Rh6DG187300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001075
Physical Location & Seq
Reverse (-)
33330751 .. 33362305
31555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG187300.1

Sequence Viewer

Length: 1689 bp
ATGGGTGTGCTCCAATGGTTGCTGGGTTCTCTGCTTCTATGCAGTGCGCTTCTTTGCATAGTTGAAGGAAATGTTCACTACTATGACTTTGTCCTCAAGGAGGCCAACTTTACAAGGTTGTGTCAGACTAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGTCATTCGTGTACAAAAAGGTGATACTGTTTATGTCAATGTCCAAAATCAAGGAGCCTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCAAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACGCAGTGTCCAATTGAACCTGGATCAAATTTCACTTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGATTGGACCAGAGCTAGTGTCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACAGGTTTTCCATTTCCCGAGCCAGATGGAGAAGAAATAGTTGTACTTGGTTCTTGGTATTATATTGATAATCTGAATGAGGTGGTTGCTAAGGACGTCGAAGAAGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTCAGCCTATGCTCAAATGACAGTACGTATCGCTGGCAAGTGGAGTATGGCAAGACGTACCTTCTTCGCCTGGTTAGTGCAGTTATGAATGCAGAACTCTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTAAAACCGATTGTGACCAATAATATCATGATTTCCCCCGGACAAACCATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGTGTTAGGCCAGATGATCAAGACTATGACAAGTCAAATGTTACAGCAATTCTGGAGTATAAAGGCAATTACACAACCCCTGCAGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCGATAGAGTTTACAGACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGATGATGTGACAGTTGCTCTTCTTGCAAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGAAAATTTGCCTGATAATACTGAGGTTACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATAGTGTTTCAGGGTACTAATACAATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGATATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGACAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTATTTCAGTCCCAATGAAAGGATGGGTTGCTATCAGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGAGGCACAGCGGAGAGGTGCATGCGAGAACCTCCTCCATACATGCCTCCCTGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

562

Amino Acids

62.83

Weight (kDa)

4.62

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 146 4e-40 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 5.3e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 425 - 519 1.1e-22 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 2 cut(s) 1094, 1641
AclWI GGATC 5 cut(s) 328, 1453, 1472, 1496, 1591
AcsI RAATTY 3 cut(s) 325, 1288, 1459
AcyI GRCGYC 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 8 cut(s) 180, 486, 625, 659, 973, 1088, 1346, 1378
AfiI CCNNNNNNNGG 3 cut(s) 100, 1215, 1538
AgsI TTSAA 2 cut(s) 65, 314
AjnI CCWGG 4 cut(s) 160, 316, 592, 669
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 7 cut(s) 401, 821, 1032, 1176, 1431, 1488, 1609
AluI AGCT 7 cut(s) 401, 821, 1032, 1176, 1431, 1488, 1609
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 434, 834
AlwI GGATC 5 cut(s) 328, 1453, 1472, 1496, 1591
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 4 cut(s) 102, 164, 229, 875
ApeKI GCWGC 2 cut(s) 851, 950
ApoI RAATTY 3 cut(s) 325, 1288, 1459
AseI ATTAAT 1 cut(s) 1389
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 164, 284, 393
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 2 cut(s) 200, 1078
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 2 cut(s) 284, 393
BanI GGYRCC 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 964, 996
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 838, 962
BccI CCATC 4 cut(s) 275, 461, 737, 1536
BciT130I CCWGG 4 cut(s) 162, 318, 594, 671
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 2 cut(s) 434, 834
BfaI CTAG 2 cut(s) 402, 855
BfmI CTRYAG 3 cut(s) 948, 1191, 1213
BfuAI ACCTGC 1 cut(s) 1009
BisI GCNGC 2 cut(s) 852, 951
BlpI GCTNAGC 1 cut(s) 1033
BlsI GCNGC 2 cut(s) 853, 952
Bme1390I CCNGG 5 cut(s) 162, 318, 594, 671, 792
Bme18I GGWCC 2 cut(s) 284, 393
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 3 cut(s) 164, 284, 393
BmiI GGNNCC 3 cut(s) 223, 269, 415
BmrFI CCNGG 5 cut(s) 162, 318, 594, 671, 792
BmsI GCATC 2 cut(s) 265, 1403
BpmI CTGGAG 1 cut(s) 941
Bpu10I CCTNAGC 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 1033
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 792
BsaAI YACGTR 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 801, 1326
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 1215, 1538
Bse118I RCCGGY 1 cut(s) 1063
Bse1I ACTGG 3 cut(s) 167, 248, 826
BseBI CCWGG 4 cut(s) 162, 318, 594, 671
BseDI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 801, 1326
BseGI GGATG 3 cut(s) 748, 1405, 1547
BseLI CCNNNNNNNGG 3 cut(s) 100, 1215, 1538
BseMII CTCAG 3 cut(s) 279, 1254, 1296
BseNI ACTGG 3 cut(s) 167, 248, 826
BseRI GAGGAG 2 cut(s) 549, 1654
BseXI GCAGC 2 cut(s) 838, 962
BseYI CCCAGC 2 cut(s) 22, 1176
BsgI GTGCAG 2 cut(s) 699, 1667
BshFI GGCC 4 cut(s) 104, 166, 231, 877
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 792, 1064
BslFI GGGAC 1 cut(s) 1514
BslI CCNNNNNNNGG 3 cut(s) 100, 1215, 1538
BsmAI GTCTC 2 cut(s) 434, 834
BsmFI GGGAC 1 cut(s) 1514
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 4 cut(s) 104, 166, 231, 877
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1407I TGTACA 2 cut(s) 178, 1086
Bsp143I GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
Bsp1720I GCTNAGC 1 cut(s) 1033
Bsp19I CCATGG 2 cut(s) 409, 801
BspACI CCGC 2 cut(s) 1094, 1641
BspANI GGCC 4 cut(s) 104, 166, 231, 877
BspCNI CTCAG 3 cut(s) 280, 1253, 1297
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 3 cut(s) 223, 269, 415
BspMAI CTGCAG 1 cut(s) 952
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 5 cut(s) 328, 1453, 1472, 1496, 1591
BspQI GCTCTTC 1 cut(s) 1155
BspT107I GGYRCC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 1063
BsrGI TGTACA 2 cut(s) 178, 1086
BsrI ACTGG 3 cut(s) 167, 248, 826
BssAI RCCGGY 1 cut(s) 1063
BssECI CCNNGG 7 cut(s) 161, 263, 272, 409, 790, 801, 1326
BssMI GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
BssNI GRCGYC 1 cut(s) 537
BssT1I CCWWGG 5 cut(s) 263, 272, 409, 801, 1326
Bst2UI CCWGG 4 cut(s) 162, 318, 594, 671
Bst4CI ACNGT 7 cut(s) 155, 196, 623, 730, 1144, 1315, 1575
Bst6I CTCTTC 2 cut(s) 1155, 1347
BstACI GRCGYC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 2 cut(s) 178, 1086
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 5 cut(s) 137, 378, 635, 1039, 1653
BstDEI CTNAG 6 cut(s) 129, 288, 531, 1033, 1240, 1305
BstDSI CCRYGG 2 cut(s) 409, 801
BstENI CCTNNNNNAGG 2 cut(s) 98, 1213
BstF5I GGATG 3 cut(s) 748, 1405, 1547
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 6 cut(s) 323, 886, 1448, 1480, 1504, 1586
BstMAI GTCTC 2 cut(s) 434, 834
BstMBI GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
BstMWI GCNNNNNNNGC 4 cut(s) 253, 570, 1038, 1154
BstNI CCWGG 4 cut(s) 162, 318, 594, 671
BstNSI RCATGY 4 cut(s) 139, 380, 1655, 1676
BstSCI CCNGG 5 cut(s) 160, 316, 592, 669, 790
BstSFI CTRYAG 3 cut(s) 948, 1191, 1213
BstSNI TACGTA 1 cut(s) 627
BstV1I GCAGC 2 cut(s) 838, 962
BstX2I RGATCY 1 cut(s) 1477
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 1477
BsuRI GGCC 4 cut(s) 104, 166, 231, 877
BtgI CCRYGG 2 cut(s) 409, 801
BtsCI GGATG 3 cut(s) 748, 1405, 1547
BtsI GCAGTG 2 cut(s) 49, 308
BtsIMutI CAGTG 6 cut(s) 49, 241, 308, 726, 1571, 1684
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 5 cut(s) 137, 378, 635, 1039, 1653
CciI TCATGA 1 cut(s) 780
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 1063
Cfr13I GGNCC 3 cut(s) 164, 284, 393
Csp6I GTAC 8 cut(s) 179, 485, 624, 658, 972, 1087, 1345, 1377
CviQI GTAC 8 cut(s) 179, 485, 624, 658, 972, 1087, 1345, 1377
DdeI CTNAG 6 cut(s) 129, 288, 531, 1033, 1240, 1305
DpnI GATC 6 cut(s) 322, 885, 1447, 1479, 1503, 1585
DpnII GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
DraI TTTAAA 1 cut(s) 1164
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 1155, 1347
EarI CTCTTC 2 cut(s) 1155, 1347
Eco105I TACGTA 1 cut(s) 627
Eco130I CCWWGG 5 cut(s) 263, 272, 409, 801, 1326
Eco47I GGWCC 2 cut(s) 284, 393
Eco88I CYCGRG 1 cut(s) 458
EcoNI CCTNNNNNAGG 2 cut(s) 98, 1213
EcoRII CCWGG 4 cut(s) 160, 316, 592, 669
EcoT14I CCWWGG 5 cut(s) 263, 272, 409, 801, 1326
EcoT22I ATGCAT 1 cut(s) 1396
ErhI CCWWGG 5 cut(s) 263, 272, 409, 801, 1326
FalI AAGNNNNNCTT 4 cut(s) 256, 288, 351, 383
FaqI GGGAC 1 cut(s) 1514
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 2 cut(s) 852, 951
FokI GGATG 3 cut(s) 755, 1392, 1554
Fsp4HI GCNGC 2 cut(s) 852, 951
FspBI CTAG 2 cut(s) 402, 855
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 852, 951
GsaI CCCAGC 2 cut(s) 26, 1180
GsuI CTGGAG 1 cut(s) 941
HaeIII GGCC 4 cut(s) 104, 166, 231, 877
HapII CCGG 2 cut(s) 792, 1064
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 537
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 1 cut(s) 1402
HpaII CCGG 2 cut(s) 792, 1064
HphI GGTGA 2 cut(s) 200, 1078
Hpy166II GTNNAC 3 cut(s) 76, 179, 1014
Hpy188III TCNNGA 6 cut(s) 287, 458, 781, 887, 920, 1027
Hpy8I GTNNAC 3 cut(s) 76, 179, 1014
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 5 cut(s) 59, 353, 539, 671, 990
HpyCH4III ACNGT 7 cut(s) 155, 196, 623, 730, 1144, 1315, 1575
HpyCH4IV ACGT 3 cut(s) 537, 626, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 570, 1038, 1154
HpyF3I CTNAG 6 cut(s) 129, 288, 531, 1033, 1240, 1305
HpySE526I ACGT 3 cut(s) 537, 626, 656
Hsp92I GRCGYC 1 cut(s) 537
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 3 cut(s) 15, 221, 746
Lsp1109I GCAGC 2 cut(s) 838, 962
LweI GCATC 2 cut(s) 265, 1403
MaeI CTAG 2 cut(s) 402, 855
MaeII ACGT 3 cut(s) 537, 626, 656
MaeIII GTNAC 6 cut(s) 295, 766, 814, 907, 1138, 1309
MalI GATC 6 cut(s) 322, 885, 1447, 1479, 1503, 1585
MboI GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
MboII GAAGA 7 cut(s) 485, 554, 656, 1142, 1364, 1522, 1637
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 1477
MhlI GDGCHC 1 cut(s) 12
MmeI TCCRAC 2 cut(s) 1211, 1423
Mph1103I ATGCAT 1 cut(s) 1396
MseI TTAA 3 cut(s) 1163, 1182, 1389
MslI CAYNNNNRTG 4 cut(s) 81, 246, 370, 1419
MspA1I CMGCKG 1 cut(s) 1641
MspI CCGG 2 cut(s) 792, 1064
MspR9I CCNGG 5 cut(s) 162, 318, 594, 671, 792
MssI GTTTAAAC 1 cut(s) 1164
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 4 cut(s) 162, 318, 594, 671
MwoI GCNNNNNNNGC 4 cut(s) 253, 570, 1038, 1154
NciI CCSGG 1 cut(s) 792
NcoI CCATGG 2 cut(s) 409, 801
NdeI CATATG 1 cut(s) 751
NdeII GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
NlaIV GGNNCC 3 cut(s) 223, 269, 415
NmuCI GTSAC 4 cut(s) 295, 766, 814, 1138
NsiI ATGCAT 1 cut(s) 1396
NspI RCATGY 4 cut(s) 139, 380, 1655, 1676
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 3 cut(s) 139, 380, 1655
PagI TCATGA 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 1155
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 1 cut(s) 1402
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 853, 952
PmeI GTTTAAAC 1 cut(s) 1164
Ppu21I YACGTR 1 cut(s) 627
PshBI ATTAAT 1 cut(s) 1389
Psp6I CCWGG 4 cut(s) 160, 316, 592, 669
PspFI CCCAGC 2 cut(s) 22, 1176
PspGI CCWGG 4 cut(s) 160, 316, 592, 669
PspN4I GGNNCC 3 cut(s) 223, 269, 415
PspPI GGNCC 3 cut(s) 164, 284, 393
PstI CTGCAG 1 cut(s) 952
PsuI RGATCY 1 cut(s) 1477
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 8 cut(s) 180, 486, 625, 659, 973, 1088, 1346, 1378
RsaNI GTAC 8 cut(s) 179, 485, 624, 658, 972, 1087, 1345, 1377
RseI CAYNNNNRTG 4 cut(s) 81, 246, 370, 1419
SapI GCTCTTC 1 cut(s) 1155
SaqAI TTAA 3 cut(s) 1163, 1182, 1389
SatI GCNGC 2 cut(s) 852, 951
Sau3AI GATC 6 cut(s) 320, 883, 1445, 1477, 1501, 1583
Sau96I GGNCC 3 cut(s) 164, 284, 393
ScrFI CCNGG 5 cut(s) 162, 318, 594, 671, 792
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 2 cut(s) 265, 1403
SfcI CTRYAG 3 cut(s) 948, 1191, 1213
SinI GGWCC 2 cut(s) 284, 393
SmiMI CAYNNNNRTG 4 cut(s) 81, 246, 370, 1419
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SnaBI TACGTA 1 cut(s) 627
SphI GCATGC 3 cut(s) 139, 380, 1655
SsiI CCGC 2 cut(s) 1094, 1641
SspMI CTAG 2 cut(s) 402, 855
StyD4I CCNGG 5 cut(s) 160, 316, 592, 669, 790
StyI CCWWGG 5 cut(s) 263, 272, 409, 801, 1326
TaaI ACNGT 7 cut(s) 155, 196, 623, 730, 1144, 1315, 1575
TaiI ACGT 3 cut(s) 540, 629, 659
TaqI TCGA 2 cut(s) 540, 1586
TatI WGTACW 4 cut(s) 178, 484, 1086, 1344
TfiI GAWTC 1 cut(s) 1402
Tru1I TTAA 3 cut(s) 1163, 1182, 1389
Tru9I TTAA 3 cut(s) 1163, 1182, 1389
TscAI CASTG 5 cut(s) 49, 248, 308, 733, 1578
TseFI GTSAC 4 cut(s) 295, 766, 814, 1138
TseI GCWGC 2 cut(s) 851, 950
Tsp45I GTSAC 4 cut(s) 295, 766, 814, 1138
TspDTI ATGAA 6 cut(s) 596, 701, 1118, 1299, 1365, 1550
TspRI CASTG 5 cut(s) 49, 248, 308, 733, 1578
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 284, 393
VspI ATTAAT 1 cut(s) 1389
XagI CCTNNNNNAGG 2 cut(s) 98, 1213
XapI RAATTY 3 cut(s) 325, 1288, 1459
XceI RCATGY 4 cut(s) 139, 380, 1655, 1676
XcmI CCANNNNNNNNNTGG 3 cut(s) 270, 808, 1539
XspI CTAG 2 cut(s) 402, 855
ZraI GACGTC 1 cut(s) 538
Zsp2I ATGCAT 1 cut(s) 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.