Rroxscaffold_1G00022630

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001075
Physical Location & Seq
Reverse (-)
27990120 .. 27996108
5989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022630.1

Sequence Viewer

Length: 1611 bp
ATGGCTCCTTCTACTTTGCTTTCGGTCGAAGGTGGTGGTTGCACGAGTTCTAGGTTCAAGGCGAGTTGTGGTGATCGGTTATGTGGTTGGGGTTGTCCTCCAACGGGTTGTGGATCCGTGCCGGGTTCCAATCGGATTCGGTCGCAAATCCCTTTGAAGCTTAGTGGCGGGGGTGGATCTTGCAGCGGGGAGGGTGTCATGGTGGTGGGGATTGGCTCTTATTGGAATTGGACTACCGAGGGAAGAGGCGGTAGTGTTGCCTCATCCAAGCTACAAGCAGGATGGGTATGTCCCCAGGTTTTCTGGGTTCGTTGCTTCTCTGTGGATGCCTCCTTTGTATCGTTGAAGGAAATGTCCATTACTACGACTTCATTGCATGGAGTACATCAACCAAGAAACCCGTGGTCAGACGGTCCCGAGTACATCACACAGTGTCCCATTAAACCTGGATCAAATTTTACTTATGAGGTTATATTTTCTGAAGAGGAAGGAACATTATGGTGGCATGCACATAGTGAATGGACTAGAGCTAGTGTTCATGGTGCCATTGTTGTGATGCCTTTAAAAGAAACTGGTTTTCCATTTATAGAGCCTGATGGAGAAGAAATCATTGTTTTCGGATCTTGGTATATTATTGAAGATGTAAATGAGGCGGTTGCGGAGGCACTCACAGATGGGGTAGACCCTCTTCGCTCGGATTGCTACACCATTAATGGTCAACCAGGCGATTTTGCCCCATGCTCAATTGAGCTCTATTTTGCGATTGCTGAACATACTCTCACTGTTGTTGGTATTGATGGATCATATGTTAAACCCATCGTGACTGACTATATCATGATTTGCCCTGGGCAAACTATGGATGTTTTGTTGACATCCAATCAGTCTCTAGGCCGATATTACATGGCTGCTAGACAATATGACAGTGGCGGCGGCAATGAAGATTATGACAAAACAAATGTGACTGCGATTTTGGACCGTATTAAGAGTTTGGCGAGCACAAGTCACCCCATAAATGTGCCGGAGAACATTACTACTAGAATGTATATAATCGCTGAGACGAACATGATTACATTTACCAACTTTTCTGGTGAGATGATTGGTAGTATGGCTGCTAGTTTAAATAATGTAAGCTGGGTTAATCCATCAACAGATGTCCTACTTGCATACTACAGGAATATTAGTGGAATTTATACCTTAAACTTTCCTGATTATCCACCAACTATGTTTGATTTTGTGGCTGATGATTTGCCCGATAGTTATATAAATTCAGTTCATGCAACAAAGGTGAAAGTGTTAGAATACAATGAAGAGGTGGAAATAGTGTTTCAGGATACTAATGTATTTGATGTATCTGAGGATCATCCAATGCATTTTCATGGCCATAGCTTTTATGTAGTTGGATCAGGTTATGGGAATTTTGACAATGAAACAGACCCTAAAGGGTATAATTTGGTTGATCCCCCAAAGATGACTACTGTCTCACTTCCAAAGAAAGGATGGGTGGCTATCAGATTCAAAGCAAGTAATCCAGTCGTGGATAATTCTTACAGGGGGTTTGGTTATGGCACTGTCATTATGATCGACATCTCAGCTGGGGCATGGACACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

536

Amino Acids

58.65

Weight (kDa)

4.71

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 121 - 187 1.9e-21 Multicopper oxidase
Cu-oxidase PF00394 247 - 325 3.2e-18 Multicopper oxidase
Cu-oxidase_2 PF07731 401 - 511 1.1e-24 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 542
AccI GTMKAC 1 cut(s) 681
AciI CCGC 7 cut(s) 168, 186, 249, 653, 659, 927, 930
AclWI GGATC 9 cut(s) 108, 121, 184, 457, 628, 808, 1365, 1408, 1451
AcoI YGGCCR 1 cut(s) 1378
AcsI RAATTY 4 cut(s) 454, 1185, 1264, 1414
AcuI CTGAAG 1 cut(s) 501
AdeI CACNNNGTG 2 cut(s) 432, 515
AfaI GTAC 2 cut(s) 384, 422
AfiI CCNNNNNNNGG 2 cut(s) 104, 1493
AgsI TTSAA 5 cut(s) 58, 157, 346, 638, 1516
AjnI CCWGG 4 cut(s) 294, 445, 721, 844
AluBI AGCT 7 cut(s) 160, 271, 530, 751, 1131, 1386, 1592
AluI AGCT 7 cut(s) 160, 271, 530, 751, 1131, 1386, 1592
Alw21I GWGCWC 2 cut(s) 753, 998
Alw26I GTCTC 3 cut(s) 888, 1049, 1483
AlwI GGATC 9 cut(s) 108, 121, 184, 457, 628, 808, 1365, 1408, 1451
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 2 cut(s) 889, 1378
ApeKI GCWGC 3 cut(s) 183, 905, 1109
ApoI RAATTY 4 cut(s) 454, 1185, 1264, 1414
ArsI GACNNNNNNTTYG 2 cut(s) 952, 984
AseI ATTAAT 1 cut(s) 711
AspS9I GGNCC 2 cut(s) 413, 973
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 4 cut(s) 83, 995, 1100, 1297
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 2 cut(s) 413, 973
BaeI ACNNNNGTAYC 2 cut(s) 1323, 1356
BalI TGGCCA 1 cut(s) 1380
BamHI GGATCC 1 cut(s) 113
BanI GGYRCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 753
BarI GAAGNNNNNNTAC 2 cut(s) 672, 704
BauI CACGAG 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 753, 998
BbvI GCAGC 3 cut(s) 195, 892, 1096
BccI CCATC 7 cut(s) 276, 590, 668, 791, 824, 1150, 1491
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BciT130I CCWGG 4 cut(s) 296, 447, 723, 846
BciVI GTATCC 1 cut(s) 1324
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 3 cut(s) 888, 1049, 1483
BfaI CTAG 7 cut(s) 51, 525, 531, 887, 909, 1035, 1113
BfmI CTRYAG 1 cut(s) 1168
BfuI GTATCC 1 cut(s) 1324
BisI GCNGC 5 cut(s) 184, 906, 928, 931, 1110
BlsI GCNGC 5 cut(s) 185, 907, 929, 932, 1111
Bme1390I CCNGG 5 cut(s) 123, 296, 447, 723, 846
Bme18I GGWCC 2 cut(s) 413, 973
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 2 cut(s) 413, 973
BmiI GGNNCC 5 cut(s) 6, 115, 127, 415, 544
BmrFI CCNGG 5 cut(s) 123, 296, 447, 723, 846
BmsI GCATC 2 cut(s) 316, 546
BoxI GACNNNNGTC 1 cut(s) 1475
BpuMI CCSGG 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 1583
BsaJI CCNNGG 5 cut(s) 237, 294, 401, 844, 845
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 1493
Bse1I ACTGG 2 cut(s) 577, 1529
Bse3DI GCAATG 2 cut(s) 371, 940
Bse8I GATNNNNATC 1 cut(s) 1583
BseBI CCWGG 4 cut(s) 296, 447, 723, 846
BseDI CCNNGG 5 cut(s) 237, 294, 401, 844, 845
BseGI GGATG 7 cut(s) 263, 287, 331, 865, 872, 1360, 1502
BseJI GATNNNNATC 1 cut(s) 1583
BseLI CCNNNNNNNGG 2 cut(s) 104, 1493
BseMI GCAATG 2 cut(s) 371, 940
BseMII CTCAG 3 cut(s) 1044, 1344, 1602
BseNI ACTGG 2 cut(s) 577, 1529
BseXI GCAGC 3 cut(s) 195, 892, 1096
BseYI CCCAGC 2 cut(s) 1131, 1592
Bsh1285I CGRYCG 2 cut(s) 27, 143
BshFI GGCC 2 cut(s) 891, 1380
BshNI GGYRCC 1 cut(s) 542
BsiEI CGRYCG 2 cut(s) 27, 143
BsiHKAI GWGCWC 2 cut(s) 753, 998
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 2 cut(s) 122, 1019
BslFI GGGAC 3 cut(s) 276, 399, 420
BslI CCNNNNNNNGG 2 cut(s) 104, 1493
BsmAI GTCTC 3 cut(s) 888, 1049, 1483
BsmBI CGTCTC 1 cut(s) 1049
BsmFI GGGAC 3 cut(s) 276, 399, 420
BsnI GGCC 2 cut(s) 891, 1380
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 753, 998
BspACI CCGC 7 cut(s) 168, 186, 249, 653, 659, 927, 930
BspANI GGCC 2 cut(s) 891, 1380
BspCNI CTCAG 3 cut(s) 1045, 1345, 1601
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 5 cut(s) 6, 115, 127, 415, 544
BspPI GGATC 9 cut(s) 108, 121, 184, 457, 628, 808, 1365, 1408, 1451
BspT107I GGYRCC 1 cut(s) 542
BsrDI GCAATG 2 cut(s) 371, 940
BsrI ACTGG 2 cut(s) 577, 1529
BssECI CCNNGG 5 cut(s) 237, 294, 401, 844, 845
BssSI CACGAG 1 cut(s) 43
Bst2BI CACGAG 1 cut(s) 43
Bst2UI CCWGG 4 cut(s) 296, 447, 723, 846
Bst4CI ACNGT 8 cut(s) 413, 432, 784, 923, 977, 1477, 1570, 1608
Bst6I CTCTTC 4 cut(s) 238, 477, 693, 1302
BstC8I GCNNGC 2 cut(s) 507, 994
BstDEI CTNAG 4 cut(s) 161, 1053, 1353, 1588
BstDSI CCRYGG 1 cut(s) 401
BstF5I GGATG 7 cut(s) 263, 287, 331, 865, 872, 1360, 1502
BstMAI GTCTC 3 cut(s) 888, 1049, 1483
BstMCI CGRYCG 2 cut(s) 27, 143
BstMWI GCNNNNNNNGC 1 cut(s) 699
BstNI CCWGG 4 cut(s) 296, 447, 723, 846
BstNSI RCATGY 1 cut(s) 509
BstPAI GACNNNNGTC 1 cut(s) 1475
BstSCI CCNGG 5 cut(s) 121, 294, 445, 721, 844
BstSFI CTRYAG 1 cut(s) 1168
BstV1I GCAGC 3 cut(s) 195, 892, 1096
BstX2I RGATCY 3 cut(s) 113, 176, 620
BstYI RGATCY 3 cut(s) 113, 176, 620
BsuI GTATCC 1 cut(s) 1324
BsuRI GGCC 2 cut(s) 891, 1380
BtgI CCRYGG 1 cut(s) 401
BtsCI GGATG 7 cut(s) 263, 287, 331, 865, 872, 1360, 1502
BtsIMutI CAGTG 5 cut(s) 437, 780, 928, 1566, 1604
Cac8I GCNNGC 2 cut(s) 507, 994
CciI TCATGA 1 cut(s) 834
Cfr13I GGNCC 2 cut(s) 413, 973
Csp6I GTAC 2 cut(s) 383, 421
CviQI GTAC 2 cut(s) 383, 421
DdeI CTNAG 4 cut(s) 161, 1053, 1353, 1588
DraI TTTAAA 2 cut(s) 564, 1119
DraIII CACNNNGTG 2 cut(s) 432, 515
EaeI YGGCCR 1 cut(s) 1378
Eam1104I CTCTTC 4 cut(s) 238, 477, 693, 1302
EarI CTCTTC 4 cut(s) 238, 477, 693, 1302
Ecl136II GAGCTC 1 cut(s) 751
Eco24I GRGCYC 1 cut(s) 753
Eco47I GGWCC 2 cut(s) 413, 973
Eco53kI GAGCTC 1 cut(s) 751
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 751
EcoRII CCWGG 4 cut(s) 294, 445, 721, 844
EcoT22I ATGCAT 1 cut(s) 1371
EcoT38I GRGCYC 1 cut(s) 753
Esp3I CGTCTC 1 cut(s) 1049
FaqI GGGAC 3 cut(s) 276, 399, 420
FauI CCCGC 2 cut(s) 161, 179
FauNDI CATATG 1 cut(s) 805
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 5 cut(s) 184, 906, 928, 931, 1110
FokI GGATG 7 cut(s) 250, 294, 338, 859, 872, 1347, 1509
FriOI GRGCYC 1 cut(s) 753
Fsp4HI GCNGC 5 cut(s) 184, 906, 928, 931, 1110
FspBI CTAG 7 cut(s) 51, 525, 531, 887, 909, 1035, 1113
GluI GCNGC 5 cut(s) 184, 906, 928, 931, 1110
GsaI CCCAGC 2 cut(s) 1135, 1596
HaeIII GGCC 2 cut(s) 891, 1380
HapII CCGG 2 cut(s) 122, 1019
HincII GTYRAC 2 cut(s) 719, 870
HindII GTYRAC 2 cut(s) 719, 870
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 136, 1512
HpaII CCGG 2 cut(s) 122, 1019
HphI GGTGA 4 cut(s) 83, 995, 1100, 1297
Hpy166II GTNNAC 3 cut(s) 682, 719, 870
Hpy188I TCNGA 7 cut(s) 135, 409, 481, 620, 697, 1354, 1511
Hpy188III TCNNGA 5 cut(s) 416, 820, 835, 1205, 1328
Hpy8I GTNNAC 3 cut(s) 682, 719, 870
HpyAV CCTTC 4 cut(s) 18, 23, 340, 482
HpyCH4III ACNGT 8 cut(s) 413, 432, 784, 923, 977, 1477, 1570, 1608
HpyCH4V TGCA 7 cut(s) 42, 183, 376, 509, 1163, 1277, 1369
HpyF10VI GCNNNNNNNGC 1 cut(s) 699
HpyF3I CTNAG 4 cut(s) 161, 1053, 1353, 1588
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 3 cut(s) 195, 892, 1096
LweI GCATC 2 cut(s) 316, 546
MaeI CTAG 7 cut(s) 51, 525, 531, 887, 909, 1035, 1113
MaeIII GTNAC 3 cut(s) 820, 958, 1001
MboII GAAGA 7 cut(s) 255, 494, 614, 650, 680, 950, 1319
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 3 cut(s) 113, 176, 620
MhlI GDGCHC 2 cut(s) 753, 998
MlsI TGGCCA 1 cut(s) 1380
MluCI AATT 8 cut(s) 226, 454, 744, 1185, 1264, 1414, 1447, 1540
MluNI TGGCCA 1 cut(s) 1380
MmeI TCCRAC 2 cut(s) 125, 1378
Mox20I TGGCCA 1 cut(s) 1380
Mph1103I ATGCAT 1 cut(s) 1371
MscI TGGCCA 1 cut(s) 1380
MseI TTAA 8 cut(s) 441, 563, 711, 810, 981, 1118, 1137, 1196
MslI CAYNNNNRTG 5 cut(s) 203, 499, 551, 1013, 1374
Msp20I TGGCCA 1 cut(s) 1380
MspA1I CMGCKG 2 cut(s) 186, 1592
MspI CCGG 2 cut(s) 122, 1019
MspR9I CCNGG 5 cut(s) 123, 296, 447, 723, 846
MunI CAATTG 1 cut(s) 744
MvaI CCWGG 4 cut(s) 296, 447, 723, 846
MwoI GCNNNNNNNGC 1 cut(s) 699
NciI CCSGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 805
NlaIV GGNNCC 5 cut(s) 6, 115, 127, 415, 544
NmuCI GTSAC 3 cut(s) 820, 958, 1001
NsiI ATGCAT 1 cut(s) 1371
NspI RCATGY 1 cut(s) 509
PaeI GCATGC 1 cut(s) 509
PagI TCATGA 1 cut(s) 834
PasI CCCWGGG 1 cut(s) 845
PfeI GAWTC 2 cut(s) 136, 1512
PkrI GCNGC 5 cut(s) 185, 907, 929, 932, 1111
PshAI GACNNNNGTC 1 cut(s) 1475
PshBI ATTAAT 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 753
Psp6I CCWGG 4 cut(s) 294, 445, 721, 844
PspFI CCCAGC 2 cut(s) 1131, 1592
PspGI CCWGG 4 cut(s) 294, 445, 721, 844
PspN4I GGNNCC 5 cut(s) 6, 115, 127, 415, 544
PspPI GGNCC 2 cut(s) 413, 973
PsuI RGATCY 3 cut(s) 113, 176, 620
PvuII CAGCTG 1 cut(s) 1592
RsaI GTAC 2 cut(s) 384, 422
RsaNI GTAC 2 cut(s) 383, 421
RseI CAYNNNNRTG 5 cut(s) 203, 499, 551, 1013, 1374
SacI GAGCTC 1 cut(s) 753
SaqAI TTAA 8 cut(s) 441, 563, 711, 810, 981, 1118, 1137, 1196
SatI GCNGC 5 cut(s) 184, 906, 928, 931, 1110
Sau96I GGNCC 2 cut(s) 413, 973
ScrFI CCNGG 5 cut(s) 123, 296, 447, 723, 846
SduI GDGCHC 2 cut(s) 753, 998
SfaNI GCATC 2 cut(s) 316, 546
SfcI CTRYAG 1 cut(s) 1168
SinI GGWCC 2 cut(s) 413, 973
SmiMI CAYNNNNRTG 5 cut(s) 203, 499, 551, 1013, 1374
SphI GCATGC 1 cut(s) 509
Sse9I AATT 8 cut(s) 226, 454, 744, 1185, 1264, 1414, 1447, 1540
SsiI CCGC 7 cut(s) 168, 186, 249, 653, 659, 927, 930
SspI AATATT 1 cut(s) 1177
SspMI CTAG 7 cut(s) 51, 525, 531, 887, 909, 1035, 1113
SstI GAGCTC 1 cut(s) 753
StyD4I CCNGG 5 cut(s) 121, 294, 445, 721, 844
TaaI ACNGT 8 cut(s) 413, 432, 784, 923, 977, 1477, 1570, 1608
TaqI TCGA 2 cut(s) 27, 1581
TaqII GACCGA 2 cut(s) 13, 129
TasI AATT 8 cut(s) 226, 454, 744, 1185, 1264, 1414, 1447, 1540
TatI WGTACW 2 cut(s) 382, 420
TauI GCSGC 2 cut(s) 930, 933
TfiI GAWTC 2 cut(s) 136, 1512
Tru1I TTAA 8 cut(s) 441, 563, 711, 810, 981, 1118, 1137, 1196
Tru9I TTAA 8 cut(s) 441, 563, 711, 810, 981, 1118, 1137, 1196
TscAI CASTG 5 cut(s) 437, 787, 928, 1573, 1611
TseFI GTSAC 3 cut(s) 820, 958, 1001
TseI GCWGC 3 cut(s) 183, 905, 1109
Tsp45I GTSAC 3 cut(s) 820, 958, 1001
TspDTI ATGAA 7 cut(s) 360, 527, 951, 1262, 1320, 1364, 1440
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 5 cut(s) 437, 787, 928, 1573, 1611
VpaK11BI GGWCC 2 cut(s) 413, 973
VspI ATTAAT 1 cut(s) 711
XapI RAATTY 4 cut(s) 454, 1185, 1264, 1414
XceI RCATGY 1 cut(s) 509
XcmI CCANNNNNNNNNTGG 2 cut(s) 399, 1494
XmiI GTMKAC 1 cut(s) 681
XspI CTAG 7 cut(s) 51, 525, 531, 887, 909, 1035, 1113
Zsp2I ATGCAT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.