RchiOBHm_Chr6g0273731

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001075
Physical Location & Seq
Reverse (-)
34703219 .. 34709270
6052 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24556

Sequence Viewer

Length: 1815 bp
ATGCATATATATAGAACTAGAACAACCAGAACAACTAGTTTCCGTGTGAGGATGGGTGTGCTCCAATGGTTGCTGGGTTCTCTGCTTCTATGCAGTGCGCTTCTTTGCATAGTTGAAGGAAATGTTCACTACTATGACTTTGTACTCAGGGAGGCCAACTTTACAAGGTTGTGTCAGACTAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGTCATTCGTGTACAAAAAGGTGATACTGTTTATGTCAATGTCCAAAATCAAGGAGCCTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCAAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACGCAGTGTCCAATTGAACCTGGATCAAATTTCACTTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGATTGGACCAGAGCTAGTGTCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACAGGTTTTCCATTTCCCGAGCCAGATGGAGAAGAAATAGTTGTACTTGGTTCTTGGTATTATATTGATAATCTGAATGAGGTGGTTGCTAAGGACGTCGAAGAAGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTCAGCCTATGCTCAAATGACAGTACGTATCGCTGGCAAGTGGAGTATGGCAAGACGTACCTTCTTCGCCTGGTTAGTGCAGTTATGAATGCAGAACTCTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTAAAACCGATTGTGACCAATAATATCATGATTTCCCCCGGACAAACCATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGTGTTAGGCCAGATGATCAAGACTATGACAAGTCAAATGTTACAGCAATTCTGGAGTATAAAGGCAATTACACAACCCCTGCAGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCGATAGAGTTTACAGACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGATGATGTGACAGTTGCTCTTCTTGCAAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGAAAATTTGCCTGATAATACTGAGGTTACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATAGTGTTTCAGGGTACTAATACAATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGATATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGACAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTATTTCAGTCCCAATGAAAGGATGGGTTGCTATCAGGTTCAAAGCAAGTAATCCTGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGAGGCACAGCGGAGAGGTGCATGCGAGAACCTCCTCCATACATGCCTCCCTGCACTGATTCACCTACCATCAGGTTGCGACCTTCCCACAAATTCAGCAGGACGGCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

604

Amino Acids

68.18

Weight (kDa)

5.18

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 51 - 163 4.9e-40 Multicopper oxidase
Cu-oxidase PF00394 177 - 329 6.1e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 442 - 567 4.6e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 591
Acc36I ACCTGC 1 cut(s) 1060
AccB1I GGYRCC 1 cut(s) 464
AciI CCGC 2 cut(s) 1145, 1712
AclWI GGATC 5 cut(s) 379, 1504, 1523, 1547, 1662
AcsI RAATTY 4 cut(s) 376, 1339, 1510, 1793
AcyI GRCGYC 1 cut(s) 588
AdeI CACNNNGTG 1 cut(s) 354
AfaI GTAC 9 cut(s) 144, 231, 537, 676, 710, 1024, 1139, 1397, 1429
AfiI CCNNNNNNNGG 2 cut(s) 1266, 1589
AgsI TTSAA 3 cut(s) 116, 365, 1612
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 5 cut(s) 211, 367, 643, 720, 1624
AleI CACNNNNGTG 1 cut(s) 421
AluBI AGCT 7 cut(s) 452, 872, 1083, 1227, 1482, 1539, 1680
AluI AGCT 7 cut(s) 452, 872, 1083, 1227, 1482, 1539, 1680
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 2 cut(s) 485, 885
AlwI GGATC 5 cut(s) 379, 1504, 1523, 1547, 1662
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 4 cut(s) 153, 215, 280, 926
ApeKI GCWGC 2 cut(s) 902, 1001
ApoI RAATTY 4 cut(s) 376, 1339, 1510, 1793
AseI ATTAAT 1 cut(s) 1440
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 3 cut(s) 215, 335, 444
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 3 cut(s) 251, 1129, 1755
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 2 cut(s) 335, 444
BanI GGYRCC 1 cut(s) 464
BarI GAAGNNNNNNTAC 2 cut(s) 1015, 1047
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 889, 1013
BccI CCATC 6 cut(s) 46, 326, 512, 788, 1587, 1778
BciT130I CCWGG 5 cut(s) 213, 369, 645, 722, 1626
BclI TGATCA 1 cut(s) 934
BcnI CCSGG 1 cut(s) 843
BcoDI GTCTC 2 cut(s) 485, 885
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 4 cut(s) 18, 36, 453, 906
BfmI CTRYAG 3 cut(s) 999, 1242, 1264
BfuAI ACCTGC 1 cut(s) 1060
BisI GCNGC 2 cut(s) 903, 1002
BlpI GCTNAGC 1 cut(s) 1084
BlsI GCNGC 2 cut(s) 904, 1003
Bme1390I CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1626
Bme18I GGWCC 2 cut(s) 335, 444
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 215, 335, 444
BmiI GGNNCC 3 cut(s) 274, 320, 466
BmrFI CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1626
BmsI GCATC 2 cut(s) 316, 1454
BpmI CTGGAG 1 cut(s) 992
Bpu10I CCTNAGC 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 1084
BpuMI CCSGG 1 cut(s) 843
BsaAI YACGTR 1 cut(s) 678
BsaHI GRCGYC 1 cut(s) 588
BsaJI CCNNGG 8 cut(s) 212, 314, 323, 460, 841, 852, 1377, 1625
BsaXI ACNNNNNCTCC 2 cut(s) 789, 819
Bsc4I CCNNNNNNNGG 2 cut(s) 1266, 1589
Bse118I RCCGGY 1 cut(s) 1114
Bse1I ACTGG 3 cut(s) 218, 299, 877
BseBI CCWGG 5 cut(s) 213, 369, 645, 722, 1626
BseDI CCNNGG 8 cut(s) 212, 314, 323, 460, 841, 852, 1377, 1625
BseGI GGATG 4 cut(s) 57, 799, 1456, 1598
BseLI CCNNNNNNNGG 2 cut(s) 1266, 1589
BseMII CTCAG 4 cut(s) 160, 330, 1305, 1347
BseNI ACTGG 3 cut(s) 218, 299, 877
BseRI GAGGAG 2 cut(s) 600, 1725
BseXI GCAGC 2 cut(s) 889, 1013
BseYI CCCAGC 2 cut(s) 73, 1227
BsgI GTGCAG 2 cut(s) 750, 1738
BshFI GGCC 4 cut(s) 155, 217, 282, 928
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 843, 1115
BslFI GGGAC 1 cut(s) 1565
BslI CCNNNNNNNGG 2 cut(s) 1266, 1589
BsmAI GTCTC 2 cut(s) 485, 885
BsmFI GGGAC 1 cut(s) 1565
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 4 cut(s) 155, 217, 282, 928
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 63
Bsp1407I TGTACA 2 cut(s) 229, 1137
Bsp143I GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
Bsp1720I GCTNAGC 1 cut(s) 1084
Bsp19I CCATGG 2 cut(s) 460, 852
BspACI CCGC 2 cut(s) 1145, 1712
BspANI GGCC 4 cut(s) 155, 217, 282, 928
BspCNI CTCAG 4 cut(s) 159, 331, 1304, 1348
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 3 cut(s) 274, 320, 466
BspMAI CTGCAG 1 cut(s) 1003
BspMI ACCTGC 1 cut(s) 1060
BspPI GGATC 5 cut(s) 379, 1504, 1523, 1547, 1662
BspQI GCTCTTC 1 cut(s) 1206
BspT107I GGYRCC 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 1114
BsrGI TGTACA 2 cut(s) 229, 1137
BsrI ACTGG 3 cut(s) 218, 299, 877
BssAI RCCGGY 1 cut(s) 1114
BssECI CCNNGG 8 cut(s) 212, 314, 323, 460, 841, 852, 1377, 1625
BssMI GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
BssNI GRCGYC 1 cut(s) 588
BssT1I CCWWGG 5 cut(s) 314, 323, 460, 852, 1377
Bst2UI CCWGG 5 cut(s) 213, 369, 645, 722, 1626
Bst4CI ACNGT 7 cut(s) 206, 247, 674, 781, 1195, 1366, 1646
Bst6I CTCTTC 2 cut(s) 1206, 1398
BstACI GRCGYC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 304
BstAUI TGTACA 2 cut(s) 229, 1137
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 5 cut(s) 188, 429, 686, 1090, 1724
BstDEI CTNAG 7 cut(s) 146, 180, 339, 582, 1084, 1291, 1356
BstDSI CCRYGG 2 cut(s) 460, 852
BstENI CCTNNNNNAGG 1 cut(s) 1264
BstF5I GGATG 4 cut(s) 57, 799, 1456, 1598
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 6 cut(s) 374, 937, 1499, 1531, 1555, 1657
BstMAI GTCTC 2 cut(s) 485, 885
BstMBI GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
BstMWI GCNNNNNNNGC 4 cut(s) 304, 621, 1089, 1205
BstNI CCWGG 5 cut(s) 213, 369, 645, 722, 1626
BstNSI RCATGY 4 cut(s) 190, 431, 1726, 1747
BstSCI CCNGG 6 cut(s) 211, 367, 643, 720, 841, 1624
BstSFI CTRYAG 3 cut(s) 999, 1242, 1264
BstSNI TACGTA 1 cut(s) 678
BstV1I GCAGC 2 cut(s) 889, 1013
BstX2I RGATCY 1 cut(s) 1528
BstXI CCANNNNNNTGG 1 cut(s) 884
BstYI RGATCY 1 cut(s) 1528
BsuRI GGCC 4 cut(s) 155, 217, 282, 928
BtgI CCRYGG 2 cut(s) 460, 852
BtsCI GGATG 4 cut(s) 57, 799, 1456, 1598
BtsI GCAGTG 2 cut(s) 100, 359
BtsIMutI CAGTG 6 cut(s) 100, 292, 359, 777, 1642, 1755
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 5 cut(s) 188, 429, 686, 1090, 1724
CciI TCATGA 1 cut(s) 831
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 1114
Cfr13I GGNCC 3 cut(s) 215, 335, 444
Csp6I GTAC 9 cut(s) 143, 230, 536, 675, 709, 1023, 1138, 1396, 1428
CviQI GTAC 9 cut(s) 143, 230, 536, 675, 709, 1023, 1138, 1396, 1428
DdeI CTNAG 7 cut(s) 146, 180, 339, 582, 1084, 1291, 1356
DpnI GATC 6 cut(s) 373, 936, 1498, 1530, 1554, 1656
DpnII GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
DraI TTTAAA 1 cut(s) 1215
DraIII CACNNNGTG 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 1206, 1398
EarI CTCTTC 2 cut(s) 1206, 1398
Eco105I TACGTA 1 cut(s) 678
Eco130I CCWWGG 5 cut(s) 314, 323, 460, 852, 1377
Eco47I GGWCC 2 cut(s) 335, 444
Eco88I CYCGRG 1 cut(s) 509
EcoNI CCTNNNNNAGG 1 cut(s) 1264
EcoRII CCWGG 5 cut(s) 211, 367, 643, 720, 1624
EcoT14I CCWWGG 5 cut(s) 314, 323, 460, 852, 1377
EcoT22I ATGCAT 2 cut(s) 6, 1447
ErhI CCWWGG 5 cut(s) 314, 323, 460, 852, 1377
FalI AAGNNNNNCTT 4 cut(s) 307, 339, 402, 434
FaqI GGGAC 1 cut(s) 1565
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 2 cut(s) 903, 1002
FokI GGATG 4 cut(s) 64, 806, 1443, 1605
Fsp4HI GCNGC 2 cut(s) 903, 1002
FspBI CTAG 4 cut(s) 18, 36, 453, 906
GlaI GCGC 1 cut(s) 99
GluI GCNGC 2 cut(s) 903, 1002
GsaI CCCAGC 2 cut(s) 77, 1231
GsuI CTGGAG 1 cut(s) 992
HaeIII GGCC 4 cut(s) 155, 217, 282, 928
HapII CCGG 2 cut(s) 843, 1115
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 588
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 2 cut(s) 1453, 1760
HpaII CCGG 2 cut(s) 843, 1115
HphI GGTGA 3 cut(s) 251, 1129, 1755
Hpy166II GTNNAC 3 cut(s) 127, 230, 1065
Hpy188III TCNNGA 6 cut(s) 338, 509, 832, 938, 971, 1078
Hpy8I GTNNAC 3 cut(s) 127, 230, 1065
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 6 cut(s) 110, 404, 590, 722, 1041, 1794
HpyCH4III ACNGT 7 cut(s) 206, 247, 674, 781, 1195, 1366, 1646
HpyCH4IV ACGT 3 cut(s) 588, 677, 707
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 621, 1089, 1205
HpyF3I CTNAG 7 cut(s) 146, 180, 339, 582, 1084, 1291, 1356
HpySE526I ACGT 3 cut(s) 588, 677, 707
Hsp92I GRCGYC 1 cut(s) 588
HspAI GCGC 1 cut(s) 98
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 3 cut(s) 66, 272, 797
Lsp1109I GCAGC 2 cut(s) 889, 1013
LweI GCATC 2 cut(s) 316, 1454
MaeI CTAG 4 cut(s) 18, 36, 453, 906
MaeII ACGT 3 cut(s) 588, 677, 707
MaeIII GTNAC 6 cut(s) 346, 817, 865, 958, 1189, 1360
MalI GATC 6 cut(s) 373, 936, 1498, 1530, 1554, 1656
MboI GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
MboII GAAGA 7 cut(s) 536, 605, 707, 1193, 1415, 1573, 1708
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 1 cut(s) 1528
MhlI GDGCHC 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 1262, 1474
Mph1103I ATGCAT 2 cut(s) 6, 1447
MseI TTAA 3 cut(s) 1214, 1233, 1440
MslI CAYNNNNRTG 4 cut(s) 132, 297, 421, 1470
MspA1I CMGCKG 1 cut(s) 1712
MspI CCGG 2 cut(s) 843, 1115
MspR9I CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1626
MssI GTTTAAAC 1 cut(s) 1215
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 5 cut(s) 213, 369, 645, 722, 1626
MwoI GCNNNNNNNGC 4 cut(s) 304, 621, 1089, 1205
NciI CCSGG 1 cut(s) 843
NcoI CCATGG 2 cut(s) 460, 852
NdeI CATATG 1 cut(s) 802
NdeII GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
NlaIV GGNNCC 3 cut(s) 274, 320, 466
NmuCI GTSAC 4 cut(s) 346, 817, 865, 1189
NsiI ATGCAT 2 cut(s) 6, 1447
NspI RCATGY 4 cut(s) 190, 431, 1726, 1747
OliI CACNNNNGTG 1 cut(s) 421
PaeI GCATGC 3 cut(s) 190, 431, 1726
PagI TCATGA 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 1206
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 2 cut(s) 1453, 1760
PkrI GCNGC 2 cut(s) 904, 1003
PmeI GTTTAAAC 1 cut(s) 1215
Ppu21I YACGTR 1 cut(s) 678
PshBI ATTAAT 1 cut(s) 1440
Psp6I CCWGG 5 cut(s) 211, 367, 643, 720, 1624
PspFI CCCAGC 2 cut(s) 73, 1227
PspGI CCWGG 5 cut(s) 211, 367, 643, 720, 1624
PspN4I GGNNCC 3 cut(s) 274, 320, 466
PspPI GGNCC 3 cut(s) 215, 335, 444
PstI CTGCAG 1 cut(s) 1003
PsuI RGATCY 1 cut(s) 1528
RsaI GTAC 9 cut(s) 144, 231, 537, 676, 710, 1024, 1139, 1397, 1429
RsaNI GTAC 9 cut(s) 143, 230, 536, 675, 709, 1023, 1138, 1396, 1428
RseI CAYNNNNRTG 4 cut(s) 132, 297, 421, 1470
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 3 cut(s) 1214, 1233, 1440
SatI GCNGC 2 cut(s) 903, 1002
Sau3AI GATC 6 cut(s) 371, 934, 1496, 1528, 1552, 1654
Sau96I GGNCC 3 cut(s) 215, 335, 444
ScrFI CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1626
SduI GDGCHC 1 cut(s) 63
SfaNI GCATC 2 cut(s) 316, 1454
SfcI CTRYAG 3 cut(s) 999, 1242, 1264
SinI GGWCC 2 cut(s) 335, 444
SmiMI CAYNNNNRTG 4 cut(s) 132, 297, 421, 1470
SnaBI TACGTA 1 cut(s) 678
SpeI ACTAGT 1 cut(s) 35
SphI GCATGC 3 cut(s) 190, 431, 1726
SsiI CCGC 2 cut(s) 1145, 1712
SspMI CTAG 4 cut(s) 18, 36, 453, 906
StyD4I CCNGG 6 cut(s) 211, 367, 643, 720, 841, 1624
StyI CCWWGG 5 cut(s) 314, 323, 460, 852, 1377
TaaI ACNGT 7 cut(s) 206, 247, 674, 781, 1195, 1366, 1646
TaiI ACGT 3 cut(s) 591, 680, 710
TaqI TCGA 2 cut(s) 591, 1657
TatI WGTACW 5 cut(s) 142, 229, 535, 1137, 1395
TfiI GAWTC 2 cut(s) 1453, 1760
Tru1I TTAA 3 cut(s) 1214, 1233, 1440
Tru9I TTAA 3 cut(s) 1214, 1233, 1440
TscAI CASTG 6 cut(s) 100, 299, 359, 784, 1649, 1762
TseFI GTSAC 4 cut(s) 346, 817, 865, 1189
TseI GCWGC 2 cut(s) 902, 1001
Tsp45I GTSAC 4 cut(s) 346, 817, 865, 1189
TspDTI ATGAA 6 cut(s) 647, 752, 1169, 1350, 1416, 1601
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 6 cut(s) 100, 299, 359, 784, 1649, 1762
VpaK11BI GGWCC 2 cut(s) 335, 444
VspI ATTAAT 1 cut(s) 1440
XagI CCTNNNNNAGG 1 cut(s) 1264
XapI RAATTY 4 cut(s) 376, 1339, 1510, 1793
XceI RCATGY 4 cut(s) 190, 431, 1726, 1747
XcmI CCANNNNNNNNNTGG 3 cut(s) 321, 859, 1590
XspI CTAG 4 cut(s) 18, 36, 453, 906
ZraI GACGTC 1 cut(s) 589
Zsp2I ATGCAT 2 cut(s) 6, 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.