Rroxscaffold_2G00099650

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001075
Physical Location & Seq
Forward (+)
21395038 .. 21402240
7203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099650.1

Sequence Viewer

Length: 1572 bp
ATGTCAATGTCCAAAACCAAGGAGACTATGGCCTTACTATTCACTGGTAGGCATGGAGTACATCAACCAAGAAATCCTTGGTCAGATGGTCCAGAATATGTCACACAATGTCCTATTGAGCCTAAAACAAATTTCACTTATGAGGTTATATTTTCTGATGAGGAAGGAACACTATGGTGGCACGCCCATAGTGATTGGACTAGAGCTAGTGTCCATGGTGCCATTGTTATTATGCCCTTAGAAGAGACAGGCTTTCCATTTCCTGAGCCCGACGGAGAAGAAATAATCACATTTGGATCTTGGTATTTAATTGAACGTCTGAACGAGGTGGTTACTGAGGATGTCGAAGAAGGTCTTGACACTCCTCGGTCTGCTTGCTACACCATTAATGGACAACCTGGAGATTTTGGCTGGTGCTCAAATGAAAGTACGCATCGCTGGCAAGTAGAGTATAACAAGACGTATCTTTTTCGTATTGTCAATGCTGTGATGAATGCAGAACTCTACTTTGCCATTGCTGAACATACACTCACTGTTGTTGGTATGGATGGATCATATGTTAAACCCATCGTGACCGATTATATTATGATTTCTGCCGGACAAACCATGGATATTTTGGTCACCGCCAACCAGCCTCTTGGCCGATACTACATGGCTGGAAGACAATTTGACACCGCTAAGTCATCTGTTGAAGGCTATGACAAATCAAATGTGACAGCAATTCTGGAGTATAAAGGCAATTACTCTCACTCGGCATACCCAATCTTTCCATCCACCCTTCCTGCGTTTGAGGATTTAGAATCTGCGGTAAAGTTCACAAGTCGTCTTAAGAGCTTAGCAAGCATAGACCACCCTACAAATGTGCCACAGAATATAACTACCAAAATGTATATGACGGTTGCAATGAATAATGGCACCCTTATTAATGGTGGTGAAGAGATCAAAAGTGGCCTTCTATCAAGTTTAAACAATCTAAGTTGGGTTAATCCATCAACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGATTTTATACTTCAGATTTTCCTGATTTTCCACCAGCAATATTTGACTTCGTATCTGATGATCTGGCTCCTGATAGAACTATTGCAACTGTCAAAGCAACTAGAGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATTGTGTTTCAGGGTACTAATTTGCTTGATGCATCTGAGGATCATCCAATGCACTTGCATGGTTATAGCTTTTATGTAGTTGGATCAGGTTTTGGAGATTTTGACAACGAGACAGACCCTGAAGGGTATAATTTGATTGATCCACCGAAGTTAACTACTGTTTCTGTCCCAAAGAAAGGGTGGGTTGCTATCAGATTCAAAGCCAGTAATCCAGGGGTCTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGTATGAACACTGTGATGATAGTGAAAAATGGTGGCACGCCAGAGACGAGCATACGCAGACCACCTCCATACTTGCCTCCATGTAATGGTTCACCTGCCACCGTGTTCGAGCAGTTTCACCACAAATTCAGCAAGACAAGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

523

Amino Acids

58.82

Weight (kDa)

5.09

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 18 - 79 8e-20 Multicopper oxidase
Cu-oxidase PF00394 95 - 247 3e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 357 - 485 2.4e-38 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1528
Acc36I ACCTGC 1 cut(s) 1528
AccB1I GGYRCC 2 cut(s) 218, 914
AccB7I CCANNNNNTGG 1 cut(s) 1511
AciI CCGC 3 cut(s) 624, 675, 806
AclWI GGATC 6 cut(s) 304, 559, 1215, 1258, 1301, 1416
AcoI YGGCCR 1 cut(s) 640
AcsI RAATTY 2 cut(s) 130, 1550
AcuI CTGAAG 2 cut(s) 1026, 1308
AfaI GTAC 4 cut(s) 60, 430, 1151, 1183
AfiI CCNNNNNNNGG 3 cut(s) 1017, 1343, 1511
AflII CTTAAG 1 cut(s) 827
AgsI TTSAA 3 cut(s) 314, 692, 1366
AjnI CCWGG 2 cut(s) 397, 1378
AleI CACNNNNGTG 1 cut(s) 175
AluBI AGCT 3 cut(s) 206, 834, 1236
AluI AGCT 3 cut(s) 206, 834, 1236
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 4 cut(s) 17, 239, 1271, 1463
AlwI GGATC 6 cut(s) 304, 559, 1215, 1258, 1301, 1416
AlwNI CAGNNNCTG 1 cut(s) 1286
AoxI GGCC 3 cut(s) 30, 640, 949
ApoI RAATTY 2 cut(s) 130, 1550
AseI ATTAAT 2 cut(s) 387, 924
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 4 cut(s) 613, 944, 1509, 1535
AvaII GGWCC 1 cut(s) 89
BanI GGYRCC 2 cut(s) 218, 914
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 667
Bbv12I GWGCWC 1 cut(s) 419
BccI CCATC 5 cut(s) 80, 542, 575, 778, 997
BciT130I CCWGG 2 cut(s) 399, 1380
BcoDI GTCTC 4 cut(s) 17, 239, 1271, 1463
BfaI CTAG 3 cut(s) 201, 207, 1131
BfmI CTRYAG 1 cut(s) 1015
BfrI CTTAAG 1 cut(s) 827
BfuAI ACCTGC 1 cut(s) 1528
BlpI GCTNAGC 1 cut(s) 835
Bme1390I CCNGG 2 cut(s) 399, 1380
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 3 cut(s) 220, 916, 1098
BmrFI CCNGG 2 cut(s) 399, 1380
BmsI GCATC 3 cut(s) 442, 1186, 1208
BpiI GAAGAC 1 cut(s) 667
BpmI CTGGAG 2 cut(s) 420, 746
Bpu10I CCTNAGC 1 cut(s) 264
Bpu1102I GCTNAGC 1 cut(s) 835
BsaJI CCNNGG 6 cut(s) 18, 77, 214, 365, 606, 1379
Bsc4I CCNNNNNNNGG 3 cut(s) 1017, 1343, 1511
Bse1I ACTGG 2 cut(s) 49, 1371
Bse3DI GCAATG 2 cut(s) 513, 909
BseBI CCWGG 2 cut(s) 399, 1380
BseDI CCNNGG 6 cut(s) 18, 77, 214, 365, 606, 1379
BseGI GGATG 4 cut(s) 346, 553, 770, 1210
BseLI CCNNNNNNNGG 3 cut(s) 1017, 1343, 1511
BseMI GCAATG 2 cut(s) 513, 909
BseMII CTCAG 3 cut(s) 255, 327, 1194
BseNI ACTGG 2 cut(s) 49, 1371
BseRI GAGGAG 1 cut(s) 354
BshFI GGCC 3 cut(s) 32, 642, 951
BshNI GGYRCC 2 cut(s) 218, 914
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 1 cut(s) 597
BslFI GGGAC 1 cut(s) 1319
BslI CCNNNNNNNGG 3 cut(s) 1017, 1343, 1511
BsmAI GTCTC 4 cut(s) 17, 239, 1271, 1463
BsmBI CGTCTC 1 cut(s) 1463
BsmFI GGGAC 1 cut(s) 1319
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 3 cut(s) 32, 642, 951
Bsp1286I GDGCHC 2 cut(s) 270, 419
Bsp143I GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
Bsp1720I GCTNAGC 1 cut(s) 835
Bsp19I CCATGG 2 cut(s) 214, 606
BspACI CCGC 3 cut(s) 624, 675, 806
BspANI GGCC 3 cut(s) 32, 642, 951
BspCNI CTCAG 3 cut(s) 256, 328, 1195
BspLI GGNNCC 3 cut(s) 220, 916, 1098
BspMI ACCTGC 1 cut(s) 1528
BspPI GGATC 6 cut(s) 304, 559, 1215, 1258, 1301, 1416
BspT107I GGYRCC 2 cut(s) 218, 914
BspTI CTTAAG 1 cut(s) 827
BsrDI GCAATG 2 cut(s) 513, 909
BsrI ACTGG 2 cut(s) 49, 1371
BssECI CCNNGG 6 cut(s) 18, 77, 214, 365, 606, 1379
BssMI GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
BssT1I CCWWGG 4 cut(s) 18, 77, 214, 606
Bst2UI CCWGG 2 cut(s) 399, 1380
Bst4CI ACNGT 7 cut(s) 535, 898, 1120, 1327, 1400, 1438, 1528
Bst6I CTCTTC 3 cut(s) 237, 930, 1152
BstAFI CTTAAG 1 cut(s) 827
BstC8I GCNNGC 5 cut(s) 183, 376, 440, 841, 1463
BstDEI CTNAG 7 cut(s) 238, 264, 336, 678, 835, 974, 1203
BstDSI CCRYGG 2 cut(s) 214, 606
BstEII GGTNACC 1 cut(s) 619
BstENI CCTNNNNNAGG 1 cut(s) 1015
BstF5I GGATG 4 cut(s) 346, 553, 770, 1210
BstKTI GATC 8 cut(s) 299, 554, 942, 1093, 1210, 1253, 1309, 1411
BstMAI GTCTC 4 cut(s) 17, 239, 1271, 1463
BstMBI GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
BstMWI GCNNNNNNNGC 2 cut(s) 439, 840
BstNI CCWGG 2 cut(s) 399, 1380
BstPI GGTNACC 1 cut(s) 619
BstSCI CCNGG 2 cut(s) 397, 1378
BstSFI CTRYAG 1 cut(s) 1015
BstV2I GAAGAC 1 cut(s) 667
BstX2I RGATCY 1 cut(s) 296
BstXI CCANNNNNNTGG 2 cut(s) 638, 1386
BstYI RGATCY 1 cut(s) 296
BsuRI GGCC 3 cut(s) 32, 642, 951
BtgI CCRYGG 2 cut(s) 214, 606
BtgZI GCGATG 1 cut(s) 419
BtsCI GGATG 4 cut(s) 346, 553, 770, 1210
BtsIMutI CAGTG 4 cut(s) 42, 531, 1396, 1434
BveI ACCTGC 1 cut(s) 1528
Cac8I GCNNGC 5 cut(s) 183, 376, 440, 841, 1463
CaiI CAGNNNCTG 1 cut(s) 1286
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 4 cut(s) 59, 429, 1150, 1182
CviAII CATG 7 cut(s) 53, 215, 607, 652, 1024, 1226, 1506
CviQI GTAC 4 cut(s) 59, 429, 1150, 1182
DdeI CTNAG 7 cut(s) 238, 264, 336, 678, 835, 974, 1203
DpnI GATC 8 cut(s) 298, 553, 941, 1092, 1209, 1252, 1308, 1410
DpnII GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
DraI TTTAAA 1 cut(s) 966
EaeI YGGCCR 1 cut(s) 640
Eam1104I CTCTTC 3 cut(s) 237, 930, 1152
EarI CTCTTC 3 cut(s) 237, 930, 1152
Eco130I CCWWGG 4 cut(s) 18, 77, 214, 606
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 1026, 1308
Eco91I GGTNACC 1 cut(s) 619
EcoNI CCTNNNNNAGG 1 cut(s) 1015
EcoO65I GGTNACC 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 397, 1378
EcoT14I CCWWGG 4 cut(s) 18, 77, 214, 606
EcoT22I ATGCAT 1 cut(s) 1201
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 4 cut(s) 18, 77, 214, 606
Esp3I CGTCTC 1 cut(s) 1463
FaeI CATG 7 cut(s) 56, 218, 610, 655, 1027, 1229, 1509
FalI AAGNNNNNCTT 4 cut(s) 61, 93, 339, 371
FaqI GGGAC 1 cut(s) 1319
FatI CATG 7 cut(s) 52, 214, 606, 651, 1023, 1225, 1505
FauNDI CATATG 1 cut(s) 556
FokI GGATG 4 cut(s) 353, 560, 757, 1197
FriOI GRGCYC 1 cut(s) 270
FspBI CTAG 3 cut(s) 201, 207, 1131
GsuI CTGGAG 2 cut(s) 420, 746
HaeIII GGCC 3 cut(s) 32, 642, 951
HapII CCGG 1 cut(s) 597
Hin1II CATG 7 cut(s) 56, 218, 610, 655, 1027, 1229, 1509
HincII GTYRAC 1 cut(s) 1320
HindII GTYRAC 1 cut(s) 1320
HinfI GANTC 3 cut(s) 800, 1134, 1362
HpaI GTTAAC 1 cut(s) 1320
HpaII CCGG 1 cut(s) 597
HphI GGTGA 4 cut(s) 613, 944, 1509, 1535
Hpy166II GTNNAC 3 cut(s) 816, 1320, 1517
Hpy188I TCNGA 7 cut(s) 85, 157, 321, 1045, 1087, 1204, 1361
Hpy188III TCNNGA 7 cut(s) 92, 263, 356, 571, 725, 1052, 1100
Hpy8I GTNNAC 3 cut(s) 816, 1320, 1517
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 6 cut(s) 158, 344, 686, 788, 962, 1283
HpyCH4III ACNGT 7 cut(s) 535, 898, 1120, 1327, 1400, 1438, 1528
HpyCH4IV ACGT 2 cut(s) 316, 461
HpyCH4V TGCA 6 cut(s) 497, 902, 1115, 1199, 1219, 1225
HpyF10VI GCNNNNNNNGC 2 cut(s) 439, 840
HpyF3I CTNAG 7 cut(s) 238, 264, 336, 678, 835, 974, 1203
HpySE526I ACGT 2 cut(s) 316, 461
Hsp92II CATG 7 cut(s) 56, 218, 610, 655, 1027, 1229, 1509
KspAI GTTAAC 1 cut(s) 1320
Kzo9I GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
LmnI GCTCC 1 cut(s) 1102
LweI GCATC 3 cut(s) 442, 1186, 1208
MaeI CTAG 3 cut(s) 201, 207, 1131
MaeII ACGT 2 cut(s) 316, 461
MaeIII GTNAC 5 cut(s) 100, 331, 571, 619, 712
MalI GATC 8 cut(s) 298, 553, 941, 1092, 1209, 1252, 1308, 1410
MboI GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
MboII GAAGA 6 cut(s) 254, 290, 359, 672, 947, 1169
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 2 cut(s) 270, 419
MluCI AATT 9 cut(s) 130, 309, 665, 720, 739, 1167, 1186, 1297, 1550
MlyI GAGTC 1 cut(s) 1143
MmeI TCCRAC 1 cut(s) 1228
Mph1103I ATGCAT 1 cut(s) 1201
MseI TTAA 8 cut(s) 308, 387, 561, 828, 924, 965, 984, 1319
MslI CAYNNNNRTG 3 cut(s) 175, 1224, 1439
MspCI CTTAAG 1 cut(s) 827
MspI CCGG 1 cut(s) 597
MspR9I CCNGG 2 cut(s) 399, 1380
MssI GTTTAAAC 1 cut(s) 966
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 2 cut(s) 399, 1380
MwoI GCNNNNNNNGC 2 cut(s) 439, 840
NcoI CCATGG 2 cut(s) 214, 606
NdeI CATATG 1 cut(s) 556
NdeII GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
NlaIII CATG 7 cut(s) 56, 218, 610, 655, 1027, 1229, 1509
NlaIV GGNNCC 3 cut(s) 220, 916, 1098
NmeAIII GCCGAG 1 cut(s) 731
NmuCI GTSAC 4 cut(s) 100, 571, 619, 712
NsiI ATGCAT 1 cut(s) 1201
OliI CACNNNNGTG 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 1528
PcsI WCGNNNNNNNCGW 1 cut(s) 1469
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 2 cut(s) 800, 1362
PflMI CCANNNNNTGG 1 cut(s) 1511
PleI GAGTC 1 cut(s) 1142
PmeI GTTTAAAC 1 cut(s) 966
PpsI GAGTC 1 cut(s) 1142
PshBI ATTAAT 2 cut(s) 387, 924
Psp6I CCWGG 2 cut(s) 397, 1378
PspEI GGTNACC 1 cut(s) 619
PspGI CCWGG 2 cut(s) 397, 1378
PspN4I GGNNCC 3 cut(s) 220, 916, 1098
PspPI GGNCC 1 cut(s) 89
PstNI CAGNNNCTG 1 cut(s) 1286
PsuI RGATCY 1 cut(s) 296
RsaI GTAC 4 cut(s) 60, 430, 1151, 1183
RsaNI GTAC 4 cut(s) 59, 429, 1150, 1182
RseI CAYNNNNRTG 3 cut(s) 175, 1224, 1439
SaqAI TTAA 8 cut(s) 308, 387, 561, 828, 924, 965, 984, 1319
Sau3AI GATC 8 cut(s) 296, 551, 939, 1090, 1207, 1250, 1306, 1408
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 1 cut(s) 1143
ScrFI CCNGG 2 cut(s) 399, 1380
SduI GDGCHC 2 cut(s) 270, 419
SfaNI GCATC 3 cut(s) 442, 1186, 1208
SfcI CTRYAG 1 cut(s) 1015
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 3 cut(s) 175, 1224, 1439
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 9 cut(s) 130, 309, 665, 720, 739, 1167, 1186, 1297, 1550
SsiI CCGC 3 cut(s) 624, 675, 806
SspI AATATT 1 cut(s) 1071
SspMI CTAG 3 cut(s) 201, 207, 1131
StyD4I CCNGG 2 cut(s) 397, 1378
StyI CCWWGG 4 cut(s) 18, 77, 214, 606
TaaI ACNGT 7 cut(s) 535, 898, 1120, 1327, 1400, 1438, 1528
TaiI ACGT 2 cut(s) 319, 464
TaqI TCGA 3 cut(s) 345, 1411, 1533
TaqII GACCGA 2 cut(s) 357, 590
TasI AATT 9 cut(s) 130, 309, 665, 720, 739, 1167, 1186, 1297, 1550
TatI WGTACW 2 cut(s) 58, 1149
TfiI GAWTC 2 cut(s) 800, 1362
Tru1I TTAA 8 cut(s) 308, 387, 561, 828, 924, 965, 984, 1319
Tru9I TTAA 8 cut(s) 308, 387, 561, 828, 924, 965, 984, 1319
TscAI CASTG 4 cut(s) 49, 538, 1403, 1441
TseFI GTSAC 4 cut(s) 100, 571, 619, 712
Tsp45I GTSAC 4 cut(s) 100, 571, 619, 712
TspDTI ATGAA 5 cut(s) 438, 506, 920, 1170, 1445
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 4 cut(s) 49, 538, 1403, 1441
Van91I CCANNNNNTGG 1 cut(s) 1511
Vha464I CTTAAG 1 cut(s) 827
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 2 cut(s) 387, 924
XagI CCTNNNNNAGG 1 cut(s) 1015
XapI RAATTY 2 cut(s) 130, 1550
XcmI CCANNNNNNNNNTGG 4 cut(s) 25, 75, 613, 1344
XspI CTAG 3 cut(s) 201, 207, 1131
Zsp2I ATGCAT 1 cut(s) 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.