MD10G1053300.v1.1

Pentatricopeptide repeat-containing protein At5g09450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010070
Physical Location & Seq
Forward (+)
7139216 .. 7141961
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1053300.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGCGTCTCGGTCACTCTTGCGCACTCTCAGACGGAGCAGCGGTGTCAGCAGTGTTAGTCTTGGTGTTGGTAGATTGTTGTCGTCAGGAGGAGCAGGTGCTGTGAATGCTGAGTTTGTCCAAGATTCTGAGGAAAGTGACAACCTGAAGAGCAGAGTCTTCAAGCTCAGGCAGCCCAAGCGAAGCGCCACCAACGTTCTTCAGAGATGGGTCTCTGAAGGCAACCCAATTTCCATTTCGGACCTCCGCCAGATATCGAAGGACCTCAGCAAGTCTCACCGTTACAAGCACGCCCTCGAGATATCCGAGTGGATGGTCAGCCATAATGAATTTGAGCTATCAGATTCTGACTATGCAGTCCGCATTGTCTTGATGACAAAAGTTTTTGGCATCGATGCTGCGGAACGATACTTTGAAGGTCTACCTCTTACTGCAAAAACAAGTGAAACCTGTACTGCTCTCCTCCACTCATATGCTGGGGCAAAATTGACTGAAAAGGCTGAGAACCTCTATGAGAGGATAAAGGAATCTCACCTGTCCCTGAGTGCCCTTACATACAATGAGATGATGACTATGTACATGTCAGTGGGGCAAGTAGAGAAGGTCTCTATAGTTGTGGAAGATCTGAAATGCCACAAGGTTGCACCGGATATCTTCACATACAATCTTTGGATTAGTTCATGTGCTGCAACTCTAAAGATTGATCAACTTCGACAGATTTTAGATGAAATGAGACATGATCCTGGTTTTGGCGAAGGATGGGAGAGATACATTAACCTGGCAAATATATATGTTAGCGTTGGGCATCTTGTGAATGCAGAGTCCAGCACTCTAGTAGTTGAAGCTGAGAAAGTAATCACACAAAGAGAATGGATCACGTATGACTTCCTTATTATTCTGCATGCTGGTTTAGGAAATAAGGAAAGGATTGATCAGATATGGAAATCCTTGATAATGACTGGGCAAAAGATGACAAGTAGAAACTATATTTGCATTCTTTCTTCATACCTAATGCTTGGCCACTTAAAAGAAGTAGGAGAAGTTATTGATCGGTGGAGGCAATCAACTACTACAGATTTTTCTGTTACTGCCTGCAGTAGGGTTTTCAAAGCTTTCACAGACATTGGGTTGACTGAAAAGGCCCACAACTTCGAGATGCTTCTGATTCAGAAGAACTGTGATCCTGCAAATTCGTTAGAGTAG

Protein Analysis

401

Amino Acids

45.17

Weight (kDa)

6.44

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 184 - 229 4.9e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
AasI GACNNNNNNGTC 1 cut(s) 356
Acc16I TGCGCA 1 cut(s) 23
Acc36I ACCTGC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 421
AciI CCGC 4 cut(s) 42, 247, 361, 401
AclI AACGTT 1 cut(s) 195
AclWI GGATC 3 cut(s) 734, 881, 1175
AcoI YGGCCR 1 cut(s) 1018
AcsI RAATTY 2 cut(s) 329, 1189
AcuI CTGAAG 3 cut(s) 167, 185, 237
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 454, 578
AfiI CCNNNNNNNGG 2 cut(s) 749, 1098
AflIII ACRYGT 1 cut(s) 579
AgsI TTSAA 4 cut(s) 163, 416, 842, 1108
AjnI CCWGG 2 cut(s) 742, 777
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AluBI AGCT 4 cut(s) 166, 337, 845, 1112
AluI AGCT 4 cut(s) 166, 337, 845, 1112
Alw26I GTCTC 5 cut(s) 12, 217, 279, 610, 727
AlwI GGATC 3 cut(s) 734, 881, 1175
AlwNI CAGNNNCTG 2 cut(s) 101, 347
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 2 cut(s) 1018, 1140
ApeKI GCWGC 4 cut(s) 39, 172, 398, 686
ApoI RAATTY 2 cut(s) 329, 1189
AspLEI GCGC 2 cut(s) 24, 188
AspS9I GGNCC 3 cut(s) 241, 262, 1141
AsuHPI GGTGA 2 cut(s) 269, 524
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 2 cut(s) 241, 262
BaeGI GKGCMC 1 cut(s) 550
BalI TGGCCA 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 151
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 4 cut(s) 51, 184, 385, 673
BccI CCATC 3 cut(s) 201, 307, 753
BciT130I CCWGG 2 cut(s) 744, 779
BclI TGATCA 2 cut(s) 703, 931
BcoDI GTCTC 5 cut(s) 12, 217, 279, 610, 727
BfaI CTAG 1 cut(s) 833
BfmI CTRYAG 3 cut(s) 609, 1071, 1093
BfoI RGCGCY 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 86
BglII AGATCT 1 cut(s) 622
BisI GCNGC 4 cut(s) 40, 173, 399, 687
BlsI GCNGC 4 cut(s) 41, 174, 400, 688
Bme1390I CCNGG 2 cut(s) 744, 779
Bme18I GGWCC 2 cut(s) 241, 262
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 3 cut(s) 241, 262, 1141
BmrFI CCNGG 2 cut(s) 744, 779
BmrI ACTGGG 1 cut(s) 969
BmsI GCATC 4 cut(s) 385, 399, 814, 1146
BmuI ACTGGG 1 cut(s) 969
BpiI GAAGAC 1 cut(s) 151
BplI GAGNNNNNCTC 2 cut(s) 590, 622
Bpu10I CCTNAGC 2 cut(s) 167, 266
Bsa29I ATCGAT 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 879
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 217, 610
BsaWI WCCGGW 1 cut(s) 646
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 2 cut(s) 749, 1098
Bse1I ACTGG 1 cut(s) 964
BseBI CCWGG 2 cut(s) 744, 779
BseCI ATCGAT 1 cut(s) 393
BseGI GGATG 2 cut(s) 318, 764
BseLI CCNNNNNNNGG 2 cut(s) 749, 1098
BseMII CTCAG 8 cut(s) 43, 102, 120, 181, 280, 492, 533, 837
BseNI ACTGG 1 cut(s) 964
BseRI GAGGAG 2 cut(s) 105, 452
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 4 cut(s) 51, 184, 385, 673
BseYI CCCAGC 1 cut(s) 476
BshFI GGCC 2 cut(s) 1020, 1142
BshVI ATCGAT 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 1 cut(s) 647
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 2 cut(s) 749, 1098
BsmAI GTCTC 5 cut(s) 12, 217, 279, 610, 727
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 523
BsmI GAATGC 3 cut(s) 112, 820, 993
BsnI GGCC 2 cut(s) 1020, 1142
Bso31I GGTCTC 2 cut(s) 217, 610
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
BspACI CCGC 4 cut(s) 42, 247, 361, 401
BspANI GGCC 2 cut(s) 1020, 1142
BspCNI CTCAG 8 cut(s) 42, 103, 121, 180, 279, 493, 534, 838
BspDI ATCGAT 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 1097
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 3 cut(s) 734, 881, 1175
BspQI GCTCTTC 1 cut(s) 143
BspTNI GGTCTC 2 cut(s) 217, 610
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 1 cut(s) 964
BssMI GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 744, 779
Bst4CI ACNGT 2 cut(s) 281, 1178
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 879
BstC8I GCNNGC 3 cut(s) 291, 903, 1093
BstDEI CTNAG 8 cut(s) 29, 111, 129, 167, 266, 501, 542, 846
BstENI CCTNNNNNAGG 1 cut(s) 1096
BstF5I GGATG 2 cut(s) 318, 764
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 24, 188
BstKTI GATC 7 cut(s) 625, 706, 742, 876, 934, 1051, 1183
BstMAI GTCTC 5 cut(s) 12, 217, 279, 610, 727
BstMBI GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
BstMWI GCNNNNNNNGC 4 cut(s) 48, 107, 172, 178
BstNI CCWGG 2 cut(s) 744, 779
BstNSI RCATGY 2 cut(s) 583, 905
BstSCI CCNGG 2 cut(s) 742, 777
BstSFI CTRYAG 3 cut(s) 609, 1071, 1093
BstSLI GKGCMC 1 cut(s) 550
BstV1I GCAGC 4 cut(s) 51, 184, 385, 673
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
Bsu15I ATCGAT 1 cut(s) 393
BsuRI GGCC 2 cut(s) 1020, 1142
BsuTUI ATCGAT 1 cut(s) 393
BtsCI GGATG 2 cut(s) 318, 764
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 2 cut(s) 58, 591
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 3 cut(s) 291, 903, 1093
CaiI CAGNNNCTG 2 cut(s) 101, 347
CfoI GCGC 2 cut(s) 24, 188
Cfr13I GGNCC 3 cut(s) 241, 262, 1141
ClaI ATCGAT 1 cut(s) 393
Csp6I GTAC 2 cut(s) 453, 577
CviAII CATG 4 cut(s) 580, 681, 737, 902
CviJI RGCY 9 cut(s) 166, 175, 321, 337, 500, 845, 1020, 1112, 1142
CviKI_1 RGCY 9 cut(s) 166, 175, 321, 337, 500, 845, 1020, 1112, 1142
CviQI GTAC 2 cut(s) 453, 577
DdeI CTNAG 8 cut(s) 29, 111, 129, 167, 266, 501, 542, 846
DpnI GATC 7 cut(s) 624, 705, 741, 875, 933, 1050, 1182
DpnII GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 1 cut(s) 1018
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 236
Eco31I GGTCTC 2 cut(s) 217, 610
Eco32I GATATC 3 cut(s) 255, 303, 652
Eco47I GGWCC 2 cut(s) 241, 262
Eco57I CTGAAG 3 cut(s) 167, 185, 237
Eco88I CYCGRG 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 1096
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 742, 777
EcoRV GATATC 3 cut(s) 255, 303, 652
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 4 cut(s) 583, 684, 740, 905
FaqI GGGAC 1 cut(s) 523
FatI CATG 4 cut(s) 579, 680, 736, 901
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 2 cut(s) 703, 931
FblI GTMKAC 1 cut(s) 421
Fnu4HI GCNGC 4 cut(s) 40, 173, 399, 687
FokI GGATG 2 cut(s) 325, 771
Fsp4HI GCNGC 4 cut(s) 40, 173, 399, 687
FspBI CTAG 1 cut(s) 833
FspI TGCGCA 1 cut(s) 23
GlaI GCGC 2 cut(s) 23, 187
GluI GCNGC 4 cut(s) 40, 173, 399, 687
GsaI CCCAGC 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 2 cut(s) 1020, 1142
HapII CCGG 1 cut(s) 647
HhaI GCGC 2 cut(s) 24, 188
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 583, 684, 740, 905
Hin6I GCGC 2 cut(s) 22, 186
HinP1I GCGC 2 cut(s) 22, 186
HincII GTYRAC 1 cut(s) 1131
HindII GTYRAC 1 cut(s) 1131
HindIII AAGCTT 1 cut(s) 1110
HinfI GANTC 6 cut(s) 125, 156, 344, 527, 821, 1165
HpaII CCGG 1 cut(s) 647
HphI GGTGA 2 cut(s) 269, 524
Hpy166II GTNNAC 2 cut(s) 422, 1131
Hpy188III TCNNGA 4 cut(s) 87, 298, 370, 1153
Hpy8I GTNNAC 2 cut(s) 422, 1131
HpyAV CCTTC 5 cut(s) 212, 253, 410, 595, 749
HpyCH4III ACNGT 2 cut(s) 281, 1178
HpyCH4IV ACGT 2 cut(s) 195, 878
HpyCH4V TGCA 9 cut(s) 356, 434, 644, 689, 818, 901, 993, 1095, 1187
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 107, 172, 178
HpyF3I CTNAG 8 cut(s) 29, 111, 129, 167, 266, 501, 542, 846
HpySE526I ACGT 2 cut(s) 195, 878
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 583, 684, 740, 905
HspAI GCGC 2 cut(s) 22, 186
Ksp22I TGATCA 2 cut(s) 703, 931
Kzo9I GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 36, 92
Lsp1109I GCAGC 4 cut(s) 51, 184, 385, 673
LweI GCATC 4 cut(s) 385, 399, 814, 1146
MaeI CTAG 1 cut(s) 833
MaeII ACGT 2 cut(s) 195, 878
MaeIII GTNAC 4 cut(s) 12, 137, 281, 1084
MalI GATC 7 cut(s) 624, 705, 741, 875, 933, 1050, 1182
MboI GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
MboII GAAGA 7 cut(s) 151, 160, 191, 632, 646, 993, 1183
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 550
MlsI TGGCCA 1 cut(s) 1020
MluCI AATT 4 cut(s) 228, 329, 485, 1189
MluNI TGGCCA 1 cut(s) 1020
MlyI GAGTC 2 cut(s) 165, 830
Mox20I TGGCCA 1 cut(s) 1020
MscI TGGCCA 1 cut(s) 1020
MseI TTAA 2 cut(s) 774, 1025
MslI CAYNNNNRTG 2 cut(s) 471, 584
Msp20I TGGCCA 1 cut(s) 1020
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 2 cut(s) 744, 779
Mva1269I GAATGC 3 cut(s) 112, 820, 993
MvaI CCWGG 2 cut(s) 744, 779
MwoI GCNNNNNNNGC 4 cut(s) 48, 107, 172, 178
NdeI CATATG 1 cut(s) 472
NdeII GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
NlaIII CATG 4 cut(s) 583, 684, 740, 905
NmuCI GTSAC 2 cut(s) 12, 137
NsbI TGCGCA 1 cut(s) 23
NspI RCATGY 2 cut(s) 583, 905
PaeI GCATGC 1 cut(s) 905
PaeR7I CTCGAG 1 cut(s) 296
PaqCI CACCTGC 1 cut(s) 86
PciI ACATGT 1 cut(s) 579
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 3 cut(s) 112, 820, 993
PfeI GAWTC 4 cut(s) 125, 344, 527, 1165
PkrI GCNGC 4 cut(s) 41, 174, 400, 688
PleI GAGTC 2 cut(s) 164, 829
PpsI GAGTC 2 cut(s) 164, 829
Ppu21I YACGTR 1 cut(s) 879
PpuMI RGGWCCY 1 cut(s) 262
PscI ACATGT 1 cut(s) 579
Psp1406I AACGTT 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 742, 777
PspFI CCCAGC 1 cut(s) 476
PspGI CCWGG 2 cut(s) 742, 777
PspPI GGNCC 3 cut(s) 241, 262, 1141
PspPPI RGGWCCY 1 cut(s) 262
PstI CTGCAG 1 cut(s) 1097
PstNI CAGNNNCTG 2 cut(s) 101, 347
PsuI RGATCY 1 cut(s) 622
RsaI GTAC 2 cut(s) 454, 578
RsaNI GTAC 2 cut(s) 453, 577
RseI CAYNNNNRTG 2 cut(s) 471, 584
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 774, 1025
SatI GCNGC 4 cut(s) 40, 173, 399, 687
Sau3AI GATC 7 cut(s) 622, 703, 739, 873, 931, 1048, 1180
Sau96I GGNCC 3 cut(s) 241, 262, 1141
SchI GAGTC 2 cut(s) 165, 830
ScrFI CCNGG 2 cut(s) 744, 779
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 4 cut(s) 385, 399, 814, 1146
SfcI CTRYAG 3 cut(s) 609, 1071, 1093
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 241, 262
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 2 cut(s) 471, 584
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 905
Sse9I AATT 4 cut(s) 228, 329, 485, 1189
SsiI CCGC 4 cut(s) 42, 247, 361, 401
SspMI CTAG 1 cut(s) 833
StyD4I CCNGG 2 cut(s) 742, 777
TaaI ACNGT 2 cut(s) 281, 1178
TaiI ACGT 2 cut(s) 198, 881
TaqI TCGA 5 cut(s) 257, 297, 393, 712, 1152
TasI AATT 4 cut(s) 228, 329, 485, 1189
TatI WGTACW 2 cut(s) 452, 576
TfiI GAWTC 4 cut(s) 125, 344, 527, 1165
Tru1I TTAA 2 cut(s) 774, 1025
Tru9I TTAA 2 cut(s) 774, 1025
TscAI CASTG 2 cut(s) 58, 591
TseFI GTSAC 2 cut(s) 12, 137
TseI GCWGC 4 cut(s) 39, 172, 398, 686
Tsp45I GTSAC 2 cut(s) 12, 137
TspDTI ATGAA 4 cut(s) 342, 669, 741, 993
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 2 cut(s) 58, 591
VpaK11BI GGWCC 2 cut(s) 241, 262
XagI CCTNNNNNAGG 1 cut(s) 1096
XapI RAATTY 2 cut(s) 329, 1189
XceI RCATGY 2 cut(s) 583, 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 421
XspI CTAG 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.