Rroxscaffold_7G00195090

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001075
Physical Location & Seq
Forward (+)
37292028 .. 37295748
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195090.1

Sequence Viewer

Length: 1686 bp
ATGGGTGTGCTCCAACGATTGCTGGGTTCTCTGCTTCTATGCACTGCGCTTCTTTGCCTAGTTGAAGGAAATGTTCACTACTATGACTTTGTACTCAAGGAGGCCAACTTTACAAGGTTATGTCGGACTAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGTCATTCGTGTGCAAAAAGGTGATACTGTTTATGTCAATGTCCACAATCAAGGAGACTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCAAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACACAGTGTCCAATTGAACCCAGATCAAATTTCACTTACGAGGTTATATTTTCTGATGAGGAAGGAACAGTTTGGTGGCATGCTCACAGTGATTGGACCAGAGCTAGTATCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACGGGTTTTCCATTTCCAGAGCCAGATGGAGAAGAAATAGTTGTACTTGGTTCTTGGTATTACATTGATAATCTGAATGAGGTGGTTGCTGAGGATGTCGAAGATGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTCGGCTTATGCTCAAATGATAGTACATATCGCTGGCAAGTGGAGTATGGCAAGACATACCTTCTTCGCCTGGTTAGTGCAGTTATGAATGCAGAACTTTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTTAAACCAATTGTGACCGACAATATCATGATTTCCCCCGGACAAACCATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGTGTTAGGCCAGATGATCAAGAATATGACAAGTCAAATGTTACTGCAATTCTGGAGTATAAAGGCAATTACACAAGCCCGGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCAATAGAATTTACAAACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGATGATGTGACAGTTTCTCTTCTTGCTAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGACAATTTGCCTGATAATACTGAGGTAACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATAGTGTTTCAGGGTACTAATACGATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGCTATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGACAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTGTATCAGTCCCAATGAAAGGATGGCTTGCTATCAGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGAGGCACACCGGAGACGAGCATGCGAGAACCTCCTCCAAACATGCCTCCCTGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

561

Amino Acids

62.93

Weight (kDa)

4.67

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 146 1.9e-39 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 1.1e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 425 - 518 1.1e-22 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1006
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 1091
AclWI GGATC 4 cut(s) 1450, 1469, 1493, 1588
AcsI RAATTY 3 cut(s) 325, 1007, 1456
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 7 cut(s) 93, 486, 625, 970, 1085, 1343, 1375
AfiI CCNNNNNNNGG 2 cut(s) 1212, 1535
AgsI TTSAA 2 cut(s) 65, 314
AjnI CCWGG 3 cut(s) 160, 592, 669
AluBI AGCT 7 cut(s) 401, 821, 1029, 1173, 1428, 1485, 1606
AluI AGCT 7 cut(s) 401, 821, 1029, 1173, 1428, 1485, 1606
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 4 cut(s) 216, 434, 834, 1635
AlwI GGATC 4 cut(s) 1450, 1469, 1493, 1588
AoxI GGCC 4 cut(s) 102, 164, 229, 875
ApeKI GCWGC 1 cut(s) 851
ApoI RAATTY 3 cut(s) 325, 1007, 1456
AseI ATTAAT 1 cut(s) 1386
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 164, 284, 393
AsuC2I CCSGG 2 cut(s) 792, 947
AsuHPI GGTGA 2 cut(s) 200, 1075
AvaII GGWCC 2 cut(s) 284, 393
BaeI ACNNNNGTAYC 2 cut(s) 1503, 1536
BanI GGYRCC 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 961, 993
Bbv12I GWGCWC 1 cut(s) 12
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 838
BccI CCATC 5 cut(s) 275, 461, 539, 737, 1533
BciT130I CCWGG 3 cut(s) 162, 594, 671
BciVI GTATCC 1 cut(s) 416
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 2 cut(s) 792, 947
BcoDI GTCTC 4 cut(s) 216, 434, 834, 1635
BfaI CTAG 4 cut(s) 59, 402, 855, 1155
BfmI CTRYAG 3 cut(s) 1188, 1210, 1423
BfuAI ACCTGC 1 cut(s) 1006
BfuI GTATCC 1 cut(s) 416
BisI GCNGC 1 cut(s) 852
BlpI GCTNAGC 1 cut(s) 1030
BlsI GCNGC 1 cut(s) 853
Bme1390I CCNGG 5 cut(s) 162, 594, 671, 792, 947
Bme18I GGWCC 2 cut(s) 284, 393
BmgT120I GGNCC 3 cut(s) 164, 284, 393
BmiI GGNNCC 2 cut(s) 269, 415
BmrFI CCNGG 5 cut(s) 162, 594, 671, 792, 947
BmsI GCATC 2 cut(s) 265, 1400
BpmI CTGGAG 1 cut(s) 941
Bpu10I CCTNAGC 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 1030
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 792, 947
BsaJI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 801, 1323
BsaWI WCCGGW 1 cut(s) 1637
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 2 cut(s) 1212, 1535
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 3 cut(s) 167, 248, 826
BseBI CCWGG 3 cut(s) 162, 594, 671
BseDI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 801, 1323
BseGI GGATG 4 cut(s) 541, 748, 1402, 1544
BseLI CCNNNNNNNGG 2 cut(s) 1212, 1535
BseMII CTCAG 4 cut(s) 279, 522, 1251, 1293
BseNI ACTGG 3 cut(s) 167, 248, 826
BseRI GAGGAG 2 cut(s) 549, 1651
BseXI GCAGC 1 cut(s) 838
BseYI CCCAGC 2 cut(s) 22, 1173
BsgI GTGCAG 2 cut(s) 699, 1664
BshFI GGCC 4 cut(s) 104, 166, 231, 877
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 4 cut(s) 792, 947, 1061, 1638
BslFI GGGAC 1 cut(s) 1511
BslI CCNNNNNNNGG 2 cut(s) 1212, 1535
BsmAI GTCTC 4 cut(s) 216, 434, 834, 1635
BsmBI CGTCTC 2 cut(s) 434, 1635
BsmFI GGGAC 1 cut(s) 1511
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 4 cut(s) 104, 166, 231, 877
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1407I TGTACA 1 cut(s) 1083
Bsp143I GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
Bsp1720I GCTNAGC 1 cut(s) 1030
Bsp19I CCATGG 2 cut(s) 409, 801
BspACI CCGC 1 cut(s) 1091
BspANI GGCC 4 cut(s) 104, 166, 231, 877
BspCNI CTCAG 4 cut(s) 280, 523, 1250, 1294
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 2 cut(s) 269, 415
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 4 cut(s) 1450, 1469, 1493, 1588
BspT107I GGYRCC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 1060
BsrGI TGTACA 1 cut(s) 1083
BsrI ACTGG 3 cut(s) 167, 248, 826
BssAI RCCGGY 1 cut(s) 1060
BssECI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 801, 1323
BssMI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
BssT1I CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
Bst2UI CCWGG 3 cut(s) 162, 594, 671
Bst6I CTCTTC 2 cut(s) 1152, 1344
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 1083
BstC8I GCNNGC 6 cut(s) 137, 378, 635, 1036, 1545, 1650
BstDEI CTNAG 6 cut(s) 129, 288, 531, 1030, 1237, 1302
BstDSI CCRYGG 2 cut(s) 409, 801
BstENI CCTNNNNNAGG 1 cut(s) 1210
BstF5I GGATG 4 cut(s) 541, 748, 1402, 1544
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 6 cut(s) 323, 886, 1445, 1477, 1501, 1583
BstMAI GTCTC 4 cut(s) 216, 434, 834, 1635
BstMBI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
BstMWI GCNNNNNNNGC 3 cut(s) 253, 570, 1035
BstNI CCWGG 3 cut(s) 162, 594, 671
BstNSI RCATGY 4 cut(s) 139, 380, 1652, 1673
BstSCI CCNGG 5 cut(s) 160, 592, 669, 790, 945
BstSFI CTRYAG 3 cut(s) 1188, 1210, 1423
BstV1I GCAGC 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 1474
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 1474
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 4 cut(s) 104, 166, 231, 877
BtgI CCRYGG 2 cut(s) 409, 801
BtsCI GGATG 4 cut(s) 541, 748, 1402, 1544
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 8 cut(s) 42, 241, 308, 391, 726, 1515, 1568, 1681
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 6 cut(s) 137, 378, 635, 1036, 1545, 1650
CciI TCATGA 1 cut(s) 780
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 1060
Cfr13I GGNCC 3 cut(s) 164, 284, 393
Csp6I GTAC 7 cut(s) 92, 485, 624, 969, 1084, 1342, 1374
CviQI GTAC 7 cut(s) 92, 485, 624, 969, 1084, 1342, 1374
DdeI CTNAG 6 cut(s) 129, 288, 531, 1030, 1237, 1302
DpnI GATC 6 cut(s) 322, 885, 1444, 1476, 1500, 1582
DpnII GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
DraI TTTAAA 1 cut(s) 1161
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 1152, 1344
EarI CTCTTC 2 cut(s) 1152, 1344
Eco130I CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
Eco47I GGWCC 2 cut(s) 284, 393
EcoNI CCTNNNNNAGG 1 cut(s) 1210
EcoRII CCWGG 3 cut(s) 160, 592, 669
EcoT14I CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
EcoT22I ATGCAT 1 cut(s) 1393
ErhI CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
Esp3I CGTCTC 2 cut(s) 434, 1635
FalI AAGNNNNNCTT 4 cut(s) 256, 288, 1527, 1559
FaqI GGGAC 1 cut(s) 1511
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 1 cut(s) 852
FokI GGATG 4 cut(s) 548, 755, 1389, 1551
Fsp4HI GCNGC 1 cut(s) 852
FspBI CTAG 4 cut(s) 59, 402, 855, 1155
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 852
GsaI CCCAGC 2 cut(s) 26, 1177
GsuI CTGGAG 1 cut(s) 941
HaeIII GGCC 4 cut(s) 104, 166, 231, 877
HapII CCGG 4 cut(s) 792, 947, 1061, 1638
HhaI GCGC 1 cut(s) 49
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 1 cut(s) 1399
HpaII CCGG 4 cut(s) 792, 947, 1061, 1638
HphI GGTGA 2 cut(s) 200, 1075
Hpy166II GTNNAC 2 cut(s) 76, 211
Hpy188III TCNNGA 6 cut(s) 287, 458, 781, 887, 920, 1024
Hpy8I GTNNAC 2 cut(s) 76, 211
HpyAV CCTTC 4 cut(s) 59, 353, 671, 987
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 570, 1035
HpyF3I CTNAG 6 cut(s) 129, 288, 531, 1030, 1237, 1302
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
LmnI GCTCC 2 cut(s) 15, 746
Lsp1109I GCAGC 1 cut(s) 838
LweI GCATC 2 cut(s) 265, 1400
MaeI CTAG 4 cut(s) 59, 402, 855, 1155
MaeIII GTNAC 6 cut(s) 295, 766, 814, 907, 1135, 1306
MalI GATC 6 cut(s) 322, 885, 1444, 1476, 1500, 1582
MboI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
MboII GAAGA 7 cut(s) 485, 554, 656, 1139, 1361, 1519, 1634
MfeI CAATTG 2 cut(s) 309, 762
MflI RGATCY 1 cut(s) 1474
MhlI GDGCHC 1 cut(s) 12
MmeI TCCRAC 4 cut(s) 37, 104, 1208, 1420
Mph1103I ATGCAT 1 cut(s) 1393
MseI TTAA 4 cut(s) 756, 1160, 1179, 1386
MslI CAYNNNNRTG 4 cut(s) 81, 176, 246, 1416
MspI CCGG 4 cut(s) 792, 947, 1061, 1638
MspR9I CCNGG 5 cut(s) 162, 594, 671, 792, 947
MssI GTTTAAAC 1 cut(s) 1161
MunI CAATTG 2 cut(s) 309, 762
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 3 cut(s) 162, 594, 671
MwoI GCNNNNNNNGC 3 cut(s) 253, 570, 1035
NciI CCSGG 2 cut(s) 792, 947
NcoI CCATGG 2 cut(s) 409, 801
NdeI CATATG 1 cut(s) 751
NdeII GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
NlaIV GGNNCC 2 cut(s) 269, 415
NmuCI GTSAC 4 cut(s) 295, 766, 814, 1135
NsiI ATGCAT 1 cut(s) 1393
NspI RCATGY 4 cut(s) 139, 380, 1652, 1673
PaeI GCATGC 3 cut(s) 139, 380, 1652
PagI TCATGA 1 cut(s) 780
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 1 cut(s) 1399
PkrI GCNGC 1 cut(s) 853
PmeI GTTTAAAC 1 cut(s) 1161
PshBI ATTAAT 1 cut(s) 1386
Psp6I CCWGG 3 cut(s) 160, 592, 669
PspFI CCCAGC 2 cut(s) 22, 1173
PspGI CCWGG 3 cut(s) 160, 592, 669
PspN4I GGNNCC 2 cut(s) 269, 415
PspPI GGNCC 3 cut(s) 164, 284, 393
PsuI RGATCY 1 cut(s) 1474
RsaI GTAC 7 cut(s) 93, 486, 625, 970, 1085, 1343, 1375
RsaNI GTAC 7 cut(s) 92, 485, 624, 969, 1084, 1342, 1374
RseI CAYNNNNRTG 4 cut(s) 81, 176, 246, 1416
SaqAI TTAA 4 cut(s) 756, 1160, 1179, 1386
SatI GCNGC 1 cut(s) 852
Sau3AI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1580
Sau96I GGNCC 3 cut(s) 164, 284, 393
ScrFI CCNGG 5 cut(s) 162, 594, 671, 792, 947
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 2 cut(s) 265, 1400
SfcI CTRYAG 3 cut(s) 1188, 1210, 1423
SinI GGWCC 2 cut(s) 284, 393
SmiMI CAYNNNNRTG 4 cut(s) 81, 176, 246, 1416
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SphI GCATGC 3 cut(s) 139, 380, 1652
SsiI CCGC 1 cut(s) 1091
SspMI CTAG 4 cut(s) 59, 402, 855, 1155
StyD4I CCNGG 5 cut(s) 160, 592, 669, 790, 945
StyI CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
TaqI TCGA 2 cut(s) 540, 1583
TaqII GACCGA 1 cut(s) 785
TatI WGTACW 5 cut(s) 91, 484, 623, 1083, 1341
TfiI GAWTC 1 cut(s) 1399
Tru1I TTAA 4 cut(s) 756, 1160, 1179, 1386
Tru9I TTAA 4 cut(s) 756, 1160, 1179, 1386
TscAI CASTG 7 cut(s) 49, 248, 308, 391, 733, 1522, 1575
TseFI GTSAC 4 cut(s) 295, 766, 814, 1135
TseI GCWGC 1 cut(s) 851
Tsp45I GTSAC 4 cut(s) 295, 766, 814, 1135
TspDTI ATGAA 5 cut(s) 596, 701, 1115, 1362, 1547
TspRI CASTG 7 cut(s) 49, 248, 308, 391, 733, 1522, 1575
VpaK11BI GGWCC 2 cut(s) 284, 393
VspI ATTAAT 1 cut(s) 1386
XagI CCTNNNNNAGG 1 cut(s) 1210
XapI RAATTY 3 cut(s) 325, 1007, 1456
XceI RCATGY 4 cut(s) 139, 380, 1652, 1673
XcmI CCANNNNNNNNNTGG 3 cut(s) 270, 808, 1536
XspI CTAG 4 cut(s) 59, 402, 855, 1155
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.