MD10G1063400.v1.1

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011557
Physical Location & Seq
Forward (+)
8610242 .. 8611775
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1063400.v1.1.491

Sequence Viewer

Length: 561 bp
ATGAATGCAACTGATGATTTGCTGCTTGCAACAAGGGACACTGCCAAGGAACTGAAAACTGGAATCCACATGCATGTTGCAGAGATAGCATACGAGAATCAATTTGTGACAGAGACGCGAAAAGTTGACCATGGAACGATAACACACTTGGAGAATATACAGTTCCTCCAGAACAATTTGCTAGCTGCCCACACAGTTTGGGTTAATCCTTCTCAGGGCGGGGTCAAAGTATCTCATTGCCCTGCCGCTGCGATGCGAATGCTTGGATTTGCACCTATTAGAGAGATGCTGGATGCTGGCATCTGTGTCTCTTTGGGAACAGATGGGGCACCCTCGAATAACAGAATGAGCATAGCCATTTATTCAGGTAGATTAATCTTTTCCTTTTGCTACATATGCAGCATTTCCAGCCTTGTGTACAGTATGCGAACCGAAAATGTAGTCTCTGTAATGTGCAATGGTCTGTGGATTATGAAGGATAAGAAGATTTTGAACGTGGATGAGGTATTCTGTCATACTTTTCGCTTGTTAGACATACAAATGAAGACTAATGGCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

187

Amino Acids

20.64

Weight (kDa)

6.64

Isoelectric Point (pI)

21.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 3 - 118 4.9e-14 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccII CGCG 1 cut(s) 118
AciI CCGC 3 cut(s) 219, 246, 555
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 1 cut(s) 493
AluBI AGCT 1 cut(s) 185
AluI AGCT 1 cut(s) 185
Alw26I GTCTC 3 cut(s) 107, 313, 448
ApeKI GCWGC 4 cut(s) 22, 185, 248, 399
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AseI ATTAAT 1 cut(s) 374
AsuNHI GCTAGC 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 551
BbvI GCAGC 4 cut(s) 9, 172, 235, 411
BccI CCATC 1 cut(s) 317
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BcoDI GTCTC 3 cut(s) 107, 313, 448
BfaI CTAG 1 cut(s) 182
BisI GCNGC 5 cut(s) 23, 186, 246, 249, 400
BlsI GCNGC 5 cut(s) 24, 187, 247, 250, 401
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 4 cut(s) 243, 276, 283, 309
BmtI GCTAGC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 551
BpmI CTGGAG 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 45, 130
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 2 cut(s) 235, 463
BseDI CCNNGG 2 cut(s) 45, 130
BseGI GGATG 2 cut(s) 298, 505
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMI GCAATG 2 cut(s) 235, 463
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 4 cut(s) 9, 172, 235, 411
Bsh1236I CGCG 1 cut(s) 118
BshNI GGYRCC 1 cut(s) 328
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 3 cut(s) 107, 313, 448
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 2 cut(s) 10, 264
Bsp1286I GDGCHC 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 417
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 3 cut(s) 219, 246, 555
BspCNI CTCAG 1 cut(s) 226
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 330
BspOI GCTAGC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 2 cut(s) 235, 463
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 45, 130
BssT1I CCWWGG 2 cut(s) 45, 130
Bst4CI ACNGT 3 cut(s) 162, 196, 422
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 3 cut(s) 27, 183, 298
BstDEI CTNAG 1 cut(s) 213
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 2 cut(s) 298, 505
BstFNI CGCG 1 cut(s) 118
BstMAI GTCTC 3 cut(s) 107, 313, 448
BstMWI GCNNNNNNNGC 3 cut(s) 86, 396, 408
BstNSI RCATGY 2 cut(s) 73, 77
BstSLI GKGCMC 1 cut(s) 331
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 4 cut(s) 9, 172, 235, 411
BstV2I GAAGAC 1 cut(s) 551
BtgI CCRYGG 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 2 cut(s) 298, 505
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 3 cut(s) 27, 183, 298
CseI GACGC 1 cut(s) 124
Csp6I GTAC 1 cut(s) 418
CspCI CAANNNNNGTGG 2 cut(s) 179, 214
CviAII CATG 3 cut(s) 70, 74, 131
CviJI RGCY 3 cut(s) 185, 356, 411
CviKI_1 RGCY 3 cut(s) 185, 356, 411
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 45, 130
EcoT14I CCWWGG 2 cut(s) 45, 130
EcoT22I ATGCAT 1 cut(s) 75
ErhI CCWWGG 2 cut(s) 45, 130
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 3 cut(s) 73, 77, 134
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 69, 73, 130
FauI CCCGC 1 cut(s) 212
FauNDI CATATG 1 cut(s) 395
Fnu4HI GCNGC 5 cut(s) 23, 186, 246, 249, 400
FokI GGATG 2 cut(s) 305, 512
Fsp4HI GCNGC 5 cut(s) 23, 186, 246, 249, 400
FspBI CTAG 1 cut(s) 182
GluI GCNGC 5 cut(s) 23, 186, 246, 249, 400
GsuI CTGGAG 1 cut(s) 152
HgaI GACGC 1 cut(s) 124
Hin1II CATG 3 cut(s) 73, 77, 134
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 2 cut(s) 63, 97
Hpy166II GTNNAC 2 cut(s) 127, 418
Hpy188III TCNNGA 1 cut(s) 169
Hpy8I GTNNAC 2 cut(s) 127, 418
HpyAV CCTTC 2 cut(s) 219, 469
HpyCH4III ACNGT 3 cut(s) 162, 196, 422
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 7 cut(s) 8, 29, 73, 80, 272, 399, 456
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 396, 408
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 3 cut(s) 73, 77, 134
LpnPI CCDG 8 cut(s) 45, 182, 200, 255, 275, 282, 351, 421
Lsp1109I GCAGC 4 cut(s) 9, 172, 235, 411
LweI GCATC 4 cut(s) 243, 276, 283, 309
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 106
MboII GAAGA 2 cut(s) 496, 556
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 2 cut(s) 101, 175
MnlI CCTC 3 cut(s) 176, 343, 496
Mph1103I ATGCAT 1 cut(s) 75
MseI TTAA 2 cut(s) 204, 374
MslI CAYNNNNRTG 2 cut(s) 72, 539
MspA1I CMGCKG 1 cut(s) 248
Mva1269I GAATGC 2 cut(s) 10, 264
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 3 cut(s) 86, 396, 408
NcoI CCATGG 1 cut(s) 130
NdeI CATATG 1 cut(s) 395
NheI GCTAGC 1 cut(s) 181
NlaIII CATG 3 cut(s) 73, 77, 134
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 1 cut(s) 75
NspI RCATGY 2 cut(s) 73, 77
PctI GAATGC 2 cut(s) 10, 264
PfeI GAWTC 2 cut(s) 63, 97
PkrI GCNGC 5 cut(s) 24, 187, 247, 250, 401
PshBI ATTAAT 1 cut(s) 374
PspN4I GGNNCC 1 cut(s) 330
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 2 cut(s) 72, 539
SaqAI TTAA 2 cut(s) 204, 374
SatI GCNGC 5 cut(s) 23, 186, 246, 249, 400
SduI GDGCHC 1 cut(s) 331
SetI ASST 5 cut(s) 187, 277, 370, 498, 507
SfaNI GCATC 4 cut(s) 243, 276, 283, 309
SmiMI CAYNNNNRTG 2 cut(s) 72, 539
Sse9I AATT 2 cut(s) 101, 175
SsiI CCGC 3 cut(s) 219, 246, 555
SspMI CTAG 1 cut(s) 182
StyI CCWWGG 2 cut(s) 45, 130
TaaI ACNGT 3 cut(s) 162, 196, 422
TaiI ACGT 1 cut(s) 498
TaqI TCGA 1 cut(s) 335
TasI AATT 2 cut(s) 101, 175
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 2 cut(s) 63, 97
Tru1I TTAA 2 cut(s) 204, 374
Tru9I TTAA 2 cut(s) 204, 374
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 4 cut(s) 22, 185, 248, 399
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 3 cut(s) 17, 488, 557
TspRI CASTG 1 cut(s) 46
VspI ATTAAT 1 cut(s) 374
XceI RCATGY 2 cut(s) 73, 77
XspI CTAG 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.