Rw2G037310

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001075
Physical Location & Seq
Reverse (-)
61472844 .. 61478308
5465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G037310.1

Sequence Viewer

Length: 1374 bp
ATGTGTTGCGGATATTCATTACGCTTTGCTATTATTTTCTTGGTAGGCATGGAGTACATCAACCAAGAAATCCTTGGTCAGATGGTCGTCACACAATGTCCTATGGAGCCTAAAACAAATTTCACTTATGAGGTTATATTTTCTGATGAGGAAGGAACACTATGGTGGCATGCCCATAGTGATTGGACTAGAGCTAGTGTCCATGGTGCCATTGTTGTTATGCCCTTAGAAGAGACAGGTTTTCCATTTGCTGAGCCCGATGGAGAAGAATTAATCATATTTGGATCTTGGTATTTAATTGAACGTCTGAACGAGGTGGTTACTGAGGATGTCGAAGAAGGTCTTGACACTCCACGGTCTGATTGCTACACCATTAATGGACAACCTGGAGATTTTGGCTGGTGCTCAAATGAAAGTACACATCGCTGGCAAGTAGAGTATAACAAGACGTATCTTCTTCGTATTGTCAATGCTGTGATGAATGCAGAACTTTACTTTGCCATTGCTGAACATACACTCACTGTTGTTGGTATGGATGGATCATATGTTAAACCCATCGTGACCGATTATATTATGATTTCTGCCGGACAAACCATGGATATTTTGGTCACCGCCAACCAGCCTCTTGGCCGATACTACATGGCTGGAAGACAATTTGACACCGCTAAGTCAGCCGTTGAAGGCTATGACAAATCAAATGTGACAGCAATTCTGGAGTATAAAGGCAATTACTCTCACCCGGTATACCCAATCTTTCCATCCACCCTTCCTGCGTTTGAGGATATAGAATCTGCGGTAAAGTTCACAAGTCGTCTTAAGAGCTTAGCAAGCATAGACCACCCTACAAATGTGCCACAGAATATAACTACCAAAATGTATATAACGGTTGCGATGAATAATGGCACCCTTATTAATGGTGGTGAAGAGATCAAAAGTGGCCTTCTATCAAGTTTAAACAATCTAAGTTGGGTTAATCCATCAACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGATTTTATACTTCAGATTTTCCTGATTTTCCACCAGCATTATTTGACTTCGTATCTGATGATCTGGCTCCTGATAGAACTATTGCAACTGTCAAAGCAACTAGAGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATTGTATTTCAGGGTACTAATTTGCTTGATGCATCTGAGGATCATCCAATGCACTTGCATGGTTATAGCTTTTATGTAGTTGGATCAGGTTTTGGAGATTTTGACAACGAGACAGACCCTGAAGGGTATAATTTGATTGATCCACCAAAGTTAACTACTGTTTCAGTCCCAAAGAAAGGGTGGGTTGCTATCAGATTCAAAGGCAGTAATCCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

457

Amino Acids

51.25

Weight (kDa)

4.53

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 26 - 75 2.7e-11 Multicopper oxidase
Cu-oxidase PF00394 91 - 243 9e-34 Multicopper oxidase
Cu-oxidase_2 PF07731 347 - 456 1.7e-23 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 206, 902
AccI GTMKAC 1 cut(s) 744
AciI CCGC 4 cut(s) 9, 612, 663, 794
AclWI GGATC 5 cut(s) 292, 547, 1203, 1246, 1289
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 2 cut(s) 1014, 1296
AfaI GTAC 4 cut(s) 56, 418, 1139, 1171
AfiI CCNNNNNNNGG 2 cut(s) 1005, 1331
AflII CTTAAG 1 cut(s) 815
AgsI TTSAA 3 cut(s) 302, 680, 1354
AjnI CCWGG 1 cut(s) 385
AleI CACNNNNGTG 1 cut(s) 163
AluBI AGCT 3 cut(s) 194, 822, 1224
AluI AGCT 3 cut(s) 194, 822, 1224
Alw21I GWGCWC 1 cut(s) 407
Alw26I GTCTC 2 cut(s) 227, 1259
AlwI GGATC 5 cut(s) 292, 547, 1203, 1246, 1289
AlwNI CAGNNNCTG 1 cut(s) 1274
AoxI GGCC 2 cut(s) 628, 937
ApoI RAATTY 1 cut(s) 118
AseI ATTAAT 3 cut(s) 272, 375, 912
AsuC2I CCSGG 1 cut(s) 740
AsuHPI GGTGA 3 cut(s) 601, 728, 932
BanI GGYRCC 2 cut(s) 206, 902
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 655
Bbv12I GWGCWC 1 cut(s) 407
BccI CCATC 6 cut(s) 76, 254, 530, 563, 766, 985
BceAI ACGGC 1 cut(s) 659
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 740
BcoDI GTCTC 2 cut(s) 227, 1259
BfaI CTAG 3 cut(s) 189, 195, 1119
BfmI CTRYAG 1 cut(s) 1003
BfrI CTTAAG 1 cut(s) 815
BlpI GCTNAGC 2 cut(s) 252, 823
Bme1390I CCNGG 2 cut(s) 387, 740
BmiI GGNNCC 4 cut(s) 108, 208, 904, 1086
BmrFI CCNGG 2 cut(s) 387, 740
BmsI GCATC 2 cut(s) 1174, 1196
BpiI GAAGAC 1 cut(s) 655
BpmI CTGGAG 2 cut(s) 408, 734
Bpu1102I GCTNAGC 2 cut(s) 252, 823
BpuMI CCSGG 1 cut(s) 740
BsaJI CCNNGG 4 cut(s) 73, 202, 353, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 1005, 1331
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 73, 202, 353, 594
BseGI GGATG 4 cut(s) 334, 541, 758, 1198
BseLI CCNNNNNNNGG 2 cut(s) 1005, 1331
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 243, 315, 1182
BshFI GGCC 2 cut(s) 630, 939
BshNI GGYRCC 2 cut(s) 206, 902
BsiHKAI GWGCWC 1 cut(s) 407
BsiSI CCGG 2 cut(s) 585, 740
BslFI GGGAC 1 cut(s) 1307
BslI CCNNNNNNNGG 2 cut(s) 1005, 1331
BsmAI GTCTC 2 cut(s) 227, 1259
BsmFI GGGAC 1 cut(s) 1307
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 2 cut(s) 630, 939
Bsp1286I GDGCHC 2 cut(s) 258, 407
Bsp143I GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
Bsp1720I GCTNAGC 2 cut(s) 252, 823
Bsp19I CCATGG 2 cut(s) 202, 594
BspACI CCGC 4 cut(s) 9, 612, 663, 794
BspANI GGCC 2 cut(s) 630, 939
BspCNI CTCAG 3 cut(s) 244, 316, 1183
BspLI GGNNCC 4 cut(s) 108, 208, 904, 1086
BspPI GGATC 5 cut(s) 292, 547, 1203, 1246, 1289
BspT107I GGYRCC 2 cut(s) 206, 902
BspTI CTTAAG 1 cut(s) 815
BsrDI GCAATG 1 cut(s) 501
BssECI CCNNGG 4 cut(s) 73, 202, 353, 594
BssMI GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
BssNAI GTATAC 1 cut(s) 745
BssT1I CCWWGG 3 cut(s) 73, 202, 594
Bst1107I GTATAC 1 cut(s) 745
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 5 cut(s) 357, 523, 886, 1108, 1315
Bst6I CTCTTC 3 cut(s) 225, 918, 1140
BstAFI CTTAAG 1 cut(s) 815
BstC8I GCNNGC 3 cut(s) 171, 428, 829
BstDEI CTNAG 7 cut(s) 226, 252, 324, 666, 823, 962, 1191
BstDSI CCRYGG 3 cut(s) 202, 353, 594
BstEII GGTNACC 1 cut(s) 607
BstENI CCTNNNNNAGG 1 cut(s) 1003
BstF5I GGATG 4 cut(s) 334, 541, 758, 1198
BstKTI GATC 7 cut(s) 287, 542, 930, 1081, 1198, 1241, 1297
BstMAI GTCTC 2 cut(s) 227, 1259
BstMBI GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
BstMWI GCNNNNNNNGC 2 cut(s) 671, 828
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 173
BstPI GGTNACC 1 cut(s) 607
BstSCI CCNGG 2 cut(s) 385, 738
BstSFI CTRYAG 1 cut(s) 1003
BstV2I GAAGAC 1 cut(s) 655
BstX2I RGATCY 1 cut(s) 284
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 1 cut(s) 284
BstZ17I GTATAC 1 cut(s) 745
BsuRI GGCC 2 cut(s) 630, 939
BtgI CCRYGG 3 cut(s) 202, 353, 594
BtgZI GCGATG 2 cut(s) 407, 905
BtsCI GGATG 4 cut(s) 334, 541, 758, 1198
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 3 cut(s) 171, 428, 829
CaiI CAGNNNCTG 1 cut(s) 1274
Csp6I GTAC 4 cut(s) 55, 417, 1138, 1170
CviAII CATG 7 cut(s) 49, 170, 203, 595, 640, 1012, 1214
CviQI GTAC 4 cut(s) 55, 417, 1138, 1170
DdeI CTNAG 7 cut(s) 226, 252, 324, 666, 823, 962, 1191
DpnI GATC 7 cut(s) 286, 541, 929, 1080, 1197, 1240, 1296
DpnII GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 225, 918, 1140
EarI CTCTTC 3 cut(s) 225, 918, 1140
Eco130I CCWWGG 3 cut(s) 73, 202, 594
Eco24I GRGCYC 1 cut(s) 258
Eco57I CTGAAG 2 cut(s) 1014, 1296
Eco91I GGTNACC 1 cut(s) 607
EcoNI CCTNNNNNAGG 1 cut(s) 1003
EcoO65I GGTNACC 1 cut(s) 607
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 3 cut(s) 73, 202, 594
EcoT22I ATGCAT 1 cut(s) 1189
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 3 cut(s) 73, 202, 594
FaeI CATG 7 cut(s) 52, 173, 206, 598, 643, 1015, 1217
FalI AAGNNNNNCTT 4 cut(s) 57, 89, 327, 359
FaqI GGGAC 1 cut(s) 1307
FatI CATG 7 cut(s) 48, 169, 202, 594, 639, 1011, 1213
FauNDI CATATG 1 cut(s) 544
FblI GTMKAC 1 cut(s) 744
FokI GGATG 4 cut(s) 341, 548, 745, 1185
FriOI GRGCYC 1 cut(s) 258
FspBI CTAG 3 cut(s) 189, 195, 1119
GsuI CTGGAG 2 cut(s) 408, 734
HaeIII GGCC 2 cut(s) 630, 939
HapII CCGG 2 cut(s) 585, 740
Hin1II CATG 7 cut(s) 52, 173, 206, 598, 643, 1015, 1217
HincII GTYRAC 1 cut(s) 1308
HindII GTYRAC 1 cut(s) 1308
HinfI GANTC 3 cut(s) 788, 1122, 1350
HpaI GTTAAC 1 cut(s) 1308
HpaII CCGG 2 cut(s) 585, 740
HphI GGTGA 3 cut(s) 601, 728, 932
Hpy166II GTNNAC 4 cut(s) 419, 745, 804, 1308
Hpy188I TCNGA 8 cut(s) 81, 145, 309, 361, 1033, 1075, 1192, 1349
Hpy188III TCNNGA 6 cut(s) 344, 559, 713, 1040, 1088, 1367
Hpy8I GTNNAC 4 cut(s) 419, 745, 804, 1308
HpyAV CCTTC 6 cut(s) 146, 332, 674, 776, 950, 1271
HpyCH4III ACNGT 5 cut(s) 357, 523, 886, 1108, 1315
HpyCH4IV ACGT 2 cut(s) 304, 449
HpyCH4V TGCA 5 cut(s) 485, 1103, 1187, 1207, 1213
HpyF10VI GCNNNNNNNGC 2 cut(s) 671, 828
HpyF3I CTNAG 7 cut(s) 226, 252, 324, 666, 823, 962, 1191
HpySE526I ACGT 2 cut(s) 304, 449
Hsp92II CATG 7 cut(s) 52, 173, 206, 598, 643, 1015, 1217
KspAI GTTAAC 1 cut(s) 1308
Kzo9I GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
LmnI GCTCC 2 cut(s) 106, 1090
LweI GCATC 2 cut(s) 1174, 1196
MaeI CTAG 3 cut(s) 189, 195, 1119
MaeII ACGT 2 cut(s) 304, 449
MaeIII GTNAC 5 cut(s) 88, 319, 559, 607, 700
MalI GATC 7 cut(s) 286, 541, 929, 1080, 1197, 1240, 1296
MboI GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
MboII GAAGA 8 cut(s) 242, 278, 347, 446, 449, 660, 935, 1157
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 258, 407
MluCI AATT 9 cut(s) 118, 269, 297, 653, 708, 727, 1155, 1174, 1285
MlyI GAGTC 1 cut(s) 1131
MmeI TCCRAC 1 cut(s) 1216
MnlI CCTC 8 cut(s) 124, 142, 307, 319, 633, 772, 1141, 1186
Mph1103I ATGCAT 1 cut(s) 1189
MseI TTAA 9 cut(s) 272, 296, 375, 549, 816, 912, 953, 972, 1307
MslI CAYNNNNRTG 2 cut(s) 163, 1212
MspCI CTTAAG 1 cut(s) 815
MspI CCGG 2 cut(s) 585, 740
MspR9I CCNGG 2 cut(s) 387, 740
MssI GTTTAAAC 1 cut(s) 954
Mva1269I GAATGC 1 cut(s) 487
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 671, 828
NciI CCSGG 1 cut(s) 740
NcoI CCATGG 2 cut(s) 202, 594
NdeI CATATG 1 cut(s) 544
NdeII GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
NlaIII CATG 7 cut(s) 52, 173, 206, 598, 643, 1015, 1217
NlaIV GGNNCC 4 cut(s) 108, 208, 904, 1086
NmuCI GTSAC 4 cut(s) 88, 559, 607, 700
NsiI ATGCAT 1 cut(s) 1189
NspI RCATGY 1 cut(s) 173
OliI CACNNNNGTG 1 cut(s) 163
PaeI GCATGC 1 cut(s) 173
PctI GAATGC 1 cut(s) 487
PfeI GAWTC 2 cut(s) 788, 1350
PleI GAGTC 1 cut(s) 1130
PmeI GTTTAAAC 1 cut(s) 954
PpsI GAGTC 1 cut(s) 1130
PshBI ATTAAT 3 cut(s) 272, 375, 912
Psp6I CCWGG 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 607
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 4 cut(s) 108, 208, 904, 1086
PstNI CAGNNNCTG 1 cut(s) 1274
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 4 cut(s) 56, 418, 1139, 1171
RsaNI GTAC 4 cut(s) 55, 417, 1138, 1170
RseI CAYNNNNRTG 2 cut(s) 163, 1212
SaqAI TTAA 9 cut(s) 272, 296, 375, 549, 816, 912, 953, 972, 1307
Sau3AI GATC 7 cut(s) 284, 539, 927, 1078, 1195, 1238, 1294
SchI GAGTC 1 cut(s) 1131
ScrFI CCNGG 2 cut(s) 387, 740
SduI GDGCHC 2 cut(s) 258, 407
SfaNI GCATC 2 cut(s) 1174, 1196
SfcI CTRYAG 1 cut(s) 1003
SmiMI CAYNNNNRTG 2 cut(s) 163, 1212
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SphI GCATGC 1 cut(s) 173
Sse9I AATT 9 cut(s) 118, 269, 297, 653, 708, 727, 1155, 1174, 1285
SsiI CCGC 4 cut(s) 9, 612, 663, 794
SspMI CTAG 3 cut(s) 189, 195, 1119
StyD4I CCNGG 2 cut(s) 385, 738
StyI CCWWGG 3 cut(s) 73, 202, 594
TaaI ACNGT 5 cut(s) 357, 523, 886, 1108, 1315
TaiI ACGT 2 cut(s) 307, 452
TaqI TCGA 1 cut(s) 333
TaqII GACCGA 1 cut(s) 578
TasI AATT 9 cut(s) 118, 269, 297, 653, 708, 727, 1155, 1174, 1285
TatI WGTACW 3 cut(s) 54, 416, 1137
TfiI GAWTC 2 cut(s) 788, 1350
Tru1I TTAA 9 cut(s) 272, 296, 375, 549, 816, 912, 953, 972, 1307
Tru9I TTAA 9 cut(s) 272, 296, 375, 549, 816, 912, 953, 972, 1307
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 4 cut(s) 88, 559, 607, 700
Tsp45I GTSAC 4 cut(s) 88, 559, 607, 700
TspDTI ATGAA 5 cut(s) 6, 426, 494, 908, 1158
TspRI CASTG 1 cut(s) 526
Vha464I CTTAAG 1 cut(s) 815
VspI ATTAAT 3 cut(s) 272, 375, 912
XagI CCTNNNNNAGG 1 cut(s) 1003
XapI RAATTY 1 cut(s) 118
XceI RCATGY 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 3 cut(s) 71, 601, 1332
XmiI GTMKAC 1 cut(s) 744
XspI CTAG 3 cut(s) 189, 195, 1119
Zsp2I ATGCAT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.