Rorug06G0077300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001075
Physical Location & Seq
Reverse (-)
10331238 .. 10337344
6107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0077300.1

Sequence Viewer

Length: 3300 bp
ATGGAGATCGTCCCGGCGCCGCCAAAGTTACCTGACTGGGACGTCCCAAACGGCGCCGCGTCGCCGCCAGGCACCGTCAAAACGGCGCCGTCGGAGAGCAACAACGAGGCGAACGTGTCGACGTCGTCGGTGGCGGCGAAGTACGACGATGAGGAGGAGGAGGAGGACGTGTGCCGGATCTGTAGGAACCCTGGCGACGCCGATAACCCGCTCAGGTATCCATGCGCGTGTAGCGGAAGCATCAAGTTCGTGCACCAGGATTGTCTCCTTCAGTGGCTCAATCACAGTAATGCTCGCCAATGCGAGGTTTGCAAACATCCATTTTCCTTCTCTCCCGTATATGCTGAGAATGCACCTGCTAGGCTTCCTTTTCAGGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTTTTTCTGCGCCTTAGTTTTGTGCTTTCCGTATGGCTCCTCATTATACCTTTCATTACATTTTGGATATGGCGGCTGGCTTTCGTGAGGAGTTTTGGTGAAGCTCAGAGATTATTCCTAAGTCATCTGTCAACTACAGTCATTCTTACTGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTCATTTTTCTCGGAGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGTCAAGATGTTGACAGAGAAGATGAAGCAGAAAGAAATGGTGCTCGTCCTGTAAGAAGAGTTCCTGGACAAGCTAATAGGAACTTTGTTGGGGACGCTAACGGGGAAGATGCTGCTGGAGCACAAGGGATTGCTGGAGCAGGTCAAATGATTAGAAGGAATGCAGAAAATGTGGCTGCTCGCTGGGAGATGCAGGCAGCTCGTCTTGAGGCTCATGTTGAACAGATGTTTGATGGTCTAGACGATGCCGATGGTGCAGAGGATGTACCATTTGACGAGCTTGTGGGCATGCAGGGACCTGTATTTCATTTAGTGGAAAATGCATTTACTGTACTGGCAAGCAATATGATATTCCTTGGTGTAGTAATATTTGTGCCCTTCTCGTTAGGTCGGATTATACTCTATCATTTATCTTGGCTTTTCTCCACCGCTAGTGGTCCAGTTTTGTCAACAGTCATGCCACTCACTGAATCAGCCCTTTCCCTGGCTAATATGACTTTGAAGAATGCATTAACAGCTTTAACAAATTTATCAACCGATAGCGAAAAAAGTGGTATGGTTGGCCAGGTTGCAGAGATCTTGAAAGTTAACATGAGTGGACTGAATGAGGTTTCGAACAATAATGCAAGTTCACTTTCAGCAGACTTCTTGAAAGGGGCAACAATTGGAACTTCAAGGCTCTCTGATGTGACGACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGCATTGTGGCTTTCATTCGGTACACTAGGGGTGAGCCATTGACTATGGGGAGGTTCTATGGCATTGCTTCAATGGCAGAAACAATTCCATCTCTCTTCAGGCAGTTCCTGGCAGCAATGAGGCATTTGATGACAATGATTAAGGTTGCTTTCCTTCTGGTCATTGAACTTGGAGTGTTCCCACTAATGTGTGGATGGTGGTTGGATGTTTGTACTATAAGGATGTTTGGGAAGTCCATGTCTCACAGGGTGCAGTTCTTCTCAGCATCTCCTTTAGCTAGCTCCCTGGTCCATTGGGTTGTAGGAATCGTATACATGCTGCAAATAAGCATATTCGTCAGTCTTCTTCGAGGGGTTTTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCCAGCTGATCCAAACTACAATCCCTTCCGTGATCTCATTGATGATCCTGTGCACAAACATGCTCGCAGGGTCCTGTTATCCGTTGCCGTGTATGGGAGTTTAATTGTGATGCTGGTGTTTTTACCAGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTTCCTCTTGACATTTCGGTATCTGACCCATTTACTGAAATTCCTGCGGACATGCTTCTATTTCAAATATGCATTCCATTTGCCATTGAGCATTTCAAATTACGGGCAACCATTAAATCCCTGCTCCGTTATTGGTTTACGGCAGTTGGCTGGGCACTTGGTTTAACAGATTTCTTACTGCCCAGACCTGAGGATAACGTTGGACAGGAAAATGGAAATGCAGAGCCTGGAAGACAGGATAGACTACAGGATCAGGCTTTGGTGGCACTTCCAGGTGCTGATGAATCAAATCGAGGCCTTCTTGCATCAGGGGACACAAATGCTGCCGAGGAGTATGACACTGATGAACAATCTGATTCTGAGTATAGCTTTGTGCTTCGAATTGTCCTCTTGTTGGTGGTGGCTTGGATGACTTTACTTGTCTTCAACTCGGCCTTGATAGTTGTACCAACATCACTGGGAAGAACAATTTTCAATGTCATTCCTTTCCTCCCTATAACTCATGGCATCAAGTGCAACGATTTATATGCTTTCATCATCGGAAGCTACATAATATGGACTGCTGTAGCTGGAGTTAGATATTCTATTGAACACATTAGAACAAAAAGGGTTGCAGTTCTATTGGGCCAGATCTGGAAGTGGTGTAGCATTGTCATTAAGAGTTCTGCACTGTTGTCAATATGGATTTTTGTTATTCCAGTATTGATCGGGTTGCTTTTTGAGCTTTTGGTGATTGTGCCGATGCGGGTGCCTGTTGATGAAAGCCCAGTATTCCTGCTGTATCAGGACTGGGCTTTAGGCCTTATTTTTCTCAAGATATGGACTAGATTGGTGATGTTGGATCACATGATGCCATTGGTAGATGAAAGCTGGCGCGTAAAGTTTGAAAGGGTGAGGGAAGATGGCTTCTCCAGGCTGCAGGGTCTTTGGGTGCTCCGAGAGATTGTATTCCCAATCATAATGAAGTTGTTGACTGCCCTGTGCGTGCCTTATGTACTTGCCAGAGGGTTATTTCCTGTGCTCGGTTACCCATTAGTAGTCAACTCAGCAGTGTATCGGTTTGCCTGGATAGGATGCCTGTGCTTTAGTCTGCTGTGCTTCTGTGCCAAGAGGTTCCATGTATGGTTCACCAACCTTCACAACTCTATACGTGATGACCGATATCTAATTGGTCGTAGGCTTCATAACTTTGGAGAAGATGTTGTAGAGAAGCAAAGTGAGGCAGGGACATCCTCTGAATTGCAGGACTCTAGTTTTGAAGTCAGTGGTTTAATTCCTCATGACCGAGAGGCTGACGTTGGTCTCCGTCAAAGGCGTGCTACCCAACATGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

1099

Amino Acids

123.0

Weight (kDa)

5.83

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 58 - 104 9.8e-17 RING-variant domain
MARCHF6_C PF23113 860 - 1038 7.8e-51 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 364
AasI GACNNNNNNGTC 1 cut(s) 41
AatII GACGTC 2 cut(s) 45, 125
Acc36I ACCTGC 2 cut(s) 364, 800
AccB1I GGYRCC 6 cut(s) 16, 53, 71, 85, 1951, 2710
AccBSI CCGCTC 1 cut(s) 211
AccI GTMKAC 2 cut(s) 119, 1725
AccII CGCG 3 cut(s) 59, 227, 2838
AclI AACGTT 1 cut(s) 2160
AclWI GGATC 6 cut(s) 185, 1796, 1805, 1841, 2220, 2811
AcoI YGGCCR 1 cut(s) 1231
AcsI RAATTY 2 cut(s) 1195, 2001
AcuI CTGAAG 3 cut(s) 254, 631, 1495
AcyI GRCGYC 6 cut(s) 17, 42, 54, 86, 122, 198
AdeI CACNNNGTG 1 cut(s) 1663
AfaI GTAC 7 cut(s) 143, 936, 1002, 1436, 1627, 2409, 2958
AfiI CCNNNNNNNGG 5 cut(s) 304, 1153, 1896, 2356, 2984
AflIII ACRYGT 3 cut(s) 114, 168, 1380
AjiI CACGTC 1 cut(s) 169
AleI CACNNNNGTG 1 cut(s) 1600
Alw21I GWGCWC 6 cut(s) 255, 715, 793, 1857, 2898, 2985
Alw26I GTCTC 3 cut(s) 269, 1659, 3269
Alw44I GTGCAC 2 cut(s) 251, 1853
AlwI GGATC 6 cut(s) 185, 1796, 1805, 1841, 2220, 2811
AlwNI CAGNNNCTG 3 cut(s) 1522, 2189, 2240
AoxI GGCC 5 cut(s) 1231, 2257, 2394, 2587, 2761
ApaLI GTGCAC 2 cut(s) 251, 1853
ApeKI GCWGC 7 cut(s) 782, 845, 866, 1526, 1732, 2285, 2878
ApoI RAATTY 2 cut(s) 1195, 2001
ArsI GACNNNNNNTTYG 2 cut(s) 3200, 3232
Asp700I GAANNNNTTC 1 cut(s) 1497
AspLEI GCGC 6 cut(s) 19, 56, 88, 227, 432, 2838
AspS9I GGNCC 5 cut(s) 965, 1106, 1702, 1873, 2587
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 6 cut(s) 530, 1457, 2704, 2806, 2866, 3082
AsuII TTCGAA 2 cut(s) 1283, 2341
AsuNHI GCTAGC 2 cut(s) 599, 1691
AvaII GGWCC 4 cut(s) 965, 1106, 1702, 1873
AxyI CCTNAGG 1 cut(s) 2151
BaeGI GKGCMC 4 cut(s) 255, 1047, 1857, 2119
BalI TGGCCA 1 cut(s) 1233
BanI GGYRCC 6 cut(s) 16, 53, 71, 85, 1951, 2710
BbsI GAAGAC 3 cut(s) 1748, 2200, 2377
Bbv12I GWGCWC 6 cut(s) 255, 715, 793, 1857, 2898, 2985
BbvI GCAGC 7 cut(s) 769, 832, 878, 1538, 1719, 2272, 2865
BccI CCATC 7 cut(s) 385, 896, 914, 1510, 1602, 1942, 2858
BceAI ACGGC 5 cut(s) 67, 73, 99, 1874, 2118
BcgI CGANNNNNNTGC 8 cut(s) 229, 263, 851, 885, 2471, 2505, 2692, 2726
BciVI GTATCC 2 cut(s) 228, 1370
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 3 cut(s) 269, 1659, 3269
BfmI CTRYAG 5 cut(s) 181, 555, 2207, 2526, 2879
BfoI RGCGCY 3 cut(s) 20, 57, 89
BfuAI ACCTGC 2 cut(s) 364, 800
BfuI GTATCC 2 cut(s) 228, 1370
BglII AGATCT 2 cut(s) 1245, 2592
Bme18I GGWCC 4 cut(s) 965, 1106, 1702, 1873
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 5 cut(s) 965, 1106, 1702, 1873, 2587
BmrI ACTGGG 4 cut(s) 46, 2429, 2723, 2761
BmtI GCTAGC 2 cut(s) 603, 1695
BmuI ACTGGG 4 cut(s) 46, 2429, 2723, 2761
BoxI GACNNNNGTC 1 cut(s) 3261
BpiI GAAGAC 3 cut(s) 1748, 2200, 2377
BpmI CTGGAG 4 cut(s) 807, 825, 2553, 2857
Bpu10I CCTNAGC 1 cut(s) 212
Bpu14I TTCGAA 2 cut(s) 1283, 2341
BpuEI CTTGAG 2 cut(s) 896, 2759
BpuMI CCSGG 1 cut(s) 14
BsaAI YACGTR 2 cut(s) 659, 3113
BsaHI GRCGYC 6 cut(s) 17, 42, 54, 86, 122, 198
BsaI GGTCTC 1 cut(s) 3269
BsaJI CCNNGG 6 cut(s) 190, 1024, 1152, 1411, 1698, 2289
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 502, 532, 3147, 3177
Bsc4I CCNNNNNNNGG 5 cut(s) 304, 1153, 1896, 2356, 2984
Bse1I ACTGG 8 cut(s) 41, 1008, 1109, 1928, 2424, 2660, 2729, 2756
Bse21I CCTNAGG 1 cut(s) 2151
Bse3DI GCAATG 3 cut(s) 402, 1476, 1536
BseDI CCNNGG 6 cut(s) 190, 1024, 1152, 1411, 1698, 2289
BseLI CCNNNNNNNGG 5 cut(s) 304, 1153, 1896, 2356, 2984
BseMI GCAATG 3 cut(s) 402, 1476, 1536
BseMII CTCAG 8 cut(s) 226, 336, 539, 626, 1689, 2142, 2313, 3021
BseNI ACTGG 8 cut(s) 41, 1008, 1109, 1928, 2424, 2660, 2729, 2756
BseRI GAGGAG 7 cut(s) 167, 170, 173, 176, 449, 523, 2306
BseSI GKGCMC 4 cut(s) 255, 1047, 1857, 2119
BseXI GCAGC 7 cut(s) 769, 832, 878, 1538, 1719, 2272, 2865
BseYI CCCAGC 2 cut(s) 852, 2112
BsgI GTGCAG 3 cut(s) 945, 1685, 2613
Bsh1236I CGCG 3 cut(s) 59, 227, 2838
BshFI GGCC 5 cut(s) 1233, 2259, 2396, 2589, 2763
BshNI GGYRCC 6 cut(s) 16, 53, 71, 85, 1951, 2710
BsiHKAI GWGCWC 6 cut(s) 255, 715, 793, 1857, 2898, 2985
BsiSI CCGG 2 cut(s) 14, 175
BslFI GGGAC 6 cut(s) 29, 53, 776, 978, 2288, 3202
BslI CCNNNNNNNGG 5 cut(s) 304, 1153, 1896, 2356, 2984
BsmAI GTCTC 3 cut(s) 269, 1659, 3269
BsmFI GGGAC 6 cut(s) 29, 53, 776, 978, 2288, 3202
BsmI GAATGC 4 cut(s) 355, 835, 1180, 2034
BsnI GGCC 5 cut(s) 1233, 2259, 2396, 2589, 2763
Bso31I GGTCTC 1 cut(s) 3269
Bsp119I TTCGAA 2 cut(s) 1283, 2341
Bsp1286I GDGCHC 8 cut(s) 255, 715, 793, 1047, 1857, 2119, 2898, 2985
BspANI GGCC 5 cut(s) 1233, 2259, 2396, 2589, 2763
BspCNI CTCAG 8 cut(s) 225, 337, 538, 627, 1688, 2143, 2314, 3020
BspFNI CGCG 3 cut(s) 59, 227, 2838
BspHI TCATGA 1 cut(s) 3241
BspMAI CTGCAG 1 cut(s) 2883
BspMI ACCTGC 2 cut(s) 364, 800
BspOI GCTAGC 2 cut(s) 603, 1695
BspPI GGATC 6 cut(s) 185, 1796, 1805, 1841, 2220, 2811
BspT104I TTCGAA 2 cut(s) 1283, 2341
BspT107I GGYRCC 6 cut(s) 16, 53, 71, 85, 1951, 2710
BspTNI GGTCTC 1 cut(s) 3269
BsrBI CCGCTC 1 cut(s) 211
BsrDI GCAATG 3 cut(s) 402, 1476, 1536
BsrI ACTGG 8 cut(s) 41, 1008, 1109, 1928, 2424, 2660, 2729, 2756
BssECI CCNNGG 6 cut(s) 190, 1024, 1152, 1411, 1698, 2289
BssNAI GTATAC 1 cut(s) 1726
BssNI GRCGYC 6 cut(s) 17, 42, 54, 86, 122, 198
BssT1I CCWWGG 2 cut(s) 1024, 1411
Bst1107I GTATAC 1 cut(s) 1726
Bst4CI ACNGT 6 cut(s) 76, 287, 559, 1000, 1123, 2634
Bst6I CTCTTC 2 cut(s) 721, 1514
BstACI GRCGYC 6 cut(s) 17, 42, 54, 86, 122, 198
BstAPI GCANNNNNTGC 1 cut(s) 2475
BstBAI YACGTR 2 cut(s) 659, 3113
BstBI TTCGAA 2 cut(s) 1283, 2341
BstEII GGTNACC 1 cut(s) 2987
BstFNI CGCG 3 cut(s) 59, 227, 2838
BstH2I RGCGCY 3 cut(s) 20, 57, 89
BstHHI GCGC 6 cut(s) 19, 56, 88, 227, 432, 2838
BstMAI GTCTC 3 cut(s) 269, 1659, 3269
BstNSI RCATGY 5 cut(s) 961, 1384, 1732, 1865, 2017
BstPAI GACNNNNGTC 1 cut(s) 3261
BstPI GGTNACC 1 cut(s) 2987
BstSFI CTRYAG 5 cut(s) 181, 555, 2207, 2526, 2879
BstSLI GKGCMC 4 cut(s) 255, 1047, 1857, 2119
BstUI CGCG 3 cut(s) 59, 227, 2838
BstV1I GCAGC 7 cut(s) 769, 832, 878, 1538, 1719, 2272, 2865
BstV2I GAAGAC 3 cut(s) 1748, 2200, 2377
BstX2I RGATCY 4 cut(s) 177, 1245, 1801, 2592
BstYI RGATCY 4 cut(s) 177, 1245, 1801, 2592
BstZ17I GTATAC 1 cut(s) 1726
Bsu36I CCTNAGG 1 cut(s) 2151
BsuI GTATCC 2 cut(s) 228, 1370
BsuRI GGCC 5 cut(s) 1233, 2259, 2396, 2589, 2763
BtrI CACGTC 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 3018
BtsIMutI CAGTG 7 cut(s) 278, 1134, 2301, 2417, 2630, 3018, 3232
BveI ACCTGC 2 cut(s) 364, 800
CaiI CAGNNNCTG 3 cut(s) 1522, 2189, 2240
CciI TCATGA 1 cut(s) 3241
CfoI GCGC 6 cut(s) 19, 56, 88, 227, 432, 2838
Cfr13I GGNCC 5 cut(s) 965, 1106, 1702, 1873, 2587
CseI GACGC 3 cut(s) 48, 206, 773
Csp6I GTAC 7 cut(s) 142, 935, 1001, 1435, 1626, 2408, 2957
CviQI GTAC 7 cut(s) 142, 935, 1001, 1435, 1626, 2408, 2957
DinI GGCGCC 3 cut(s) 18, 55, 87
DraIII CACNNNGTG 1 cut(s) 1663
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 1231
Eam1104I CTCTTC 2 cut(s) 721, 1514
EarI CTCTTC 2 cut(s) 721, 1514
Eco130I CCWWGG 2 cut(s) 1024, 1411
Eco147I AGGCCT 2 cut(s) 2259, 2763
Eco31I GGTCTC 1 cut(s) 3269
Eco32I GATATC 1 cut(s) 3125
Eco47I GGWCC 4 cut(s) 965, 1106, 1702, 1873
Eco57I CTGAAG 3 cut(s) 254, 631, 1495
Eco81I CCTNAGG 1 cut(s) 2151
Eco91I GGTNACC 1 cut(s) 2987
EcoO109I RGGNCCY 2 cut(s) 965, 1873
EcoO65I GGTNACC 1 cut(s) 2987
EcoRV GATATC 1 cut(s) 3125
EcoT14I CCWWGG 2 cut(s) 1024, 1411
EcoT22I ATGCAT 3 cut(s) 994, 1180, 2036
EgeI GGCGCC 3 cut(s) 18, 55, 87
EheI GGCGCC 3 cut(s) 18, 55, 87
ErhI CCWWGG 2 cut(s) 1024, 1411
FaqI GGGAC 6 cut(s) 29, 53, 776, 978, 2288, 3202
FauI CCCGC 2 cut(s) 216, 2700
FblI GTMKAC 2 cut(s) 119, 1725
GlaI GCGC 6 cut(s) 18, 55, 87, 226, 431, 2837
GsaI CCCAGC 2 cut(s) 856, 2116
GsuI CTGGAG 4 cut(s) 807, 825, 2553, 2857
HaeII RGCGCY 3 cut(s) 20, 57, 89
HaeIII GGCC 5 cut(s) 1233, 2259, 2396, 2589, 2763
HapII CCGG 2 cut(s) 14, 175
HgaI GACGC 3 cut(s) 48, 206, 773
HhaI GCGC 6 cut(s) 19, 56, 88, 227, 432, 2838
Hin1I GRCGYC 6 cut(s) 17, 42, 54, 86, 122, 198
Hin6I GCGC 6 cut(s) 17, 54, 86, 225, 430, 2836
HinP1I GCGC 6 cut(s) 17, 54, 86, 225, 430, 2836
HincII GTYRAC 7 cut(s) 120, 552, 682, 1119, 1258, 2934, 3004
HindII GTYRAC 7 cut(s) 120, 552, 682, 1119, 1258, 2934, 3004
HinfI GANTC 6 cut(s) 1139, 1363, 1719, 2246, 2318, 3209
HpaI GTTAAC 1 cut(s) 1258
HpaII CCGG 2 cut(s) 14, 175
HphI GGTGA 6 cut(s) 530, 1457, 2704, 2806, 2866, 3082
Hpy99I CGWCG 7 cut(s) 64, 94, 124, 127, 130, 149, 200
HpyAV CCTTC 9 cut(s) 278, 337, 819, 1057, 1577, 1837, 1965, 2270, 3107
HpyCH4III ACNGT 6 cut(s) 76, 287, 559, 1000, 1123, 2634
HpyCH4IV ACGT 8 cut(s) 42, 114, 122, 168, 658, 2160, 3112, 3258
HpySE526I ACGT 8 cut(s) 42, 114, 122, 168, 658, 2160, 3112, 3258
Hsp92I GRCGYC 6 cut(s) 17, 42, 54, 86, 122, 198
HspAI GCGC 6 cut(s) 17, 54, 86, 225, 430, 2836
KasI GGCGCC 3 cut(s) 16, 53, 85
KspAI GTTAAC 1 cut(s) 1258
LmnI GCTCC 7 cut(s) 462, 623, 788, 806, 1700, 2091, 2901
Lsp1109I GCAGC 7 cut(s) 769, 832, 878, 1538, 1719, 2272, 2865
MaeII ACGT 8 cut(s) 42, 114, 122, 168, 658, 2160, 3112, 3258
MaeIII GTNAC 3 cut(s) 27, 1357, 2987
MbiI CCGCTC 1 cut(s) 211
MfeI CAATTG 1 cut(s) 1332
MflI RGATCY 4 cut(s) 177, 1245, 1801, 2592
MhlI GDGCHC 8 cut(s) 255, 715, 793, 1047, 1857, 2119, 2898, 2985
MlsI TGGCCA 1 cut(s) 1233
MluNI TGGCCA 1 cut(s) 1233
Mly113I GGCGCC 3 cut(s) 17, 54, 86
MlyI GAGTC 2 cut(s) 1357, 3203
MmeI TCCRAC 5 cut(s) 72, 1040, 1596, 2143, 2781
Mox20I TGGCCA 1 cut(s) 1233
Mph1103I ATGCAT 3 cut(s) 994, 1180, 2036
MroXI GAANNNNTTC 1 cut(s) 1497
MscI TGGCCA 1 cut(s) 1233
MslI CAYNNNNRTG 5 cut(s) 226, 288, 657, 1600, 1860
Msp20I TGGCCA 1 cut(s) 1233
MspA1I CMGCKG 1 cut(s) 1808
MspI CCGG 2 cut(s) 14, 175
MunI CAATTG 1 cut(s) 1332
Mva1269I GAATGC 4 cut(s) 355, 835, 1180, 2034
MvnI CGCG 3 cut(s) 59, 227, 2838
NarI GGCGCC 3 cut(s) 17, 54, 86
NciI CCSGG 1 cut(s) 14
NheI GCTAGC 2 cut(s) 599, 1691
NmeAIII GCCGAG 2 cut(s) 2314, 2372
NmuCI GTSAC 1 cut(s) 1357
NsiI ATGCAT 3 cut(s) 994, 1180, 2036
NspI RCATGY 5 cut(s) 961, 1384, 1732, 1865, 2017
NspV TTCGAA 2 cut(s) 1283, 2341
OliI CACNNNNGTG 1 cut(s) 1600
PaeI GCATGC 1 cut(s) 961
PagI TCATGA 1 cut(s) 3241
PaqCI CACCTGC 1 cut(s) 364
PceI AGGCCT 2 cut(s) 2259, 2763
PciI ACATGT 1 cut(s) 1380
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 134, 3118
PctI GAATGC 4 cut(s) 355, 835, 1180, 2034
PdmI GAANNNNTTC 1 cut(s) 1497
PfeI GAWTC 4 cut(s) 1139, 1719, 2246, 2318
PflFI GACNNNGTC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 733
PleI GAGTC 2 cut(s) 1357, 3203
PluTI GGCGCC 3 cut(s) 20, 57, 89
PpsI GAGTC 2 cut(s) 1357, 3203
Ppu21I YACGTR 2 cut(s) 659, 3113
PpuMI RGGWCCY 2 cut(s) 965, 1873
PscI ACATGT 1 cut(s) 1380
PshAI GACNNNNGTC 1 cut(s) 3261
Psp1406I AACGTT 1 cut(s) 2160
Psp5II RGGWCCY 2 cut(s) 965, 1873
PspEI GGTNACC 1 cut(s) 2987
PspFI CCCAGC 2 cut(s) 852, 2112
PspPI GGNCC 5 cut(s) 965, 1106, 1702, 1873, 2587
PspPPI RGGWCCY 2 cut(s) 965, 1873
PsrI GAACNNNNNNTAC 2 cut(s) 714, 746
PstI CTGCAG 1 cut(s) 2883
PstNI CAGNNNCTG 3 cut(s) 1522, 2189, 2240
PsuI RGATCY 4 cut(s) 177, 1245, 1801, 2592
PsyI GACNNNGTC 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 1808
RsaI GTAC 7 cut(s) 143, 936, 1002, 1436, 1627, 2409, 2958
RsaNI GTAC 7 cut(s) 142, 935, 1001, 1435, 1626, 2408, 2957
RseI CAYNNNNRTG 5 cut(s) 226, 288, 657, 1600, 1860
SalI GTCGAC 1 cut(s) 118
Sau96I GGNCC 5 cut(s) 965, 1106, 1702, 1873, 2587
SchI GAGTC 2 cut(s) 1357, 3203
SduI GDGCHC 8 cut(s) 255, 715, 793, 1047, 1857, 2119, 2898, 2985
SfcI CTRYAG 5 cut(s) 181, 555, 2207, 2526, 2879
SfoI GGCGCC 3 cut(s) 18, 55, 87
SfuI TTCGAA 2 cut(s) 1283, 2341
SinI GGWCC 4 cut(s) 965, 1106, 1702, 1873
SmiMI CAYNNNNRTG 5 cut(s) 226, 288, 657, 1600, 1860
SmlI CTYRAG 2 cut(s) 875, 2774
SmoI CTYRAG 2 cut(s) 875, 2774
SphI GCATGC 1 cut(s) 961
SseBI AGGCCT 2 cut(s) 2259, 2763
SspDI GGCGCC 3 cut(s) 16, 53, 85
SspI AATATT 1 cut(s) 1038
StuI AGGCCT 2 cut(s) 2259, 2763
StyI CCWWGG 2 cut(s) 1024, 1411
TaaI ACNGT 6 cut(s) 76, 287, 559, 1000, 1123, 2634
TaiI ACGT 8 cut(s) 45, 117, 125, 171, 661, 2163, 3115, 3261
TaqI TCGA 5 cut(s) 119, 1283, 1762, 2254, 2341
TaqII GACCGA 2 cut(s) 3135, 3261
TatI WGTACW 3 cut(s) 1000, 1625, 2956
TauI GCSGC 5 cut(s) 22, 59, 67, 137, 496
TfiI GAWTC 4 cut(s) 1139, 1719, 2246, 2318
TscAI CASTG 7 cut(s) 278, 1141, 2308, 2424, 2637, 3018, 3232
TseFI GTSAC 1 cut(s) 1357
TseI GCWGC 7 cut(s) 782, 845, 866, 1526, 1732, 2285, 2878
Tsp45I GTSAC 1 cut(s) 1357
TspGWI ACGGA 5 cut(s) 439, 1820, 1873, 2078, 3257
TspRI CASTG 7 cut(s) 278, 1141, 2308, 2424, 2637, 3018, 3232
Tth111I GACNNNGTC 1 cut(s) 124
VneI GTGCAC 2 cut(s) 251, 1853
VpaK11BI GGWCC 4 cut(s) 965, 1106, 1702, 1873
XapI RAATTY 2 cut(s) 1195, 2001
XbaI TCTAGA 1 cut(s) 907
XceI RCATGY 5 cut(s) 961, 1384, 1732, 1865, 2017
XmiI GTMKAC 2 cut(s) 119, 1725
XmnI GAANNNNTTC 1 cut(s) 1497
ZraI GACGTC 2 cut(s) 43, 123
Zsp2I ATGCAT 3 cut(s) 994, 1180, 2036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.