Rw6G016990

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001075
Physical Location & Seq
Forward (+)
33635295 .. 33656529
21235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016990.1

Sequence Viewer

Length: 1770 bp
ATGGGTACCTTTCCAGATTTTCTGGGTTTGTTTCTGCTATGTGGGATGCTGCATTGCATTGTTGAATGCCATGTCCATTACCACGACTTTGTACTCAAGAAGGAGAACTTTACAAGATTATGTAACACAAAGAGCATGCTGGTAGTGAATGGCAGTTTTCCGGGGCCAGTCATTCGTGTGCAAAAGGGGGATACTGTCTTTGTCAATGTTCACAATCAAGGAGATTATGGACTTACTATTCACTGGCACGGAGTACATCAACCAAGAAATCCATGGTCAGATGGTCCTGAGTATATCACACAATGTGCCATTGAACCTGGTTCAAATTTTACTTACGAGGTTATATTTTCCGATGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGAATGGACCAGAGCAAGTGTCCATGGTGCCATTGTCATTATGCCCGTTGATAATACAGAGTTTCCATTTCCTAAACCTGATGGAGAAGATATTATTGTATTTGGAAGTTGGTATAATACTACTGAAGATGTGAACGATGTGGTTGCTGAGGATCTCCAAGATGGTAGTGACACTCCTAACTCGGATTGCTACACAATAAATGGACAACCAGGAGATTTTCTCCCATGTTCAAATGCTAGTACGTATCGTTTGCAAGTGGAGTATGGCAAGACATATCTTCTTCGCCTTATTAATGCAGTTATGAATGTAGAAATTTATTTTGCAATTGCTGAACATAGCCTCACTGTTGTCGGTATGGATGCATCATATGTTAAACCTATTGTGACAGACTTTATCATGATTGGTCCTGGACAAACAATGGATATTTTGATCACGGCCAACCAGCCTCAGGGCACATACTACATGGCCGGTAGACAATATAACACTGTGAGGAAGGATATTTTAGACTATGATAATTCAACCGTGACAGGAATTCTCGAGTATAAAGGCAATTATACACTCCCAACACACCCAATTTTTCCAAGTACCCTACCTACCTTATTTGATTTAGTAGCAGCAAAAAAATTCACAGATCGTCTGAAGAGCCTGGCAACCATAAGCCACCCAGCAGATGTGCCGAAGAATTTAACCACCAGAATGTACTTAATAGCTTCATCTAATTATGGTTTCGTTAATCACTCTGGTCAGTACGAAACTGGCGTAGCTGCTAGTTTAAACAATGTAAGCTGGGCTAACCCATCAACAGATGTGTTACGTGCATACTACAGGAACATGGAAGGATTTTATACCTCAGACTTTCCTAATTTTCCACCATCATTTTATGACTTCGCGTCAGAAGATTATCCTGATACTACGATCATAACTGCCAAAGCAACCAAGGTCAAGGTATTAGAGTACAATGAAGAGGTTGAAATAGTGTTCCAAGGTACTAATGTGCTTGATGCTTCTGAGGATCATCCCATGCACATGCATGGCTATAGCTTTTATGTAGTTGGATCAGGTACTGGAAATTTTGACAATGAAACAGACCACAAAGCGTATAATTTGGTTGATCCTCCAAAGATGAATACTGTTTCACTCCCAAAAGTAGGATGGGTTGCTATTAGATTCAAAGCAAGTAATCCTGGGGTTTGGTTTTGGCATTGTCATTTTGATCGACATTTGACTTGGGGTATGAGCTTTGTGATGATAGTCAAAGATGGAGGTACACCTGAGACGAGCATACGTAAACCACCCCCAAACATGCCTCCATGTAATGGTTCACCAACCATCAGGCTACAACCTTTTGATATGCTCAGCAAGATAGCAGGTGACACAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

589

Amino Acids

66.13

Weight (kDa)

5.22

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 144 3e-40 Multicopper oxidase
Cu-oxidase PF00394 160 - 313 5.7e-36 Multicopper oxidase
Cu-oxidase_2 PF07731 434 - 549 8.4e-39 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1745
Acc36I ACCTGC 1 cut(s) 1745
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 2 cut(s) 5, 413
AccB7I CCANNNNNTGG 1 cut(s) 1703
AccI GTMKAC 1 cut(s) 859
AccII CGCG 1 cut(s) 1277
AclWI GGATC 4 cut(s) 546, 1407, 1450, 1493
AcoI YGGCCR 2 cut(s) 822, 852
AcsI RAATTY 6 cut(s) 325, 699, 918, 1010, 1069, 1456
AcuI CTGAAG 2 cut(s) 531, 1046
AdeI CACNNNGTG 1 cut(s) 305
AfiI CCNNNNNNNGG 3 cut(s) 835, 1535, 1703
AgsI TTSAA 7 cut(s) 65, 314, 324, 618, 906, 1358, 1558
AhdI GACNNNNNGTC 1 cut(s) 1276
AjnI CCWGG 5 cut(s) 316, 595, 793, 1032, 1570
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 5 cut(s) 1097, 1151, 1173, 1428, 1626
AluI AGCT 5 cut(s) 1097, 1151, 1173, 1428, 1626
Alw26I GTCTC 1 cut(s) 1655
AlwI GGATC 4 cut(s) 546, 1407, 1450, 1493
AlwNI CAGNNNCTG 1 cut(s) 1451
Ama87I CYCGRG 1 cut(s) 923
AoxI GGCC 3 cut(s) 164, 822, 852
ApeKI GCWGC 3 cut(s) 49, 1001, 1151
ApoI RAATTY 6 cut(s) 325, 699, 918, 1010, 1069, 1456
AseI ATTAAT 1 cut(s) 678
Asp718I GGTACC 1 cut(s) 5
AspS9I GGNCC 4 cut(s) 164, 284, 393, 791
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 1701, 1769
AvaI CYCGRG 1 cut(s) 923
AvaII GGWCC 3 cut(s) 284, 393, 791
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 1 cut(s) 842
BaeI ACNNNNGTAYC 2 cut(s) 1365, 1398
BanI GGYRCC 2 cut(s) 5, 413
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 3 cut(s) 36, 1013, 1138
BccI CCATC 8 cut(s) 275, 461, 542, 1192, 1267, 1533, 1640, 1724
BceAI ACGGC 1 cut(s) 837
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 5 cut(s) 318, 597, 795, 1034, 1572
BciVI GTATCC 1 cut(s) 184
BclI TGATCA 1 cut(s) 816
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 1655
BfaI CTAG 2 cut(s) 624, 1155
BfmI CTRYAG 2 cut(s) 1210, 1423
BfuAI ACCTGC 1 cut(s) 1745
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 3 cut(s) 50, 1002, 1152
BlpI GCTNAGC 1 cut(s) 1742
BlsI GCNGC 3 cut(s) 51, 1003, 1153
Bme1390I CCNGG 6 cut(s) 162, 318, 597, 795, 1034, 1572
Bme18I GGWCC 3 cut(s) 284, 393, 791
BmeRI GACNNNNNGTC 1 cut(s) 1276
BmeT110I CYCGRG 1 cut(s) 923
BmgT120I GGNCC 4 cut(s) 164, 284, 393, 791
BmiI GGNNCC 3 cut(s) 7, 165, 415
BmrFI CCNGG 6 cut(s) 162, 318, 597, 795, 1034, 1572
BmsI GCATC 4 cut(s) 36, 736, 758, 1378
BplI GAGNNNNNCTC 2 cut(s) 591, 623
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 1742
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 162
BsaAI YACGTR 3 cut(s) 630, 1202, 1673
BsaJI CCNNGG 6 cut(s) 161, 272, 409, 1323, 1369, 1571
Bsc4I CCNNNNNNNGG 3 cut(s) 835, 1535, 1703
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 4 cut(s) 167, 248, 1147, 1456
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 1 cut(s) 52
BseBI CCWGG 5 cut(s) 318, 597, 795, 1034, 1572
BseDI CCNNGG 6 cut(s) 161, 272, 409, 1323, 1369, 1571
BseGI GGATG 4 cut(s) 51, 751, 1402, 1544
BseLI CCNNNNNNNGG 3 cut(s) 835, 1535, 1703
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 7 cut(s) 279, 525, 848, 1251, 1386, 1650, 1756
BseNI ACTGG 4 cut(s) 167, 248, 1147, 1456
BseSI GKGCMC 1 cut(s) 842
BseXI GCAGC 3 cut(s) 36, 1013, 1138
BseYI CCCAGC 2 cut(s) 1051, 1173
Bsh1236I CGCG 1 cut(s) 1277
BshFI GGCC 3 cut(s) 166, 824, 854
BshNI GGYRCC 2 cut(s) 5, 413
BsiHKCI CYCGRG 1 cut(s) 923
BsiSI CCGG 2 cut(s) 161, 855
BslI CCNNNNNNNGG 3 cut(s) 835, 1535, 1703
BsmAI GTCTC 1 cut(s) 1655
BsmBI CGTCTC 1 cut(s) 1655
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 3 cut(s) 166, 824, 854
BsoBI CYCGRG 1 cut(s) 923
Bsp1286I GDGCHC 1 cut(s) 842
Bsp143I GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
Bsp1720I GCTNAGC 1 cut(s) 1742
Bsp19I CCATGG 2 cut(s) 272, 409
BspANI GGCC 3 cut(s) 166, 824, 854
BspCNI CTCAG 7 cut(s) 280, 526, 847, 1250, 1387, 1651, 1755
BspFNI CGCG 1 cut(s) 1277
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 3 cut(s) 7, 165, 415
BspMI ACCTGC 1 cut(s) 1745
BspPI GGATC 4 cut(s) 546, 1407, 1450, 1493
BspQI GCTCTTC 1 cut(s) 1022
BspT107I GGYRCC 2 cut(s) 5, 413
BsrDI GCAATG 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 854
BsrI ACTGG 4 cut(s) 167, 248, 1147, 1456
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 6 cut(s) 161, 272, 409, 1323, 1369, 1571
BssMI GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
BssT1I CCWWGG 4 cut(s) 272, 409, 1323, 1369
Bst2UI CCWGG 5 cut(s) 318, 597, 795, 1034, 1572
Bst4CI ACNGT 5 cut(s) 196, 733, 874, 910, 1519
Bst6I CTCTTC 2 cut(s) 1022, 1344
BstBAI YACGTR 3 cut(s) 630, 1202, 1673
BstC8I GCNNGC 2 cut(s) 137, 378
BstDEI CTNAG 7 cut(s) 288, 534, 834, 1237, 1395, 1659, 1742
BstDSI CCRYGG 2 cut(s) 272, 409
BstF5I GGATG 4 cut(s) 51, 751, 1402, 1544
BstFNI CGCG 1 cut(s) 1277
BstKTI GATC 8 cut(s) 541, 819, 1021, 1305, 1402, 1445, 1501, 1603
BstMAI GTCTC 1 cut(s) 1655
BstMBI GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
BstNI CCWGG 5 cut(s) 318, 597, 795, 1034, 1572
BstNSI RCATGY 4 cut(s) 139, 380, 1417, 1693
BstSCI CCNGG 6 cut(s) 160, 316, 595, 793, 1032, 1570
BstSFI CTRYAG 2 cut(s) 1210, 1423
BstSLI GKGCMC 1 cut(s) 842
BstSNI TACGTA 2 cut(s) 630, 1673
BstUI CGCG 1 cut(s) 1277
BstV1I GCAGC 3 cut(s) 36, 1013, 1138
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 3 cut(s) 166, 824, 854
BtgI CCRYGG 2 cut(s) 272, 409
BtsCI GGATG 4 cut(s) 51, 751, 1402, 1544
BtsIMutI CAGTG 3 cut(s) 241, 729, 870
BveI ACCTGC 1 cut(s) 1745
Cac8I GCNNGC 2 cut(s) 137, 378
CaiI CAGNNNCTG 1 cut(s) 1451
CciI TCATGA 1 cut(s) 783
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 4 cut(s) 164, 284, 393, 791
CseI GACGC 1 cut(s) 1266
CsiI ACCWGGT 1 cut(s) 316
DdeI CTNAG 7 cut(s) 288, 534, 834, 1237, 1395, 1659, 1742
DpnI GATC 8 cut(s) 540, 818, 1020, 1304, 1401, 1444, 1500, 1602
DpnII GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
DraI TTTAAA 1 cut(s) 1161
DraIII CACNNNGTG 1 cut(s) 305
DriI GACNNNNNGTC 1 cut(s) 1276
EaeI YGGCCR 2 cut(s) 822, 852
Eam1104I CTCTTC 2 cut(s) 1022, 1344
Eam1105I GACNNNNNGTC 1 cut(s) 1276
EarI CTCTTC 2 cut(s) 1022, 1344
Eco105I TACGTA 2 cut(s) 630, 1673
Eco130I CCWWGG 4 cut(s) 272, 409, 1323, 1369
Eco47I GGWCC 3 cut(s) 284, 393, 791
Eco57I CTGAAG 2 cut(s) 531, 1046
Eco81I CCTNAGG 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 923
EcoRI GAATTC 1 cut(s) 918
EcoRII CCWGG 5 cut(s) 316, 595, 793, 1032, 1570
EcoT14I CCWWGG 4 cut(s) 272, 409, 1323, 1369
EcoT22I ATGCAT 2 cut(s) 751, 1419
ErhI CCWWGG 4 cut(s) 272, 409, 1323, 1369
Esp3I CGTCTC 1 cut(s) 1655
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 351, 383
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 1 cut(s) 816
FblI GTMKAC 1 cut(s) 859
Fnu4HI GCNGC 3 cut(s) 50, 1002, 1152
FokI GGATG 4 cut(s) 58, 758, 1389, 1551
Fsp4HI GCNGC 3 cut(s) 50, 1002, 1152
FspBI CTAG 2 cut(s) 624, 1155
GluI GCNGC 3 cut(s) 50, 1002, 1152
GsaI CCCAGC 2 cut(s) 1055, 1177
HaeIII GGCC 3 cut(s) 166, 824, 854
HapII CCGG 2 cut(s) 161, 855
HgaI GACGC 1 cut(s) 1266
HinfI GANTC 1 cut(s) 1554
HpaII CCGG 2 cut(s) 161, 855
HphI GGTGA 2 cut(s) 1701, 1769
Hpy166II GTNNAC 6 cut(s) 211, 520, 860, 1655, 1676, 1709
Hpy188I TCNGA 7 cut(s) 280, 352, 571, 1026, 1240, 1282, 1396
Hpy188III TCNNGA 6 cut(s) 14, 97, 287, 784, 923, 1292
Hpy8I GTNNAC 6 cut(s) 211, 520, 860, 1655, 1676, 1709
HpyAV CCTTC 4 cut(s) 94, 353, 874, 1217
HpyCH4III ACNGT 5 cut(s) 196, 733, 874, 910, 1519
HpyCH4IV ACGT 3 cut(s) 629, 1201, 1672
HpyF3I CTNAG 7 cut(s) 288, 534, 834, 1237, 1395, 1659, 1742
HpySE526I ACGT 3 cut(s) 629, 1201, 1672
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
LguI GCTCTTC 1 cut(s) 1022
Lsp1109I GCAGC 3 cut(s) 36, 1013, 1138
LweI GCATC 4 cut(s) 36, 736, 758, 1378
MabI ACCWGGT 1 cut(s) 316
MaeI CTAG 2 cut(s) 624, 1155
MaeII ACGT 3 cut(s) 629, 1201, 1672
MaeIII GTNAC 6 cut(s) 122, 554, 769, 910, 1197, 1757
MalI GATC 8 cut(s) 540, 818, 1020, 1304, 1401, 1444, 1500, 1602
MboI GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
MboII GAAGA 8 cut(s) 485, 524, 656, 659, 1039, 1078, 1295, 1361
MfeI CAATTG 1 cut(s) 711
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 1 cut(s) 842
MmeI TCCRAC 1 cut(s) 1420
Mph1103I ATGCAT 2 cut(s) 751, 1419
MseI TTAA 6 cut(s) 678, 759, 1073, 1091, 1119, 1160
MslI CAYNNNNRTG 5 cut(s) 176, 370, 1082, 1412, 1416
MspI CCGG 2 cut(s) 161, 855
MspR9I CCNGG 6 cut(s) 162, 318, 597, 795, 1034, 1572
MssI GTTTAAAC 1 cut(s) 1161
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 5 cut(s) 318, 597, 795, 1034, 1572
MvnI CGCG 1 cut(s) 1277
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 2 cut(s) 272, 409
NdeI CATATG 1 cut(s) 754
NdeII GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
NlaIV GGNNCC 3 cut(s) 7, 165, 415
NmuCI GTSAC 4 cut(s) 554, 769, 910, 1757
NsiI ATGCAT 2 cut(s) 751, 1419
NspI RCATGY 4 cut(s) 139, 380, 1417, 1693
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 2 cut(s) 139, 380
PaeR7I CTCGAG 1 cut(s) 923
PagI TCATGA 1 cut(s) 783
PaqCI CACCTGC 1 cut(s) 1745
PciSI GCTCTTC 1 cut(s) 1022
PcsI WCGNNNNNNNCGW 1 cut(s) 1143
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 1554
PflMI CCANNNNNTGG 1 cut(s) 1703
PfoI TCCNGGA 1 cut(s) 793
PkrI GCNGC 3 cut(s) 51, 1003, 1153
PmeI GTTTAAAC 1 cut(s) 1161
Ppu21I YACGTR 3 cut(s) 630, 1202, 1673
PshBI ATTAAT 1 cut(s) 678
Psp6I CCWGG 5 cut(s) 316, 595, 793, 1032, 1570
PspFI CCCAGC 2 cut(s) 1051, 1173
PspGI CCWGG 5 cut(s) 316, 595, 793, 1032, 1570
PspN4I GGNNCC 3 cut(s) 7, 165, 415
PspPI GGNCC 4 cut(s) 164, 284, 393, 791
PstNI CAGNNNCTG 1 cut(s) 1451
PsuI RGATCY 1 cut(s) 538
RseI CAYNNNNRTG 5 cut(s) 176, 370, 1082, 1412, 1416
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 6 cut(s) 678, 759, 1073, 1091, 1119, 1160
SatI GCNGC 3 cut(s) 50, 1002, 1152
Sau3AI GATC 8 cut(s) 538, 816, 1018, 1302, 1399, 1442, 1498, 1600
Sau96I GGNCC 4 cut(s) 164, 284, 393, 791
ScrFI CCNGG 6 cut(s) 162, 318, 597, 795, 1034, 1572
SduI GDGCHC 1 cut(s) 842
SexAI ACCWGGT 1 cut(s) 316
SfaNI GCATC 4 cut(s) 36, 736, 758, 1378
SfcI CTRYAG 2 cut(s) 1210, 1423
Sfr274I CTCGAG 1 cut(s) 923
SinI GGWCC 3 cut(s) 284, 393, 791
SlaI CTCGAG 1 cut(s) 923
SmiMI CAYNNNNRTG 5 cut(s) 176, 370, 1082, 1412, 1416
SmlI CTYRAG 2 cut(s) 95, 923
SmoI CTYRAG 2 cut(s) 95, 923
SnaBI TACGTA 2 cut(s) 630, 1673
SphI GCATGC 2 cut(s) 139, 380
SspMI CTAG 2 cut(s) 624, 1155
StyD4I CCNGG 6 cut(s) 160, 316, 595, 793, 1032, 1570
StyI CCWWGG 4 cut(s) 272, 409, 1323, 1369
TaaI ACNGT 5 cut(s) 196, 733, 874, 910, 1519
TaiI ACGT 3 cut(s) 632, 1204, 1675
TaqI TCGA 2 cut(s) 924, 1603
TatI WGTACW 4 cut(s) 91, 253, 1086, 1341
TfiI GAWTC 1 cut(s) 1554
Tru1I TTAA 6 cut(s) 678, 759, 1073, 1091, 1119, 1160
Tru9I TTAA 6 cut(s) 678, 759, 1073, 1091, 1119, 1160
TscAI CASTG 3 cut(s) 248, 736, 877
TseFI GTSAC 4 cut(s) 554, 769, 910, 1757
TseI GCWGC 3 cut(s) 49, 1001, 1151
Tsp45I GTSAC 4 cut(s) 554, 769, 910, 1757
TspDTI ATGAA 5 cut(s) 704, 1089, 1362, 1482, 1526
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 3 cut(s) 248, 736, 877
Van91I CCANNNNNTGG 1 cut(s) 1703
VpaK11BI GGWCC 3 cut(s) 284, 393, 791
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 6 cut(s) 325, 699, 918, 1010, 1069, 1456
XceI RCATGY 4 cut(s) 139, 380, 1417, 1693
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 1536
XhoI CTCGAG 1 cut(s) 923
XmiI GTMKAC 1 cut(s) 859
XspI CTAG 2 cut(s) 624, 1155
Zsp2I ATGCAT 2 cut(s) 751, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.