RLG00000020248

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001075
Physical Location & Seq
Reverse (-)
62824360 .. 62829602
5243 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020248

Sequence Viewer

Length: 1776 bp
ATGCATGCTCTGAAGATGGGTGTGCTTGGTCCTTGGCTTCTGTGTGGGTTGCTCTTGCTTCTTTCCATAGTTGAAGGGCGTGTTCATTACTATGACTTTGTACTCAAGGAGGCCAACTTTACAAGGTTATGTAATACTAAGAGCATGCTAGTAGTCAACGATAGTTTTCCTGGACCGACCATTCGCTTGCAGAAAGGAGACACCGTCTATGTCAATGTCCAAAACCAAGGAGACTATGGCCTTACTATTCACTGGCATGGAGTACATCAACCAAGAAATCCTTGGTCAGATGGTCCAGAATATGTCACACAATGTCCTATTGAGCCTAAAACAAATTTCACTTATGAGGTTATATTTTCTGATGAGGAAGGAACACTATGGTGGCATGCCCATAGTGATTGGACTAGAGCTAGTGTCCATGGTGCCATTGTTGTTATGCCCTTAGAAGAGGCAGGTTTTCCATTTGCTGAGCCCGATGGAGAAGAAATAATAATATTTGGATCTTGGTATTTAATTGAACGTCTGAACGAGGTGGTTACTGAGGATGTCGAAGAAGGTCTTGACACTCCTCGGTCTGATTGCTACACCATTAATGGACAACCTGGAGACTTTGGCTGGTGCTCAAATGAAAGTACACATCGCTGGCAAGTAGAGTATAACAAGACGTATCTTCTTCGTATTGTCAATGCTGTGATGAATGCAGAACTCTACTTTGCCATTGCTGAACATACACTCACTGTTGTTGGTATGGATGGATCATATGTCAAACCCATCGTGACAGACTATATTATGATTTCTGCCGGACAAACCATGGATATTTTGGTCACCGCCAACCAGCCTCTTGGCCGATACTACATGGCTGGAAGACAATTTGACACCGCTAAGTCAGCCGTTGAAGGCTATGACAAATCAAATGTGACAGCAATTCTGGAGTATAAAGGCAATTACTCTCACCCGGTATACCCAATCTTTCCATCCACCCTTCCTGCGTTTGAGGATATAAAATCTGCGGTAAAGTTCACAAGTCGTCTTAAGAGCTTAGCAAGCATAGACCACCCTACAAATGTGCCACAGAATATAACTACCAAAATGTATATAACGGTTGCGATGAATAATGGCACCCTTATTAATGGTGGTGAAGAGATCAAAAGTGGCCTTCTATCAAGTTTAAACAATCTAAGTTGGGTTAATCCATCAACAGATGTGCTACTTGCCTACTACAGGAACATGAGTGGATTTTATACTTCAGATTTTCCTGATTTTCCACCAGCAATATTTGACTTCGTATCTGATGATCTGGCTCCTGATAGAACTATTGTAACTGTCAAAGCAACTAGAGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATTGTGTTTCAGGGTACTAATTTGCTTGATGCATCTGAGGATCATCCAATGCACTTGCATGGTTATAGCTTTTATGTAGTTGGATCAGGTTTTGGAGATTTTGACAACGAGACAGACCCTGAAGGGTATAATTTGATTGATCCACCGAAGTTAACCACTGTTTCAGTCCCAAAGAAAGGGTGGGTTGCTATCAGATTCAAAGCCAGTAATCCAGGGGTCTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGTATGAACACTGTGATGATAGTGAAAAATGGTGGCACGCCAGAGACGAGCATACGCAGACCACCTCCATACTTGCCTCCATGTAATGGTTCACCTGCCACCGTGTTCGAGCAGTTTCACCACAAATTCAGCAAGACAAGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

592

Amino Acids

66.5

Weight (kDa)

5.23

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 147 4.4e-40 Multicopper oxidase
Cu-oxidase PF00394 163 - 315 1.1e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 439 - 553 3.3e-38 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1732
Acc36I ACCTGC 2 cut(s) 443, 1732
AccB1I GGYRCC 2 cut(s) 422, 1118
AccB7I CCANNNNNTGG 1 cut(s) 1715
AccI GTMKAC 1 cut(s) 960
AciI CCGC 3 cut(s) 828, 879, 1010
AclWI GGATC 6 cut(s) 508, 763, 1419, 1462, 1505, 1620
AcoI YGGCCR 1 cut(s) 844
AcsI RAATTY 2 cut(s) 334, 1754
AcuI CTGAAG 3 cut(s) 32, 1230, 1512
AfaI GTAC 5 cut(s) 102, 264, 634, 1355, 1387
AfiI CCNNNNNNNGG 3 cut(s) 1221, 1547, 1715
AflII CTTAAG 1 cut(s) 1031
AgsI TTSAA 4 cut(s) 74, 518, 896, 1570
AjnI CCWGG 3 cut(s) 169, 601, 1582
AleI CACNNNNGTG 1 cut(s) 379
AluBI AGCT 3 cut(s) 410, 1038, 1440
AluI AGCT 3 cut(s) 410, 1038, 1440
Alw21I GWGCWC 1 cut(s) 623
Alw26I GTCTC 5 cut(s) 192, 225, 600, 1475, 1667
AlwI GGATC 6 cut(s) 508, 763, 1419, 1462, 1505, 1620
AlwNI CAGNNNCTG 1 cut(s) 1490
AoxI GGCC 4 cut(s) 111, 238, 844, 1153
ApoI RAATTY 2 cut(s) 334, 1754
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AseI ATTAAT 2 cut(s) 591, 1128
AspS9I GGNCC 3 cut(s) 29, 173, 293
AsuC2I CCSGG 1 cut(s) 956
AsuHPI GGTGA 5 cut(s) 817, 944, 1148, 1713, 1739
AvaII GGWCC 3 cut(s) 29, 173, 293
BanI GGYRCC 2 cut(s) 422, 1118
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 871
Bbv12I GWGCWC 1 cut(s) 623
BccI CCATC 7 cut(s) 10, 284, 470, 746, 779, 982, 1201
BceAI ACGGC 1 cut(s) 875
BciT130I CCWGG 3 cut(s) 171, 603, 1584
BcnI CCSGG 1 cut(s) 956
BcoDI GTCTC 5 cut(s) 192, 225, 600, 1475, 1667
BfaI CTAG 4 cut(s) 149, 405, 411, 1335
BfmI CTRYAG 1 cut(s) 1219
BfrI CTTAAG 1 cut(s) 1031
BfuAI ACCTGC 2 cut(s) 443, 1732
BlpI GCTNAGC 2 cut(s) 468, 1039
Bme1390I CCNGG 4 cut(s) 171, 603, 956, 1584
Bme18I GGWCC 3 cut(s) 29, 173, 293
BmgT120I GGNCC 3 cut(s) 29, 173, 293
BmiI GGNNCC 3 cut(s) 424, 1120, 1302
BmrFI CCNGG 4 cut(s) 171, 603, 956, 1584
BmsI GCATC 2 cut(s) 1390, 1412
BpiI GAAGAC 1 cut(s) 871
BpmI CTGGAG 2 cut(s) 624, 950
Bpu1102I GCTNAGC 2 cut(s) 468, 1039
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 956
BsaJI CCNNGG 7 cut(s) 32, 226, 281, 418, 569, 810, 1583
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 1221, 1547, 1715
Bse1I ACTGG 2 cut(s) 257, 1575
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 3 cut(s) 171, 603, 1584
BseDI CCNNGG 7 cut(s) 32, 226, 281, 418, 569, 810, 1583
BseGI GGATG 4 cut(s) 550, 757, 974, 1414
BseLI CCNNNNNNNGG 3 cut(s) 1221, 1547, 1715
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 3 cut(s) 459, 531, 1398
BseNI ACTGG 2 cut(s) 257, 1575
BseRI GAGGAG 1 cut(s) 558
BshFI GGCC 4 cut(s) 113, 240, 846, 1155
BshNI GGYRCC 2 cut(s) 422, 1118
BsiHKAI GWGCWC 1 cut(s) 623
BsiSI CCGG 2 cut(s) 801, 956
BslFI GGGAC 1 cut(s) 1523
BslI CCNNNNNNNGG 3 cut(s) 1221, 1547, 1715
BsmAI GTCTC 5 cut(s) 192, 225, 600, 1475, 1667
BsmBI CGTCTC 1 cut(s) 1667
BsmFI GGGAC 1 cut(s) 1523
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 4 cut(s) 113, 240, 846, 1155
Bsp1286I GDGCHC 2 cut(s) 474, 623
Bsp143I GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
Bsp1720I GCTNAGC 2 cut(s) 468, 1039
Bsp19I CCATGG 2 cut(s) 418, 810
BspACI CCGC 3 cut(s) 828, 879, 1010
BspANI GGCC 4 cut(s) 113, 240, 846, 1155
BspCNI CTCAG 3 cut(s) 460, 532, 1399
BspLI GGNNCC 3 cut(s) 424, 1120, 1302
BspMI ACCTGC 2 cut(s) 443, 1732
BspPI GGATC 6 cut(s) 508, 763, 1419, 1462, 1505, 1620
BspT107I GGYRCC 2 cut(s) 422, 1118
BspTI CTTAAG 1 cut(s) 1031
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 2 cut(s) 257, 1575
BssECI CCNNGG 7 cut(s) 32, 226, 281, 418, 569, 810, 1583
BssMI GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
BssNAI GTATAC 1 cut(s) 961
BssT1I CCWWGG 5 cut(s) 32, 226, 281, 418, 810
Bst1107I GTATAC 1 cut(s) 961
Bst2UI CCWGG 3 cut(s) 171, 603, 1584
Bst4CI ACNGT 8 cut(s) 205, 739, 1102, 1324, 1531, 1604, 1642, 1732
Bst6I CTCTTC 3 cut(s) 441, 1134, 1356
BstAFI CTTAAG 1 cut(s) 1031
BstC8I GCNNGC 7 cut(s) 6, 146, 188, 387, 644, 1045, 1667
BstDEI CTNAG 8 cut(s) 138, 442, 468, 540, 882, 1039, 1178, 1407
BstDSI CCRYGG 2 cut(s) 418, 810
BstEII GGTNACC 1 cut(s) 823
BstENI CCTNNNNNAGG 1 cut(s) 1219
BstF5I GGATG 4 cut(s) 550, 757, 974, 1414
BstKTI GATC 8 cut(s) 503, 758, 1146, 1297, 1414, 1457, 1513, 1615
BstMAI GTCTC 5 cut(s) 192, 225, 600, 1475, 1667
BstMBI GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
BstMWI GCNNNNNNNGC 2 cut(s) 887, 1044
BstNI CCWGG 3 cut(s) 171, 603, 1584
BstNSI RCATGY 3 cut(s) 8, 148, 389
BstPI GGTNACC 1 cut(s) 823
BstSCI CCNGG 4 cut(s) 169, 601, 954, 1582
BstSFI CTRYAG 1 cut(s) 1219
BstV2I GAAGAC 1 cut(s) 871
BstX2I RGATCY 1 cut(s) 500
BstXI CCANNNNNNTGG 2 cut(s) 842, 1590
BstYI RGATCY 1 cut(s) 500
BstZ17I GTATAC 1 cut(s) 961
BsuRI GGCC 4 cut(s) 113, 240, 846, 1155
BtgI CCRYGG 2 cut(s) 418, 810
BtgZI GCGATG 2 cut(s) 623, 1121
BtsCI GGATG 4 cut(s) 550, 757, 974, 1414
BtsIMutI CAGTG 5 cut(s) 250, 735, 1527, 1600, 1638
BveI ACCTGC 2 cut(s) 443, 1732
Cac8I GCNNGC 7 cut(s) 6, 146, 188, 387, 644, 1045, 1667
CaiI CAGNNNCTG 1 cut(s) 1490
Cfr13I GGNCC 3 cut(s) 29, 173, 293
Csp6I GTAC 5 cut(s) 101, 263, 633, 1354, 1386
CviQI GTAC 5 cut(s) 101, 263, 633, 1354, 1386
DdeI CTNAG 8 cut(s) 138, 442, 468, 540, 882, 1039, 1178, 1407
DpnI GATC 8 cut(s) 502, 757, 1145, 1296, 1413, 1456, 1512, 1614
DpnII GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
DraI TTTAAA 1 cut(s) 1170
EaeI YGGCCR 1 cut(s) 844
Eam1104I CTCTTC 3 cut(s) 441, 1134, 1356
EarI CTCTTC 3 cut(s) 441, 1134, 1356
Eco130I CCWWGG 5 cut(s) 32, 226, 281, 418, 810
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 3 cut(s) 29, 173, 293
Eco57I CTGAAG 3 cut(s) 32, 1230, 1512
Eco91I GGTNACC 1 cut(s) 823
EcoNI CCTNNNNNAGG 1 cut(s) 1219
EcoO65I GGTNACC 1 cut(s) 823
EcoRII CCWGG 3 cut(s) 169, 601, 1582
EcoT14I CCWWGG 5 cut(s) 32, 226, 281, 418, 810
EcoT22I ATGCAT 2 cut(s) 6, 1405
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 5 cut(s) 32, 226, 281, 418, 810
Esp3I CGTCTC 1 cut(s) 1667
FalI AAGNNNNNCTT 4 cut(s) 265, 297, 543, 575
FaqI GGGAC 1 cut(s) 1523
FauNDI CATATG 1 cut(s) 760
FblI GTMKAC 1 cut(s) 960
FokI GGATG 4 cut(s) 557, 764, 961, 1401
FriOI GRGCYC 1 cut(s) 474
FspBI CTAG 4 cut(s) 149, 405, 411, 1335
GsuI CTGGAG 2 cut(s) 624, 950
HaeIII GGCC 4 cut(s) 113, 240, 846, 1155
HapII CCGG 2 cut(s) 801, 956
HincII GTYRAC 2 cut(s) 157, 1524
HindII GTYRAC 2 cut(s) 157, 1524
HinfI GANTC 2 cut(s) 1338, 1566
HpaI GTTAAC 1 cut(s) 1524
HpaII CCGG 2 cut(s) 801, 956
HphI GGTGA 5 cut(s) 817, 944, 1148, 1713, 1739
Hpy166II GTNNAC 6 cut(s) 157, 635, 961, 1020, 1524, 1721
Hpy188I TCNGA 9 cut(s) 12, 289, 361, 525, 577, 1249, 1291, 1408, 1565
Hpy188III TCNNGA 6 cut(s) 296, 560, 775, 929, 1256, 1304
Hpy8I GTNNAC 6 cut(s) 157, 635, 961, 1020, 1524, 1721
HpyAV CCTTC 7 cut(s) 68, 362, 548, 890, 992, 1166, 1487
HpyCH4III ACNGT 8 cut(s) 205, 739, 1102, 1324, 1531, 1604, 1642, 1732
HpyCH4IV ACGT 2 cut(s) 520, 665
HpyCH4V TGCA 6 cut(s) 4, 190, 701, 1403, 1423, 1429
HpyF10VI GCNNNNNNNGC 2 cut(s) 887, 1044
HpyF3I CTNAG 8 cut(s) 138, 442, 468, 540, 882, 1039, 1178, 1407
HpySE526I ACGT 2 cut(s) 520, 665
KspAI GTTAAC 1 cut(s) 1524
Kzo9I GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
LmnI GCTCC 1 cut(s) 1306
LweI GCATC 2 cut(s) 1390, 1412
MaeI CTAG 4 cut(s) 149, 405, 411, 1335
MaeII ACGT 2 cut(s) 520, 665
MaeIII GTNAC 6 cut(s) 304, 535, 775, 823, 916, 1318
MalI GATC 8 cut(s) 502, 757, 1145, 1296, 1413, 1456, 1512, 1614
MboI GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
MboII GAAGA 9 cut(s) 25, 458, 494, 563, 662, 665, 876, 1151, 1373
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 2 cut(s) 474, 623
MluCI AATT 9 cut(s) 334, 513, 869, 924, 943, 1371, 1390, 1501, 1754
MlyI GAGTC 1 cut(s) 1347
MmeI TCCRAC 1 cut(s) 1432
Mph1103I ATGCAT 2 cut(s) 6, 1405
MseI TTAA 7 cut(s) 512, 591, 1032, 1128, 1169, 1188, 1523
MslI CAYNNNNRTG 5 cut(s) 90, 255, 379, 1428, 1643
MspCI CTTAAG 1 cut(s) 1031
MspI CCGG 2 cut(s) 801, 956
MspR9I CCNGG 4 cut(s) 171, 603, 956, 1584
MssI GTTTAAAC 1 cut(s) 1170
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 3 cut(s) 171, 603, 1584
MwoI GCNNNNNNNGC 2 cut(s) 887, 1044
NciI CCSGG 1 cut(s) 956
NcoI CCATGG 2 cut(s) 418, 810
NdeI CATATG 1 cut(s) 760
NdeII GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
NlaIV GGNNCC 3 cut(s) 424, 1120, 1302
NmuCI GTSAC 4 cut(s) 304, 775, 823, 916
NsiI ATGCAT 2 cut(s) 6, 1405
NspI RCATGY 3 cut(s) 8, 148, 389
OliI CACNNNNGTG 1 cut(s) 379
PaeI GCATGC 3 cut(s) 8, 148, 389
PaqCI CACCTGC 1 cut(s) 1732
PcsI WCGNNNNNNNCGW 1 cut(s) 1673
PctI GAATGC 1 cut(s) 703
PfeI GAWTC 1 cut(s) 1566
PflFI GACNNNGTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 1715
PfoI TCCNGGA 1 cut(s) 169
PleI GAGTC 1 cut(s) 1346
PmeI GTTTAAAC 1 cut(s) 1170
PpsI GAGTC 1 cut(s) 1346
PshBI ATTAAT 2 cut(s) 591, 1128
Psp6I CCWGG 3 cut(s) 169, 601, 1582
PspEI GGTNACC 1 cut(s) 823
PspGI CCWGG 3 cut(s) 169, 601, 1582
PspN4I GGNNCC 3 cut(s) 424, 1120, 1302
PspPI GGNCC 3 cut(s) 29, 173, 293
PstNI CAGNNNCTG 1 cut(s) 1490
PsuI RGATCY 1 cut(s) 500
PsyI GACNNNGTC 1 cut(s) 203
RsaI GTAC 5 cut(s) 102, 264, 634, 1355, 1387
RsaNI GTAC 5 cut(s) 101, 263, 633, 1354, 1386
RseI CAYNNNNRTG 5 cut(s) 90, 255, 379, 1428, 1643
SaqAI TTAA 7 cut(s) 512, 591, 1032, 1128, 1169, 1188, 1523
Sau3AI GATC 8 cut(s) 500, 755, 1143, 1294, 1411, 1454, 1510, 1612
Sau96I GGNCC 3 cut(s) 29, 173, 293
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 4 cut(s) 171, 603, 956, 1584
SduI GDGCHC 2 cut(s) 474, 623
SfaNI GCATC 2 cut(s) 1390, 1412
SfcI CTRYAG 1 cut(s) 1219
SinI GGWCC 3 cut(s) 29, 173, 293
SmiMI CAYNNNNRTG 5 cut(s) 90, 255, 379, 1428, 1643
SmlI CTYRAG 2 cut(s) 104, 1031
SmoI CTYRAG 2 cut(s) 104, 1031
SphI GCATGC 3 cut(s) 8, 148, 389
Sse9I AATT 9 cut(s) 334, 513, 869, 924, 943, 1371, 1390, 1501, 1754
SsiI CCGC 3 cut(s) 828, 879, 1010
SspI AATATT 2 cut(s) 495, 1275
SspMI CTAG 4 cut(s) 149, 405, 411, 1335
StyD4I CCNGG 4 cut(s) 169, 601, 954, 1582
StyI CCWWGG 5 cut(s) 32, 226, 281, 418, 810
TaaI ACNGT 8 cut(s) 205, 739, 1102, 1324, 1531, 1604, 1642, 1732
TaiI ACGT 2 cut(s) 523, 668
TaqI TCGA 3 cut(s) 549, 1615, 1737
TaqII GACCGA 2 cut(s) 190, 561
TasI AATT 9 cut(s) 334, 513, 869, 924, 943, 1371, 1390, 1501, 1754
TatI WGTACW 4 cut(s) 100, 262, 632, 1353
TfiI GAWTC 1 cut(s) 1566
Tru1I TTAA 7 cut(s) 512, 591, 1032, 1128, 1169, 1188, 1523
Tru9I TTAA 7 cut(s) 512, 591, 1032, 1128, 1169, 1188, 1523
TscAI CASTG 5 cut(s) 257, 742, 1534, 1607, 1645
TseFI GTSAC 4 cut(s) 304, 775, 823, 916
Tsp45I GTSAC 4 cut(s) 304, 775, 823, 916
TspDTI ATGAA 6 cut(s) 74, 642, 710, 1124, 1374, 1649
TspRI CASTG 5 cut(s) 257, 742, 1534, 1607, 1645
Tth111I GACNNNGTC 1 cut(s) 203
Van91I CCANNNNNTGG 1 cut(s) 1715
Vha464I CTTAAG 1 cut(s) 1031
VpaK11BI GGWCC 3 cut(s) 29, 173, 293
VspI ATTAAT 2 cut(s) 591, 1128
XagI CCTNNNNNAGG 1 cut(s) 1219
XapI RAATTY 2 cut(s) 334, 1754
XceI RCATGY 3 cut(s) 8, 148, 389
XcmI CCANNNNNNNNNTGG 4 cut(s) 233, 279, 817, 1548
XmiI GTMKAC 1 cut(s) 960
XspI CTAG 4 cut(s) 149, 405, 411, 1335
Zsp2I ATGCAT 2 cut(s) 6, 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.