Rroxscaffold_7G00194620

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001075
Physical Location & Seq
Forward (+)
36566652 .. 36578591
11940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00194620.1

Sequence Viewer

Length: 1770 bp
ATGGGTATCTTTCCAGGTTTTCTGGGTTTGTTTCTGCTATGTGGGATGCTGCATTGCATTGTTGAATGCCATGTCCATTACCATGACTTTGTACTCAAAAAGGAGAACTTTACAAGATTATGTTACACAAAGAGCATGCTGGTAGTGAATGGCAGTTTTCCGGGGCCAGTCATTCGTGTGCAAAAGGGGGATACTGTCTTTGTCAATGTTCACAATCAAGGAGATTATGGAGTTACTATTCACTGGCATGGAGTACATCAACCAAGAAATCCATGGTCAGATGGTCCTGAATATATTACACAATGTGCCATCGAAGCTGGCTCAAATTTTACCTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGAATGGACGAGAGCTACTGTCCATGGTGCCATTGTTATTCTGCCTTTAAATAATACGAGTTTTCCGTTTCCTGAACCTGATGGACAAGAAATCATTGTACTCGGTTCTTGGTATAACAGTACTGAAGATGTGAGTGACATAGTTGCTGAAGCCCTAGAAGATGGAGGTGATACTCCCGACTCTGATTGCTACACCATCAATGGACAACCAGGAGATTTTCTCCCATGCTCAAATGATAGTACATACCATTGGGAGGTGGAGTATGGTAAGACCTATCTTCTTCGTCTTGTCAATGCAGTGATGAATGCAGAACTGTTCTTTGCAATTGCTGAACACAGCCTCACCGTTGTTGGTATCGACGCATCCTATGTGAAACCCATCGTGACCGATTATATCATGATTAGCGCTGGACAAACAATGGATATTTTGATCACCGCAAACCAAACCCCTGGCGCTTACTATATGGCTACTAGACAATATAACACTGTGAGGAAAAATATTGATGACTACGATAATTCAAATGTTACTGCAATTCTTCAGTATAAAGAGAAGTACACACCCTTGATGGACCCAGTTTTTCCAAGCATCCTCCCTACTTATTTTGATATAGTATCAGGAAGAAAGTTCACAAAGCGTATTAAGAGCTTGGCAAGCATAGAGCACCCGACAAATGTGCCGAAGAATATATCTACTAGAATGTTTATAACAGCTGCTTCAAATTATGGTTTCATTAATCGTTCTGGCGAGTATGAAACTGGTGTTACTACTAGTCTAAACAATTTAAGTTGGGTTAATCCAGAAACCGATGTCCTACTTTCCTACTACAGGAACATAACAGGGTTTTACACGCCAGACTTTCCTGATTATCCACCATTTATATTTGACTTTGTGTCAGAAGATTTTGCTCCTGATACTGTCCCAACAATAAAGGGGACCAAGGTGAAGGTGTTAGAATACGATGAAGATGTTGAAATAGTGTTTCAGGGTACTGATCTCATGAGAGCATCTGAGGATCATCCAATGCACATGCATGGTTATAGTTTTTATGTGGTTGGATCAGGAACTGGAAATTTTGACAATGAGACAGACCCCAAAGGCTACAATTTATTTGATCCTCCAAAGATGAATACTGTTTCGCTCCCAAAAGTAGGATGGGTAGCTATCAGATTCAAAGCAAGTAACCCTGGAGTTTGGTTCTGGCACTGTCATTTTGATCGACATTTGACTTGGGGTATGAGCTTCGTGATAGTAGTTAAAGATGGAGGTACGCCAGAAACGAGCATACGTAAGCCACCTCCAAACATGCCTCAATGTACTAGCTCATCTACTATTAAGCTGCAACCTTTCCATGATTTCAGCAGAACAGGCGACAAAAGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

589

Amino Acids

66.42

Weight (kDa)

5.1

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 144 3.9e-40 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 8.6e-37 Multicopper oxidase
Cu-oxidase_2 PF07731 416 - 549 1.8e-38 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 822
AclWI GGATC 3 cut(s) 1407, 1450, 1493
AcsI RAATTY 2 cut(s) 325, 1456
AcuI CTGAAG 3 cut(s) 531, 555, 908
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 9 cut(s) 93, 255, 486, 508, 628, 941, 1375, 1654, 1702
AfeI AGCGCT 1 cut(s) 793
AfiI CCNNNNNNNGG 3 cut(s) 640, 1212, 1535
AgsI TTSAA 5 cut(s) 65, 906, 1104, 1358, 1558
AhdI GACNNNNNGTC 1 cut(s) 1276
AhlI ACTAGT 1 cut(s) 1154
AjnI CCWGG 4 cut(s) 13, 595, 835, 1570
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 8 cut(s) 317, 401, 1032, 1097, 1547, 1626, 1707, 1723
AluI AGCT 8 cut(s) 317, 401, 1032, 1097, 1547, 1626, 1707, 1723
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 1 cut(s) 1463
AlwI GGATC 3 cut(s) 1407, 1450, 1493
AlwNI CAGNNNCTG 1 cut(s) 1451
Aor51HI AGCGCT 1 cut(s) 793
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 3 cut(s) 49, 1097, 1723
ApoI RAATTY 2 cut(s) 325, 1456
AseI ATTAAT 1 cut(s) 1119
AspLEI GCGC 2 cut(s) 794, 842
AspS9I GGNCC 4 cut(s) 164, 284, 955, 1320
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 4 cut(s) 566, 721, 811, 1339
AvaII GGWCC 3 cut(s) 284, 955, 1320
BanI GGYRCC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 3 cut(s) 36, 1084, 1710
BccI CCATC 9 cut(s) 275, 317, 461, 542, 590, 773, 946, 1533, 1640
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciT130I CCWGG 4 cut(s) 15, 597, 837, 1572
BciVI GTATCC 1 cut(s) 184
BclI TGATCA 1 cut(s) 816
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 1463
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 6 cut(s) 542, 858, 1080, 1155, 1704, 1768
BfmI CTRYAG 1 cut(s) 1210
BfoI RGCGCY 2 cut(s) 795, 843
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 3 cut(s) 50, 1098, 1724
BlsI GCNGC 3 cut(s) 51, 1099, 1725
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 5 cut(s) 15, 162, 597, 837, 1572
Bme18I GGWCC 3 cut(s) 284, 955, 1320
BmeRI GACNNNNNGTC 1 cut(s) 1276
BmgT120I GGNCC 4 cut(s) 164, 284, 955, 1320
BmiI GGNNCC 4 cut(s) 165, 415, 957, 1321
BmrFI CCNGG 5 cut(s) 15, 162, 597, 837, 1572
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 4 cut(s) 36, 758, 981, 1400
BmuI ACTGGG 1 cut(s) 953
BplI GAGNNNNNCTC 2 cut(s) 591, 623
BpmI CTGGAG 1 cut(s) 1593
BpuMI CCSGG 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 1673
BsaJI CCNNGG 6 cut(s) 161, 272, 409, 835, 1323, 1570
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 3 cut(s) 640, 1212, 1535
Bse1I ACTGG 5 cut(s) 167, 248, 959, 1147, 1456
Bse3DI GCAATG 1 cut(s) 52
BseBI CCWGG 4 cut(s) 15, 597, 837, 1572
BseDI CCNNGG 6 cut(s) 161, 272, 409, 835, 1323, 1570
BseGI GGATG 5 cut(s) 51, 749, 972, 1402, 1544
BseLI CCNNNNNNNGG 3 cut(s) 640, 1212, 1535
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 1386
BseNI ACTGG 5 cut(s) 167, 248, 959, 1147, 1456
BseXI GCAGC 3 cut(s) 36, 1084, 1710
BshFI GGCC 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 1050
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 2 cut(s) 1289, 1333
BslI CCNNNNNNNGG 3 cut(s) 640, 1212, 1535
BsmAI GTCTC 1 cut(s) 1463
BsmFI GGGAC 2 cut(s) 1289, 1333
BsmI GAATGC 2 cut(s) 71, 697
BsnI GGCC 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 1050
Bsp143I GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
Bsp19I CCATGG 2 cut(s) 272, 409
BspACI CCGC 1 cut(s) 822
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 1 cut(s) 1387
BspHI TCATGA 2 cut(s) 783, 1383
BspLI GGNNCC 4 cut(s) 165, 415, 957, 1321
BspPI GGATC 3 cut(s) 1407, 1450, 1493
BspT107I GGYRCC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 52
BsrI ACTGG 5 cut(s) 167, 248, 959, 1147, 1456
BssECI CCNNGG 6 cut(s) 161, 272, 409, 835, 1323, 1570
BssMI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
BssT1I CCWWGG 3 cut(s) 272, 409, 1323
Bst2UI CCWGG 4 cut(s) 15, 597, 837, 1572
Bst4CI ACNGT 9 cut(s) 196, 406, 506, 702, 733, 874, 1303, 1519, 1592
BstBAI YACGTR 1 cut(s) 1673
BstC8I GCNNGC 4 cut(s) 137, 319, 378, 1039
BstDEI CTNAG 1 cut(s) 1395
BstDSI CCRYGG 2 cut(s) 272, 409
BstENI CCTNNNNNAGG 1 cut(s) 1210
BstF5I GGATG 5 cut(s) 51, 749, 972, 1402, 1544
BstH2I RGCGCY 2 cut(s) 795, 843
BstHHI GCGC 2 cut(s) 794, 842
BstKTI GATC 6 cut(s) 819, 1381, 1402, 1445, 1501, 1603
BstMAI GTCTC 1 cut(s) 1463
BstMBI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
BstMWI GCNNNNNNNGC 3 cut(s) 314, 1038, 1752
BstNI CCWGG 4 cut(s) 15, 597, 837, 1572
BstNSI RCATGY 4 cut(s) 139, 380, 1417, 1693
BstSCI CCNGG 5 cut(s) 13, 160, 595, 835, 1570
BstSFI CTRYAG 1 cut(s) 1210
BstSNI TACGTA 1 cut(s) 1673
BstV1I GCAGC 3 cut(s) 36, 1084, 1710
BstXI CCANNNNNNTGG 1 cut(s) 836
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 166
BtgI CCRYGG 2 cut(s) 272, 409
BtsCI GGATG 5 cut(s) 51, 749, 972, 1402, 1544
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 4 cut(s) 241, 690, 870, 1588
Cac8I GCNNGC 4 cut(s) 137, 319, 378, 1039
CaiI CAGNNNCTG 1 cut(s) 1451
CciI TCATGA 2 cut(s) 783, 1383
CfoI GCGC 2 cut(s) 794, 842
Cfr13I GGNCC 4 cut(s) 164, 284, 955, 1320
CseI GACGC 1 cut(s) 755
Csp6I GTAC 9 cut(s) 92, 254, 485, 507, 627, 940, 1374, 1653, 1701
CviQI GTAC 9 cut(s) 92, 254, 485, 507, 627, 940, 1374, 1653, 1701
DdeI CTNAG 1 cut(s) 1395
DpnI GATC 6 cut(s) 818, 1380, 1401, 1444, 1500, 1602
DpnII GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
DraI TTTAAA 1 cut(s) 435
DraIII CACNNNGTG 1 cut(s) 305
DriI GACNNNNNGTC 1 cut(s) 1276
Eam1105I GACNNNNNGTC 1 cut(s) 1276
Eco105I TACGTA 1 cut(s) 1673
Eco130I CCWWGG 3 cut(s) 272, 409, 1323
Eco47I GGWCC 3 cut(s) 284, 955, 1320
Eco47III AGCGCT 1 cut(s) 793
Eco57I CTGAAG 3 cut(s) 531, 555, 908
EcoNI CCTNNNNNAGG 1 cut(s) 1210
EcoRII CCWGG 4 cut(s) 13, 595, 835, 1570
EcoT14I CCWWGG 3 cut(s) 272, 409, 1323
EcoT22I ATGCAT 1 cut(s) 1419
ErhI CCWWGG 3 cut(s) 272, 409, 1323
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 351, 383
FaqI GGGAC 2 cut(s) 1289, 1333
FbaI TGATCA 1 cut(s) 816
Fnu4HI GCNGC 3 cut(s) 50, 1098, 1724
FokI GGATG 5 cut(s) 58, 736, 959, 1389, 1551
Fsp4HI GCNGC 3 cut(s) 50, 1098, 1724
FspBI CTAG 6 cut(s) 542, 858, 1080, 1155, 1704, 1768
GlaI GCGC 2 cut(s) 793, 841
GluI GCNGC 3 cut(s) 50, 1098, 1724
GsuI CTGGAG 1 cut(s) 1593
HaeII RGCGCY 2 cut(s) 795, 843
HaeIII GGCC 1 cut(s) 166
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 755
HhaI GCGC 2 cut(s) 794, 842
Hin6I GCGC 2 cut(s) 792, 840
HinP1I GCGC 2 cut(s) 792, 840
HinfI GANTC 2 cut(s) 566, 1554
HpaII CCGG 1 cut(s) 161
HphI GGTGA 4 cut(s) 566, 721, 811, 1339
Hpy166II GTNNAC 3 cut(s) 211, 942, 1014
Hpy188I TCNGA 6 cut(s) 280, 352, 571, 1282, 1396, 1553
Hpy8I GTNNAC 3 cut(s) 211, 942, 1014
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 353, 1324
HpyCH4III ACNGT 9 cut(s) 196, 406, 506, 702, 733, 874, 1303, 1519, 1592
HpyCH4IV ACGT 1 cut(s) 1672
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 1038, 1752
HpyF3I CTNAG 1 cut(s) 1395
HpySE526I ACGT 1 cut(s) 1672
HspAI GCGC 2 cut(s) 792, 840
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
LmnI GCTCC 2 cut(s) 1297, 1530
Lsp1109I GCAGC 3 cut(s) 36, 1084, 1710
LweI GCATC 4 cut(s) 36, 758, 981, 1400
MaeI CTAG 6 cut(s) 542, 858, 1080, 1155, 1704, 1768
MaeII ACGT 1 cut(s) 1672
MaeIII GTNAC 8 cut(s) 122, 232, 521, 769, 910, 1147, 1565, 1763
MalI GATC 6 cut(s) 818, 1380, 1401, 1444, 1500, 1602
MboI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
MboII GAAGA 9 cut(s) 524, 557, 656, 659, 914, 1017, 1078, 1295, 1361
MfeI CAATTG 1 cut(s) 711
MhlI GDGCHC 1 cut(s) 1050
MluCI AATT 8 cut(s) 325, 711, 901, 918, 1105, 1165, 1456, 1489
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 1 cut(s) 1420
Mph1103I ATGCAT 1 cut(s) 1419
MseI TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
MslI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 5 cut(s) 15, 162, 597, 837, 1572
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 2 cut(s) 71, 697
MvaI CCWGG 4 cut(s) 15, 597, 837, 1572
MwoI GCNNNNNNNGC 3 cut(s) 314, 1038, 1752
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 2 cut(s) 272, 409
NdeII GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
NlaIV GGNNCC 4 cut(s) 165, 415, 957, 1321
NmuCI GTSAC 2 cut(s) 521, 769
NsiI ATGCAT 1 cut(s) 1419
NspI RCATGY 4 cut(s) 139, 380, 1417, 1693
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 2 cut(s) 139, 380
PagI TCATGA 2 cut(s) 783, 1383
PcsI WCGNNNNNNNCGW 2 cut(s) 449, 1661
PctI GAATGC 2 cut(s) 71, 697
PfeI GAWTC 1 cut(s) 1554
PkrI GCNGC 3 cut(s) 51, 1099, 1725
PleI GAGTC 1 cut(s) 560
PpsI GAGTC 1 cut(s) 560
Ppu21I YACGTR 1 cut(s) 1673
PshBI ATTAAT 1 cut(s) 1119
PsiI TTATAA 1 cut(s) 1091
Psp6I CCWGG 4 cut(s) 13, 595, 835, 1570
PspGI CCWGG 4 cut(s) 13, 595, 835, 1570
PspN4I GGNNCC 4 cut(s) 165, 415, 957, 1321
PspPI GGNCC 4 cut(s) 164, 284, 955, 1320
PstNI CAGNNNCTG 1 cut(s) 1451
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 9 cut(s) 93, 255, 486, 508, 628, 941, 1375, 1654, 1702
RsaNI GTAC 9 cut(s) 92, 254, 485, 507, 627, 940, 1374, 1653, 1701
RseI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
SaqAI TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
SatI GCNGC 3 cut(s) 50, 1098, 1724
Sau3AI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
Sau96I GGNCC 4 cut(s) 164, 284, 955, 1320
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 5 cut(s) 15, 162, 597, 837, 1572
SduI GDGCHC 1 cut(s) 1050
SfaNI GCATC 4 cut(s) 36, 758, 981, 1400
SfcI CTRYAG 1 cut(s) 1210
SinI GGWCC 3 cut(s) 284, 955, 1320
SmiMI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
SnaBI TACGTA 1 cut(s) 1673
SpeI ACTAGT 1 cut(s) 1154
SphI GCATGC 2 cut(s) 139, 380
Sse9I AATT 8 cut(s) 325, 711, 901, 918, 1105, 1165, 1456, 1489
SsiI CCGC 1 cut(s) 822
SspI AATATT 1 cut(s) 886
SspMI CTAG 6 cut(s) 542, 858, 1080, 1155, 1704, 1768
StyD4I CCNGG 5 cut(s) 13, 160, 595, 835, 1570
StyI CCWWGG 3 cut(s) 272, 409, 1323
TaaI ACNGT 9 cut(s) 196, 406, 506, 702, 733, 874, 1303, 1519, 1592
TaiI ACGT 1 cut(s) 1675
TaqI TCGA 3 cut(s) 312, 744, 1603
TaqII GACCGA 1 cut(s) 788
TasI AATT 8 cut(s) 325, 711, 901, 918, 1105, 1165, 1456, 1489
TatI WGTACW 7 cut(s) 91, 253, 484, 506, 626, 939, 1700
TfiI GAWTC 1 cut(s) 1554
Tru1I TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
Tru9I TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
TscAI CASTG 4 cut(s) 248, 690, 877, 1595
TseFI GTSAC 2 cut(s) 521, 769
TseI GCWGC 3 cut(s) 49, 1097, 1723
Tsp45I GTSAC 2 cut(s) 521, 769
TspDTI ATGAA 5 cut(s) 704, 1105, 1152, 1362, 1526
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 4 cut(s) 248, 690, 877, 1595
VpaK11BI GGWCC 3 cut(s) 284, 955, 1320
VspI ATTAAT 1 cut(s) 1119
XagI CCTNNNNNAGG 1 cut(s) 1210
XapI RAATTY 2 cut(s) 325, 1456
XceI RCATGY 4 cut(s) 139, 380, 1417, 1693
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 1536
XspI CTAG 6 cut(s) 542, 858, 1080, 1155, 1704, 1768
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.