RchiOBHm_Chr2g0147191

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001075
Physical Location & Seq
Reverse (-)
64849907 .. 64852667
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51684

Sequence Viewer

Length: 1632 bp
ATGCTAGTAGTCAACGATAGTTTTCCTGGACCGACCATTCGCGTGCAGAAAGGAGACACCGTCTATGTCAATGTCCAAAACCAAGGAGACTATGGCCTTACTATTCACTGGCATGGAGTACATCAACCAAGAAATCCTTGGTCAGATGGTCCAGAATATGTTACACAATGTCCTATTGAGCCTAAAACAAATTTCACTTATGAGGTTATATTTTCTGATGAGGAAGGAACACTATGGTGGCATGCCCATAGTGATTGGACTAGAGCTAGTGTCCATGGTGCCATTGTTGTTATGCCCTTAGAAGAGACAGGTTTTCCATTTGCTGAGCCCGATGGAGAAGAATTAATCATATTTGGATCTTGGTATTTAATTGAACGTCTGAACGAGGTGGTTACTGAGGATGTCGAAGAAGGTCTTGACACTCCTCGGTCTGATTGCTACACCATTAATGGACAACCTGGAGATTTTGGCTGGTGCTCAAATGAAAGTACACATCGCTGGCAAGTAGAGTATAACAAGACGTATCTTCTTCGTATTGTCAATGCTGTGATGAATGCAGAACTCTACTTTGCCATTGCTGAACATACACTCACTGTTGTTGGTATGGATGGATCATATGTTAAACCCATCGTGACCGATTATGTTATGATTTCTGCCGGACAAACCATGGATATTTTGGTCACCGCCAACCAGCCTCTTGGCCGATACTACATGGCTGGAAGACAATTTGACACCGCTAAGTCAGCCGTTGAAGGCTATGACAAATCAAATGTGACAGCAATTCTGGAGTATAAAGGCAATTACTCTCACCCGGTATACCCAATCTTTCCATCCACCCTTCCTGCGTTTGAGGATATAGAATCTGCGGTAAAGTTCACAAGTCGTCTTAAGAGCTTAGCAAGCATAGACCACCCTACAAATGTGCCACAGAATATAACTACCAAAATGTATATAACGGTTGCGATGAATAATGGCACCCTTATTAATGGTGGTGAAGAGATCAAAAGTGGCCTTCTATCAAGTTTAAACAATCTAAGTTGGGTTAATCCATCAACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGATTTTATACTTCAGATTTTCCTGATTTTCCACCAGCATTATTTGACTTCGTATCTGATGATCTGGCTCCTGATAGAACTATTGCAACTGTCAAAGCAACTAGAGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATTGTATTTCAGGGTACTAATTTGCTTGATGCATCTGAGGATCATCCAATGCACTTGCATGGTTATAGCTTTTATGTAGTTGGATCAGGTTTTGGAGATTTTGACAACGAGACAGACCCTGAAGGGTATAATTTGATTGATCCACCAAAGCTAACTACTGTTTCAGTCCCAAAGAAAGGGTGGGTTGCTATCAGATTCAAAGCCAGTAATCCAGGGGTCTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGTATGAACACTGTGATGATAGTGAAAAATGGTGGCACGCCAGAGACGAGCATACGCAGACCACCTCCATACTTGCCTCCATGTAATGGTTCACCTGCCAACGTGTTCGAGCAGTTTCACCACAAATTCAGCAAGACAAGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

543

Amino Acids

61.04

Weight (kDa)

4.96

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 2 - 99 1.2e-37 Multicopper oxidase
Cu-oxidase PF00394 115 - 267 4.3e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 371 - 505 1.5e-38 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1588
Acc36I ACCTGC 1 cut(s) 1588
AccB1I GGYRCC 2 cut(s) 278, 974
AccB7I CCANNNNNTGG 1 cut(s) 1571
AccI GTMKAC 1 cut(s) 816
AccII CGCG 1 cut(s) 42
AciI CCGC 3 cut(s) 684, 735, 866
AclWI GGATC 6 cut(s) 364, 619, 1275, 1318, 1361, 1476
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 2 cut(s) 190, 1610
AcuI CTGAAG 2 cut(s) 1086, 1368
AfaI GTAC 4 cut(s) 120, 490, 1211, 1243
AfiI CCNNNNNNNGG 3 cut(s) 1077, 1403, 1571
AflII CTTAAG 1 cut(s) 887
AflIII ACRYGT 1 cut(s) 1587
AgsI TTSAA 3 cut(s) 374, 752, 1426
AjnI CCWGG 3 cut(s) 25, 457, 1438
AleI CACNNNNGTG 1 cut(s) 235
AluBI AGCT 4 cut(s) 266, 894, 1296, 1378
AluI AGCT 4 cut(s) 266, 894, 1296, 1378
Alw21I GWGCWC 1 cut(s) 479
Alw26I GTCTC 5 cut(s) 48, 81, 299, 1331, 1523
AlwI GGATC 6 cut(s) 364, 619, 1275, 1318, 1361, 1476
AlwNI CAGNNNCTG 1 cut(s) 1346
AoxI GGCC 3 cut(s) 94, 700, 1009
ApoI RAATTY 2 cut(s) 190, 1610
ArsI GACNNNNNNTTYG 2 cut(s) 21, 53
AseI ATTAAT 3 cut(s) 344, 447, 984
AspS9I GGNCC 2 cut(s) 29, 149
AsuC2I CCSGG 1 cut(s) 812
AsuHPI GGTGA 5 cut(s) 673, 800, 1004, 1569, 1595
AvaII GGWCC 2 cut(s) 29, 149
BanI GGYRCC 2 cut(s) 278, 974
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 727
Bbv12I GWGCWC 1 cut(s) 479
BccI CCATC 6 cut(s) 140, 326, 602, 635, 838, 1057
BceAI ACGGC 1 cut(s) 731
BciT130I CCWGG 3 cut(s) 27, 459, 1440
BcnI CCSGG 1 cut(s) 812
BcoDI GTCTC 5 cut(s) 48, 81, 299, 1331, 1523
BfaI CTAG 4 cut(s) 5, 261, 267, 1191
BfmI CTRYAG 1 cut(s) 1075
BfrI CTTAAG 1 cut(s) 887
BfuAI ACCTGC 1 cut(s) 1588
BlpI GCTNAGC 2 cut(s) 324, 895
Bme1390I CCNGG 4 cut(s) 27, 459, 812, 1440
Bme18I GGWCC 2 cut(s) 29, 149
BmgT120I GGNCC 2 cut(s) 29, 149
BmiI GGNNCC 3 cut(s) 280, 976, 1158
BmrFI CCNGG 4 cut(s) 27, 459, 812, 1440
BmsI GCATC 2 cut(s) 1246, 1268
BpiI GAAGAC 1 cut(s) 727
BpmI CTGGAG 2 cut(s) 480, 806
Bpu1102I GCTNAGC 2 cut(s) 324, 895
BpuMI CCSGG 1 cut(s) 812
BsaJI CCNNGG 6 cut(s) 82, 137, 274, 425, 666, 1439
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 3 cut(s) 1077, 1403, 1571
Bse1I ACTGG 2 cut(s) 113, 1431
Bse3DI GCAATG 1 cut(s) 573
BseBI CCWGG 3 cut(s) 27, 459, 1440
BseDI CCNNGG 6 cut(s) 82, 137, 274, 425, 666, 1439
BseGI GGATG 4 cut(s) 406, 613, 830, 1270
BseLI CCNNNNNNNGG 3 cut(s) 1077, 1403, 1571
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 3 cut(s) 315, 387, 1254
BseNI ACTGG 2 cut(s) 113, 1431
BseRI GAGGAG 1 cut(s) 414
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 96, 702, 1011
BshNI GGYRCC 2 cut(s) 278, 974
BsiHKAI GWGCWC 1 cut(s) 479
BsiSI CCGG 2 cut(s) 657, 812
BslFI GGGAC 1 cut(s) 1379
BslI CCNNNNNNNGG 3 cut(s) 1077, 1403, 1571
BsmAI GTCTC 5 cut(s) 48, 81, 299, 1331, 1523
BsmBI CGTCTC 1 cut(s) 1523
BsmFI GGGAC 1 cut(s) 1379
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 3 cut(s) 96, 702, 1011
Bsp1286I GDGCHC 2 cut(s) 330, 479
Bsp143I GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
Bsp1720I GCTNAGC 2 cut(s) 324, 895
Bsp19I CCATGG 2 cut(s) 274, 666
BspACI CCGC 3 cut(s) 684, 735, 866
BspANI GGCC 3 cut(s) 96, 702, 1011
BspCNI CTCAG 3 cut(s) 316, 388, 1255
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 3 cut(s) 280, 976, 1158
BspMI ACCTGC 1 cut(s) 1588
BspPI GGATC 6 cut(s) 364, 619, 1275, 1318, 1361, 1476
BspT107I GGYRCC 2 cut(s) 278, 974
BspTI CTTAAG 1 cut(s) 887
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 2 cut(s) 113, 1431
BssECI CCNNGG 6 cut(s) 82, 137, 274, 425, 666, 1439
BssMI GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
BssNAI GTATAC 1 cut(s) 817
BssT1I CCWWGG 4 cut(s) 82, 137, 274, 666
Bst1107I GTATAC 1 cut(s) 817
Bst2UI CCWGG 3 cut(s) 27, 459, 1440
Bst4CI ACNGT 7 cut(s) 61, 595, 958, 1180, 1387, 1460, 1498
Bst6I CTCTTC 3 cut(s) 297, 990, 1212
BstAFI CTTAAG 1 cut(s) 887
BstC8I GCNNGC 5 cut(s) 44, 243, 500, 901, 1523
BstDEI CTNAG 7 cut(s) 298, 324, 396, 738, 895, 1034, 1263
BstDSI CCRYGG 2 cut(s) 274, 666
BstEII GGTNACC 1 cut(s) 679
BstENI CCTNNNNNAGG 1 cut(s) 1075
BstF5I GGATG 4 cut(s) 406, 613, 830, 1270
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 8 cut(s) 359, 614, 1002, 1153, 1270, 1313, 1369, 1471
BstMAI GTCTC 5 cut(s) 48, 81, 299, 1331, 1523
BstMBI GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
BstMWI GCNNNNNNNGC 2 cut(s) 743, 900
BstNI CCWGG 3 cut(s) 27, 459, 1440
BstNSI RCATGY 1 cut(s) 245
BstPI GGTNACC 1 cut(s) 679
BstSCI CCNGG 4 cut(s) 25, 457, 810, 1438
BstSFI CTRYAG 1 cut(s) 1075
BstUI CGCG 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 727
BstX2I RGATCY 1 cut(s) 356
BstXI CCANNNNNNTGG 2 cut(s) 698, 1446
BstYI RGATCY 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 817
BsuRI GGCC 3 cut(s) 96, 702, 1011
BtgI CCRYGG 2 cut(s) 274, 666
BtgZI GCGATG 2 cut(s) 479, 977
BtsCI GGATG 4 cut(s) 406, 613, 830, 1270
BtsIMutI CAGTG 4 cut(s) 106, 591, 1456, 1494
BveI ACCTGC 1 cut(s) 1588
Cac8I GCNNGC 5 cut(s) 44, 243, 500, 901, 1523
CaiI CAGNNNCTG 1 cut(s) 1346
Cfr13I GGNCC 2 cut(s) 29, 149
Csp6I GTAC 4 cut(s) 119, 489, 1210, 1242
CviAII CATG 8 cut(s) 113, 242, 275, 667, 712, 1084, 1286, 1566
CviQI GTAC 4 cut(s) 119, 489, 1210, 1242
DdeI CTNAG 7 cut(s) 298, 324, 396, 738, 895, 1034, 1263
DpnI GATC 8 cut(s) 358, 613, 1001, 1152, 1269, 1312, 1368, 1470
DpnII GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
DraI TTTAAA 1 cut(s) 1026
EaeI YGGCCR 1 cut(s) 700
Eam1104I CTCTTC 3 cut(s) 297, 990, 1212
EarI CTCTTC 3 cut(s) 297, 990, 1212
Eco130I CCWWGG 4 cut(s) 82, 137, 274, 666
Eco24I GRGCYC 1 cut(s) 330
Eco47I GGWCC 2 cut(s) 29, 149
Eco57I CTGAAG 2 cut(s) 1086, 1368
Eco91I GGTNACC 1 cut(s) 679
EcoNI CCTNNNNNAGG 1 cut(s) 1075
EcoO65I GGTNACC 1 cut(s) 679
EcoRII CCWGG 3 cut(s) 25, 457, 1438
EcoT14I CCWWGG 4 cut(s) 82, 137, 274, 666
EcoT22I ATGCAT 1 cut(s) 1261
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 4 cut(s) 82, 137, 274, 666
Esp3I CGTCTC 1 cut(s) 1523
FaeI CATG 8 cut(s) 116, 245, 278, 670, 715, 1087, 1289, 1569
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 399, 431
FaqI GGGAC 1 cut(s) 1379
FatI CATG 8 cut(s) 112, 241, 274, 666, 711, 1083, 1285, 1565
FauNDI CATATG 1 cut(s) 616
FblI GTMKAC 1 cut(s) 816
FokI GGATG 4 cut(s) 413, 620, 817, 1257
FriOI GRGCYC 1 cut(s) 330
FspBI CTAG 4 cut(s) 5, 261, 267, 1191
GsuI CTGGAG 2 cut(s) 480, 806
HaeIII GGCC 3 cut(s) 96, 702, 1011
HapII CCGG 2 cut(s) 657, 812
Hin1II CATG 8 cut(s) 116, 245, 278, 670, 715, 1087, 1289, 1569
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 3 cut(s) 860, 1194, 1422
HpaII CCGG 2 cut(s) 657, 812
HphI GGTGA 5 cut(s) 673, 800, 1004, 1569, 1595
Hpy166II GTNNAC 5 cut(s) 13, 491, 817, 876, 1577
Hpy188I TCNGA 8 cut(s) 145, 217, 381, 433, 1105, 1147, 1264, 1421
Hpy188III TCNNGA 6 cut(s) 152, 416, 631, 785, 1112, 1160
Hpy8I GTNNAC 5 cut(s) 13, 491, 817, 876, 1577
HpyAV CCTTC 6 cut(s) 218, 404, 746, 848, 1022, 1343
HpyCH4III ACNGT 7 cut(s) 61, 595, 958, 1180, 1387, 1460, 1498
HpyCH4IV ACGT 3 cut(s) 376, 521, 1587
HpyCH4V TGCA 6 cut(s) 46, 557, 1175, 1259, 1279, 1285
HpyF10VI GCNNNNNNNGC 2 cut(s) 743, 900
HpyF3I CTNAG 7 cut(s) 298, 324, 396, 738, 895, 1034, 1263
HpySE526I ACGT 3 cut(s) 376, 521, 1587
Hsp92II CATG 8 cut(s) 116, 245, 278, 670, 715, 1087, 1289, 1569
Kzo9I GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
LmnI GCTCC 1 cut(s) 1162
LweI GCATC 2 cut(s) 1246, 1268
MaeI CTAG 4 cut(s) 5, 261, 267, 1191
MaeII ACGT 3 cut(s) 376, 521, 1587
MaeIII GTNAC 5 cut(s) 160, 391, 631, 679, 772
MalI GATC 8 cut(s) 358, 613, 1001, 1152, 1269, 1312, 1368, 1470
MboI GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
MboII GAAGA 8 cut(s) 314, 350, 419, 518, 521, 732, 1007, 1229
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 2 cut(s) 330, 479
MlyI GAGTC 1 cut(s) 1203
MmeI TCCRAC 1 cut(s) 1288
Mph1103I ATGCAT 1 cut(s) 1261
MseI TTAA 8 cut(s) 344, 368, 447, 621, 888, 984, 1025, 1044
MslI CAYNNNNRTG 5 cut(s) 41, 111, 235, 1284, 1499
MspCI CTTAAG 1 cut(s) 887
MspI CCGG 2 cut(s) 657, 812
MspR9I CCNGG 4 cut(s) 27, 459, 812, 1440
MssI GTTTAAAC 1 cut(s) 1026
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 3 cut(s) 27, 459, 1440
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 2 cut(s) 743, 900
NciI CCSGG 1 cut(s) 812
NcoI CCATGG 2 cut(s) 274, 666
NdeI CATATG 1 cut(s) 616
NdeII GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
NlaIII CATG 8 cut(s) 116, 245, 278, 670, 715, 1087, 1289, 1569
NlaIV GGNNCC 3 cut(s) 280, 976, 1158
NmuCI GTSAC 3 cut(s) 631, 679, 772
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 1 cut(s) 245
OliI CACNNNNGTG 1 cut(s) 235
PaeI GCATGC 1 cut(s) 245
PaqCI CACCTGC 1 cut(s) 1588
PcsI WCGNNNNNNNCGW 1 cut(s) 1529
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 2 cut(s) 860, 1422
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 1571
PfoI TCCNGGA 1 cut(s) 25
PleI GAGTC 1 cut(s) 1202
PmeI GTTTAAAC 1 cut(s) 1026
PpsI GAGTC 1 cut(s) 1202
PshBI ATTAAT 3 cut(s) 344, 447, 984
Psp6I CCWGG 3 cut(s) 25, 457, 1438
PspEI GGTNACC 1 cut(s) 679
PspGI CCWGG 3 cut(s) 25, 457, 1438
PspN4I GGNNCC 3 cut(s) 280, 976, 1158
PspPI GGNCC 2 cut(s) 29, 149
PstNI CAGNNNCTG 1 cut(s) 1346
PsuI RGATCY 1 cut(s) 356
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 4 cut(s) 120, 490, 1211, 1243
RsaNI GTAC 4 cut(s) 119, 489, 1210, 1242
RseI CAYNNNNRTG 5 cut(s) 41, 111, 235, 1284, 1499
SaqAI TTAA 8 cut(s) 344, 368, 447, 621, 888, 984, 1025, 1044
Sau3AI GATC 8 cut(s) 356, 611, 999, 1150, 1267, 1310, 1366, 1468
Sau96I GGNCC 2 cut(s) 29, 149
SchI GAGTC 1 cut(s) 1203
ScrFI CCNGG 4 cut(s) 27, 459, 812, 1440
SduI GDGCHC 2 cut(s) 330, 479
SfaNI GCATC 2 cut(s) 1246, 1268
SfcI CTRYAG 1 cut(s) 1075
SinI GGWCC 2 cut(s) 29, 149
SmiMI CAYNNNNRTG 5 cut(s) 41, 111, 235, 1284, 1499
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
SphI GCATGC 1 cut(s) 245
SsiI CCGC 3 cut(s) 684, 735, 866
SspMI CTAG 4 cut(s) 5, 261, 267, 1191
StyD4I CCNGG 4 cut(s) 25, 457, 810, 1438
StyI CCWWGG 4 cut(s) 82, 137, 274, 666
TaaI ACNGT 7 cut(s) 61, 595, 958, 1180, 1387, 1460, 1498
TaiI ACGT 3 cut(s) 379, 524, 1590
TaqI TCGA 3 cut(s) 405, 1471, 1593
TaqII GACCGA 3 cut(s) 46, 417, 650
TatI WGTACW 3 cut(s) 118, 488, 1209
TfiI GAWTC 2 cut(s) 860, 1422
Tru1I TTAA 8 cut(s) 344, 368, 447, 621, 888, 984, 1025, 1044
Tru9I TTAA 8 cut(s) 344, 368, 447, 621, 888, 984, 1025, 1044
TscAI CASTG 4 cut(s) 113, 598, 1463, 1501
TseFI GTSAC 3 cut(s) 631, 679, 772
Tsp45I GTSAC 3 cut(s) 631, 679, 772
TspDTI ATGAA 5 cut(s) 498, 566, 980, 1230, 1505
TspRI CASTG 4 cut(s) 113, 598, 1463, 1501
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 1571
Vha464I CTTAAG 1 cut(s) 887
VpaK11BI GGWCC 2 cut(s) 29, 149
VspI ATTAAT 3 cut(s) 344, 447, 984
XagI CCTNNNNNAGG 1 cut(s) 1075
XapI RAATTY 2 cut(s) 190, 1610
XceI RCATGY 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 4 cut(s) 89, 135, 673, 1404
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 4 cut(s) 5, 261, 267, 1191
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.