RLG00000013595

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001075
Physical Location & Seq
Forward (+)
33811809 .. 33815242
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013595

Sequence Viewer

Length: 1761 bp
ATGGGTGTGCTCCAACAGTTGCTGGGTTCTCTGCTTCTATGCACTGCGCTTCTTTGCCTAGTTGAAGGAAATGTTCACTACTATGACTTTGTACTCAAGGAGGCCAACTTTACAAGGTTATGTCAGACTAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGCCATTCGTGTGCAAAAAGGTGATACTGTTTATGTCAATGTCCACAATCAAGGAGACTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCAAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACACAGTGTCCAATTGAACCCGGATCAAATTTCACTTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCATGCTCACAGTGATTGGACCAGAGCTAGTGTCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACTGGTTTTCCATTTCCAGAGCCAGATGGAGAAGAAATAGTTGTACTTGGTTCTTGGTATTATATTGATAATCTGAATGAGGTGGTTGCTGAGGACGTCGAAGAAGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTTGGCCTATGCTCAAATGATAGTACATATCGCTGGCAAGTGGAGTATGGCAAGACATACCTTCTTCGCCTGGTTAGTGCAGTTATGAATGCAGAACTTTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTTAAACCAATTGTGACCGATAATATCATGATTTCCCCCGGACAAACAATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGCGTTAGGCCAGATGATCAAGAATATGACAAGTCAAATGTTACAGCAATTCTGGAGTATAAAGGCAATTACACAACCCCAGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCAATAGAATTTACAGACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGACGATGTGACAGTTGCTCTTCTTGCAAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGAAAATTTGCCTGATAATACTGAGGTAACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTAGAAATAGTGTTTCAGGGTACTAATACGATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGCTATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGATAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTGTTTCAGTCCCAATGAAAGGATGGCTTGCTATCAGGTTCAAAGCAAGTAATCCTGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGATGCACACCGGAGACGAGCATGCGAGAACCTCCTCCATACATGCCTCCCTGCACTGATTCACCTACCATCAGGTTGCGACCTTCCCACAAATTCAGCAGGACGGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

587

Amino Acids

65.87

Weight (kDa)

4.79

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 146 5.7e-41 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 9.6e-36 Multicopper oxidase
Cu-oxidase_2 PF07731 424 - 549 6.4e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 1006
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 1091
AclWI GGATC 5 cut(s) 328, 1450, 1469, 1493, 1608
AcsI RAATTY 5 cut(s) 325, 1007, 1285, 1456, 1739
AcyI GRCGYC 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 7 cut(s) 93, 486, 625, 970, 1085, 1343, 1375
AfiI CCNNNNNNNGG 2 cut(s) 1212, 1535
AgsI TTSAA 3 cut(s) 65, 314, 1558
AjnI CCWGG 4 cut(s) 160, 592, 669, 1570
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 7 cut(s) 401, 821, 1029, 1173, 1428, 1485, 1626
AluI AGCT 7 cut(s) 401, 821, 1029, 1173, 1428, 1485, 1626
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 4 cut(s) 216, 434, 834, 1655
AlwI GGATC 5 cut(s) 328, 1450, 1469, 1493, 1608
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 5 cut(s) 102, 164, 229, 604, 875
ApeKI GCWGC 1 cut(s) 851
ApoI RAATTY 5 cut(s) 325, 1007, 1285, 1456, 1739
AseI ATTAAT 1 cut(s) 1386
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 164, 284, 393
AsuC2I CCSGG 2 cut(s) 318, 792
AsuHPI GGTGA 3 cut(s) 200, 1075, 1701
AvaII GGWCC 2 cut(s) 284, 393
BanI GGYRCC 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 961, 993
Bbv12I GWGCWC 1 cut(s) 12
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 838
BccI CCATC 5 cut(s) 275, 461, 737, 1533, 1724
BciT130I CCWGG 4 cut(s) 162, 594, 671, 1572
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 2 cut(s) 318, 792
BcoDI GTCTC 4 cut(s) 216, 434, 834, 1655
BfaI CTAG 3 cut(s) 59, 402, 855
BfmI CTRYAG 3 cut(s) 1188, 1210, 1423
BfuAI ACCTGC 1 cut(s) 1006
BisI GCNGC 1 cut(s) 852
BlpI GCTNAGC 1 cut(s) 1030
BlsI GCNGC 1 cut(s) 853
Bme1390I CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
Bme18I GGWCC 2 cut(s) 284, 393
BmgT120I GGNCC 3 cut(s) 164, 284, 393
BmiI GGNNCC 2 cut(s) 269, 415
BmrFI CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
BmsI GCATC 3 cut(s) 265, 1400, 1640
BpmI CTGGAG 1 cut(s) 941
Bpu10I CCTNAGC 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 1030
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 318, 792
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 1323, 1571
BsaWI WCCGGW 1 cut(s) 1657
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 2 cut(s) 1212, 1535
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 3 cut(s) 248, 448, 826
BseBI CCWGG 4 cut(s) 162, 594, 671, 1572
BseDI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 1323, 1571
BseGI GGATG 4 cut(s) 748, 1402, 1544, 1655
BseLI CCNNNNNNNGG 2 cut(s) 1212, 1535
BseMII CTCAG 4 cut(s) 279, 522, 1251, 1293
BseNI ACTGG 3 cut(s) 248, 448, 826
BseRI GAGGAG 2 cut(s) 549, 1671
BseXI GCAGC 1 cut(s) 838
BseYI CCCAGC 3 cut(s) 22, 946, 1173
BsgI GTGCAG 2 cut(s) 699, 1684
BshFI GGCC 5 cut(s) 104, 166, 231, 606, 877
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 4 cut(s) 318, 792, 1061, 1658
BslFI GGGAC 1 cut(s) 1511
BslI CCNNNNNNNGG 2 cut(s) 1212, 1535
BsmAI GTCTC 4 cut(s) 216, 434, 834, 1655
BsmBI CGTCTC 1 cut(s) 1655
BsmFI GGGAC 1 cut(s) 1511
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 5 cut(s) 104, 166, 231, 606, 877
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1407I TGTACA 1 cut(s) 1083
Bsp143I GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
Bsp1720I GCTNAGC 1 cut(s) 1030
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 1 cut(s) 1091
BspANI GGCC 5 cut(s) 104, 166, 231, 606, 877
BspCNI CTCAG 4 cut(s) 280, 523, 1250, 1294
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 2 cut(s) 269, 415
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 5 cut(s) 328, 1450, 1469, 1493, 1608
BspQI GCTCTTC 1 cut(s) 1152
BspT107I GGYRCC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 1060
BsrGI TGTACA 1 cut(s) 1083
BsrI ACTGG 3 cut(s) 248, 448, 826
BssAI RCCGGY 1 cut(s) 1060
BssECI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 1323, 1571
BssMI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
BssNI GRCGYC 1 cut(s) 537
BssT1I CCWWGG 4 cut(s) 263, 272, 409, 1323
Bst2UI CCWGG 4 cut(s) 162, 594, 671, 1572
Bst6I CTCTTC 2 cut(s) 1152, 1344
BstACI GRCGYC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 1083
BstC8I GCNNGC 7 cut(s) 137, 168, 378, 635, 1036, 1545, 1670
BstDEI CTNAG 6 cut(s) 129, 288, 531, 1030, 1237, 1302
BstDSI CCRYGG 1 cut(s) 409
BstENI CCTNNNNNAGG 1 cut(s) 1210
BstF5I GGATG 4 cut(s) 748, 1402, 1544, 1655
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 6 cut(s) 323, 886, 1445, 1477, 1501, 1603
BstMAI GTCTC 4 cut(s) 216, 434, 834, 1655
BstMBI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
BstMWI GCNNNNNNNGC 4 cut(s) 253, 570, 1035, 1151
BstNI CCWGG 4 cut(s) 162, 594, 671, 1572
BstNSI RCATGY 4 cut(s) 139, 380, 1672, 1693
BstSCI CCNGG 6 cut(s) 160, 316, 592, 669, 790, 1570
BstSFI CTRYAG 3 cut(s) 1188, 1210, 1423
BstV1I GCAGC 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 1474
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 1474
BsuRI GGCC 5 cut(s) 104, 166, 231, 606, 877
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 4 cut(s) 748, 1402, 1544, 1655
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 8 cut(s) 42, 241, 308, 391, 726, 1515, 1588, 1701
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 7 cut(s) 137, 168, 378, 635, 1036, 1545, 1670
CaiI CAGNNNCTG 1 cut(s) 22
CciI TCATGA 1 cut(s) 780
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 1060
Cfr13I GGNCC 3 cut(s) 164, 284, 393
Csp6I GTAC 7 cut(s) 92, 485, 624, 969, 1084, 1342, 1374
CviQI GTAC 7 cut(s) 92, 485, 624, 969, 1084, 1342, 1374
DdeI CTNAG 6 cut(s) 129, 288, 531, 1030, 1237, 1302
DpnI GATC 6 cut(s) 322, 885, 1444, 1476, 1500, 1602
DpnII GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
DraI TTTAAA 1 cut(s) 1161
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 1152, 1344
EarI CTCTTC 2 cut(s) 1152, 1344
Eco130I CCWWGG 4 cut(s) 263, 272, 409, 1323
Eco47I GGWCC 2 cut(s) 284, 393
EcoNI CCTNNNNNAGG 1 cut(s) 1210
EcoRII CCWGG 4 cut(s) 160, 592, 669, 1570
EcoT14I CCWWGG 4 cut(s) 263, 272, 409, 1323
EcoT22I ATGCAT 1 cut(s) 1393
ErhI CCWWGG 4 cut(s) 263, 272, 409, 1323
Esp3I CGTCTC 1 cut(s) 1655
FalI AAGNNNNNCTT 6 cut(s) 256, 288, 351, 383, 1527, 1559
FaqI GGGAC 1 cut(s) 1511
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 1 cut(s) 852
FokI GGATG 4 cut(s) 755, 1389, 1551, 1662
Fsp4HI GCNGC 1 cut(s) 852
FspBI CTAG 3 cut(s) 59, 402, 855
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 852
GsaI CCCAGC 3 cut(s) 26, 950, 1177
GsuI CTGGAG 1 cut(s) 941
HaeIII GGCC 5 cut(s) 104, 166, 231, 606, 877
HapII CCGG 4 cut(s) 318, 792, 1061, 1658
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 537
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 2 cut(s) 1399, 1706
HpaII CCGG 4 cut(s) 318, 792, 1061, 1658
HphI GGTGA 3 cut(s) 200, 1075, 1701
Hpy166II GTNNAC 2 cut(s) 76, 211
Hpy188III TCNNGA 6 cut(s) 287, 458, 781, 887, 920, 1024
Hpy8I GTNNAC 2 cut(s) 76, 211
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 6 cut(s) 59, 353, 539, 671, 987, 1740
HpyCH4IV ACGT 1 cut(s) 537
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 570, 1035, 1151
HpyF3I CTNAG 6 cut(s) 129, 288, 531, 1030, 1237, 1302
HpySE526I ACGT 1 cut(s) 537
Hsp92I GRCGYC 1 cut(s) 537
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 2 cut(s) 15, 746
Lsp1109I GCAGC 1 cut(s) 838
LweI GCATC 3 cut(s) 265, 1400, 1640
MaeI CTAG 3 cut(s) 59, 402, 855
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 6 cut(s) 295, 766, 814, 907, 1135, 1306
MalI GATC 6 cut(s) 322, 885, 1444, 1476, 1500, 1602
MboI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
MboII GAAGA 7 cut(s) 485, 554, 656, 1139, 1361, 1519, 1654
MfeI CAATTG 2 cut(s) 309, 762
MflI RGATCY 1 cut(s) 1474
MhlI GDGCHC 1 cut(s) 12
MmeI TCCRAC 3 cut(s) 37, 1208, 1420
Mph1103I ATGCAT 1 cut(s) 1393
MseI TTAA 4 cut(s) 756, 1160, 1179, 1386
MslI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
MspI CCGG 4 cut(s) 318, 792, 1061, 1658
MspR9I CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
MssI GTTTAAAC 1 cut(s) 1161
MunI CAATTG 2 cut(s) 309, 762
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 4 cut(s) 162, 594, 671, 1572
MwoI GCNNNNNNNGC 4 cut(s) 253, 570, 1035, 1151
NciI CCSGG 2 cut(s) 318, 792
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 751
NdeII GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
NlaIV GGNNCC 2 cut(s) 269, 415
NmuCI GTSAC 4 cut(s) 295, 766, 814, 1135
NsiI ATGCAT 1 cut(s) 1393
NspI RCATGY 4 cut(s) 139, 380, 1672, 1693
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 3 cut(s) 139, 380, 1672
PagI TCATGA 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 1152
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 2 cut(s) 1399, 1706
PkrI GCNGC 1 cut(s) 853
PmeI GTTTAAAC 1 cut(s) 1161
PshBI ATTAAT 1 cut(s) 1386
Psp6I CCWGG 4 cut(s) 160, 592, 669, 1570
PspFI CCCAGC 3 cut(s) 22, 946, 1173
PspGI CCWGG 4 cut(s) 160, 592, 669, 1570
PspN4I GGNNCC 2 cut(s) 269, 415
PspPI GGNCC 3 cut(s) 164, 284, 393
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 1 cut(s) 1474
RsaI GTAC 7 cut(s) 93, 486, 625, 970, 1085, 1343, 1375
RsaNI GTAC 7 cut(s) 92, 485, 624, 969, 1084, 1342, 1374
RseI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 4 cut(s) 756, 1160, 1179, 1386
SatI GCNGC 1 cut(s) 852
Sau3AI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
Sau96I GGNCC 3 cut(s) 164, 284, 393
ScrFI CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 3 cut(s) 265, 1400, 1640
SfcI CTRYAG 3 cut(s) 1188, 1210, 1423
SinI GGWCC 2 cut(s) 284, 393
SmiMI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SphI GCATGC 3 cut(s) 139, 380, 1672
SsiI CCGC 1 cut(s) 1091
SspMI CTAG 3 cut(s) 59, 402, 855
StyD4I CCNGG 6 cut(s) 160, 316, 592, 669, 790, 1570
StyI CCWWGG 4 cut(s) 263, 272, 409, 1323
TaiI ACGT 1 cut(s) 540
TaqI TCGA 2 cut(s) 540, 1603
TaqII GACCGA 1 cut(s) 785
TatI WGTACW 5 cut(s) 91, 484, 623, 1083, 1341
TfiI GAWTC 2 cut(s) 1399, 1706
Tru1I TTAA 4 cut(s) 756, 1160, 1179, 1386
Tru9I TTAA 4 cut(s) 756, 1160, 1179, 1386
TscAI CASTG 8 cut(s) 49, 248, 308, 391, 733, 1522, 1595, 1708
TseFI GTSAC 4 cut(s) 295, 766, 814, 1135
TseI GCWGC 1 cut(s) 851
Tsp45I GTSAC 4 cut(s) 295, 766, 814, 1135
TspDTI ATGAA 6 cut(s) 596, 701, 1115, 1296, 1362, 1547
TspRI CASTG 8 cut(s) 49, 248, 308, 391, 733, 1522, 1595, 1708
VpaK11BI GGWCC 2 cut(s) 284, 393
VspI ATTAAT 1 cut(s) 1386
XagI CCTNNNNNAGG 1 cut(s) 1210
XapI RAATTY 5 cut(s) 325, 1007, 1285, 1456, 1739
XceI RCATGY 4 cut(s) 139, 380, 1672, 1693
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 1536
XspI CTAG 3 cut(s) 59, 402, 855
ZraI GACGTC 1 cut(s) 538
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.