Rh6BG196300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001075
Physical Location & Seq
Reverse (-)
36857002 .. 36863826
6825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG196300.1

Sequence Viewer

Length: 1812 bp
ATGCATATATATAGAACTAGAACAACCAGAACAACTAGTTTCCGCGTGAAGATGGGTGTGCTCCAATGGTTGCTGGGTTCTCTGCTTCTATGCAGTGCGCTTCTTTGCATAGTTGAAGGAAATGTTCACTACTATGACTTTGTACTCAAGGAGGCCAACTTTACAAGGTTATGTCAGACTAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGTCATTCGTGTACAAAAAGGTGATACTGTTTATGTCAATGTCCAAAATCAAGGAGCCTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCAAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACGCAGTGTCCAATTGAACCTGGATCAAATTTCACTTACGAGGTTATATTTTCTGACGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGATTGGACCAGAGCTGGTGTCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACAGGTTTTCCATTTCCCGAGCCAGATGGAGAAGAAATAGTTGTACTTGGTTCTTGGTATTATATTGATAATCTGAATGAGGTGGTTGCTAAGGACGTCGAAGAAGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTCAGCCTATGCTCAAATGACAGTACATATCGCTGGCAAGTGGAGTATGGCAAGACGTACCTTCTTCGCCTGGTTAGTGCAGTTATGAATGCAGAACTCTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTAAAACCGATTGTGACCGATAATATCATGATTTCCCCTGGACAAACCATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGTGTTAGGCCAGATGATCAAGACTATGACAAGTCAAATGTTACAGCAATTCTGGAGTATAAAGGCAATTACACAACCCCAGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCAATAGAGTTTACAGACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGATGATGTGACAGTTGCTCTTCTTGCAAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACGTGAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGATAATTTGCCTGATAATACTGAGGTGACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATAGTGTTTCAGGGTACTAATACGATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGATATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGACAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTATTTCAGTCCCAATGAAAGGATGGGTTGCTATCAGGTTCAAAGCAAGTAATCCTGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGAGACACAACGGAGAGGTGCATGCGAGAACCTCCTCCATACATGCCTCCCTGCACTGATTCACCTACCATCAGGTTGCGACCTTCCCACAAATTCAGCAGGACGGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

603

Amino Acids

68.05

Weight (kDa)

5.08

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 50 - 163 3.9e-41 Multicopper oxidase
Cu-oxidase PF00394 177 - 329 1.8e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 442 - 567 3.6e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 591
Acc36I ACCTGC 1 cut(s) 1057
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 1 cut(s) 45
AciI CCGC 2 cut(s) 43, 1142
AclWI GGATC 5 cut(s) 379, 1501, 1520, 1544, 1659
AcsI RAATTY 3 cut(s) 376, 1507, 1790
AcyI GRCGYC 1 cut(s) 588
AdeI CACNNNGTG 1 cut(s) 354
AfaI GTAC 9 cut(s) 144, 231, 537, 676, 710, 1021, 1136, 1394, 1426
AfiI CCNNNNNNNGG 2 cut(s) 1263, 1586
AgsI TTSAA 3 cut(s) 116, 365, 1609
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 6 cut(s) 211, 367, 643, 720, 841, 1621
AleI CACNNNNGTG 1 cut(s) 421
AluBI AGCT 7 cut(s) 452, 872, 1080, 1224, 1479, 1536, 1677
AluI AGCT 7 cut(s) 452, 872, 1080, 1224, 1479, 1536, 1677
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 3 cut(s) 485, 885, 1695
AlwI GGATC 5 cut(s) 379, 1501, 1520, 1544, 1659
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 4 cut(s) 153, 215, 280, 926
ApeKI GCWGC 1 cut(s) 902
ApoI RAATTY 3 cut(s) 376, 1507, 1790
AseI ATTAAT 1 cut(s) 1437
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 3 cut(s) 215, 335, 444
AsuHPI GGTGA 4 cut(s) 251, 1126, 1369, 1752
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 2 cut(s) 335, 444
BanI GGYRCC 1 cut(s) 464
BarI GAAGNNNNNNTAC 2 cut(s) 1012, 1044
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 889
BccI CCATC 6 cut(s) 46, 326, 512, 788, 1584, 1775
BciT130I CCWGG 6 cut(s) 213, 369, 645, 722, 843, 1623
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 3 cut(s) 485, 885, 1695
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 3 cut(s) 18, 36, 906
BfmI CTRYAG 2 cut(s) 1239, 1261
BfuAI ACCTGC 1 cut(s) 1057
BisI GCNGC 1 cut(s) 903
BlpI GCTNAGC 1 cut(s) 1081
BlsI GCNGC 1 cut(s) 904
Bme1390I CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1623
Bme18I GGWCC 2 cut(s) 335, 444
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 215, 335, 444
BmiI GGNNCC 3 cut(s) 274, 320, 466
BmrFI CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1623
BmsI GCATC 2 cut(s) 316, 1451
BpmI CTGGAG 1 cut(s) 992
Bpu10I CCTNAGC 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 1081
BpuEI CTTGAG 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 588
BsaJI CCNNGG 8 cut(s) 212, 314, 323, 460, 841, 852, 1374, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 789, 819
Bsc4I CCNNNNNNNGG 2 cut(s) 1263, 1586
Bse118I RCCGGY 1 cut(s) 1111
Bse1I ACTGG 3 cut(s) 218, 299, 877
BseBI CCWGG 6 cut(s) 213, 369, 645, 722, 843, 1623
BseDI CCNNGG 8 cut(s) 212, 314, 323, 460, 841, 852, 1374, 1622
BseGI GGATG 3 cut(s) 799, 1453, 1595
BseLI CCNNNNNNNGG 2 cut(s) 1263, 1586
BseMII CTCAG 3 cut(s) 330, 1302, 1344
BseNI ACTGG 3 cut(s) 218, 299, 877
BseRI GAGGAG 2 cut(s) 600, 1722
BseXI GCAGC 1 cut(s) 889
BseYI CCCAGC 3 cut(s) 73, 997, 1224
BsgI GTGCAG 2 cut(s) 750, 1735
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 4 cut(s) 155, 217, 282, 928
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 1 cut(s) 1112
BslFI GGGAC 1 cut(s) 1562
BslI CCNNNNNNNGG 2 cut(s) 1263, 1586
BsmAI GTCTC 3 cut(s) 485, 885, 1695
BsmFI GGGAC 1 cut(s) 1562
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 4 cut(s) 155, 217, 282, 928
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 63
Bsp1407I TGTACA 2 cut(s) 229, 1134
Bsp143I GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
Bsp1720I GCTNAGC 1 cut(s) 1081
Bsp19I CCATGG 2 cut(s) 460, 852
BspACI CCGC 2 cut(s) 43, 1142
BspANI GGCC 4 cut(s) 155, 217, 282, 928
BspCNI CTCAG 3 cut(s) 331, 1301, 1345
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 3 cut(s) 274, 320, 466
BspMI ACCTGC 1 cut(s) 1057
BspPI GGATC 5 cut(s) 379, 1501, 1520, 1544, 1659
BspQI GCTCTTC 1 cut(s) 1203
BspT107I GGYRCC 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 1111
BsrGI TGTACA 2 cut(s) 229, 1134
BsrI ACTGG 3 cut(s) 218, 299, 877
BssAI RCCGGY 1 cut(s) 1111
BssECI CCNNGG 8 cut(s) 212, 314, 323, 460, 841, 852, 1374, 1622
BssMI GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
BssNI GRCGYC 1 cut(s) 588
BssT1I CCWWGG 5 cut(s) 314, 323, 460, 852, 1374
Bst2UI CCWGG 6 cut(s) 213, 369, 645, 722, 843, 1623
Bst4CI ACNGT 7 cut(s) 206, 247, 674, 781, 1192, 1363, 1643
Bst6I CTCTTC 2 cut(s) 1203, 1395
BstACI GRCGYC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 304
BstAUI TGTACA 2 cut(s) 229, 1134
BstC8I GCNNGC 5 cut(s) 188, 429, 686, 1087, 1721
BstDEI CTNAG 6 cut(s) 180, 339, 582, 1081, 1288, 1353
BstDSI CCRYGG 2 cut(s) 460, 852
BstENI CCTNNNNNAGG 1 cut(s) 1261
BstF5I GGATG 3 cut(s) 799, 1453, 1595
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 6 cut(s) 374, 937, 1496, 1528, 1552, 1654
BstMAI GTCTC 3 cut(s) 485, 885, 1695
BstMBI GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
BstMWI GCNNNNNNNGC 4 cut(s) 304, 621, 1086, 1202
BstNI CCWGG 6 cut(s) 213, 369, 645, 722, 843, 1623
BstNSI RCATGY 4 cut(s) 190, 431, 1723, 1744
BstSCI CCNGG 6 cut(s) 211, 367, 643, 720, 841, 1621
BstSFI CTRYAG 2 cut(s) 1239, 1261
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 889
BstX2I RGATCY 1 cut(s) 1525
BstXI CCANNNNNNTGG 1 cut(s) 884
BstYI RGATCY 1 cut(s) 1525
BsuRI GGCC 4 cut(s) 155, 217, 282, 928
BtgI CCRYGG 2 cut(s) 460, 852
BtsCI GGATG 3 cut(s) 799, 1453, 1595
BtsI GCAGTG 2 cut(s) 100, 359
BtsIMutI CAGTG 6 cut(s) 100, 292, 359, 777, 1639, 1752
BveI ACCTGC 1 cut(s) 1057
Cac8I GCNNGC 5 cut(s) 188, 429, 686, 1087, 1721
CciI TCATGA 1 cut(s) 831
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 1111
Cfr13I GGNCC 3 cut(s) 215, 335, 444
Csp6I GTAC 9 cut(s) 143, 230, 536, 675, 709, 1020, 1135, 1393, 1425
CviQI GTAC 9 cut(s) 143, 230, 536, 675, 709, 1020, 1135, 1393, 1425
DdeI CTNAG 6 cut(s) 180, 339, 582, 1081, 1288, 1353
DpnI GATC 6 cut(s) 373, 936, 1495, 1527, 1551, 1653
DpnII GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
DraI TTTAAA 1 cut(s) 1212
DraIII CACNNNGTG 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 1203, 1395
EarI CTCTTC 2 cut(s) 1203, 1395
Eco130I CCWWGG 5 cut(s) 314, 323, 460, 852, 1374
Eco47I GGWCC 2 cut(s) 335, 444
Eco88I CYCGRG 1 cut(s) 509
EcoNI CCTNNNNNAGG 1 cut(s) 1261
EcoRII CCWGG 6 cut(s) 211, 367, 643, 720, 841, 1621
EcoT14I CCWWGG 5 cut(s) 314, 323, 460, 852, 1374
EcoT22I ATGCAT 2 cut(s) 6, 1444
ErhI CCWWGG 5 cut(s) 314, 323, 460, 852, 1374
FalI AAGNNNNNCTT 4 cut(s) 307, 339, 402, 434
FaqI GGGAC 1 cut(s) 1562
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 1 cut(s) 903
FokI GGATG 3 cut(s) 806, 1440, 1602
Fsp4HI GCNGC 1 cut(s) 903
FspBI CTAG 3 cut(s) 18, 36, 906
GlaI GCGC 1 cut(s) 99
GluI GCNGC 1 cut(s) 903
GsaI CCCAGC 3 cut(s) 77, 1001, 1228
GsuI CTGGAG 1 cut(s) 992
HaeIII GGCC 4 cut(s) 155, 217, 282, 928
HapII CCGG 1 cut(s) 1112
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 588
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 2 cut(s) 1450, 1757
HpaII CCGG 1 cut(s) 1112
HphI GGTGA 4 cut(s) 251, 1126, 1369, 1752
Hpy166II GTNNAC 3 cut(s) 127, 230, 1062
Hpy188III TCNNGA 6 cut(s) 338, 509, 832, 938, 971, 1075
Hpy8I GTNNAC 3 cut(s) 127, 230, 1062
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 6 cut(s) 110, 404, 590, 722, 1038, 1791
HpyCH4III ACNGT 7 cut(s) 206, 247, 674, 781, 1192, 1363, 1643
HpyCH4IV ACGT 3 cut(s) 588, 707, 1269
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 621, 1086, 1202
HpyF3I CTNAG 6 cut(s) 180, 339, 582, 1081, 1288, 1353
HpySE526I ACGT 3 cut(s) 588, 707, 1269
Hsp92I GRCGYC 1 cut(s) 588
HspAI GCGC 1 cut(s) 98
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 3 cut(s) 66, 272, 797
Lsp1109I GCAGC 1 cut(s) 889
LweI GCATC 2 cut(s) 316, 1451
MaeI CTAG 3 cut(s) 18, 36, 906
MaeII ACGT 3 cut(s) 588, 707, 1269
MaeIII GTNAC 6 cut(s) 346, 817, 865, 958, 1186, 1357
MalI GATC 6 cut(s) 373, 936, 1495, 1527, 1551, 1653
MboI GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
MboII GAAGA 8 cut(s) 61, 536, 605, 707, 1190, 1412, 1570, 1705
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 1 cut(s) 1525
MhlI GDGCHC 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 1259, 1471
Mph1103I ATGCAT 2 cut(s) 6, 1444
MseI TTAA 3 cut(s) 1211, 1230, 1437
MslI CAYNNNNRTG 4 cut(s) 132, 297, 421, 1467
MspI CCGG 1 cut(s) 1112
MspR9I CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1623
MssI GTTTAAAC 1 cut(s) 1212
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 6 cut(s) 213, 369, 645, 722, 843, 1623
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 304, 621, 1086, 1202
NcoI CCATGG 2 cut(s) 460, 852
NdeI CATATG 1 cut(s) 802
NdeII GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
NlaIV GGNNCC 3 cut(s) 274, 320, 466
NmuCI GTSAC 5 cut(s) 346, 817, 865, 1186, 1357
NsiI ATGCAT 2 cut(s) 6, 1444
NspI RCATGY 4 cut(s) 190, 431, 1723, 1744
OliI CACNNNNGTG 1 cut(s) 421
PaeI GCATGC 3 cut(s) 190, 431, 1723
PagI TCATGA 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 1203
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 2 cut(s) 1450, 1757
PkrI GCNGC 1 cut(s) 904
PmeI GTTTAAAC 1 cut(s) 1212
PshBI ATTAAT 1 cut(s) 1437
Psp6I CCWGG 6 cut(s) 211, 367, 643, 720, 841, 1621
PspFI CCCAGC 3 cut(s) 73, 997, 1224
PspGI CCWGG 6 cut(s) 211, 367, 643, 720, 841, 1621
PspN4I GGNNCC 3 cut(s) 274, 320, 466
PspPI GGNCC 3 cut(s) 215, 335, 444
PsuI RGATCY 1 cut(s) 1525
RsaI GTAC 9 cut(s) 144, 231, 537, 676, 710, 1021, 1136, 1394, 1426
RsaNI GTAC 9 cut(s) 143, 230, 536, 675, 709, 1020, 1135, 1393, 1425
RseI CAYNNNNRTG 4 cut(s) 132, 297, 421, 1467
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 3 cut(s) 1211, 1230, 1437
SatI GCNGC 1 cut(s) 903
Sau3AI GATC 6 cut(s) 371, 934, 1493, 1525, 1549, 1651
Sau96I GGNCC 3 cut(s) 215, 335, 444
ScrFI CCNGG 6 cut(s) 213, 369, 645, 722, 843, 1623
SduI GDGCHC 1 cut(s) 63
SfaNI GCATC 2 cut(s) 316, 1451
SfcI CTRYAG 2 cut(s) 1239, 1261
SinI GGWCC 2 cut(s) 335, 444
SmiMI CAYNNNNRTG 4 cut(s) 132, 297, 421, 1467
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 35
SphI GCATGC 3 cut(s) 190, 431, 1723
SsiI CCGC 2 cut(s) 43, 1142
SspMI CTAG 3 cut(s) 18, 36, 906
StyD4I CCNGG 6 cut(s) 211, 367, 643, 720, 841, 1621
StyI CCWWGG 5 cut(s) 314, 323, 460, 852, 1374
TaaI ACNGT 7 cut(s) 206, 247, 674, 781, 1192, 1363, 1643
TaiI ACGT 3 cut(s) 591, 710, 1272
TaqI TCGA 2 cut(s) 591, 1654
TaqII GACCGA 1 cut(s) 836
TatI WGTACW 6 cut(s) 142, 229, 535, 674, 1134, 1392
TfiI GAWTC 2 cut(s) 1450, 1757
Tru1I TTAA 3 cut(s) 1211, 1230, 1437
Tru9I TTAA 3 cut(s) 1211, 1230, 1437
TscAI CASTG 6 cut(s) 100, 299, 359, 784, 1646, 1759
TseFI GTSAC 5 cut(s) 346, 817, 865, 1186, 1357
TseI GCWGC 1 cut(s) 902
Tsp45I GTSAC 5 cut(s) 346, 817, 865, 1186, 1357
TspDTI ATGAA 5 cut(s) 647, 752, 1166, 1413, 1598
TspGWI ACGGA 1 cut(s) 1724
TspRI CASTG 6 cut(s) 100, 299, 359, 784, 1646, 1759
VpaK11BI GGWCC 2 cut(s) 335, 444
VspI ATTAAT 1 cut(s) 1437
XagI CCTNNNNNAGG 1 cut(s) 1261
XapI RAATTY 3 cut(s) 376, 1507, 1790
XceI RCATGY 4 cut(s) 190, 431, 1723, 1744
XcmI CCANNNNNNNNNTGG 3 cut(s) 321, 859, 1587
XspI CTAG 3 cut(s) 18, 36, 906
ZraI GACGTC 1 cut(s) 589
Zsp2I ATGCAT 2 cut(s) 6, 1444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.