Rw6G016800

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001075
Physical Location & Seq
Reverse (-)
33090687 .. 33093924
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016800.1

Sequence Viewer

Length: 1803 bp
ATGCATATATATAGAACTAGAACAACTAGTTTCCGCGTGAAGATGGGTGTGCTCCAATGGTTGCTGGGTTCTCTGCTTCTATGCAGTGCACTTCTTTGCATAGTTGAAGGAAATGTTCACTACTATGACTTTGTACTCAAGGAGGCCAACTTTACAAGGTTATGTCGGACTAAGAGCATGCTGGTAGTCAATGACAGTTTTCCAGGGCCAGTCATTCGTGTACAAAAAGGTGATACTGTTTATGTCAATGTCCAAAATCAAGGAGCCTATGGCCTTACTATTCACTGGCATGGTGTGCATCAACCCAGGAACCCTTGGTCAGATGGTCCTGAGTATGTCACGCAGTGTCCAATTGAACCTGGATCAAATTTCACTTACGAGGTTATATTTTCTGATGAGGAAGGAACACTTTGGTGGCATGCTCATAGTGATTGGACCAGAGCTGGTGTCCATGGTGCCATTGTTGTTATGCCCTCAAATGAGACAGGTTTTCCATTTCCCGAGCCAGATGGAGAAGAAATAGTTGTACTTGGTTCTTGGTATTATATTGATAATCTGAATGAGGTGGTTGCTAAGGACGTCGAAGAAGGTTCTGACACTCCTCGCTCTGATTGCTACACCATGAATGGACAACCAGGAGATTTCAGCCTATGCTCAAATGACAGTACATATCGCTGGCAAGTGGAGTATGGCAAGACGTACCTTCTTCGCCTTGTTAGTGCAGTTATGAATGCAGAACTCTACTTTGCTATTGCTGAACATAGCCTCACTGTTGTTGGTTTGGATGGAGCATATGTAAAACCGATTGTGACCGATAATATCATGATTTCCCCCGGACAAACCATGGATATTTTGGTCACAGCTAACCAGTCTCTTGGTCAATACTACATGGCTGCTAGACAATTTGATAGTGTTAGGCCAGATGATCAAGACTATGACAAGTCAAATGTTACAGCAATTCTGGAGTATAAAGGCAATTACACAACCCCAGCATACCCAATTTATCCGAGTACCCTCCCTTCCTATGAGGATTTACTACCTGCAATAGAGTTTACAGACAAAATCAAGAGCTTAGCAAGCAAAGACCACCCAATAAATGTGCCGGTGAATATAACTACCAAAATGTACATAGCGGTTGAGATGAATAATGGTTCTGTGATTGAGGGTTCTGATGATGTGACAGTTGCTCTTCTTGCAAGTTTAAACAATATAAGCTGGGTTAATCCAACTACAGATGTGCTACTTGCCTACTACAGGAACATTAGTGGGTTTTATGCCTCAGACTTCCCAGATTTTCCACCATATATGTATGACTTTGTGAATGAAAATTTGCCTGATAATACTGAGGTTACTGTCAAGGCAACCAAGGTCAAGGTGTTAGAGTACAATGAAGAGGTGGAAATAGTGTTTCAGGGTACTAATACGATTAATGCATCAGAGAATCATCCAATGCACTTGCATGGATATAGCTTTTATGTAGTCGGATCAGGTTTTGGAAATTTTGACAATGTGAGAGATCCCCAAAGCTACAATTTGGTTGATCCACCGAAGATGACCACTATTTCAGTCCCAATGAAAGGATGGCTTGCTATCAGGTTCAAAGCAAGTAATCCTGGGGTTTGGTTATGGCACTGTCATTTGGATCGACATTTCAGTTGGGGCATGAGCTTTGTGATGATAGTGAAGAATGGAGACACACCGGAGAGGTGCATGCGAGAACCTCCTCCATACATGCCTCCCTGCACTGATTCACCTACCATCAGGTTGCGACCTTCCCACAAATTCAGCAGGACGGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

600

Amino Acids

67.76

Weight (kDa)

5.09

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 47 - 160 5.2e-41 Multicopper oxidase
Cu-oxidase PF00394 174 - 326 1.8e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 438 - 564 6e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 582
Acc36I ACCTGC 1 cut(s) 1048
AccB1I GGYRCC 1 cut(s) 455
AccII CGCG 1 cut(s) 36
AciI CCGC 2 cut(s) 34, 1133
AclWI GGATC 5 cut(s) 370, 1492, 1511, 1535, 1650
AcsI RAATTY 4 cut(s) 367, 1327, 1498, 1781
AcyI GRCGYC 1 cut(s) 579
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 9 cut(s) 135, 222, 528, 667, 701, 1012, 1127, 1385, 1417
AfiI CCNNNNNNNGG 2 cut(s) 1254, 1577
AgsI TTSAA 3 cut(s) 107, 356, 1600
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 5 cut(s) 202, 305, 358, 634, 1612
AleI CACNNNNGTG 1 cut(s) 412
AluBI AGCT 7 cut(s) 443, 863, 1071, 1215, 1470, 1527, 1668
AluI AGCT 7 cut(s) 443, 863, 1071, 1215, 1470, 1527, 1668
Alw21I GWGCWC 2 cut(s) 54, 91
Alw26I GTCTC 3 cut(s) 476, 876, 1686
Alw44I GTGCAC 1 cut(s) 87
AlwI GGATC 5 cut(s) 370, 1492, 1511, 1535, 1650
Ama87I CYCGRG 1 cut(s) 500
AoxI GGCC 4 cut(s) 144, 206, 271, 917
ApaLI GTGCAC 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 893
ApoI RAATTY 4 cut(s) 367, 1327, 1498, 1781
AseI ATTAAT 1 cut(s) 1428
AspS9I GGNCC 3 cut(s) 206, 326, 435
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 3 cut(s) 242, 1117, 1743
AvaI CYCGRG 1 cut(s) 500
AvaII GGWCC 2 cut(s) 326, 435
BaeGI GKGCMC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 455
BarI GAAGNNNNNNTAC 2 cut(s) 1003, 1035
Bbv12I GWGCWC 2 cut(s) 54, 91
BbvI GCAGC 1 cut(s) 880
BccI CCATC 6 cut(s) 37, 317, 503, 779, 1575, 1766
BciT130I CCWGG 5 cut(s) 204, 307, 360, 636, 1614
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 476, 876, 1686
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 3 cut(s) 18, 27, 897
BfmI CTRYAG 2 cut(s) 1230, 1252
BfuAI ACCTGC 1 cut(s) 1048
BisI GCNGC 1 cut(s) 894
BlpI GCTNAGC 1 cut(s) 1072
BlsI GCNGC 1 cut(s) 895
Bme1390I CCNGG 6 cut(s) 204, 307, 360, 636, 834, 1614
Bme18I GGWCC 2 cut(s) 326, 435
BmeT110I CYCGRG 1 cut(s) 500
BmgT120I GGNCC 3 cut(s) 206, 326, 435
BmiI GGNNCC 3 cut(s) 265, 311, 457
BmrFI CCNGG 6 cut(s) 204, 307, 360, 636, 834, 1614
BmsI GCATC 2 cut(s) 307, 1442
BpmI CTGGAG 1 cut(s) 983
Bpu10I CCTNAGC 1 cut(s) 573
Bpu1102I GCTNAGC 1 cut(s) 1072
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 579
BsaJI CCNNGG 8 cut(s) 203, 305, 314, 451, 832, 843, 1365, 1613
BsaWI WCCGGW 1 cut(s) 1699
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 2 cut(s) 1254, 1577
Bse118I RCCGGY 1 cut(s) 1102
Bse1I ACTGG 3 cut(s) 209, 290, 868
BseBI CCWGG 5 cut(s) 204, 307, 360, 636, 1614
BseDI CCNNGG 8 cut(s) 203, 305, 314, 451, 832, 843, 1365, 1613
BseGI GGATG 3 cut(s) 790, 1444, 1586
BseLI CCNNNNNNNGG 2 cut(s) 1254, 1577
BseMII CTCAG 3 cut(s) 321, 1293, 1335
BseNI ACTGG 3 cut(s) 209, 290, 868
BseRI GAGGAG 2 cut(s) 591, 1713
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 1 cut(s) 880
BseYI CCCAGC 3 cut(s) 64, 988, 1215
BsgI GTGCAG 2 cut(s) 741, 1726
Bsh1236I CGCG 1 cut(s) 36
BshFI GGCC 4 cut(s) 146, 208, 273, 919
BshNI GGYRCC 1 cut(s) 455
BsiHKAI GWGCWC 2 cut(s) 54, 91
BsiHKCI CYCGRG 1 cut(s) 500
BsiSI CCGG 3 cut(s) 834, 1103, 1700
BslFI GGGAC 1 cut(s) 1553
BslI CCNNNNNNNGG 2 cut(s) 1254, 1577
BsmAI GTCTC 3 cut(s) 476, 876, 1686
BsmFI GGGAC 1 cut(s) 1553
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 4 cut(s) 146, 208, 273, 919
BsoBI CYCGRG 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 54, 91
Bsp1407I TGTACA 2 cut(s) 220, 1125
Bsp143I GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
Bsp1720I GCTNAGC 1 cut(s) 1072
Bsp19I CCATGG 2 cut(s) 451, 843
BspACI CCGC 2 cut(s) 34, 1133
BspANI GGCC 4 cut(s) 146, 208, 273, 919
BspCNI CTCAG 3 cut(s) 322, 1292, 1336
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 3 cut(s) 265, 311, 457
BspMI ACCTGC 1 cut(s) 1048
BspPI GGATC 5 cut(s) 370, 1492, 1511, 1535, 1650
BspQI GCTCTTC 1 cut(s) 1194
BspT107I GGYRCC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 1102
BsrGI TGTACA 2 cut(s) 220, 1125
BsrI ACTGG 3 cut(s) 209, 290, 868
BssAI RCCGGY 1 cut(s) 1102
BssECI CCNNGG 8 cut(s) 203, 305, 314, 451, 832, 843, 1365, 1613
BssMI GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
BssNI GRCGYC 1 cut(s) 579
BssT1I CCWWGG 4 cut(s) 314, 451, 843, 1365
Bst2UI CCWGG 5 cut(s) 204, 307, 360, 636, 1614
Bst4CI ACNGT 7 cut(s) 197, 238, 665, 772, 1183, 1354, 1634
Bst6I CTCTTC 2 cut(s) 1194, 1386
BstACI GRCGYC 1 cut(s) 579
BstAPI GCANNNNNTGC 1 cut(s) 295
BstAUI TGTACA 2 cut(s) 220, 1125
BstC8I GCNNGC 6 cut(s) 179, 420, 677, 1078, 1587, 1712
BstDEI CTNAG 6 cut(s) 171, 330, 573, 1072, 1279, 1344
BstDSI CCRYGG 2 cut(s) 451, 843
BstENI CCTNNNNNAGG 1 cut(s) 1252
BstF5I GGATG 3 cut(s) 790, 1444, 1586
BstFNI CGCG 1 cut(s) 36
BstKTI GATC 6 cut(s) 365, 928, 1487, 1519, 1543, 1645
BstMAI GTCTC 3 cut(s) 476, 876, 1686
BstMBI GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
BstMWI GCNNNNNNNGC 4 cut(s) 295, 612, 1077, 1193
BstNI CCWGG 5 cut(s) 204, 307, 360, 636, 1614
BstNSI RCATGY 4 cut(s) 181, 422, 1714, 1735
BstSCI CCNGG 6 cut(s) 202, 305, 358, 634, 832, 1612
BstSFI CTRYAG 2 cut(s) 1230, 1252
BstSLI GKGCMC 1 cut(s) 91
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 880
BstX2I RGATCY 1 cut(s) 1516
BstXI CCANNNNNNTGG 1 cut(s) 875
BstYI RGATCY 1 cut(s) 1516
BsuRI GGCC 4 cut(s) 146, 208, 273, 919
BtgI CCRYGG 2 cut(s) 451, 843
BtsCI GGATG 3 cut(s) 790, 1444, 1586
BtsI GCAGTG 2 cut(s) 91, 350
BtsIMutI CAGTG 6 cut(s) 91, 283, 350, 768, 1630, 1743
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 6 cut(s) 179, 420, 677, 1078, 1587, 1712
CciI TCATGA 1 cut(s) 822
Cfr10I RCCGGY 1 cut(s) 1102
Cfr13I GGNCC 3 cut(s) 206, 326, 435
Csp6I GTAC 9 cut(s) 134, 221, 527, 666, 700, 1011, 1126, 1384, 1416
CviQI GTAC 9 cut(s) 134, 221, 527, 666, 700, 1011, 1126, 1384, 1416
DdeI CTNAG 6 cut(s) 171, 330, 573, 1072, 1279, 1344
DpnI GATC 6 cut(s) 364, 927, 1486, 1518, 1542, 1644
DpnII GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
DraI TTTAAA 1 cut(s) 1203
DraIII CACNNNGTG 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 1194, 1386
EarI CTCTTC 2 cut(s) 1194, 1386
Eco130I CCWWGG 4 cut(s) 314, 451, 843, 1365
Eco47I GGWCC 2 cut(s) 326, 435
Eco88I CYCGRG 1 cut(s) 500
EcoNI CCTNNNNNAGG 1 cut(s) 1252
EcoRII CCWGG 5 cut(s) 202, 305, 358, 634, 1612
EcoT14I CCWWGG 4 cut(s) 314, 451, 843, 1365
EcoT22I ATGCAT 2 cut(s) 6, 1435
ErhI CCWWGG 4 cut(s) 314, 451, 843, 1365
FalI AAGNNNNNCTT 4 cut(s) 393, 425, 1569, 1601
FaqI GGGAC 1 cut(s) 1553
FauNDI CATATG 1 cut(s) 793
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 1 cut(s) 894
FokI GGATG 3 cut(s) 797, 1431, 1593
Fsp4HI GCNGC 1 cut(s) 894
FspBI CTAG 3 cut(s) 18, 27, 897
GluI GCNGC 1 cut(s) 894
GsaI CCCAGC 3 cut(s) 68, 992, 1219
GsuI CTGGAG 1 cut(s) 983
HaeIII GGCC 4 cut(s) 146, 208, 273, 919
HapII CCGG 3 cut(s) 834, 1103, 1700
Hin1I GRCGYC 1 cut(s) 579
HinfI GANTC 2 cut(s) 1441, 1748
HpaII CCGG 3 cut(s) 834, 1103, 1700
HphI GGTGA 3 cut(s) 242, 1117, 1743
Hpy166II GTNNAC 4 cut(s) 89, 118, 221, 1053
Hpy188III TCNNGA 6 cut(s) 329, 500, 823, 929, 962, 1066
Hpy8I GTNNAC 4 cut(s) 89, 118, 221, 1053
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 6 cut(s) 101, 395, 581, 713, 1029, 1782
HpyCH4III ACNGT 7 cut(s) 197, 238, 665, 772, 1183, 1354, 1634
HpyCH4IV ACGT 2 cut(s) 579, 698
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 612, 1077, 1193
HpyF3I CTNAG 6 cut(s) 171, 330, 573, 1072, 1279, 1344
HpySE526I ACGT 2 cut(s) 579, 698
Hsp92I GRCGYC 1 cut(s) 579
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
LguI GCTCTTC 1 cut(s) 1194
LmnI GCTCC 3 cut(s) 57, 263, 788
Lsp1109I GCAGC 1 cut(s) 880
LweI GCATC 2 cut(s) 307, 1442
MaeI CTAG 3 cut(s) 18, 27, 897
MaeII ACGT 2 cut(s) 579, 698
MaeIII GTNAC 6 cut(s) 337, 808, 856, 949, 1177, 1348
MalI GATC 6 cut(s) 364, 927, 1486, 1518, 1542, 1644
MboI GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
MboII GAAGA 8 cut(s) 52, 527, 596, 698, 1181, 1403, 1561, 1696
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 1 cut(s) 1516
MhlI GDGCHC 2 cut(s) 54, 91
MmeI TCCRAC 3 cut(s) 146, 1250, 1462
Mph1103I ATGCAT 2 cut(s) 6, 1435
MseI TTAA 3 cut(s) 1202, 1221, 1428
MslI CAYNNNNRTG 4 cut(s) 123, 288, 412, 1458
MspI CCGG 3 cut(s) 834, 1103, 1700
MspR9I CCNGG 6 cut(s) 204, 307, 360, 636, 834, 1614
MssI GTTTAAAC 1 cut(s) 1203
MunI CAATTG 1 cut(s) 351
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 5 cut(s) 204, 307, 360, 636, 1614
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 4 cut(s) 295, 612, 1077, 1193
NciI CCSGG 1 cut(s) 834
NcoI CCATGG 2 cut(s) 451, 843
NdeI CATATG 1 cut(s) 793
NdeII GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
NlaIV GGNNCC 3 cut(s) 265, 311, 457
NmuCI GTSAC 4 cut(s) 337, 808, 856, 1177
NsiI ATGCAT 2 cut(s) 6, 1435
NspI RCATGY 4 cut(s) 181, 422, 1714, 1735
OliI CACNNNNGTG 1 cut(s) 412
PaeI GCATGC 3 cut(s) 181, 422, 1714
PagI TCATGA 1 cut(s) 822
PciSI GCTCTTC 1 cut(s) 1194
PctI GAATGC 1 cut(s) 736
PfeI GAWTC 2 cut(s) 1441, 1748
PkrI GCNGC 1 cut(s) 895
PmeI GTTTAAAC 1 cut(s) 1203
PshBI ATTAAT 1 cut(s) 1428
Psp6I CCWGG 5 cut(s) 202, 305, 358, 634, 1612
PspFI CCCAGC 3 cut(s) 64, 988, 1215
PspGI CCWGG 5 cut(s) 202, 305, 358, 634, 1612
PspN4I GGNNCC 3 cut(s) 265, 311, 457
PspPI GGNCC 3 cut(s) 206, 326, 435
PsuI RGATCY 1 cut(s) 1516
RsaI GTAC 9 cut(s) 135, 222, 528, 667, 701, 1012, 1127, 1385, 1417
RsaNI GTAC 9 cut(s) 134, 221, 527, 666, 700, 1011, 1126, 1384, 1416
RseI CAYNNNNRTG 4 cut(s) 123, 288, 412, 1458
SapI GCTCTTC 1 cut(s) 1194
SaqAI TTAA 3 cut(s) 1202, 1221, 1428
SatI GCNGC 1 cut(s) 894
Sau3AI GATC 6 cut(s) 362, 925, 1484, 1516, 1540, 1642
Sau96I GGNCC 3 cut(s) 206, 326, 435
ScrFI CCNGG 6 cut(s) 204, 307, 360, 636, 834, 1614
SduI GDGCHC 2 cut(s) 54, 91
SfaNI GCATC 2 cut(s) 307, 1442
SfcI CTRYAG 2 cut(s) 1230, 1252
SinI GGWCC 2 cut(s) 326, 435
SmiMI CAYNNNNRTG 4 cut(s) 123, 288, 412, 1458
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SpeI ACTAGT 1 cut(s) 26
SphI GCATGC 3 cut(s) 181, 422, 1714
SsiI CCGC 2 cut(s) 34, 1133
SspMI CTAG 3 cut(s) 18, 27, 897
StyD4I CCNGG 6 cut(s) 202, 305, 358, 634, 832, 1612
StyI CCWWGG 4 cut(s) 314, 451, 843, 1365
TaaI ACNGT 7 cut(s) 197, 238, 665, 772, 1183, 1354, 1634
TaiI ACGT 2 cut(s) 582, 701
TaqI TCGA 2 cut(s) 582, 1645
TaqII GACCGA 1 cut(s) 827
TatI WGTACW 6 cut(s) 133, 220, 526, 665, 1125, 1383
TfiI GAWTC 2 cut(s) 1441, 1748
Tru1I TTAA 3 cut(s) 1202, 1221, 1428
Tru9I TTAA 3 cut(s) 1202, 1221, 1428
TscAI CASTG 6 cut(s) 91, 290, 350, 775, 1637, 1750
TseFI GTSAC 4 cut(s) 337, 808, 856, 1177
TseI GCWGC 1 cut(s) 893
Tsp45I GTSAC 4 cut(s) 337, 808, 856, 1177
TspDTI ATGAA 6 cut(s) 638, 743, 1157, 1338, 1404, 1589
TspRI CASTG 6 cut(s) 91, 290, 350, 775, 1637, 1750
VneI GTGCAC 1 cut(s) 87
VpaK11BI GGWCC 2 cut(s) 326, 435
VspI ATTAAT 1 cut(s) 1428
XagI CCTNNNNNAGG 1 cut(s) 1252
XapI RAATTY 4 cut(s) 367, 1327, 1498, 1781
XceI RCATGY 4 cut(s) 181, 422, 1714, 1735
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 1578
XspI CTAG 3 cut(s) 18, 27, 897
ZraI GACGTC 1 cut(s) 580
Zsp2I ATGCAT 2 cut(s) 6, 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.