RchiOBHm_Chr6g0274121

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001075
Physical Location & Seq
Reverse (-)
35335328 .. 35352214
16887 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24592

Sequence Viewer

Length: 1770 bp
ATGGGTATCTTTCCAGGTTTTCTGGGTTTGTTTCTGCTATGTGGGATGCTGCATTGCATTGTTGAATGCCATGTCCATTACCATGACTTTGTACTCAAAAAGGAGAACTTTACAAGATTATGTTACACAAAGAGCATGCTGGTAGTGAATGGCAGTTTTCCGGGGCCAGTCATTCGTGTGCAAAAGGGGGATACTGTCTTTGTCAATGTTCACAATCAAGGAGATTATGGACTTACTATTCACTGGCATGGAGTACATCAACCAAGAAATCCATGGTCAGATGGTCCTGAATATATTACACAATGTGCCATCAAACCTGGCTCAAATTTTACTTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGAATGGACGAGAGCTACTGTCCATGGTGCCATTGTTATTATGCCTTTAAATAATACGAGTTTTCCGTTTCCTGAACCTGATGGACAAGAAATCATTGTACTCAGTTCTTGGTATAACAGTACTGAAGATGTGAGTGACATAGTTGCTGAAGCCCTTCAAGATGGAGGTGATACTCCCGACTCTGATTGCTACACCATCAATGGACAACCAGGAGATTTTCTCCCATGCTCAAATGATAGTACATACCATTGGCAGGTGGAGTATGGTAAGACCTATCTTCTACGTCTTGTCAATGCAGTGATGAATTCAGAACTGTTCTTTGCAATTGCTGAACATAGCCTCACCGTTGTTGGTATCGACGCATCCTATGTGAAACCCATCGTGACCGATTATATCATGATTAGCGCTGGACAAACAATGGATATTTTGATCACCGCAAACCAATCCCCTGGCGCTTACTATATGGCTACTAGACAATATAACACCGTGAGGAAAAATATTGATGACTACGATAATTCAAATGTTACTGCAATTCTTCAGTACAAAGAGAAGTACACACCCTTGATGGACCCAGTTTTTCCAAGCATCCTCCCTACTTATTTTGATATAGTATCAGGAAGAAAGTTCACAAGGCGTATTAAGAGCTTAGCAAGCATAGAGCACCCGACAAATGTGCCGAAGAATATATCTACTAGAATGTTTATAACAGCTGCTTCAAATTATGGTTTCATTAATCGTTCTGGCGAGTATGAAACTGGTGTTACTACTAGTCTAAACAATTTAAGTTGGGTTAATCCAGAAACAGATGTGCTACTTTCCTACTACAGGAACATAACAGGGTTTTACACGCCAGACTTTCCTGATTATCCACCATTTATATTTGACTTTGTGTCAGAAGATTTTGCTCCTGATACTGTCCCAACAATAAAAGGAACCAAGGTGAAGGTGTTAGAATACGATGAAGAGGTTGAAATAGTGTTTCAGGGTACTGATCTCATGAGAGCATCTGAGGATCATCCAATGCACATGCATGGTTATAGTTTTTATGTGGTTGGATCAGGAACTGGAAATTTTGACAATGAGACAGACCCCAAAGGCTACAATTTATTTGATCCTCCAAAGATGAATACTGTTTCGCTCCCAAAAGTAGGATGGGTAGCTATCAGATTCAAAGCAAGTAACCCCGGAGTTTGGTTCTGGCACTGTCATTTTGATCGACATTTGACTTGGGGTATGAGCTTCGTGATAATAGTTAAAGATGGAGGTACGCCAGAAACGAGCATACGTAAGCCACCTCCAAACATGCCTCAATGTACTAGCTCATCTACTATTAAGCTGCAACCTTTCCATGATTTCAGCAGAACAGGCGACAAAAGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

589

Amino Acids

66.57

Weight (kDa)

5.29

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 144 8.5e-41 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 4.4e-37 Multicopper oxidase
Cu-oxidase_2 PF07731 416 - 549 8.6e-39 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AarI CACCTGC 1 cut(s) 631
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 822
AclWI GGATC 3 cut(s) 1407, 1450, 1493
AcsI RAATTY 3 cut(s) 325, 691, 1456
AcuI CTGAAG 3 cut(s) 531, 555, 908
AdeI CACNNNGTG 1 cut(s) 305
AfeI AGCGCT 1 cut(s) 793
AfiI CCNNNNNNNGG 4 cut(s) 640, 1212, 1535, 1578
AgsI TTSAA 6 cut(s) 65, 545, 906, 1104, 1358, 1558
AhdI GACNNNNNGTC 1 cut(s) 1276
AhlI ACTAGT 1 cut(s) 1154
AjnI CCWGG 4 cut(s) 13, 316, 595, 835
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 7 cut(s) 401, 1032, 1097, 1547, 1626, 1707, 1723
AluI AGCT 7 cut(s) 401, 1032, 1097, 1547, 1626, 1707, 1723
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 1 cut(s) 1463
AlwI GGATC 3 cut(s) 1407, 1450, 1493
AlwNI CAGNNNCTG 1 cut(s) 1451
Aor51HI AGCGCT 1 cut(s) 793
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 3 cut(s) 49, 1097, 1723
ApoI RAATTY 3 cut(s) 325, 691, 1456
AseI ATTAAT 1 cut(s) 1119
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 2 cut(s) 794, 842
AspS9I GGNCC 3 cut(s) 164, 284, 955
AsuC2I CCSGG 2 cut(s) 162, 1572
AsuHPI GGTGA 4 cut(s) 566, 721, 811, 1339
AvaII GGWCC 2 cut(s) 284, 955
BanI GGYRCC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 3 cut(s) 36, 1084, 1710
BccI CCATC 9 cut(s) 275, 317, 461, 542, 590, 773, 946, 1533, 1640
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciT130I CCWGG 4 cut(s) 15, 318, 597, 837
BciVI GTATCC 1 cut(s) 184
BclI TGATCA 1 cut(s) 816
BcnI CCSGG 2 cut(s) 162, 1572
BcoDI GTCTC 1 cut(s) 1463
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 5 cut(s) 858, 1080, 1155, 1704, 1768
BfmI CTRYAG 1 cut(s) 1210
BfoI RGCGCY 2 cut(s) 795, 843
BfuAI ACCTGC 1 cut(s) 631
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 3 cut(s) 50, 1098, 1724
BlpI GCTNAGC 1 cut(s) 1033
BlsI GCNGC 3 cut(s) 51, 1099, 1725
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 6 cut(s) 15, 162, 318, 597, 837, 1572
Bme18I GGWCC 2 cut(s) 284, 955
BmeRI GACNNNNNGTC 1 cut(s) 1276
BmgT120I GGNCC 3 cut(s) 164, 284, 955
BmiI GGNNCC 4 cut(s) 165, 415, 957, 1321
BmrFI CCNGG 6 cut(s) 15, 162, 318, 597, 837, 1572
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 4 cut(s) 36, 758, 981, 1400
BmuI ACTGGG 1 cut(s) 953
BplI GAGNNNNNCTC 2 cut(s) 591, 623
Bpu1102I GCTNAGC 1 cut(s) 1033
BpuMI CCSGG 2 cut(s) 162, 1572
BsaAI YACGTR 1 cut(s) 1673
BsaJI CCNNGG 6 cut(s) 161, 272, 409, 835, 1323, 1570
Bsc4I CCNNNNNNNGG 4 cut(s) 640, 1212, 1535, 1578
Bse1I ACTGG 5 cut(s) 167, 248, 959, 1147, 1456
Bse3DI GCAATG 1 cut(s) 52
BseBI CCWGG 4 cut(s) 15, 318, 597, 837
BseDI CCNNGG 6 cut(s) 161, 272, 409, 835, 1323, 1570
BseGI GGATG 5 cut(s) 51, 749, 972, 1402, 1544
BseLI CCNNNNNNNGG 4 cut(s) 640, 1212, 1535, 1578
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 2 cut(s) 502, 1386
BseNI ACTGG 5 cut(s) 167, 248, 959, 1147, 1456
BseXI GCAGC 3 cut(s) 36, 1084, 1710
BshFI GGCC 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 1050
BsiSI CCGG 2 cut(s) 161, 1572
BslFI GGGAC 1 cut(s) 1289
BslI CCNNNNNNNGG 4 cut(s) 640, 1212, 1535, 1578
BsmAI GTCTC 1 cut(s) 1463
BsmFI GGGAC 1 cut(s) 1289
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 1050
Bsp143I GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
Bsp1720I GCTNAGC 1 cut(s) 1033
Bsp19I CCATGG 2 cut(s) 272, 409
BspACI CCGC 1 cut(s) 822
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 2 cut(s) 501, 1387
BspHI TCATGA 2 cut(s) 783, 1383
BspLI GGNNCC 4 cut(s) 165, 415, 957, 1321
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 3 cut(s) 1407, 1450, 1493
BspT107I GGYRCC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 52
BsrI ACTGG 5 cut(s) 167, 248, 959, 1147, 1456
BssECI CCNNGG 6 cut(s) 161, 272, 409, 835, 1323, 1570
BssMI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
BssT1I CCWWGG 3 cut(s) 272, 409, 1323
Bst2UI CCWGG 4 cut(s) 15, 318, 597, 837
Bst4CI ACNGT 9 cut(s) 196, 406, 506, 702, 733, 874, 1303, 1519, 1592
Bst6I CTCTTC 1 cut(s) 1344
BstBAI YACGTR 1 cut(s) 1673
BstC8I GCNNGC 3 cut(s) 137, 378, 1039
BstDEI CTNAG 3 cut(s) 488, 1033, 1395
BstDSI CCRYGG 2 cut(s) 272, 409
BstENI CCTNNNNNAGG 1 cut(s) 1210
BstF5I GGATG 5 cut(s) 51, 749, 972, 1402, 1544
BstH2I RGCGCY 2 cut(s) 795, 843
BstHHI GCGC 2 cut(s) 794, 842
BstKTI GATC 6 cut(s) 819, 1381, 1402, 1445, 1501, 1603
BstMAI GTCTC 1 cut(s) 1463
BstMBI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
BstMWI GCNNNNNNNGC 2 cut(s) 1038, 1752
BstNI CCWGG 4 cut(s) 15, 318, 597, 837
BstNSI RCATGY 4 cut(s) 139, 380, 1417, 1693
BstSCI CCNGG 6 cut(s) 13, 160, 316, 595, 835, 1570
BstSFI CTRYAG 1 cut(s) 1210
BstSNI TACGTA 1 cut(s) 1673
BstV1I GCAGC 3 cut(s) 36, 1084, 1710
BstXI CCANNNNNNTGG 1 cut(s) 836
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 166
BtgI CCRYGG 2 cut(s) 272, 409
BtsCI GGATG 5 cut(s) 51, 749, 972, 1402, 1544
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 3 cut(s) 241, 690, 1588
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 137, 378, 1039
CaiI CAGNNNCTG 1 cut(s) 1451
CciI TCATGA 2 cut(s) 783, 1383
CfoI GCGC 2 cut(s) 794, 842
Cfr13I GGNCC 3 cut(s) 164, 284, 955
CseI GACGC 1 cut(s) 755
DdeI CTNAG 3 cut(s) 488, 1033, 1395
DpnI GATC 6 cut(s) 818, 1380, 1401, 1444, 1500, 1602
DpnII GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
DraI TTTAAA 1 cut(s) 435
DraIII CACNNNGTG 1 cut(s) 305
DriI GACNNNNNGTC 1 cut(s) 1276
Eam1104I CTCTTC 1 cut(s) 1344
Eam1105I GACNNNNNGTC 1 cut(s) 1276
EarI CTCTTC 1 cut(s) 1344
Eco105I TACGTA 1 cut(s) 1673
Eco130I CCWWGG 3 cut(s) 272, 409, 1323
Eco47I GGWCC 2 cut(s) 284, 955
Eco47III AGCGCT 1 cut(s) 793
Eco57I CTGAAG 3 cut(s) 531, 555, 908
EcoNI CCTNNNNNAGG 1 cut(s) 1210
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 4 cut(s) 13, 316, 595, 835
EcoT14I CCWWGG 3 cut(s) 272, 409, 1323
EcoT22I ATGCAT 1 cut(s) 1419
ErhI CCWWGG 3 cut(s) 272, 409, 1323
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 351, 383
FaqI GGGAC 1 cut(s) 1289
FbaI TGATCA 1 cut(s) 816
Fnu4HI GCNGC 3 cut(s) 50, 1098, 1724
FokI GGATG 5 cut(s) 58, 736, 959, 1389, 1551
Fsp4HI GCNGC 3 cut(s) 50, 1098, 1724
FspBI CTAG 5 cut(s) 858, 1080, 1155, 1704, 1768
GlaI GCGC 2 cut(s) 793, 841
GluI GCNGC 3 cut(s) 50, 1098, 1724
HaeII RGCGCY 2 cut(s) 795, 843
HaeIII GGCC 1 cut(s) 166
HapII CCGG 2 cut(s) 161, 1572
HgaI GACGC 1 cut(s) 755
HhaI GCGC 2 cut(s) 794, 842
Hin6I GCGC 2 cut(s) 792, 840
HinP1I GCGC 2 cut(s) 792, 840
HinfI GANTC 2 cut(s) 566, 1554
HpaII CCGG 2 cut(s) 161, 1572
HphI GGTGA 4 cut(s) 566, 721, 811, 1339
Hpy166II GTNNAC 3 cut(s) 211, 942, 1014
Hpy188I TCNGA 7 cut(s) 280, 352, 571, 697, 1282, 1396, 1553
Hpy8I GTNNAC 3 cut(s) 211, 942, 1014
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 3 cut(s) 353, 551, 1324
HpyCH4III ACNGT 9 cut(s) 196, 406, 506, 702, 733, 874, 1303, 1519, 1592
HpyCH4IV ACGT 2 cut(s) 670, 1672
HpyCH4V TGCA 9 cut(s) 52, 57, 181, 683, 710, 917, 1411, 1417, 1726
HpyF10VI GCNNNNNNNGC 2 cut(s) 1038, 1752
HpyF3I CTNAG 3 cut(s) 488, 1033, 1395
HpySE526I ACGT 2 cut(s) 670, 1672
HspAI GCGC 2 cut(s) 792, 840
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
LmnI GCTCC 2 cut(s) 1297, 1530
Lsp1109I GCAGC 3 cut(s) 36, 1084, 1710
LweI GCATC 4 cut(s) 36, 758, 981, 1400
MaeI CTAG 5 cut(s) 858, 1080, 1155, 1704, 1768
MaeII ACGT 2 cut(s) 670, 1672
MaeIII GTNAC 7 cut(s) 122, 521, 769, 910, 1147, 1565, 1763
MalI GATC 6 cut(s) 818, 1380, 1401, 1444, 1500, 1602
MboI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
MboII GAAGA 7 cut(s) 524, 656, 914, 1017, 1078, 1295, 1361
MfeI CAATTG 1 cut(s) 711
MhlI GDGCHC 1 cut(s) 1050
MluCI AATT 9 cut(s) 325, 691, 711, 901, 918, 1105, 1165, 1456, 1489
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 1 cut(s) 1420
Mph1103I ATGCAT 1 cut(s) 1419
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
MslI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 2 cut(s) 161, 1572
MspR9I CCNGG 6 cut(s) 15, 162, 318, 597, 837, 1572
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 4 cut(s) 15, 318, 597, 837
MwoI GCNNNNNNNGC 2 cut(s) 1038, 1752
NciI CCSGG 2 cut(s) 162, 1572
NcoI CCATGG 2 cut(s) 272, 409
NdeII GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
NlaIV GGNNCC 4 cut(s) 165, 415, 957, 1321
NmuCI GTSAC 2 cut(s) 521, 769
NsiI ATGCAT 1 cut(s) 1419
NspI RCATGY 4 cut(s) 139, 380, 1417, 1693
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 2 cut(s) 139, 380
PagI TCATGA 2 cut(s) 783, 1383
PaqCI CACCTGC 1 cut(s) 631
PcsI WCGNNNNNNNCGW 2 cut(s) 449, 1661
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 1 cut(s) 1554
PkrI GCNGC 3 cut(s) 51, 1099, 1725
PleI GAGTC 1 cut(s) 560
PpsI GAGTC 1 cut(s) 560
Ppu21I YACGTR 1 cut(s) 1673
PshBI ATTAAT 1 cut(s) 1119
PsiI TTATAA 1 cut(s) 1091
Psp6I CCWGG 4 cut(s) 13, 316, 595, 835
PspGI CCWGG 4 cut(s) 13, 316, 595, 835
PspN4I GGNNCC 4 cut(s) 165, 415, 957, 1321
PspPI GGNCC 3 cut(s) 164, 284, 955
PstNI CAGNNNCTG 1 cut(s) 1451
PvuII CAGCTG 1 cut(s) 1097
RseI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
SaqAI TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
SatI GCNGC 3 cut(s) 50, 1098, 1724
Sau3AI GATC 6 cut(s) 816, 1378, 1399, 1442, 1498, 1600
Sau96I GGNCC 3 cut(s) 164, 284, 955
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 6 cut(s) 15, 162, 318, 597, 837, 1572
SduI GDGCHC 1 cut(s) 1050
SfaNI GCATC 4 cut(s) 36, 758, 981, 1400
SfcI CTRYAG 1 cut(s) 1210
SinI GGWCC 2 cut(s) 284, 955
SmiMI CAYNNNNRTG 5 cut(s) 81, 176, 246, 370, 1416
SnaBI TACGTA 1 cut(s) 1673
SpeI ACTAGT 1 cut(s) 1154
SphI GCATGC 2 cut(s) 139, 380
Sse9I AATT 9 cut(s) 325, 691, 711, 901, 918, 1105, 1165, 1456, 1489
SsiI CCGC 1 cut(s) 822
SspI AATATT 1 cut(s) 886
SspMI CTAG 5 cut(s) 858, 1080, 1155, 1704, 1768
StyD4I CCNGG 6 cut(s) 13, 160, 316, 595, 835, 1570
StyI CCWWGG 3 cut(s) 272, 409, 1323
TaaI ACNGT 9 cut(s) 196, 406, 506, 702, 733, 874, 1303, 1519, 1592
TaiI ACGT 2 cut(s) 673, 1675
TaqI TCGA 2 cut(s) 744, 1603
TaqII GACCGA 1 cut(s) 788
TasI AATT 9 cut(s) 325, 691, 711, 901, 918, 1105, 1165, 1456, 1489
TatI WGTACW 8 cut(s) 91, 253, 484, 506, 626, 927, 939, 1700
TfiI GAWTC 1 cut(s) 1554
Tru1I TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
Tru9I TTAA 7 cut(s) 434, 1026, 1119, 1169, 1179, 1641, 1719
TscAI CASTG 3 cut(s) 248, 690, 1595
TseFI GTSAC 2 cut(s) 521, 769
TseI GCWGC 3 cut(s) 49, 1097, 1723
Tsp45I GTSAC 2 cut(s) 521, 769
TspDTI ATGAA 5 cut(s) 704, 1105, 1152, 1362, 1526
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 3 cut(s) 248, 690, 1595
VpaK11BI GGWCC 2 cut(s) 284, 955
VspI ATTAAT 1 cut(s) 1119
XagI CCTNNNNNAGG 1 cut(s) 1210
XapI RAATTY 3 cut(s) 325, 691, 1456
XceI RCATGY 4 cut(s) 139, 380, 1417, 1693
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 1536
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 5 cut(s) 858, 1080, 1155, 1704, 1768
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.