Rh6AG191900

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001075
Physical Location & Seq
Reverse (-)
34324152 .. 34328850
4699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG191900.1

Sequence Viewer

Length: 1761 bp
ATGGGTGTGCTCCAATGGTTGCTGGGTTCTCTGCTTCTATGCAGTGCACTTCTTTGCATAGTTGAAGGAAATGTTCACTACTATGACTTTGTACTCAAGGAGGCCAACTTTACAAGGTTATGTCAGACCAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGTCATTCGTGTACAAAAAGGTGATACTGTTTATGTCAATGTCCAGAATCAAGGAGCCTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCAAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACGCAGTGTCCAATTGAACCTGGATCAAATTTCACTTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCACGCTCATAGTGATTGGACCAGAGCTGGTGTCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACAGGTTTTCCATTTCCCGAGCCAGATGGAGAAGAAATAGTGGTACTTGGTTCTTGGTATTATATTGACAATCTGAATGAGGTGGTTGCTAAGGACGTCGAAGAAGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTCGGCCTATGCTCAAATGACAGTACATATCGCTGGCAAGTGGAGTATGGCAAGACGTACCTTCTTCGCCTGGTTAGTGCAGTTATGAATGCAGAACTTTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTAAAACCGATTGTGACCGATAATATCATGATTTCCCCCGGACAAACCATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGTGTTAGGCCAGATGATCAAGACTATGACAAGTCAAATGTTACAGCAATTCTGGAGTATAAAGGCAATTACACAACCCCAGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCAATAGAGTTTACAGACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGATGATGTGACAGTTGCTCTTCTTGCAAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACATTAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGAAAATTTGCCTGATAATACTGAGGTTACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATAGTGTTTCAGGGTACTAATACGATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGATATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGACAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTATTTCAGTCCCAATGAAAGGATGGCTTGCTATCAGGTTCAAAGCAAGTAATCCTGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGAGACACACCGGAGAGGTGCATGCGAGAACCTCCTCCATACATGCCTCCCTGCACTGATTCACCTACCATCAGGTTGCGACCTTCCCACAAATTCAGCAGGACGGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

586

Amino Acids

65.92

Weight (kDa)

4.84

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 146 3.7e-41 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 1.5e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 424 - 550 5.8e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 1006
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 1091
AclWI GGATC 5 cut(s) 328, 1450, 1469, 1493, 1608
AcsI RAATTY 4 cut(s) 325, 1285, 1456, 1739
AcyI GRCGYC 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 9 cut(s) 93, 180, 486, 625, 659, 970, 1085, 1343, 1375
AfiI CCNNNNNNNGG 2 cut(s) 1212, 1535
AgsI TTSAA 3 cut(s) 65, 314, 1558
AjnI CCWGG 5 cut(s) 160, 316, 592, 669, 1570
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 7 cut(s) 401, 821, 1029, 1173, 1428, 1485, 1626
AluI AGCT 7 cut(s) 401, 821, 1029, 1173, 1428, 1485, 1626
Alw21I GWGCWC 2 cut(s) 12, 49
Alw26I GTCTC 3 cut(s) 434, 834, 1644
Alw44I GTGCAC 1 cut(s) 45
AlwI GGATC 5 cut(s) 328, 1450, 1469, 1493, 1608
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 5 cut(s) 102, 164, 229, 604, 875
ApaLI GTGCAC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 851
ApoI RAATTY 4 cut(s) 325, 1285, 1456, 1739
AseI ATTAAT 1 cut(s) 1386
AspS9I GGNCC 3 cut(s) 164, 284, 393
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 3 cut(s) 200, 1075, 1701
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 2 cut(s) 284, 393
BaeGI GKGCMC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 961, 993
Bbv12I GWGCWC 2 cut(s) 12, 49
BbvI GCAGC 1 cut(s) 838
BccI CCATC 5 cut(s) 275, 461, 737, 1533, 1724
BciT130I CCWGG 5 cut(s) 162, 318, 594, 671, 1572
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 3 cut(s) 434, 834, 1644
BfaI CTAG 1 cut(s) 855
BfmI CTRYAG 2 cut(s) 1188, 1210
BfuAI ACCTGC 1 cut(s) 1006
BisI GCNGC 1 cut(s) 852
BlpI GCTNAGC 1 cut(s) 1030
BlsI GCNGC 1 cut(s) 853
Bme1390I CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
Bme18I GGWCC 2 cut(s) 284, 393
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 3 cut(s) 164, 284, 393
BmiI GGNNCC 3 cut(s) 223, 269, 415
BmrFI CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
BmsI GCATC 2 cut(s) 265, 1400
BpmI CTGGAG 1 cut(s) 941
Bpu10I CCTNAGC 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 1030
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 8 cut(s) 161, 263, 272, 409, 790, 801, 1323, 1571
BsaWI WCCGGW 1 cut(s) 1657
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 2 cut(s) 1212, 1535
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 3 cut(s) 167, 248, 826
BseBI CCWGG 5 cut(s) 162, 318, 594, 671, 1572
BseDI CCNNGG 8 cut(s) 161, 263, 272, 409, 790, 801, 1323, 1571
BseGI GGATG 3 cut(s) 748, 1402, 1544
BseLI CCNNNNNNNGG 2 cut(s) 1212, 1535
BseMII CTCAG 3 cut(s) 279, 1251, 1293
BseNI ACTGG 3 cut(s) 167, 248, 826
BseRI GAGGAG 2 cut(s) 549, 1671
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 1 cut(s) 838
BseYI CCCAGC 3 cut(s) 22, 946, 1173
BsgI GTGCAG 2 cut(s) 699, 1684
BshFI GGCC 5 cut(s) 104, 166, 231, 606, 877
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 2 cut(s) 12, 49
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 3 cut(s) 792, 1061, 1658
BslFI GGGAC 1 cut(s) 1511
BslI CCNNNNNNNGG 2 cut(s) 1212, 1535
BsmAI GTCTC 3 cut(s) 434, 834, 1644
BsmFI GGGAC 1 cut(s) 1511
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 5 cut(s) 104, 166, 231, 606, 877
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 12, 49
Bsp1407I TGTACA 2 cut(s) 178, 1083
Bsp143I GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
Bsp1720I GCTNAGC 1 cut(s) 1030
Bsp19I CCATGG 2 cut(s) 409, 801
BspACI CCGC 1 cut(s) 1091
BspANI GGCC 5 cut(s) 104, 166, 231, 606, 877
BspCNI CTCAG 3 cut(s) 280, 1250, 1294
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 3 cut(s) 223, 269, 415
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 5 cut(s) 328, 1450, 1469, 1493, 1608
BspQI GCTCTTC 1 cut(s) 1152
BspT107I GGYRCC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 1060
BsrGI TGTACA 2 cut(s) 178, 1083
BsrI ACTGG 3 cut(s) 167, 248, 826
BssAI RCCGGY 1 cut(s) 1060
BssECI CCNNGG 8 cut(s) 161, 263, 272, 409, 790, 801, 1323, 1571
BssMI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
BssNI GRCGYC 1 cut(s) 537
BssT1I CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
Bst2UI CCWGG 5 cut(s) 162, 318, 594, 671, 1572
Bst4CI ACNGT 7 cut(s) 155, 196, 623, 730, 1141, 1312, 1592
Bst6I CTCTTC 2 cut(s) 1152, 1344
BstACI GRCGYC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 2 cut(s) 178, 1083
BstC8I GCNNGC 6 cut(s) 137, 378, 635, 1036, 1545, 1670
BstDEI CTNAG 5 cut(s) 288, 531, 1030, 1237, 1302
BstDSI CCRYGG 2 cut(s) 409, 801
BstENI CCTNNNNNAGG 1 cut(s) 1210
BstF5I GGATG 3 cut(s) 748, 1402, 1544
BstKTI GATC 6 cut(s) 323, 886, 1445, 1477, 1501, 1603
BstMAI GTCTC 3 cut(s) 434, 834, 1644
BstMBI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
BstMWI GCNNNNNNNGC 4 cut(s) 253, 570, 1035, 1151
BstNI CCWGG 5 cut(s) 162, 318, 594, 671, 1572
BstNSI RCATGY 3 cut(s) 139, 1672, 1693
BstSCI CCNGG 6 cut(s) 160, 316, 592, 669, 790, 1570
BstSFI CTRYAG 2 cut(s) 1188, 1210
BstSLI GKGCMC 1 cut(s) 49
BstV1I GCAGC 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 1474
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 1474
BsuRI GGCC 5 cut(s) 104, 166, 231, 606, 877
BtgI CCRYGG 2 cut(s) 409, 801
BtsCI GGATG 3 cut(s) 748, 1402, 1544
BtsI GCAGTG 2 cut(s) 49, 308
BtsIMutI CAGTG 6 cut(s) 49, 241, 308, 726, 1588, 1701
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 6 cut(s) 137, 378, 635, 1036, 1545, 1670
CciI TCATGA 1 cut(s) 780
Cfr10I RCCGGY 1 cut(s) 1060
Cfr13I GGNCC 3 cut(s) 164, 284, 393
Csp6I GTAC 9 cut(s) 92, 179, 485, 624, 658, 969, 1084, 1342, 1374
CviQI GTAC 9 cut(s) 92, 179, 485, 624, 658, 969, 1084, 1342, 1374
DdeI CTNAG 5 cut(s) 288, 531, 1030, 1237, 1302
DpnI GATC 6 cut(s) 322, 885, 1444, 1476, 1500, 1602
DpnII GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
DraI TTTAAA 1 cut(s) 1161
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 1152, 1344
EarI CTCTTC 2 cut(s) 1152, 1344
Eco130I CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
Eco47I GGWCC 2 cut(s) 284, 393
Eco88I CYCGRG 1 cut(s) 458
EcoNI CCTNNNNNAGG 1 cut(s) 1210
EcoRII CCWGG 5 cut(s) 160, 316, 592, 669, 1570
EcoT14I CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
EcoT22I ATGCAT 1 cut(s) 1393
ErhI CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
FalI AAGNNNNNCTT 6 cut(s) 256, 288, 351, 383, 1527, 1559
FaqI GGGAC 1 cut(s) 1511
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 1 cut(s) 852
FokI GGATG 3 cut(s) 755, 1389, 1551
Fsp4HI GCNGC 1 cut(s) 852
FspBI CTAG 1 cut(s) 855
GluI GCNGC 1 cut(s) 852
GsaI CCCAGC 3 cut(s) 26, 950, 1177
GsuI CTGGAG 1 cut(s) 941
HaeIII GGCC 5 cut(s) 104, 166, 231, 606, 877
HapII CCGG 3 cut(s) 792, 1061, 1658
Hin1I GRCGYC 1 cut(s) 537
HinfI GANTC 3 cut(s) 214, 1399, 1706
HpaII CCGG 3 cut(s) 792, 1061, 1658
HphI GGTGA 3 cut(s) 200, 1075, 1701
Hpy166II GTNNAC 4 cut(s) 47, 76, 179, 1011
Hpy188III TCNNGA 7 cut(s) 211, 287, 458, 781, 887, 920, 1024
Hpy8I GTNNAC 4 cut(s) 47, 76, 179, 1011
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 6 cut(s) 59, 353, 539, 671, 987, 1740
HpyCH4III ACNGT 7 cut(s) 155, 196, 623, 730, 1141, 1312, 1592
HpyCH4IV ACGT 2 cut(s) 537, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 570, 1035, 1151
HpyF3I CTNAG 5 cut(s) 288, 531, 1030, 1237, 1302
HpySE526I ACGT 2 cut(s) 537, 656
Hsp92I GRCGYC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 3 cut(s) 15, 221, 746
Lsp1109I GCAGC 1 cut(s) 838
LweI GCATC 2 cut(s) 265, 1400
MaeI CTAG 1 cut(s) 855
MaeII ACGT 2 cut(s) 537, 656
MaeIII GTNAC 6 cut(s) 295, 766, 814, 907, 1135, 1306
MalI GATC 6 cut(s) 322, 885, 1444, 1476, 1500, 1602
MboI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
MboII GAAGA 7 cut(s) 485, 554, 656, 1139, 1361, 1519, 1654
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 1474
MhlI GDGCHC 2 cut(s) 12, 49
MmeI TCCRAC 2 cut(s) 1208, 1420
Mph1103I ATGCAT 1 cut(s) 1393
MseI TTAA 3 cut(s) 1160, 1179, 1386
MslI CAYNNNNRTG 4 cut(s) 81, 246, 370, 1416
MspI CCGG 3 cut(s) 792, 1061, 1658
MspR9I CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
MssI GTTTAAAC 1 cut(s) 1161
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 5 cut(s) 162, 318, 594, 671, 1572
MwoI GCNNNNNNNGC 4 cut(s) 253, 570, 1035, 1151
NciI CCSGG 1 cut(s) 792
NcoI CCATGG 2 cut(s) 409, 801
NdeI CATATG 1 cut(s) 751
NdeII GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
NlaIV GGNNCC 3 cut(s) 223, 269, 415
NmuCI GTSAC 4 cut(s) 295, 766, 814, 1135
NsiI ATGCAT 1 cut(s) 1393
NspI RCATGY 3 cut(s) 139, 1672, 1693
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 2 cut(s) 139, 1672
PagI TCATGA 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 1152
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 3 cut(s) 214, 1399, 1706
PkrI GCNGC 1 cut(s) 853
PmeI GTTTAAAC 1 cut(s) 1161
PshBI ATTAAT 1 cut(s) 1386
Psp6I CCWGG 5 cut(s) 160, 316, 592, 669, 1570
PspFI CCCAGC 3 cut(s) 22, 946, 1173
PspGI CCWGG 5 cut(s) 160, 316, 592, 669, 1570
PspN4I GGNNCC 3 cut(s) 223, 269, 415
PspPI GGNCC 3 cut(s) 164, 284, 393
PsuI RGATCY 1 cut(s) 1474
RsaI GTAC 9 cut(s) 93, 180, 486, 625, 659, 970, 1085, 1343, 1375
RsaNI GTAC 9 cut(s) 92, 179, 485, 624, 658, 969, 1084, 1342, 1374
RseI CAYNNNNRTG 4 cut(s) 81, 246, 370, 1416
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 3 cut(s) 1160, 1179, 1386
SatI GCNGC 1 cut(s) 852
Sau3AI GATC 6 cut(s) 320, 883, 1442, 1474, 1498, 1600
Sau96I GGNCC 3 cut(s) 164, 284, 393
ScrFI CCNGG 6 cut(s) 162, 318, 594, 671, 792, 1572
SduI GDGCHC 2 cut(s) 12, 49
SfaNI GCATC 2 cut(s) 265, 1400
SfcI CTRYAG 2 cut(s) 1188, 1210
SinI GGWCC 2 cut(s) 284, 393
SmiMI CAYNNNNRTG 4 cut(s) 81, 246, 370, 1416
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SphI GCATGC 2 cut(s) 139, 1672
SsiI CCGC 1 cut(s) 1091
SspMI CTAG 1 cut(s) 855
StyD4I CCNGG 6 cut(s) 160, 316, 592, 669, 790, 1570
StyI CCWWGG 5 cut(s) 263, 272, 409, 801, 1323
TaaI ACNGT 7 cut(s) 155, 196, 623, 730, 1141, 1312, 1592
TaiI ACGT 2 cut(s) 540, 659
TaqI TCGA 2 cut(s) 540, 1603
TaqII GACCGA 1 cut(s) 785
TatI WGTACW 5 cut(s) 91, 178, 623, 1083, 1341
TfiI GAWTC 3 cut(s) 214, 1399, 1706
Tru1I TTAA 3 cut(s) 1160, 1179, 1386
Tru9I TTAA 3 cut(s) 1160, 1179, 1386
TscAI CASTG 6 cut(s) 49, 248, 308, 733, 1595, 1708
TseFI GTSAC 4 cut(s) 295, 766, 814, 1135
TseI GCWGC 1 cut(s) 851
Tsp45I GTSAC 4 cut(s) 295, 766, 814, 1135
TspDTI ATGAA 6 cut(s) 596, 701, 1115, 1296, 1362, 1547
TspRI CASTG 6 cut(s) 49, 248, 308, 733, 1595, 1708
VneI GTGCAC 1 cut(s) 45
VpaK11BI GGWCC 2 cut(s) 284, 393
VspI ATTAAT 1 cut(s) 1386
XagI CCTNNNNNAGG 1 cut(s) 1210
XapI RAATTY 4 cut(s) 325, 1285, 1456, 1739
XceI RCATGY 3 cut(s) 139, 1672, 1693
XcmI CCANNNNNNNNNTGG 4 cut(s) 136, 270, 808, 1536
XspI CTAG 1 cut(s) 855
ZraI GACGTC 1 cut(s) 538
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.