Rroxscaffold_5G00371300

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011557
Physical Location & Seq
Forward (+)
52908158 .. 52911806
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371300.1

Sequence Viewer

Length: 1137 bp
ATGACGTGGCTGCACGAACGGATTTGGCCTTACGAATCCAACATGACTGAGGAAGATTCATACATTTCTACTCTGCTTTGTGGAATTGAGCTCATTCACTCTGGTGTAACTTGCTTTGCTGAAGCAGGAGGGCAACATGTGTCAGGAATGGCGAGGGCGGTTGAGTTACTGGGAATACGGGCATGTTTAGCCGAATCCATCATGGACTCTGGTGACGGCCTGCCTACAACTTGGGCTGCTCGGAGTACTGATGATTGTATTCAGTCTCAGAAAGAGCTTTACAAGAAGCACCATAATACAGGTGATGGCCGCATCAGAGTGTGGTTAGGAATTAGGCAGATCATGAATGCAACTGATGGTTTACTACTTGCAACAAGGGACATTGCCAAAAAACTCAAAACTGGAATCCACATGCATGTTGCAGAGATAGCATATGAGAATCAGTTTGTGACAGATACGCGAAAAGTTAACCATGGAACAGTCACACACTTGGAGCAGATAGATTTTCTCCATAACAATTTGCTAGCAGCTCATACAGTTTGGGTCAACGATTCTGAGATTGATTGTCTTTCAAGGGCTGAGGTAAAAGTATCTCACTGTCCTGCTGCTGCAATGCGAATGCTTGGATTTGCTCCTATTAGAGAGATGATTGGTGCTGGAATCTGTGTCTCCTTGGGAACAGATGGGGCCCCATCAAATAATAGAATGAGCTTAGTTGATGAGATGTACCTGGCCTCTCTGATTAACAAAGGACGGGAAGTTTACACGAATGGAACCACTGATCCTACTGCTCTTCCTTCTGAAACAGTTCTCAAGATGGCGACTATAAATGGTGCAAAATCTGTACTCTGGGATAATGAAATAGGGTCCCTTGAAGTTGGGAAAAAGGCTGACCTGGTCGTTATCAATCCTTCCACTTGGACAATGATTCCTCTCCATGACTGCATTTCAAGCCTTGTGTATTCTATGCGAACTGAAAATATCGTCTCTGTAATGTGCAATGGCAAGTGGATTATGCAGGATAAGAAGATTCTGAATGTGGATGAGGAACACGTTATTTCAATGGCAAAGCAAGCTTCGGCAGAAATTGTAAAGAGAGCAGGCATCAAAATACCAGACAGGATGAACTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

378

Amino Acids

41.75

Weight (kDa)

5.87

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 4 - 338 6.3e-48 Amidohydrolase family
Amidohydro_3 PF07969 175 - 305 1.4e-12 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 460
AciI CCGC 2 cut(s) 158, 310
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 3 cut(s) 247, 728, 846
AflIII ACRYGT 2 cut(s) 136, 1051
AgsI TTSAA 4 cut(s) 573, 875, 951, 1062
AjiI CACGTC 1 cut(s) 6
AjnI CCWGG 2 cut(s) 729, 894
AleI CACNNNNGTG 1 cut(s) 102
AloI GAACNNNNNNTCC 2 cut(s) 766, 798
AluBI AGCT 5 cut(s) 91, 277, 530, 711, 1076
AluI AGCT 5 cut(s) 91, 277, 530, 711, 1076
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 3 cut(s) 270, 673, 991
AlwI GGATC 1 cut(s) 776
AoxI GGCC 5 cut(s) 26, 217, 307, 687, 732
ApaI GGGCCC 1 cut(s) 691
ApeKI GCWGC 5 cut(s) 10, 236, 527, 605, 608
Asp700I GAANNNNTTC 1 cut(s) 807
AspS9I GGNCC 3 cut(s) 687, 688, 867
AsuHPI GGTGA 2 cut(s) 224, 314
AsuNHI GCTAGC 1 cut(s) 523
AvaII GGWCC 1 cut(s) 867
BaeGI GKGCMC 1 cut(s) 691
BanII GRGCYC 2 cut(s) 93, 691
Bbv12I GWGCWC 1 cut(s) 93
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 4 cut(s) 223, 539, 592, 595
BccI CCATC 6 cut(s) 206, 299, 350, 677, 700, 811
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 731, 896
BcoDI GTCTC 3 cut(s) 270, 673, 991
BfaI CTAG 1 cut(s) 524
BisI GCNGC 6 cut(s) 11, 237, 310, 528, 606, 609
BlsI GCNGC 6 cut(s) 12, 238, 311, 529, 607, 610
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 731, 896
Bme18I GGWCC 1 cut(s) 867
BmgBI CACGTC 1 cut(s) 6
BmgT120I GGNCC 3 cut(s) 687, 688, 867
BmiI GGNNCC 6 cut(s) 688, 689, 690, 775, 868, 869
BmrFI CCNGG 2 cut(s) 731, 896
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 321, 1113
BmtI GCTAGC 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 179
Bpu10I CCTNAGC 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 797
BsaJI CCNNGG 2 cut(s) 472, 672
Bse1I ACTGG 2 cut(s) 174, 406
Bse3DI GCAATG 3 cut(s) 381, 618, 1006
BseBI CCWGG 2 cut(s) 731, 896
BseDI CCNNGG 2 cut(s) 472, 672
BseGI GGATG 2 cut(s) 1048, 1128
BseMI GCAATG 3 cut(s) 381, 618, 1006
BseMII CTCAG 4 cut(s) 39, 281, 546, 570
BseNI ACTGG 2 cut(s) 174, 406
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 4 cut(s) 223, 539, 592, 595
Bsh1236I CGCG 1 cut(s) 460
BshFI GGCC 5 cut(s) 28, 219, 309, 689, 734
BsiHKAI GWGCWC 1 cut(s) 93
BslFI GGGAC 2 cut(s) 392, 853
BsmAI GTCTC 3 cut(s) 270, 673, 991
BsmBI CGTCTC 1 cut(s) 991
BsmFI GGGAC 2 cut(s) 392, 853
BsmI GAATGC 2 cut(s) 352, 624
BsnI GGCC 5 cut(s) 28, 219, 309, 689, 734
Bsp120I GGGCCC 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 93, 691
Bsp143I GATC 2 cut(s) 339, 781
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 2 cut(s) 158, 310
BspANI GGCC 5 cut(s) 28, 219, 309, 689, 734
BspCNI CTCAG 4 cut(s) 40, 280, 547, 571
BspFNI CGCG 1 cut(s) 460
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 6 cut(s) 688, 689, 690, 775, 868, 869
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 1 cut(s) 776
BspQI GCTCTTC 1 cut(s) 798
BsrDI GCAATG 3 cut(s) 381, 618, 1006
BsrI ACTGG 2 cut(s) 174, 406
BssECI CCNNGG 2 cut(s) 472, 672
BssMI GATC 2 cut(s) 339, 781
BssT1I CCWWGG 2 cut(s) 472, 672
Bst2UI CCWGG 2 cut(s) 731, 896
Bst4CI ACNGT 4 cut(s) 481, 538, 599, 808
Bst6I CTCTTC 1 cut(s) 798
BstC8I GCNNGC 4 cut(s) 221, 525, 1074, 1102
BstDEI CTNAG 5 cut(s) 48, 267, 555, 579, 712
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 2 cut(s) 1048, 1128
BstFNI CGCG 1 cut(s) 460
BstKTI GATC 2 cut(s) 342, 784
BstMAI GTCTC 3 cut(s) 270, 673, 991
BstMBI GATC 2 cut(s) 339, 781
BstMWI GCNNNNNNNGC 4 cut(s) 188, 428, 951, 1073
BstNI CCWGG 2 cut(s) 731, 896
BstNSI RCATGY 4 cut(s) 140, 186, 415, 419
BstSCI CCNGG 2 cut(s) 729, 894
BstSLI GKGCMC 1 cut(s) 691
BstUI CGCG 1 cut(s) 460
BstV1I GCAGC 4 cut(s) 223, 539, 592, 595
BsuRI GGCC 5 cut(s) 28, 219, 309, 689, 734
BtgI CCRYGG 1 cut(s) 472
BtrI CACGTC 1 cut(s) 6
BtsCI GGATG 2 cut(s) 1048, 1128
BtsIMutI CAGTG 2 cut(s) 595, 777
Cac8I GCNNGC 4 cut(s) 221, 525, 1074, 1102
CciI TCATGA 1 cut(s) 342
Cfr13I GGNCC 3 cut(s) 687, 688, 867
CsiI ACCWGGT 1 cut(s) 894
Csp6I GTAC 3 cut(s) 246, 727, 845
CviAII CATG 9 cut(s) 43, 137, 183, 202, 343, 412, 416, 473, 938
CviQI GTAC 3 cut(s) 246, 727, 845
DdeI CTNAG 5 cut(s) 48, 267, 555, 579, 712
DpnI GATC 2 cut(s) 341, 783
DpnII GATC 2 cut(s) 339, 781
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 472, 672
Eco24I GRGCYC 2 cut(s) 93, 691
Eco47I GGWCC 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 3 cut(s) 687, 688, 867
EcoRII CCWGG 2 cut(s) 729, 894
EcoT14I CCWWGG 2 cut(s) 472, 672
EcoT22I ATGCAT 1 cut(s) 417
EcoT38I GRGCYC 2 cut(s) 93, 691
ErhI CCWWGG 2 cut(s) 472, 672
Esp3I CGTCTC 1 cut(s) 991
FaeI CATG 9 cut(s) 46, 140, 186, 205, 346, 415, 419, 476, 941
FaqI GGGAC 2 cut(s) 392, 853
FatI CATG 9 cut(s) 42, 136, 182, 201, 342, 411, 415, 472, 937
FauNDI CATATG 1 cut(s) 433
Fnu4HI GCNGC 6 cut(s) 11, 237, 310, 528, 606, 609
FokI GGATG 1 cut(s) 1055
FriOI GRGCYC 2 cut(s) 93, 691
Fsp4HI GCNGC 6 cut(s) 11, 237, 310, 528, 606, 609
FspBI CTAG 1 cut(s) 524
GluI GCNGC 6 cut(s) 11, 237, 310, 528, 606, 609
HaeIII GGCC 5 cut(s) 28, 219, 309, 689, 734
Hin1II CATG 9 cut(s) 46, 140, 186, 205, 346, 415, 419, 476, 941
HincII GTYRAC 2 cut(s) 469, 547
HindII GTYRAC 2 cut(s) 469, 547
HindIII AAGCTT 1 cut(s) 1074
HpaI GTTAAC 1 cut(s) 469
HphI GGTGA 2 cut(s) 224, 314
Hpy166II GTNNAC 4 cut(s) 362, 469, 547, 763
Hpy188I TCNGA 7 cut(s) 243, 270, 317, 556, 741, 802, 1035
Hpy188III TCNNGA 3 cut(s) 144, 343, 814
Hpy8I GTNNAC 4 cut(s) 362, 469, 547, 763
HpyAV CCTTC 2 cut(s) 807, 921
HpyCH4III ACNGT 4 cut(s) 481, 538, 599, 808
HpyCH4IV ACGT 2 cut(s) 5, 1053
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 428, 951, 1073
HpyF3I CTNAG 5 cut(s) 48, 267, 555, 579, 712
HpySE526I ACGT 2 cut(s) 5, 1053
Hsp92II CATG 9 cut(s) 46, 140, 186, 205, 346, 415, 419, 476, 941
KflI GGGWCCC 1 cut(s) 867
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 2 cut(s) 339, 781
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 2 cut(s) 493, 637
Lsp1109I GCAGC 4 cut(s) 223, 539, 592, 595
LweI GCATC 2 cut(s) 321, 1113
MabI ACCWGGT 1 cut(s) 894
MaeI CTAG 1 cut(s) 524
MaeII ACGT 2 cut(s) 5, 1053
MaeIII GTNAC 5 cut(s) 106, 165, 212, 448, 481
MalI GATC 2 cut(s) 341, 783
MboI GATC 2 cut(s) 339, 781
MboII GAAGA 3 cut(s) 65, 785, 1039
MhlI GDGCHC 2 cut(s) 93, 691
MluCI AATT 4 cut(s) 84, 330, 517, 1086
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 7 cut(s) 43, 122, 147, 574, 745, 942, 1039
Mph1103I ATGCAT 1 cut(s) 417
MroXI GAANNNNTTC 1 cut(s) 807
MseI TTAA 2 cut(s) 468, 744
MslI CAYNNNNRTG 3 cut(s) 102, 317, 414
MspR9I CCNGG 2 cut(s) 731, 896
Mva1269I GAATGC 2 cut(s) 352, 624
MvaI CCWGG 2 cut(s) 731, 896
MvnI CGCG 1 cut(s) 460
MwoI GCNNNNNNNGC 4 cut(s) 188, 428, 951, 1073
NcoI CCATGG 1 cut(s) 472
NdeI CATATG 1 cut(s) 433
NdeII GATC 2 cut(s) 339, 781
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 9 cut(s) 46, 140, 186, 205, 346, 415, 419, 476, 941
NlaIV GGNNCC 6 cut(s) 688, 689, 690, 775, 868, 869
NmuCI GTSAC 3 cut(s) 212, 448, 481
NsiI ATGCAT 1 cut(s) 417
NspI RCATGY 4 cut(s) 140, 186, 415, 419
OliI CACNNNNGTG 1 cut(s) 102
PagI TCATGA 1 cut(s) 342
PciI ACATGT 1 cut(s) 136
PciSI GCTCTTC 1 cut(s) 798
PctI GAATGC 2 cut(s) 352, 624
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 9 cut(s) 35, 56, 194, 405, 439, 551, 660, 928, 1030
PflFI GACNNNGTC 1 cut(s) 896
PkrI GCNGC 6 cut(s) 12, 238, 311, 529, 607, 610
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 867
PscI ACATGT 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 867
Psp6I CCWGG 2 cut(s) 729, 894
PspGI CCWGG 2 cut(s) 729, 894
PspN4I GGNNCC 6 cut(s) 688, 689, 690, 775, 868, 869
PspOMI GGGCCC 1 cut(s) 687
PspPI GGNCC 3 cut(s) 687, 688, 867
PspPPI RGGWCCY 1 cut(s) 867
PsyI GACNNNGTC 1 cut(s) 896
RsaI GTAC 3 cut(s) 247, 728, 846
RsaNI GTAC 3 cut(s) 246, 727, 845
RseI CAYNNNNRTG 3 cut(s) 102, 317, 414
SacI GAGCTC 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 2 cut(s) 468, 744
SatI GCNGC 6 cut(s) 11, 237, 310, 528, 606, 609
Sau3AI GATC 2 cut(s) 339, 781
Sau96I GGNCC 3 cut(s) 687, 688, 867
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 731, 896
SduI GDGCHC 2 cut(s) 93, 691
SexAI ACCWGGT 1 cut(s) 894
SfaNI GCATC 2 cut(s) 321, 1113
SinI GGWCC 1 cut(s) 867
SmiMI CAYNNNNRTG 3 cut(s) 102, 317, 414
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 4 cut(s) 84, 330, 517, 1086
SsiI CCGC 2 cut(s) 158, 310
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 729, 894
StyI CCWWGG 2 cut(s) 472, 672
TaaI ACNGT 4 cut(s) 481, 538, 599, 808
TaiI ACGT 2 cut(s) 8, 1056
TasI AATT 4 cut(s) 84, 330, 517, 1086
TatI WGTACW 2 cut(s) 245, 844
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 9 cut(s) 35, 56, 194, 405, 439, 551, 660, 928, 1030
Tru1I TTAA 2 cut(s) 468, 744
Tru9I TTAA 2 cut(s) 468, 744
TscAI CASTG 2 cut(s) 602, 784
TseFI GTSAC 3 cut(s) 212, 448, 481
TseI GCWGC 5 cut(s) 10, 236, 527, 605, 608
Tsp45I GTSAC 3 cut(s) 212, 448, 481
TspDTI ATGAA 3 cut(s) 48, 359, 873
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 2 cut(s) 602, 784
Tth111I GACNNNGTC 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 867
XceI RCATGY 4 cut(s) 140, 186, 415, 419
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 1 cut(s) 524
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.