MD10G1038200.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010685
Physical Location & Seq
Forward (+)
5002031 .. 5005954
3924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1038200.v1.1.491

Sequence Viewer

Length: 624 bp
ATGTCATTGCGCAGACGAACCTTGCTCAAGGTCATCGTTCTCGGCGACAGCGGGGTGGGCAAGACCTCGTTGATGAACCAATATGTGCACAAGAAGTTTAGTCAGCAGTATAAAGCTACAATTGGTGCCGATTTTGTCACCAAAGAACTCCAAATCGATGACAGGCTCGTCACTTTACAAATTTGGGACACGGCCGGGCAGGAAAGATTTCAGAGTCTTGGAGTTGCATTCTACAGAGGAGCAGATTGCTGTGTTCTTGTTTATGATGTTAACGTAATGAAGTCCTTTGATACTCTAGATAATTGGCATGAGGAGTTTCTCAAGCAGGCCGACCCTCCCGATCCCAAGACATTTCCGTTTATCTTGCTTGGTAACAAGATTGATATAGATGGGGGGAACAGTCGTGTGGTGTCGGAGAAGAAAGCAAAGGACTGGTGTGCATCAAAGGGGAATATACCTTATTTTGAGACATCAGCAAAAGAGGATTACAACGTTGATGGTGCATTTCTTTGTATTGCGAAGACTGCTCTTGCAAATGAACATGAGCAGGACATATATTTCCAGGGCGTCCCGGAGGCTGTTACAGAGTCCGATCAGAGAGGAGGTGGCGGCTGTGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

208

Amino Acids

23.09

Weight (kDa)

5.27

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 135 1.2e-11 ADP-ribosylation factor family
Ras PF00071 10 - 176 1.7e-55 Ras family
Roc PF08477 10 - 128 1.3e-33 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 111 1.6e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 10 - 128 9.3e-06 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010685)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09720 AT4G09720 AT4G09720 AT4G09720 AT4G09720 AT4G09720
fragaria_vesca FvH4_2g12800
malus_domestica MD10G1038200.v1.1
prunus_persica Prupe.8G044200_v2.0.a1
pyrus_communis pycom05g02660 pycom10g02620
rosa_chinensis RchiOBHm_Chr6g0273691
rosa_laevigata RLG00000013599
rosa_multiflora Rmu_sc0021426.1_g000003
rosa_roxburghii Rroxscaffold_7G00195120
rosa_rugosa Rorug06G0077300
rosa_samantha Rh6AG191600 Rh6BG195700 Rh6CG195200
rosa_wichuraiana Rw6G016770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
Acc16I TGCGCA 1 cut(s) 11
AccB1I GGYRCC 1 cut(s) 125
AciI CCGC 2 cut(s) 51, 609
AclI AACGTT 1 cut(s) 492
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 1 cut(s) 180
AcyI GRCGYC 1 cut(s) 567
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 461
Alw44I GTGCAC 1 cut(s) 86
AlwI GGATC 1 cut(s) 335
AoxI GGCC 2 cut(s) 192, 327
ApaLI GTGCAC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 12
AsuC2I CCSGG 2 cut(s) 196, 572
AsuHPI GGTGA 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 90
BccI CCATC 2 cut(s) 383, 491
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 1 cut(s) 563
BcnI CCSGG 2 cut(s) 196, 572
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 1 cut(s) 610
BlsI GCNGC 1 cut(s) 611
Bme1390I CCNGG 3 cut(s) 196, 563, 572
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 3 cut(s) 196, 563, 572
BmsI GCATC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 527
BpuEI CTTGAG 2 cut(s) 11, 305
BpuMI CCSGG 2 cut(s) 196, 572
Bsa29I ATCGAT 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 5
BseBI CCWGG 1 cut(s) 563
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 562
BseMI GCAATG 1 cut(s) 5
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 3 cut(s) 252, 326, 615
BseSI GKGCMC 1 cut(s) 90
BseX3I CGGCCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 2 cut(s) 194, 329
BshNI GGYRCC 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 2 cut(s) 195, 572
BslFI GGGAC 2 cut(s) 200, 554
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 2 cut(s) 200, 554
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 2 cut(s) 194, 329
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 340, 592
BspACI CCGC 2 cut(s) 51, 609
BspANI GGCC 2 cut(s) 194, 329
BspDI ATCGAT 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 127
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 5
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 1 cut(s) 562
BssMI GATC 2 cut(s) 340, 592
BssNI GRCGYC 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 1 cut(s) 401
BstACI GRCGYC 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 327, 619
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 2 cut(s) 343, 595
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 2 cut(s) 340, 592
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 3 cut(s) 57, 524, 618
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 3 cut(s) 194, 561, 570
BstSFI CTRYAG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 527
BstZI CGGCCG 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 2 cut(s) 194, 329
BsuTUI ATCGAT 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 327, 619
CfoI GCGC 1 cut(s) 12
ClaI ATCGAT 1 cut(s) 156
CseI GACGC 1 cut(s) 556
CviAII CATG 3 cut(s) 308, 542, 618
CviJI RGCY 6 cut(s) 116, 166, 194, 329, 578, 612
CviKI_1 RGCY 6 cut(s) 116, 166, 194, 329, 578, 612
DpnI GATC 2 cut(s) 342, 594
DpnII GATC 2 cut(s) 340, 592
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
EclXI CGGCCG 1 cut(s) 192
Eco52I CGGCCG 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 561
FaeI CATG 3 cut(s) 311, 545, 621
FaqI GGGAC 2 cut(s) 200, 554
FatI CATG 3 cut(s) 307, 541, 617
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 610
Fsp4HI GCNGC 1 cut(s) 610
FspBI CTAG 1 cut(s) 296
FspI TGCGCA 1 cut(s) 11
GlaI GCGC 1 cut(s) 11
GluI GCNGC 1 cut(s) 610
HaeIII GGCC 2 cut(s) 194, 329
HapII CCGG 2 cut(s) 195, 572
HgaI GACGC 1 cut(s) 556
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 567
Hin1II CATG 3 cut(s) 311, 545, 621
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 2 cut(s) 214, 587
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 2 cut(s) 195, 572
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 2 cut(s) 88, 271
Hpy188I TCNGA 4 cut(s) 213, 415, 592, 597
Hpy188III TCNNGA 2 cut(s) 296, 338
Hpy8I GTNNAC 2 cut(s) 88, 271
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4IV ACGT 2 cut(s) 273, 492
HpyCH4V TGCA 6 cut(s) 88, 227, 440, 503, 533, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 524, 618
HpySE526I ACGT 2 cut(s) 273, 492
Hsp92I GRCGYC 1 cut(s) 567
Hsp92II CATG 3 cut(s) 311, 545, 621
HspAI GCGC 1 cut(s) 10
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 2 cut(s) 340, 592
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 9 cut(s) 148, 185, 208, 311, 418, 533, 548, 575, 585
LweI GCATC 1 cut(s) 449
MaeI CTAG 1 cut(s) 296
MaeII ACGT 2 cut(s) 273, 492
MaeIII GTNAC 4 cut(s) 136, 169, 371, 580
MalI GATC 2 cut(s) 342, 594
MboI GATC 2 cut(s) 340, 592
MboII GAAGA 2 cut(s) 430, 532
MfeI CAATTG 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 3 cut(s) 120, 180, 301
MlyI GAGTC 2 cut(s) 223, 596
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 8 cut(s) 76, 230, 304, 345, 475, 568, 593, 596
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 2 cut(s) 195, 572
MspR9I CCNGG 3 cut(s) 196, 563, 572
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 3 cut(s) 57, 524, 618
NciI CCSGG 2 cut(s) 196, 572
NdeII GATC 2 cut(s) 340, 592
NlaIII CATG 3 cut(s) 311, 545, 621
NlaIV GGNNCC 1 cut(s) 127
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 136, 169
NsbI TGCGCA 1 cut(s) 11
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 207
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 1 cut(s) 611
PleI GAGTC 2 cut(s) 222, 595
PpsI GAGTC 2 cut(s) 222, 595
Psp1406I AACGTT 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 1 cut(s) 127
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 1 cut(s) 610
Sau3AI GATC 2 cut(s) 340, 592
SchI GAGTC 2 cut(s) 223, 596
ScrFI CCNGG 3 cut(s) 196, 563, 572
SduI GDGCHC 1 cut(s) 90
SetI ASST 8 cut(s) 23, 33, 68, 118, 276, 460, 495, 607
SfaNI GCATC 1 cut(s) 449
SfcI CTRYAG 1 cut(s) 232
SmlI CTYRAG 2 cut(s) 26, 320
SmoI CTYRAG 2 cut(s) 26, 320
SphI GCATGC 1 cut(s) 621
Sse9I AATT 3 cut(s) 120, 180, 301
SsiI CCGC 2 cut(s) 51, 609
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 3 cut(s) 194, 561, 570
TaaI ACNGT 1 cut(s) 401
TaiI ACGT 2 cut(s) 276, 495
TaqI TCGA 1 cut(s) 156
TasI AATT 3 cut(s) 120, 180, 301
TauI GCSGC 1 cut(s) 612
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TseFI GTSAC 2 cut(s) 136, 169
Tsp45I GTSAC 2 cut(s) 136, 169
TspDTI ATGAA 3 cut(s) 89, 293, 552
TspGWI ACGGA 1 cut(s) 345
VneI GTGCAC 1 cut(s) 86
XapI RAATTY 1 cut(s) 180
XbaI TCTAGA 1 cut(s) 295
XceI RCATGY 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.