GTSAC	2 cut(s)	1255, 1442
TspDTI	ATGAA	4 cut(s)	611, 747, 914, 1318
TspRI	CASTG	4 cut(s)	1027, 1199, 1212, 1451
Vha464I	CTTAAG	1 cut(s)	854
XceI	RCATGY	1 cut(s)	1387
XmiI	GTMKAC	1 cut(s)	1178
XmnI	GAANNNNTTC	1 cut(s)	507
XspI	CTAG	3 cut(s)	362, 567, 1511
Zsp2I	ATGCAT	2 cut(s)	34, 74

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.