Rh2BG468300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001075
Physical Location & Seq
Reverse (-)
66128365 .. 66141952
13588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG468300.1

Sequence Viewer

Length: 1338 bp
ATGTCTATTATACCCTTTAAACTAAAAAGTGAAACTAATAAGGTCCATAAGAATGTCATCCCAAAAGGATCTTGGTATTTAATTGAACGTCTGAACGAGGTGGTTACTGAGGATGTCGAAGAAGGTCTTGACACTCCACGGTCTGATTGCTACACCATTAATGGACAACCTGGAGATTTTGGCTGGTGCTCAAATGAAAGTACACATCGCTGGCAAGTAGAGTATAACAAGACGTATCTTCTTCGTATTGTCAATGCTGTGATGAATGCAGAACTTTACTTTGCCATTGCTGAACATACACTCACTGTTGTTGGTATGGATGGATCATATGTTAAACCCATCGTGACCGATTATATTATGATTTCTGCCGGACAAACCATGGATATTTTGGTCACCGCCAACCAGCGTCTTGGCCGATACTACATGGCTGGAAGACAATTTGACACCGCTAAGTCAGCCGTTGAAGGCTATGACAAATCAAATGTGACAGCAATTCTGGAGTATAAAGGCAATTACTCTCACCCGGTATACCCAATCTTTCCATCCACCCTTCCTGCGTTTGAGGATATAGAATCTGCGGTAAAGTTCACAAGTCGTCTTAAGAGCTTAGCAAGCATAGACCACCCTACAAATGTGCCACAGAATATAACTACCAAAATGTATATAACGGTTGCGATGAATAATGGCACCCTTATTAATGGTGGTGAAGAGATCAAAAGTGGCCTTCTATCAAGTTTAAACAATCTAAGTTGGGTTAATCCATCAACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGATTTTATACTTCAGATTTTCCTGATTTTCCACCAGCATTATTTGACTTCGTATCTGATGATCTGGCTCCTGATAGAACTATTGCAACTGTCAAAGCAACTAGAGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATTGTATTTCAGGGCTTTCTTATTCCAAGCAGCTGTCATGATGGTTATTCTGGGATTCAGCAAAAACATGCAGCCTTGTTCATCTTTGGTGATTCAGTATTTGATGTTGGAAACAATAACTACATAAATACAACCACTACTTTTCAGTCAAATTTCTGGCCCTATGGGGAATCTTATTTCAGATACCCAACTGGCAGATTTTCTGATGGGCGTCTAATCCCTGATTTTATAGCTGAGTATGCAAATTTACCTTTTATACAACCATACTTACAACCCGAGTTCGATGACTATACTTATGGGGTGAACTTTGCTTCTGGTGGGGGTGGTGCTCTAGCTGAAACATATCAAGCATTTGTATGTGATCTTGCTTTAAATTTGCCTTATCAAAACTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

445

Amino Acids

49.99

Weight (kDa)

4.75

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 18 - 171 7.1e-33 Multicopper oxidase
Lipase_GDSL PF00657 339 - 424 2.6e-07 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 686
AccI GTMKAC 1 cut(s) 528
AciI CCGC 3 cut(s) 396, 447, 578
AclWI GGATC 2 cut(s) 76, 331
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 3 cut(s) 1090, 1183, 1312
AcuI CTGAAG 1 cut(s) 798
AcyI GRCGYC 1 cut(s) 1150
AfaI GTAC 2 cut(s) 202, 923
AfiI CCNNNNNNNGG 1 cut(s) 789
AflII CTTAAG 1 cut(s) 599
AgsI TTSAA 2 cut(s) 86, 464
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 4 cut(s) 606, 972, 1172, 1274
AluI AGCT 4 cut(s) 606, 972, 1172, 1274
Alw21I GWGCWC 2 cut(s) 191, 1270
AlwI GGATC 2 cut(s) 76, 331
Ama87I CYCGRG 1 cut(s) 1214
AoxI GGCC 3 cut(s) 412, 721, 1097
ApeKI GCWGC 2 cut(s) 969, 1010
ApoI RAATTY 3 cut(s) 1090, 1183, 1312
AseI ATTAAT 2 cut(s) 159, 696
AspS9I GGNCC 2 cut(s) 43, 1098
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 5 cut(s) 385, 512, 716, 1040, 1252
AvaI CYCGRG 1 cut(s) 1214
AvaII GGWCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 2 cut(s) 191, 1270
BbvI GCAGC 2 cut(s) 981, 1022
BccI CCATC 6 cut(s) 314, 347, 550, 769, 974, 1139
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 171
BcnI CCSGG 1 cut(s) 524
BfaI CTAG 2 cut(s) 903, 1271
BfmI CTRYAG 1 cut(s) 787
BfrI CTTAAG 1 cut(s) 599
BisI GCNGC 2 cut(s) 970, 1011
BlpI GCTNAGC 1 cut(s) 607
BlsI GCNGC 2 cut(s) 971, 1012
Bme1390I CCNGG 2 cut(s) 171, 524
Bme18I GGWCC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 1214
BmgT120I GGNCC 2 cut(s) 43, 1098
BmiI GGNNCC 2 cut(s) 688, 870
BmrFI CCNGG 2 cut(s) 171, 524
BpiI GAAGAC 1 cut(s) 439
BpmI CTGGAG 2 cut(s) 192, 518
Bpu1102I GCTNAGC 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 1150
BsaJI CCNNGG 2 cut(s) 137, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 789
Bse1I ACTGG 1 cut(s) 1135
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 137, 378
BseGI GGATG 4 cut(s) 57, 118, 325, 542
BseLI CCNNNNNNNGG 1 cut(s) 789
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 2 cut(s) 99, 1164
BseNI ACTGG 1 cut(s) 1135
BseXI GCAGC 2 cut(s) 981, 1022
BshFI GGCC 3 cut(s) 414, 723, 1099
BshNI GGYRCC 1 cut(s) 686
BsiHKAI GWGCWC 2 cut(s) 191, 1270
BsiHKCI CYCGRG 1 cut(s) 1214
BsiSI CCGG 2 cut(s) 369, 524
BslI CCNNNNNNNGG 1 cut(s) 789
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 3 cut(s) 414, 723, 1099
BsoBI CYCGRG 1 cut(s) 1214
Bsp1286I GDGCHC 2 cut(s) 191, 1270
Bsp143I GATC 5 cut(s) 68, 323, 711, 862, 1300
Bsp1720I GCTNAGC 1 cut(s) 607
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 3 cut(s) 396, 447, 578
BspANI GGCC 3 cut(s) 414, 723, 1099
BspCNI CTCAG 2 cut(s) 100, 1165
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 2 cut(s) 688, 870
BspPI GGATC 2 cut(s) 76, 331
BspT107I GGYRCC 1 cut(s) 686
BspTI CTTAAG 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 1135
BssECI CCNNGG 2 cut(s) 137, 378
BssMI GATC 5 cut(s) 68, 323, 711, 862, 1300
BssNAI GTATAC 1 cut(s) 529
BssNI GRCGYC 1 cut(s) 1150
BssT1I CCWWGG 1 cut(s) 378
Bst1107I GTATAC 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 4 cut(s) 141, 307, 670, 892
Bst6I CTCTTC 2 cut(s) 702, 924
BstACI GRCGYC 1 cut(s) 1150
BstAFI CTTAAG 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 212, 613
BstDEI CTNAG 5 cut(s) 108, 450, 607, 746, 1173
BstDSI CCRYGG 2 cut(s) 137, 378
BstEII GGTNACC 1 cut(s) 391
BstENI CCTNNNNNAGG 1 cut(s) 787
BstF5I GGATG 4 cut(s) 57, 118, 325, 542
BstKTI GATC 5 cut(s) 71, 326, 714, 865, 1303
BstMBI GATC 5 cut(s) 68, 323, 711, 862, 1300
BstMWI GCNNNNNNNGC 3 cut(s) 455, 612, 1178
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 1010
BstPI GGTNACC 1 cut(s) 391
BstSCI CCNGG 2 cut(s) 169, 522
BstSFI CTRYAG 1 cut(s) 787
BstV1I GCAGC 2 cut(s) 981, 1022
BstV2I GAAGAC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 1 cut(s) 68
BstZ17I GTATAC 1 cut(s) 529
BsuRI GGCC 3 cut(s) 414, 723, 1099
BtgI CCRYGG 2 cut(s) 137, 378
BtgZI GCGATG 2 cut(s) 191, 689
BtsCI GGATG 4 cut(s) 57, 118, 325, 542
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 212, 613
CciI TCATGA 1 cut(s) 976
Cfr13I GGNCC 2 cut(s) 43, 1098
CseI GACGC 2 cut(s) 395, 1139
Csp6I GTAC 2 cut(s) 201, 922
CviAII CATG 5 cut(s) 379, 424, 796, 977, 1007
CviQI GTAC 2 cut(s) 201, 922
DdeI CTNAG 5 cut(s) 108, 450, 607, 746, 1173
DpnI GATC 5 cut(s) 70, 325, 713, 864, 1302
DpnII GATC 5 cut(s) 68, 323, 711, 862, 1300
DraI TTTAAA 3 cut(s) 19, 738, 1311
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 702, 924
EarI CTCTTC 2 cut(s) 702, 924
Eco130I CCWWGG 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 1214
Eco91I GGTNACC 1 cut(s) 391
EcoNI CCTNNNNNAGG 1 cut(s) 787
EcoO65I GGTNACC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 5 cut(s) 382, 427, 799, 980, 1010
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FatI CATG 5 cut(s) 378, 423, 795, 976, 1006
FauNDI CATATG 1 cut(s) 328
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 970, 1011
FokI GGATG 4 cut(s) 44, 125, 332, 529
Fsp4HI GCNGC 2 cut(s) 970, 1011
FspBI CTAG 2 cut(s) 903, 1271
GluI GCNGC 2 cut(s) 970, 1011
GsuI CTGGAG 2 cut(s) 192, 518
HaeIII GGCC 3 cut(s) 414, 723, 1099
HapII CCGG 2 cut(s) 369, 524
HgaI GACGC 2 cut(s) 395, 1139
Hin1I GRCGYC 1 cut(s) 1150
Hin1II CATG 5 cut(s) 382, 427, 799, 980, 1010
HinfI GANTC 5 cut(s) 572, 906, 994, 1031, 1109
HpaII CCGG 2 cut(s) 369, 524
HphI GGTGA 5 cut(s) 385, 512, 716, 1040, 1252
Hpy166II GTNNAC 4 cut(s) 203, 529, 588, 1243
Hpy188I TCNGA 6 cut(s) 93, 145, 817, 859, 1121, 1144
Hpy188III TCNNGA 6 cut(s) 128, 343, 497, 824, 872, 977
Hpy8I GTNNAC 4 cut(s) 203, 529, 588, 1243
HpyAV CCTTC 4 cut(s) 116, 458, 560, 734
HpyCH4III ACNGT 4 cut(s) 141, 307, 670, 892
HpyCH4IV ACGT 2 cut(s) 88, 233
HpyCH4V TGCA 4 cut(s) 269, 887, 1010, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 612, 1178
HpyF3I CTNAG 5 cut(s) 108, 450, 607, 746, 1173
HpySE526I ACGT 2 cut(s) 88, 233
Hsp92I GRCGYC 1 cut(s) 1150
Hsp92II CATG 5 cut(s) 382, 427, 799, 980, 1010
Kzo9I GATC 5 cut(s) 68, 323, 711, 862, 1300
LmnI GCTCC 1 cut(s) 874
Lsp1109I GCAGC 2 cut(s) 981, 1022
MaeI CTAG 2 cut(s) 903, 1271
MaeII ACGT 2 cut(s) 88, 233
MaeIII GTNAC 4 cut(s) 103, 343, 391, 484
MalI GATC 5 cut(s) 70, 325, 713, 864, 1302
MboI GATC 5 cut(s) 68, 323, 711, 862, 1300
MboII GAAGA 6 cut(s) 131, 230, 233, 444, 719, 941
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 2 cut(s) 191, 1270
MluCI AATT 8 cut(s) 81, 437, 492, 511, 939, 1090, 1183, 1312
MlyI GAGTC 1 cut(s) 915
MmeI TCCRAC 1 cut(s) 1027
MnlI CCTC 4 cut(s) 91, 103, 556, 925
MseI TTAA 9 cut(s) 18, 80, 159, 333, 600, 696, 737, 756, 1310
MslI CAYNNNNRTG 2 cut(s) 51, 1294
MspA1I CMGCKG 1 cut(s) 972
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 2 cut(s) 369, 524
MspR9I CCNGG 2 cut(s) 171, 524
MssI GTTTAAAC 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 455, 612, 1178
NciI CCSGG 1 cut(s) 524
NcoI CCATGG 1 cut(s) 378
NdeI CATATG 1 cut(s) 328
NdeII GATC 5 cut(s) 68, 323, 711, 862, 1300
NlaIII CATG 5 cut(s) 382, 427, 799, 980, 1010
NlaIV GGNNCC 2 cut(s) 688, 870
NmuCI GTSAC 3 cut(s) 343, 391, 484
NspI RCATGY 1 cut(s) 1010
PagI TCATGA 1 cut(s) 976
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 4 cut(s) 572, 994, 1031, 1109
PkrI GCNGC 2 cut(s) 971, 1012
PleI GAGTC 1 cut(s) 914
PmeI GTTTAAAC 1 cut(s) 738
PpsI GAGTC 1 cut(s) 914
PshBI ATTAAT 2 cut(s) 159, 696
Psp6I CCWGG 1 cut(s) 169
PspEI GGTNACC 1 cut(s) 391
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 688, 870
PspPI GGNCC 2 cut(s) 43, 1098
PsuI RGATCY 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 972
RsaI GTAC 2 cut(s) 202, 923
RsaNI GTAC 2 cut(s) 201, 922
RseI CAYNNNNRTG 2 cut(s) 51, 1294
SaqAI TTAA 9 cut(s) 18, 80, 159, 333, 600, 696, 737, 756, 1310
SatI GCNGC 2 cut(s) 970, 1011
Sau3AI GATC 5 cut(s) 68, 323, 711, 862, 1300
Sau96I GGNCC 2 cut(s) 43, 1098
SchI GAGTC 1 cut(s) 915
ScrFI CCNGG 2 cut(s) 171, 524
SduI GDGCHC 2 cut(s) 191, 1270
SfcI CTRYAG 1 cut(s) 787
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 2 cut(s) 51, 1294
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 8 cut(s) 81, 437, 492, 511, 939, 1090, 1183, 1312
SsiI CCGC 3 cut(s) 396, 447, 578
SspMI CTAG 2 cut(s) 903, 1271
StyD4I CCNGG 2 cut(s) 169, 522
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 141, 307, 670, 892
TaiI ACGT 2 cut(s) 91, 236
TaqI TCGA 2 cut(s) 117, 1221
TaqII GACCGA 1 cut(s) 362
TasI AATT 8 cut(s) 81, 437, 492, 511, 939, 1090, 1183, 1312
TatI WGTACW 2 cut(s) 200, 921
TfiI GAWTC 4 cut(s) 572, 994, 1031, 1109
Tru1I TTAA 9 cut(s) 18, 80, 159, 333, 600, 696, 737, 756, 1310
Tru9I TTAA 9 cut(s) 18, 80, 159, 333, 600, 696, 737, 756, 1310
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 3 cut(s) 343, 391, 484
TseI GCWGC 2 cut(s) 969, 1010
Tsp45I GTSAC 3 cut(s) 343, 391, 484
TspDTI ATGAA 5 cut(s) 210, 278, 692, 942, 1009
TspRI CASTG 1 cut(s) 310
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 43
VspI ATTAAT 2 cut(s) 159, 696
XagI CCTNNNNNAGG 1 cut(s) 787
XapI RAATTY 3 cut(s) 1090, 1183, 1312
XceI RCATGY 1 cut(s) 1010
XcmI CCANNNNNNNNNTGG 2 cut(s) 69, 385
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 2 cut(s) 903, 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.