Rroxscaffold_7G00194600

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001075
Physical Location & Seq
Reverse (-)
36545115 .. 36557075
11961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00194600.1

Sequence Viewer

Length: 1338 bp
ATGCCCGTTGATAATACAGAGTTTCCATTTCCTAAACCTGATGGAGAAGATATTATTGTATTTGGAAGTTGGTATAATACTACTGAAGATGTGAACGATGTGGTTGCTGAGGACCTCCAAGATGGTAGTGACACTCCTAACTCGGATTGCTACACAATAAATGGACAACCAGGAGATTTTCTCCCATGTTCAGATGCTAGTACGTATCGTTTGCAAGTGGAGTATGGCAAGACATATCTTCTTCGCCTTATTAATGCAGTTATGAATGTAGAAATTTATTTTGCAATTGCTGAACATAGCCTCACTGTTGTCGGTATGGATGCATCATATGTTAAACCTATTGTGACAGACTTTATCATGATTGGTCCTGGACAAACAATGGATATTTTGATCACGGCCAACCAGTCTCAGGGCACATACTACATGGCCGGTAGACAATATAACACTGTGAGGAAGGATATTTTAGACTATGATAATTCAACTGTGACAGGAATTCTCGAGTATAAAGGCAATTACACACTCCCAACAAACTCAATTTTTCCAAGCACCCTACCTGCCATGTTTGATTTAGTAGCAGCAAGAAAATTCACAGATCGTCTCAAGAGCCTGGCAACTATAAGCCACCCAGCAGATGTGCCGAAGAATTTAACCACCAGAATGTACTTAATAGCTTCATCTAATTATGGTTTCGTTAATCACTCTGGTCAGTACGAAACTGGCGTAGCTGCTAGTTTAAACAATGTAAGCTGGGCTAACCCATCAACAGATGTGTTACGTGCATACTACAGGAACATCGAAGGATTTTATACCTCAGACTTTCCTAATTTTCCACCATCATTTTATGACTTCGCGTCAGAAGATTATCCTGATACTACGATCATAACTGCCAAAGCAACCAAGGTCAAGGTATTAGAGTACAATGAAGAGGTTGAAATAGTGTTCCAAGGTACTAATGTGCTTGATGCTTCTGAGGATCATCCCATGCACATGCATGGCTATAGCTTTTATGTAGTTGGATCAGGTACTGGAAATTTTGACAATGAAACAGACCCCAAAGCGTATAATTTGGTTGATCCTCCAAAGATGAATACTGTTTCACTCCCAAAAGTTGGATGGGTTGCTATTAGATTCAAAGCAAGTAATCCTGGGGTTTGGTTTTGGCATTGTCATTTTGATCGACATTTGACTTGGGGTATGAGCTTTGTGATGATAGTCAAAGATGGAGGTACACCTGAGACCAGCATACGTAAACCACCACCAAACATGCCTCCATGTAATGGTTCACCAACCATCAGGCTACAACCTTTTGATATGCATGCTCAGCAAGATAGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

445

Amino Acids

49.78

Weight (kDa)

4.76

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 18 - 171 8.4e-36 Multicopper oxidase
Cu-oxidase_2 PF07731 292 - 407 1.3e-39 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 2 cut(s) 1109, 1277
AccI GTMKAC 1 cut(s) 433
AccII CGCG 1 cut(s) 851
AclWI GGATC 3 cut(s) 981, 1024, 1067
AcoI YGGCCR 2 cut(s) 396, 426
AcsI RAATTY 5 cut(s) 273, 492, 584, 643, 1030
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 7 cut(s) 202, 662, 710, 917, 949, 1024, 1228
AfiI CCNNNNNNNGG 3 cut(s) 409, 1109, 1277
AgsI TTSAA 3 cut(s) 480, 932, 1132
AhdI GACNNNNNGTC 1 cut(s) 850
AjnI CCWGG 4 cut(s) 169, 367, 606, 1144
AluBI AGCT 5 cut(s) 671, 725, 747, 1002, 1200
AluI AGCT 5 cut(s) 671, 725, 747, 1002, 1200
Alw26I GTCTC 3 cut(s) 411, 602, 1229
AlwI GGATC 3 cut(s) 981, 1024, 1067
AlwNI CAGNNNCTG 1 cut(s) 1025
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 2 cut(s) 396, 426
ApeKI GCWGC 2 cut(s) 575, 725
ApoI RAATTY 5 cut(s) 273, 492, 584, 643, 1030
AseI ATTAAT 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 112, 365
AsuHPI GGTGA 1 cut(s) 1275
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 2 cut(s) 112, 365
BaeGI GKGCMC 1 cut(s) 416
BaeI ACNNNNGTAYC 2 cut(s) 939, 972
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 587, 712
BccI CCATC 7 cut(s) 35, 116, 766, 841, 1107, 1214, 1298
BceAI ACGGC 1 cut(s) 411
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 4 cut(s) 171, 369, 608, 1146
BclI TGATCA 1 cut(s) 390
BcoDI GTCTC 3 cut(s) 411, 602, 1229
BfaI CTAG 2 cut(s) 198, 729
BfmI CTRYAG 2 cut(s) 784, 997
BfuAI ACCTGC 1 cut(s) 562
BisI GCNGC 2 cut(s) 576, 726
BlpI GCTNAGC 1 cut(s) 1320
BlsI GCNGC 2 cut(s) 577, 727
Bme1390I CCNGG 4 cut(s) 171, 369, 608, 1146
Bme18I GGWCC 2 cut(s) 112, 365
BmeRI GACNNNNNGTC 1 cut(s) 850
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 2 cut(s) 112, 365
BmrFI CCNGG 4 cut(s) 171, 369, 608, 1146
BmsI GCATC 4 cut(s) 184, 310, 332, 952
BplI GAGNNNNNCTC 2 cut(s) 165, 197
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 1320
BpuEI CTTGAG 1 cut(s) 584
BsaAI YACGTR 3 cut(s) 204, 776, 1247
BsaI GGTCTC 1 cut(s) 1229
BsaJI CCNNGG 3 cut(s) 897, 943, 1145
Bsc4I CCNNNNNNNGG 3 cut(s) 409, 1109, 1277
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 3 cut(s) 403, 721, 1030
BseBI CCWGG 4 cut(s) 171, 369, 608, 1146
BseDI CCNNGG 3 cut(s) 897, 943, 1145
BseGI GGATG 3 cut(s) 325, 976, 1118
BseLI CCNNNNNNNGG 3 cut(s) 409, 1109, 1277
BseMII CTCAG 6 cut(s) 99, 422, 825, 960, 1224, 1334
BseNI ACTGG 3 cut(s) 403, 721, 1030
BseSI GKGCMC 1 cut(s) 416
BseXI GCAGC 2 cut(s) 587, 712
BseYI CCCAGC 2 cut(s) 625, 747
Bsh1236I CGCG 1 cut(s) 851
BshFI GGCC 2 cut(s) 398, 428
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 1 cut(s) 429
BslI CCNNNNNNNGG 3 cut(s) 409, 1109, 1277
BsmAI GTCTC 3 cut(s) 411, 602, 1229
BsmBI CGTCTC 1 cut(s) 602
BsnI GGCC 2 cut(s) 398, 428
Bso31I GGTCTC 1 cut(s) 1229
BsoBI CYCGRG 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
Bsp1720I GCTNAGC 1 cut(s) 1320
BspANI GGCC 2 cut(s) 398, 428
BspCNI CTCAG 6 cut(s) 100, 421, 824, 961, 1225, 1333
BspFNI CGCG 1 cut(s) 851
BspHI TCATGA 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 3 cut(s) 981, 1024, 1067
BspTNI GGTCTC 1 cut(s) 1229
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 3 cut(s) 403, 721, 1030
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 3 cut(s) 897, 943, 1145
BssMI GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
BssT1I CCWWGG 2 cut(s) 897, 943
Bst2UI CCWGG 4 cut(s) 171, 369, 608, 1146
Bst4CI ACNGT 4 cut(s) 307, 448, 484, 1093
Bst6I CTCTTC 1 cut(s) 918
BstBAI YACGTR 3 cut(s) 204, 776, 1247
BstC8I GCNNGC 1 cut(s) 1317
BstDEI CTNAG 6 cut(s) 108, 408, 811, 969, 1233, 1320
BstF5I GGATG 3 cut(s) 325, 976, 1118
BstFNI CGCG 1 cut(s) 851
BstKTI GATC 7 cut(s) 393, 595, 879, 976, 1019, 1075, 1177
BstMAI GTCTC 3 cut(s) 411, 602, 1229
BstMBI GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
BstMWI GCNNNNNNNGC 1 cut(s) 1321
BstNI CCWGG 4 cut(s) 171, 369, 608, 1146
BstNSI RCATGY 3 cut(s) 991, 1267, 1319
BstSCI CCNGG 4 cut(s) 169, 367, 606, 1144
BstSFI CTRYAG 2 cut(s) 784, 997
BstSLI GKGCMC 1 cut(s) 416
BstSNI TACGTA 2 cut(s) 204, 1247
BstUI CGCG 1 cut(s) 851
BstV1I GCAGC 2 cut(s) 587, 712
BsuRI GGCC 2 cut(s) 398, 428
BtsCI GGATG 3 cut(s) 325, 976, 1118
BtsIMutI CAGTG 2 cut(s) 303, 444
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 1317
CaiI CAGNNNCTG 1 cut(s) 1025
CciI TCATGA 1 cut(s) 357
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 2 cut(s) 112, 365
CseI GACGC 1 cut(s) 840
Csp6I GTAC 7 cut(s) 201, 661, 709, 916, 948, 1023, 1227
CviQI GTAC 7 cut(s) 201, 661, 709, 916, 948, 1023, 1227
DdeI CTNAG 6 cut(s) 108, 408, 811, 969, 1233, 1320
DpnI GATC 7 cut(s) 392, 594, 878, 975, 1018, 1074, 1176
DpnII GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
DraI TTTAAA 1 cut(s) 735
DriI GACNNNNNGTC 1 cut(s) 850
EaeI YGGCCR 2 cut(s) 396, 426
Eam1104I CTCTTC 1 cut(s) 918
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 1 cut(s) 918
Eco105I TACGTA 2 cut(s) 204, 1247
Eco130I CCWWGG 2 cut(s) 897, 943
Eco31I GGTCTC 1 cut(s) 1229
Eco47I GGWCC 2 cut(s) 112, 365
Eco57I CTGAAG 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 4 cut(s) 169, 367, 606, 1144
EcoT14I CCWWGG 2 cut(s) 897, 943
EcoT22I ATGCAT 3 cut(s) 325, 993, 1317
ErhI CCWWGG 2 cut(s) 897, 943
Esp3I CGTCTC 1 cut(s) 602
FauNDI CATATG 1 cut(s) 328
FbaI TGATCA 1 cut(s) 390
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 2 cut(s) 576, 726
FokI GGATG 3 cut(s) 332, 963, 1125
Fsp4HI GCNGC 2 cut(s) 576, 726
FspBI CTAG 2 cut(s) 198, 729
GluI GCNGC 2 cut(s) 576, 726
GsaI CCCAGC 2 cut(s) 629, 751
HaeIII GGCC 2 cut(s) 398, 428
HapII CCGG 1 cut(s) 429
HgaI GACGC 1 cut(s) 840
HinfI GANTC 1 cut(s) 1128
HpaII CCGG 1 cut(s) 429
HphI GGTGA 1 cut(s) 1275
Hpy166II GTNNAC 5 cut(s) 94, 434, 1229, 1250, 1283
Hpy188I TCNGA 5 cut(s) 145, 193, 814, 856, 970
Hpy188III TCNNGA 4 cut(s) 358, 497, 601, 866
Hpy8I GTNNAC 5 cut(s) 94, 434, 1229, 1250, 1283
HpyAV CCTTC 2 cut(s) 448, 791
HpyCH4III ACNGT 4 cut(s) 307, 448, 484, 1093
HpyCH4IV ACGT 3 cut(s) 203, 775, 1246
HpyCH4V TGCA 8 cut(s) 214, 257, 284, 323, 779, 985, 991, 1315
HpyF10VI GCNNNNNNNGC 1 cut(s) 1321
HpyF3I CTNAG 6 cut(s) 108, 408, 811, 969, 1233, 1320
HpySE526I ACGT 3 cut(s) 203, 775, 1246
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
Lsp1109I GCAGC 2 cut(s) 587, 712
LweI GCATC 4 cut(s) 184, 310, 332, 952
MaeI CTAG 2 cut(s) 198, 729
MaeII ACGT 3 cut(s) 203, 775, 1246
MaeIII GTNAC 4 cut(s) 128, 343, 484, 771
MalI GATC 7 cut(s) 392, 594, 878, 975, 1018, 1074, 1176
MboI GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
MboII GAAGA 7 cut(s) 59, 98, 230, 233, 652, 869, 935
MfeI CAATTG 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 994, 1090
Mph1103I ATGCAT 3 cut(s) 325, 993, 1317
MseI TTAA 6 cut(s) 252, 333, 647, 665, 693, 734
MslI CAYNNNNRTG 3 cut(s) 656, 986, 990
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 4 cut(s) 171, 369, 608, 1146
MssI GTTTAAAC 1 cut(s) 735
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 4 cut(s) 171, 369, 608, 1146
MvnI CGCG 1 cut(s) 851
MwoI GCNNNNNNNGC 1 cut(s) 1321
NdeI CATATG 1 cut(s) 328
NdeII GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
NmuCI GTSAC 3 cut(s) 128, 343, 484
NsiI ATGCAT 3 cut(s) 325, 993, 1317
NspI RCATGY 3 cut(s) 991, 1267, 1319
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 1 cut(s) 497
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PfeI GAWTC 1 cut(s) 1128
PflMI CCANNNNNTGG 2 cut(s) 1109, 1277
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 2 cut(s) 577, 727
PmeI GTTTAAAC 1 cut(s) 735
Ppu21I YACGTR 3 cut(s) 204, 776, 1247
PpuMI RGGWCCY 1 cut(s) 112
PshBI ATTAAT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 4 cut(s) 169, 367, 606, 1144
PspFI CCCAGC 2 cut(s) 625, 747
PspGI CCWGG 4 cut(s) 169, 367, 606, 1144
PspPI GGNCC 2 cut(s) 112, 365
PspPPI RGGWCCY 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 1025
RsaI GTAC 7 cut(s) 202, 662, 710, 917, 949, 1024, 1228
RsaNI GTAC 7 cut(s) 201, 661, 709, 916, 948, 1023, 1227
RseI CAYNNNNRTG 3 cut(s) 656, 986, 990
SaqAI TTAA 6 cut(s) 252, 333, 647, 665, 693, 734
SatI GCNGC 2 cut(s) 576, 726
Sau3AI GATC 7 cut(s) 390, 592, 876, 973, 1016, 1072, 1174
Sau96I GGNCC 2 cut(s) 112, 365
ScrFI CCNGG 4 cut(s) 171, 369, 608, 1146
SduI GDGCHC 1 cut(s) 416
SfaNI GCATC 4 cut(s) 184, 310, 332, 952
SfcI CTRYAG 2 cut(s) 784, 997
Sfr274I CTCGAG 1 cut(s) 497
SinI GGWCC 2 cut(s) 112, 365
SlaI CTCGAG 1 cut(s) 497
SmiMI CAYNNNNRTG 3 cut(s) 656, 986, 990
SmlI CTYRAG 2 cut(s) 497, 599
SmoI CTYRAG 2 cut(s) 497, 599
SnaBI TACGTA 2 cut(s) 204, 1247
SphI GCATGC 1 cut(s) 1319
SspMI CTAG 2 cut(s) 198, 729
StyD4I CCNGG 4 cut(s) 169, 367, 606, 1144
StyI CCWWGG 2 cut(s) 897, 943
TaaI ACNGT 4 cut(s) 307, 448, 484, 1093
TaiI ACGT 3 cut(s) 206, 778, 1249
TaqI TCGA 3 cut(s) 498, 795, 1177
TatI WGTACW 2 cut(s) 660, 915
TfiI GAWTC 1 cut(s) 1128
Tru1I TTAA 6 cut(s) 252, 333, 647, 665, 693, 734
Tru9I TTAA 6 cut(s) 252, 333, 647, 665, 693, 734
TscAI CASTG 2 cut(s) 310, 451
TseFI GTSAC 3 cut(s) 128, 343, 484
TseI GCWGC 2 cut(s) 575, 725
Tsp45I GTSAC 3 cut(s) 128, 343, 484
TspDTI ATGAA 5 cut(s) 278, 663, 936, 1056, 1100
TspRI CASTG 2 cut(s) 310, 451
Van91I CCANNNNNTGG 2 cut(s) 1109, 1277
VpaK11BI GGWCC 2 cut(s) 112, 365
VspI ATTAAT 1 cut(s) 252
XapI RAATTY 5 cut(s) 273, 492, 584, 643, 1030
XceI RCATGY 3 cut(s) 991, 1267, 1319
XcmI CCANNNNNNNNNTGG 1 cut(s) 1110
XhoI CTCGAG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 433
XspI CTAG 2 cut(s) 198, 729
Zsp2I ATGCAT 3 cut(s) 325, 993, 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.